Rw5G025800

Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014695
Physical Location & Seq
Reverse (-)
36914057 .. 36915673
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025800.1

Sequence Viewer

Length: 1287 bp
ATGGCCAAAGAATCGAACAAGGTGATGCTTGGTGCAAGTTCAATCATGATTTGCCTTCTTACCATTGGTTTGCTTTATGCTGCATTGACCATAAACCCCTTTGAGACACGGAAGCACAAGCTATCCAATTTGGATGGTAAGAGCGTTGGAGGGGAAGAACCTCTGAGATTTTCTTCAGAAGACTGGTTAGAGCTCGATACCACTGGGTTGTCTTGCCACTCTGACCTTCACTTTGAACTGTGTTTGACTAATAAACCAGTCATAATTGACAAGAATGCATCAACAGTTTATATACCTTCCAAAGAAGCCAAATCTGAATACAAGGTTAAGCCATATGCAAGAAAAGAGGATGAGACTGCAATGAAGATGTCACACCAAGTTCCAGCAGTGATCTTCTCCTCAGGTGGCTTCACAGGAAACCTATTTCATGAGTTTAATGAGATCATCATCCCCTTGTTCCTCACCTGCCACCACTTTCGATCCCGCATTCAATTTGTTATAACTGACTTTAAGCCTTGGTGGGTGGAAAAGTACAGTCGAGTTCTATCCCATTTGTCCAGTCATGATGTTATAAATCCGGTTGAGAATGGTAGTGTTCACTGCTTTCCTGGAGCTGTCATGGGGCTGAGATATCATGACAACTTGGCTCTAAATTATACAGAAATTCCAGGAGGGTACTCTATGCTTGATTTCAAGCAATTCCTTAGAGAGTCATATATGCTGAAAATGAAGCATGTCTCGGAGATGAAAAGGCAAAGGCCGGTGCTAATGCATCTATCTCGTCGCGAAACAAGGAAGTTTCTGAATGAAGATAAAATAGTGGAAATGATGGAAGCATTAGGCTTTCAAGTCATTGTGGCAACGCCCAATCAGACATCAAATTTGGATACATTTTCCGGCTTGGTGAACTCATGCAGTGTCATTGTTGGAGCTCATGGTGCTGGCCTTACAAATGCAGTGTTCTTGCCAACCAAGGCAGTAATGGTGCAAGTGGTGCCTCTGGGGTTAGATTGGGCTTCGGCTGCCTATTATGGAGAAACGGTAGGTACTGGAATGGGGTTGGAATACTTGGAGTACAAAATTAAAGCAGAGGAAAGTTCCCTCATTGATGTCTATGGTCCTGATCACCCAGTCATTACTGATCCCGAGTCTATATTCGCGAAGGGCTATGAGGCTGCCAGGGCTGTTTATGTTGATGGCCAGAATCTCAAGATTAACTTGGTCAGGTTCAGGAAAACCCTTGTTGAAGCCATGAAACTTCTAGGACACTCTACTTCCGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 ko00515 ko01100 map00514 map00515 map01100
Pfam Domains
Protein Families
DUF563

Protein Analysis

428

Amino Acids

47.99

Weight (kDa)

