FvH4_6g19650

Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014695
Physical Location & Seq
Reverse (-)
13135346 .. 13137329
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19650.t1

Sequence Viewer

Length: 1359 bp
ATGGCGAAAGAGTCGAATACTGTGATACTTGGTGCAAGTTCAATCATTATTTGCCTTCTTGCAACTGGTTTGCTTTATGCTGCATTGACCATAAACCCCTTTGAGACATGGAGGCACGAGCTATCAAATTCGGATGGTAAAAGCGTTGAAGGGAAAGAATCTCTGAGACTTCTCTTTAGAAGACTAGTAAGAGGAGAAGATCGATTTCAGCTCCATAGCAGCGGGTTATCTTGCCACTCTGATCTTCACTTTGAACAGTGTTTGGCTAGGAAACCAGTCATTATTGACAAGAATGCATCAACAGTATATATACCTTCCGATAATGAAGCCAATTCTGAATACAAGATTAAGCCATATGCAAGAAAAGAGGATGAGACTGCAATGAAGGTTGTCACACCTGTAAGAATAGTACATGGTAACATTACTCCACCAGCCTGTGATTTTATCCATAGAGTTCCAGCACTGATCTTCTCCTCAGGTGGTTTCACAGGAAACCTATTTCATGAGTTTAATGAGATCATCATCCCCTTGTTCCTCACCTGCCACCACTTTCGATCCCGCATCCAATTTGTCGTAACTGACTTTAAACCTTGGTGGGTGAAAAAGTACAGTCGAGTTCTATCCCATTTGTCCAGCCATGCTGTTATAAATCCGGTTGAGAATGGTAGTGTTCACTGCTTTCCTGGAGCTATCATGGGGCTGAGATATCATGACAACTTAGCTCTAAATTATACAGAAATTCCAGAAGGATACTCCATGCTTGATTTCAAACAATTCCTTAGAGAGTCATATATGCTGAAAATAAAGCATGTCTCGGAGATGAAAAGGCAAAGGCCAGGGCTACTACTCCTATCTCGTCGCGAAACAAGGAAGTTTCTGAATGAAGAGAAAATGATAGAAATGATGGAAGCATTAGGCTTTCAAGTCATTGCTGCAATGCCCAATCAGACGTCAAATTTGGACACATTTTCCGGCTTGGTGAACTCGTGCAGTATCATTGTTGGAGCTCATGGTGCTGGCCTTACAAATGCAGTGTTCTTGCCAACCAAGGCAGTGATAGTGCAAGTGGTACCTCTGGGGTTAGATTGGCCTTCAACTGCCTACTATGGTGAAACAGTAGGTGGAATGGGGTTGGAATACTTGGAGTACAAGATTAAAGCAGAGGAAAGTTCACTCATTGATATCTATGGTCCGGATCACCCAGTCATTACTGATCCCCAGTCTGTATTCGTGAAGGGCTATGAGGCTGCCAGGGCTGTTTATGTTGATGGCCAGAATCTGAAGATTAACTTGGTCAGGTTCAGGAAAACCCTTGTTGAAGCCATGAAACTTCTTGGACACTCTACTCCTGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 ko00515 ko01100 map00514 map00515 map01100
Pfam Domains
Protein Families
DUF563

Protein Analysis

453

Amino Acids

50.81

Weight (kDa)

