MD12G1010900.v1.1

Domain of unknown function (DUF4602)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007194
Physical Location & Seq
Reverse (-)
1135796 .. 1138633
2838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1010900.v1.1.491

Sequence Viewer

Length: 534 bp
ATGCAGAAGAATAAGGGAAGACAAAGAGGAGGTGGACCATTTACGAGACGAACCGATAACAAGGAGGAAAACACAGACACCCACTTCCAAAAAATGGACTTGAGGAACATTGCTAAAGAGATTGAGCTTTTGGGTACATCTAATATGACATGGAAGGAGAGGAAGGAGTTGGAAAATAGGAAGGTGGTTTCTCTAGGCGGAAAGCCTCAAAAGAACCGGCGACTACCTCTAAGTGTAGCAAAACTGGTCATGAAGAATCAAAAGAAAAGGGAGCAAAAACTGCAAGAACAGAACATGGTCCTTGGACGTTTTGGTGGGAATTCGAGTGGCAGTTCTAAGAGATCCACGGAGAGGAAGAGGCCCGAGAACAGGGGTTTGAAAGCAAGTGAAGGACACTTTAAAAATGGTGTACTTGATGTGAAGCATTTGCTACGTCCTACTTCATCCACATATGCTGGTCCGAGTATGCTCAGAGGGAAAACTAAGGGGAAGCGAAAGAACAACACAGGTAAGAAGATCGGTGGTATGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4602

Protein Analysis

178

Amino Acids

20.07

Weight (kDa)

