Prupe.4G265800_v2.0.a1

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0008095
Physical Location & Seq
Forward (+)
19830193 .. 19837697
7505 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G265800.1

Sequence Viewer

Length: 3042 bp
ATGATTACACGTATGGATAAAATGTCAGCTCCTTCATCAAGGGAGCGTGCCCAACGCCTCTATGACAAGGTTATTGAATTGGAGAATAGGCGTAGGAGGTCTGCTCAAGCTCGAATTCCCTCAGATCCCAATGCCTGGCAACAGATACGGGAGAATTATGAAGCAATAATTCTCGAGGATCATGCTTTTTCTGAGCAGCACAATATTGAGTATGCTTTGTGGCAGCTGCATTACAAGCGTATTGAGGAATTGAGAGCACACTTTAGTGCTGCTACGGCTTCTGCAGGTTCAAACTCATCTCAGGCTGTGAAGGGCCCTGCTCGGCCTGATCGGATTACAAAAATAAGATTGCAGTTTAAAACATTCCTTTCAGAGGCAACTGGATTTTATCATGATTTGATAGTGAAGATCAGAGCAAAGTATGGGCTCCCTCTCGGGTATTTCTCTGAGGATTCTGAAAATAAAATTGTTATGGACAAGGATGGAAAGAAATCTACTGAAATGAAGAAAGGCCTGATATCGTGCCACCGATGTTTGATCTACTTGGGTGATCTTGCACGCTACAAAGGATTGTATGGGGAGGGGGACTCCAAAACTCGTGAGTATGCTGCAGCCTCAAGTTACTACTTGCAAGCTGCATCTCTCTGGCCATCAAGTGGGAATCCCCATCATCAGCTTGCTATTTTGGCATCCTATTCGGGGGATGAGTTGGTGGCTGTTTATCGATATTTTCGTAGCCTGGCGGTGGATAGTCCCTTTTCAACTGCAAGGGATAACTTGATTGTTGCATTTGAGAAGAATCGTCAGAGTTATTCTCAACTGCCTGGGAATACTAATGCTTCTGCAGTCAAAGAGTTGCCTGCACGATTGACAAGCAAAGGTAGAGGGAAAGCGGAAGCAATACCTGCATCTAAAGATAACAATACAGAAGTGAGCCTTGTTAAGGAAAAAGCATCCAGTACTCAGGAAACTTATAAGGCTTTTTGCATACGGTTTGTCCGTCTAAATGGGATTCTTTTCACACGTACTAGCCTGGAGACATTTGTAGAAGTTCTCTCTGTGGTAAGCAGTGGTTTATGTGAGCTCCTGTCTTCTGGAGCAGAGGAGTTGCAGAATTTCGGTGCAGATTCTGTTGAGAATGGACTTTTCATTGTTAGACTTGTCTCCATATTGATATTCACAGTTCACAATGTGAAGAAGGAAAGTGAAGGTCAGACATATGCTGAAATTGTACAACGTGCTGTCGTACTGCAGAATGCATTTACTGCTGTCTTTGAGTTGATGGGGCACATCTTAGAGAGATGCGTCCAGTTGTGTGATCCTTCTTCAAGTTTCCTGTTACCGGGCATTCTGGTGTTTGTAGAATGGTTAGCATGTTGCCCGGATGTTGCAGCTGGAAGCGATGCAGATGAAAAACAGACTAAAGTTAGATCAAAGTTCTGGATGGTTTGCATCTCCTTCCTGAATAGTATCTCGTCAACAGGGCCGGTGTCCATAGATGATGATGAAGATGAGACTTGCTTTAATAATATGAGCAGATATGAAGAAGGGGAAACTGAGAACCGTCTTGCTTTGTGGGAGGATTTTGAGTTGAGAGGATTCATTCCACTTCTTCCAGCGCAAACTATATTGGACTTCTCAAGGAAGCATTCTTTTGGAAGTGACGGCCACAAGGAAAAAGGGGCCCGTGTTAAACGGATTGTGGCGGCAGGCAAGGCTCTTGCAAATGTGATTAAGGTTGACCAGAAAGCAGTATATTTTGATTCGAAGGCAAAGAAATTTGTCATTGGTTTTGAGCCTCCAGTGCAAAATGATTTTGTTCCTACCTCCTATATGGGCATGGCTACTGAAAATGATAATCTGCAAGAAAATCAAGCAGAGAATACAATGAAGTTGGGAGTTGCATATCCAAAGCCTGAGTTAACCATGGAAGGGGATGAGGAAGATGAAGTAATAGTTTTTAAGCCAATAGTGGCTGAGAAGCGGCCTGATGTGGTAAACACTACTTGGGCAGCCTATGAGGGCCTGGTGCCTGGTAAAAATGCTTCTCCAGGTGATCTTAAAGTTAATGGCACTTATGTCACTGCCCCTTTTGATAATCTTCGCCATCAAACCGCTTTTAGTGCTGGTTCACAAATACCTGTATCTCTTGGTAATGGTATACCCCAACATTTGCAATCAATTCAGTCACATGCTTCCAAGCTGTCAATGGAAGCTGGTTTTGGTGCTAGTTCACAACTTCCTGTATCAGTTGCTAATAGCATCCCCCAGAATTTGCAACCAACTCAATCACATGCTTTGAAGTTGTCAACAGAGGAAGAAATGTCTCTTGCTCACGGCTTAAAGAGTATGGGATTTATGGGGAATGGGTATGTACTTGCATCTGAGCCTGTGGCAGTTTCAGTTCCATTCCAGCAGCCAGTGAATGGTAGTACTAGTGGTATGGTCTACAGCCACACAAAAGCTCCAGAAGCTATGTTGCCGTTTAAGGTTGATGCTATGTCATCTTCTGGAGCAATTGCTGACGGTTTGACTGTAAAGACATCATCTAACCTGCCCACAGGTATCCGAAAGAATCCAGTAAGTCGACCTGTTAGGCATCTTGGTCCACCACCTGGTTTTAGCCCTGTTCCTCCTAAAAATGTGAATGAATCGATTTATGGCTCCGACTCAATGAGTGAGAATCTACTGATGGACGATTACAGCTGGTTGGATGGATATCAGATGCCATCTTCAACAAAAGGAAATGGGCTCAATAGCTCCATTAATATCTCATCTCATTCAAATCCCAATCGCTTCATTAACAGCAATGGCTTGAATGGGCCAGTGAACTTTCCCTTTCCTGGTAAACTGGGTCCACCTATGCAACTCCAAGGCGAAAAACAGAAAAGCTGGCAAGATTTTCAGATGCTCGATGAGCTCAAACTACACCATGAAATGCAGCTGCAGCAACAACAACTTGTAAATGGAAATCAGCACTTGACTCCTCAGCCTGAGCAATATCAAGGACAGTCTGTCTGGACAGGCCGTTATTTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

1014

Amino Acids

111.97

Weight (kDa)

6.26

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 975
Acc36I ACCTGC 3 cut(s) 275, 913, 2562
AccB1I GGYRCC 1 cut(s) 2029
AccB7I CCANNNNNTGG 4 cut(s) 135, 656, 2426, 2615
AccI GTMKAC 3 cut(s) 2161, 2448, 2587
AciI CCGC 5 cut(s) 743, 893, 1706, 1984, 2115
AclWI GGATC 3 cut(s) 119, 186, 1313
AcoI YGGCCR 2 cut(s) 647, 1666
AcsI RAATTY 4 cut(s) 114, 1114, 1778, 2272
AdeI CACNNNGTG 1 cut(s) 1192
AfaI GTAC 6 cut(s) 961, 1027, 1233, 1248, 2376, 2434
AflIII ACRYGT 2 cut(s) 8, 1022
AgsI TTSAA 8 cut(s) 77, 291, 762, 1329, 2302, 2736, 2784, 2818
AhdI GACNNNNNGTC 1 cut(s) 3014
AhlI ACTAGT 1 cut(s) 2435
AjnI CCWGG 9 cut(s) 134, 738, 823, 1032, 2025, 2032, 2050, 2614, 2842
AjuI GAANNNNNNNTTGG 2 cut(s) 2205, 2237
AleI CACNNNNGTG 1 cut(s) 264
