FvH4_3g25880

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003192
Physical Location & Seq
Reverse (-)
18717493 .. 18723635
6143 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25880.t1

Sequence Viewer

Length: 1191 bp
ATGGTCTACTCAGTTGTATCCGGGGATACTTCTATAGAAAATTGCTATGCTCACCGATTGGAGTTCAGCACATGTTCTTGGGATGAGTTTGCTGATGCTAATCAATGTGTTTCTGGTCCATCAAAAGCTACTTCTGAAGTAGTCTGTTCATTTGCTTCTGGAGAAAATGCTGCAGCGGAAATTGGAGTTCCGTCCTTCAGTGGACACAGAAGGTCTACTTTCTTCATTCAGCAGTGTCCTATCAGTTGCAGAAGGCACTTCAAGAATTACGGAGACCATGCCTCTCACACTACTTGTGGAAAACAGCACGTCAAGATCTGTGAGTCCGTTCAACATGAGGCTGCAATAACCTCTTCAGATACTGGACGTTTGGATGAATCAAGTTCTGATATTGCTTACTGTCACATGGAAAACATTGACTTGCATGATAAGGTGCAGATTCAGGAAAACTGTGTAAATGTAGAGGATAGTGTACTTAGTGCTATTTCTCACAGAATAAGAAAGCAAAGATCATACAAGAAACAAATCCAGGATGCTTTTTCATCAAAGAAGAGGTTGGTGAAGGAGTACGAACAGCTAGCGATTTGGTTTGGAGATGTTGGTTTAGATTCCAGCCAAGAAGCAAACTCTTACAAGGACAATTCGGGAACTCATCAGGCAGCGCTCTGGTGGGGTTTTTGGGATTCAAGGAAGACTTGGATAAGGAACAGGAGTCGAATCTGGAGCTGTAGTCATGCTTTGACTACTCTACAGAGGCTGGAAGAAGAGGAGAGGAGAGGCAGAATAGCCATTAATGGAGTATTAGAGCTCAGTTGCGTAAGGTTCATTCATCAATACGTGGTGGAAGAACAATTCTTTTATAGAGCCGCATATGAGAAGTCAGTTTTCTGCGATATGTTTCATTTAGGGGAAACGGGTTGGAGGGACTGCAGTTTGTGCCACAAGCCTCTCCACTGTGGATGCGTAGCCTCTAGGTCTTTATATGAGTGCCTGGATTACGGGGGTGTAGGGTGCATTGGCTGTGCAAACCGTTCTCAACCTCGTGTGGGTGTGTTGAACTTAATTTTAGCCGGAACTTGCCTGCAGAGTAGAGAACATTGTGTGTGCTCTGGATCCTGGCTGCAAGGAGCAGCTGCCACAGTTACCACCAAAAATACGGTGAAAGGGTATTCAAGCGTGCATCACGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

397

Amino Acids

44.49

Weight (kDa)