6.47

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 240 - 329 8e-12 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23260 FvH4_6g19650
malus_domestica MD03G1195900.v1.1
prunus_persica Prupe.4G220000_v2.0.a1
pyrus_communis pycom03g14810
rosa_chinensis RchiOBHm_Chr5g0040951
rosa_laevigata RLG00000034017
rosa_multiflora Rmu_sc0006651.1_g000006 Rmu_sc0006651.1_g000009
rosa_roxburghii Rroxscaffold_1G00039740
rosa_rugosa Rorug05G0189000 Rorug05G0189100 Rorug05G0189200
rosa_samantha Rh5BG279500 Rh5CG311700 Rh5DG287600
rosa_wichuraiana Rw5G025800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 500, 572
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 1 cut(s) 473
AccB1I GGYRCC 1 cut(s) 994
AccII CGCG 2 cut(s) 786, 1160
AciI CCGC 1 cut(s) 484
AclWI GGATC 2 cut(s) 474, 1136
AcoI YGGCCR 2 cut(s) 3, 1198
AcsI RAATTY 2 cut(s) 663, 880
AcuI CTGAAG 1 cut(s) 159
AfaI GTAC 4 cut(s) 533, 677, 1048, 1076
AgsI TTSAA 6 cut(s) 42, 236, 491, 694, 848, 1247
AjnI CCWGG 3 cut(s) 607, 667, 1178
AluBI AGCT 4 cut(s) 121, 193, 614, 932
AluI AGCT 4 cut(s) 121, 193, 614, 932
Alw21I GWGCWC 2 cut(s) 195, 934
Alw26I GTCTC 3 cut(s) 98, 347, 742
AlwI GGATC 2 cut(s) 474, 1136
Ama87I CYCGRG 1 cut(s) 1145
AoxI GGCC 4 cut(s) 3, 758, 943, 1198
ApeKI GCWGC 3 cut(s) 80, 1022, 1175
ApoI RAATTY 2 cut(s) 663, 880
ArsI GACNNNNNNTTYG 4 cut(s) 215, 247, 865, 897
AspS9I GGNCC 1 cut(s) 1118
AsuHPI GGTGA 4 cut(s) 34, 454, 916, 1118
AvaI CYCGRG 1 cut(s) 1145
AvaII GGWCC 1 cut(s) 1118
AxyI CCTNAGG 1 cut(s) 400
BalI TGGCCA 2 cut(s) 5, 1200
BanI GGYRCC 1 cut(s) 994
BanII GRGCYC 2 cut(s) 195, 934
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 2 cut(s) 195, 934
BbvI GCAGC 3 cut(s) 67, 1009, 1162
BccI CCATC 3 cut(s) 128, 823, 1190
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciT130I CCWGG 3 cut(s) 609, 669, 1180
BciVI GTATCC 1 cut(s) 880
BclI TGATCA 1 cut(s) 1123
BcoDI GTCTC 3 cut(s) 98, 347, 742
BfaI CTAG 1 cut(s) 1262
BfuAI ACCTGC 1 cut(s) 473
BfuI GTATCC 1 cut(s) 880
BisI GCNGC 3 cut(s) 81, 1023, 1176
BlsI GCNGC 3 cut(s) 82, 1024, 1177
Bme1390I CCNGG 3 cut(s) 609, 669, 1180
Bme18I GGWCC 1 cut(s) 1118
BmeT110I CYCGRG 1 cut(s) 1145
BmgT120I GGNCC 1 cut(s) 1118
BmiI GGNNCC 1 cut(s) 996
BmrFI CCNGG 3 cut(s) 609, 669, 1180
BmrI ACTGGG 2 cut(s) 213, 1124
BmsI GCATC 3 cut(s) 15, 287, 781
BmuI ACTGGG 2 cut(s) 213, 1124
BpiI GAAGAC 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 630
BpuEI CTTGAG 1 cut(s) 1193
BsaBI GATNNNNATC 1 cut(s) 446
BsaJI CCNNGG 3 cut(s) 515, 972, 1179
BsaWI WCCGGW 1 cut(s) 577
BsaXI ACNNNNNCTCC 2 cut(s) 1026, 1056
