FvH4_3g27840

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008095
Physical Location & Seq
Forward (+)
20858455 .. 20865650
7196 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27840.t3

Sequence Viewer

Length: 2772 bp
ATGATGATCACTCAGACTGATAAAATGTCTGCTCCTCCATCATGGGAGCGTGCTCAGCGTTTGTATGACAAGATTACTGAGTTGGAGAGCAGGCGTCGAAGGTCTGCCCAAGCGCGAGTTCCATCAGATCCCAATGCCTGGCAACAGATACGTGAGAATTATGAAGCTATAATTCTAGAGGACCATGCATTTTCAGAGCAACACAATGTTGAGTATGCTTTGTGGCAGTTACATTACAAGCGGATCGACGAATTGCGTGCACACTTTAGTGCTGCTATGGCTTCTACAGGTTCAAACTCATCACAAAGTTTGAAGGGTCCTACTCGGCCTGATCGTATTACAAAAATAAGATTGCAGTTTAAAACATTCCTTTCAGAAGCGACTGGATTTTATCATGATTTAATAGTGAAGATCAGAGCAAAGTATGGGCTTCCTCTTGGCTATTTGTCTGAGGATTCTGAAAATCGAATTGTTATGGATAAGGATGGAAAGAAATCTGCTGACATGAAGAAAGGTTTGATATCATGTCACCGCTGTTTGATTTACTTGGGTGACCTTGCACGCTACAAAGGACTGTATGGAGAGGGGGACTCCAAAACTCGCGAGTATGCTGCTGCCTCAAGTTACTACTTGCAAGCTGCATCTTGTTTGCCATCAAGTGGGAATCCTCATCATCAGCTTGCTATACTGGCCTCTTATTCAGGCGATGAGGTGGTCACTGTTTATCGATATTTTCGTAGCCTGGCAGTGGATAATCCCTTTTCAACTGCAAGGGATAACTTGATTGTGGCATTTGAGAAGAACCGCCAGAGTTACTCTCAACTGTGTGGCAATGCTAATGCTTCTGCACTGAAAGAGTTGCCTGCACGTTCGACTGGTAAAGGTAGAGGGAAAGGGGACGCAATACCTGCTAAAGATAACAACACAGAAGCAGGCCTTGTAAAGGAAAGACCATCCAGTAACCAGGAGAAATACAAAGCTTTTTCAATTCGTTTTGTTCGTCTAAATGGCATTCTTTTTACACGCACTAGCCTGGAGACATTTGCAGAAGTGCTCTCTGTTGTAAGCAGTGGTTTAATTGAGCTTTTATCTTCTGGAGCAGAAGAGGAGCTATTTGGTGCTGATGGCATTGAGGATGGACTGGTCATTGTCAGAATGGTCTCTATACTAATATTTACAGTTCATAATGTGAAAAAGGAAAGTGAAGGACAGTCATATGCAGAAATTGTACAACGTGCTGTTCTACTGCAGAATGCATTTACTGCTGTCTTCGAGTTGATGGGGCATGTCTTAGAGAGATGTGTGAAGCTGGGCGATCCAACTTCCAGTTACCTGTTACCAGGCATTCTGGTTTTTGTAGAATGGTTGGCATGCTGTCCCGACCTAGCAGCTGGTAGTGATGCAGATGAAAAACAGTCCTCTGTTAGAGCAAAGTTCTGGAATGCTTGCATACTCTTGTTGAATAATCTCTTGTCATTTGTCCCATTGTCCATAGATGATGATGGAGATGAGACTTGCTTTAATAATATGAGCAGATATGAAGAAGGGGAAACTGAGAATCGCCTTGCTCTATGGGAGGATTTTGAGTTGAGAGGATTTATGCCACTTCTTCCAGCACATACAATATTGGATTTCTCGAGGAAGCATTCTTTTGGAAGTGATGGCCAGAAGGAAAAAGGCGCCCGTGTTAAAAGGATCCTGGCGGCAGGCAAGGCTCTAGCAAATGTGGTCAAGGTTGATCAAAAAGCCATTTATTTTCATTCCCAGACGAAGAAATTTGTCATTGGTGTTGAGCCTCCAATGAATGGCGATTATGTGCCTACCTCCTTAGGCTTGCCTAATTCCAATGATAATCTGGAAGAAAATCAACTAAAGGACACTACGAATATGGGAGTTCCATTCCAAAAGTCAGAGTCGATCATTGATGGGGATGAGGAAGATGAAGTAATAGTTTTTAAGCCAATAGTGGCTGAGAAGAGACCTGATGTGGCAGGCACTACATGGGCTATTCCCCAGCCTTTGGAACCGTTTAAATCAGAAGAAGTATCTCTTGCTAACAACTTAAAGAGTTTGGGGTTTATGGGCAATGGACAAGTATTGAAATCTGAGCAAGTATCAAGTTCAGTTCCATTCCAACAGCCTGTCAATGGTAGTACTGGCAGTATGTTCTACAGTCATGCAAAGCATCCAGAAGCTTTGTTGCCGTTTAAGGTTGATACTATTGCATCGTCCGGACCAATTGCGGATGGTTTGACATTGAAGACGTCCTCAGCTTTCCCCGCTCCTGTCCGAAAAAATCCAGTTAGTCGACCTGTTAGGCACCTTGGTCCGCCACCTGGTTTTAGCCATGTTCCTGCTAAACAAGTCAATGAGTCTATTTATAATTCTGAGTCAATGGGTGAAAATCCACTGATGGATGATTACAGCTGGTTGGATGGGTATCAGGTGCCATCTTCAACTAAAGGAAACACATTCAGCAGTTCCATTAACTACTCATCTCATCCAAATGTGCTTCGTGTTCCTAATGGGAATGGGTTAAGTGGAACAGTGAACTTTCCCTTTCCTGGTAAACAGGGCCCGTCTATGCCATTCCAAGCAGAAAACCAGAATAGTCGGCAAGATTTCCGTATGCTTGAGGACCTTAAACTTCACCATGAAATGCAGCTGCAGCAGCAGCAACAGCAACAATTTGTAAATGGAAATCCGCACTTGAACCCACAACCTGAGCAATATCAAGGACAGTCTGTCTGGACAGGCCGCTATTTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

924

Amino Acids

102.58

Weight (kDa)

6.14

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 70 - 193 1.4e-17 Telomerase activating protein Est1
EST1_DNA_bind PF10373 206 - 539 1e-62 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2382
AatII GACGTC 1 cut(s) 2267
Acc36I ACCTGC 1 cut(s) 916
AccB1I GGYRCC 3 cut(s) 1679, 2319, 2446
AccB7I CCANNNNNTGG 4 cut(s) 138, 659, 1805, 2020
AccBSI CCGCTC 1 cut(s) 2282
AccI GTMKAC 1 cut(s) 2308
AccII CGCG 2 cut(s) 115, 603
AccIII TCCGGA 1 cut(s) 2231
AciI CCGC 9 cut(s) 241, 532, 805, 1703, 2243, 2280, 2330, 2705, 2758
AclWI GGATC 5 cut(s) 122, 251, 1310, 1690, 1703
AcoI YGGCCR 1 cut(s) 1663
AcsI RAATTY 1 cut(s) 1775
AcyI GRCGYC 3 cut(s) 94, 1680, 2264
AfaI GTAC 2 cut(s) 1230, 2155
AfiI CCNNNNNNNGG 9 cut(s) 138, 659, 748, 943, 1805, 2020, 2147, 2336, 2564
AgsI TTSAA 9 cut(s) 294, 313, 765, 987, 1462, 2101, 2260, 2457, 2713
AhdI GACNNNNNGTC 1 cut(s) 2744
AjnI CCWGG 8 cut(s) 137, 741, 963, 1032, 1339, 1698, 2335, 2563
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 267
Alw21I GWGCWC 3 cut(s) 55, 262, 1056
Alw26I GTCTC 4 cut(s) 1031, 1165, 1505, 1972
Alw44I GTGCAC 1 cut(s) 258
AlwI GGATC 5 cut(s) 122, 251, 1310, 1690, 1703
Ama87I CYCGRG 1 cut(s) 1636
Aor13HI TCCGGA 1 cut(s) 2231