6.67

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 299 - 346 3.3e-22 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 6, 215
AciI CCGC 2 cut(s) 176, 867
AclWI GGATC 2 cut(s) 1107, 1120
AcuI CTGAAG 3 cut(s) 156, 181, 339
AfaI GTAC 2 cut(s) 474, 569
AfeI AGCGCT 1 cut(s) 663
AfiI CCNNNNNNNGG 1 cut(s) 1046
AflIII ACRYGT 1 cut(s) 71
AgsI TTSAA 5 cut(s) 262, 332, 687, 1057, 1173
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 3 cut(s) 528, 990, 1115
AluBI AGCT 5 cut(s) 128, 577, 726, 808, 1133
AluI AGCT 5 cut(s) 128, 577, 726, 808, 1133
Alw21I GWGCWC 2 cut(s) 810, 1109
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 2 cut(s) 1107, 1120
AlwNI CAGNNNCTG 2 cut(s) 362, 757
Aor51HI AGCGCT 1 cut(s) 663
ApeKI GCWGC 7 cut(s) 170, 173, 341, 659, 1120, 1130, 1133
AseI ATTAAT 1 cut(s) 792
AspLEI GCGC 1 cut(s) 664
AspS9I GGNCC 1 cut(s) 116
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 3 cut(s) 44, 571, 1171
AsuNHI GCTAGC 1 cut(s) 577
AvaII GGWCC 1 cut(s) 116
BamHI GGATCC 1 cut(s) 1112
BanII GRGCYC 1 cut(s) 810
BauI CACGAG 1 cut(s) 1041
BbsI GAAGAC 1 cut(s) 698
Bbv12I GWGCWC 2 cut(s) 810, 1109
BbvI GCAGC 7 cut(s) 157, 185, 328, 671, 1107, 1120, 1142
BccI CCATC 1 cut(s) 127
BciT130I CCWGG 3 cut(s) 530, 992, 1117
BciVI GTATCC 2 cut(s) 19, 28
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 2 cut(s) 578, 972
BfmI CTRYAG 6 cut(s) 33, 171, 727, 749, 928, 1082
BfoI RGCGCY 1 cut(s) 665
BfuI GTATCC 2 cut(s) 19, 28
BglII AGATCT 1 cut(s) 315
BisI GCNGC 8 cut(s) 171, 174, 342, 660, 867, 1121, 1131, 1134
BlsI GCNGC 8 cut(s) 172, 175, 343, 661, 868, 1122, 1132, 1135
Bme1390I CCNGG 4 cut(s) 22, 530, 992, 1117
Bme18I GGWCC 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 1114
BmrFI CCNGG 4 cut(s) 22, 530, 992, 1117
BmsI GCATC 3 cut(s) 85, 523, 950
BmtI GCTAGC 1 cut(s) 581
BpiI GAAGAC 1 cut(s) 698
BpmI CTGGAG 2 cut(s) 180, 742
BpuMI CCSGG 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 838
BsaBI GATNNNNATC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 715, 745
Bsc4I CCNNNNNNNGG 1 cut(s) 1046
Bse1I ACTGG 1 cut(s) 367
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 3 cut(s) 530, 992, 1117
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 4 cut(s) 88, 379, 538, 965
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 1046
BseMII CTCAG 2 cut(s) 24, 823
BseNI ACTGG 1 cut(s) 367
BseRI GAGGAG 2 cut(s) 782, 787
BseXI GCAGC 7 cut(s) 157, 185, 328, 671, 1107, 1120, 1142
BsgI GTGCAG 1 cut(s) 455
BsiHKAI GWGCWC 2 cut(s) 810, 1109
BsiSI CCGG 2 cut(s) 21, 1071
BslFI GGGAC 1 cut(s) 938
BslI CCNNNNNNNGG 1 cut(s) 1046
BsmAI GTCTC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 938
Bso31I GGTCTC 1 cut(s) 267
Bsp1286I GDGCHC 2 cut(s) 810, 1109
Bsp143I GATC 3 cut(s) 315, 509, 1112
BspACI CCGC 2 cut(s) 176, 867
BspCNI CTCAG 2 cut(s) 23, 822
BspLI GGNNCC 1 cut(s) 1114
BspMAI CTGCAG 3 cut(s) 175, 932, 1086
BspOI GCTAGC 1 cut(s) 581
BspPI GGATC 2 cut(s) 1107, 1120
BspTNI GGTCTC 1 cut(s) 267
BsrI ACTGG 1 cut(s) 367
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 3 cut(s) 315, 509, 1112
