RLG00000002875

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003192
Physical Location & Seq
Forward (+)
40995468 .. 40998058
2591 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002875

Sequence Viewer

Length: 543 bp
ATGCTCGTCATGCGGAAGTTCTGGCAGTGGTGCTTCAAGCTGGAGTACCTTAATAGCTTGTTCAGCTTTTGGCCATCTGTTGCTGTTGGGGTTCAGTGGGGCGAAGATCTACCGTGGCAGAGGCATCAGATTTATCCTGTTCGACTCTCAGGGATGAAGATGATCGCTGCATATTTGCTCGTTGTGTTGGGTGGCAAGATCAGCTCTACTGTCGAAGACATCCTTGGCCCCGGGAGATACGTGGCTCCTGAAGTTTACAGAAATGAAGAATATGATACCAAAGTGGATGATTTTTCATTTTCCTTGATCTTGCAAGAGGATACAATTAAATATGTGGAAAACCAGATGCATTCAGTGGGTAAAAATGTGAATAAACTCTGTTCTGATTTACTTCCTCCTTTAGGTCATTCTGTTAAACATGTGGCTGAAGATGTGGTTATTAAAAAGAATGCTACTGTTAGTACTAAGACAAGCAAAAGCTTGCAGGAAAATTCTGTTGATGCAACTGAAAAAAATTCTTCTGAAATTTTCAATTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.6

Weight (kDa)

6.09

Isoelectric Point (pI)