Alw21I GWGCWC 3 cut(s) 259, 1086, 2922
Alw26I GTCTC 4 cut(s) 1031, 1168, 1508, 2331
AlwI GGATC 3 cut(s) 119, 186, 1313
AlwNI CAGNNNCTG 1 cut(s) 1130
Ama87I CYCGRG 2 cut(s) 173, 434
ApaI GGGCCC 2 cut(s) 317, 1687
ApoI RAATTY 4 cut(s) 114, 1114, 1778, 2272
AseI ATTAAT 1 cut(s) 2766
AspLEI GCGC 1 cut(s) 1621
AspS9I GGNCC 9 cut(s) 313, 314, 1484, 1683, 1684, 2023, 2606, 2822, 2855
AsuC2I CCSGG 2 cut(s) 1344, 1382
AsuHPI GGTGA 2 cut(s) 560, 2066
AsuII TTCGAA 1 cut(s) 1766
AvaI CYCGRG 2 cut(s) 173, 434
AvaII GGWCC 2 cut(s) 2606, 2855
BaeGI GKGCMC 4 cut(s) 52, 317, 1290, 1687
BalI TGGCCA 1 cut(s) 649
BanI GGYRCC 1 cut(s) 2029
BanII GRGCYC 6 cut(s) 317, 429, 1086, 1687, 2754, 2922
BarI GAAGNNNNNNTAC 2 cut(s) 2029, 2061
BauI CACGAG 1 cut(s) 597
BbsI GAAGAC 1 cut(s) 1083
Bbv12I GWGCWC 3 cut(s) 259, 1086, 2922
BbvCI CCTCAGC 1 cut(s) 2988
BccI CCATC 9 cut(s) 476, 658, 675, 1276, 1438, 2115, 2686, 2708, 2737
BceAI ACGGC 5 cut(s) 291, 1681, 2353, 2467, 3012
BcgI CGANNNNNNTGC 4 cut(s) 164, 198, 678, 712
BciT130I CCWGG 9 cut(s) 136, 740, 825, 1034, 2027, 2034, 2052, 2616, 2844
BciVI GTATCC 1 cut(s) 2576
BcnI CCSGG 2 cut(s) 1344, 1382
BcoDI GTCTC 4 cut(s) 1031, 1168, 1508, 2331
BcuI ACTAGT 1 cut(s) 2435
BfaI CTAG 3 cut(s) 1029, 2229, 2436
BfmI CTRYAG 6 cut(s) 282, 609, 843, 1250, 2449, 2945
BfuAI ACCTGC 3 cut(s) 275, 913, 2562
BfuI GTATCC 1 cut(s) 2576
BmcAI AGTACT 2 cut(s) 961, 2434
Bme18I GGWCC 2 cut(s) 2606, 2855
BmeRI GACNNNNNGTC 1 cut(s) 3014
BmeT110I CYCGRG 2 cut(s) 173, 434
BmgT120I GGNCC 9 cut(s) 313, 314, 1484, 1683, 1684, 2023, 2606, 2822, 2855
BmiI GGNNCC 7 cut(s) 315, 428, 1684, 1685, 2031, 2665, 2856
BmrI ACTGGG 1 cut(s) 2861
BmuI ACTGGG 1 cut(s) 2861
BpiI GAAGAC 1 cut(s) 1083
BplI GAGNNNNNCTC 6 cut(s) 88, 120, 572, 604, 799, 831
BpmI CTGGAG 6 cut(s) 1055, 1116, 1785, 2034, 2451, 2532
Bpu10I CCTNAGC 2 cut(s) 2988, 2994
Bpu14I TTCGAA 1 cut(s) 1766
BpuEI CTTGAG 3 cut(s) 90, 601, 1624
BpuMI CCSGG 2 cut(s) 1344, 1382
Bsa29I ATCGAT 2 cut(s) 724, 2654
BsaAI YACGTR 2 cut(s) 11, 1025
BsaBI GATNNNNATC 1 cut(s) 2718
BsaJI CCNNGG 3 cut(s) 824, 1926, 2872
