Rh5AG262600

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007352
Physical Location & Seq
Forward (+)
34504439 .. 34507974
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG262600.1

Sequence Viewer

Length: 555 bp
ATGAGGAGGAGACCTGGTATTGGTGGTTTGCAGAATGCTGCTGCTGCTAGGGACCAATATCGGGCGCTGGGCCAGAATGTGGCCCAGATTAGAACTGATCTCATGAAGGAACAGCTTGCCACTTTTAAAAGCCAATTAGAAGATTTTGCTCGCAAACACAAGGTCCAGTTTTTTTTCCAGCACTTTCAGAAGAAATTTGGTGAATCTTTTTCTTATTGGAATAAATTTGTATTGACTGTTAATATGGATGCACAGAATGACATTCGCAAGAATCCTGCTTTTAGATCACAATTTCATGATATGTGCACTAAGGTTGGAGTAGACCCCTTGGCCTCCAATAAGGGTTTCTGGGCAGAGCTTTTGGGGATCGGTGACTTCTACTATGAACTTGGAGTGCAAATTGTTGATATCTGCTTAGCAACAAGACCCCACAATGGAGGCTTGATCAACCTGCAGGAACTCTGTAATTTGCTTCGTCGGAGACGAAAAAATGATCGTGAAGCTGTTTCCGAGGATGATTGCCTGCGTGCTATAAGTAAGCTGAAGGTAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
EAP30

Protein Analysis

184

Amino Acids

21.24

Weight (kDa)