7.68

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 266 - 354 1.5e-11 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23260 FvH4_6g19650
malus_domestica MD03G1195900.v1.1
prunus_persica Prupe.4G220000_v2.0.a1
pyrus_communis pycom03g14810
rosa_chinensis RchiOBHm_Chr5g0040951
rosa_laevigata RLG00000034017
rosa_multiflora Rmu_sc0006651.1_g000006 Rmu_sc0006651.1_g000009
rosa_roxburghii Rroxscaffold_1G00039740
rosa_rugosa Rorug05G0189000 Rorug05G0189100 Rorug05G0189200
rosa_samantha Rh5BG279500 Rh5CG311700 Rh5DG287600
rosa_wichuraiana Rw5G025800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 647
AarI CACCTGC 1 cut(s) 548
AatII GACGTC 1 cut(s) 953
Acc36I ACCTGC 1 cut(s) 548
Acc65I GGTACC 1 cut(s) 1069
AccB1I GGYRCC 1 cut(s) 1069
AccII CGCG 1 cut(s) 861
AccIII TCCGGA 1 cut(s) 1192
AciI CCGC 2 cut(s) 222, 559
AclWI GGATC 3 cut(s) 549, 1203, 1208
AcoI YGGCCR 1 cut(s) 1270
AcsI RAATTY 3 cut(s) 127, 738, 955
AcuI CTGAAG 1 cut(s) 1301
AcyI GRCGYC 1 cut(s) 950
AfaI GTAC 4 cut(s) 411, 608, 1071, 1148
AgsI TTSAA 7 cut(s) 42, 149, 254, 769, 923, 1095, 1319
AhlI ACTAGT 1 cut(s) 184
AjnI CCWGG 3 cut(s) 682, 835, 1250
AjuI GAANNNNNNNTTGG 2 cut(s) 1274, 1306
AluBI AGCT 5 cut(s) 121, 211, 689, 722, 1007
AluI AGCT 5 cut(s) 121, 211, 689, 722, 1007
Alw21I GWGCWC 1 cut(s) 1009
Alw26I GTCTC 4 cut(s) 98, 160, 368, 817
AlwI GGATC 3 cut(s) 549, 1203, 1208
AlwNI CAGNNNCTG 1 cut(s) 1279
Aor13HI TCCGGA 1 cut(s) 1192
AoxI GGCC 4 cut(s) 833, 1018, 1088, 1270
ApeKI GCWGC 4 cut(s) 80, 219, 932, 1247
ApoI RAATTY 3 cut(s) 127, 738, 955
ArsI GACNNNNNNTTYG 2 cut(s) 940, 972
Asp718I GGTACC 1 cut(s) 1069
AspS9I GGNCC 1 cut(s) 1190
AsuHPI GGTGA 5 cut(s) 529, 610, 991, 1121, 1190
AvaII GGWCC 1 cut(s) 1190
AxyI CCTNAGG 1 cut(s) 475
BalI TGGCCA 1 cut(s) 1272
BanI GGYRCC 1 cut(s) 1069
BanII GRGCYC 1 cut(s) 1009
BauI CACGAG 2 cut(s) 116, 985
BbsI GAAGAC 1 cut(s) 187
Bbv12I GWGCWC 1 cut(s) 1009
BbvI GCAGC 4 cut(s) 67, 231, 919, 1234
BccI CCATC 3 cut(s) 128, 898, 1262
BciT130I CCWGG 3 cut(s) 684, 837, 1252
BciVI GTATCC 1 cut(s) 743
BcoDI GTCTC 4 cut(s) 98, 160, 368, 817
BcuI ACTAGT 1 cut(s) 184
BfaI CTAG 2 cut(s) 185, 267
BfuAI ACCTGC 1 cut(s) 548
BfuI GTATCC 1 cut(s) 743
BisI GCNGC 4 cut(s) 81, 220, 933, 1248
BlsI GCNGC 4 cut(s) 82, 221, 934, 1249
Bme1390I CCNGG 3 cut(s) 684, 837, 1252
Bme18I GGWCC 1 cut(s) 1190
BmgT120I GGNCC 1 cut(s) 1190
BmiI GGNNCC 1 cut(s) 1071
BmrFI CCNGG 3 cut(s) 684, 837, 1252
BmrI ACTGGG 2 cut(s) 1196, 1213
BmsI GCATC 2 cut(s) 305, 570
BmuI ACTGGG 2 cut(s) 1196, 1213
BpiI GAAGAC 1 cut(s) 187
BpmI CTGGAG 1 cut(s) 705
Bsa29I ATCGAT 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 521
BsaHI GRCGYC 1 cut(s) 950
BsaJI CCNNGG 4 cut(s) 590, 836, 1047, 1251
