FvH4_3g23260

Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014695
Physical Location & Seq
Reverse (-)
16320156 .. 16322901
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23260.t1

Sequence Viewer

Length: 1362 bp
ATGGCCAAAGAGGCGAACATTGTGATGCTTGGTGCAAGTTTGATCATGATTCTCCTTCTTACAGTTGGTTTGCTTTATGCTTCATTGACCATAAGCCCCTTTGAGACATGGAAGTACAAACTGTCCAATTGGGATGATAGAAGCGTTGGAGGGGAAGAATCTCTGAGACTTCTCTTCAGAAGACTAGTTAGAGGGAAAGATCGAGTTCAGCTAGACACCACCGGGTTATCTTGCCACTTTGATCTTCACTTTGAACAGTGTTTGGCTAATAAACCAGTCATCATTGACAAGAATGCATCAACAGTTTATATACCTTCTTATGAAGCCAAATCTGAATACAAGCTTAAGCCATATGCAAGAAAAGAGGATGAGACTGCAATGAAGCTTGTCACACCGGTACGAATACTACATGGTAACATTTCTCCACCATCCTGCGATTTTATCCACCAAGTTCCGGCAGTGATCTTCTCCTCAGGTGGCTTTACAGGAAACGTATTTCATGAGTTGAATGAGATCATCATCCCCTTGTTCCTCACCTGCTACCACTTTCAATCCCGCGTTCAATTTGTCATAACTGACTTTAAGCCTTGGTGGGTGGAAAAGTACAGTCGAGTTTTATCCCAGTTGTCGAGCCATGATGTTCTAAATCCGGTTGACAATGGTAGTGTTCACTGCTTTCCAGGAGCTATATTGGGGCTCAGATATCATGATAACTTAGCTCTGAACTATACAGAAATTCCAGGAGGGTACTCCATGCTTGATTTCAAGCAATTCCTAAGAGAGTCATTTATGCTGAAAATGAAGCATGTCTCCGAGATGAATAGGCAAGAGCCGGTGCTAATGCTTCTATCTCGTCGCGGAACAAGGGAGTTTCTGAATGAAGATAAAATGGTGGAAATGATGGAAGCATTAGGCTTTCAAGTCATTGCTGCAACGCCTAATCAGACTTTAAATTTGGACACATTTTCCGGGTTGGTGAACTCGTGCAGTGTCATTGTTGGAGCTCATGGGGCTGGCCTTACAAATGCAGTGTTCTTGCCAAGCAAGGCAGTGACAGTGCAAGTAGTACCTCTGGGGTTAGATTGGGCTTCGGCTGCCTATTATGGTGAAACGGTAGCAGGTGGATTGGGGTTGGAATACTTGGAGTACAAGATTAGAGCAGAGGAAAGTTCACTTGTTGATGTCTATGGTCCGGATCACCCAGTCATTACTGATCCCATGTCTATATTCGCCAAGGGCTATGAGGCTGCCAGGGCTGTTTACGTTGATGGCCAGAATATGAAGATCAACTTGGTCAGGTTCAGGAAAACCCTTGTTGAAGCCATGAAACTTCTCGGACAGTACTCTACTCCTGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 ko00515 ko01100 map00514 map00515 map01100
Pfam Domains
Protein Families
DUF563

Protein Analysis

454

Amino Acids

50.83

Weight (kDa)