BsaXI ACNNNNNCTCC 2 cut(s) 2449, 2479
Bse118I RCCGGY 1 cut(s) 1486
Bse1I ACTGG 8 cut(s) 385, 957, 1309, 1802, 2420, 2579, 2825, 2856
Bse3DI GCAATG 1 cut(s) 2815
Bse8I GATNNNNATC 1 cut(s) 2718
BseBI CCWGG 9 cut(s) 136, 740, 825, 1034, 2027, 2034, 2052, 2616, 2844
BseCI ATCGAT 2 cut(s) 724, 2654
BseDI CCNNGG 3 cut(s) 824, 1926, 2872
BseGI GGATG 9 cut(s) 487, 689, 709, 953, 1390, 1449, 1942, 2262, 2719
BseJI GATNNNNATC 1 cut(s) 2718
BseMI GCAATG 1 cut(s) 2815
BseNI ACTGG 8 cut(s) 385, 957, 1309, 1802, 2420, 2579, 2825, 2856
BseRI GAGGAG 2 cut(s) 1118, 2976
BseSI GKGCMC 4 cut(s) 52, 317, 1290, 1687
BsgI GTGCAG 2 cut(s) 846, 1143
BshNI GGYRCC 1 cut(s) 2029
BshVI ATCGAT 2 cut(s) 724, 2654
BsiHKAI GWGCWC 3 cut(s) 259, 1086, 2922
BsiHKCI CYCGRG 2 cut(s) 173, 434
BsiSI CCGG 3 cut(s) 1343, 1382, 1487
BslFI GGGAC 2 cut(s) 599, 738
BsmAI GTCTC 4 cut(s) 1031, 1168, 1508, 2331
BsmFI GGGAC 2 cut(s) 599, 738
BsmI GAATGC 3 cut(s) 1261, 1347, 1648
BsoBI CYCGRG 2 cut(s) 173, 434
Bsp119I TTCGAA 1 cut(s) 1766
Bsp120I GGGCCC 2 cut(s) 313, 1683
Bsp1286I GDGCHC 9 cut(s) 52, 259, 317, 429, 1086, 1290, 1687, 2754, 2922
Bsp1407I TGTACA 1 cut(s) 1231
Bsp143I GATC 9 cut(s) 124, 178, 328, 408, 537, 550, 1318, 1430, 2056
Bsp19I CCATGG 1 cut(s) 1926
BspACI CCGC 5 cut(s) 743, 893, 1706, 1984, 2115
BspDI ATCGAT 2 cut(s) 724, 2654
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 7 cut(s) 315, 428, 1684, 1685, 2031, 2665, 2856
BspMAI CTGCAG 5 cut(s) 286, 613, 847, 1254, 2949
BspMI ACCTGC 3 cut(s) 275, 913, 2562
BspPI GGATC 3 cut(s) 119, 186, 1313
BspT104I TTCGAA 1 cut(s) 1766
BspT107I GGYRCC 1 cut(s) 2029
BsrDI GCAATG 1 cut(s) 2815
BsrFI RCCGGY 1 cut(s) 1486
BsrGI TGTACA 1 cut(s) 1231
BsrI ACTGG 8 cut(s) 385, 957, 1309, 1802, 2420, 2579, 2825, 2856
BssAI RCCGGY 1 cut(s) 1486
BssECI CCNNGG 3 cut(s) 824, 1926, 2872
BssMI GATC 9 cut(s) 124, 178, 328, 408, 537, 550, 1318, 1430, 2056
BssNAI GTATAC 1 cut(s) 2162
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 2 cut(s) 1926, 2872
Bst1107I GTATAC 1 cut(s) 2162
Bst2BI CACGAG 1 cut(s) 597
Bst2UI CCWGG 9 cut(s) 136, 740, 825, 1034, 2027, 2034, 2052, 2616, 2844
