MD11G1184900.v1.1

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008095
Physical Location & Seq
Forward (+)
24749828 .. 24757197
7370 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1184900.v1.1.491

Sequence Viewer

Length: 3012 bp
ATGATGATTCCACAGATGGATAAAATGTCTGCTCCTTCATCAAGGGAGCGTGCCCAACGTCTATATGACAAGATTCTTGAGTTGGAGAATAGGCGTCGGAGGTCTGCCCAAGCACGAATTCCATCAGATCCCAATGCTTGGCAACAGATGCGTGAGAATTATGAAGCAATAATTCTTGAGGACCATGCTTTCTCCGAGCAGCACAATATCGAGTATGCTTTGTGGCAGTTACATTACAAGCGCATTGAGGAATTGCGAGCACACTTCAGTGCTGCTACAGCTTCTGCAGGTTCAAACACATCTCAGGGTGTGAAGGGGCCAATTCGGCCTGATCGAGTTACAAAAATAAGACTGCAGTTTAAAACATTCCTTTCAGAAGCAACTGGATTTTATCATGATTTAATAGTGAAGATCAGAGCGAAATATGGGCTTCCTCTTGGTTATTTCTCTGAAGATTCTGAAAACCAGATTGTTATGGACAAGGATGGAAAGAAAGGTCTAATATCGTGCCACAGATGCTTGATATATTTGGGTGACCTTGCACGCTACAAAGGATTGTATGGGGAAGGGGACTCAAAAACTCGTGAGTACGCTGCAGCCTCAAGTTACTACATGCAAGCTGCATCTCTCTGGCCATCCAGTGGGAACCCCCATCACCAGCTTGCTATATTGGCTTCCTATTCGGGGGATGAGTTGGTGGCTCTTTATCGATATTTTCGTAGCCTGGCGGTGGATAGTCCCTTTTCAACCGCAAGAGATAACTTGATTGTTGCATTTGAGAAGAATCGTCAGAGTTACTCTCAACTGTCTGGGAACACTAATGCTTCTGCAGTCAAAGAGTTGCCTGCACGGTTGGCAGGCATAGGTAGAGGAAAGGGGGAAATAATACCTGCATCTAAAGATAACAATACCAAAGTAAGCCTTGTTAAGGAAAGAGCATCCAGTACTCAGGAGACCTATAAGGCTTTTTGCATCCGGTTTGTCCGTCTGAATGGCATTCTTTTCACGCGTACTAGCCTGGAGACATTTGCAGAAGTTCTATCTGTGGTTAGCAGTGGCTTATGTGAGCTTCTGTCTAATGGAGCAGAAGAGGTGCTGAATTTTGGTGCAGATGCTGTTGAGAATGGACTTGCTATTGTTAGACTTATCTCCATATTGATATTCACAATTCATAATGTGAAAAAGGAAAGCGAAGGTCAGACATATGCAGAAATTGTACAACGTGCTGTCCTACTGCGGAATGCATTTACTGCTGTTTTTGAGTTGGCGGGGCACATCTTAGAGAGATGCGTGCAGTTGTGTGATCCGTCTTCAAGTTTCCTGTTACCAGGTATTCTTGTTTTTGTAGAATGGTTGGCATGTTGTCCTGATGTTGCAGCTGGAAGTGATTCAGACGAAAAACAGTTGTCTGTTAGATCCAAATTCTGGAATGTTTGCATATCCTTCTTCAATAATCTCTTGTCGACTGGGCCAATGTCGATAGATGATGAAGATGAGACTTGCTTTAATAACATGAGCAGATATGAAGAAGGTGAAACTGAAAATCGTCTTGCTTTGTTGGAGGATTTTGAGTTGAGAGGATTTATTCCACTAATTCCAGCACAAACAATATTGGACTTCTCGAGGAAGCATTCTTTTGGAAGTGATGGCCATAAGGAAAAAGGGGCCCGTGTTAAAAGGATTCTGGCAGCAGGCAAAGCTCTTGCAAATGTGGTTAAGGTTGACCAGAAAGCAGTATATTTTGATTCGAAGTTAAAGAAATTTGTCATAGGCTTTGAACCTCAAGTGCTAAATGATATCGTTCCTACCTCCTATCGACATATGGCTACTGAAGATGATAATCTGCAAGAAAATCAAGCAGAGAGCACAATCAATTTGGTGGGAGCTGTATATCCAAAGACAGAGTTATCCATGGAAGGGGACGAGGAAGATGAAGTAATAGTATTTAAGCCAGTAGTGGCTGAGAAGCGACCTGATGCCGCAAGCACTACGTGGACAGCGTATGACGGCCTACAGACTGGTAAAAATGCTTCTGCAGGTGATCAAACAGTTAATGGAACTTATGGTTCTGCCACTTCGGATAATCTACACCATCAAAATGCTTTTAGGGCTGATTCACAAATACCTGTATCTTTTGCTAATGGTATACACCAGCACTGGCAACCAATCCAGTCACATGCTTCCAAGTTTTCAGTGGAAACTGGTTTTGGTGTTGGTTCACAACTACCTGCATCAAATGCCAATACCATCCCCCAGAATTTACAACCAATTCAATCAAATGCTTTGAATCCGGCAATGGAGGAAGAAATGTCTCTTGCTAATAGCTTAAGGAGTATGGGATTTATGGGAAATGGGCATGCACTAAAATCTGAGCCTGTGGCAGTTTCTGTTCCATACCAACTCCCAGTGAATGGTAGTGCTAGTGGTATGTTTTACAGCCACACGAAAGCTTCAGAAGCTTTTTTGCCATATAAAGTTGATGCAATTGCTGATGGGTTGACTGTAAAGACATCATCAGCTCTGCCTACAGGTATCCGAAAAAGTCCAGTAAGTCGACCTGTTAGGCACCTTGGTCCACCACCCGGTTTTAGCCACGTTCCTCCTAAAAATGCAAACGAATCAATTTCTGGTTCAGATTCAATGAGTGAGAGTCTAGTTATGGATGATTACAGCTGGTTGGATGGATATCAGATGCCATCTTCAACAAAAGTAAATGGGCTGAATAATTATGTTAATTACTCATCTCATTCCAATCCCCATAGCTTCACAAACAGTAATGGCTTAAGTGGAGCAGTGAACTTCCCCTTTCCTGGTAAACAGGGTACACCTATGCAACTACCAGGGGAAAACCAGAAAAGCTGGCAAGATTTCCAGAGGCTTGATGACCTCAAGCTACACCATGAAATGCAGCTGCAGCAACAGCAACAACTTGTAAATGGAAATCAGCACCCAAATCCACAGCCTGAGCAATGTCAAGGACAGTCTCTCTGGATGGGCCGCCATTTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

