RchiOBHm_Chr5g0040951

Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014695
Physical Location & Seq
Reverse (-)
35596885 .. 35598063
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31942

Sequence Viewer

Length: 1179 bp
ATGCATACTGCAGGAAAAGATCGAGTTCAGCTCGATACCACTGGGTTGTCTTGCCACTCTGACCTTCACTTTGAACTGTGTTTGACTAATAAACCAGTCATAATTGACAAGAATGCATCAACAGTTTATATACCTTCCAAAGAAGCCAAATCTGAATACAAGGTTAAGCCATATGCAAGAAAAGAGGATGAGACTGCAATGAAGATTGTCACACCAGTAAGAATACTACATGGTAACATTACACCACCAGCCTGCGATTTTATTCACAGAGTTCCAGCAGTGATCTTCTCCTCAGGTGGCTTCACAGGAAACCTATTTCATGAGTTTAATGAGATCATCATCCCCTTGTTCCTCACCTGCCACCACTTTCGATCCCGCATTCAATTTGTTATAACTGACTTTAAGCCTTGGTGGGTGGAAAAGTACAGTCGAGTTCTATCCCATTTGTCCAGTCATGATGTTATAAATCCGGTTGAGAATGGTAGTGTTCACTGCTTTCCAGGAGCTGTCATGGGGCTGAGATATCATGACAACTTGGCTCTAAATTATACAGAAATTCCAGGAGGGTACTCTATGCTTGATTTCAAGCAATTCCTTAGAGAGTCATATATGCTGAAAATGAAGCATGTCTCGGAGATGAAAAGGCAAAGGCCGGTGCTAATTCATCTCTCTCGTCGCGAAACAAGGAAGTTTCTGAATGAAGATAAAATAGTGGAAATGATGGAAGCATTAGGCTTTCAAGTCATTGTGGCAACGCCCAATCAGACATCAAATTTGGACACATTTTCCGGCTTGGTGAACTCATGCAGTGTCATTGTTGGAGCTCATGGTGCTGGCCTTACAAATGCAGTGTTCTTGCCAACCAAGGCAGTAATGGTGCAAGTGGTGCCTCTGGGGTTAGATTGGGCTTCGGCTGCCTATTATGGAGAAACGGTAGGTACTGGAATGGGGTTGGAATACTTGGAGTACAAAATTAAAGCAGAGGAAAGTTCCCTCATTGATGTCTATGGTCCTGATCACCCAGTCATTACTGATCCCGAGTCTATATTCGCGAAGGGCTATGAGGCTGCCAGGGCTGTTTATGTTGATGGCCAGAATCTCAAGATTAACTTGGTCAGGTTCAGGAAAACCCTTGTTGAAGCCATGAAACTTCTAGGACACTCTACTTCCGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 ko00515 ko01100 map00514 map00515 map01100
Pfam Domains
Protein Families
DUF563

Protein Analysis

392

Amino Acids

44.03

Weight (kDa)

6.99

Isoelectric Point (pI)