33.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 13
AcoI YGGCCR 1 cut(s) 71
AcsI RAATTY 3 cut(s) 490, 514, 525
AcuI CTGAAG 2 cut(s) 270, 447
AfaI GTAC 2 cut(s) 47, 463
AfiI CCNNNNNNNGG 1 cut(s) 401
AflIII ACRYGT 1 cut(s) 418
AgsI TTSAA 2 cut(s) 37, 532
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
AluBI AGCT 5 cut(s) 40, 57, 66, 204, 480
AluI AGCT 5 cut(s) 40, 57, 66, 204, 480
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 2 cut(s) 71, 226
ApeKI GCWGC 1 cut(s) 167
ApoI RAATTY 3 cut(s) 490, 514, 525
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 2 cut(s) 231, 232
AvaI CYCGRG 1 cut(s) 230
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BalI TGGCCA 1 cut(s) 73
BbsI GAAGAC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 154
BccI CCATC 1 cut(s) 82
BciVI GTATCC 1 cut(s) 313
BcnI CCSGG 2 cut(s) 231, 232
BfuI GTATCC 1 cut(s) 313
BglII AGATCT 1 cut(s) 106
BisI GCNGC 1 cut(s) 168
BlsI GCNGC 1 cut(s) 169
BmcAI AGTACT 1 cut(s) 463
Bme1390I CCNGG 2 cut(s) 231, 232
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 2 cut(s) 229, 246
BmrFI CCNGG 2 cut(s) 231, 232
BmsI GCATC 3 cut(s) 133, 336, 490
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 62
BpuMI CCSGG 2 cut(s) 231, 232
BsaAI YACGTR 1 cut(s) 241
BsaJI CCNNGG 4 cut(s) 113, 223, 229, 230
Bsc4I CCNNNNNNNGG 1 cut(s) 401
BseDI CCNNGG 4 cut(s) 113, 223, 229, 230
BseGI GGATG 3 cut(s) 159, 219, 292
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMII CTCAG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 154
BshFI GGCC 2 cut(s) 73, 228
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 1 cut(s) 231
BslI CCNNNNNNNGG 1 cut(s) 401
BsmI GAATGC 2 cut(s) 349, 454
BsnI GGCC 2 cut(s) 73, 228
BsoBI CYCGRG 1 cut(s) 230
Bsp143I GATC 4 cut(s) 106, 162, 198, 306
BspACI CCGC 1 cut(s) 13
BspANI GGCC 2 cut(s) 73, 228
BspCNI CTCAG 1 cut(s) 161
BspLI GGNNCC 2 cut(s) 229, 246
BssECI CCNNGG 4 cut(s) 113, 223, 229, 230
BssMI GATC 4 cut(s) 106, 162, 198, 306
BssT1I CCWWGG 1 cut(s) 223
Bst4CI ACNGT 3 cut(s) 114, 211, 457
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 482
BstDEI CTNAG 2 cut(s) 148, 465
BstDSI CCRYGG 1 cut(s) 113
BstENI CCTNNNNNAGG 1 cut(s) 399
BstF5I GGATG 3 cut(s) 159, 219, 292
BstKTI GATC 4 cut(s) 109, 165, 201, 309
BstMBI GATC 4 cut(s) 106, 162, 198, 306
BstMWI GCNNNNNNNGC 3 cut(s) 10, 63, 201
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 2 cut(s) 229, 230
BstV1I GCAGC 1 cut(s) 154
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 2 cut(s) 73, 228
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 3 cut(s) 159, 219, 292
BtsI GCAGTG 1 cut(s) 32
BtsIMutI CAGTG 3 cut(s) 32, 101, 360
Cac8I GCNNGC 1 cut(s) 482
Cfr13I GGNCC 1 cut(s) 227
Cfr9I CCCGGG 1 cut(s) 230
Csp6I GTAC 2 cut(s) 46, 462
CviAII CATG 2 cut(s) 10, 419
CviJI RGCY 9 cut(s) 40, 57, 66, 73, 204, 228, 245, 425, 480
CviKI_1 RGCY 9 cut(s) 40, 57, 66, 73, 204, 228, 245, 425, 480
CviQI GTAC 2 cut(s) 46, 462
DdeI CTNAG 2 cut(s) 148, 465
DpnI GATC 4 cut(s) 108, 164, 200, 308
DpnII GATC 4 cut(s) 106, 162, 198, 306
EaeI YGGCCR 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 270, 447
Eco88I CYCGRG 1 cut(s) 230
EcoNI CCTNNNNNAGG 1 cut(s) 399
EcoT14I CCWWGG 1 cut(s) 223
EcoT22I ATGCAT 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 223
FaeI CATG 2 cut(s) 13, 422
FaiI YATR 5 cut(s) 11, 172, 273, 333, 420
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FatI CATG 2 cut(s) 9, 418
Fnu4HI GCNGC 1 cut(s) 168
FokI GGATG 3 cut(s) 166, 206, 299
Fsp4HI GCNGC 1 cut(s) 168
GluI GCNGC 1 cut(s) 168
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 2 cut(s) 73, 228
HapII CCGG 1 cut(s) 231
Hin1II CATG 2 cut(s) 13, 422
HindIII AAGCTT 1 cut(s) 478
HinfI GANTC 1 cut(s) 144
HpaII CCGG 1 cut(s) 231
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 4 cut(s) 129, 385, 523, 542
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 1 cut(s) 256
HpyCH4III ACNGT 3 cut(s) 114, 211, 457
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 5 cut(s) 170, 313, 349, 484, 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 63, 201
HpyF3I CTNAG 2 cut(s) 148, 465
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 2 cut(s) 13, 422
Kzo9I GATC 4 cut(s) 106, 162, 198, 306
LmnI GCTCC 1 cut(s) 250
LpnPI CCDG 8 cut(s) 7, 26, 135, 150, 244, 261, 356, 470
Lsp1109I GCAGC 1 cut(s) 154
LweI GCATC 3 cut(s) 133, 336, 490
MaeII ACGT 1 cut(s) 240
MalI GATC 4 cut(s) 108, 164, 200, 308
MboI GATC 4 cut(s) 106, 162, 198, 306
MboII GAAGA 7 cut(s) 116, 169, 227, 278, 440, 510, 529
MflI RGATCY 1 cut(s) 106
MlsI TGGCCA 1 cut(s) 73
MluCI AATT 5 cut(s) 324, 490, 514, 525, 532
MluNI TGGCCA 1 cut(s) 73
MlyI GAGTC 1 cut(s) 138
MnlI CCTC 3 cut(s) 114, 310, 405
Mox20I TGGCCA 1 cut(s) 73
Mph1103I ATGCAT 1 cut(s) 351
MscI TGGCCA 1 cut(s) 73
MseI TTAA 4 cut(s) 51, 327, 414, 441
Msp20I TGGCCA 1 cut(s) 73
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 2 cut(s) 231, 232
Mva1269I GAATGC 2 cut(s) 349, 454
MwoI GCNNNNNNNGC 3 cut(s) 10, 63, 201
NciI CCSGG 2 cut(s) 231, 232
NdeII GATC 4 cut(s) 106, 162, 198, 306
NlaIII CATG 2 cut(s) 13, 422
NlaIV GGNNCC 2 cut(s) 229, 246
NsiI ATGCAT 1 cut(s) 351
NspI RCATGY 1 cut(s) 422
PciI ACATGT 1 cut(s) 418
PctI GAATGC 2 cut(s) 349, 454
PkrI GCNGC 1 cut(s) 169
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
Ppu21I YACGTR 1 cut(s) 241
PscI ACATGT 1 cut(s) 418
PspN4I GGNNCC 2 cut(s) 229, 246
PspPI GGNCC 1 cut(s) 227
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 2 cut(s) 47, 463
RsaNI GTAC 2 cut(s) 46, 462
SaqAI TTAA 4 cut(s) 51, 327, 414, 441
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 4 cut(s) 106, 162, 198, 306
Sau96I GGNCC 1 cut(s) 227
ScaI AGTACT 1 cut(s) 463
SchI GAGTC 1 cut(s) 138
ScrFI CCNGG 2 cut(s) 231, 232
SetI ASST 8 cut(s) 42, 51, 59, 68, 206, 243, 406, 482
SfaNI GCATC 3 cut(s) 133, 336, 490
SmaI CCCGGG 1 cut(s) 232
Sse9I AATT 5 cut(s) 324, 490, 514, 525, 532
SsiI CCGC 1 cut(s) 13
StyD4I CCNGG 2 cut(s) 229, 230
StyI CCWWGG 1 cut(s) 223
TaaI ACNGT 3 cut(s) 114, 211, 457
TaiI ACGT 1 cut(s) 243
TaqI TCGA 2 cut(s) 142, 213
TasI AATT 5 cut(s) 324, 490, 514, 525, 532
TatI WGTACW 1 cut(s) 461
Tru1I TTAA 4 cut(s) 51, 327, 414, 441
Tru9I TTAA 4 cut(s) 51, 327, 414, 441
TscAI CASTG 3 cut(s) 32, 101, 360
TseI GCWGC 1 cut(s) 167
TspDTI ATGAA 3 cut(s) 170, 279, 285
TspMI CCCGGG 1 cut(s) 230
TspRI CASTG 3 cut(s) 32, 101, 360
XagI CCTNNNNNAGG 1 cut(s) 399
XapI RAATTY 3 cut(s) 490, 514, 525
XceI RCATGY 1 cut(s) 422
XmaI CCCGGG 1 cut(s) 230
ZrmI AGTACT 1 cut(s) 463
Zsp2I ATGCAT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.