Rw5G031490

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008095
Physical Location & Seq
Forward (+)
51862433 .. 51869482
7050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031490.1

Sequence Viewer

Length: 2739 bp
ATGATGATCACAAAATTGGATAAAATGTCTGCTTCTCCATCATGGGAGCGTGCCCAACGTTTGTTCGACAAGATTACTGAGTTGGAGAGTAGGCGTCGGAGGTCTGCCCAAGCACGAGTTCCCTCAGATCCTAATGCCTGGCAACAGATACGTGAGAATTATGAAGCTATAATTCTTGAGGACCATGCATTTTCAGAGCAGCACAATGTTGAGTATGCTTTGTGGCAGTTACATTACAAGCGGATTGACGAATTGCGCACGCACTTCAGTGCTGCTATGGCTTCTACAGGTTCAAACTCATCCCAAGGTTTGAAGGGACCTACTCGGCCTGATCGTATTACAAAAATAAGACTGCAGTTTAAAACATTCCTTTCAGAAGCGACTGGATTTTATCATGATTTAATAGTGAAGATCAGAGCTAAGTATGGGCTTCCTCTTGGCTATTTCTCTGAGGATTCTGAAAATCGAATTGTTATGGATAAGGATGGAAAGAAATCTGCTGATATGAAGAAAGGTTTGATATCATGCCACCGCTGTTTGATTTACTTGGGTGACCTTGCACGCTACAAAGGACTGTATGGGGAGGGGGACTCCAAAACTCGTGAGTATGCTGCTGCCTCAAGTTACTACTTGCAAGCTGCATCTTGTTTGCCATCAAGTGGGAACCCTCATCATCAGCTTGCTATATTGGCTTCTTATTCTGGGGATGAGTTGGTGACTGTTTATCGATATTTTCGTAGCCTGGCCGTGGATAATCCCTTTTCAACTGCAAGGGATAACTTGATTGTGGCATTTGAGAAGAATCGTCAGAGTTACTCGCAACTGTCTGGCAATGCTAATGCTTCTGCACTCAAAGAGTTGCCTGCACGATTGACTGGCAAAGGTAGAGGGAAGGGGGAAGCAATACCTGCTAAAGATAACAACACAGAAGCAGGCCTTGTTAAGGAAAGATCATCCAGTAATCAGGAAAAATACAAAGCTTTTTCAGTTCGTTTTGTCCGTCTAAATGGCATTCTTTTTACACGTACTAGCCTGGAGACATTTGCAGAAGTTCTCTCTGTTGTAAGCAGTGGTTTATATGAGCTTATATCTTCCGGAGCAGAAGAGGAGCTGAGTCTTGGTGCTGATGCTGTTGAGAATGGACTGGTCATTGTTCGACTGGTCTCTATACTGATATTCACAGTTCATAATGTGAAAAAGGAAAGTGAAGGTCAGTCATATGCAGAAATTGTACAGCGTGCTGTTCTGCTGCAAAATGCATTTACTGCTGTCTTCGAGTTGATGGGGCATGTCTTAGAGAGATGCGTGAAGCTGGGTGATCCAACTTCAAGTTACCTGTTACCAGGCATTCTGGTTTTTGTAGAATGGTTGGCATGTTGTCCTGATGTAGCAGCTGGCAGTGATGCAGATGAAAAACAGTCTTCTGTTAGAGCAAAGTTCTGGAATGCTTGCATACACTTGTTGAATAATCTCTTATCATCTGTTCCGATGTCCATAGATGATGATGGAGATGAGACTTGCTTTAATAATATGAGCAGATATGAAGAAGGGGAAACTGAGAATCGCCTTGCTTTGTGGGAGGATTTTGAGTTGAGAGGATTCATGCCACTTCTTCCAGCACAGACAATATTGGATTTCTCGAGGAAGCATTCTTTTGGAAGTGATGGCCAGAAGGAAAAAGGTGCGCGTGTTAAAAGGATCCTGGCGGCAGGCAAGGCTTTAGCAAATGTGATCAAGGTTGATCAAAAAGCCATTTATTTTGATTCCCAGACAAAGAAATTTGTCATCGGTGTTGAGCCTTCAATGAATGGCGATTATATGCCTACCTCCTTTTTAGGCATGGCTAATTCCAATGATAATCTGGAAGAAAATCAAGTGGAGGACACCAAAAATCTGGGAGGTCCATTTCAAAAGCCAGAGTTGACCATGGATGGGGATGAGGAAGATGAAGTAATAGTTTTTAAACCAATAGTGGCTGAGAAGAGGCCTGATGTGGTAGGCACTACATGGGCTATCCCCCAGCATTTGGAACCATTTAAATCAGAAGAAATATCTCTTGCTAACAACCTGAAGAGTTTGGGATTTATGGGCAATGGGCAAGTACTGAAATCTGACCATGTATCAAGTTCGGTTCCATTCCAACAGCCTGTTAATGGTAGTACTAGTAGTATGTTTTACAGCCATGCAAAGGCTCCAGAAGCTGTGTTGCCGTTTAAGGTTGGTACTATTGCATCTTCTGGACCATTTGCGGATGGTTTGACATTGAAGACGTCATCAGCTTTGCCCGCTGCTATCCGAAAAAATCCAGTTAGTCGACCTGTTAGACACCTTGGTCCGCCACCTGGTTTTAGCCATGTTCCCACTAAACAAGTGAACGAGTCTATTTATAATTCAGAGTCAATGGGTGAGAATCCACTGATGGATGATTACAGCTGGTTGGATGGGTATCAGGTGCCATCTTCAACTAAAGGAAATGCATTCAGCAGTTCCATTAACTACTCATCTCATTCAAATCTTCATCGCGTTCCTAATGGGAATGGCTTAAGTGGAACGGTGAACTTTCCCTTTCCTGGTAAACAGGGCCCATCTATGCCATTCCAAGCAGAAAACCAGAAAAGCCTGCAAGAATTCCATATGCTCGATGATCTTAAACTTCACCATGAAATGCAGCTGCAGCAGCAGCAACAACCTGAGCAATATCAAGGACAGTCTGTCTGGACAGGCCGCTATTTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

912

Amino Acids

101.27

Weight (kDa)

6.17

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 70 - 193 2.5e-17 Telomerase activating protein Est1
EST1_DNA_bind PF10373 206 - 539 2.9e-64 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2388
AasI GACNNNNNNGTC 1 cut(s) 2331
AatII GACGTC 1 cut(s) 2273
Acc16I TGCGCA 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 916
AccB1I GGYRCC 1 cut(s) 2452
AccB7I CCANNNNNTGG 2 cut(s) 659, 2026
AccI GTMKAC 1 cut(s) 2314
AccII CGCG 2 cut(s) 1687, 2523
AccIII TCCGGA 1 cut(s) 1094
AciI CCGC 7 cut(s) 241, 532, 1706, 2249, 2286, 2336, 2725
AclI AACGTT 1 cut(s) 58
AclWI GGATC 4 cut(s) 122, 1313, 1693, 1706
AcoI YGGCCR 2 cut(s) 744, 1666
AcsI RAATTY 2 cut(s) 1778, 2627
AcuI CTGAAG 2 cut(s) 250, 2090
AcyI GRCGYC 2 cut(s) 94, 2270
AfaI GTAC 5 cut(s) 1027, 1233, 2103, 2161, 2224
AfiI CCNNNNNNNGG 9 cut(s) 659, 748, 943, 1932, 2026, 2153, 2188, 2342, 2570
AflII CTTAAG 1 cut(s) 2542
AflIII ACRYGT 1 cut(s) 1022
AhdI GACNNNNNGTC 1 cut(s) 2711
AhlI ACTAGT 1 cut(s) 2162
AjnI CCWGG 7 cut(s) 137, 741, 1032, 1342, 1701, 2341, 2569
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 267