29.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 202 - 293 2.6e-12 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23260 FvH4_6g19650
malus_domestica MD03G1195900.v1.1
prunus_persica Prupe.4G220000_v2.0.a1
pyrus_communis pycom03g14810
rosa_chinensis RchiOBHm_Chr5g0040951
rosa_laevigata RLG00000034017
rosa_multiflora Rmu_sc0006651.1_g000006 Rmu_sc0006651.1_g000009
rosa_roxburghii Rroxscaffold_1G00039740
rosa_rugosa Rorug05G0189000 Rorug05G0189100 Rorug05G0189200
rosa_samantha Rh5BG279500 Rh5CG311700 Rh5DG287600
rosa_wichuraiana Rw5G025800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 392, 464
AarI CACCTGC 1 cut(s) 365
Acc36I ACCTGC 1 cut(s) 365
AccB1I GGYRCC 1 cut(s) 886
AccII CGCG 2 cut(s) 678, 1052
AciI CCGC 1 cut(s) 376
AclWI GGATC 2 cut(s) 366, 1028
AcoI YGGCCR 1 cut(s) 1090
AcsI RAATTY 2 cut(s) 555, 772
AfaI GTAC 4 cut(s) 425, 569, 940, 968
AgsI TTSAA 5 cut(s) 74, 383, 586, 740, 1139
AjnI CCWGG 3 cut(s) 499, 559, 1070
AluBI AGCT 3 cut(s) 31, 506, 824
AluI AGCT 3 cut(s) 31, 506, 824
Alw21I GWGCWC 1 cut(s) 826
Alw26I GTCTC 2 cut(s) 185, 634
AlwI GGATC 2 cut(s) 366, 1028
AlwNI CAGNNNCTG 1 cut(s) 506
Ama87I CYCGRG 1 cut(s) 1037
AoxI GGCC 3 cut(s) 650, 835, 1090
ApeKI GCWGC 2 cut(s) 914, 1067
ApoI RAATTY 2 cut(s) 555, 772
ArsI GACNNNNNNTTYG 4 cut(s) 53, 85, 757, 789
AspS9I GGNCC 1 cut(s) 1010
AsuHPI GGTGA 3 cut(s) 346, 808, 1010
AvaI CYCGRG 1 cut(s) 1037
AvaII GGWCC 1 cut(s) 1010
AxyI CCTNAGG 1 cut(s) 292
BalI TGGCCA 1 cut(s) 1092
BanI GGYRCC 1 cut(s) 886
BanII GRGCYC 1 cut(s) 826
Bbv12I GWGCWC 1 cut(s) 826
BbvI GCAGC 2 cut(s) 901, 1054
BccI CCATC 2 cut(s) 715, 1082
BciT130I CCWGG 3 cut(s) 501, 561, 1072
BclI TGATCA 1 cut(s) 1015
BcoDI GTCTC 2 cut(s) 185, 634
BfaI CTAG 1 cut(s) 1154
BfmI CTRYAG 1 cut(s) 9
BfuAI ACCTGC 1 cut(s) 365
BisI GCNGC 2 cut(s) 915, 1068
BlsI GCNGC 2 cut(s) 916, 1069
Bme1390I CCNGG 3 cut(s) 501, 561, 1072
Bme18I GGWCC 1 cut(s) 1010
BmeT110I CYCGRG 1 cut(s) 1037
BmgT120I GGNCC 1 cut(s) 1010
BmiI GGNNCC 1 cut(s) 888
BmrFI CCNGG 3 cut(s) 501, 561, 1072
BmrI ACTGGG 2 cut(s) 51, 1016
BmsI GCATC 1 cut(s) 125
BmuI ACTGGG 2 cut(s) 51, 1016
BplI GAGNNNNNCTC 2 cut(s) 15, 47
BpuEI CTTGAG 1 cut(s) 1085
BsaBI GATNNNNATC 1 cut(s) 338
BsaJI CCNNGG 3 cut(s) 407, 864, 1071
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 2 cut(s) 918, 948
Bse118I RCCGGY 1 cut(s) 652
Bse1I ACTGG 6 cut(s) 46, 95, 215, 450, 946, 1022
Bse21I CCTNAGG 1 cut(s) 292
Bse3DI GCAATG 1 cut(s) 204
Bse8I GATNNNNATC 1 cut(s) 338