AoxI GGCC 6 cut(s) 326, 690, 934, 1663, 2575, 2755
ApaI GGGCCC 1 cut(s) 2579
ApaLI GTGCAC 1 cut(s) 258
ApoI RAATTY 1 cut(s) 1775
AspLEI GCGC 2 cut(s) 115, 1682
AspS9I GGNCC 7 cut(s) 181, 317, 2234, 2327, 2575, 2576, 2638
AsuHPI GGTGA 4 cut(s) 521, 563, 2411, 2642
AvaI CYCGRG 1 cut(s) 1636
AvaII GGWCC 5 cut(s) 181, 317, 2234, 2327, 2638
AxyI CCTNAGG 1 cut(s) 1828
BaeGI GKGCMC 2 cut(s) 262, 2579
BalI TGGCCA 1 cut(s) 1665
BamHI GGATCC 1 cut(s) 1695
BanI GGYRCC 3 cut(s) 1679, 2319, 2446
BanII GRGCYC 1 cut(s) 2579
BbsI GAAGAC 2 cut(s) 1261, 2267
Bbv12I GWGCWC 3 cut(s) 55, 262, 1056
BbvCI CCTCAGC 1 cut(s) 2269
BceAI ACGGC 1 cut(s) 2188
BcgI CGANNNNNNTGC 4 cut(s) 239, 273, 593, 627
BciT130I CCWGG 8 cut(s) 139, 743, 965, 1034, 1341, 1700, 2337, 2565
BclI TGATCA 2 cut(s) 6, 1738
BcoDI GTCTC 4 cut(s) 1031, 1165, 1505, 1972
BfaI CTAG 4 cut(s) 176, 1029, 1385, 1718
BfmI CTRYAG 4 cut(s) 285, 1247, 2170, 2666
BfoI RGCGCY 1 cut(s) 1683
BfuAI ACCTGC 1 cut(s) 916
BlpI GCTNAGC 1 cut(s) 54
BmcAI AGTACT 1 cut(s) 2155
Bme1390I CCNGG 8 cut(s) 139, 743, 965, 1034, 1341, 1700, 2337, 2565
Bme18I GGWCC 5 cut(s) 181, 317, 2234, 2327, 2638
BmeRI GACNNNNNGTC 1 cut(s) 2744
BmeT110I CYCGRG 1 cut(s) 1636
BmgT120I GGNCC 7 cut(s) 181, 317, 2234, 2327, 2575, 2576, 2638
BmiI GGNNCC 7 cut(s) 318, 1681, 1697, 2025, 2321, 2448, 2577
BmrFI CCNGG 8 cut(s) 139, 743, 965, 1034, 1341, 1700, 2337, 2565
BmsI GCATC 4 cut(s) 650, 1390, 2194, 2234
BpiI GAAGAC 2 cut(s) 1261, 2267
BplI GAGNNNNNCTC 4 cut(s) 575, 607, 802, 834
BpmI CTGGAG 2 cut(s) 1055, 1116
Bpu10I CCTNAGC 2 cut(s) 2269, 2724
Bpu1102I GCTNAGC 1 cut(s) 54
BpuEI CTTGAG 2 cut(s) 604, 2654
Bsa29I ATCGAT 1 cut(s) 727
BsaAI YACGTR 1 cut(s) 152
BsaBI GATNNNNATC 1 cut(s) 2439
BsaHI GRCGYC 3 cut(s) 94, 1680, 2264
BsaI GGTCTC 2 cut(s) 1165, 1972
BsaJI CCNNGG 1 cut(s) 2323
BsaWI WCCGGW 1 cut(s) 2231
Bsc4I CCNNNNNNNGG 9 cut(s) 138, 659, 748, 943, 1805, 2020, 2147, 2336, 2564
Bse1I ACTGG 8 cut(s) 388, 693, 880, 957, 1146, 1326, 2161, 2300
Bse21I CCTNAGG 1 cut(s) 1828
Bse3DI GCAATG 2 cut(s) 838, 2092
Bse8I GATNNNNATC 1 cut(s) 2439
BseAI TCCGGA 1 cut(s) 2231
BseBI CCWGG 8 cut(s) 139, 743, 965, 1034, 1341, 1700, 2337, 2565
BseCI ATCGAT 1 cut(s) 727
