Rw5G024550

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007352
Physical Location & Seq
Forward (+)
34889992 .. 34891852
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G024550.1

Sequence Viewer

Length: 753 bp
ATGAGGAGGAGACCTGGTATTGGTGGTTTGCAGAATGCTGCTGCTGCTAGGGACCAATATCGGGCGCTGGGCCAGAATGTGGCCCAGATTAGAACTGATCTCATGAAGGAACAGCTTGCCACTTTTAAAAGCCAATTAGAAGATTTTGCTCGCAAACACAAGAATGACATTCGCAAGAATCCTGCTTTTAGATCACAATTTCATGATATGTGCACTAAGGTTGGAGTAGACCCCTTGGCCTCCAATAAGGGTTTCTGGGCAGAGCTTTTGGGGATCGGTGACTTCTACTATGAACTTGGAGTGCAAATTGTTGATATCTGCTTAGCAACAAGGCCCCACAATGGAGGCTTGATCAACCTGCAGGAACTCTGTAATTTGCTTCGTCGGAGACGAAAAAATGATCGTGAAGCTGTTTCCGAGGATGATTGCCTGCGTGCTATAAGTAAGCTGAAGGTATTGGGTAATGGTTTTGAGGTTATTTCTGTTGGAAAGAGGAAGCTTGTTCGTTCTGTTCCAACTGAGTTGAACAAAGACCATAATGAAATTTTAGAGCTTGCGCAGTCTCAAGGCTTTGTGACTGTTGATGAGGTAGAAAGACGGCTATCTTGGACCAGTGGCCGTGCAATTGATGCCTTGGATACTTTGCTAGATGAGGGCCTTGCAATGATTGATGATGGCCACAAAGATGGTATACGCCGATATTGGTTTCCCTGTGTATCTTCCATCTCTACCCTAGTTGGAGCTGACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
EAP30

Protein Analysis

250

Amino Acids

28.11

Weight (kDa)