BseBI CCWGG 3 cut(s) 501, 561, 1072
BseDI CCNNGG 3 cut(s) 407, 864, 1071
BseGI GGATG 2 cut(s) 193, 339
BseJI GATNNNNATC 1 cut(s) 338
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 2 cut(s) 306, 509
BseNI ACTGG 6 cut(s) 46, 95, 215, 450, 946, 1022
BseRI GAGGAG 1 cut(s) 280
BseXI GCAGC 2 cut(s) 901, 1054
Bsh1236I CGCG 2 cut(s) 678, 1052
BshFI GGCC 3 cut(s) 652, 837, 1092
BshNI GGYRCC 1 cut(s) 886
BsiHKAI GWGCWC 1 cut(s) 826
BsiHKCI CYCGRG 1 cut(s) 1037
BsiSI CCGG 3 cut(s) 470, 653, 789
BsmAI GTCTC 2 cut(s) 185, 634
BsmI GAATGC 2 cut(s) 118, 378
BsnI GGCC 3 cut(s) 652, 837, 1092
BsoBI CYCGRG 1 cut(s) 1037
Bsp1286I GDGCHC 1 cut(s) 826
Bsp143I GATC 6 cut(s) 19, 282, 333, 371, 1015, 1033
Bsp68I TCGCGA 2 cut(s) 678, 1052
BspACI CCGC 1 cut(s) 376
BspANI GGCC 3 cut(s) 652, 837, 1092
BspCNI CTCAG 2 cut(s) 305, 510
BspFNI CGCG 2 cut(s) 678, 1052
BspHI TCATGA 3 cut(s) 319, 454, 526
BspLI GGNNCC 1 cut(s) 888
BspMAI CTGCAG 1 cut(s) 13
BspMI ACCTGC 1 cut(s) 365
BspPI GGATC 2 cut(s) 366, 1028
BspT107I GGYRCC 1 cut(s) 886
BsrDI GCAATG 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 652
BsrI ACTGG 6 cut(s) 46, 95, 215, 450, 946, 1022
BssAI RCCGGY 1 cut(s) 652
BssECI CCNNGG 3 cut(s) 407, 864, 1071
BssMI GATC 6 cut(s) 19, 282, 333, 371, 1015, 1033
BssT1I CCWWGG 2 cut(s) 407, 864
Bst2UI CCWGG 3 cut(s) 501, 561, 1072
Bst4CI ACNGT 4 cut(s) 78, 124, 428, 934
BstAPI GCANNNNNTGC 1 cut(s) 886
BstC8I GCNNGC 2 cut(s) 253, 835
BstDEI CTNAG 3 cut(s) 292, 518, 596
BstF5I GGATG 2 cut(s) 193, 339
BstFNI CGCG 2 cut(s) 678, 1052
BstKTI GATC 6 cut(s) 22, 285, 336, 374, 1018, 1036
BstMAI GTCTC 2 cut(s) 185, 634
BstMBI GATC 6 cut(s) 19, 282, 333, 371, 1015, 1033
BstMWI GCNNNNNNNGC 4 cut(s) 830, 886, 914, 1073
BstNI CCWGG 3 cut(s) 501, 561, 1072
BstNSI RCATGY 1 cut(s) 629
BstSCI CCNGG 3 cut(s) 499, 559, 1070
BstSFI CTRYAG 1 cut(s) 9
BstUI CGCG 2 cut(s) 678, 1052
BstV1I GCAGC 2 cut(s) 901, 1054
Bsu36I CCTNAGG 1 cut(s) 292
BsuRI GGCC 3 cut(s) 652, 837, 1092
BtsCI GGATG 2 cut(s) 193, 339
BtsI GCAGTG 4 cut(s) 285, 490, 814, 855
BtsIMutI CAGTG 5 cut(s) 39, 285, 490, 814, 855
BtuMI TCGCGA 2 cut(s) 678, 1052
BveI ACCTGC 1 cut(s) 365
Cac8I GCNNGC 2 cut(s) 253, 835
CaiI CAGNNNCTG 1 cut(s) 506
CciI TCATGA 3 cut(s) 319, 454, 526
Cfr10I RCCGGY 1 cut(s) 652
Cfr13I GGNCC 1 cut(s) 1010
Csp6I GTAC 4 cut(s) 424, 568, 939, 967
CviAII CATG 9 cut(s) 230, 320, 455, 511, 527, 626, 804, 827, 1144
CviQI GTAC 4 cut(s) 424, 568, 939, 967
DdeI CTNAG 3 cut(s) 292, 518, 596
DpnI GATC 6 cut(s) 21, 284, 335, 373, 1017, 1035