BsaWI WCCGGW 2 cut(s) 652, 1192
Bse1I ACTGG 4 cut(s) 70, 275, 1202, 1219
Bse21I CCTNAGG 1 cut(s) 475
Bse3DI GCAATG 3 cut(s) 387, 927, 942
Bse8I GATNNNNATC 1 cut(s) 521
BseAI TCCGGA 1 cut(s) 1192
BseBI CCWGG 3 cut(s) 684, 837, 1252
BseCI ATCGAT 1 cut(s) 202
BseDI CCNNGG 4 cut(s) 590, 836, 1047, 1251
BseGI GGATG 4 cut(s) 139, 376, 522, 561
BseJI GATNNNNATC 1 cut(s) 521
BseMI GCAATG 3 cut(s) 387, 927, 942
BseMII CTCAG 3 cut(s) 155, 489, 692
BseNI ACTGG 4 cut(s) 70, 275, 1202, 1219
BseRI GAGGAG 2 cut(s) 207, 463
BseXI GCAGC 4 cut(s) 67, 231, 919, 1234
BsgI GTGCAG 1 cut(s) 1009
Bsh1236I CGCG 1 cut(s) 861
BshFI GGCC 4 cut(s) 835, 1020, 1090, 1272
BshNI GGYRCC 1 cut(s) 1069
BshVI ATCGAT 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 1009
BsiSI CCGG 3 cut(s) 653, 972, 1193
BsmAI GTCTC 4 cut(s) 98, 160, 368, 817
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 4 cut(s) 835, 1020, 1090, 1272
Bsp1286I GDGCHC 1 cut(s) 1009
Bsp13I TCCGGA 1 cut(s) 1192
Bsp143I GATC 7 cut(s) 199, 241, 465, 516, 554, 1195, 1213
Bsp68I TCGCGA 1 cut(s) 861
BspACI CCGC 2 cut(s) 222, 559
BspANI GGCC 4 cut(s) 835, 1020, 1090, 1272
BspCNI CTCAG 3 cut(s) 156, 488, 693
BspDI ATCGAT 1 cut(s) 202
BspEI TCCGGA 1 cut(s) 1192
BspFNI CGCG 1 cut(s) 861
BspHI TCATGA 2 cut(s) 502, 709
BspLI GGNNCC 1 cut(s) 1071
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 3 cut(s) 549, 1203, 1208
BspT107I GGYRCC 1 cut(s) 1069
BsrDI GCAATG 3 cut(s) 387, 927, 942
BsrI ACTGG 4 cut(s) 70, 275, 1202, 1219
BssECI CCNNGG 4 cut(s) 590, 836, 1047, 1251
BssMI GATC 7 cut(s) 199, 241, 465, 516, 554, 1195, 1213
BssNI GRCGYC 1 cut(s) 950
BssSI CACGAG 2 cut(s) 116, 985
BssT1I CCWWGG 2 cut(s) 590, 1047
Bst2BI CACGAG 2 cut(s) 116, 985
Bst2UI CCWGG 3 cut(s) 684, 837, 1252
Bst4CI ACNGT 5 cut(s) 22, 258, 304, 611, 1117
Bst6I CTCTTC 1 cut(s) 879
BstACI GRCGYC 1 cut(s) 950
BstC8I GCNNGC 1 cut(s) 1018
BstDEI CTNAG 5 cut(s) 164, 475, 701, 718, 779
BstF5I GGATG 4 cut(s) 139, 376, 522, 561
BstFNI CGCG 1 cut(s) 861
BstKTI GATC 7 cut(s) 202, 244, 468, 519, 557, 1198, 1216
BstMAI GTCTC 4 cut(s) 98, 160, 368, 817
BstMBI GATC 7 cut(s) 199, 241, 465, 516, 554, 1195, 1213
BstMWI GCNNNNNNNGC 2 cut(s) 1013, 1253
BstNI CCWGG 3 cut(s) 684, 837, 1252
BstNSI RCATGY 1 cut(s) 812
BstSCI CCNGG 3 cut(s) 682, 835, 1250
BstUI CGCG 1 cut(s) 861
BstV1I GCAGC 4 cut(s) 67, 231, 919, 1234
BstV2I GAAGAC 1 cut(s) 187
Bsu15I ATCGAT 1 cut(s) 202
Bsu36I CCTNAGG 1 cut(s) 475
BsuI GTATCC 1 cut(s) 743
BsuRI GGCC 4 cut(s) 835, 1020, 1090, 1272
BsuTUI ATCGAT 1 cut(s) 202
BtsCI GGATG 4 cut(s) 139, 376, 522, 561
BtsI GCAGTG 3 cut(s) 673, 1038, 1059
BtsIMutI CAGTG 5 cut(s) 263, 461, 673, 1038, 1059
BtuMI TCGCGA 1 cut(s) 861
