MD03G1195900.v1.1

Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014695
Physical Location & Seq
Forward (+)
26853643 .. 26855299
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1195900.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGATGGCGAAGGACTCAAAAACTGGGGTGGTTGGTACAACAATTTCAATGATTTTCTTGCTTACAATTGGTTTGCTTTATGCTGCATTCACCCCCAATATTAATCCCTTTGAGACATGGATGCACACCAGAAAAGATGACGAAGTCTTTGGAGTGGAAGAGCCTCTCAATTCTTTCTTCAAAAGACTAGTTAAAGGGGTAGATAGAATTCAACTCGAAACCACAGGGTTGTCTTGCCACTCTGACGTTCACTCGGAAGTGTGTGTGGCTAATAGACCAGTTATAGTTGACAAGAGTGCTTTAACAGTTTATGTACCTTCAAATGAAGCCCAAGTTGAATACAAGGTTCAGCCATATGCAAGGAAAGAAGATGGGACTGCAATGAAGGCTGTCACACCAGTGCAAATACTACATGGAAACATTAGTAATCCTCCGGCTTGCAATTTTTCTTACAACATCCCAGCTATGATATTCTCTTCTGGTGGCTTCACCGGAAACCTATTTCACGAATTCAATGAGATCATCATCCCCTTGTTCATCATGTCACGCAGCTTTCGATCCCAGCTTCAGTTTGTCATAACTGATTTTAAGCCATGGTGGGTTAGCAAGTACGAACGAATTCTATCATCGTTGTCCAGTTATGATGTGATAAATTCAGTTGAGAAAGAAGGTGTTCACTGCTATCCTGGAGCTGTCATAGGCCTTAAGTTTCATGACAACTTAGCGCTAAATTCTTCCGACATTCCAGGAGGGTACTCCATGTTTGACTTCAAGCAGTTCCTAAGAGAATCATACATGCTGAAAATGAAGGATGTCTCAAATCAGATGGGGAAAAAACCAGTGCTCGTTCTTATATCTCGTCGCAAAACAAGGAAGTTTTTGAATGAAGATAAGATGGTGGAAATGATGGAAGAATTAGGCTTTCAGGTTATCGCTGCAACGCCTAACCAGACATCTAATTTGGACACATTTTCGGGCTTAGTGAACTCGTGCAGTGTCATGGTTGGAGCTCACGGTGCTGGCCTTACAAATGCACTGTTCTTGCCAGCTGGGGCAGTGTTAGTGCAAGTGGTACCGCTAGGCCTAGATTGGCCGTCCGCTAACTATTATGGTGGACCGGCGAGTGAAATGGGGCTGAATTACTTGGAATACAAGATTAAACCAGAGGAGAGTTCACTGATTGATGACTATGGTCCAGATCACCCTGTCATCACGGATCCCATGTCTATTTCCTTGAAGGGCTATCGGGCTTCCAGGGCTGTGTACGTTGATGGACAGAATCTGAAGGTTAACTTGGTGAGGTTCCGAGAAACACTCGTTGAAGCTATGAAACTTGTTGGAGGCTCCACTCCTTCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 ko00515 ko01100 map00514 map00515 map01100
Pfam Domains
Protein Families
DUF563

Protein Analysis

454

Amino Acids

50.3

Weight (kDa)

6.17

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 242 - 355 3.8e-12 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23260 FvH4_6g19650
malus_domestica MD03G1195900.v1.1
prunus_persica Prupe.4G220000_v2.0.a1
pyrus_communis pycom03g14810
rosa_chinensis RchiOBHm_Chr5g0040951
rosa_laevigata RLG00000034017
rosa_multiflora Rmu_sc0006651.1_g000006 Rmu_sc0006651.1_g000009
rosa_roxburghii Rroxscaffold_1G00039740
rosa_rugosa Rorug05G0189000 Rorug05G0189100 Rorug05G0189200
rosa_samantha Rh5BG279500 Rh5CG311700 Rh5DG287600
rosa_wichuraiana Rw5G025800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1072
AccB1I GGYRCC 1 cut(s) 1072
AciI CCGC 2 cut(s) 1076, 1098
AclWI GGATC 3 cut(s) 552, 1211, 1224
AcoI YGGCCR 1 cut(s) 1091
AcsI RAATTY 5 cut(s) 207, 509, 618, 652, 730
AcuI CTGAAG 2 cut(s) 551, 1304
AfaI GTAC 6 cut(s) 37, 315, 611, 755, 1074, 1265