BssSI CACGAG 1 cut(s) 1041
Bst2BI CACGAG 1 cut(s) 1041
Bst2UI CCWGG 3 cut(s) 530, 992, 1117
Bst4CI ACNGT 6 cut(s) 401, 452, 956, 1031, 1141, 1159
Bst6I CTCTTC 3 cut(s) 358, 545, 759
BstAPI GCANNNNNTGC 1 cut(s) 936
BstBAI YACGTR 1 cut(s) 838
BstC8I GCNNGC 3 cut(s) 579, 1082, 1178
BstDEI CTNAG 3 cut(s) 10, 476, 809
BstF5I GGATG 4 cut(s) 88, 379, 538, 965
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 1 cut(s) 664
BstKTI GATC 3 cut(s) 318, 512, 1115
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 3 cut(s) 315, 509, 1112
BstMWI GCNNNNNNNGC 1 cut(s) 936
BstNI CCWGG 3 cut(s) 530, 992, 1117
BstNSI RCATGY 1 cut(s) 75
BstSCI CCNGG 4 cut(s) 20, 528, 990, 1115
BstSFI CTRYAG 6 cut(s) 33, 171, 727, 749, 928, 1082
BstV1I GCAGC 7 cut(s) 157, 185, 328, 671, 1107, 1120, 1142
BstV2I GAAGAC 1 cut(s) 698
BstX2I RGATCY 2 cut(s) 315, 1112
BstYI RGATCY 2 cut(s) 315, 1112
BsuI GTATCC 2 cut(s) 19, 28
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 4 cut(s) 88, 379, 538, 965
BtsI GCAGTG 1 cut(s) 239
BtsIMutI CAGTG 3 cut(s) 205, 239, 952
Cac8I GCNNGC 3 cut(s) 579, 1082, 1178
CaiI CAGNNNCTG 2 cut(s) 362, 757
CfoI GCGC 1 cut(s) 664
Cfr13I GGNCC 1 cut(s) 116
Csp6I GTAC 2 cut(s) 473, 568
CviAII CATG 6 cut(s) 72, 278, 335, 406, 425, 734
CviQI GTAC 2 cut(s) 473, 568
DdeI CTNAG 3 cut(s) 10, 476, 809
DpnI GATC 3 cut(s) 317, 511, 1114
DpnII GATC 3 cut(s) 315, 509, 1112
Eam1104I CTCTTC 3 cut(s) 358, 545, 759
EarI CTCTTC 3 cut(s) 358, 545, 759
Ecl136II GAGCTC 1 cut(s) 808
Eco24I GRGCYC 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 116
Eco47III AGCGCT 1 cut(s) 663
Eco53kI GAGCTC 1 cut(s) 808
Eco57I CTGAAG 3 cut(s) 156, 181, 339
EcoICRI GAGCTC 1 cut(s) 808
EcoRII CCWGG 3 cut(s) 528, 990, 1115
EcoT38I GRGCYC 1 cut(s) 810
FaeI CATG 6 cut(s) 75, 281, 338, 409, 428, 737
FalI AAGNNNNNCTT 4 cut(s) 202, 234, 679, 711
FaqI GGGAC 1 cut(s) 938
FatI CATG 6 cut(s) 71, 277, 334, 405, 424, 733
FauNDI CATATG 1 cut(s) 871
FblI GTMKAC 2 cut(s) 6, 215
Fnu4HI GCNGC 8 cut(s) 171, 174, 342, 660, 867, 1121, 1131, 1134
FokI GGATG 4 cut(s) 95, 386, 545, 972
FriOI GRGCYC 1 cut(s) 810
Fsp4HI GCNGC 8 cut(s) 171, 174, 342, 660, 867, 1121, 1131, 1134
FspBI CTAG 2 cut(s) 578, 972
GlaI GCGC 1 cut(s) 663
GluI GCNGC 8 cut(s) 171, 174, 342, 660, 867, 1121, 1131, 1134
GsuI CTGGAG 2 cut(s) 180, 742
HaeII RGCGCY 1 cut(s) 665
HapII CCGG 2 cut(s) 21, 1071
HhaI GCGC 1 cut(s) 664
Hin1II CATG 6 cut(s) 75, 281, 338, 409, 428, 737
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HinfI GANTC 7 cut(s) 323, 377, 439, 608, 683, 712, 717
HpaII CCGG 2 cut(s) 21, 1071
HphI GGTGA 3 cut(s) 44, 571, 1171
Hpy166II GTNNAC 4 cut(s) 7, 203, 216, 473
Hpy188I TCNGA 3 cut(s) 136, 358, 388
Hpy188III TCNNGA 8 cut(s) 159, 262, 313, 443, 645, 721, 1110, 1184
Hpy8I GTNNAC 4 cut(s) 7, 203, 216, 473
HpyAV CCTTC 4 cut(s) 204, 205, 246, 556
HpyCH4III ACNGT 6 cut(s) 401, 452, 956, 1031, 1141, 1159
HpyCH4IV ACGT 3 cut(s) 309, 367, 837
HpyF10VI GCNNNNNNNGC 1 cut(s) 936
HpyF3I CTNAG 3 cut(s) 10, 476, 809
HpySE526I ACGT 3 cut(s) 309, 367, 837
