Rh5BG279500

Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0014695
Physical Location & Seq
Reverse (-)
35275477 .. 35277098
1622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG279500.1

Sequence Viewer

Length: 1359 bp
ATGGCCAAAGAATCGAACAAGGTGATGCTTGGTGCAAGTTCAATCATGATTTGCCTTCTTACCATTGGTTTGCTTTATGCTGCATTGACCATAAACCCCTTTGAGACACGGAAGCACAAGCTATCCAATTTGGATGGTAAGAGCGTTGGAGGGGAAGAACCTCTGAGATTTCTCTTCAGAAGACTGGTTAGAGGAAAAGATCGAGTTCAGCTCGATACCACTGGGTTGTCTTGCCACTCTGACCTTCACTTTGAACTGTGTTTGACTAATAAACCAGTCATAATTGACAAGAATGCATCAACAATTTATATACCATCCAAAGAAGCCAAATCTGAATACAAGGTTAAGCCATATGCAAGAAAAGAGGATGAGACTGCAATGAAGAATGTCACACCAGTAAGAATACTACATGGTAACATTACACCACCAGCCTGCGATTTTATTCACAGAGTTCCAGCAGTGATCTTCTCCTCAGGTGGCTTCACAGGAAACCTATTTCATGAGTTTAATGAGATCATCATCCCCTTGTTCCTCACCTGCCACCACTTTCGGTCCCGCATTCAATTTGTTATAACTGACTTTAAGCCTTGGTGGGTGGAAAAGTACAGTCGAGTTCTATCCCATTTGTCCAGTCATGATGTTATAAATCCGATTGAGAATGGTAGAGTTCACTGCTTTCCTGGAGCTGTCATGGGGCTGAGATATCATGACAACTTGGCTCTAAATTATACAGAAATTCCAGGAGGGTACTCTATGCTTGATTTCAAGCAATTCCTTAGAGAGTCATATATGCTGAAAATGAAGCATGTCTCGGAGATGAAAAGGCAAAGGCCGGTGCTAATGCATCTATCTCGTCGCGAAACAAGGAAGTTTCTGAATGAGGATAAAATAGTGGAAATGATGGAAGCATTAGGCTTTCAAGTCATTGTGGCAACGCCCAATCAGACATCAAATTTGGACACATTTTCCGGCTTGGTGAACTCATGCAGTGTCATTGTTGGAGCTCATGGTGCTGGCCTTACAAATGCAGTGTTCTTGCCAACCAAGGCAGTAATGGTGCAAGTGGTGCCTCTGGGGTTAGATTGGGCTTCGGCTGCCTATTATGGAGAAACGGTAGGTACTGGAATGGGGTTGGAATACTTGGAGTACAAAATTAAAGCAGAGGAAAGTTCCCTCATTGATGTCTATGGTCCTGATCACCCAGTCATTACTAATCCCGAGTCTATATTCGCGAAGGGCTATGAGGCTGCCAGGGCTGTTTATGTTGATGGCCAGAATCTCAAGATTAACTTGGTCAGGTTCAGGAAAACCCTTGTTGAAGCCATGAAACTTCTAGGACACTCTACTTCCGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 ko00515 ko01100 map00514 map00515 map01100
Pfam Domains
Protein Families
DUF563

Protein Analysis

452

Amino Acids

50.83

Weight (kDa)

8.76

Isoelectric Point (pI)