8.58

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAP30 PF04157 6 - 225 1.4e-57 EAP30/Vps36 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 558
Acc36I ACCTGC 1 cut(s) 366
AccB7I CCANNNNNTGG 1 cut(s) 79
AccI GTMKAC 2 cut(s) 228, 691
AclWI GGATC 1 cut(s) 281
AcoI YGGCCR 2 cut(s) 616, 676
AcsI RAATTY 1 cut(s) 543
AcuI CTGAAG 1 cut(s) 470
AfiI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
AgsI TTSAA 1 cut(s) 526
AjnI CCWGG 1 cut(s) 13
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 7 cut(s) 115, 265, 410, 448, 499, 553, 743
AluI AGCT 7 cut(s) 115, 265, 410, 448, 499, 553, 743
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 3 cut(s) 4, 382, 567
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 1 cut(s) 281
AoxI GGCC 7 cut(s) 70, 81, 237, 332, 616, 655, 676
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 3 cut(s) 38, 41, 44
ApoI RAATTY 1 cut(s) 543
AspLEI GCGC 2 cut(s) 67, 559
AspS9I GGNCC 6 cut(s) 52, 70, 82, 333, 609, 655
AsuHPI GGTGA 1 cut(s) 290
AvaII GGWCC 2 cut(s) 52, 609
BaeGI GKGCMC 1 cut(s) 215
BalI TGGCCA 1 cut(s) 678
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 3 cut(s) 25, 28, 31
BccI CCATC 3 cut(s) 668, 680, 731
BceAI ACGGC 2 cut(s) 603, 614
BciT130I CCWGG 1 cut(s) 15
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 3 cut(s) 4, 382, 567
BfaI CTAG 3 cut(s) 48, 647, 734
BfmI CTRYAG 1 cut(s) 359
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 1 cut(s) 366
BfuI GTATCC 1 cut(s) 631
BisI GCNGC 3 cut(s) 39, 42, 45
BlpI GCTNAGC 1 cut(s) 322
BlsI GCNGC 3 cut(s) 40, 43, 46
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 2 cut(s) 52, 609
BmgT120I GGNCC 6 cut(s) 52, 70, 82, 333, 609, 655
BmiI GGNNCC 2 cut(s) 53, 335
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 619
Bpu1102I GCTNAGC 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 549
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 3 cut(s) 234, 417, 633
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
Bse1I ACTGG 1 cut(s) 612
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 3 cut(s) 234, 417, 633
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 1 cut(s) 612
BseRI GAGGAG 2 cut(s) 19, 22
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 3 cut(s) 25, 28, 31
BseYI CCCAGC 1 cut(s) 67
BshFI GGCC 7 cut(s) 72, 83, 239, 334, 618, 657, 678
BsiHKAI GWGCWC 1 cut(s) 215
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
BsmAI GTCTC 3 cut(s) 4, 382, 567
BsmBI CGTCTC 1 cut(s) 382
BsmFI GGGAC 1 cut(s) 65
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 7 cut(s) 72, 83, 239, 334, 618, 657, 678
Bso31I GGTCTC 1 cut(s) 4
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 5 cut(s) 97, 191, 273, 351, 400
Bsp1720I GCTNAGC 1 cut(s) 322
BspANI GGCC 7 cut(s) 72, 83, 239, 334, 618, 657, 678
BspCNI CTCAG 1 cut(s) 511
BspHI TCATGA 2 cut(s) 102, 202
BspLI GGNNCC 2 cut(s) 53, 335
BspMAI CTGCAG 1 cut(s) 363
BspMI ACCTGC 1 cut(s) 366
BspPI GGATC 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 669
BsrI ACTGG 1 cut(s) 612
BssECI CCNNGG 3 cut(s) 234, 417, 633
BssMI GATC 5 cut(s) 97, 191, 273, 351, 400
BssNAI GTATAC 1 cut(s) 692
BssT1I CCWWGG 2 cut(s) 234, 633
Bst1107I GTATAC 1 cut(s) 692
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 580
BstAPI GCANNNNNTGC 1 cut(s) 629
BstC8I GCNNGC 5 cut(s) 117, 151, 431, 435, 555
BstDEI CTNAG 3 cut(s) 216, 322, 519
BstF5I GGATG 1 cut(s) 427
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 2 cut(s) 67, 559
BstKTI GATC 5 cut(s) 100, 194, 276, 354, 403
BstMAI GTCTC 3 cut(s) 4, 382, 567
BstMBI GATC 5 cut(s) 97, 191, 273, 351, 400
BstMWI GCNNNNNNNGC 2 cut(s) 44, 629
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 359
BstSLI GKGCMC 1 cut(s) 215
BstV1I GCAGC 3 cut(s) 25, 28, 31
BstXI CCANNNNNNTGG 1 cut(s) 686
BstZ17I GTATAC 1 cut(s) 692
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 7 cut(s) 72, 83, 239, 334, 618, 657, 678
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 619
BveI ACCTGC 1 cut(s) 366
Cac8I GCNNGC 5 cut(s) 117, 151, 431, 435, 555
CciI TCATGA 2 cut(s) 102, 202
CfoI GCGC 2 cut(s) 67, 559
Cfr13I GGNCC 6 cut(s) 52, 70, 82, 333, 609, 655
CsiI ACCWGGT 1 cut(s) 13
CviAII CATG 2 cut(s) 103, 203
DdeI CTNAG 3 cut(s) 216, 322, 519