Alw26I GTCTC 3 cut(s) 1031, 1168, 1508
AlwI GGATC 4 cut(s) 122, 1313, 1693, 1706
AlwNI CAGNNNCTG 1 cut(s) 2201
Ama87I CYCGRG 1 cut(s) 1639
Aor13HI TCCGGA 1 cut(s) 1094
AoxI GGCC 7 cut(s) 326, 744, 934, 1666, 1985, 2581, 2722
ApaI GGGCCC 1 cut(s) 2585
ApoI RAATTY 2 cut(s) 1778, 2627
AspLEI GCGC 2 cut(s) 258, 1687
AspS9I GGNCC 7 cut(s) 181, 317, 1901, 2240, 2333, 2581, 2582
AsuHPI GGTGA 6 cut(s) 563, 727, 1328, 2417, 2566, 2648
AvaI CYCGRG 1 cut(s) 1639
AvaII GGWCC 5 cut(s) 181, 317, 1901, 2240, 2333
BaeGI GKGCMC 2 cut(s) 55, 2585
BalI TGGCCA 1 cut(s) 1668
BamHI GGATCC 1 cut(s) 1698
BanI GGYRCC 1 cut(s) 2452
BanII GRGCYC 1 cut(s) 2585
BauI CACGAG 2 cut(s) 114, 600
BbsI GAAGAC 3 cut(s) 1264, 1413, 2273
BceAI ACGGC 2 cut(s) 731, 2194
BciT130I CCWGG 7 cut(s) 139, 743, 1034, 1344, 1703, 2343, 2571
BclI TGATCA 3 cut(s) 6, 1731, 1741
BcoDI GTCTC 3 cut(s) 1031, 1168, 1508
BcuI ACTAGT 1 cut(s) 2162
BfaI CTAG 2 cut(s) 1029, 2163
BfmI CTRYAG 3 cut(s) 285, 353, 2672
BfrI CTTAAG 1 cut(s) 2542
BfuAI ACCTGC 1 cut(s) 916
BmcAI AGTACT 2 cut(s) 2103, 2161
Bme1390I CCNGG 7 cut(s) 139, 743, 1034, 1344, 1703, 2343, 2571
Bme18I GGWCC 5 cut(s) 181, 317, 1901, 2240, 2333
BmeRI GACNNNNNGTC 1 cut(s) 2711
BmeT110I CYCGRG 1 cut(s) 1639
BmgT120I GGNCC 7 cut(s) 181, 317, 1901, 2240, 2333, 2581, 2582
BmiI GGNNCC 8 cut(s) 318, 665, 1700, 2031, 2133, 2193, 2454, 2583
BmrFI CCNGG 7 cut(s) 139, 743, 1034, 1344, 1703, 2343, 2571
BmsI GCATC 5 cut(s) 650, 1117, 1292, 1393, 2240
BpiI GAAGAC 3 cut(s) 1264, 1413, 2273
BplI GAGNNNNNCTC 2 cut(s) 575, 607
BpmI CTGGAG 2 cut(s) 1055, 2178
Bpu10I CCTNAGC 1 cut(s) 2691
BpuEI CTTGAG 2 cut(s) 197, 604
Bsa29I ATCGAT 1 cut(s) 727
BsaAI YACGTR 2 cut(s) 152, 1025
BsaBI GATNNNNATC 1 cut(s) 2445
BsaHI GRCGYC 2 cut(s) 94, 2270
BsaI GGTCTC 1 cut(s) 1168
BsaJI CCNNGG 4 cut(s) 304, 747, 1926, 2329
BsaWI WCCGGW 1 cut(s) 1094
Bsc4I CCNNNNNNNGG 9 cut(s) 659, 748, 943, 1932, 2026, 2153, 2188, 2342, 2570
Bse1I ACTGG 6 cut(s) 388, 880, 957, 1149, 1164, 2306
Bse3DI GCAATG 2 cut(s) 838, 2098
Bse8I GATNNNNATC 1 cut(s) 2445
BseAI TCCGGA 1 cut(s) 1094
BseBI CCWGG 7 cut(s) 139, 743, 1034, 1344, 1703, 2343, 2571