Bse118I RCCGGY 1 cut(s) 760
Bse1I ACTGG 6 cut(s) 188, 208, 257, 558, 1054, 1130
Bse21I CCTNAGG 1 cut(s) 400
Bse3DI GCAATG 1 cut(s) 366
Bse8I GATNNNNATC 1 cut(s) 446
BseBI CCWGG 3 cut(s) 609, 669, 1180
BseDI CCNNGG 3 cut(s) 515, 972, 1179
BseGI GGATG 3 cut(s) 139, 355, 447
BseJI GATNNNNATC 1 cut(s) 446
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 3 cut(s) 155, 414, 617
BseNI ACTGG 6 cut(s) 188, 208, 257, 558, 1054, 1130
BseRI GAGGAG 1 cut(s) 388
BseXI GCAGC 3 cut(s) 67, 1009, 1162
Bsh1236I CGCG 2 cut(s) 786, 1160
BshFI GGCC 4 cut(s) 5, 760, 945, 1200
BshNI GGYRCC 1 cut(s) 994
BsiHKAI GWGCWC 2 cut(s) 195, 934
BsiHKCI CYCGRG 1 cut(s) 1145
BsiSI CCGG 3 cut(s) 578, 761, 897
BsmAI GTCTC 3 cut(s) 98, 347, 742
BsmI GAATGC 2 cut(s) 280, 486
BsnI GGCC 4 cut(s) 5, 760, 945, 1200
BsoBI CYCGRG 1 cut(s) 1145
Bsp1286I GDGCHC 2 cut(s) 195, 934
Bsp143I GATC 5 cut(s) 390, 441, 479, 1123, 1141
Bsp68I TCGCGA 2 cut(s) 786, 1160
BspACI CCGC 1 cut(s) 484
BspANI GGCC 4 cut(s) 5, 760, 945, 1200
BspCNI CTCAG 3 cut(s) 156, 413, 618
BspFNI CGCG 2 cut(s) 786, 1160
BspHI TCATGA 4 cut(s) 45, 427, 562, 634
BspLI GGNNCC 1 cut(s) 996
BspMI ACCTGC 1 cut(s) 473
BspPI GGATC 2 cut(s) 474, 1136
BspT107I GGYRCC 1 cut(s) 994
BsrDI GCAATG 1 cut(s) 366
BsrFI RCCGGY 1 cut(s) 760
BsrI ACTGG 6 cut(s) 188, 208, 257, 558, 1054, 1130
BssAI RCCGGY 1 cut(s) 760
BssECI CCNNGG 3 cut(s) 515, 972, 1179
BssMI GATC 5 cut(s) 390, 441, 479, 1123, 1141
BssT1I CCWWGG 2 cut(s) 515, 972
Bst2UI CCWGG 3 cut(s) 609, 669, 1180
Bst4CI ACNGT 4 cut(s) 240, 286, 536, 1042
BstAPI GCANNNNNTGC 1 cut(s) 994
BstC8I GCNNGC 1 cut(s) 943
BstDEI CTNAG 4 cut(s) 164, 400, 626, 704
BstF5I GGATG 3 cut(s) 139, 355, 447
BstFNI CGCG 2 cut(s) 786, 1160
BstKTI GATC 5 cut(s) 393, 444, 482, 1126, 1144
BstMAI GTCTC 3 cut(s) 98, 347, 742
BstMBI GATC 5 cut(s) 390, 441, 479, 1123, 1141
BstMWI GCNNNNNNNGC 4 cut(s) 938, 994, 1022, 1181
BstNI CCWGG 3 cut(s) 609, 669, 1180
BstNSI RCATGY 1 cut(s) 737
BstSCI CCNGG 3 cut(s) 607, 667, 1178
BstUI CGCG 2 cut(s) 786, 1160
BstV1I GCAGC 3 cut(s) 67, 1009, 1162
BstV2I GAAGAC 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 400
BsuI GTATCC 1 cut(s) 880
BsuRI GGCC 4 cut(s) 5, 760, 945, 1200
BtsCI GGATG 3 cut(s) 139, 355, 447
BtsI GCAGTG 4 cut(s) 393, 598, 922, 963
BtsIMutI CAGTG 5 cut(s) 201, 393, 598, 922, 963
BtuMI TCGCGA 2 cut(s) 786, 1160
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 1 cut(s) 943
CciI TCATGA 4 cut(s) 45, 427, 562, 634
Cfr10I RCCGGY 1 cut(s) 760
Cfr13I GGNCC 1 cut(s) 1118
Csp6I GTAC 4 cut(s) 532, 676, 1047, 1075
CviAII CATG 9 cut(s) 46, 428, 563, 619, 635, 734, 912, 935, 1252
