MD11G1221300.v1.1

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007352
Physical Location & Seq
Reverse (-)
32415231 .. 32417332
2102 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1221300.v1.1.491

Sequence Viewer

Length: 771 bp
ATGAGGAGGAGACCTGGAATCGGCGGCTTGCAGACCGCCGCCGCCGCTAGGGACCAATATCGTCAGCTGGGCGAGAATGTTGCCAAGATCAGAACCGATCTCATGAAGGAGCAGCTCACCACTTTCCGAACCCAATTGGAAGATTTCGCTCGAAAGCACAAGAATGACATTCGAAAGAACCCAGCTTTCAGATCACAATTTCATAACATGTGCACCAAGGTAGGAGTAGATCCATTGGCATCCAATAAGGGTTTCTGGGCCGAGCTTTTGGGGATTGGTGATTTCTACTATGAACTTGGGGTGCAAATTGTTGACATTTGCTTGGCAACAAGACCCCACAATGGTGGTTTGATCAACCTGCAAGAACTTTGCGGTCTGCTTCGTCAGAGGCGGAAGAGTGATCGCGAAGCTGTTTCCGAGGATGATTGCCTCCGTGCTATAAGCAAGCTCAAGGTATTGGGTAATGGTTATGAGGTCATTTCTATTGGAAAGAGAAAGCTCGTTCGTTCCGTTCCAACTGAGTTGAACAAAGATCATAATGAAATTCTAGAGCTAGCCCAGGCTAAAGGCTTTGTGACTGTTGTTGAGGTGGAGAGACGGCTATCTTGGACCACTGGCCGTGCAATCGATGCCTTTGACACTTTATTAGATGAGGGTCTTGCAATGATTGATGATGGTCACAAAGATGGTGTCCGCCGATACTGGTTTCCCTGTGTATCCTCCATCTCCACTCCAGTAGGAGCCGACGGTTACGGAAGTTCGAAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
EAP30

Protein Analysis

257

Amino Acids

28.67

Weight (kDa)