Bst4CI ACNGT 6 cut(s) 993, 1183, 1565, 2528, 2536, 3012
BstAPI GCANNNNNTGC 2 cut(s) 905, 1265
BstAUI TGTACA 1 cut(s) 1231
BstBAI YACGTR 2 cut(s) 11, 1025
BstBI TTCGAA 1 cut(s) 1766
BstC8I GCNNGC 7 cut(s) 48, 559, 633, 678, 861, 1711, 2894
BstDSI CCRYGG 1 cut(s) 1926
BstENI CCTNNNNNAGG 2 cut(s) 371, 941
BstF5I GGATG 9 cut(s) 487, 689, 709, 953, 1390, 1449, 1942, 2262, 2719
BstHHI GCGC 1 cut(s) 1621
BstKTI GATC 9 cut(s) 127, 181, 331, 411, 540, 553, 1321, 1433, 2059
BstMAI GTCTC 4 cut(s) 1031, 1168, 1508, 2331
BstMBI GATC 9 cut(s) 124, 178, 328, 408, 537, 550, 1318, 1430, 2056
BstNI CCWGG 9 cut(s) 136, 740, 825, 1034, 2027, 2034, 2052, 2616, 2844
BstNSI RCATGY 3 cut(s) 1377, 2195, 2297
BstSFI CTRYAG 6 cut(s) 282, 609, 843, 1250, 2449, 2945
BstSLI GKGCMC 4 cut(s) 52, 317, 1290, 1687
BstV2I GAAGAC 1 cut(s) 1083
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BstZ17I GTATAC 1 cut(s) 2162
Bsu15I ATCGAT 2 cut(s) 724, 2654
BsuI GTATCC 1 cut(s) 2576
BsuTUI ATCGAT 2 cut(s) 724, 2654
BtgI CCRYGG 1 cut(s) 1926
BtgZI GCGATG 1 cut(s) 1416
BtsCI GGATG 9 cut(s) 487, 689, 709, 953, 1390, 1449, 1942, 2262, 2719
BtsI GCAGTG 2 cut(s) 1075, 2082
BtsIMutI CAGTG 5 cut(s) 1075, 1809, 2082, 2427, 2832
BveI ACCTGC 3 cut(s) 275, 913, 2562
Cac8I GCNNGC 7 cut(s) 48, 559, 633, 678, 861, 1711, 2894
CaiI CAGNNNCTG 1 cut(s) 1130
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 1621
Cfr10I RCCGGY 1 cut(s) 1486
Cfr13I GGNCC 9 cut(s) 313, 314, 1484, 1683, 1684, 2023, 2606, 2822, 2855
ClaI ATCGAT 2 cut(s) 724, 2654
CseI GACGC 1 cut(s) 1294
CsiI ACCWGGT 1 cut(s) 2614
Csp6I GTAC 6 cut(s) 960, 1026, 1232, 1247, 2375, 2433
CviAII CATG 8 cut(s) 182, 392, 1374, 1840, 1927, 2192, 2294, 2933
CviQI GTAC 6 cut(s) 960, 1026, 1232, 1247, 2375, 2433
DpnI GATC 9 cut(s) 126, 180, 330, 410, 539, 552, 1320, 1432, 2058
DpnII GATC 9 cut(s) 124, 178, 328, 408, 537, 550, 1318, 1430, 2056
DraI TTTAAA 1 cut(s) 358
DraIII CACNNNGTG 1 cut(s) 1192
DriI GACNNNNNGTC 1 cut(s) 3014
EaeI YGGCCR 2 cut(s) 647, 1666
Eam1105I GACNNNNNGTC 1 cut(s) 3014