BseCI ATCGAT 1 cut(s) 727
BseDI CCNNGG 4 cut(s) 304, 747, 1926, 2329
BseGI GGATG 9 cut(s) 299, 490, 712, 953, 1936, 1942, 2257, 2428, 2446
BseJI GATNNNNATC 1 cut(s) 2445
BseLI CCNNNNNNNGG 9 cut(s) 659, 748, 943, 1932, 2026, 2153, 2188, 2342, 2570
BseMI GCAATG 2 cut(s) 838, 2098
BseMII CTCAG 7 cut(s) 69, 138, 441, 1103, 1548, 1968, 2682
BseNI ACTGG 6 cut(s) 388, 880, 957, 1149, 1164, 2306
BseRI GAGGAG 1 cut(s) 1121
BseSI GKGCMC 2 cut(s) 55, 2585
BseYI CCCAGC 2 cut(s) 1312, 2019
BsgI GTGCAG 2 cut(s) 831, 849
Bsh1236I CGCG 2 cut(s) 1687, 2523
BshFI GGCC 7 cut(s) 328, 746, 936, 1668, 1987, 2583, 2724
BshNI GGYRCC 1 cut(s) 2452
BshVI ATCGAT 1 cut(s) 727
BsiHKCI CYCGRG 1 cut(s) 1639
BsiSI CCGG 1 cut(s) 1095
BslFI GGGAC 2 cut(s) 330, 602
BslI CCNNNNNNNGG 9 cut(s) 659, 748, 943, 1932, 2026, 2153, 2188, 2342, 2570
BsmAI GTCTC 3 cut(s) 1031, 1168, 1508
BsmFI GGGAC 2 cut(s) 330, 602
BsmI GAATGC 5 cut(s) 1011, 1347, 1450, 1648, 2477
BsnI GGCC 7 cut(s) 328, 746, 936, 1668, 1987, 2583, 2724
Bso31I GGTCTC 1 cut(s) 1168
BsoBI CYCGRG 1 cut(s) 1639
Bsp120I GGGCCC 1 cut(s) 2581
Bsp1286I GDGCHC 2 cut(s) 55, 2585
Bsp13I TCCGGA 1 cut(s) 1094
Bsp1407I TGTACA 1 cut(s) 1231
Bsp19I CCATGG 1 cut(s) 1926
BspACI CCGC 7 cut(s) 241, 532, 1706, 2249, 2286, 2336, 2725
BspANI GGCC 7 cut(s) 328, 746, 936, 1668, 1987, 2583, 2724
BspCNI CTCAG 7 cut(s) 70, 137, 442, 1104, 1549, 1969, 2683
BspDI ATCGAT 1 cut(s) 727
BspEI TCCGGA 1 cut(s) 1094
BspFNI CGCG 2 cut(s) 1687, 2523
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 8 cut(s) 318, 665, 1700, 2031, 2133, 2193, 2454, 2583
BspMAI CTGCAG 2 cut(s) 357, 2676
BspMI ACCTGC 1 cut(s) 916
BspPI GGATC 4 cut(s) 122, 1313, 1693, 1706
BspT107I GGYRCC 1 cut(s) 2452
BspTI CTTAAG 1 cut(s) 2542
BspTNI GGTCTC 1 cut(s) 1168
BsrDI GCAATG 2 cut(s) 838, 2098
BsrGI TGTACA 1 cut(s) 1231
BsrI ACTGG 6 cut(s) 388, 880, 957, 1149, 1164, 2306
BssECI CCNNGG 4 cut(s) 304, 747, 1926, 2329
BssNI GRCGYC 2 cut(s) 94, 2270
BssSI CACGAG 2 cut(s) 114, 600
BssT1I CCWWGG 3 cut(s) 304, 1926, 2329
Bst2BI CACGAG 2 cut(s) 114, 600