9.07

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAP30 PF04157 6 - 225 1.6e-54 EAP30/Vps36 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 366
AccII CGCG 1 cut(s) 405
AciI CCGC 8 cut(s) 24, 36, 39, 42, 45, 372, 391, 694
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 616
AcsI RAATTY 1 cut(s) 543
AfiI CCNNNNNNNGG 3 cut(s) 20, 48, 341
AflIII ACRYGT 1 cut(s) 207
AgsI TTSAA 1 cut(s) 526
AjnI CCWGG 2 cut(s) 13, 558
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AleI CACNNNNGTG 1 cut(s) 342
AluBI AGCT 8 cut(s) 67, 115, 185, 265, 410, 448, 499, 553
AluI AGCT 8 cut(s) 67, 115, 185, 265, 410, 448, 499, 553
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 2 cut(s) 4, 589
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 1 cut(s) 224
AoxI GGCC 2 cut(s) 258, 616
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 1 cut(s) 112
ApoI RAATTY 1 cut(s) 543
ArsI GACNNNNNNTTYG 2 cut(s) 675, 707
AspS9I GGNCC 3 cut(s) 52, 258, 609
AsuHPI GGTGA 2 cut(s) 109, 290
AsuII TTCGAA 2 cut(s) 172, 761
AsuNHI GCTAGC 1 cut(s) 553
AvaII GGWCC 2 cut(s) 52, 609
BaeGI GKGCMC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 124
BccI CCATC 3 cut(s) 668, 680, 731
BceAI ACGGC 2 cut(s) 603, 614
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 2 cut(s) 15, 560
BciVI GTATCC 1 cut(s) 727
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 2 cut(s) 4, 589
BfaI CTAG 3 cut(s) 48, 548, 554
BfuAI ACCTGC 1 cut(s) 366
BfuI GTATCC 1 cut(s) 727
BisI GCNGC 5 cut(s) 25, 39, 42, 45, 113
BlsI GCNGC 5 cut(s) 26, 40, 43, 46, 114
Bme1390I CCNGG 2 cut(s) 15, 560
Bme18I GGWCC 2 cut(s) 52, 609
BmgT120I GGNCC 3 cut(s) 52, 258, 609
BmiI GGNNCC 2 cut(s) 53, 742
BmrFI CCNGG 2 cut(s) 15, 560
BmsI GCATC 2 cut(s) 248, 619
BmtI GCTAGC 1 cut(s) 557
BpmI CTGGAG 1 cut(s) 717
Bpu14I TTCGAA 2 cut(s) 172, 761
BpuEI CTTGAG 1 cut(s) 434
Bsa29I ATCGAT 1 cut(s) 627
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 3 cut(s) 216, 417, 558
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 48, 341
Bse1I ACTGG 3 cut(s) 619, 707, 734
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 2 cut(s) 15, 560
BseCI ATCGAT 1 cut(s) 627
BseDI CCNNGG 3 cut(s) 216, 417, 558
BseGI GGATG 2 cut(s) 239, 427
BseLI CCNNNNNNNGG 3 cut(s) 20, 48, 341
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 3 cut(s) 619, 707, 734
BseRI GAGGAG 2 cut(s) 19, 22
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 1 cut(s) 124
BseYI CCCAGC 2 cut(s) 67, 181
Bsh1236I CGCG 1 cut(s) 405
BshFI GGCC 2 cut(s) 260, 618
BshVI ATCGAT 1 cut(s) 627
BsiHKAI GWGCWC 1 cut(s) 215
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 3 cut(s) 20, 48, 341
BsmAI GTCTC 2 cut(s) 4, 589
BsmBI CGTCTC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 65
BsnI GGCC 2 cut(s) 260, 618
Bso31I GGTCTC 1 cut(s) 4
Bsp119I TTCGAA 2 cut(s) 172, 761
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 7 cut(s) 87, 97, 191, 229, 351, 400, 532
Bsp68I TCGCGA 1 cut(s) 405
BspACI CCGC 8 cut(s) 24, 36, 39, 42, 45, 372, 391, 694
BspANI GGCC 2 cut(s) 260, 618
BspCNI CTCAG 1 cut(s) 511
BspDI ATCGAT 1 cut(s) 627
BspFNI CGCG 1 cut(s) 405
BspHI TCATGA 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 53, 742
BspMI ACCTGC 1 cut(s) 366
BspOI GCTAGC 1 cut(s) 557
BspPI GGATC 1 cut(s) 224
BspT104I TTCGAA 2 cut(s) 172, 761
BspTNI GGTCTC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 669
BsrI ACTGG 3 cut(s) 619, 707, 734
BssECI CCNNGG 3 cut(s) 216, 417, 558
BssMI GATC 7 cut(s) 87, 97, 191, 229, 351, 400, 532
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 2 cut(s) 15, 560
Bst4CI ACNGT 2 cut(s) 580, 749
Bst6I CTCTTC 1 cut(s) 389
BstAPI GCANNNNNTGC 1 cut(s) 629
BstBI TTCGAA 2 cut(s) 172, 761
BstC8I GCNNGC 3 cut(s) 29, 446, 555
BstDEI CTNAG 1 cut(s) 519
BstF5I GGATG 2 cut(s) 239, 427
BstFNI CGCG 1 cut(s) 405
BstKTI GATC 7 cut(s) 90, 100, 194, 232, 354, 403, 535
BstMAI GTCTC 2 cut(s) 4, 589
BstMBI GATC 7 cut(s) 87, 97, 191, 229, 351, 400, 532
BstMWI GCNNNNNNNGC 2 cut(s) 44, 629
BstNI CCWGG 2 cut(s) 15, 560
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 2 cut(s) 13, 558
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 229
BstXI CCANNNNNNTGG 1 cut(s) 344
BstYI RGATCY 1 cut(s) 229
Bsu15I ATCGAT 1 cut(s) 627
BsuI GTATCC 1 cut(s) 727
BsuRI GGCC 2 cut(s) 260, 618
BsuTUI ATCGAT 1 cut(s) 627
BtsCI GGATG 2 cut(s) 239, 427
BtsIMutI CAGTG 1 cut(s) 612
BtuMI TCGCGA 1 cut(s) 405
BveI ACCTGC 1 cut(s) 366