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 1 cut(s) 1018
CaiI CAGNNNCTG 1 cut(s) 1279
CciI TCATGA 2 cut(s) 502, 709
Cfr13I GGNCC 1 cut(s) 1190
ClaI ATCGAT 1 cut(s) 202
Csp6I GTAC 4 cut(s) 410, 607, 1070, 1147
CviQI GTAC 4 cut(s) 410, 607, 1070, 1147
DdeI CTNAG 5 cut(s) 164, 475, 701, 718, 779
DpnI GATC 7 cut(s) 201, 243, 467, 518, 556, 1197, 1215
DpnII GATC 7 cut(s) 199, 241, 465, 516, 554, 1195, 1213
DraI TTTAAA 1 cut(s) 586
EaeI YGGCCR 1 cut(s) 1270
Eam1104I CTCTTC 1 cut(s) 879
EarI CTCTTC 1 cut(s) 879
Ecl136II GAGCTC 1 cut(s) 1007
Eco130I CCWWGG 2 cut(s) 590, 1047
Eco24I GRGCYC 1 cut(s) 1009
Eco32I GATATC 2 cut(s) 707, 1183
Eco47I GGWCC 1 cut(s) 1190
Eco53kI GAGCTC 1 cut(s) 1007
Eco57I CTGAAG 1 cut(s) 1301
Eco81I CCTNAGG 1 cut(s) 475
EcoICRI GAGCTC 1 cut(s) 1007
EcoRII CCWGG 3 cut(s) 682, 835, 1250
EcoRV GATATC 2 cut(s) 707, 1183
EcoT14I CCWWGG 2 cut(s) 590, 1047
EcoT22I ATGCAT 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 1009
ErhI CCWWGG 2 cut(s) 590, 1047
FalI AAGNNNNNCTT 2 cut(s) 1274, 1306
FauI CCCGC 2 cut(s) 215, 566
FauNDI CATATG 1 cut(s) 355
Fnu4HI GCNGC 4 cut(s) 81, 220, 933, 1248
FokI GGATG 4 cut(s) 146, 383, 509, 548
FriOI GRGCYC 1 cut(s) 1009
Fsp4HI GCNGC 4 cut(s) 81, 220, 933, 1248
FspBI CTAG 2 cut(s) 185, 267
GluI GCNGC 4 cut(s) 81, 220, 933, 1248
GsuI CTGGAG 1 cut(s) 705
HaeIII GGCC 4 cut(s) 835, 1020, 1090, 1272
HapII CCGG 3 cut(s) 653, 972, 1193
Hin1I GRCGYC 1 cut(s) 950
HinfI GANTC 4 cut(s) 11, 158, 785, 1276
HpaII CCGG 3 cut(s) 653, 972, 1193
HphI GGTGA 5 cut(s) 529, 610, 991, 1121, 1190
Hpy166II GTNNAC 3 cut(s) 673, 982, 1172
Hpy188I TCNGA 9 cut(s) 133, 165, 241, 319, 337, 817, 879, 948, 1281
Hpy188III TCNNGA 8 cut(s) 503, 710, 743, 860, 1193, 1231, 1303, 1349
Hpy8I GTNNAC 3 cut(s) 673, 982, 1172
Hpy99I CGWCG 1 cut(s) 861
HpyAV CCTTC 7 cut(s) 65, 143, 324, 379, 740, 1101, 1228
HpyCH4III ACNGT 5 cut(s) 22, 258, 304, 611, 1117
HpyCH4IV ACGT 1 cut(s) 950
HpyF10VI GCNNNNNNNGC 2 cut(s) 1013, 1253
HpyF3I CTNAG 5 cut(s) 164, 475, 701, 718, 779
HpySE526I ACGT 1 cut(s) 950
Hsp92I GRCGYC 1 cut(s) 950
Kpn2I TCCGGA 1 cut(s) 1192
KpnI GGTACC 1 cut(s) 1073
Kzo9I GATC 7 cut(s) 199, 241, 465, 516, 554, 1195, 1213
LmnI GCTCC 3 cut(s) 216, 686, 1004
Lsp1109I GCAGC 4 cut(s) 67, 231, 919, 1234
LweI GCATC 2 cut(s) 305, 570
MaeI CTAG 2 cut(s) 185, 267
MaeII ACGT 1 cut(s) 950
MaeIII GTNAC 3 cut(s) 391, 416, 574
MalI GATC 7 cut(s) 201, 243, 467, 518, 556, 1197, 1215
MboI GATC 7 cut(s) 199, 241, 465, 516, 554, 1195, 1213
MboII GAAGA 6 cut(s) 192, 209, 236, 460, 896, 1294
MhlI GDGCHC 1 cut(s) 1009
MlsI TGGCCA 1 cut(s) 1272
MluCI AATT 8 cut(s) 127, 331, 566, 727, 738, 773, 955, 1354
MluNI TGGCCA 1 cut(s) 1272