32.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 264 - 353 8.2e-12 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23260 FvH4_6g19650
malus_domestica MD03G1195900.v1.1
prunus_persica Prupe.4G220000_v2.0.a1
pyrus_communis pycom03g14810
rosa_chinensis RchiOBHm_Chr5g0040951
rosa_laevigata RLG00000034017
rosa_multiflora Rmu_sc0006651.1_g000006 Rmu_sc0006651.1_g000009
rosa_roxburghii Rroxscaffold_1G00039740
rosa_rugosa Rorug05G0189000 Rorug05G0189100 Rorug05G0189200
rosa_samantha Rh5BG279500 Rh5CG311700 Rh5DG287600
rosa_wichuraiana Rw5G025800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 572, 644
AarI CACCTGC 1 cut(s) 545
Acc36I ACCTGC 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 1066
AccII CGCG 2 cut(s) 858, 1232
AciI CCGC 1 cut(s) 556
AcoI YGGCCR 2 cut(s) 3, 1270
AcsI RAATTY 2 cut(s) 735, 952
AcuI CTGAAG 1 cut(s) 160
AfaI GTAC 4 cut(s) 605, 749, 1120, 1148
AgsI TTSAA 6 cut(s) 42, 254, 563, 766, 920, 1319
AjnI CCWGG 3 cut(s) 679, 739, 1250
AluBI AGCT 4 cut(s) 121, 211, 686, 1004
AluI AGCT 4 cut(s) 121, 211, 686, 1004
Alw21I GWGCWC 1 cut(s) 1006
Alw26I GTCTC 3 cut(s) 98, 365, 814
Ama87I CYCGRG 1 cut(s) 1217
AoxI GGCC 4 cut(s) 3, 830, 1015, 1270
ApeKI GCWGC 3 cut(s) 80, 1094, 1247
ApoI RAATTY 2 cut(s) 735, 952
ArsI GACNNNNNNTTYG 4 cut(s) 233, 265, 937, 969
AspS9I GGNCC 2 cut(s) 552, 1190
AsuHPI GGTGA 4 cut(s) 34, 526, 988, 1190
AvaI CYCGRG 1 cut(s) 1217
AvaII GGWCC 2 cut(s) 552, 1190
AxyI CCTNAGG 1 cut(s) 472
BalI TGGCCA 2 cut(s) 5, 1272
BanI GGYRCC 1 cut(s) 1066
BanII GRGCYC 1 cut(s) 1006
BbsI GAAGAC 1 cut(s) 187
Bbv12I GWGCWC 1 cut(s) 1006
BbvI GCAGC 3 cut(s) 67, 1081, 1234
BccI CCATC 4 cut(s) 128, 322, 895, 1262
BcgI CGANNNNNNTGC 2 cut(s) 833, 867
BciT130I CCWGG 3 cut(s) 681, 741, 1252
BclI TGATCA 1 cut(s) 1195
BcoDI GTCTC 3 cut(s) 98, 365, 814
BfaI CTAG 1 cut(s) 1334
BfuAI ACCTGC 1 cut(s) 545
BisI GCNGC 3 cut(s) 81, 1095, 1248
BlsI GCNGC 3 cut(s) 82, 1096, 1249
Bme1390I CCNGG 3 cut(s) 681, 741, 1252
Bme18I GGWCC 2 cut(s) 552, 1190
BmeT110I CYCGRG 1 cut(s) 1217
BmgT120I GGNCC 2 cut(s) 552, 1190
BmiI GGNNCC 2 cut(s) 554, 1068
BmrFI CCNGG 3 cut(s) 681, 741, 1252
BmrI ACTGGG 2 cut(s) 231, 1196
BmsI GCATC 3 cut(s) 15, 305, 853
BmuI ACTGGG 2 cut(s) 231, 1196
BpiI GAAGAC 1 cut(s) 187
BplI GAGNNNNNCTC 2 cut(s) 195, 227
BpmI CTGGAG 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 1265
BsaBI GATNNNNATC 1 cut(s) 518
BsaJI CCNNGG 3 cut(s) 587, 1044, 1251