Cac8I GCNNGC 3 cut(s) 29, 446, 555
CciI TCATGA 1 cut(s) 102
Cfr13I GGNCC 3 cut(s) 52, 258, 609
ClaI ATCGAT 1 cut(s) 627
CviAII CATG 2 cut(s) 103, 208
DdeI CTNAG 1 cut(s) 519
DpnI GATC 7 cut(s) 89, 99, 193, 231, 353, 402, 534
DpnII GATC 7 cut(s) 87, 97, 191, 229, 351, 400, 532
EaeI YGGCCR 1 cut(s) 616
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
EciI GGCGGA 2 cut(s) 406, 683
Eco130I CCWWGG 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 2 cut(s) 52, 609
EcoRII CCWGG 2 cut(s) 13, 558
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 2 cut(s) 106, 211
FaiI YATR 7 cut(s) 104, 204, 209, 291, 440, 471, 537
FaqI GGGAC 1 cut(s) 65
FatI CATG 2 cut(s) 102, 207
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 5 cut(s) 25, 39, 42, 45, 113
FokI GGATG 2 cut(s) 226, 434
Fsp4HI GCNGC 5 cut(s) 25, 39, 42, 45, 113
FspBI CTAG 3 cut(s) 48, 548, 554
GluI GCNGC 5 cut(s) 25, 39, 42, 45, 113
GsaI CCCAGC 2 cut(s) 71, 185
GsuI CTGGAG 1 cut(s) 717
HaeIII GGCC 2 cut(s) 260, 618
Hin1II CATG 2 cut(s) 106, 211
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 1 cut(s) 18
HphI GGTGA 2 cut(s) 109, 290
Hpy166II GTNNAC 2 cut(s) 213, 313
Hpy188I TCNGA 5 cut(s) 92, 128, 191, 387, 418
Hpy188III TCNNGA 3 cut(s) 103, 404, 548
Hpy8I GTNNAC 2 cut(s) 213, 313
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 2 cut(s) 100, 757
HpyCH4III ACNGT 2 cut(s) 580, 749
HpyCH4V TGCA 6 cut(s) 31, 213, 304, 361, 623, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 629
HpyF3I CTNAG 1 cut(s) 519
Hsp92II CATG 2 cut(s) 106, 211
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 7 cut(s) 87, 97, 191, 229, 351, 400, 532
LmnI GCTCC 2 cut(s) 109, 740
Lsp1109I GCAGC 1 cut(s) 124
LweI GCATC 2 cut(s) 248, 619
MaeI CTAG 3 cut(s) 48, 548, 554
MaeIII GTNAC 3 cut(s) 574, 677, 749
MalI GATC 7 cut(s) 89, 99, 193, 231, 353, 402, 534
MboI GATC 7 cut(s) 87, 97, 191, 229, 351, 400, 532
MboII GAAGA 2 cut(s) 152, 406
MfeI CAATTG 1 cut(s) 134
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 215
MluCI AATT 4 cut(s) 134, 197, 306, 543
MmeI TCCRAC 1 cut(s) 539
MnlI CCTC 7 cut(s) 381, 412, 440, 466, 580, 646, 730
MseI TTAA 1 cut(s) 769
MslI CAYNNNNRTG 3 cut(s) 162, 342, 684
MspA1I CMGCKG 1 cut(s) 67
MspR9I CCNGG 2 cut(s) 15, 560
MunI CAATTG 1 cut(s) 134
MvaI CCWGG 2 cut(s) 15, 560
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 2 cut(s) 44, 629
NdeII GATC 7 cut(s) 87, 97, 191, 229, 351, 400, 532
NheI GCTAGC 1 cut(s) 553
NlaIII CATG 2 cut(s) 106, 211
NlaIV GGNNCC 2 cut(s) 53, 742
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 2 cut(s) 574, 677
NruI TCGCGA 1 cut(s) 405
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 2 cut(s) 172, 761
OliI CACNNNNGTG 1 cut(s) 342
PagI TCATGA 1 cut(s) 102
PciI ACATGT 1 cut(s) 207
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PfeI GAWTC 1 cut(s) 18
PkrI GCNGC 5 cut(s) 26, 40, 43, 46, 114
PscI ACATGT 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 13, 558
PspFI CCCAGC 2 cut(s) 67, 181
PspGI CCWGG 2 cut(s) 13, 558
PspN4I GGNNCC 2 cut(s) 53, 742
PspPI GGNCC 3 cut(s) 52, 258, 609
PsuI RGATCY 1 cut(s) 229
PvuII CAGCTG 1 cut(s) 67
RruI TCGCGA 1 cut(s) 405
RseI CAYNNNNRTG 3 cut(s) 162, 342, 684
SaqAI TTAA 1 cut(s) 769
SatI GCNGC 5 cut(s) 25, 39, 42, 45, 113
Sau3AI GATC 7 cut(s) 87, 97, 191, 229, 351, 400, 532
Sau96I GGNCC 3 cut(s) 52, 258, 609
ScrFI CCNGG 2 cut(s) 15, 560
SduI GDGCHC 1 cut(s) 215
SfaNI GCATC 2 cut(s) 248, 619
SfuI TTCGAA 2 cut(s) 172, 761
SinI GGWCC 2 cut(s) 52, 609
SmiMI CAYNNNNRTG 3 cut(s) 162, 342, 684
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 4 cut(s) 134, 197, 306, 543
SsiI CCGC 8 cut(s) 24, 36, 39, 42, 45, 372, 391, 694
SspMI CTAG 3 cut(s) 48, 548, 554
StyD4I CCNGG 2 cut(s) 13, 558
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 2 cut(s) 580, 749
TaqI TCGA 4 cut(s) 151, 172, 627, 761
TasI AATT 4 cut(s) 134, 197, 306, 543
TauI GCSGC 4 cut(s) 27, 41, 44, 47
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 1 cut(s) 769
Tru9I TTAA 1 cut(s) 769
TscAI CASTG 1 cut(s) 619
TseFI GTSAC 2 cut(s) 574, 677
TseI GCWGC 1 cut(s) 112
Tsp45I GTSAC 2 cut(s) 574, 677
TspDTI ATGAA 4 cut(s) 119, 191, 306, 555
TspGWI ACGGA 3 cut(s) 422, 499, 768
TspRI CASTG 1 cut(s) 619
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 52, 609
XapI RAATTY 1 cut(s) 543
XbaI TCTAGA 1 cut(s) 547
XceI RCATGY 1 cut(s) 211
XspI CTAG 3 cut(s) 48, 548, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.