Hsp92II CATG 6 cut(s) 75, 281, 338, 409, 428, 737
HspAI GCGC 1 cut(s) 662
Kzo9I GATC 3 cut(s) 315, 509, 1112
LmnI GCTCC 2 cut(s) 723, 1127
Lsp1109I GCAGC 7 cut(s) 157, 185, 328, 671, 1107, 1120, 1142
LweI GCATC 3 cut(s) 85, 523, 950
MaeI CTAG 2 cut(s) 578, 972
MaeII ACGT 3 cut(s) 309, 367, 837
MaeIII GTNAC 2 cut(s) 401, 1141
MalI GATC 3 cut(s) 317, 511, 1114
MboI GATC 3 cut(s) 315, 509, 1112
MboII GAAGA 7 cut(s) 214, 345, 562, 703, 773, 776, 857
MflI RGATCY 2 cut(s) 315, 1112
MhlI GDGCHC 2 cut(s) 810, 1109
MluCI AATT 6 cut(s) 40, 180, 265, 640, 851, 1062
MlyI GAGTC 2 cut(s) 332, 721
MmeI TCCRAC 1 cut(s) 899
MseI TTAA 2 cut(s) 792, 1061
MspA1I CMGCKG 2 cut(s) 176, 1133
MspI CCGG 2 cut(s) 21, 1071
MspR9I CCNGG 4 cut(s) 22, 530, 992, 1117
MvaI CCWGG 3 cut(s) 530, 992, 1117
MwoI GCNNNNNNNGC 1 cut(s) 936
NciI CCSGG 1 cut(s) 22
NdeI CATATG 1 cut(s) 871
NdeII GATC 3 cut(s) 315, 509, 1112
NheI GCTAGC 1 cut(s) 577
NlaIII CATG 6 cut(s) 75, 281, 338, 409, 428, 737
NlaIV GGNNCC 1 cut(s) 1114
NmuCI GTSAC 1 cut(s) 401
NspI RCATGY 1 cut(s) 75
PciI ACATGT 1 cut(s) 71
PfeI GAWTC 5 cut(s) 377, 439, 608, 683, 717
PfoI TCCNGGA 1 cut(s) 528
PkrI GCNGC 8 cut(s) 172, 175, 343, 661, 868, 1122, 1132, 1135
PleI GAGTC 2 cut(s) 331, 720
PpsI GAGTC 2 cut(s) 331, 720
Ppu21I YACGTR 1 cut(s) 838
PscI ACATGT 1 cut(s) 71
PshBI ATTAAT 1 cut(s) 792
Psp124BI GAGCTC 1 cut(s) 810
Psp6I CCWGG 3 cut(s) 528, 990, 1115
PspGI CCWGG 3 cut(s) 528, 990, 1115
PspN4I GGNNCC 1 cut(s) 1114
PspPI GGNCC 1 cut(s) 116
PstI CTGCAG 3 cut(s) 175, 932, 1086
PstNI CAGNNNCTG 2 cut(s) 362, 757
PsuI RGATCY 2 cut(s) 315, 1112
PvuII CAGCTG 1 cut(s) 1133
RsaI GTAC 2 cut(s) 474, 569
RsaNI GTAC 2 cut(s) 473, 568
SacI GAGCTC 1 cut(s) 810
SaqAI TTAA 2 cut(s) 792, 1061
SatI GCNGC 8 cut(s) 171, 174, 342, 660, 867, 1121, 1131, 1134
Sau3AI GATC 3 cut(s) 315, 509, 1112
Sau96I GGNCC 1 cut(s) 116
SchI GAGTC 2 cut(s) 332, 721
ScrFI CCNGG 4 cut(s) 22, 530, 992, 1117
SduI GDGCHC 2 cut(s) 810, 1109
SfaNI GCATC 3 cut(s) 85, 523, 950
SfcI CTRYAG 6 cut(s) 33, 171, 727, 749, 928, 1082
SinI GGWCC 1 cut(s) 116
Sse9I AATT 6 cut(s) 40, 180, 265, 640, 851, 1062
SsiI CCGC 2 cut(s) 176, 867
SspMI CTAG 2 cut(s) 578, 972
SstI GAGCTC 1 cut(s) 810
StyD4I CCNGG 4 cut(s) 20, 528, 990, 1115
TaaI ACNGT 6 cut(s) 401, 452, 956, 1031, 1141, 1159
TaiI ACGT 3 cut(s) 312, 370, 840
TaqI TCGA 1 cut(s) 715
TasI AATT 6 cut(s) 40, 180, 265, 640, 851, 1062
TatI WGTACW 1 cut(s) 472
TauI GCSGC 1 cut(s) 869
TfiI GAWTC 5 cut(s) 377, 439, 608, 683, 717
Tru1I TTAA 2 cut(s) 792, 1061
Tru9I TTAA 2 cut(s) 792, 1061
TscAI CASTG 3 cut(s) 205, 239, 959
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 7 cut(s) 170, 173, 341, 659, 1120, 1130, 1133
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 7 cut(s) 138, 214, 390, 531, 814, 818, 890
TspGWI ACGGA 3 cut(s) 180, 285, 316
TspRI CASTG 3 cut(s) 205, 239, 959
VpaK11BI GGWCC 1 cut(s) 116
VspI ATTAAT 1 cut(s) 792
XceI RCATGY 1 cut(s) 75
XmiI GTMKAC 2 cut(s) 6, 215
XspI CTAG 2 cut(s) 578, 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.