Bst2UI CCWGG 7 cut(s) 139, 743, 1034, 1344, 1703, 2343, 2571
Bst4CI ACNGT 7 cut(s) 576, 721, 825, 1183, 1419, 2554, 2709
Bst6I CTCTTC 3 cut(s) 1098, 1976, 2066
BstACI GRCGYC 2 cut(s) 94, 2270
BstAFI CTTAAG 1 cut(s) 2542
BstAPI GCANNNNNTGC 2 cut(s) 908, 1265
BstAUI TGTACA 1 cut(s) 1231
BstBAI YACGTR 2 cut(s) 152, 1025
BstDEI CTNAG 9 cut(s) 78, 124, 420, 450, 1112, 1294, 1557, 1977, 2691
BstDSI CCRYGG 2 cut(s) 747, 1926
BstEII GGTNACC 1 cut(s) 551
BstENI CCTNNNNNAGG 1 cut(s) 941
BstF5I GGATG 9 cut(s) 299, 490, 712, 953, 1936, 1942, 2257, 2428, 2446
BstFNI CGCG 2 cut(s) 1687, 2523
BstHHI GCGC 2 cut(s) 258, 1687
BstMAI GTCTC 3 cut(s) 1031, 1168, 1508
BstNI CCWGG 7 cut(s) 139, 743, 1034, 1344, 1703, 2343, 2571
BstNSI RCATGY 2 cut(s) 1292, 1377
BstPI GGTNACC 1 cut(s) 551
BstSCI CCNGG 7 cut(s) 137, 741, 1032, 1342, 1701, 2341, 2569
BstSFI CTRYAG 3 cut(s) 285, 353, 2672
BstSLI GKGCMC 2 cut(s) 55, 2585
BstUI CGCG 2 cut(s) 1687, 2523
BstV2I GAAGAC 3 cut(s) 1264, 1413, 2273
BstX2I RGATCY 2 cut(s) 127, 1698
BstXI CCANNNNNNTGG 1 cut(s) 1894
BstYI RGATCY 2 cut(s) 127, 1698
Bsu15I ATCGAT 1 cut(s) 727
BsuRI GGCC 7 cut(s) 328, 746, 936, 1668, 1987, 2583, 2724
BsuTUI ATCGAT 1 cut(s) 727
BtgI CCRYGG 2 cut(s) 747, 1926
BtgZI GCGATG 1 cut(s) 2504
BtsCI GGATG 9 cut(s) 299, 490, 712, 953, 1936, 1942, 2257, 2428, 2446
BtsI GCAGTG 2 cut(s) 1075, 1405
BtsIMutI CAGTG 4 cut(s) 274, 1075, 1405, 2414
BveI ACCTGC 1 cut(s) 916
CaiI CAGNNNCTG 1 cut(s) 2201
CciI TCATGA 1 cut(s) 394
CfoI GCGC 2 cut(s) 258, 1687
Cfr13I GGNCC 7 cut(s) 181, 317, 1901, 2240, 2333, 2581, 2582
ClaI ATCGAT 1 cut(s) 727
CseI GACGC 1 cut(s) 83
CsiI ACCWGGT 1 cut(s) 2341
Csp6I GTAC 5 cut(s) 1026, 1232, 2102, 2160, 2223
CviQI GTAC 5 cut(s) 1026, 1232, 2102, 2160, 2223
DdeI CTNAG 9 cut(s) 78, 124, 420, 450, 1112, 1294, 1557, 1977, 2691
DraI TTTAAA 3 cut(s) 361, 1963, 2038
DrdI GACNNNNNNGTC 1 cut(s) 2331
DriI GACNNNNNGTC 1 cut(s) 2711
DseDI GACNNNNNNGTC 1 cut(s) 2331
EaeI YGGCCR 2 cut(s) 744, 1666
Eam1104I CTCTTC 3 cut(s) 1098, 1976, 2066
Eam1105I GACNNNNNGTC 1 cut(s) 2711
EarI CTCTTC 3 cut(s) 1098, 1976, 2066
