Rh5DG273800

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007352
Physical Location & Seq
Forward (+)
33540345 .. 33544582
4238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG273800.1

Sequence Viewer

Length: 651 bp
ATGAAGGAACAGCTCGCCACTTTTAAAAGCCAATTAGAAGATTTTGCTCGCAAACACAAGAATGACATTCGCAAGAATCCTGCTTTTAGATCACAATTTCATGATATGTGCACTAAGGTTGGAGTAGACCCCTTGGCCTCCAATAAGGGTTTCTGGGCAGAGCTTTTGGGGATCGGTGACTTCTACTATGAACTTGGAGTGCAAATTGTTGATATCTGCTTAGCAACAAGACCCCACAATGGAGGCTTGATCAACCTGCAGGAACTCTGTAATTTGCTTCGTCGAAGACGAAAAAATGATCGTGAAGCTGTTTCCGAGGATGATTGCCTGCGTGCTATAAGTAAGCTGAAGGTATTGGGTAATGGTTTTGAGGTTATTTCTGTTGGAAAGAGGAAGCTTGTTCGTTCTGTTCCAACTGAGTTGAACAAAGACCATAATGAAATTTTAGAGCTTGCGCAGTCTCAAGGCTTTGTGACTGTTGATGAGGTAGAAAGACGGCTATCTTGGACCAGTGGCCGTGCAATTGATGCCTTGGACACTTTACTAGATGAGGGCCTTGCAATGATTGATGATGGCCACAAAGATGGTATACGCCGATATTGGTTTCCCTGTGTATCTTCCATCTCTACCCTAGTTGGAGCTGACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
EAP30

Protein Analysis

216

Amino Acids

24.39

Weight (kDa)