BseDI CCNNGG 1 cut(s) 2323
BseGI GGATG 9 cut(s) 490, 953, 1141, 1936, 2185, 2251, 2422, 2440, 2500
BseJI GATNNNNATC 1 cut(s) 2439
BseLI CCNNNNNNNGG 9 cut(s) 138, 659, 748, 943, 1805, 2020, 2147, 2336, 2564
BseMI GCAATG 2 cut(s) 838, 2092
BseNI ACTGG 8 cut(s) 388, 693, 880, 957, 1146, 1326, 2161, 2300
BseRI GAGGAG 2 cut(s) 24, 1121
BseSI GKGCMC 2 cut(s) 262, 2579
BseYI CCCAGC 2 cut(s) 1309, 2013
BsgI GTGCAG 2 cut(s) 831, 849
Bsh1236I CGCG 2 cut(s) 115, 603
BshFI GGCC 6 cut(s) 328, 692, 936, 1665, 2577, 2757
BshNI GGYRCC 3 cut(s) 1679, 2319, 2446
BshVI ATCGAT 1 cut(s) 727
BsiHKAI GWGCWC 3 cut(s) 55, 262, 1056
BsiHKCI CYCGRG 1 cut(s) 1636
BsiSI CCGG 1 cut(s) 2232
BslFI GGGAC 4 cut(s) 602, 911, 1362, 1466
BslI CCNNNNNNNGG 9 cut(s) 138, 659, 748, 943, 1805, 2020, 2147, 2336, 2564
BsmAI GTCTC 4 cut(s) 1031, 1165, 1505, 1972
BsmFI GGGAC 4 cut(s) 602, 911, 1362, 1466
BsmI GAATGC 5 cut(s) 1011, 1258, 1344, 1447, 1645
BsnI GGCC 6 cut(s) 328, 692, 936, 1665, 2577, 2757
Bso31I GGTCTC 2 cut(s) 1165, 1972
BsoBI CYCGRG 1 cut(s) 1636
Bsp120I GGGCCC 1 cut(s) 2575
Bsp1286I GDGCHC 4 cut(s) 55, 262, 1056, 2579
Bsp13I TCCGGA 1 cut(s) 2231
Bsp1407I TGTACA 1 cut(s) 1228
Bsp143I GATC 9 cut(s) 6, 127, 243, 331, 411, 1315, 1695, 1738, 1917
Bsp1720I GCTNAGC 1 cut(s) 54
Bsp68I TCGCGA 1 cut(s) 603
BspACI CCGC 9 cut(s) 241, 532, 805, 1703, 2243, 2280, 2330, 2705, 2758
BspANI GGCC 6 cut(s) 328, 692, 936, 1665, 2577, 2757
BspDI ATCGAT 1 cut(s) 727
BspEI TCCGGA 1 cut(s) 2231
BspFNI CGCG 2 cut(s) 115, 603
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 7 cut(s) 318, 1681, 1697, 2025, 2321, 2448, 2577
BspMAI CTGCAG 2 cut(s) 1251, 2670
BspMI ACCTGC 1 cut(s) 916
BspPI GGATC 5 cut(s) 122, 251, 1310, 1690, 1703
BspT107I GGYRCC 3 cut(s) 1679, 2319, 2446
BspTNI GGTCTC 2 cut(s) 1165, 1972
BsrBI CCGCTC 1 cut(s) 2282
BsrDI GCAATG 2 cut(s) 838, 2092
BsrGI TGTACA 1 cut(s) 1228
BsrI ACTGG 8 cut(s) 388, 693, 880, 957, 1146, 1326, 2161, 2300
BssECI CCNNGG 1 cut(s) 2323
BssMI GATC 9 cut(s) 6, 127, 243, 331, 411, 1315, 1695, 1738, 1917
BssNI GRCGYC 3 cut(s) 94, 1680, 2264
BssT1I CCWWGG 1 cut(s) 2323
Bst2UI CCWGG 8 cut(s) 139, 743, 965, 1034, 1341, 1700, 2337, 2565