DpnII GATC 6 cut(s) 19, 282, 333, 371, 1015, 1033
EaeI YGGCCR 1 cut(s) 1090
Ecl136II GAGCTC 1 cut(s) 824
Eco130I CCWWGG 2 cut(s) 407, 864
Eco24I GRGCYC 1 cut(s) 826
Eco32I GATATC 1 cut(s) 524
Eco47I GGWCC 1 cut(s) 1010
Eco53kI GAGCTC 1 cut(s) 824
Eco81I CCTNAGG 1 cut(s) 292
Eco88I CYCGRG 1 cut(s) 1037
EcoICRI GAGCTC 1 cut(s) 824
EcoRII CCWGG 3 cut(s) 499, 559, 1070
EcoRV GATATC 1 cut(s) 524
EcoT14I CCWWGG 2 cut(s) 407, 864
EcoT22I ATGCAT 2 cut(s) 6, 118
EcoT38I GRGCYC 1 cut(s) 826
ErhI CCWWGG 2 cut(s) 407, 864
FaeI CATG 9 cut(s) 233, 323, 458, 514, 530, 629, 807, 830, 1147
FalI AAGNNNNNCTT 2 cut(s) 1094, 1126
FatI CATG 9 cut(s) 229, 319, 454, 510, 526, 625, 803, 826, 1143
FauI CCCGC 1 cut(s) 383
FauNDI CATATG 1 cut(s) 172
FbaI TGATCA 1 cut(s) 1015
Fnu4HI GCNGC 2 cut(s) 915, 1068
FokI GGATG 2 cut(s) 200, 326
FriOI GRGCYC 1 cut(s) 826
Fsp4HI GCNGC 2 cut(s) 915, 1068
FspBI CTAG 1 cut(s) 1154
GluI GCNGC 2 cut(s) 915, 1068
HaeIII GGCC 3 cut(s) 652, 837, 1092
HapII CCGG 3 cut(s) 470, 653, 789
Hin1II CATG 9 cut(s) 233, 323, 458, 514, 530, 629, 807, 830, 1147
HinfI GANTC 3 cut(s) 602, 1040, 1096
HpaII CCGG 3 cut(s) 470, 653, 789
HphI GGTGA 3 cut(s) 346, 808, 1010
Hpy166II GTNNAC 2 cut(s) 490, 799
Hpy188I TCNGA 6 cut(s) 61, 154, 634, 696, 765, 1171
Hpy188III TCNNGA 9 cut(s) 320, 455, 527, 677, 1013, 1037, 1051, 1102, 1123
Hpy8I GTNNAC 2 cut(s) 490, 799
Hpy99I CGWCG 1 cut(s) 678
HpyAV CCTTC 3 cut(s) 74, 144, 1048
HpyCH4III ACNGT 4 cut(s) 78, 124, 428, 934
HpyCH4V TGCA 8 cut(s) 4, 11, 116, 176, 197, 807, 848, 880
HpyF10VI GCNNNNNNNGC 4 cut(s) 830, 886, 914, 1073
HpyF3I CTNAG 3 cut(s) 292, 518, 596
Hsp92II CATG 9 cut(s) 233, 323, 458, 514, 530, 629, 807, 830, 1147
Ksp22I TGATCA 1 cut(s) 1015
Kzo9I GATC 6 cut(s) 19, 282, 333, 371, 1015, 1033
LmnI GCTCC 2 cut(s) 503, 821
Lsp1109I GCAGC 2 cut(s) 901, 1054
LweI GCATC 1 cut(s) 125
MaeI CTAG 1 cut(s) 1154
MaeIII GTNAC 2 cut(s) 208, 233
MalI GATC 6 cut(s) 21, 284, 335, 373, 1017, 1035
MboI GATC 6 cut(s) 19, 282, 333, 371, 1015, 1033
MboII GAAGA 3 cut(s) 214, 277, 713
MhlI GDGCHC 1 cut(s) 826
MlsI TGGCCA 1 cut(s) 1092
MluCI AATT 9 cut(s) 102, 383, 544, 555, 590, 660, 772, 972, 1174
MluNI TGGCCA 1 cut(s) 1092
MlyI GAGTC 2 cut(s) 611, 1049
MmeI TCCRAC 2 cut(s) 799, 933
MnlI CCTC 8 cut(s) 178, 301, 362, 557, 900, 976, 1004, 1057
Mox20I TGGCCA 1 cut(s) 1092
Mph1103I ATGCAT 2 cut(s) 6, 118
MscI TGGCCA 1 cut(s) 1092
MseI TTAA 5 cut(s) 165, 327, 402, 975, 1107
Msp20I TGGCCA 1 cut(s) 1092