6.53

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAP30 PF04157 1 - 191 2.8e-53 EAP30/Vps36 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 456
Acc36I ACCTGC 1 cut(s) 264
AccI GTMKAC 2 cut(s) 126, 589
AclWI GGATC 1 cut(s) 179
AcoI YGGCCR 2 cut(s) 514, 574
AcsI RAATTY 1 cut(s) 441
AcuI CTGAAG 1 cut(s) 368
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 1 cut(s) 424
AjuI GAANNNNNNNTTGG 2 cut(s) 134, 166
AluBI AGCT 7 cut(s) 13, 163, 308, 346, 397, 451, 641
AluI AGCT 7 cut(s) 13, 163, 308, 346, 397, 451, 641
Alw21I GWGCWC 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 465
Alw44I GTGCAC 1 cut(s) 109
AlwI GGATC 1 cut(s) 179
AoxI GGCC 4 cut(s) 135, 514, 553, 574
ApaLI GTGCAC 1 cut(s) 109
ApoI RAATTY 1 cut(s) 441
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 2 cut(s) 507, 553
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 507
BaeGI GKGCMC 1 cut(s) 113
BalI TGGCCA 1 cut(s) 576
BbsI GAAGAC 1 cut(s) 292
Bbv12I GWGCWC 1 cut(s) 113
BccI CCATC 3 cut(s) 566, 578, 629
BceAI ACGGC 2 cut(s) 501, 512
BclI TGATCA 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 465
BfaI CTAG 2 cut(s) 545, 632
BfmI CTRYAG 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 264
BlpI GCTNAGC 1 cut(s) 220
Bme18I GGWCC 1 cut(s) 507
BmgT120I GGNCC 2 cut(s) 507, 553
BmsI GCATC 1 cut(s) 517
BpiI GAAGAC 1 cut(s) 292
Bpu1102I GCTNAGC 1 cut(s) 220
BpuEI CTTGAG 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 132, 315, 531
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 510
Bse3DI GCAATG 1 cut(s) 567
BseDI CCNNGG 3 cut(s) 132, 315, 531
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 1 cut(s) 510
BseSI GKGCMC 1 cut(s) 113
BshFI GGCC 4 cut(s) 137, 516, 555, 576
BsiHKAI GWGCWC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 1 cut(s) 465
BsnI GGCC 4 cut(s) 137, 516, 555, 576
Bsp1286I GDGCHC 1 cut(s) 113
Bsp143I GATC 4 cut(s) 89, 171, 249, 298
Bsp1720I GCTNAGC 1 cut(s) 220
BspANI GGCC 4 cut(s) 137, 516, 555, 576
BspCNI CTCAG 1 cut(s) 409
BspHI TCATGA 1 cut(s) 100
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 264
BspPI GGATC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 567
BsrI ACTGG 1 cut(s) 510
BssECI CCNNGG 3 cut(s) 132, 315, 531
BssMI GATC 4 cut(s) 89, 171, 249, 298
BssNAI GTATAC 1 cut(s) 590
BssT1I CCWWGG 2 cut(s) 132, 531
Bst1107I GTATAC 1 cut(s) 590
Bst4CI ACNGT 1 cut(s) 478
BstAPI GCANNNNNTGC 1 cut(s) 527
BstC8I GCNNGC 5 cut(s) 15, 49, 329, 333, 453
BstDEI CTNAG 3 cut(s) 114, 220, 417
BstF5I GGATG 1 cut(s) 325
BstHHI GCGC 1 cut(s) 457
BstKTI GATC 4 cut(s) 92, 174, 252, 301
BstMAI GTCTC 1 cut(s) 465
BstMBI GATC 4 cut(s) 89, 171, 249, 298
BstMWI GCNNNNNNNGC 1 cut(s) 527
BstSFI CTRYAG 1 cut(s) 257
BstSLI GKGCMC 1 cut(s) 113
BstV2I GAAGAC 1 cut(s) 292
BstXI CCANNNNNNTGG 1 cut(s) 584
BstZ17I GTATAC 1 cut(s) 590
BsuRI GGCC 4 cut(s) 137, 516, 555, 576
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 517
BveI ACCTGC 1 cut(s) 264
Cac8I GCNNGC 5 cut(s) 15, 49, 329, 333, 453
CciI TCATGA 1 cut(s) 100
CfoI GCGC 1 cut(s) 457
Cfr13I GGNCC 2 cut(s) 507, 553
CviAII CATG 1 cut(s) 101
DdeI CTNAG 3 cut(s) 114, 220, 417
DpnI GATC 4 cut(s) 91, 173, 251, 300
DpnII GATC 4 cut(s) 89, 171, 249, 298
DraI TTTAAA 1 cut(s) 25
EaeI YGGCCR 2 cut(s) 514, 574
Eco130I CCWWGG 2 cut(s) 132, 531
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 507
Eco57I CTGAAG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 553
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 2 cut(s) 132, 531
ErhI CCWWGG 2 cut(s) 132, 531
FaeI CATG 1 cut(s) 104
FaiI YATR 6 cut(s) 102, 107, 189, 338, 435, 590
FatI CATG 1 cut(s) 100
FbaI TGATCA 1 cut(s) 249
FblI GTMKAC 2 cut(s) 126, 589
FokI GGATG 1 cut(s) 332
FspBI CTAG 2 cut(s) 545, 632
FspI TGCGCA 1 cut(s) 456
GlaI GCGC 1 cut(s) 456
HaeIII GGCC 4 cut(s) 137, 516, 555, 576
HhaI GCGC 1 cut(s) 457
Hin1II CATG 1 cut(s) 104
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 1 cut(s) 76
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 3 cut(s) 111, 127, 590
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 2 cut(s) 101, 302
Hpy8I GTNNAC 3 cut(s) 111, 127, 590
Hpy99I CGWCG 1 cut(s) 285
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 1 cut(s) 478
HpyCH4V TGCA 5 cut(s) 111, 202, 259, 521, 560
HpyF10VI GCNNNNNNNGC 1 cut(s) 527
HpyF3I CTNAG 3 cut(s) 114, 220, 417
Hsp92II CATG 1 cut(s) 104
HspAI GCGC 1 cut(s) 455
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 4 cut(s) 89, 171, 249, 298
LmnI GCTCC 1 cut(s) 638
LpnPI CCDG 7 cut(s) 93, 139, 245, 269, 341, 523, 622
LweI GCATC 1 cut(s) 517
MaeI CTAG 2 cut(s) 545, 632
MaeIII GTNAC 2 cut(s) 176, 472
MalI GATC 4 cut(s) 91, 173, 251, 300
MboI GATC 4 cut(s) 89, 171, 249, 298
MboII GAAGA 3 cut(s) 50, 297, 609
MfeI CAATTG 1 cut(s) 522
MhlI GDGCHC 1 cut(s) 113
MlsI TGGCCA 1 cut(s) 576
MluCI AATT 6 cut(s) 32, 95, 204, 271, 441, 522
MluNI TGGCCA 1 cut(s) 576
MmeI TCCRAC 4 cut(s) 100, 364, 437, 616
MnlI CCTC 7 cut(s) 148, 236, 310, 364, 384, 478, 544
Mox20I TGGCCA 1 cut(s) 576
MscI TGGCCA 1 cut(s) 576
MseI TTAA 2 cut(s) 24, 649
MslI CAYNNNNRTG 2 cut(s) 60, 582
Msp20I TGGCCA 1 cut(s) 576
MunI CAATTG 1 cut(s) 522
MwoI GCNNNNNNNGC 1 cut(s) 527
NdeII GATC 4 cut(s) 89, 171, 249, 298
NlaIII CATG 1 cut(s) 104
NmuCI GTSAC 2 cut(s) 176, 472
NsbI TGCGCA 1 cut(s) 456
PagI TCATGA 1 cut(s) 100
PcsI WCGNNNNNNNCGW 1 cut(s) 286
PfeI GAWTC 1 cut(s) 76
PspPI GGNCC 2 cut(s) 507, 553
PstI CTGCAG 1 cut(s) 261
RseI CAYNNNNRTG 2 cut(s) 60, 582
SaqAI TTAA 2 cut(s) 24, 649
Sau3AI GATC 4 cut(s) 89, 171, 249, 298
Sau96I GGNCC 2 cut(s) 507, 553
SbfI CCTGCAGG 1 cut(s) 261
SdaI CCTGCAGG 1 cut(s) 261
SduI GDGCHC 1 cut(s) 113
SfaNI GCATC 1 cut(s) 517
SfcI CTRYAG 1 cut(s) 257
SinI GGWCC 1 cut(s) 507
SmiMI CAYNNNNRTG 2 cut(s) 60, 582
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
Sse8387I CCTGCAGG 1 cut(s) 261
Sse9I AATT 6 cut(s) 32, 95, 204, 271, 441, 522
SspMI CTAG 2 cut(s) 545, 632
StyI CCWWGG 2 cut(s) 132, 531
TaaI ACNGT 1 cut(s) 478
TaqI TCGA 1 cut(s) 283
TasI AATT 6 cut(s) 32, 95, 204, 271, 441, 522
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 2 cut(s) 24, 649
Tru9I TTAA 2 cut(s) 24, 649
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 2 cut(s) 176, 472
Tsp45I GTSAC 2 cut(s) 176, 472
TspDTI ATGAA 4 cut(s) 17, 89, 204, 453
TspRI CASTG 1 cut(s) 517
VneI GTGCAC 1 cut(s) 109
VpaK11BI GGWCC 1 cut(s) 507
XapI RAATTY 1 cut(s) 441
XmiI GTMKAC 2 cut(s) 126, 589
XspI CTAG 2 cut(s) 545, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.