EciI GGCGGA 1 cut(s) 2325
Eco130I CCWWGG 3 cut(s) 304, 1926, 2329
Eco147I AGGCCT 2 cut(s) 936, 1987
Eco24I GRGCYC 1 cut(s) 2585
Eco31I GGTCTC 1 cut(s) 1168
Eco32I GATATC 1 cut(s) 522
Eco47I GGWCC 5 cut(s) 181, 317, 1901, 2240, 2333
Eco57I CTGAAG 2 cut(s) 250, 2090
Eco88I CYCGRG 1 cut(s) 1639
Eco91I GGTNACC 1 cut(s) 551
EcoNI CCTNNNNNAGG 1 cut(s) 941
EcoO109I RGGNCCY 2 cut(s) 317, 2581
EcoO65I GGTNACC 1 cut(s) 551
EcoRI GAATTC 1 cut(s) 2627
EcoRII CCWGG 7 cut(s) 137, 741, 1032, 1342, 1701, 2341, 2569
EcoRV GATATC 1 cut(s) 522
EcoT14I CCWWGG 3 cut(s) 304, 1926, 2329
EcoT22I ATGCAT 3 cut(s) 190, 1261, 2479
EcoT38I GRGCYC 1 cut(s) 2585
ErhI CCWWGG 3 cut(s) 304, 1926, 2329
FalI AAGNNNNNCTT 2 cut(s) 921, 953
FaqI GGGAC 2 cut(s) 330, 602
FauI CCCGC 1 cut(s) 2293
FauNDI CATATG 2 cut(s) 1219, 2634
FbaI TGATCA 3 cut(s) 6, 1731, 1741
FblI GTMKAC 1 cut(s) 2314
FokI GGATG 9 cut(s) 286, 497, 719, 940, 1943, 1949, 2264, 2435, 2453
FriOI GRGCYC 1 cut(s) 2585
FspBI CTAG 2 cut(s) 1029, 2163
FspI TGCGCA 1 cut(s) 257
GlaI GCGC 2 cut(s) 257, 1686
GsaI CCCAGC 2 cut(s) 1316, 2023
GsuI CTGGAG 2 cut(s) 1055, 2178
HaeIII GGCC 7 cut(s) 328, 746, 936, 1668, 1987, 2583, 2724
HapII CCGG 1 cut(s) 1095
HgaI GACGC 1 cut(s) 83
HhaI GCGC 2 cut(s) 258, 1687
Hin1I GRCGYC 2 cut(s) 94, 2270
Hin6I GCGC 2 cut(s) 256, 1685
HinP1I GCGC 2 cut(s) 256, 1685
HincII GTYRAC 2 cut(s) 1923, 2315
HindII GTYRAC 2 cut(s) 1923, 2315
HindIII AAGCTT 1 cut(s) 978
HpaII CCGG 1 cut(s) 1095
HphI GGTGA 6 cut(s) 563, 727, 1328, 2417, 2566, 2648
Hpy166II GTNNAC 5 cut(s) 1923, 2315, 2374, 2557, 2576
Hpy8I GTNNAC 5 cut(s) 1923, 2315, 2374, 2557, 2576
Hpy99I CGWCG 1 cut(s) 99
HpyAV CCTTC 6 cut(s) 307, 886, 1202, 1541, 1666, 1809
HpyCH4III ACNGT 7 cut(s) 576, 721, 825, 1183, 1419, 2554, 2709
HpyCH4IV ACGT 4 cut(s) 58, 151, 1024, 2270
HpyF3I CTNAG 9 cut(s) 78, 124, 420, 450, 1112, 1294, 1557, 1977, 2691
HpySE526I ACGT 4 cut(s) 58, 151, 1024, 2270
Hsp92I GRCGYC 2 cut(s) 94, 2270
HspAI GCGC 2 cut(s) 256, 1685
Kpn2I TCCGGA 1 cut(s) 1094
Ksp22I TGATCA 3 cut(s) 6, 1731, 1741
LmnI GCTCC 4 cut(s) 46, 1097, 1108, 2197