6.2

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 269 - 352 8.4e-11 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23260 FvH4_6g19650
malus_domestica MD03G1195900.v1.1
prunus_persica Prupe.4G220000_v2.0.a1
pyrus_communis pycom03g14810
rosa_chinensis RchiOBHm_Chr5g0040951
rosa_laevigata RLG00000034017
rosa_multiflora Rmu_sc0006651.1_g000006 Rmu_sc0006651.1_g000009
rosa_roxburghii Rroxscaffold_1G00039740
rosa_rugosa Rorug05G0189000 Rorug05G0189100 Rorug05G0189200
rosa_samantha Rh5BG279500 Rh5CG311700 Rh5DG287600
rosa_wichuraiana Rw5G025800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 545, 1109
Acc36I ACCTGC 2 cut(s) 545, 1109
AccII CGCG 2 cut(s) 558, 858
AccIII TCCGGA 1 cut(s) 1192
AciI CCGC 2 cut(s) 556, 858
AclWI GGATC 2 cut(s) 1203, 1208
AcoI YGGCCR 2 cut(s) 3, 1270
AcsI RAATTY 2 cut(s) 735, 952
AcuI CTGAAG 1 cut(s) 160
AfaI GTAC 7 cut(s) 116, 399, 605, 749, 1068, 1148, 1343
AfiI CCNNNNNNNGG 1 cut(s) 454
AflII CTTAAG 1 cut(s) 344
AgeI ACCGGT 1 cut(s) 394
AgsI TTSAA 7 cut(s) 254, 508, 551, 563, 766, 920, 1319
AhlI ACTAGT 1 cut(s) 184
AjnI CCWGG 3 cut(s) 679, 739, 1250
AjuI GAANNNNNNNTTGG 2 cut(s) 1274, 1306
AluBI AGCT 6 cut(s) 211, 343, 385, 686, 719, 1004
AluI AGCT 6 cut(s) 211, 343, 385, 686, 719, 1004
Alw21I GWGCWC 1 cut(s) 1006
Alw26I GTCTC 4 cut(s) 98, 160, 365, 814
AlwI GGATC 2 cut(s) 1203, 1208
Aor13HI TCCGGA 1 cut(s) 1192
AoxI GGCC 3 cut(s) 3, 1015, 1270
ApeKI GCWGC 3 cut(s) 929, 1094, 1247
ApoI RAATTY 2 cut(s) 735, 952
ArsI GACNNNNNNTTYG 2 cut(s) 937, 969
AsiGI ACCGGT 1 cut(s) 394
AspS9I GGNCC 1 cut(s) 1190
AsuC2I CCSGG 2 cut(s) 223, 970
AsuHPI GGTGA 4 cut(s) 526, 988, 1118, 1190
AvaII GGWCC 1 cut(s) 1190
AxyI CCTNAGG 1 cut(s) 472
BalI TGGCCA 2 cut(s) 5, 1272
BanII GRGCYC 2 cut(s) 699, 1006
BauI CACGAG 1 cut(s) 982
BbsI GAAGAC 1 cut(s) 187
Bbv12I GWGCWC 1 cut(s) 1006
BbvI GCAGC 3 cut(s) 916, 1081, 1234
BccI CCATC 3 cut(s) 436, 895, 1262
BciT130I CCWGG 3 cut(s) 681, 741, 1252
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 2 cut(s) 223, 970
BcoDI GTCTC 4 cut(s) 98, 160, 365, 814
BcuI ACTAGT 1 cut(s) 184
BfaI CTAG 2 cut(s) 185, 212
BfrI CTTAAG 1 cut(s) 344
BfuAI ACCTGC 2 cut(s) 545, 1109
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 3 cut(s) 930, 1095, 1248
BlsI GCNGC 3 cut(s) 931, 1096, 1249
BmcAI AGTACT 1 cut(s) 1343
Bme1390I CCNGG 5 cut(s) 223, 681, 741, 970, 1252
Bme18I GGWCC 1 cut(s) 1190
BmgT120I GGNCC 1 cut(s) 1190
BmrFI CCNGG 5 cut(s) 223, 681, 741, 970, 1252
BmrI ACTGGG 2 cut(s) 616, 1196
BmsI GCATC 2 cut(s) 15, 305
BmuI ACTGGG 2 cut(s) 616, 1196
BpiI GAAGAC 1 cut(s) 187
BpuMI CCSGG 2 cut(s) 223, 970
BsaBI GATNNNNATC 1 cut(s) 518
BsaJI CCNNGG 3 cut(s) 587, 1233, 1251
BsaWI WCCGGW 3 cut(s) 394, 649, 1192
Bsc4I CCNNNNNNNGG 1 cut(s) 454
Bse118I RCCGGY 2 cut(s) 394, 832
