Rroxscaffold_1G00042080

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007352
Physical Location & Seq
Reverse (-)
60074393 .. 60077558
3166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00042080.1

Sequence Viewer

Length: 723 bp
ATGAGGAGGAGACCTGGTATTGGTGGTTTGCAGAATGCTGCTGCTGCTAGGGACCAATATCGGGCGCTGGGCCAGAATGTGGCCCAGATTAGAACTGATCTCATGAAGGAACAGCTTGCCACTTTTAAAACCCAATTAGAAGATTTTGCTCGCAAACACAAGAATGACATTCGCAAGAATCCTGCTTTTAGATCACAATTCCATGATATGTGCACTAAGGTTGGAGTAGACCCCTTGGCCTCCAATAAGGGTTTCTGGGCAGAGCTTTTGGGAATTGGTGACTTCTATTATGAACTTGGAGTGCAAATTGTTGATATCTGTTTAGCAACAAGACCCCACAATGGAGGCTTGATCAACCTGCAGGAACTCTGCAATTTGCTTCGTCGGAGACGAAAGAATGATCGTGAAGCTGTTTCCGAGGATGATTGCCTGCGTGCTATAAGTAAGCTGAAGGTGCTGGGTAATGGTTTTGAGGTTATTTCTGTTGGAAAGAGGAAGCTTGTTCGTTCTGTTCCAACTGAGTTGAACAAAGACCATAATGAAATTTTAGAGCTTGCCCAGAGAGACGGTTATCTTGGACCAGTGGCCGTGCAATTTGATGCCTTAGACACTTTACTAGATGAGGGTCTTGCAATGATTGATAATGGCCACAAAGATGGTATACGCCGATATTGGTTCCCCTGTGTATCTTCCATCTCTACCCTTGTTGGAGCTGACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
EAP30

Protein Analysis

240

Amino Acids

26.95

Weight (kDa)