MlyI GAGTC 2 cut(s) 20, 794
MmeI TCCRAC 2 cut(s) 982, 1113
MnlI CCTC 8 cut(s) 105, 185, 361, 484, 545, 1083, 1156, 1237
Mox20I TGGCCA 1 cut(s) 1272
Mph1103I ATGCAT 1 cut(s) 298
MroI TCCGGA 1 cut(s) 1192
MscI TGGCCA 1 cut(s) 1272
MseI TTAA 6 cut(s) 348, 510, 585, 1155, 1287, 1357
Msp20I TGGCCA 1 cut(s) 1272
MspA1I CMGCKG 1 cut(s) 222
MspI CCGG 3 cut(s) 653, 972, 1193
MspR9I CCNGG 3 cut(s) 684, 837, 1252
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 3 cut(s) 684, 837, 1252
MvnI CGCG 1 cut(s) 861
MwoI GCNNNNNNNGC 2 cut(s) 1013, 1253
NdeI CATATG 1 cut(s) 355
NdeII GATC 7 cut(s) 199, 241, 465, 516, 554, 1195, 1213
NlaIV GGNNCC 1 cut(s) 1071
NmuCI GTSAC 1 cut(s) 391
NruI TCGCGA 1 cut(s) 861
NsiI ATGCAT 1 cut(s) 298
NspI RCATGY 1 cut(s) 812
PagI TCATGA 2 cut(s) 502, 709
PaqCI CACCTGC 1 cut(s) 548
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 158, 1276
PfoI TCCNGGA 1 cut(s) 682
PkrI GCNGC 4 cut(s) 82, 221, 934, 1249
PleI GAGTC 2 cut(s) 19, 793
PpsI GAGTC 2 cut(s) 19, 793
PsiI TTATAA 1 cut(s) 647
Psp124BI GAGCTC 1 cut(s) 1009
Psp6I CCWGG 3 cut(s) 682, 835, 1250
PspGI CCWGG 3 cut(s) 682, 835, 1250
PspN4I GGNNCC 1 cut(s) 1071
PspPI GGNCC 1 cut(s) 1190
PstNI CAGNNNCTG 1 cut(s) 1279
RruI TCGCGA 1 cut(s) 861
RsaI GTAC 4 cut(s) 411, 608, 1071, 1148
RsaNI GTAC 4 cut(s) 410, 607, 1070, 1147
SacI GAGCTC 1 cut(s) 1009
SaqAI TTAA 6 cut(s) 348, 510, 585, 1155, 1287, 1357
SatI GCNGC 4 cut(s) 81, 220, 933, 1248
Sau3AI GATC 7 cut(s) 199, 241, 465, 516, 554, 1195, 1213
Sau96I GGNCC 1 cut(s) 1190
SchI GAGTC 2 cut(s) 20, 794
ScrFI CCNGG 3 cut(s) 684, 837, 1252
SduI GDGCHC 1 cut(s) 1009
SfaNI GCATC 2 cut(s) 305, 570
SinI GGWCC 1 cut(s) 1190
SpeI ACTAGT 1 cut(s) 184
Sse9I AATT 8 cut(s) 127, 331, 566, 727, 738, 773, 955, 1354
SsiI CCGC 2 cut(s) 222, 559
SspMI CTAG 2 cut(s) 185, 267
SstI GAGCTC 1 cut(s) 1009
StyD4I CCNGG 3 cut(s) 682, 835, 1250
StyI CCWWGG 2 cut(s) 590, 1047
TaaI ACNGT 5 cut(s) 22, 258, 304, 611, 1117
TaiI ACGT 1 cut(s) 953
TaqI TCGA 4 cut(s) 14, 202, 553, 613
TasI AATT 8 cut(s) 127, 331, 566, 727, 738, 773, 955, 1354
TatI WGTACW 3 cut(s) 409, 606, 1146
TfiI GAWTC 2 cut(s) 158, 1276
Tru1I TTAA 6 cut(s) 348, 510, 585, 1155, 1287, 1357
Tru9I TTAA 6 cut(s) 348, 510, 585, 1155, 1287, 1357
TscAI CASTG 5 cut(s) 263, 468, 680, 1038, 1059
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 4 cut(s) 80, 219, 932, 1247
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 6 cut(s) 339, 398, 491, 836, 897, 1340
TspRI CASTG 5 cut(s) 263, 468, 680, 1038, 1059
VpaK11BI GGWCC 1 cut(s) 1190
XapI RAATTY 3 cut(s) 127, 738, 955
XceI RCATGY 1 cut(s) 812
XspI CTAG 2 cut(s) 185, 267
ZraI GACGTC 1 cut(s) 951
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.