Bse1I ACTGG 3 cut(s) 275, 622, 1202
Bse21I CCTNAGG 1 cut(s) 472
Bse3DI GCAATG 2 cut(s) 384, 924
Bse8I GATNNNNATC 1 cut(s) 518
BseAI TCCGGA 1 cut(s) 1192
BseBI CCWGG 3 cut(s) 681, 741, 1252
BseDI CCNNGG 3 cut(s) 587, 1233, 1251
BseGI GGATG 4 cut(s) 139, 373, 428, 519
BseJI GATNNNNATC 1 cut(s) 518
BseLI CCNNNNNNNGG 1 cut(s) 454
BseMI GCAATG 2 cut(s) 384, 924
BseMII CTCAG 3 cut(s) 155, 486, 712
BseNI ACTGG 3 cut(s) 275, 622, 1202
BseRI GAGGAG 1 cut(s) 460
BseXI GCAGC 3 cut(s) 916, 1081, 1234
BsgI GTGCAG 1 cut(s) 1006
Bsh1236I CGCG 2 cut(s) 558, 858
BshFI GGCC 3 cut(s) 5, 1017, 1272
BshTI ACCGGT 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 1006
BsiSI CCGG 7 cut(s) 222, 395, 455, 650, 833, 969, 1193
BslI CCNNNNNNNGG 1 cut(s) 454
BsmAI GTCTC 4 cut(s) 98, 160, 365, 814
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 3 cut(s) 5, 1017, 1272
Bsp1286I GDGCHC 2 cut(s) 699, 1006
Bsp13I TCCGGA 1 cut(s) 1192
Bsp143I GATC 8 cut(s) 42, 199, 241, 462, 513, 1195, 1213, 1284
BspACI CCGC 2 cut(s) 556, 858
BspANI GGCC 3 cut(s) 5, 1017, 1272
BspCNI CTCAG 3 cut(s) 156, 485, 711
BspEI TCCGGA 1 cut(s) 1192
BspFNI CGCG 2 cut(s) 558, 858
BspHI TCATGA 3 cut(s) 45, 499, 706
BspMI ACCTGC 2 cut(s) 545, 1109
BspPI GGATC 2 cut(s) 1203, 1208
BspTI CTTAAG 1 cut(s) 344
BsrDI GCAATG 2 cut(s) 384, 924
BsrFI RCCGGY 2 cut(s) 394, 832
BsrI ACTGG 3 cut(s) 275, 622, 1202
BssAI RCCGGY 2 cut(s) 394, 832
BssECI CCNNGG 3 cut(s) 587, 1233, 1251
BssMI GATC 8 cut(s) 42, 199, 241, 462, 513, 1195, 1213, 1284
BssSI CACGAG 1 cut(s) 982
BssT1I CCWWGG 2 cut(s) 587, 1233
Bst2BI CACGAG 1 cut(s) 982
Bst2UI CCWGG 3 cut(s) 681, 741, 1252
Bst4CI ACNGT 8 cut(s) 64, 123, 258, 304, 608, 1057, 1114, 1341
Bst6I CTCTTC 1 cut(s) 179
BstAFI CTTAAG 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 1015
BstDEI CTNAG 5 cut(s) 164, 472, 698, 715, 776
BstF5I GGATG 4 cut(s) 139, 373, 428, 519
BstFNI CGCG 2 cut(s) 558, 858
BstKTI GATC 8 cut(s) 45, 202, 244, 465, 516, 1198, 1216, 1287
BstMAI GTCTC 4 cut(s) 98, 160, 365, 814
BstMBI GATC 8 cut(s) 42, 199, 241, 462, 513, 1195, 1213, 1284
BstMWI GCNNNNNNNGC 4 cut(s) 11, 1010, 1094, 1253
BstNI CCWGG 3 cut(s) 681, 741, 1252
BstNSI RCATGY 1 cut(s) 809
BstSCI CCNGG 5 cut(s) 221, 679, 739, 968, 1250
BstUI CGCG 2 cut(s) 558, 858
BstV1I GCAGC 3 cut(s) 916, 1081, 1234
BstV2I GAAGAC 1 cut(s) 187
Bsu36I CCTNAGG 1 cut(s) 472
BsuRI GGCC 3 cut(s) 5, 1017, 1272
BtsCI GGATG 4 cut(s) 139, 373, 428, 519
BtsI GCAGTG 5 cut(s) 465, 670, 994, 1035, 1056
BtsIMutI CAGTG 7 cut(s) 263, 465, 670, 994, 1035, 1056, 1062
BveI ACCTGC 2 cut(s) 545, 1109
Cac8I GCNNGC 1 cut(s) 1015
CciI TCATGA 3 cut(s) 45, 499, 706
Cfr10I RCCGGY 2 cut(s) 394, 832
Cfr13I GGNCC 1 cut(s) 1190
Csp6I GTAC 7 cut(s) 115, 398, 604, 748, 1067, 1147, 1342
CspAI ACCGGT 1 cut(s) 394