Bst6I CTCTTC 2 cut(s) 1098, 1970
BstACI GRCGYC 3 cut(s) 94, 1680, 2264
BstAPI GCANNNNNTGC 2 cut(s) 908, 1262
BstAUI TGTACA 1 cut(s) 1228
BstBAI YACGTR 1 cut(s) 152
BstEII GGTNACC 1 cut(s) 551
BstENI CCTNNNNNAGG 1 cut(s) 941
BstF5I GGATG 9 cut(s) 490, 953, 1141, 1936, 2185, 2251, 2422, 2440, 2500
BstFNI CGCG 2 cut(s) 115, 603
BstH2I RGCGCY 1 cut(s) 1683
BstHHI GCGC 2 cut(s) 115, 1682
BstKTI GATC 9 cut(s) 9, 130, 246, 334, 414, 1318, 1698, 1741, 1920
BstMAI GTCTC 4 cut(s) 1031, 1165, 1505, 1972
BstMBI GATC 9 cut(s) 6, 127, 243, 331, 411, 1315, 1695, 1738, 1917
BstNI CCWGG 8 cut(s) 139, 743, 965, 1034, 1341, 1700, 2337, 2565
BstNSI RCATGY 2 cut(s) 1289, 1374
BstPI GGTNACC 1 cut(s) 551
BstSCI CCNGG 8 cut(s) 137, 741, 963, 1032, 1339, 1698, 2335, 2563
BstSFI CTRYAG 4 cut(s) 285, 1247, 2170, 2666
BstSLI GKGCMC 2 cut(s) 262, 2579
BstUI CGCG 2 cut(s) 115, 603
BstV2I GAAGAC 2 cut(s) 1261, 2267
BstX2I RGATCY 2 cut(s) 127, 1695
BstYI RGATCY 2 cut(s) 127, 1695
Bsu15I ATCGAT 1 cut(s) 727
Bsu36I CCTNAGG 1 cut(s) 1828
BsuRI GGCC 6 cut(s) 328, 692, 936, 1665, 2577, 2757
BsuTUI ATCGAT 1 cut(s) 727
BtgZI GCGATG 1 cut(s) 720
BtsCI GGATG 9 cut(s) 490, 953, 1141, 1936, 2185, 2251, 2422, 2440, 2500
BtsI GCAGTG 2 cut(s) 753, 1075
BtsIMutI CAGTG 6 cut(s) 717, 753, 848, 1075, 2408, 2553
BtuMI TCGCGA 1 cut(s) 603
BveI ACCTGC 1 cut(s) 916
CciI TCATGA 1 cut(s) 394
CfoI GCGC 2 cut(s) 115, 1682
Cfr13I GGNCC 7 cut(s) 181, 317, 2234, 2327, 2575, 2576, 2638
ClaI ATCGAT 1 cut(s) 727
CseI GACGC 2 cut(s) 83, 908
CsiI ACCWGGT 1 cut(s) 2335
Csp6I GTAC 2 cut(s) 1229, 2154
CviQI GTAC 2 cut(s) 1229, 2154
DinI GGCGCC 1 cut(s) 1681
DpnI GATC 9 cut(s) 8, 129, 245, 333, 413, 1317, 1697, 1740, 1919
DpnII GATC 9 cut(s) 6, 127, 243, 331, 411, 1315, 1695, 1738, 1917
DraI TTTAAA 2 cut(s) 361, 2032
DriI GACNNNNNGTC 1 cut(s) 2744
EaeI YGGCCR 1 cut(s) 1663
Eam1104I CTCTTC 2 cut(s) 1098, 1970
Eam1105I GACNNNNNGTC 1 cut(s) 2744
EarI CTCTTC 2 cut(s) 1098, 1970
EciI GGCGGA 1 cut(s) 2319
Eco130I CCWWGG 1 cut(s) 2323
Eco147I AGGCCT 1 cut(s) 936
Eco24I GRGCYC 1 cut(s) 2579
Eco31I GGTCTC 2 cut(s) 1165, 1972
Eco32I GATATC 1 cut(s) 522
Eco47I GGWCC 5 cut(s) 181, 317, 2234, 2327, 2638
