Prupe.4G210400_v2.0.a1

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0007352
Physical Location & Seq
Forward (+)
13120289 .. 13122652
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G210400.2

Sequence Viewer

Length: 753 bp
ATGAGGAGGCGACCTGGAATTGGCGGCTTGCAGACAGCCGCTGCTGCTAGGGACCAATATCGGTTGTTAGGCGAGAATGTCGCCAAGATCAGAACCGATCTCATGAAGGAACAGCTTACCACTTTTCGAACCCAGTTGGAAGATTTCGCTCGAAAACACAAGAATGATATTCGCAAGAACCCTGCTTTCAGATCACAATTTCATAACATGTGCACTAAGGTTGGAGTAGATCCCTTGGCCTCCAACAAGGGTTTCTGGGCAGAGCTCTTGGGGATTGGTGACTTCTATTATGAACTTGGGGTGCAAATTGTTGACATTTGCTTGGCAACAAGACCCCACAATGGAGGTTTGATCAACCTGCAAGAACTCTGCAATCTGCTTCGTCAGAGGCGAAGGAGTGACCGTGAAGCTGTGTCTGAGGATGATTGCCTGCGTGCTATAAGTAAACTGAAGGTATTGGGCAATGGTTTTGAGGTGATTTCTGTTGGAAAGCGAAAGCTTGTTCGTTCTGTTCCAACTGAGTTGAACAAAGACCATAATGAAATTTTAGAGCTAGCCCAGGCTCAAGGATTTGTGACTGTTATTGAGGTAGAGAGACGGCTATCTTGGACCACTGGTCGTGCAATTGATGCCTTCGACACTTTACTAGATGAGGGTCTTGCAATGATTGATGATGGCCACAAAGATGGTATTCGCCGATATTGGTTCCCTTGTGTATCTTCCATCTCGACTCCAGGAGGAGCTGACACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
EAP30

Protein Analysis

251

Amino Acids

28.16

Weight (kDa)