LweI GCATC 5 cut(s) 650, 1117, 1292, 1393, 2240
MabI ACCWGGT 1 cut(s) 2341
MaeI CTAG 2 cut(s) 1029, 2163
MaeII ACGT 4 cut(s) 58, 151, 1024, 2270
MaeIII GTNAC 7 cut(s) 228, 551, 623, 715, 812, 1331, 1338
MflI RGATCY 2 cut(s) 127, 1698
MhlI GDGCHC 2 cut(s) 55, 2585
MlsI TGGCCA 1 cut(s) 1668
MluNI TGGCCA 1 cut(s) 1668
MlyI GAGTC 4 cut(s) 584, 1123, 2387, 2405
MmeI TCCRAC 5 cut(s) 63, 77, 1346, 2164, 2418
Mox20I TGGCCA 1 cut(s) 1668
Mph1103I ATGCAT 3 cut(s) 190, 1261, 2479
MroI TCCGGA 1 cut(s) 1094
MscI TGGCCA 1 cut(s) 1668
MslI CAYNNNNRTG 1 cut(s) 267
Msp20I TGGCCA 1 cut(s) 1668
MspA1I CMGCKG 5 cut(s) 534, 1394, 2288, 2433, 2671
MspCI CTTAAG 1 cut(s) 2542
MspI CCGG 1 cut(s) 1095
MspR9I CCNGG 7 cut(s) 139, 743, 1034, 1344, 1703, 2343, 2571
Mva1269I GAATGC 5 cut(s) 1011, 1347, 1450, 1648, 2477
MvaI CCWGG 7 cut(s) 139, 743, 1034, 1344, 1703, 2343, 2571
MvnI CGCG 2 cut(s) 1687, 2523
NcoI CCATGG 1 cut(s) 1926
NdeI CATATG 2 cut(s) 1219, 2634
NlaIV GGNNCC 8 cut(s) 318, 665, 1700, 2031, 2133, 2193, 2454, 2583
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 551, 715
NsbI TGCGCA 1 cut(s) 257
NsiI ATGCAT 3 cut(s) 190, 1261, 2479
NspI RCATGY 2 cut(s) 1292, 1377
OliI CACNNNNGTG 1 cut(s) 267
PaeR7I CTCGAG 1 cut(s) 1639
PagI TCATGA 1 cut(s) 394
PceI AGGCCT 2 cut(s) 936, 1987
PcsI WCGNNNNNNNCGW 3 cut(s) 331, 733, 997
PctI GAATGC 5 cut(s) 1011, 1347, 1450, 1648, 2477
PfeI GAWTC 6 cut(s) 455, 802, 1561, 1599, 1763, 2410
PflMI CCANNNNNTGG 2 cut(s) 659, 2026
PleI GAGTC 4 cut(s) 584, 1122, 2386, 2404
PpsI GAGTC 4 cut(s) 584, 1122, 2386, 2404
Ppu21I YACGTR 2 cut(s) 152, 1025
PpuMI RGGWCCY 1 cut(s) 317
PsiI TTATAA 1 cut(s) 2388
Psp1406I AACGTT 1 cut(s) 58
Psp5II RGGWCCY 1 cut(s) 317
Psp6I CCWGG 7 cut(s) 137, 741, 1032, 1342, 1701, 2341, 2569
PspEI GGTNACC 1 cut(s) 551
PspFI CCCAGC 2 cut(s) 1312, 2019
PspGI CCWGG 7 cut(s) 137, 741, 1032, 1342, 1701, 2341, 2569
PspN4I GGNNCC 8 cut(s) 318, 665, 1700, 2031, 2133, 2193, 2454, 2583
PspOMI GGGCCC 1 cut(s) 2581
PspPI GGNCC 7 cut(s) 181, 317, 1901, 2240, 2333, 2581, 2582
PspPPI RGGWCCY 1 cut(s) 317
PstI CTGCAG 2 cut(s) 357, 2676