Eco81I CCTNAGG 1 cut(s) 1828
Eco88I CYCGRG 1 cut(s) 1636
Eco91I GGTNACC 1 cut(s) 551
EcoNI CCTNNNNNAGG 1 cut(s) 941
EcoO109I RGGNCCY 3 cut(s) 317, 2575, 2638
EcoO65I GGTNACC 1 cut(s) 551
EcoRII CCWGG 8 cut(s) 137, 741, 963, 1032, 1339, 1698, 2335, 2563
EcoRV GATATC 1 cut(s) 522
EcoT14I CCWWGG 1 cut(s) 2323
EcoT22I ATGCAT 2 cut(s) 190, 1258
EcoT38I GRGCYC 1 cut(s) 2579
EgeI GGCGCC 1 cut(s) 1681
EheI GGCGCC 1 cut(s) 1681
ErhI CCWWGG 1 cut(s) 2323
FalI AAGNNNNNCTT 4 cut(s) 921, 953, 2034, 2066
FaqI GGGAC 4 cut(s) 602, 911, 1362, 1466
FauI CCCGC 1 cut(s) 2287
FauNDI CATATG 1 cut(s) 1216
FbaI TGATCA 2 cut(s) 6, 1738
FblI GTMKAC 1 cut(s) 2308
FokI GGATG 9 cut(s) 497, 940, 1148, 1943, 2172, 2258, 2429, 2447, 2487
FriOI GRGCYC 1 cut(s) 2579
FspBI CTAG 4 cut(s) 176, 1029, 1385, 1718
GlaI GCGC 2 cut(s) 114, 1681
GsaI CCCAGC 2 cut(s) 1313, 2017
GsuI CTGGAG 2 cut(s) 1055, 1116
HaeII RGCGCY 1 cut(s) 1683
HaeIII GGCC 6 cut(s) 328, 692, 936, 1665, 2577, 2757
HapII CCGG 1 cut(s) 2232
HgaI GACGC 2 cut(s) 83, 908
HhaI GCGC 2 cut(s) 115, 1682
Hin1I GRCGYC 3 cut(s) 94, 1680, 2264
Hin6I GCGC 2 cut(s) 113, 1680
HinP1I GCGC 2 cut(s) 113, 1680
HincII GTYRAC 1 cut(s) 2309
HindII GTYRAC 1 cut(s) 2309
HindIII AAGCTT 2 cut(s) 978, 2193
HinfI GANTC 7 cut(s) 455, 590, 664, 1558, 1913, 2372, 2390
HpaII CCGG 1 cut(s) 2232
HphI GGTGA 4 cut(s) 521, 563, 2411, 2642
Hpy166II GTNNAC 4 cut(s) 260, 2309, 2551, 2570
Hpy8I GTNNAC 4 cut(s) 260, 2309, 2551, 2570
Hpy99I CGWCG 2 cut(s) 99, 251
HpyAV CCTTC 5 cut(s) 93, 307, 1199, 1538, 1663
HpyCH4IV ACGT 4 cut(s) 151, 868, 1234, 2264
HpySE526I ACGT 4 cut(s) 151, 868, 1234, 2264
Hsp92I GRCGYC 3 cut(s) 94, 1680, 2264
HspAI GCGC 2 cut(s) 113, 1680
KasI GGCGCC 1 cut(s) 1679
Kpn2I TCCGGA 1 cut(s) 2231
Ksp22I TGATCA 2 cut(s) 6, 1738
Kzo9I GATC 9 cut(s) 6, 127, 243, 331, 411, 1315, 1695, 1738, 1917
LmnI GCTCC 5 cut(s) 37, 46, 1097, 1108, 2287
LweI GCATC 4 cut(s) 650, 1390, 2194, 2234
MabI ACCWGGT 1 cut(s) 2335
MaeI CTAG 4 cut(s) 176, 1029, 1385, 1718
MaeII ACGT 4 cut(s) 151, 868, 1234, 2264
MaeIII GTNAC 9 cut(s) 228, 527, 551, 623, 715, 812, 959, 1328, 1335
MalI GATC 9 cut(s) 8, 129, 245, 333, 413, 1317, 1697, 1740, 1919