Ecl136II GAGCTC 2 cut(s) 1084, 2920
Eco130I CCWWGG 2 cut(s) 1926, 2872
Eco147I AGGCCT 1 cut(s) 513
Eco24I GRGCYC 6 cut(s) 317, 429, 1086, 1687, 2754, 2922
Eco32I GATATC 2 cut(s) 519, 2720
Eco47I GGWCC 2 cut(s) 2606, 2855
Eco53kI GAGCTC 2 cut(s) 1084, 2920
Eco88I CYCGRG 2 cut(s) 173, 434
EcoICRI GAGCTC 2 cut(s) 1084, 2920
EcoNI CCTNNNNNAGG 2 cut(s) 371, 941
EcoO109I RGGNCCY 4 cut(s) 313, 314, 1683, 2023
EcoRI GAATTC 1 cut(s) 114
EcoRII CCWGG 9 cut(s) 134, 738, 823, 1032, 2025, 2032, 2050, 2614, 2842
EcoRV GATATC 2 cut(s) 519, 2720
EcoT14I CCWWGG 2 cut(s) 1926, 2872
EcoT22I ATGCAT 1 cut(s) 1261
EcoT38I GRGCYC 6 cut(s) 317, 429, 1086, 1687, 2754, 2922
ErhI CCWWGG 2 cut(s) 1926, 2872
FaeI CATG 8 cut(s) 185, 395, 1377, 1843, 1930, 2195, 2297, 2936
FalI AAGNNNNNCTT 2 cut(s) 921, 953
FaqI GGGAC 2 cut(s) 599, 738
FatI CATG 8 cut(s) 181, 391, 1373, 1839, 1926, 2191, 2293, 2932
FauNDI CATATG 1 cut(s) 1219
FblI GTMKAC 3 cut(s) 2161, 2448, 2587
FokI GGATG 9 cut(s) 494, 676, 716, 940, 1397, 1456, 1949, 2249, 2726
FriOI GRGCYC 6 cut(s) 317, 429, 1086, 1687, 2754, 2922
FspBI CTAG 3 cut(s) 1029, 2229, 2436
GlaI GCGC 1 cut(s) 1620
GsuI CTGGAG 6 cut(s) 1055, 1116, 1785, 2034, 2451, 2532
HapII CCGG 3 cut(s) 1343, 1382, 1487
HgaI GACGC 1 cut(s) 1294
HhaI GCGC 1 cut(s) 1621
Hin1II CATG 8 cut(s) 185, 395, 1377, 1843, 1930, 2195, 2297, 2936
Hin6I GCGC 1 cut(s) 1619
HinP1I GCGC 1 cut(s) 1619
HincII GTYRAC 5 cut(s) 1479, 1741, 1923, 2310, 2588
HindII GTYRAC 5 cut(s) 1479, 1741, 1923, 2310, 2588
HpaI GTTAAC 1 cut(s) 1923
HpaII CCGG 3 cut(s) 1343, 1382, 1487
HphI GGTGA 2 cut(s) 560, 2066
HpyAV CCTTC 9 cut(s) 42, 304, 1192, 1202, 1332, 1468, 1541, 1762, 1925
HpyCH4III ACNGT 6 cut(s) 993, 1183, 1565, 2528, 2536, 3012
HpyCH4IV ACGT 3 cut(s) 10, 1024, 1237
HpySE526I ACGT 3 cut(s) 10, 1024, 1237
Hsp92II CATG 8 cut(s) 185, 395, 1377, 1843, 1930, 2195, 2297, 2936
HspAI GCGC 1 cut(s) 1619
KspAI GTTAAC 1 cut(s) 1923
Kzo9I GATC 9 cut(s) 124, 178, 328, 408, 537, 550, 1318, 1430, 2056
LmnI GCTCC 9 cut(s) 34, 43, 432, 1089, 1097, 2470, 2513, 2669, 2765
MabI ACCWGGT 1 cut(s) 2614