PstNI CAGNNNCTG 1 cut(s) 2201
PsuI RGATCY 2 cut(s) 127, 1698
PvuII CAGCTG 3 cut(s) 1394, 2433, 2671
RsaI GTAC 5 cut(s) 1027, 1233, 2103, 2161, 2224
RsaNI GTAC 5 cut(s) 1026, 1232, 2102, 2160, 2223
RseI CAYNNNNRTG 1 cut(s) 267
SalI GTCGAC 1 cut(s) 2313
Sau96I GGNCC 7 cut(s) 181, 317, 1901, 2240, 2333, 2581, 2582
ScaI AGTACT 2 cut(s) 2103, 2161
SchI GAGTC 4 cut(s) 584, 1123, 2387, 2405
ScrFI CCNGG 7 cut(s) 139, 743, 1034, 1344, 1703, 2343, 2571
SduI GDGCHC 2 cut(s) 55, 2585
SexAI ACCWGGT 1 cut(s) 2341
SfaNI GCATC 5 cut(s) 650, 1117, 1292, 1393, 2240
SfcI CTRYAG 3 cut(s) 285, 353, 2672
Sfr274I CTCGAG 1 cut(s) 1639
SinI GGWCC 5 cut(s) 181, 317, 1901, 2240, 2333
SlaI CTCGAG 1 cut(s) 1639
SmiI ATTTAAAT 1 cut(s) 2038
SmiMI CAYNNNNRTG 1 cut(s) 267
SmlI CTYRAG 4 cut(s) 176, 619, 1639, 2542
SmoI CTYRAG 4 cut(s) 176, 619, 1639, 2542
SpeI ACTAGT 1 cut(s) 2162
SseBI AGGCCT 2 cut(s) 936, 1987
SsiI CCGC 7 cut(s) 241, 532, 1706, 2249, 2286, 2336, 2725
SspI AATATT 1 cut(s) 1629
SspMI CTAG 2 cut(s) 1029, 2163
StuI AGGCCT 2 cut(s) 936, 1987
StyD4I CCNGG 7 cut(s) 137, 741, 1032, 1342, 1701, 2341, 2569
StyI CCWWGG 3 cut(s) 304, 1926, 2329
SwaI ATTTAAAT 1 cut(s) 2038
TaaI ACNGT 7 cut(s) 576, 721, 825, 1183, 1419, 2554, 2709
TaiI ACGT 4 cut(s) 61, 154, 1027, 2273
TaqI TCGA 8 cut(s) 66, 466, 727, 1156, 1275, 1640, 2314, 2640
TatI WGTACW 3 cut(s) 1231, 2101, 2159
TauI GCSGC 2 cut(s) 1709, 2727
TfiI GAWTC 6 cut(s) 455, 802, 1561, 1599, 1763, 2410
TscAI CASTG 4 cut(s) 274, 1075, 1405, 2421
TseFI GTSAC 2 cut(s) 551, 715
Tsp45I GTSAC 2 cut(s) 551, 715
TspGWI ACGGA 1 cut(s) 989
TspRI CASTG 4 cut(s) 274, 1075, 1405, 2421
Van91I CCANNNNNTGG 2 cut(s) 659, 2026
Vha464I CTTAAG 1 cut(s) 2542
VpaK11BI GGWCC 5 cut(s) 181, 317, 1901, 2240, 2333
XagI CCTNNNNNAGG 1 cut(s) 941
XapI RAATTY 2 cut(s) 1778, 2627
XceI RCATGY 2 cut(s) 1292, 1377
XcmI CCANNNNNNNNNTGG 1 cut(s) 1858
XhoI CTCGAG 1 cut(s) 1639
XmiI GTMKAC 1 cut(s) 2314
XspI CTAG 2 cut(s) 1029, 2163
ZraI GACGTC 1 cut(s) 2271
ZrmI AGTACT 2 cut(s) 2103, 2161
Zsp2I ATGCAT 3 cut(s) 190, 1261, 2479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.