CviQI GTAC 7 cut(s) 115, 398, 604, 748, 1067, 1147, 1342
DdeI CTNAG 5 cut(s) 164, 472, 698, 715, 776
DpnI GATC 8 cut(s) 44, 201, 243, 464, 515, 1197, 1215, 1286
DpnII GATC 8 cut(s) 42, 199, 241, 462, 513, 1195, 1213, 1284
DraI TTTAAA 1 cut(s) 951
EaeI YGGCCR 2 cut(s) 3, 1270
Eam1104I CTCTTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 179
Ecl136II GAGCTC 1 cut(s) 1004
Eco130I CCWWGG 2 cut(s) 587, 1233
Eco24I GRGCYC 2 cut(s) 699, 1006
Eco32I GATATC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 1190
Eco53kI GAGCTC 1 cut(s) 1004
Eco57I CTGAAG 1 cut(s) 160
Eco81I CCTNAGG 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 1004
EcoRII CCWGG 3 cut(s) 679, 739, 1250
EcoRV GATATC 1 cut(s) 704
EcoT14I CCWWGG 2 cut(s) 587, 1233
EcoT22I ATGCAT 1 cut(s) 298
EcoT38I GRGCYC 2 cut(s) 699, 1006
ErhI CCWWGG 2 cut(s) 587, 1233
FalI AAGNNNNNCTT 2 cut(s) 1274, 1306
FauI CCCGC 1 cut(s) 563
FauNDI CATATG 1 cut(s) 352
FbaI TGATCA 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 930, 1095, 1248
FokI GGATG 4 cut(s) 146, 380, 415, 506
FriOI GRGCYC 2 cut(s) 699, 1006
Fsp4HI GCNGC 3 cut(s) 930, 1095, 1248
FspBI CTAG 2 cut(s) 185, 212
GluI GCNGC 3 cut(s) 930, 1095, 1248
HaeIII GGCC 3 cut(s) 5, 1017, 1272
HapII CCGG 7 cut(s) 222, 395, 455, 650, 833, 969, 1193
HincII GTYRAC 1 cut(s) 655
HindII GTYRAC 1 cut(s) 655
HindIII AAGCTT 2 cut(s) 341, 383
HinfI GANTC 3 cut(s) 49, 158, 782
HpaII CCGG 7 cut(s) 222, 395, 455, 650, 833, 969, 1193
HphI GGTGA 4 cut(s) 526, 988, 1118, 1190
Hpy166II GTNNAC 5 cut(s) 655, 670, 979, 1172, 1261
Hpy188I TCNGA 9 cut(s) 165, 179, 334, 701, 723, 814, 876, 945, 1337
Hpy188III TCNNGA 6 cut(s) 46, 500, 707, 1193, 1303, 1352
Hpy8I GTNNAC 5 cut(s) 655, 670, 979, 1172, 1261
Hpy99I CGWCG 1 cut(s) 858
HpyAV CCTTC 2 cut(s) 65, 324
HpyCH4III ACNGT 8 cut(s) 64, 123, 258, 304, 608, 1057, 1114, 1341
HpyCH4IV ACGT 2 cut(s) 492, 1263
HpyCH4V TGCA 8 cut(s) 35, 296, 356, 377, 932, 987, 1028, 1060
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 1010, 1094, 1253
HpyF3I CTNAG 5 cut(s) 164, 472, 698, 715, 776
HpySE526I ACGT 2 cut(s) 492, 1263
Kpn2I TCCGGA 1 cut(s) 1192
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 8 cut(s) 42, 199, 241, 462, 513, 1195, 1213, 1284
LmnI GCTCC 2 cut(s) 683, 1001
Lsp1109I GCAGC 3 cut(s) 916, 1081, 1234
LweI GCATC 2 cut(s) 15, 305
MaeI CTAG 2 cut(s) 185, 212
MaeII ACGT 2 cut(s) 492, 1263
MaeIII GTNAC 3 cut(s) 388, 413, 1051
MalI GATC 8 cut(s) 44, 201, 243, 464, 515, 1197, 1215, 1286
MboI GATC 8 cut(s) 42, 199, 241, 462, 513, 1195, 1213, 1284
MboII GAAGA 7 cut(s) 166, 167, 192, 236, 457, 893, 1294
MfeI CAATTG 1 cut(s) 127
MhlI GDGCHC 2 cut(s) 699, 1006
MlsI TGGCCA 2 cut(s) 5, 1272
MluCI AATT 6 cut(s) 127, 563, 735, 770, 952, 1357
MluNI TGGCCA 2 cut(s) 5, 1272
MlyI GAGTC 1 cut(s) 791