CviQI GTAC 4 cut(s) 532, 676, 1047, 1075
DdeI CTNAG 4 cut(s) 164, 400, 626, 704
DpnI GATC 5 cut(s) 392, 443, 481, 1125, 1143
DpnII GATC 5 cut(s) 390, 441, 479, 1123, 1141
EaeI YGGCCR 2 cut(s) 3, 1198
Ecl136II GAGCTC 2 cut(s) 193, 932
Eco130I CCWWGG 2 cut(s) 515, 972
Eco24I GRGCYC 2 cut(s) 195, 934
Eco32I GATATC 1 cut(s) 632
Eco47I GGWCC 1 cut(s) 1118
Eco53kI GAGCTC 2 cut(s) 193, 932
Eco57I CTGAAG 1 cut(s) 159
Eco81I CCTNAGG 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 1145
EcoICRI GAGCTC 2 cut(s) 193, 932
EcoRII CCWGG 3 cut(s) 607, 667, 1178
EcoRV GATATC 1 cut(s) 632
EcoT14I CCWWGG 2 cut(s) 515, 972
EcoT22I ATGCAT 2 cut(s) 280, 774
EcoT38I GRGCYC 2 cut(s) 195, 934
ErhI CCWWGG 2 cut(s) 515, 972
FaeI CATG 9 cut(s) 49, 431, 566, 622, 638, 737, 915, 938, 1255
FalI AAGNNNNNCTT 2 cut(s) 1202, 1234
FatI CATG 9 cut(s) 45, 427, 562, 618, 634, 733, 911, 934, 1251
FauI CCCGC 1 cut(s) 491
FauNDI CATATG 1 cut(s) 334
FbaI TGATCA 1 cut(s) 1123
Fnu4HI GCNGC 3 cut(s) 81, 1023, 1176
FokI GGATG 3 cut(s) 146, 362, 434
FriOI GRGCYC 2 cut(s) 195, 934
Fsp4HI GCNGC 3 cut(s) 81, 1023, 1176
FspBI CTAG 1 cut(s) 1262
GluI GCNGC 3 cut(s) 81, 1023, 1176
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 4 cut(s) 5, 760, 945, 1200
HapII CCGG 3 cut(s) 578, 761, 897
Hin1II CATG 9 cut(s) 49, 431, 566, 622, 638, 737, 915, 938, 1255
HinfI GANTC 4 cut(s) 11, 710, 1148, 1204
HpaII CCGG 3 cut(s) 578, 761, 897
HphI GGTGA 4 cut(s) 34, 454, 916, 1118
Hpy166II GTNNAC 2 cut(s) 598, 907
Hpy188I TCNGA 8 cut(s) 165, 178, 223, 316, 742, 804, 873, 1279
Hpy8I GTNNAC 2 cut(s) 598, 907
Hpy99I CGWCG 1 cut(s) 786
HpyAV CCTTC 4 cut(s) 65, 236, 306, 1156
HpyCH4III ACNGT 4 cut(s) 240, 286, 536, 1042
HpyCH4V TGCA 9 cut(s) 35, 83, 278, 338, 359, 772, 915, 956, 988
HpyF10VI GCNNNNNNNGC 4 cut(s) 938, 994, 1022, 1181
HpyF3I CTNAG 4 cut(s) 164, 400, 626, 704
Hsp92II CATG 9 cut(s) 49, 431, 566, 622, 638, 737, 915, 938, 1255
Ksp22I TGATCA 1 cut(s) 1123
Kzo9I GATC 5 cut(s) 390, 441, 479, 1123, 1141
LmnI GCTCC 2 cut(s) 611, 929
Lsp1109I GCAGC 3 cut(s) 67, 1009, 1162
LweI GCATC 3 cut(s) 15, 287, 781
MaeI CTAG 1 cut(s) 1262
MaeIII GTNAC 1 cut(s) 369
MalI GATC 5 cut(s) 392, 443, 481, 1125, 1143
MboI GATC 5 cut(s) 390, 441, 479, 1123, 1141
MboII GAAGA 6 cut(s) 165, 167, 191, 376, 385, 821
MhlI GDGCHC 2 cut(s) 195, 934
MlsI TGGCCA 2 cut(s) 5, 1200
MluCI AATT 9 cut(s) 127, 264, 491, 652, 663, 698, 880, 1080, 1282
MluNI TGGCCA 2 cut(s) 5, 1200
MlyI GAGTC 2 cut(s) 719, 1157
MmeI TCCRAC 3 cut(s) 127, 907, 1041
Mox20I TGGCCA 2 cut(s) 5, 1200
Mph1103I ATGCAT 2 cut(s) 280, 774
