FvH4_3g25170

Domain of unknown function (DUF4602)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007194
Physical Location & Seq
Forward (+)
18155496 .. 18162069
6574 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25170.t2

Sequence Viewer

Length: 594 bp
ATGCACAAGAACAAGGGAGGAGACGAAAGAGGGCGATCCTCTACGAGACCAATGGGTAGAAACAACCAAAATCCACACACCAAGGACCAAGAATTGGACTCGAACGACCTCAGAACGGACATGAGGGAGATTTACAAGCGTATTGAGCTCCTGGGCTCTTCTCATATGACATGGAAGGAGAGGAAGGAGTTGGAGAACAAGAAGGTGGTTGCTCTAGGTGGAAAGCCTCCAAAGAAGCAGAGACTACCTCTAAGTATAGCAAGAGTGGTCATGAAGAAACAAAAGGACAGGGAGCAAAAGCAGCTGCAAGAGAACGTGATTCTTGGACGTTTTGGAGGAAAATCCAGAGGTGGGTCGAAAAGATCTGTGGAGAAGCGAAGGCCTGAGGATAGGGTTCTAAGAGCAAGTGAAGGTTACTTCAAAAATGGTGTCCTTGATGTGAAACATTTGTTACGTCCGACATCATCCAGAGACAGGGATGGTGGTGCCCATGTGAACTTCACAGGTCCAAAGAAGGGAGGTGGAAAGAAGGGTGGTGGAAAGAAGGGAGGTGGAAAGAACAAAGGTAAGAAGGGTGGTAAGAAACGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4602

Protein Analysis

198

Amino Acids

22.12

Weight (kDa)

10.83

Isoelectric Point (pI)