MbiI CCGCTC 1 cut(s) 2282
MboI GATC 9 cut(s) 6, 127, 243, 331, 411, 1315, 1695, 1738, 1917
MfeI CAATTG 1 cut(s) 2238
MflI RGATCY 2 cut(s) 127, 1695
MhlI GDGCHC 4 cut(s) 55, 262, 1056, 2579
MlsI TGGCCA 1 cut(s) 1665
MluNI TGGCCA 1 cut(s) 1665
Mly113I GGCGCC 1 cut(s) 1680
MlyI GAGTC 4 cut(s) 584, 1922, 2381, 2399
MmeI TCCRAC 4 cut(s) 63, 1343, 2158, 2412
Mox20I TGGCCA 1 cut(s) 1665
Mph1103I ATGCAT 2 cut(s) 190, 1258
MroI TCCGGA 1 cut(s) 2231
MscI TGGCCA 1 cut(s) 1665
MslI CAYNNNNRTG 2 cut(s) 267, 2505
Msp20I TGGCCA 1 cut(s) 1665
MspA1I CMGCKG 4 cut(s) 534, 1391, 2427, 2665
MspI CCGG 1 cut(s) 2232
MspR9I CCNGG 8 cut(s) 139, 743, 965, 1034, 1341, 1700, 2337, 2565
MunI CAATTG 1 cut(s) 2238
Mva1269I GAATGC 5 cut(s) 1011, 1258, 1344, 1447, 1645
MvaI CCWGG 8 cut(s) 139, 743, 965, 1034, 1341, 1700, 2337, 2565
MvnI CGCG 2 cut(s) 115, 603
NarI GGCGCC 1 cut(s) 1680
NdeI CATATG 1 cut(s) 1216
NdeII GATC 9 cut(s) 6, 127, 243, 331, 411, 1315, 1695, 1738, 1917
NlaIV GGNNCC 7 cut(s) 318, 1681, 1697, 2025, 2321, 2448, 2577
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 3 cut(s) 527, 551, 715
NruI TCGCGA 1 cut(s) 603
NsiI ATGCAT 2 cut(s) 190, 1258
NspI RCATGY 2 cut(s) 1289, 1374
OliI CACNNNNGTG 1 cut(s) 267
PaeI GCATGC 1 cut(s) 1374
PaeR7I CTCGAG 1 cut(s) 1636
PagI TCATGA 1 cut(s) 394
PceI AGGCCT 1 cut(s) 936
PcsI WCGNNNNNNNCGW 3 cut(s) 331, 733, 997
PctI GAATGC 5 cut(s) 1011, 1258, 1344, 1447, 1645
PfeI GAWTC 3 cut(s) 455, 664, 1558
PflMI CCANNNNNTGG 4 cut(s) 138, 659, 1805, 2020
PleI GAGTC 4 cut(s) 584, 1921, 2380, 2398
PluTI GGCGCC 1 cut(s) 1683
PpsI GAGTC 4 cut(s) 584, 1921, 2380, 2398
Ppu21I YACGTR 1 cut(s) 152
PpuMI RGGWCCY 2 cut(s) 317, 2638
PsiI TTATAA 1 cut(s) 2382
Psp5II RGGWCCY 2 cut(s) 317, 2638
Psp6I CCWGG 8 cut(s) 137, 741, 963, 1032, 1339, 1698, 2335, 2563
PspEI GGTNACC 1 cut(s) 551
PspFI CCCAGC 2 cut(s) 1309, 2013
PspGI CCWGG 8 cut(s) 137, 741, 963, 1032, 1339, 1698, 2335, 2563
PspN4I GGNNCC 7 cut(s) 318, 1681, 1697, 2025, 2321, 2448, 2577
PspOMI GGGCCC 1 cut(s) 2575
PspPI GGNCC 7 cut(s) 181, 317, 2234, 2327, 2575, 2576, 2638
PspPPI RGGWCCY 2 cut(s) 317, 2638
PstI CTGCAG 2 cut(s) 1251, 2670
PsuI RGATCY 2 cut(s) 127, 1695