8.79

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAP30 PF04157 6 - 214 1.2e-47 EAP30/Vps36 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 366
AccB7I CCANNNNNTGG 1 cut(s) 79
AccI GTMKAC 2 cut(s) 228, 661
AcoI YGGCCR 2 cut(s) 585, 646
AcsI RAATTY 1 cut(s) 543
AcuI CTGAAG 1 cut(s) 470
AfiI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
AgsI TTSAA 1 cut(s) 526
AjnI CCWGG 1 cut(s) 13
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 7 cut(s) 115, 265, 410, 448, 499, 553, 713
AluI AGCT 7 cut(s) 115, 265, 410, 448, 499, 553, 713
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 3 cut(s) 4, 382, 558
Alw44I GTGCAC 1 cut(s) 211
AoxI GGCC 5 cut(s) 70, 81, 237, 585, 646
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 3 cut(s) 38, 41, 44
ApoI RAATTY 1 cut(s) 543
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 4 cut(s) 52, 70, 82, 578
AsuHPI GGTGA 1 cut(s) 290
AvaII GGWCC 2 cut(s) 52, 578
BaeGI GKGCMC 1 cut(s) 215
BalI TGGCCA 1 cut(s) 648
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 3 cut(s) 25, 28, 31
BccI CCATC 2 cut(s) 650, 701
BceAI ACGGC 1 cut(s) 572
BciT130I CCWGG 1 cut(s) 15
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 3 cut(s) 4, 382, 558
BfaI CTAG 2 cut(s) 48, 617
BfmI CTRYAG 1 cut(s) 359
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 1 cut(s) 366
BisI GCNGC 3 cut(s) 39, 42, 45
BlsI GCNGC 3 cut(s) 40, 43, 46
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 2 cut(s) 52, 578
BmgT120I GGNCC 4 cut(s) 52, 70, 82, 578
BmiI GGNNCC 2 cut(s) 53, 677
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 589
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 2 cut(s) 234, 417
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
Bse1I ACTGG 1 cut(s) 581
Bse3DI GCAATG 1 cut(s) 639
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 234, 417
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
BseMI GCAATG 1 cut(s) 639
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 1 cut(s) 581
BseRI GAGGAG 2 cut(s) 19, 22
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 3 cut(s) 25, 28, 31
BseYI CCCAGC 2 cut(s) 67, 457
BshFI GGCC 5 cut(s) 72, 83, 239, 587, 648
BsiHKAI GWGCWC 1 cut(s) 215
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
BsmAI GTCTC 3 cut(s) 4, 382, 558
BsmBI CGTCTC 2 cut(s) 382, 558
BsmFI GGGAC 1 cut(s) 65
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 5 cut(s) 72, 83, 239, 587, 648
Bso31I GGTCTC 1 cut(s) 4
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 4 cut(s) 97, 191, 351, 400
BspANI GGCC 5 cut(s) 72, 83, 239, 587, 648
BspCNI CTCAG 1 cut(s) 511
BspHI TCATGA 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 53, 677
BspMAI CTGCAG 1 cut(s) 363
BspMI ACCTGC 1 cut(s) 366
BspTNI GGTCTC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 639
BsrI ACTGG 1 cut(s) 581
BssECI CCNNGG 2 cut(s) 234, 417
BssMI GATC 4 cut(s) 97, 191, 351, 400
BssNAI GTATAC 1 cut(s) 662
BssT1I CCWWGG 1 cut(s) 234
Bst1107I GTATAC 1 cut(s) 662
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 569
BstC8I GCNNGC 5 cut(s) 117, 151, 431, 435, 555
BstDEI CTNAG 3 cut(s) 216, 519, 604
BstF5I GGATG 1 cut(s) 427
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 4 cut(s) 100, 194, 354, 403
BstMAI GTCTC 3 cut(s) 4, 382, 558
BstMBI GATC 4 cut(s) 97, 191, 351, 400
BstMWI GCNNNNNNNGC 2 cut(s) 44, 454
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 359
BstSLI GKGCMC 1 cut(s) 215
BstV1I GCAGC 3 cut(s) 25, 28, 31
BstXI CCANNNNNNTGG 1 cut(s) 656
BstZ17I GTATAC 1 cut(s) 662
BsuRI GGCC 5 cut(s) 72, 83, 239, 587, 648
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 588
BveI ACCTGC 1 cut(s) 366
Cac8I GCNNGC 5 cut(s) 117, 151, 431, 435, 555
CciI TCATGA 1 cut(s) 102
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 4 cut(s) 52, 70, 82, 578
CsiI ACCWGGT 1 cut(s) 13
CviAII CATG 2 cut(s) 103, 203
DdeI CTNAG 3 cut(s) 216, 519, 604
DpnI GATC 4 cut(s) 99, 193, 353, 402