AfeI AGCGCT 1 cut(s) 726
AflII CTTAAG 1 cut(s) 704
AhlI ACTAGT 1 cut(s) 187
AjnI CCWGG 3 cut(s) 685, 745, 1253
AjuI GAANNNNNNNTTGG 2 cut(s) 1277, 1309
AleI CACNNNNGTG 1 cut(s) 398
AluBI AGCT 7 cut(s) 464, 552, 565, 692, 1010, 1049, 1325
AluI AGCT 7 cut(s) 464, 552, 565, 692, 1010, 1049, 1325
Alw21I GWGCWC 2 cut(s) 846, 1012
Alw26I GTCTC 2 cut(s) 107, 820
AlwI GGATC 3 cut(s) 552, 1211, 1224
AlwNI CAGNNNCTG 1 cut(s) 1282
Aor51HI AGCGCT 1 cut(s) 726
AoxI GGCC 4 cut(s) 700, 1021, 1081, 1091
ApeKI GCWGC 3 cut(s) 83, 549, 935
ApoI RAATTY 5 cut(s) 207, 509, 618, 652, 730
ArsI GACNNNNNNTTYG 4 cut(s) 131, 163, 943, 975
AseI ATTAAT 1 cut(s) 102
Asp700I GAANNNNTTC 2 cut(s) 618, 672
Asp718I GGTACC 1 cut(s) 1072
AspLEI GCGC 1 cut(s) 727
AspS9I GGNCC 2 cut(s) 1115, 1193
AsuHPI GGTGA 4 cut(s) 82, 481, 1193, 1309
AvaII GGWCC 2 cut(s) 1115, 1193
BamHI GGATCC 1 cut(s) 1216
BanI GGYRCC 1 cut(s) 1072
BanII GRGCYC 1 cut(s) 1012
BauI CACGAG 1 cut(s) 988
Bbv12I GWGCWC 2 cut(s) 846, 1012
BbvI GCAGC 3 cut(s) 70, 561, 922
BccI CCATC 5 cut(s) 365, 820, 889, 901, 1265
BceAI ACGGC 1 cut(s) 1078
BciT130I CCWGG 3 cut(s) 687, 747, 1255
BcoDI GTCTC 2 cut(s) 107, 820
BcuI ACTAGT 1 cut(s) 187
BfaI CTAG 3 cut(s) 188, 1079, 1085
BfoI RGCGCY 1 cut(s) 728
BfrI CTTAAG 1 cut(s) 704
BisI GCNGC 3 cut(s) 84, 550, 936
BlsI GCNGC 3 cut(s) 85, 551, 937
Bme1390I CCNGG 3 cut(s) 687, 747, 1255
Bme18I GGWCC 2 cut(s) 1115, 1193
BmgT120I GGNCC 2 cut(s) 1115, 1193
BmiI GGNNCC 4 cut(s) 1074, 1218, 1304, 1345
BmrFI CCNGG 3 cut(s) 687, 747, 1255
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 1 cut(s) 111
BmuI ACTGGG 1 cut(s) 33
BoxI GACNNNNGTC 1 cut(s) 1191
BplI GAGNNNNNCTC 2 cut(s) 1299, 1331
BpmI CTGGAG 1 cut(s) 708
BsaBI GATNNNNATC 1 cut(s) 524
BsaJI CCNNGG 2 cut(s) 593, 1254
BsaWI WCCGGW 1 cut(s) 491
Bse118I RCCGGY 1 cut(s) 1117
Bse1I ACTGG 5 cut(s) 28, 278, 398, 636, 839
Bse3DI GCAATG 1 cut(s) 387
Bse8I GATNNNNATC 1 cut(s) 524
BseBI CCWGG 3 cut(s) 687, 747, 1255
BseDI CCNNGG 2 cut(s) 593, 1254
BseGI GGATG 4 cut(s) 126, 456, 525, 817
BseJI GATNNNNATC 1 cut(s) 524
BseMI GCAATG 1 cut(s) 387
BseNI ACTGG 5 cut(s) 28, 278, 398, 636, 839
BseRI GAGGAG 1 cut(s) 1181
BseXI GCAGC 3 cut(s) 70, 561, 922
BseYI CCCAGC 3 cut(s) 460, 561, 1049
BsgI GTGCAG 1 cut(s) 1012
BshFI GGCC 4 cut(s) 702, 1023, 1083, 1093
BshNI GGYRCC 1 cut(s) 1072
BsiHKAI GWGCWC 2 cut(s) 846, 1012
BsiSI CCGG 3 cut(s) 434, 492, 1118
BslFI GGGAC 1 cut(s) 388
BsmAI GTCTC 2 cut(s) 107, 820
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 86
BsnI GGCC 4 cut(s) 702, 1023, 1083, 1093
Bsp1286I GDGCHC 2 cut(s) 846, 1012
Bsp143I GATC 4 cut(s) 519, 557, 1198, 1216
Bsp19I CCATGG 1 cut(s) 593