MaeI CTAG 3 cut(s) 1029, 2229, 2436
MaeII ACGT 3 cut(s) 10, 1024, 1237
MaeIII GTNAC 5 cut(s) 620, 1338, 1661, 2080, 2187
MalI GATC 9 cut(s) 126, 180, 330, 410, 539, 552, 1320, 1432, 2058
MboI GATC 9 cut(s) 124, 178, 328, 408, 537, 550, 1318, 1430, 2056
MfeI CAATTG 1 cut(s) 2517
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 9 cut(s) 52, 259, 317, 429, 1086, 1290, 1687, 2754, 2922
MlsI TGGCCA 1 cut(s) 649
MluNI TGGCCA 1 cut(s) 649
MlyI GAGTC 3 cut(s) 581, 2663, 2977
MmeI TCCRAC 2 cut(s) 2691, 2691
Mox20I TGGCCA 1 cut(s) 649
Mph1103I ATGCAT 1 cut(s) 1261
MscI TGGCCA 1 cut(s) 649
MslI CAYNNNNRTG 2 cut(s) 264, 1352
Msp20I TGGCCA 1 cut(s) 649
MspA1I CMGCKG 4 cut(s) 226, 1394, 2706, 2944
MspI CCGG 3 cut(s) 1343, 1382, 1487
MunI CAATTG 1 cut(s) 2517
Mva1269I GAATGC 3 cut(s) 1261, 1347, 1648
MvaI CCWGG 9 cut(s) 136, 740, 825, 1034, 2027, 2034, 2052, 2616, 2844
NciI CCSGG 2 cut(s) 1344, 1382
NcoI CCATGG 1 cut(s) 1926
NdeI CATATG 1 cut(s) 1219
NdeII GATC 9 cut(s) 124, 178, 328, 408, 537, 550, 1318, 1430, 2056
NlaIII CATG 8 cut(s) 185, 395, 1377, 1843, 1930, 2195, 2297, 2936
NlaIV GGNNCC 7 cut(s) 315, 428, 1684, 1685, 2031, 2665, 2856
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 3 cut(s) 1661, 2080, 2187
NsiI ATGCAT 1 cut(s) 1261
NspI RCATGY 3 cut(s) 1377, 2195, 2297
NspV TTCGAA 1 cut(s) 1766
OliI CACNNNNGTG 1 cut(s) 264
PaeR7I CTCGAG 1 cut(s) 173
PagI TCATGA 1 cut(s) 391
PceI AGGCCT 1 cut(s) 513
PcsI WCGNNNNNNNCGW 2 cut(s) 730, 997
PctI GAATGC 3 cut(s) 1261, 1347, 1648
PflMI CCANNNNNTGG 4 cut(s) 135, 656, 2426, 2615
PleI GAGTC 3 cut(s) 581, 2663, 2977
PpsI GAGTC 3 cut(s) 581, 2663, 2977
Ppu21I YACGTR 2 cut(s) 11, 1025
PshBI ATTAAT 1 cut(s) 2766
PsiI TTATAA 1 cut(s) 975
Psp124BI GAGCTC 2 cut(s) 1086, 2922
Psp6I CCWGG 9 cut(s) 134, 738, 823, 1032, 2025, 2032, 2050, 2614, 2842
PspGI CCWGG 9 cut(s) 134, 738, 823, 1032, 2025, 2032, 2050, 2614, 2842
PspN4I GGNNCC 7 cut(s) 315, 428, 1684, 1685, 2031, 2665, 2856
PspOMI GGGCCC 2 cut(s) 313, 1683
PspPI GGNCC 9 cut(s) 313, 314, 1484, 1683, 1684, 2023, 2606, 2822, 2855
PstI CTGCAG 5 cut(s) 286, 613, 847, 1254, 2949