DpnII GATC 4 cut(s) 97, 191, 351, 400
DraI TTTAAA 1 cut(s) 127
EaeI YGGCCR 2 cut(s) 585, 646
Eco130I CCWWGG 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 4
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 2 cut(s) 52, 578
Eco57I CTGAAG 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 13
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 2 cut(s) 382, 558
FaeI CATG 2 cut(s) 106, 206
FaiI YATR 7 cut(s) 104, 204, 209, 291, 440, 537, 662
FaqI GGGAC 1 cut(s) 65
FatI CATG 2 cut(s) 102, 202
FbaI TGATCA 1 cut(s) 351
FblI GTMKAC 2 cut(s) 228, 661
Fnu4HI GCNGC 3 cut(s) 39, 42, 45
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 3 cut(s) 39, 42, 45
FspBI CTAG 2 cut(s) 48, 617
GlaI GCGC 1 cut(s) 66
GluI GCNGC 3 cut(s) 39, 42, 45
GsaI CCCAGC 2 cut(s) 71, 461
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 5 cut(s) 72, 83, 239, 587, 648
HhaI GCGC 1 cut(s) 67
Hin1II CATG 2 cut(s) 106, 206
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 1 cut(s) 178
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 3 cut(s) 213, 229, 662
Hpy188I TCNGA 2 cut(s) 387, 418
Hpy188III TCNNGA 2 cut(s) 103, 404
Hpy8I GTNNAC 3 cut(s) 213, 229, 662
Hpy99I CGWCG 1 cut(s) 387
HpyAV CCTTC 2 cut(s) 100, 445
HpyCH4III ACNGT 1 cut(s) 569
HpyCH4V TGCA 7 cut(s) 31, 213, 304, 361, 372, 592, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 454
HpyF3I CTNAG 3 cut(s) 216, 519, 604
Hsp92II CATG 2 cut(s) 106, 206
HspAI GCGC 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 4 cut(s) 97, 191, 351, 400
LmnI GCTCC 1 cut(s) 710
Lsp1109I GCAGC 3 cut(s) 25, 28, 31
LweI GCATC 1 cut(s) 589
MabI ACCWGGT 1 cut(s) 13
MaeI CTAG 2 cut(s) 48, 617
MaeIII GTNAC 1 cut(s) 278
MalI GATC 4 cut(s) 99, 193, 353, 402
MboI GATC 4 cut(s) 97, 191, 351, 400
MboII GAAGA 2 cut(s) 152, 681
MhlI GDGCHC 1 cut(s) 215
MlsI TGGCCA 1 cut(s) 648
MluCI AATT 7 cut(s) 134, 197, 273, 306, 373, 543, 593
MluNI TGGCCA 1 cut(s) 648
MmeI TCCRAC 5 cut(s) 202, 365, 466, 539, 688
MnlI CCTC 6 cut(s) 250, 338, 412, 466, 486, 616
Mox20I TGGCCA 1 cut(s) 648
MscI TGGCCA 1 cut(s) 648
MseI TTAA 2 cut(s) 126, 721
MslI CAYNNNNRTG 2 cut(s) 162, 654
Msp20I TGGCCA 1 cut(s) 648
MspR9I CCNGG 1 cut(s) 15
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 2 cut(s) 44, 454
NdeII GATC 4 cut(s) 97, 191, 351, 400
NlaIII CATG 2 cut(s) 106, 206
NlaIV GGNNCC 2 cut(s) 53, 677
NmuCI GTSAC 1 cut(s) 278
PagI TCATGA 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 388
PctI GAATGC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 79
PkrI GCNGC 3 cut(s) 40, 43, 46
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 2 cut(s) 67, 457
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 53, 677
PspPI GGNCC 4 cut(s) 52, 70, 82, 578
PstI CTGCAG 1 cut(s) 363
RseI CAYNNNNRTG 2 cut(s) 162, 654
SaqAI TTAA 2 cut(s) 126, 721
SatI GCNGC 3 cut(s) 39, 42, 45
Sau3AI GATC 4 cut(s) 97, 191, 351, 400
Sau96I GGNCC 4 cut(s) 52, 70, 82, 578
SbfI CCTGCAGG 1 cut(s) 363
ScrFI CCNGG 1 cut(s) 15
SdaI CCTGCAGG 1 cut(s) 363
SduI GDGCHC 1 cut(s) 215
SexAI ACCWGGT 1 cut(s) 13
SfaNI GCATC 1 cut(s) 589
SfcI CTRYAG 1 cut(s) 359
SinI GGWCC 2 cut(s) 52, 578
SmiMI CAYNNNNRTG 2 cut(s) 162, 654
Sse8387I CCTGCAGG 1 cut(s) 363
Sse9I AATT 7 cut(s) 134, 197, 273, 306, 373, 543, 593
SspMI CTAG 2 cut(s) 48, 617
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 569
TasI AATT 7 cut(s) 134, 197, 273, 306, 373, 543, 593
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 2 cut(s) 126, 721
Tru9I TTAA 2 cut(s) 126, 721
TscAI CASTG 1 cut(s) 588
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 3 cut(s) 38, 41, 44
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 3 cut(s) 119, 306, 555
TspRI CASTG 1 cut(s) 588
Van91I CCANNNNNTGG 1 cut(s) 79
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 52, 578
XapI RAATTY 1 cut(s) 543
XmiI GTMKAC 2 cut(s) 228, 661
XspI CTAG 2 cut(s) 48, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.