MscI TGGCCA 2 cut(s) 5, 1200
MseI TTAA 5 cut(s) 327, 435, 510, 1083, 1215
Msp20I TGGCCA 2 cut(s) 5, 1200
MspI CCGG 3 cut(s) 578, 761, 897
MspR9I CCNGG 3 cut(s) 609, 669, 1180
Mva1269I GAATGC 2 cut(s) 280, 486
MvaI CCWGG 3 cut(s) 609, 669, 1180
MvnI CGCG 2 cut(s) 786, 1160
MwoI GCNNNNNNNGC 4 cut(s) 938, 994, 1022, 1181
NdeI CATATG 1 cut(s) 334
NdeII GATC 5 cut(s) 390, 441, 479, 1123, 1141
NlaIII CATG 9 cut(s) 49, 431, 566, 622, 638, 737, 915, 938, 1255
NlaIV GGNNCC 1 cut(s) 996
NmuCI GTSAC 1 cut(s) 369
NruI TCGCGA 2 cut(s) 786, 1160
NsiI ATGCAT 2 cut(s) 280, 774
NspI RCATGY 1 cut(s) 737
PagI TCATGA 4 cut(s) 45, 427, 562, 634
PaqCI CACCTGC 1 cut(s) 473
PctI GAATGC 2 cut(s) 280, 486
PfeI GAWTC 2 cut(s) 11, 1204
PfoI TCCNGGA 2 cut(s) 607, 667
PkrI GCNGC 3 cut(s) 82, 1024, 1177
PleI GAGTC 2 cut(s) 718, 1156
PpsI GAGTC 2 cut(s) 718, 1156
PsiI TTATAA 2 cut(s) 500, 572
Psp124BI GAGCTC 2 cut(s) 195, 934
Psp6I CCWGG 3 cut(s) 607, 667, 1178
PspGI CCWGG 3 cut(s) 607, 667, 1178
PspN4I GGNNCC 1 cut(s) 996
PspPI GGNCC 1 cut(s) 1118
RruI TCGCGA 2 cut(s) 786, 1160
RsaI GTAC 4 cut(s) 533, 677, 1048, 1076
RsaNI GTAC 4 cut(s) 532, 676, 1047, 1075
SacI GAGCTC 2 cut(s) 195, 934
SaqAI TTAA 5 cut(s) 327, 435, 510, 1083, 1215
SatI GCNGC 3 cut(s) 81, 1023, 1176
Sau3AI GATC 5 cut(s) 390, 441, 479, 1123, 1141
Sau96I GGNCC 1 cut(s) 1118
SchI GAGTC 2 cut(s) 719, 1157
ScrFI CCNGG 3 cut(s) 609, 669, 1180
SduI GDGCHC 2 cut(s) 195, 934
SfaNI GCATC 3 cut(s) 15, 287, 781
SinI GGWCC 1 cut(s) 1118
SmlI CTYRAG 1 cut(s) 1208
SmoI CTYRAG 1 cut(s) 1208
Sse9I AATT 9 cut(s) 127, 264, 491, 652, 663, 698, 880, 1080, 1282
SsiI CCGC 1 cut(s) 484
SspMI CTAG 1 cut(s) 1262
SstI GAGCTC 2 cut(s) 195, 934
StyD4I CCNGG 3 cut(s) 607, 667, 1178
StyI CCWWGG 2 cut(s) 515, 972
TaaI ACNGT 4 cut(s) 240, 286, 536, 1042
TaqI TCGA 4 cut(s) 14, 195, 478, 538
TasI AATT 9 cut(s) 127, 264, 491, 652, 663, 698, 880, 1080, 1282
TatI WGTACW 2 cut(s) 531, 1074
TfiI GAWTC 2 cut(s) 11, 1204
Tru1I TTAA 5 cut(s) 327, 435, 510, 1083, 1215
Tru9I TTAA 5 cut(s) 327, 435, 510, 1083, 1215
TscAI CASTG 5 cut(s) 208, 393, 605, 922, 963
TseFI GTSAC 1 cut(s) 369
TseI GCWGC 3 cut(s) 80, 1022, 1175
Tsp45I GTSAC 1 cut(s) 369
TspDTI ATGAA 6 cut(s) 377, 416, 743, 761, 822, 1268
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 5 cut(s) 208, 393, 605, 922, 963
VpaK11BI GGWCC 1 cut(s) 1118
XapI RAATTY 2 cut(s) 663, 880
XceI RCATGY 1 cut(s) 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 979
XspI CTAG 1 cut(s) 1262
Zsp2I ATGCAT 2 cut(s) 280, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.