BspACI CCGC 2 cut(s) 1076, 1098
BspANI GGCC 4 cut(s) 702, 1023, 1083, 1093
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 4 cut(s) 1074, 1218, 1304, 1345
BspPI GGATC 3 cut(s) 552, 1211, 1224
BspQI GCTCTTC 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 1072
BspTI CTTAAG 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 387
BsrFI RCCGGY 1 cut(s) 1117
BsrI ACTGG 5 cut(s) 28, 278, 398, 636, 839
BssAI RCCGGY 1 cut(s) 1117
BssECI CCNNGG 2 cut(s) 593, 1254
BssMI GATC 4 cut(s) 519, 557, 1198, 1216
BssSI CACGAG 1 cut(s) 988
BssT1I CCWWGG 1 cut(s) 593
Bst2BI CACGAG 1 cut(s) 988
Bst2UI CCWGG 3 cut(s) 687, 747, 1255
Bst4CI ACNGT 3 cut(s) 307, 1016, 1038
Bst6I CTCTTC 2 cut(s) 153, 481
BstAFI CTTAAG 1 cut(s) 704
BstC8I GCNNGC 3 cut(s) 439, 1021, 1047
BstDEI CTNAG 3 cut(s) 721, 782, 979
BstDSI CCRYGG 1 cut(s) 593
BstF5I GGATG 4 cut(s) 126, 456, 525, 817
BstH2I RGCGCY 1 cut(s) 728
BstHHI GCGC 1 cut(s) 727
BstKTI GATC 4 cut(s) 522, 560, 1201, 1219
BstMAI GTCTC 2 cut(s) 107, 820
BstMBI GATC 4 cut(s) 519, 557, 1198, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 386, 1016, 1256
BstNI CCWGG 3 cut(s) 687, 747, 1255
BstNSI RCATGY 1 cut(s) 799
BstPAI GACNNNNGTC 1 cut(s) 1191
BstSCI CCNGG 3 cut(s) 685, 745, 1253
BstV1I GCAGC 3 cut(s) 70, 561, 922
BstX2I RGATCY 1 cut(s) 1216
BstYI RGATCY 1 cut(s) 1216
BsuRI GGCC 4 cut(s) 702, 1023, 1083, 1093
BtgI CCRYGG 1 cut(s) 593
BtsCI GGATG 4 cut(s) 126, 456, 525, 817
BtsI GCAGTG 3 cut(s) 676, 1000, 1062
BtsIMutI CAGTG 7 cut(s) 405, 676, 846, 1000, 1034, 1062, 1175
Cac8I GCNNGC 3 cut(s) 439, 1021, 1047
CaiI CAGNNNCTG 1 cut(s) 1282
CciI TCATGA 1 cut(s) 712
CfoI GCGC 1 cut(s) 727
Cfr10I RCCGGY 1 cut(s) 1117
Cfr13I GGNCC 2 cut(s) 1115, 1193
Csp6I GTAC 6 cut(s) 36, 314, 610, 754, 1073, 1264
CviAII CATG 9 cut(s) 117, 413, 541, 594, 713, 760, 796, 1000, 1222
CviQI GTAC 6 cut(s) 36, 314, 610, 754, 1073, 1264
DdeI CTNAG 3 cut(s) 721, 782, 979
DpnI GATC 4 cut(s) 521, 559, 1200, 1218
DpnII GATC 4 cut(s) 519, 557, 1198, 1216
EaeI YGGCCR 1 cut(s) 1091
Eam1104I CTCTTC 2 cut(s) 153, 481
EarI CTCTTC 2 cut(s) 153, 481
Ecl136II GAGCTC 1 cut(s) 1010
Eco130I CCWWGG 1 cut(s) 593
Eco147I AGGCCT 2 cut(s) 702, 1083
Eco24I GRGCYC 1 cut(s) 1012
Eco47I GGWCC 2 cut(s) 1115, 1193
Eco47III AGCGCT 1 cut(s) 726
Eco53kI GAGCTC 1 cut(s) 1010
Eco57I CTGAAG 2 cut(s) 551, 1304
EcoICRI GAGCTC 1 cut(s) 1010
EcoRI GAATTC 3 cut(s) 207, 509, 618
EcoRII CCWGG 3 cut(s) 685, 745, 1253
EcoT14I CCWWGG 1 cut(s) 593
EcoT38I GRGCYC 1 cut(s) 1012
ErhI CCWWGG 1 cut(s) 593
FaeI CATG 9 cut(s) 120, 416, 544, 597, 716, 763, 799, 1003, 1225
FalI AAGNNNNNCTT 2 cut(s) 1277, 1309
FaqI GGGAC 1 cut(s) 388