8.81

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 366
AciI CCGC 2 cut(s) 24, 39
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 1 cut(s) 543
AcuI CTGAAG 1 cut(s) 470
AfiI CCNNNNNNNGG 2 cut(s) 20, 341
AflIII ACRYGT 1 cut(s) 207
AgsI TTSAA 1 cut(s) 526
AhdI GACNNNNNGTC 1 cut(s) 615
AjnI CCWGG 3 cut(s) 13, 558, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 6 cut(s) 115, 265, 410, 499, 553, 743
AluI AGCT 6 cut(s) 115, 265, 410, 499, 553, 743
Alw21I GWGCWC 2 cut(s) 215, 267
Alw26I GTCTC 1 cut(s) 589
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 1 cut(s) 224
AlwNI CAGNNNCTG 1 cut(s) 41
AoxI GGCC 2 cut(s) 237, 676
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 2 cut(s) 41, 44
ApoI RAATTY 1 cut(s) 543
AspS9I GGNCC 2 cut(s) 52, 609
AsuHPI GGTGA 2 cut(s) 290, 487
AsuII TTCGAA 1 cut(s) 127
AsuNHI GCTAGC 1 cut(s) 553
AvaII GGWCC 2 cut(s) 52, 609
BaeGI GKGCMC 1 cut(s) 215
BalI TGGCCA 1 cut(s) 678
BanII GRGCYC 1 cut(s) 267
Bbv12I GWGCWC 2 cut(s) 215, 267
BbvI GCAGC 2 cut(s) 28, 31
BccI CCATC 3 cut(s) 668, 680, 731
BceAI ACGGC 1 cut(s) 614
BciT130I CCWGG 3 cut(s) 15, 560, 735
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 1 cut(s) 589
BfaI CTAG 3 cut(s) 48, 554, 647
BfuAI ACCTGC 1 cut(s) 366
BisI GCNGC 4 cut(s) 25, 39, 42, 45
BlsI GCNGC 4 cut(s) 26, 40, 43, 46
Bme1390I CCNGG 3 cut(s) 15, 560, 735
Bme18I GGWCC 2 cut(s) 52, 609
BmeRI GACNNNNNGTC 1 cut(s) 615
BmgT120I GGNCC 2 cut(s) 52, 609
BmiI GGNNCC 2 cut(s) 53, 707
BmrFI CCNGG 3 cut(s) 15, 560, 735
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 1 cut(s) 619
BmtI GCTAGC 1 cut(s) 557
BmuI ACTGGG 1 cut(s) 127
BpmI CTGGAG 1 cut(s) 717
Bpu14I TTCGAA 1 cut(s) 127
BpuEI CTTGAG 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 234, 558
BsaXI ACNNNNNCTCC 2 cut(s) 388, 418
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 341
Bse1I ACTGG 2 cut(s) 133, 619
Bse3DI GCAATG 2 cut(s) 469, 669
BseBI CCWGG 3 cut(s) 15, 560, 735
BseDI CCNNGG 2 cut(s) 234, 558
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 2 cut(s) 20, 341
BseMI GCAATG 2 cut(s) 469, 669
BseMII CTCAG 2 cut(s) 408, 510
BseNI ACTGG 2 cut(s) 133, 619
BseRI GAGGAG 2 cut(s) 19, 753
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 2 cut(s) 28, 31
BshFI GGCC 2 cut(s) 239, 678
BsiHKAI GWGCWC 2 cut(s) 215, 267
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 2 cut(s) 20, 341
BsmAI GTCTC 1 cut(s) 589
BsmBI CGTCTC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 65
BsnI GGCC 2 cut(s) 239, 678
Bsp119I TTCGAA 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 215, 267
Bsp143I GATC 5 cut(s) 87, 97, 191, 229, 351
BspACI CCGC 2 cut(s) 24, 39
BspANI GGCC 2 cut(s) 239, 678
BspCNI CTCAG 2 cut(s) 409, 511
BspHI TCATGA 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 53, 707
BspMI ACCTGC 1 cut(s) 366
BspOI GCTAGC 1 cut(s) 557
BspPI GGATC 1 cut(s) 224
BspT104I TTCGAA 1 cut(s) 127
BsrDI GCAATG 2 cut(s) 469, 669
BsrI ACTGG 2 cut(s) 133, 619
BssECI CCNNGG 2 cut(s) 234, 558
BssMI GATC 5 cut(s) 87, 97, 191, 229, 351
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 3 cut(s) 15, 560, 735
Bst4CI ACNGT 2 cut(s) 404, 580
BstAPI GCANNNNNTGC 1 cut(s) 629
BstBI TTCGAA 1 cut(s) 127
BstC8I GCNNGC 4 cut(s) 29, 431, 435, 555
BstDEI CTNAG 3 cut(s) 216, 417, 519
BstF5I GGATG 1 cut(s) 427
BstKTI GATC 5 cut(s) 90, 100, 194, 232, 354
BstMAI GTCTC 1 cut(s) 589
BstMBI GATC 5 cut(s) 87, 97, 191, 229, 351
BstMWI GCNNNNNNNGC 2 cut(s) 44, 629
BstNI CCWGG 3 cut(s) 15, 560, 735
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 3 cut(s) 13, 558, 733
BstSLI GKGCMC 1 cut(s) 215
BstV1I GCAGC 2 cut(s) 28, 31
BstX2I RGATCY 1 cut(s) 229
BstXI CCANNNNNNTGG 1 cut(s) 686
BstYI RGATCY 1 cut(s) 229
BsuRI GGCC 2 cut(s) 239, 678
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 612
BveI ACCTGC 1 cut(s) 366
Cac8I GCNNGC 4 cut(s) 29, 431, 435, 555
CaiI CAGNNNCTG 1 cut(s) 41
CciI TCATGA 1 cut(s) 102
Cfr13I GGNCC 2 cut(s) 52, 609
CviAII CATG 2 cut(s) 103, 208
DdeI CTNAG 3 cut(s) 216, 417, 519
DpnI GATC 5 cut(s) 89, 99, 193, 231, 353
DpnII GATC 5 cut(s) 87, 97, 191, 229, 351