MspI CCGG 3 cut(s) 470, 653, 789
MspR9I CCNGG 3 cut(s) 501, 561, 1072
Mva1269I GAATGC 2 cut(s) 118, 378
MvaI CCWGG 3 cut(s) 501, 561, 1072
MvnI CGCG 2 cut(s) 678, 1052
MwoI GCNNNNNNNGC 4 cut(s) 830, 886, 914, 1073
NdeI CATATG 1 cut(s) 172
NdeII GATC 6 cut(s) 19, 282, 333, 371, 1015, 1033
NlaIII CATG 9 cut(s) 233, 323, 458, 514, 530, 629, 807, 830, 1147
NlaIV GGNNCC 1 cut(s) 888
NmuCI GTSAC 1 cut(s) 208
NruI TCGCGA 2 cut(s) 678, 1052
NsiI ATGCAT 2 cut(s) 6, 118
NspI RCATGY 1 cut(s) 629
PagI TCATGA 3 cut(s) 319, 454, 526
PaqCI CACCTGC 1 cut(s) 365
PctI GAATGC 2 cut(s) 118, 378
PfeI GAWTC 1 cut(s) 1096
PfoI TCCNGGA 2 cut(s) 499, 559
PkrI GCNGC 2 cut(s) 916, 1069
PleI GAGTC 2 cut(s) 610, 1048
PpsI GAGTC 2 cut(s) 610, 1048
PsiI TTATAA 2 cut(s) 392, 464
Psp124BI GAGCTC 1 cut(s) 826
Psp6I CCWGG 3 cut(s) 499, 559, 1070
PspGI CCWGG 3 cut(s) 499, 559, 1070
PspN4I GGNNCC 1 cut(s) 888
PspPI GGNCC 1 cut(s) 1010
PstI CTGCAG 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 506
RruI TCGCGA 2 cut(s) 678, 1052
RsaI GTAC 4 cut(s) 425, 569, 940, 968
RsaNI GTAC 4 cut(s) 424, 568, 939, 967
SacI GAGCTC 1 cut(s) 826
SaqAI TTAA 5 cut(s) 165, 327, 402, 975, 1107
SatI GCNGC 2 cut(s) 915, 1068
Sau3AI GATC 6 cut(s) 19, 282, 333, 371, 1015, 1033
Sau96I GGNCC 1 cut(s) 1010
SchI GAGTC 2 cut(s) 611, 1049
ScrFI CCNGG 3 cut(s) 501, 561, 1072
SduI GDGCHC 1 cut(s) 826
SfaNI GCATC 1 cut(s) 125
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 1010
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
Sse9I AATT 9 cut(s) 102, 383, 544, 555, 590, 660, 772, 972, 1174
SsiI CCGC 1 cut(s) 376
SspMI CTAG 1 cut(s) 1154
SstI GAGCTC 1 cut(s) 826
StyD4I CCNGG 3 cut(s) 499, 559, 1070
StyI CCWWGG 2 cut(s) 407, 864
TaaI ACNGT 4 cut(s) 78, 124, 428, 934
TaqI TCGA 4 cut(s) 22, 33, 370, 430
TasI AATT 9 cut(s) 102, 383, 544, 555, 590, 660, 772, 972, 1174
TatI WGTACW 2 cut(s) 423, 966
TfiI GAWTC 1 cut(s) 1096
Tru1I TTAA 5 cut(s) 165, 327, 402, 975, 1107
Tru9I TTAA 5 cut(s) 165, 327, 402, 975, 1107
TscAI CASTG 5 cut(s) 46, 285, 497, 814, 855
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 2 cut(s) 914, 1067
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 7 cut(s) 215, 308, 635, 653, 653, 714, 1160
TspRI CASTG 5 cut(s) 46, 285, 497, 814, 855
VpaK11BI GGWCC 1 cut(s) 1010
XapI RAATTY 2 cut(s) 555, 772
XceI RCATGY 1 cut(s) 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 871
XspI CTAG 1 cut(s) 1154
Zsp2I ATGCAT 2 cut(s) 6, 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.