BsaXI ACNNNNNCTCC 2 cut(s) 1098, 1128
Bse118I RCCGGY 1 cut(s) 832
Bse1I ACTGG 7 cut(s) 189, 226, 275, 395, 630, 1126, 1202
Bse21I CCTNAGG 1 cut(s) 472
Bse3DI GCAATG 1 cut(s) 384
Bse8I GATNNNNATC 1 cut(s) 518
BseBI CCWGG 3 cut(s) 681, 741, 1252
BseDI CCNNGG 3 cut(s) 587, 1044, 1251
BseGI GGATG 4 cut(s) 139, 314, 373, 519
BseJI GATNNNNATC 1 cut(s) 518
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 3 cut(s) 155, 486, 689
BseNI ACTGG 7 cut(s) 189, 226, 275, 395, 630, 1126, 1202
BseRI GAGGAG 1 cut(s) 460
BseXI GCAGC 3 cut(s) 67, 1081, 1234
Bsh1236I CGCG 2 cut(s) 858, 1232
BshFI GGCC 4 cut(s) 5, 832, 1017, 1272
BshNI GGYRCC 1 cut(s) 1066
BsiHKAI GWGCWC 1 cut(s) 1006
BsiHKCI CYCGRG 1 cut(s) 1217
BsiSI CCGG 2 cut(s) 833, 969
BslFI GGGAC 1 cut(s) 538
BsmAI GTCTC 3 cut(s) 98, 365, 814
BsmFI GGGAC 1 cut(s) 538
BsmI GAATGC 2 cut(s) 298, 558
BsnI GGCC 4 cut(s) 5, 832, 1017, 1272
BsoBI CYCGRG 1 cut(s) 1217
Bsp1286I GDGCHC 1 cut(s) 1006
Bsp143I GATC 4 cut(s) 199, 462, 513, 1195
Bsp68I TCGCGA 2 cut(s) 858, 1232
BspACI CCGC 1 cut(s) 556
BspANI GGCC 4 cut(s) 5, 832, 1017, 1272
BspCNI CTCAG 3 cut(s) 156, 485, 690
BspFNI CGCG 2 cut(s) 858, 1232
BspHI TCATGA 4 cut(s) 45, 499, 634, 706
BspLI GGNNCC 2 cut(s) 554, 1068
BspMI ACCTGC 1 cut(s) 545
BspT107I GGYRCC 1 cut(s) 1066
BsrDI GCAATG 1 cut(s) 384
BsrFI RCCGGY 1 cut(s) 832
BsrI ACTGG 7 cut(s) 189, 226, 275, 395, 630, 1126, 1202
BssAI RCCGGY 1 cut(s) 832
BssECI CCNNGG 3 cut(s) 587, 1044, 1251
BssMI GATC 4 cut(s) 199, 462, 513, 1195
BssT1I CCWWGG 2 cut(s) 587, 1044
Bst2UI CCWGG 3 cut(s) 681, 741, 1252
Bst4CI ACNGT 3 cut(s) 258, 608, 1114
Bst6I CTCTTC 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 1066
BstC8I GCNNGC 2 cut(s) 433, 1015
BstDEI CTNAG 4 cut(s) 164, 472, 698, 776
BstF5I GGATG 4 cut(s) 139, 314, 373, 519
BstFNI CGCG 2 cut(s) 858, 1232
BstKTI GATC 4 cut(s) 202, 465, 516, 1198
BstMAI GTCTC 3 cut(s) 98, 365, 814
BstMBI GATC 4 cut(s) 199, 462, 513, 1195
BstMWI GCNNNNNNNGC 4 cut(s) 1010, 1066, 1094, 1253
BstNI CCWGG 3 cut(s) 681, 741, 1252
BstNSI RCATGY 1 cut(s) 809
BstSCI CCNGG 3 cut(s) 679, 739, 1250
BstUI CGCG 2 cut(s) 858, 1232
BstV1I GCAGC 3 cut(s) 67, 1081, 1234
BstV2I GAAGAC 1 cut(s) 187
Bsu36I CCTNAGG 1 cut(s) 472
BsuRI GGCC 4 cut(s) 5, 832, 1017, 1272
BtsCI GGATG 4 cut(s) 139, 314, 373, 519
BtsI GCAGTG 4 cut(s) 465, 670, 994, 1035
BtsIMutI CAGTG 5 cut(s) 219, 465, 670, 994, 1035
BtuMI TCGCGA 2 cut(s) 858, 1232