DriI GACNNNNNGTC 1 cut(s) 615
EaeI YGGCCR 1 cut(s) 676
Eam1105I GACNNNNNGTC 1 cut(s) 615
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 2 cut(s) 52, 609
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 265
EcoRII CCWGG 3 cut(s) 13, 558, 733
EcoT14I CCWWGG 1 cut(s) 234
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 2 cut(s) 106, 211
FaiI YATR 6 cut(s) 104, 204, 209, 291, 440, 537
FaqI GGGAC 1 cut(s) 65
FatI CATG 2 cut(s) 102, 207
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 4 cut(s) 25, 39, 42, 45
FokI GGATG 1 cut(s) 434
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 4 cut(s) 25, 39, 42, 45
FspBI CTAG 3 cut(s) 48, 554, 647
GluI GCNGC 4 cut(s) 25, 39, 42, 45
GsuI CTGGAG 1 cut(s) 717
HaeIII GGCC 2 cut(s) 239, 678
Hin1II CATG 2 cut(s) 106, 211
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 1 cut(s) 730
HphI GGTGA 2 cut(s) 290, 487
Hpy166II GTNNAC 3 cut(s) 213, 313, 446
Hpy188I TCNGA 4 cut(s) 92, 191, 387, 418
Hpy188III TCNNGA 2 cut(s) 103, 727
Hpy8I GTNNAC 3 cut(s) 213, 313, 446
HpyAV CCTTC 4 cut(s) 100, 387, 445, 643
HpyCH4III ACNGT 2 cut(s) 404, 580
HpyCH4V TGCA 7 cut(s) 31, 213, 304, 361, 372, 623, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 629
HpyF3I CTNAG 3 cut(s) 216, 417, 519
Hsp92II CATG 2 cut(s) 106, 211
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 5 cut(s) 87, 97, 191, 229, 351
LmnI GCTCC 1 cut(s) 740
Lsp1109I GCAGC 2 cut(s) 28, 31
LweI GCATC 1 cut(s) 619
MaeI CTAG 3 cut(s) 48, 554, 647
MaeIII GTNAC 3 cut(s) 278, 398, 574
MalI GATC 5 cut(s) 89, 99, 193, 231, 353
MboI GATC 5 cut(s) 87, 97, 191, 229, 351
MboII GAAGA 2 cut(s) 152, 711
MfeI CAATTG 1 cut(s) 624
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 2 cut(s) 215, 267
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 5 cut(s) 18, 197, 306, 543, 624
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 1 cut(s) 724
MmeI TCCRAC 5 cut(s) 117, 202, 267, 466, 539
MnlI CCTC 8 cut(s) 250, 338, 381, 412, 466, 580, 646, 731
Mox20I TGGCCA 1 cut(s) 678
MscI TGGCCA 1 cut(s) 678
MslI CAYNNNNRTG 2 cut(s) 162, 684
Msp20I TGGCCA 1 cut(s) 678
MspA1I CMGCKG 1 cut(s) 41
MspR9I CCNGG 3 cut(s) 15, 560, 735
MunI CAATTG 1 cut(s) 624
MvaI CCWGG 3 cut(s) 15, 560, 735
MwoI GCNNNNNNNGC 2 cut(s) 44, 629
NdeII GATC 5 cut(s) 87, 97, 191, 229, 351
NheI GCTAGC 1 cut(s) 553
NlaIII CATG 2 cut(s) 106, 211
NlaIV GGNNCC 2 cut(s) 53, 707
NmuCI GTSAC 3 cut(s) 278, 398, 574
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 1 cut(s) 127
PagI TCATGA 1 cut(s) 102
PciI ACATGT 1 cut(s) 207
PcsI WCGNNNNNNNCGW 1 cut(s) 388
PfoI TCCNGGA 1 cut(s) 733
PkrI GCNGC 4 cut(s) 26, 40, 43, 46
PleI GAGTC 1 cut(s) 724
PpsI GAGTC 1 cut(s) 724
PscI ACATGT 1 cut(s) 207
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 3 cut(s) 13, 558, 733
PspGI CCWGG 3 cut(s) 13, 558, 733
PspN4I GGNNCC 2 cut(s) 53, 707
PspPI GGNCC 2 cut(s) 52, 609
PstNI CAGNNNCTG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 229
RseI CAYNNNNRTG 2 cut(s) 162, 684
SacI GAGCTC 1 cut(s) 267
SatI GCNGC 4 cut(s) 25, 39, 42, 45
Sau3AI GATC 5 cut(s) 87, 97, 191, 229, 351
Sau96I GGNCC 2 cut(s) 52, 609
SchI GAGTC 1 cut(s) 724
ScrFI CCNGG 3 cut(s) 15, 560, 735
SduI GDGCHC 2 cut(s) 215, 267
SfaNI GCATC 1 cut(s) 619
SfuI TTCGAA 1 cut(s) 127
SinI GGWCC 2 cut(s) 52, 609
SmiMI CAYNNNNRTG 2 cut(s) 162, 684
SmlI CTYRAG 1 cut(s) 564
SmoI CTYRAG 1 cut(s) 564
Sse9I AATT 5 cut(s) 18, 197, 306, 543, 624
SsiI CCGC 2 cut(s) 24, 39
SspMI CTAG 3 cut(s) 48, 554, 647
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 3 cut(s) 13, 558, 733
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 2 cut(s) 404, 580
TaqI TCGA 4 cut(s) 127, 151, 636, 728
TasI AATT 5 cut(s) 18, 197, 306, 543, 624
TauI GCSGC 2 cut(s) 27, 41
TscAI CASTG 1 cut(s) 619
TseFI GTSAC 3 cut(s) 278, 398, 574
TseI GCWGC 2 cut(s) 41, 44
Tsp45I GTSAC 3 cut(s) 278, 398, 574
TspDTI ATGAA 4 cut(s) 119, 191, 306, 555
TspRI CASTG 1 cut(s) 619
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 52, 609
XapI RAATTY 1 cut(s) 543
XceI RCATGY 1 cut(s) 211
XspI CTAG 3 cut(s) 48, 554, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.