MmeI TCCRAC 3 cut(s) 127, 979, 1113
Mox20I TGGCCA 2 cut(s) 5, 1272
Mph1103I ATGCAT 1 cut(s) 298
MroI TCCGGA 1 cut(s) 1192
MscI TGGCCA 2 cut(s) 5, 1272
MseI TTAA 3 cut(s) 345, 582, 950
MslI CAYNNNNRTG 1 cut(s) 23
Msp20I TGGCCA 2 cut(s) 5, 1272
MspCI CTTAAG 1 cut(s) 344
MspI CCGG 7 cut(s) 222, 395, 455, 650, 833, 969, 1193
MspR9I CCNGG 5 cut(s) 223, 681, 741, 970, 1252
MunI CAATTG 1 cut(s) 127
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 3 cut(s) 681, 741, 1252
MvnI CGCG 2 cut(s) 558, 858
MwoI GCNNNNNNNGC 4 cut(s) 11, 1010, 1094, 1253
NciI CCSGG 2 cut(s) 223, 970
NdeI CATATG 1 cut(s) 352
NdeII GATC 8 cut(s) 42, 199, 241, 462, 513, 1195, 1213, 1284
NmuCI GTSAC 2 cut(s) 388, 1051
NsiI ATGCAT 1 cut(s) 298
NspI RCATGY 1 cut(s) 809
PagI TCATGA 3 cut(s) 45, 499, 706
PaqCI CACCTGC 2 cut(s) 545, 1109
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 49, 158
PfoI TCCNGGA 2 cut(s) 679, 739
PinAI ACCGGT 1 cut(s) 394
PkrI GCNGC 3 cut(s) 931, 1096, 1249
PleI GAGTC 1 cut(s) 790
PpsI GAGTC 1 cut(s) 790
Psp124BI GAGCTC 1 cut(s) 1006
Psp6I CCWGG 3 cut(s) 679, 739, 1250
PspGI CCWGG 3 cut(s) 679, 739, 1250
PspPI GGNCC 1 cut(s) 1190
RsaI GTAC 7 cut(s) 116, 399, 605, 749, 1068, 1148, 1343
RsaNI GTAC 7 cut(s) 115, 398, 604, 748, 1067, 1147, 1342
RseI CAYNNNNRTG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 1006
SaqAI TTAA 3 cut(s) 345, 582, 950
SatI GCNGC 3 cut(s) 930, 1095, 1248
Sau3AI GATC 8 cut(s) 42, 199, 241, 462, 513, 1195, 1213, 1284
Sau96I GGNCC 1 cut(s) 1190
ScaI AGTACT 1 cut(s) 1343
SchI GAGTC 1 cut(s) 791
ScrFI CCNGG 5 cut(s) 223, 681, 741, 970, 1252
SduI GDGCHC 2 cut(s) 699, 1006
SfaNI GCATC 2 cut(s) 15, 305
SinI GGWCC 1 cut(s) 1190
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
SpeI ACTAGT 1 cut(s) 184
Sse9I AATT 6 cut(s) 127, 563, 735, 770, 952, 1357
SsiI CCGC 2 cut(s) 556, 858
SspMI CTAG 2 cut(s) 185, 212
SstI GAGCTC 1 cut(s) 1006
StyD4I CCNGG 5 cut(s) 221, 679, 739, 968, 1250
StyI CCWWGG 2 cut(s) 587, 1233
TaaI ACNGT 8 cut(s) 64, 123, 258, 304, 608, 1057, 1114, 1341
TaiI ACGT 2 cut(s) 495, 1266
TaqI TCGA 3 cut(s) 202, 610, 629
TasI AATT 6 cut(s) 127, 563, 735, 770, 952, 1357
TatI WGTACW 4 cut(s) 114, 603, 1146, 1341
TfiI GAWTC 2 cut(s) 49, 158
Tru1I TTAA 3 cut(s) 345, 582, 950
Tru9I TTAA 3 cut(s) 345, 582, 950
TscAI CASTG 7 cut(s) 263, 465, 677, 994, 1035, 1056, 1062
TseFI GTSAC 2 cut(s) 388, 1051
TseI GCWGC 3 cut(s) 929, 1094, 1247
Tsp45I GTSAC 2 cut(s) 388, 1051
TspDTI ATGAA 9 cut(s) 72, 336, 395, 488, 815, 833, 894, 1295, 1340
TspRI CASTG 7 cut(s) 263, 465, 677, 994, 1035, 1056, 1062
Vha464I CTTAAG 1 cut(s) 344
VpaK11BI GGWCC 1 cut(s) 1190
XapI RAATTY 2 cut(s) 735, 952
XceI RCATGY 1 cut(s) 809
XspI CTAG 2 cut(s) 185, 212
ZrmI AGTACT 1 cut(s) 1343
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.