FatI CATG 9 cut(s) 116, 412, 540, 593, 712, 759, 795, 999, 1221
FauNDI CATATG 1 cut(s) 355
Fnu4HI GCNGC 3 cut(s) 84, 550, 936
FokI GGATG 4 cut(s) 133, 443, 512, 824
FriOI GRGCYC 1 cut(s) 1012
Fsp4HI GCNGC 3 cut(s) 84, 550, 936
FspBI CTAG 3 cut(s) 188, 1079, 1085
GlaI GCGC 1 cut(s) 726
GluI GCNGC 3 cut(s) 84, 550, 936
GsaI CCCAGC 3 cut(s) 464, 565, 1053
GsuI CTGGAG 1 cut(s) 708
HaeII RGCGCY 1 cut(s) 728
HaeIII GGCC 4 cut(s) 702, 1023, 1083, 1093
HapII CCGG 3 cut(s) 434, 492, 1118
HhaI GCGC 1 cut(s) 727
Hin1II CATG 9 cut(s) 120, 416, 544, 597, 716, 763, 799, 1003, 1225
Hin6I GCGC 1 cut(s) 725
HinP1I GCGC 1 cut(s) 725
HincII GTYRAC 2 cut(s) 289, 1291
HindII GTYRAC 2 cut(s) 289, 1291
HinfI GANTC 3 cut(s) 14, 788, 1279
HpaI GTTAAC 1 cut(s) 1291
HpaII CCGG 3 cut(s) 434, 492, 1118
HphI GGTGA 4 cut(s) 82, 481, 1193, 1309
Hpy166II GTNNAC 8 cut(s) 250, 289, 676, 985, 1115, 1175, 1264, 1291
Hpy188I TCNGA 6 cut(s) 244, 256, 739, 825, 1284, 1307
Hpy188III TCNNGA 3 cut(s) 506, 713, 1196
Hpy8I GTNNAC 8 cut(s) 250, 289, 676, 985, 1115, 1175, 1264, 1291
Hpy99I CGWCG 1 cut(s) 864
HpyAV CCTTC 8 cut(s) 4, 327, 379, 662, 802, 1231, 1279, 1362
HpyCH4III ACNGT 3 cut(s) 307, 1016, 1038
HpyCH4IV ACGT 2 cut(s) 246, 1266
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 1016, 1256
HpyF3I CTNAG 3 cut(s) 721, 782, 979
HpySE526I ACGT 2 cut(s) 246, 1266
Hsp92II CATG 9 cut(s) 120, 416, 544, 597, 716, 763, 799, 1003, 1225
HspAI GCGC 1 cut(s) 725
KpnI GGTACC 1 cut(s) 1076
KspAI GTTAAC 1 cut(s) 1291
Kzo9I GATC 4 cut(s) 519, 557, 1198, 1216
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 3 cut(s) 689, 1007, 1349
Lsp1109I GCAGC 3 cut(s) 70, 561, 922
LweI GCATC 1 cut(s) 111
MaeI CTAG 3 cut(s) 188, 1079, 1085
MaeII ACGT 2 cut(s) 246, 1266
MaeIII GTNAC 2 cut(s) 391, 543
MalI GATC 4 cut(s) 521, 559, 1200, 1218
MboI GATC 4 cut(s) 519, 557, 1198, 1216
MboII GAAGA 7 cut(s) 169, 170, 380, 468, 726, 899, 923
MfeI CAATTG 1 cut(s) 66
MflI RGATCY 1 cut(s) 1216
MhlI GDGCHC 2 cut(s) 846, 1012
MlyI GAGTC 1 cut(s) 8
MmeI TCCRAC 3 cut(s) 762, 985, 1318
MnlI CCTC 6 cut(s) 174, 441, 743, 1159, 1293, 1334
MroXI GAANNNNTTC 2 cut(s) 618, 672
MseI TTAA 8 cut(s) 102, 192, 302, 588, 705, 1158, 1290, 1360
MslI CAYNNNNRTG 1 cut(s) 398
MspA1I CMGCKG 1 cut(s) 1049
MspCI CTTAAG 1 cut(s) 704
MspI CCGG 3 cut(s) 434, 492, 1118
MspR9I CCNGG 3 cut(s) 687, 747, 1255
MunI CAATTG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 86
MvaI CCWGG 3 cut(s) 687, 747, 1255
MwoI GCNNNNNNNGC 3 cut(s) 386, 1016, 1256
NcoI CCATGG 1 cut(s) 593
NdeI CATATG 1 cut(s) 355
NdeII GATC 4 cut(s) 519, 557, 1198, 1216
NlaIII CATG 9 cut(s) 120, 416, 544, 597, 716, 763, 799, 1003, 1225