54.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSAF1 PF15375 13 - 127 7.6e-16 40S small subunit processome assembly factor 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 485
AccB7I CCANNNNNTGG 1 cut(s) 94
AclWI GGATC 1 cut(s) 30
AfiI CCNNNNNNNGG 5 cut(s) 94, 115, 351, 474, 515
AgsI TTSAA 1 cut(s) 421
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 2 cut(s) 148, 304
AluI AGCT 2 cut(s) 148, 304
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 4 cut(s) 15, 40, 235, 465
AlwI GGATC 1 cut(s) 30
AoxI GGCC 1 cut(s) 380
ApeKI GCWGC 2 cut(s) 301, 304
ArsI GACNNNNNNTTYG 2 cut(s) 414, 446
AspS9I GGNCC 2 cut(s) 85, 506
AvaII GGWCC 2 cut(s) 85, 506
AxyI CCTNAGG 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 485
BanII GRGCYC 2 cut(s) 150, 158
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 2 cut(s) 291, 313
BccI CCATC 1 cut(s) 473
BciT130I CCWGG 1 cut(s) 152
BcoDI GTCTC 4 cut(s) 15, 40, 235, 465
BfaI CTAG 1 cut(s) 215
BglII AGATCT 1 cut(s) 362
BisI GCNGC 2 cut(s) 302, 305
BlsI GCNGC 2 cut(s) 303, 306
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 2 cut(s) 85, 506
BmgT120I GGNCC 2 cut(s) 85, 506
BmiI GGNNCC 1 cut(s) 487
BmrFI CCNGG 1 cut(s) 152
BplI GAGNNNNNCTC 2 cut(s) 232, 264
BsaI GGTCTC 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 81, 151
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 115, 351, 474, 515
Bse21I CCTNAGG 1 cut(s) 384
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 2 cut(s) 81, 151
BseGI GGATG 2 cut(s) 464, 484
BseLI CCNNNNNNNGG 5 cut(s) 94, 115, 351, 474, 515
BseMII CTCAG 2 cut(s) 124, 375
BseRI GAGGAG 1 cut(s) 33
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 2 cut(s) 291, 313
BshFI GGCC 1 cut(s) 382
BshNI GGYRCC 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 150
BslI CCNNNNNNNGG 5 cut(s) 94, 115, 351, 474, 515
BsmAI GTCTC 4 cut(s) 15, 40, 235, 465
BsmBI CGTCTC 1 cut(s) 15
BsnI GGCC 1 cut(s) 382
Bso31I GGTCTC 1 cut(s) 40
Bsp1286I GDGCHC 3 cut(s) 150, 158, 490
Bsp143I GATC 2 cut(s) 35, 362
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 2 cut(s) 123, 376
BspHI TCATGA 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 487
BspPI GGATC 1 cut(s) 30
BspQI GCTCTTC 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 81, 151
BssMI GATC 2 cut(s) 35, 362
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 152
Bst6I CTCTTC 1 cut(s) 163
BstDEI CTNAG 4 cut(s) 110, 251, 384, 398
BstF5I GGATG 2 cut(s) 464, 484
BstKTI GATC 2 cut(s) 38, 365
BstMAI GTCTC 4 cut(s) 15, 40, 235, 465
BstMBI GATC 2 cut(s) 35, 362
BstMWI GCNNNNNNNGC 2 cut(s) 145, 301
BstNI CCWGG 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 150
BstSLI GKGCMC 1 cut(s) 490
BstV1I GCAGC 2 cut(s) 291, 313
BstX2I RGATCY 1 cut(s) 362
BstYI RGATCY 1 cut(s) 362
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 1 cut(s) 382
BtsCI GGATG 2 cut(s) 464, 484
CciI TCATGA 1 cut(s) 270
Cfr13I GGNCC 2 cut(s) 85, 506
CviAII CATG 4 cut(s) 121, 171, 271, 491
CviJI RGCY 5 cut(s) 148, 156, 226, 304, 382
CviKI_1 RGCY 5 cut(s) 148, 156, 226, 304, 382
DdeI CTNAG 4 cut(s) 110, 251, 384, 398
DpnI GATC 2 cut(s) 37, 364
DpnII GATC 2 cut(s) 35, 362
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 1 cut(s) 382
Eco24I GRGCYC 2 cut(s) 150, 158
Eco31I GGTCTC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 85, 506
Eco53kI GAGCTC 1 cut(s) 148
Eco81I CCTNAGG 1 cut(s) 384
EcoICRI GAGCTC 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 150
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 2 cut(s) 150, 158
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 4 cut(s) 124, 174, 274, 494
FaiI YATR 7 cut(s) 122, 165, 167, 172, 257, 272, 492
FatI CATG 4 cut(s) 120, 170, 270, 490
FauNDI CATATG 1 cut(s) 165
Fnu4HI GCNGC 2 cut(s) 302, 305
FokI GGATG 2 cut(s) 451, 491
FriOI GRGCYC 2 cut(s) 150, 158
Fsp4HI GCNGC 2 cut(s) 302, 305
FspBI CTAG 1 cut(s) 215
GluI GCNGC 2 cut(s) 302, 305
HaeIII GGCC 1 cut(s) 382
Hin1II CATG 4 cut(s) 124, 174, 274, 494
HinfI GANTC 2 cut(s) 98, 319
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 2 cut(s) 113, 459
Hpy188III TCNNGA 3 cut(s) 271, 345, 468
Hpy8I GTNNAC 1 cut(s) 496
HpyAV CCTTC 9 cut(s) 169, 178, 196, 372, 404, 508, 523, 538, 565
HpyCH4IV ACGT 3 cut(s) 315, 328, 454
HpyCH4V TGCA 2 cut(s) 4, 307
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 301
HpyF3I CTNAG 4 cut(s) 110, 251, 384, 398
HpySE526I ACGT 3 cut(s) 315, 328, 454
Hsp92II CATG 4 cut(s) 124, 174, 274, 494
Kzo9I GATC 2 cut(s) 35, 362
LguI GCTCTTC 1 cut(s) 163
LmnI GCTCC 2 cut(s) 153, 292
LpnPI CCDG 8 cut(s) 137, 164, 274, 358, 396, 460, 481, 489
Lsp1109I GCAGC 2 cut(s) 291, 313
MaeI CTAG 1 cut(s) 215
MaeII ACGT 3 cut(s) 315, 328, 454
MaeIII GTNAC 2 cut(s) 413, 450
MalI GATC 2 cut(s) 37, 364
MboI GATC 2 cut(s) 35, 362
MboII GAAGA 2 cut(s) 150, 286
MflI RGATCY 1 cut(s) 362
MhlI GDGCHC 3 cut(s) 150, 158, 490
MluCI AATT 1 cut(s) 92
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 2 cut(s) 171, 482
MseI TTAA 1 cut(s) 592
MspA1I CMGCKG 1 cut(s) 304
MspR9I CCNGG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 2 cut(s) 145, 301
NdeI CATATG 1 cut(s) 165
NdeII GATC 2 cut(s) 35, 362
NlaIII CATG 4 cut(s) 124, 174, 274, 494
NlaIV GGNNCC 1 cut(s) 487
PagI TCATGA 1 cut(s) 270
PceI AGGCCT 1 cut(s) 382
PciSI GCTCTTC 1 cut(s) 163
PfeI GAWTC 1 cut(s) 319
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 2 cut(s) 303, 306
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 487
PspPI GGNCC 2 cut(s) 85, 506
PsuI RGATCY 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 304
SacI GAGCTC 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 163
SaqAI TTAA 1 cut(s) 592
SatI GCNGC 2 cut(s) 302, 305
Sau3AI GATC 2 cut(s) 35, 362
Sau96I GGNCC 2 cut(s) 85, 506
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 3 cut(s) 150, 158, 490
SinI GGWCC 2 cut(s) 85, 506
Sse9I AATT 1 cut(s) 92
SseBI AGGCCT 1 cut(s) 382
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 150
StuI AGGCCT 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 150
StyI CCWWGG 1 cut(s) 81
TaiI ACGT 3 cut(s) 318, 331, 457
TaqI TCGA 2 cut(s) 101, 356
TasI AATT 1 cut(s) 92
TfiI GAWTC 1 cut(s) 319
Tru1I TTAA 1 cut(s) 592
Tru9I TTAA 1 cut(s) 592
TseI GCWGC 2 cut(s) 301, 304
TspDTI ATGAA 1 cut(s) 287
TspGWI ACGGA 1 cut(s) 131
Van91I CCANNNNNTGG 1 cut(s) 94
VpaK11BI GGWCC 2 cut(s) 85, 506
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.