9.59

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAP30 PF04157 85 - 182 3e-29 EAP30/Vps36 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 459
AccB7I CCANNNNNTGG 1 cut(s) 79
AccI GTMKAC 1 cut(s) 321
AclWI GGATC 1 cut(s) 374
AcsI RAATTY 2 cut(s) 194, 224
AfiI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 434
AjnI CCWGG 1 cut(s) 13
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 4 cut(s) 115, 358, 503, 541
AluI AGCT 4 cut(s) 115, 358, 503, 541
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 2 cut(s) 4, 475
Alw44I GTGCAC 1 cut(s) 304
AlwI GGATC 1 cut(s) 374
AoxI GGCC 3 cut(s) 70, 81, 330
ApaLI GTGCAC 1 cut(s) 304
ApeKI GCWGC 3 cut(s) 38, 41, 44
ApoI RAATTY 2 cut(s) 194, 224
ArsI GACNNNNNNTTYG 2 cut(s) 147, 179
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 4 cut(s) 52, 70, 82, 163
AsuHPI GGTGA 2 cut(s) 212, 383
AvaII GGWCC 2 cut(s) 52, 163
BaeGI GKGCMC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 308
BbvI GCAGC 3 cut(s) 25, 28, 31
BciT130I CCWGG 1 cut(s) 15
BclI TGATCA 1 cut(s) 444
BcoDI GTCTC 2 cut(s) 4, 475
BfaI CTAG 1 cut(s) 48
BfmI CTRYAG 1 cut(s) 452
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 1 cut(s) 459
BisI GCNGC 3 cut(s) 39, 42, 45
BlpI GCTNAGC 1 cut(s) 415
BlsI GCNGC 3 cut(s) 40, 43, 46
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 2 cut(s) 52, 163
BmgT120I GGNCC 4 cut(s) 52, 70, 82, 163
BmiI GGNNCC 1 cut(s) 53
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 238
Bpu1102I GCTNAGC 1 cut(s) 415
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 2 cut(s) 327, 510
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 61, 79, 434
Bse1I ACTGG 1 cut(s) 166
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 327, 510
BseGI GGATG 2 cut(s) 253, 520
BseLI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 434
BseNI ACTGG 1 cut(s) 166
BseRI GAGGAG 2 cut(s) 19, 22
BseSI GKGCMC 1 cut(s) 308
BseXI GCAGC 3 cut(s) 25, 28, 31
BseYI CCCAGC 1 cut(s) 67
BshFI GGCC 3 cut(s) 72, 83, 332
BsiHKAI GWGCWC 1 cut(s) 308
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 434
BsmAI GTCTC 2 cut(s) 4, 475
BsmBI CGTCTC 1 cut(s) 475
BsmFI GGGAC 1 cut(s) 65
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 3 cut(s) 72, 83, 332
Bso31I GGTCTC 1 cut(s) 4
Bsp1286I GDGCHC 1 cut(s) 308
Bsp143I GATC 5 cut(s) 97, 284, 366, 444, 493
Bsp1720I GCTNAGC 1 cut(s) 415
BspANI GGCC 3 cut(s) 72, 83, 332
BspHI TCATGA 2 cut(s) 102, 295
BspLI GGNNCC 1 cut(s) 53
BspMAI CTGCAG 1 cut(s) 456
BspMI ACCTGC 1 cut(s) 459
BspPI GGATC 1 cut(s) 374
BspTNI GGTCTC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 166
BssECI CCNNGG 2 cut(s) 327, 510
BssMI GATC 5 cut(s) 97, 284, 366, 444, 493
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 238
BstC8I GCNNGC 4 cut(s) 117, 151, 524, 528
BstDEI CTNAG 2 cut(s) 309, 415
BstF5I GGATG 2 cut(s) 253, 520
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 5 cut(s) 100, 287, 369, 447, 496
BstMAI GTCTC 2 cut(s) 4, 475
BstMBI GATC 5 cut(s) 97, 284, 366, 444, 493
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 452
BstSLI GKGCMC 1 cut(s) 308
BstV1I GCAGC 3 cut(s) 25, 28, 31
BsuRI GGCC 3 cut(s) 72, 83, 332
BtsCI GGATG 2 cut(s) 253, 520
BveI ACCTGC 1 cut(s) 459
Cac8I GCNNGC 4 cut(s) 117, 151, 524, 528
CciI TCATGA 2 cut(s) 102, 295
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 4 cut(s) 52, 70, 82, 163
CsiI ACCWGGT 1 cut(s) 13
CviAII CATG 2 cut(s) 103, 296
CviJI RGCY 9 cut(s) 72, 83, 115, 132, 332, 358, 441, 503, 541
CviKI_1 RGCY 9 cut(s) 72, 83, 115, 132, 332, 358, 441, 503, 541
DdeI CTNAG 2 cut(s) 309, 415
DpnI GATC 5 cut(s) 99, 286, 368, 446, 495
DpnII GATC 5 cut(s) 97, 284, 366, 444, 493
DraI TTTAAA 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 4
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 2 cut(s) 52, 163
EcoRII CCWGG 1 cut(s) 13
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
Esp3I CGTCTC 1 cut(s) 475
FaeI CATG 2 cut(s) 106, 299
FaiI YATR 7 cut(s) 104, 245, 297, 302, 384, 533, 553
FaqI GGGAC 1 cut(s) 65
FatI CATG 2 cut(s) 102, 295
FbaI TGATCA 1 cut(s) 444
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 3 cut(s) 39, 42, 45
FokI GGATG 2 cut(s) 260, 527
Fsp4HI GCNGC 3 cut(s) 39, 42, 45
FspBI CTAG 1 cut(s) 48
GlaI GCGC 1 cut(s) 66
GluI GCNGC 3 cut(s) 39, 42, 45
GsaI CCCAGC 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 3 cut(s) 72, 83, 332
HhaI GCGC 1 cut(s) 67
Hin1II CATG 2 cut(s) 106, 299
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HinfI GANTC 2 cut(s) 203, 271
HphI GGTGA 2 cut(s) 212, 383
Hpy166II GTNNAC 2 cut(s) 306, 322
Hpy188I TCNGA 3 cut(s) 189, 480, 511
Hpy188III TCNNGA 3 cut(s) 103, 296, 497
Hpy8I GTNNAC 2 cut(s) 306, 322
Hpy99I CGWCG 1 cut(s) 480
HpyAV CCTTC 2 cut(s) 100, 538
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4V TGCA 5 cut(s) 31, 251, 306, 397, 454
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 2 cut(s) 309, 415
Hsp92II CATG 2 cut(s) 106, 299
HspAI GCGC 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 444
Kzo9I GATC 5 cut(s) 97, 284, 366, 444, 493
Lsp1109I GCAGC 3 cut(s) 25, 28, 31
LweI GCATC 1 cut(s) 238
MabI ACCWGGT 1 cut(s) 13
MaeI CTAG 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 371
MalI GATC 5 cut(s) 99, 286, 368, 446, 495
MboI GATC 5 cut(s) 97, 284, 366, 444, 493
MboII GAAGA 2 cut(s) 152, 202
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 6 cut(s) 134, 194, 224, 290, 399, 466
MmeI TCCRAC 2 cut(s) 295, 458
MnlI CCTC 3 cut(s) 343, 431, 505
MseI TTAA 2 cut(s) 126, 240
MspR9I CCNGG 1 cut(s) 15
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 44
NdeII GATC 5 cut(s) 97, 284, 366, 444, 493
NlaIII CATG 2 cut(s) 106, 299
NlaIV GGNNCC 1 cut(s) 53
NmuCI GTSAC 1 cut(s) 371
PagI TCATGA 2 cut(s) 102, 295
PcsI WCGNNNNNNNCGW 1 cut(s) 481
PctI GAATGC 1 cut(s) 40
PfeI GAWTC 2 cut(s) 203, 271
PflMI CCANNNNNTGG 1 cut(s) 79
PkrI GCNGC 3 cut(s) 40, 43, 46
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 53
PspPI GGNCC 4 cut(s) 52, 70, 82, 163
PstI CTGCAG 1 cut(s) 456
SaqAI TTAA 2 cut(s) 126, 240
SatI GCNGC 3 cut(s) 39, 42, 45
Sau3AI GATC 5 cut(s) 97, 284, 366, 444, 493
Sau96I GGNCC 4 cut(s) 52, 70, 82, 163
SbfI CCTGCAGG 1 cut(s) 456
ScrFI CCNGG 1 cut(s) 15
SdaI CCTGCAGG 1 cut(s) 456
SduI GDGCHC 1 cut(s) 308
SetI ASST 9 cut(s) 16, 117, 165, 315, 360, 453, 505, 543, 549
SexAI ACCWGGT 1 cut(s) 13
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 1 cut(s) 452
SinI GGWCC 2 cut(s) 52, 163
Sse8387I CCTGCAGG 1 cut(s) 456
Sse9I AATT 6 cut(s) 134, 194, 224, 290, 399, 466
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 238
TasI AATT 6 cut(s) 134, 194, 224, 290, 399, 466
TfiI GAWTC 2 cut(s) 203, 271
Tru1I TTAA 2 cut(s) 126, 240
Tru9I TTAA 2 cut(s) 126, 240
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 3 cut(s) 38, 41, 44
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 3 cut(s) 119, 284, 399
Van91I CCANNNNNTGG 1 cut(s) 79
VneI GTGCAC 1 cut(s) 304
VpaK11BI GGWCC 2 cut(s) 52, 163
XapI RAATTY 2 cut(s) 194, 224
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.