DpnI GATC 5 cut(s) 99, 193, 275, 353, 402
DpnII GATC 5 cut(s) 97, 191, 273, 351, 400
DraI TTTAAA 1 cut(s) 127
EaeI YGGCCR 2 cut(s) 616, 676
Eco130I CCWWGG 2 cut(s) 234, 633
Eco31I GGTCTC 1 cut(s) 4
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 2 cut(s) 52, 609
Eco57I CTGAAG 1 cut(s) 470
EcoO109I RGGNCCY 2 cut(s) 333, 655
EcoRII CCWGG 1 cut(s) 13
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 2 cut(s) 234, 633
ErhI CCWWGG 2 cut(s) 234, 633
Esp3I CGTCTC 1 cut(s) 382
FaeI CATG 2 cut(s) 106, 206
FaiI YATR 7 cut(s) 104, 204, 209, 291, 440, 537, 692
FaqI GGGAC 1 cut(s) 65
FatI CATG 2 cut(s) 102, 202
FbaI TGATCA 1 cut(s) 351
FblI GTMKAC 2 cut(s) 228, 691
Fnu4HI GCNGC 3 cut(s) 39, 42, 45
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 3 cut(s) 39, 42, 45
FspBI CTAG 3 cut(s) 48, 647, 734
FspI TGCGCA 1 cut(s) 558
GlaI GCGC 2 cut(s) 66, 558
GluI GCNGC 3 cut(s) 39, 42, 45
GsaI CCCAGC 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 7 cut(s) 72, 83, 239, 334, 618, 657, 678
HhaI GCGC 2 cut(s) 67, 559
Hin1II CATG 2 cut(s) 106, 206
Hin6I GCGC 2 cut(s) 65, 557
HinP1I GCGC 2 cut(s) 65, 557
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 1 cut(s) 178
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 3 cut(s) 213, 229, 692
Hpy188I TCNGA 2 cut(s) 387, 418
Hpy188III TCNNGA 3 cut(s) 103, 203, 404
Hpy8I GTNNAC 3 cut(s) 213, 229, 692
Hpy99I CGWCG 1 cut(s) 387
HpyAV CCTTC 2 cut(s) 100, 445
HpyCH4III ACNGT 1 cut(s) 580
HpyCH4V TGCA 6 cut(s) 31, 213, 304, 361, 623, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 629
HpyF3I CTNAG 3 cut(s) 216, 322, 519
Hsp92II CATG 2 cut(s) 106, 206
HspAI GCGC 2 cut(s) 65, 557
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 5 cut(s) 97, 191, 273, 351, 400
LmnI GCTCC 1 cut(s) 740
Lsp1109I GCAGC 3 cut(s) 25, 28, 31
LweI GCATC 1 cut(s) 619
MabI ACCWGGT 1 cut(s) 13
MaeI CTAG 3 cut(s) 48, 647, 734
MaeIII GTNAC 2 cut(s) 278, 574
MalI GATC 5 cut(s) 99, 193, 275, 353, 402
MboI GATC 5 cut(s) 97, 191, 273, 351, 400
MboII GAAGA 2 cut(s) 152, 711
MfeI CAATTG 1 cut(s) 624
MhlI GDGCHC 1 cut(s) 215
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 6 cut(s) 134, 197, 306, 373, 543, 624
MluNI TGGCCA 1 cut(s) 678
MmeI TCCRAC 5 cut(s) 202, 365, 466, 539, 718
MnlI CCTC 7 cut(s) 250, 338, 412, 466, 486, 580, 646
Mox20I TGGCCA 1 cut(s) 678
MscI TGGCCA 1 cut(s) 678
MseI TTAA 2 cut(s) 126, 751
MslI CAYNNNNRTG 2 cut(s) 162, 684
Msp20I TGGCCA 1 cut(s) 678
MspR9I CCNGG 1 cut(s) 15
MunI CAATTG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 2 cut(s) 44, 629
NdeII GATC 5 cut(s) 97, 191, 273, 351, 400
NlaIII CATG 2 cut(s) 106, 206
NlaIV GGNNCC 2 cut(s) 53, 335
NmuCI GTSAC 2 cut(s) 278, 574
NsbI TGCGCA 1 cut(s) 558
PagI TCATGA 2 cut(s) 102, 202
PcsI WCGNNNNNNNCGW 1 cut(s) 388
PctI GAATGC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 79
PkrI GCNGC 3 cut(s) 40, 43, 46
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 53, 335
PspPI GGNCC 6 cut(s) 52, 70, 82, 333, 609, 655
PstI CTGCAG 1 cut(s) 363
RseI CAYNNNNRTG 2 cut(s) 162, 684
SaqAI TTAA 2 cut(s) 126, 751
SatI GCNGC 3 cut(s) 39, 42, 45
Sau3AI GATC 5 cut(s) 97, 191, 273, 351, 400
Sau96I GGNCC 6 cut(s) 52, 70, 82, 333, 609, 655
SbfI CCTGCAGG 1 cut(s) 363
ScrFI CCNGG 1 cut(s) 15
SdaI CCTGCAGG 1 cut(s) 363
SduI GDGCHC 1 cut(s) 215
SexAI ACCWGGT 1 cut(s) 13
SfaNI GCATC 1 cut(s) 619
SfcI CTRYAG 1 cut(s) 359
SinI GGWCC 2 cut(s) 52, 609
SmiMI CAYNNNNRTG 2 cut(s) 162, 684
SmlI CTYRAG 1 cut(s) 564
SmoI CTYRAG 1 cut(s) 564
Sse8387I CCTGCAGG 1 cut(s) 363
Sse9I AATT 6 cut(s) 134, 197, 306, 373, 543, 624
SspMI CTAG 3 cut(s) 48, 647, 734
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 2 cut(s) 234, 633
TaaI ACNGT 1 cut(s) 580
TasI AATT 6 cut(s) 134, 197, 306, 373, 543, 624
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 2 cut(s) 126, 751
Tru9I TTAA 2 cut(s) 126, 751
TscAI CASTG 1 cut(s) 619
TseFI GTSAC 2 cut(s) 278, 574
TseI GCWGC 3 cut(s) 38, 41, 44
Tsp45I GTSAC 2 cut(s) 278, 574
TspDTI ATGAA 4 cut(s) 119, 191, 306, 555
TspRI CASTG 1 cut(s) 619
Van91I CCANNNNNTGG 1 cut(s) 79
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 52, 609
XapI RAATTY 1 cut(s) 543
XmiI GTMKAC 2 cut(s) 228, 691
XspI CTAG 3 cut(s) 48, 647, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.