1004

Amino Acids

111.0

Weight (kDa)

6.09

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 70 - 187 2.3e-16 Telomerase activating protein Est1
EST1_DNA_bind PF10373 200 - 534 3.2e-63 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2027
Acc36I ACCTGC 4 cut(s) 278, 898, 2027, 2236
AccB1I GGYRCC 1 cut(s) 2565
AccB7I CCANNNNNTGG 4 cut(s) 138, 641, 1425, 2411
AccI GTMKAC 3 cut(s) 1463, 2146, 2554
AccII CGCG 1 cut(s) 1009
AciI CCGC 6 cut(s) 728, 750, 1237, 1268, 1980, 2998
AclWI GGATC 3 cut(s) 122, 1298, 1410
AcoI YGGCCR 2 cut(s) 632, 1648
AcsI RAATTY 5 cut(s) 117, 1099, 1421, 1760, 2257
AcuI CTGAAG 4 cut(s) 250, 471, 1851, 2436
AcyI GRCGYC 1 cut(s) 94
AdeI CACNNNGTG 1 cut(s) 1992
AfaI GTAC 5 cut(s) 590, 946, 1012, 1218, 2824
AflII CTTAAG 2 cut(s) 2326, 2782
AflIII ACRYGT 1 cut(s) 1007
AgsI TTSAA 9 cut(s) 294, 747, 1314, 1450, 1778, 2273, 2287, 2640, 2703
AjnI CCWGG 5 cut(s) 723, 1017, 1327, 2809, 2839
AjuI GAANNNNNNNTTGG 4 cut(s) 102, 134, 2190, 2222
AleI CACNNNNGTG 1 cut(s) 267
Alw21I GWGCWC 2 cut(s) 262, 1868
Alw26I GTCTC 5 cut(s) 947, 1016, 1490, 2316, 2988
AlwI GGATC 3 cut(s) 122, 1298, 1410
AlwNI CAGNNNCTG 3 cut(s) 284, 1115, 2387
Ama87I CYCGRG 1 cut(s) 1621
AoxI GGCC 8 cut(s) 317, 326, 632, 1469, 1648, 1665, 2008, 2995
ApaI GGGCCC 1 cut(s) 1669
ApoI RAATTY 5 cut(s) 117, 1099, 1421, 1760, 2257
Asp700I GAANNNNTTC 1 cut(s) 1387
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 7 cut(s) 181, 317, 1469, 1665, 1666, 2573, 2995
AsuC2I CCSGG 1 cut(s) 2583
AsuHPI GGTGA 4 cut(s) 545, 647, 1544, 2051
AsuII TTCGAA 1 cut(s) 1748
AvaI CYCGRG 1 cut(s) 1621
AvaII GGWCC 2 cut(s) 181, 2573
BaeGI GKGCMC 3 cut(s) 55, 1275, 1669
BalI TGGCCA 2 cut(s) 634, 1650
BanI GGYRCC 1 cut(s) 2565
BanII GRGCYC 1 cut(s) 1669
BarI GAAGNNNNNNTAC 2 cut(s) 2014, 2046
BauI CACGAG 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 1302
Bbv12I GWGCWC 2 cut(s) 262, 1868
BceAI ACGGC 1 cut(s) 2023
BciT130I CCWGG 5 cut(s) 725, 1019, 1329, 2811, 2841
BciVI GTATCC 1 cut(s) 2543
BclI TGATCA 1 cut(s) 2041
BcnI CCSGG 1 cut(s) 2583
BcoDI GTCTC 5 cut(s) 947, 1016, 1490, 2316, 2988
BfaI CTAG 3 cut(s) 1014, 2421, 2654
BfmI CTRYAG 9 cut(s) 276, 285, 353, 594, 828, 2012, 2034, 2526, 2912
BfrI CTTAAG 2 cut(s) 2326, 2782
BfuAI ACCTGC 4 cut(s) 278, 898, 2027, 2236
BfuI GTATCC 1 cut(s) 2543
BglI GCCNNNNNGGC 1 cut(s) 325
BmcAI AGTACT 1 cut(s) 946
Bme1390I CCNGG 6 cut(s) 725, 1019, 1329, 2583, 2811, 2841
Bme18I GGWCC 2 cut(s) 181, 2573
BmeT110I CYCGRG 1 cut(s) 1621
BmgT120I GGNCC 7 cut(s) 181, 317, 1469, 1665, 1666, 2573, 2995
BmiI GGNNCC 5 cut(s) 318, 647, 1666, 1667, 2567
BmrFI CCNGG 6 cut(s) 725, 1019, 1329, 2583, 2811, 2841