BveI ACCTGC 1 cut(s) 545
Cac8I GCNNGC 2 cut(s) 433, 1015
CciI TCATGA 4 cut(s) 45, 499, 634, 706
Cfr10I RCCGGY 1 cut(s) 832
Cfr13I GGNCC 2 cut(s) 552, 1190
Csp6I GTAC 4 cut(s) 604, 748, 1119, 1147
CviQI GTAC 4 cut(s) 604, 748, 1119, 1147
DdeI CTNAG 4 cut(s) 164, 472, 698, 776
DpnI GATC 4 cut(s) 201, 464, 515, 1197
DpnII GATC 4 cut(s) 199, 462, 513, 1195
EaeI YGGCCR 2 cut(s) 3, 1270
Eam1104I CTCTTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 179
Ecl136II GAGCTC 1 cut(s) 1004
Eco130I CCWWGG 2 cut(s) 587, 1044
Eco24I GRGCYC 1 cut(s) 1006
Eco32I GATATC 1 cut(s) 704
Eco47I GGWCC 2 cut(s) 552, 1190
Eco53kI GAGCTC 1 cut(s) 1004
Eco57I CTGAAG 1 cut(s) 160
Eco81I CCTNAGG 1 cut(s) 472
Eco88I CYCGRG 1 cut(s) 1217
EcoICRI GAGCTC 1 cut(s) 1004
EcoRII CCWGG 3 cut(s) 679, 739, 1250
EcoRV GATATC 1 cut(s) 704
EcoT14I CCWWGG 2 cut(s) 587, 1044
EcoT22I ATGCAT 2 cut(s) 298, 846
EcoT38I GRGCYC 1 cut(s) 1006
ErhI CCWWGG 2 cut(s) 587, 1044
FalI AAGNNNNNCTT 2 cut(s) 1274, 1306
FaqI GGGAC 1 cut(s) 538
FauI CCCGC 1 cut(s) 563
FauNDI CATATG 1 cut(s) 352
FbaI TGATCA 1 cut(s) 1195
Fnu4HI GCNGC 3 cut(s) 81, 1095, 1248
FokI GGATG 4 cut(s) 146, 301, 380, 506
FriOI GRGCYC 1 cut(s) 1006
Fsp4HI GCNGC 3 cut(s) 81, 1095, 1248
FspBI CTAG 1 cut(s) 1334
GluI GCNGC 3 cut(s) 81, 1095, 1248
GsuI CTGGAG 1 cut(s) 702
HaeIII GGCC 4 cut(s) 5, 832, 1017, 1272
HapII CCGG 2 cut(s) 833, 969
HinfI GANTC 4 cut(s) 11, 782, 1220, 1276
HpaII CCGG 2 cut(s) 833, 969
HphI GGTGA 4 cut(s) 34, 526, 988, 1190
Hpy166II GTNNAC 2 cut(s) 670, 979
Hpy188I TCNGA 9 cut(s) 165, 179, 241, 334, 651, 814, 876, 945, 1351
Hpy8I GTNNAC 2 cut(s) 670, 979
Hpy99I CGWCG 1 cut(s) 858
HpyAV CCTTC 3 cut(s) 65, 254, 1228
HpyCH4III ACNGT 3 cut(s) 258, 608, 1114
HpyCH4V TGCA 9 cut(s) 35, 83, 296, 356, 377, 844, 987, 1028, 1060
HpyF10VI GCNNNNNNNGC 4 cut(s) 1010, 1066, 1094, 1253
HpyF3I CTNAG 4 cut(s) 164, 472, 698, 776
Ksp22I TGATCA 1 cut(s) 1195
Kzo9I GATC 4 cut(s) 199, 462, 513, 1195
LmnI GCTCC 2 cut(s) 683, 1001
Lsp1109I GCAGC 3 cut(s) 67, 1081, 1234
LweI GCATC 3 cut(s) 15, 305, 853
MaeI CTAG 1 cut(s) 1334
MaeIII GTNAC 2 cut(s) 388, 413
MalI GATC 4 cut(s) 201, 464, 515, 1197
MboI GATC 4 cut(s) 199, 462, 513, 1195
MboII GAAGA 5 cut(s) 166, 167, 192, 394, 457
MhlI GDGCHC 1 cut(s) 1006
MlsI TGGCCA 2 cut(s) 5, 1272
MluNI TGGCCA 2 cut(s) 5, 1272
MlyI GAGTC 2 cut(s) 791, 1229
MmeI TCCRAC 3 cut(s) 127, 979, 1113
Mox20I TGGCCA 2 cut(s) 5, 1272