NlaIV GGNNCC 4 cut(s) 1074, 1218, 1304, 1345
NmuCI GTSAC 2 cut(s) 391, 543
NspI RCATGY 1 cut(s) 799
OliI CACNNNNGTG 1 cut(s) 398
PagI TCATGA 1 cut(s) 712
PceI AGGCCT 2 cut(s) 702, 1083
PciSI GCTCTTC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 553
PctI GAATGC 1 cut(s) 86
PdmI GAANNNNTTC 2 cut(s) 618, 672
PfeI GAWTC 2 cut(s) 788, 1279
PflFI GACNNNGTC 1 cut(s) 143
PfoI TCCNGGA 2 cut(s) 685, 745
PkrI GCNGC 3 cut(s) 85, 551, 937
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
PshAI GACNNNNGTC 1 cut(s) 1191
PshBI ATTAAT 1 cut(s) 102
Psp124BI GAGCTC 1 cut(s) 1012
Psp6I CCWGG 3 cut(s) 685, 745, 1253
PspFI CCCAGC 3 cut(s) 460, 561, 1049
PspGI CCWGG 3 cut(s) 685, 745, 1253
PspN4I GGNNCC 4 cut(s) 1074, 1218, 1304, 1345
PspPI GGNCC 2 cut(s) 1115, 1193
PstNI CAGNNNCTG 1 cut(s) 1282
PsuI RGATCY 1 cut(s) 1216
PsyI GACNNNGTC 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 1049
RsaI GTAC 6 cut(s) 37, 315, 611, 755, 1074, 1265
RsaNI GTAC 6 cut(s) 36, 314, 610, 754, 1073, 1264
RseI CAYNNNNRTG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 1012
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 8 cut(s) 102, 192, 302, 588, 705, 1158, 1290, 1360
SatI GCNGC 3 cut(s) 84, 550, 936
Sau3AI GATC 4 cut(s) 519, 557, 1198, 1216
Sau96I GGNCC 2 cut(s) 1115, 1193
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 3 cut(s) 687, 747, 1255
SduI GDGCHC 2 cut(s) 846, 1012
SfaNI GCATC 1 cut(s) 111
SinI GGWCC 2 cut(s) 1115, 1193
SmiMI CAYNNNNRTG 1 cut(s) 398
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
SpeI ACTAGT 1 cut(s) 187
SseBI AGGCCT 2 cut(s) 702, 1083
SsiI CCGC 2 cut(s) 1076, 1098
SspI AATATT 1 cut(s) 100
SspMI CTAG 3 cut(s) 188, 1079, 1085
SstI GAGCTC 1 cut(s) 1012
StuI AGGCCT 2 cut(s) 702, 1083
StyD4I CCNGG 3 cut(s) 685, 745, 1253
StyI CCWWGG 1 cut(s) 593
TaaI ACNGT 3 cut(s) 307, 1016, 1038
TaiI ACGT 2 cut(s) 249, 1269
TaqI TCGA 2 cut(s) 216, 556
TfiI GAWTC 2 cut(s) 788, 1279
Tru1I TTAA 8 cut(s) 102, 192, 302, 588, 705, 1158, 1290, 1360
Tru9I TTAA 8 cut(s) 102, 192, 302, 588, 705, 1158, 1290, 1360
TscAI CASTG 7 cut(s) 405, 683, 846, 1000, 1041, 1062, 1182
TseFI GTSAC 2 cut(s) 391, 543
TseI GCWGC 3 cut(s) 83, 549, 935
Tsp45I GTSAC 2 cut(s) 391, 543
TspDTI ATGAA 7 cut(s) 339, 398, 526, 701, 821, 900, 1343
TspGWI ACGGA 1 cut(s) 1229
TspRI CASTG 7 cut(s) 405, 683, 846, 1000, 1041, 1062, 1182
Tth111I GACNNNGTC 1 cut(s) 143
Vha464I CTTAAG 1 cut(s) 704
VpaK11BI GGWCC 2 cut(s) 1115, 1193
VspI ATTAAT 1 cut(s) 102
XapI RAATTY 5 cut(s) 207, 509, 618, 652, 730
XceI RCATGY 1 cut(s) 799
XmnI GAANNNNTTC 2 cut(s) 618, 672
XspI CTAG 3 cut(s) 188, 1079, 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.