10.98

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSAF1 PF15375 15 - 120 1.9e-14 40S small subunit processome assembly factor 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 94
AciI CCGC 1 cut(s) 198
AclWI GGATC 1 cut(s) 336
AcsI RAATTY 1 cut(s) 319
AfaI GTAC 2 cut(s) 136, 411
AfiI CCNNNNNNNGG 3 cut(s) 94, 351, 369
AgsI TTSAA 1 cut(s) 379
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 1 cut(s) 336
Ama87I CYCGRG 1 cut(s) 362
AoxI GGCC 1 cut(s) 359
ApoI RAATTY 1 cut(s) 319
AspS9I GGNCC 4 cut(s) 35, 298, 360, 458
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 3 cut(s) 35, 298, 458
BbsI GAAGAC 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 2 cut(s) 194, 532
Bme18I GGWCC 3 cut(s) 35, 298, 458
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 4 cut(s) 35, 298, 360, 458
BpiI GAAGAC 1 cut(s) 25
BpuEI CTTGAG 1 cut(s) 121
BsaJI CCNNGG 2 cut(s) 301, 345
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 351, 369
Bse118I RCCGGY 1 cut(s) 216
Bse1I ACTGG 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 301, 345
BseGI GGATG 1 cut(s) 443
BseLI CCNNNNNNNGG 3 cut(s) 94, 351, 369
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 484
BseNI ACTGG 1 cut(s) 249
BseRI GAGGAG 1 cut(s) 42
BshFI GGCC 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 1 cut(s) 217
BslI CCNNNNNNNGG 3 cut(s) 94, 351, 369
BsmAI GTCTC 1 cut(s) 40
BsmBI CGTCTC 1 cut(s) 40
BsnI GGCC 1 cut(s) 361
BsoBI CYCGRG 1 cut(s) 362
Bsp143I GATC 2 cut(s) 341, 516
BspACI CCGC 1 cut(s) 198
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 1 cut(s) 483
BspHI TCATGA 1 cut(s) 249
BspPI GGATC 1 cut(s) 336
BsrDI GCAATG 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 216
BsrI ACTGG 1 cut(s) 249
BssAI RCCGGY 1 cut(s) 216
BssECI CCNNGG 2 cut(s) 301, 345
BssMI GATC 2 cut(s) 341, 516
BssT1I CCWWGG 1 cut(s) 301
Bst6I CTCTTC 1 cut(s) 350
BstAPI GCANNNNNTGC 1 cut(s) 280
BstDEI CTNAG 4 cut(s) 230, 336, 470, 483
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 1 cut(s) 443
BstKTI GATC 2 cut(s) 344, 519
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 2 cut(s) 341, 516
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 341
BstYI RGATCY 1 cut(s) 341
BsuRI GGCC 1 cut(s) 361
BtgI CCRYGG 1 cut(s) 345
BtsCI GGATG 1 cut(s) 443
CciI TCATGA 1 cut(s) 249
Cfr10I RCCGGY 1 cut(s) 216
Cfr13I GGNCC 4 cut(s) 35, 298, 360, 458
Csp6I GTAC 2 cut(s) 135, 410
CviAII CATG 3 cut(s) 150, 250, 295
CviJI RGCY 3 cut(s) 127, 205, 361
CviKI_1 RGCY 3 cut(s) 127, 205, 361
CviQI GTAC 2 cut(s) 135, 410
DdeI CTNAG 4 cut(s) 230, 336, 470, 483
DpnI GATC 2 cut(s) 343, 518
DpnII GATC 2 cut(s) 341, 516
DraI TTTAAA 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
EciI GGCGGA 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 301
Eco47I GGWCC 3 cut(s) 35, 298, 458
Eco88I CYCGRG 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 301
Esp3I CGTCTC 1 cut(s) 40
FaeI CATG 3 cut(s) 153, 253, 298
FaiI YATR 8 cut(s) 146, 151, 251, 296, 451, 453, 467, 527
FatI CATG 3 cut(s) 149, 249, 294
FauNDI CATATG 1 cut(s) 451
FokI GGATG 1 cut(s) 430
FspBI CTAG 2 cut(s) 194, 532
HaeIII GGCC 1 cut(s) 361
HapII CCGG 1 cut(s) 217
Hin1II CATG 3 cut(s) 153, 253, 298
HinfI GANTC 1 cut(s) 256
HpaII CCGG 1 cut(s) 217
Hpy166II GTNNAC 2 cut(s) 35, 410
Hpy188I TCNGA 2 cut(s) 462, 473
Hpy188III TCNNGA 1 cut(s) 250
Hpy8I GTNNAC 2 cut(s) 35, 410
HpyAV CCTTC 4 cut(s) 148, 157, 175, 383
HpyCH4IV ACGT 2 cut(s) 307, 433
HpyCH4V TGCA 2 cut(s) 4, 283
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpyF3I CTNAG 4 cut(s) 230, 336, 470, 483
HpySE526I ACGT 2 cut(s) 307, 433
Hsp92II CATG 3 cut(s) 153, 253, 298
Kzo9I GATC 2 cut(s) 341, 516
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 5 cut(s) 230, 230, 355, 441, 492
MaeI CTAG 2 cut(s) 194, 532
MaeII ACGT 2 cut(s) 307, 433
MalI GATC 2 cut(s) 343, 518
MboI GATC 2 cut(s) 341, 516
MboII GAAGA 5 cut(s) 19, 30, 265, 367, 526
MflI RGATCY 1 cut(s) 341
MluCI AATT 1 cut(s) 319
MmeI TCCRAC 1 cut(s) 150
MseI TTAA 1 cut(s) 399
MspI CCGG 1 cut(s) 217
MwoI GCNNNNNNNGC 1 cut(s) 280
NdeI CATATG 1 cut(s) 451
NdeII GATC 2 cut(s) 341, 516
NlaIII CATG 3 cut(s) 153, 253, 298
PagI TCATGA 1 cut(s) 249
PfeI GAWTC 1 cut(s) 256
PflMI CCANNNNNTGG 1 cut(s) 94
PspPI GGNCC 4 cut(s) 35, 298, 360, 458
PsuI RGATCY 1 cut(s) 341
RsaI GTAC 2 cut(s) 136, 411
RsaNI GTAC 2 cut(s) 135, 410
SaqAI TTAA 1 cut(s) 399
Sau3AI GATC 2 cut(s) 341, 516
Sau96I GGNCC 4 cut(s) 35, 298, 360, 458
SetI ASST 7 cut(s) 34, 129, 186, 229, 310, 436, 511
SinI GGWCC 3 cut(s) 35, 298, 458
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
Sse9I AATT 1 cut(s) 319
SsiI CCGC 1 cut(s) 198
SspMI CTAG 2 cut(s) 194, 532
StyI CCWWGG 1 cut(s) 301
TaiI ACGT 2 cut(s) 310, 436
TaqI TCGA 1 cut(s) 323
TasI AATT 1 cut(s) 319
TatI WGTACW 1 cut(s) 409
TfiI GAWTC 1 cut(s) 256
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TspDTI ATGAA 2 cut(s) 266, 432
TspGWI ACGGA 1 cut(s) 362
Van91I CCANNNNNTGG 1 cut(s) 94
VpaK11BI GGWCC 3 cut(s) 35, 298, 458
XapI RAATTY 1 cut(s) 319
XspI CTAG 2 cut(s) 194, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.