Mph1103I ATGCAT 2 cut(s) 298, 846
MscI TGGCCA 2 cut(s) 5, 1272
MseI TTAA 5 cut(s) 345, 507, 582, 1155, 1287
Msp20I TGGCCA 2 cut(s) 5, 1272
MspI CCGG 2 cut(s) 833, 969
MspR9I CCNGG 3 cut(s) 681, 741, 1252
Mva1269I GAATGC 2 cut(s) 298, 558
MvaI CCWGG 3 cut(s) 681, 741, 1252
MvnI CGCG 2 cut(s) 858, 1232
MwoI GCNNNNNNNGC 4 cut(s) 1010, 1066, 1094, 1253
NdeI CATATG 1 cut(s) 352
NdeII GATC 4 cut(s) 199, 462, 513, 1195
NlaIV GGNNCC 2 cut(s) 554, 1068
NmuCI GTSAC 1 cut(s) 388
NruI TCGCGA 2 cut(s) 858, 1232
NsiI ATGCAT 2 cut(s) 298, 846
NspI RCATGY 1 cut(s) 809
PagI TCATGA 4 cut(s) 45, 499, 634, 706
PaqCI CACCTGC 1 cut(s) 545
PctI GAATGC 2 cut(s) 298, 558
PfeI GAWTC 2 cut(s) 11, 1276
PfoI TCCNGGA 2 cut(s) 679, 739
PkrI GCNGC 3 cut(s) 82, 1096, 1249
PleI GAGTC 2 cut(s) 790, 1228
PpsI GAGTC 2 cut(s) 790, 1228
PsiI TTATAA 2 cut(s) 572, 644
Psp124BI GAGCTC 1 cut(s) 1006
Psp6I CCWGG 3 cut(s) 679, 739, 1250
PspGI CCWGG 3 cut(s) 679, 739, 1250
PspN4I GGNNCC 2 cut(s) 554, 1068
PspPI GGNCC 2 cut(s) 552, 1190
RruI TCGCGA 2 cut(s) 858, 1232
RsaI GTAC 4 cut(s) 605, 749, 1120, 1148
RsaNI GTAC 4 cut(s) 604, 748, 1119, 1147
SacI GAGCTC 1 cut(s) 1006
SaqAI TTAA 5 cut(s) 345, 507, 582, 1155, 1287
SatI GCNGC 3 cut(s) 81, 1095, 1248
Sau3AI GATC 4 cut(s) 199, 462, 513, 1195
Sau96I GGNCC 2 cut(s) 552, 1190
SchI GAGTC 2 cut(s) 791, 1229
ScrFI CCNGG 3 cut(s) 681, 741, 1252
SduI GDGCHC 1 cut(s) 1006
SfaNI GCATC 3 cut(s) 15, 305, 853
SinI GGWCC 2 cut(s) 552, 1190
SmlI CTYRAG 1 cut(s) 1280
SmoI CTYRAG 1 cut(s) 1280
SsiI CCGC 1 cut(s) 556
SspMI CTAG 1 cut(s) 1334
SstI GAGCTC 1 cut(s) 1006
StyD4I CCNGG 3 cut(s) 679, 739, 1250
StyI CCWWGG 2 cut(s) 587, 1044
TaaI ACNGT 3 cut(s) 258, 608, 1114
TaqI TCGA 4 cut(s) 14, 202, 213, 610
TaqII GACCGA 1 cut(s) 540
TatI WGTACW 2 cut(s) 603, 1146
TfiI GAWTC 2 cut(s) 11, 1276
Tru1I TTAA 5 cut(s) 345, 507, 582, 1155, 1287
Tru9I TTAA 5 cut(s) 345, 507, 582, 1155, 1287
TscAI CASTG 5 cut(s) 226, 465, 677, 994, 1035
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 3 cut(s) 80, 1094, 1247
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 5 cut(s) 395, 488, 815, 833, 1340
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 5 cut(s) 226, 465, 677, 994, 1035
VpaK11BI GGWCC 2 cut(s) 552, 1190
XapI RAATTY 2 cut(s) 735, 952
XceI RCATGY 1 cut(s) 809
XcmI CCANNNNNNNNNTGG 1 cut(s) 1051
XspI CTAG 1 cut(s) 1334
Zsp2I ATGCAT 2 cut(s) 298, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.