PstNI CAGNNNCTG 1 cut(s) 1130
PsuI RGATCY 1 cut(s) 124
PvuII CAGCTG 4 cut(s) 226, 1394, 2706, 2944
RsaI GTAC 6 cut(s) 961, 1027, 1233, 1248, 2376, 2434
RsaNI GTAC 6 cut(s) 960, 1026, 1232, 1247, 2375, 2433
RseI CAYNNNNRTG 2 cut(s) 264, 1352
SacI GAGCTC 2 cut(s) 1086, 2922
SalI GTCGAC 1 cut(s) 2586
Sau3AI GATC 9 cut(s) 124, 178, 328, 408, 537, 550, 1318, 1430, 2056
Sau96I GGNCC 9 cut(s) 313, 314, 1484, 1683, 1684, 2023, 2606, 2822, 2855
ScaI AGTACT 2 cut(s) 961, 2434
SchI GAGTC 3 cut(s) 581, 2663, 2977
SduI GDGCHC 9 cut(s) 52, 259, 317, 429, 1086, 1290, 1687, 2754, 2922
SexAI ACCWGGT 1 cut(s) 2614
SfcI CTRYAG 6 cut(s) 282, 609, 843, 1250, 2449, 2945
Sfr274I CTCGAG 1 cut(s) 173
SfuI TTCGAA 1 cut(s) 1766
SinI GGWCC 2 cut(s) 2606, 2855
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 2 cut(s) 264, 1352
SmlI CTYRAG 4 cut(s) 105, 173, 616, 1639
SmoI CTYRAG 4 cut(s) 105, 173, 616, 1639
SpeI ACTAGT 1 cut(s) 2435
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 5 cut(s) 743, 893, 1706, 1984, 2115
SspI AATATT 1 cut(s) 205
SspMI CTAG 3 cut(s) 1029, 2229, 2436
SstI GAGCTC 2 cut(s) 1086, 2922
StuI AGGCCT 1 cut(s) 513
StyI CCWWGG 2 cut(s) 1926, 2872
TaaI ACNGT 6 cut(s) 993, 1183, 1565, 2528, 2536, 3012
TaiI ACGT 3 cut(s) 13, 1027, 1240
TaqI TCGA 7 cut(s) 112, 174, 724, 1766, 2587, 2654, 2913
TatI WGTACW 4 cut(s) 959, 1231, 2374, 2432
TauI GCSGC 2 cut(s) 1709, 1987
TscAI CASTG 5 cut(s) 1075, 1809, 2089, 2427, 2832
TseFI GTSAC 3 cut(s) 1661, 2080, 2187
Tsp45I GTSAC 3 cut(s) 1661, 2080, 2187
TspGWI ACGGA 2 cut(s) 989, 1711
TspRI CASTG 5 cut(s) 1075, 1809, 2089, 2427, 2832
Van91I CCANNNNNTGG 4 cut(s) 135, 656, 2426, 2615
VpaK11BI GGWCC 2 cut(s) 2606, 2855
VspI ATTAAT 1 cut(s) 2766
XagI CCTNNNNNAGG 2 cut(s) 371, 941
XapI RAATTY 4 cut(s) 114, 1114, 1778, 2272
XceI RCATGY 3 cut(s) 1377, 2195, 2297
XcmI CCANNNNNNNNNTGG 1 cut(s) 2206
XhoI CTCGAG 1 cut(s) 173
XmiI GTMKAC 3 cut(s) 2161, 2448, 2587
XspI CTAG 3 cut(s) 1029, 2229, 2436
ZrmI AGTACT 2 cut(s) 961, 2434
Zsp2I ATGCAT 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.