PvuII CAGCTG 3 cut(s) 1391, 2427, 2665
RruI TCGCGA 1 cut(s) 603
RsaI GTAC 2 cut(s) 1230, 2155
RsaNI GTAC 2 cut(s) 1229, 2154
RseI CAYNNNNRTG 2 cut(s) 267, 2505
SalI GTCGAC 1 cut(s) 2307
Sau3AI GATC 9 cut(s) 6, 127, 243, 331, 411, 1315, 1695, 1738, 1917
Sau96I GGNCC 7 cut(s) 181, 317, 2234, 2327, 2575, 2576, 2638
ScaI AGTACT 1 cut(s) 2155
SchI GAGTC 4 cut(s) 584, 1922, 2381, 2399
ScrFI CCNGG 8 cut(s) 139, 743, 965, 1034, 1341, 1700, 2337, 2565
SduI GDGCHC 4 cut(s) 55, 262, 1056, 2579
SexAI ACCWGGT 1 cut(s) 2335
SfaNI GCATC 4 cut(s) 650, 1390, 2194, 2234
SfcI CTRYAG 4 cut(s) 285, 1247, 2170, 2666
SfoI GGCGCC 1 cut(s) 1681
Sfr274I CTCGAG 1 cut(s) 1636
SinI GGWCC 5 cut(s) 181, 317, 2234, 2327, 2638
SlaI CTCGAG 1 cut(s) 1636
SmiMI CAYNNNNRTG 2 cut(s) 267, 2505
SmlI CTYRAG 3 cut(s) 619, 1636, 2633
SmoI CTYRAG 3 cut(s) 619, 1636, 2633
SphI GCATGC 1 cut(s) 1374
SseBI AGGCCT 1 cut(s) 936
SsiI CCGC 9 cut(s) 241, 532, 805, 1703, 2243, 2280, 2330, 2705, 2758
SspDI GGCGCC 1 cut(s) 1679
SspI AATATT 2 cut(s) 1173, 1626
SspMI CTAG 4 cut(s) 176, 1029, 1385, 1718
StuI AGGCCT 1 cut(s) 936
StyD4I CCNGG 8 cut(s) 137, 741, 963, 1032, 1339, 1698, 2335, 2563
StyI CCWWGG 1 cut(s) 2323
TaiI ACGT 4 cut(s) 154, 871, 1237, 2267
TaqI TCGA 9 cut(s) 97, 246, 466, 727, 872, 1272, 1637, 1916, 2308
TatI WGTACW 2 cut(s) 1228, 2153
TauI GCSGC 2 cut(s) 1706, 2760
TfiI GAWTC 3 cut(s) 455, 664, 1558
TscAI CASTG 6 cut(s) 724, 753, 855, 1075, 2415, 2553
TseFI GTSAC 3 cut(s) 527, 551, 715
Tsp45I GTSAC 3 cut(s) 527, 551, 715
TspDTI ATGAA 9 cut(s) 177, 521, 1172, 1422, 1554, 1748, 1817, 1956, 2670
TspGWI ACGGA 1 cut(s) 2615
TspRI CASTG 6 cut(s) 724, 753, 855, 1075, 2415, 2553
Van91I CCANNNNNTGG 4 cut(s) 138, 659, 1805, 2020
VneI GTGCAC 1 cut(s) 258
VpaK11BI GGWCC 5 cut(s) 181, 317, 2234, 2327, 2638
XagI CCTNNNNNAGG 1 cut(s) 941
XapI RAATTY 1 cut(s) 1775
XbaI TCTAGA 1 cut(s) 175
XceI RCATGY 2 cut(s) 1289, 1374
XcmI CCANNNNNNNNNTGG 1 cut(s) 1852
XhoI CTCGAG 1 cut(s) 1636
XmiI GTMKAC 1 cut(s) 2308
XspI CTAG 4 cut(s) 176, 1029, 1385, 1718
ZraI GACGTC 1 cut(s) 2265
ZrmI AGTACT 1 cut(s) 2155
Zsp2I ATGCAT 2 cut(s) 190, 1258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.