BmrI ACTGGG 2 cut(s) 1476, 2399
BmuI ACTGGG 2 cut(s) 1476, 2399
BpiI GAAGAC 1 cut(s) 1302
BplI GAGNNNNNCTC 2 cut(s) 784, 816
BpmI CTGGAG 1 cut(s) 1040
Bpu10I CCTNAGC 1 cut(s) 2964
Bpu14I TTCGAA 1 cut(s) 1748
BpuEI CTTGAG 5 cut(s) 98, 197, 586, 1767, 2873
BpuMI CCSGG 1 cut(s) 2583
Bsa29I ATCGAT 1 cut(s) 709
BsaAI YACGTR 1 cut(s) 1992
BsaBI GATNNNNATC 2 cut(s) 1839, 2685
BsaHI GRCGYC 1 cut(s) 94
BsaI GGTCTC 1 cut(s) 947
BsaJI CCNNGG 3 cut(s) 1911, 2569, 2840
BsaWI WCCGGW 1 cut(s) 975
Bse3DI GCAATG 2 cut(s) 2301, 2975
Bse8I GATNNNNATC 2 cut(s) 1839, 2685
BseBI CCWGG 5 cut(s) 725, 1019, 1329, 2811, 2841
BseCI ATCGAT 1 cut(s) 709
BseDI CCNNGG 3 cut(s) 1911, 2569, 2840
BseGI GGATG 9 cut(s) 490, 635, 694, 938, 972, 2247, 2668, 2686, 2997
BseJI GATNNNNATC 2 cut(s) 1839, 2685
BseMI GCAATG 2 cut(s) 2301, 2975
BseMII CTCAG 5 cut(s) 317, 962, 1953, 2361, 2955
BseSI GKGCMC 3 cut(s) 55, 1275, 1669
BsgI GTGCAG 3 cut(s) 831, 1128, 1313
Bsh1236I CGCG 1 cut(s) 1009
BshFI GGCC 8 cut(s) 319, 328, 634, 1471, 1650, 1667, 2010, 2997
BshNI GGYRCC 1 cut(s) 2565
BshVI ATCGAT 1 cut(s) 709
BsiHKAI GWGCWC 2 cut(s) 262, 1868
BsiHKCI CYCGRG 1 cut(s) 1621
BsiSI CCGG 3 cut(s) 976, 2291, 2583
BslFI GGGAC 3 cut(s) 584, 723, 1934
BsmAI GTCTC 5 cut(s) 947, 1016, 1490, 2316, 2988
BsmFI GGGAC 3 cut(s) 584, 723, 1934
BsmI GAATGC 3 cut(s) 996, 1246, 1630
BsnI GGCC 8 cut(s) 319, 328, 634, 1471, 1650, 1667, 2010, 2997
Bso31I GGTCTC 1 cut(s) 947
BsoBI CYCGRG 1 cut(s) 1621
Bsp119I TTCGAA 1 cut(s) 1748
Bsp120I GGGCCC 1 cut(s) 1665
Bsp1286I GDGCHC 5 cut(s) 55, 262, 1275, 1669, 1868
Bsp1407I TGTACA 1 cut(s) 1216
Bsp143I GATC 6 cut(s) 127, 331, 411, 1303, 1415, 2041
Bsp19I CCATGG 1 cut(s) 1911
BspACI CCGC 6 cut(s) 728, 750, 1237, 1268, 1980, 2998
BspANI GGCC 8 cut(s) 319, 328, 634, 1471, 1650, 1667, 2010, 2997
BspCNI CTCAG 5 cut(s) 316, 961, 1954, 2362, 2956
BspDI ATCGAT 1 cut(s) 709
BspFNI CGCG 1 cut(s) 1009
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 5 cut(s) 318, 647, 1666, 1667, 2567
BspMAI CTGCAG 6 cut(s) 289, 357, 598, 832, 2038, 2916
BspMI ACCTGC 4 cut(s) 278, 898, 2027, 2236
BspPI GGATC 3 cut(s) 122, 1298, 1410
BspT104I TTCGAA 1 cut(s) 1748
BspT107I GGYRCC 1 cut(s) 2565
BspTI CTTAAG 2 cut(s) 2326, 2782
BspTNI GGTCTC 1 cut(s) 947
BsrDI GCAATG 2 cut(s) 2301, 2975
BsrGI TGTACA 1 cut(s) 1216
BssECI CCNNGG 3 cut(s) 1911, 2569, 2840
BssMI GATC 6 cut(s) 127, 331, 411, 1303, 1415, 2041
BssNAI GTATAC 1 cut(s) 2147
BssNI GRCGYC 1 cut(s) 94
BssSI CACGAG 1 cut(s) 582
BssT1I CCWWGG 2 cut(s) 1911, 2569
Bst1107I GTATAC 1 cut(s) 2147
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 5 cut(s) 725, 1019, 1329, 2811, 2841
Bst4CI ACNGT 7 cut(s) 807, 852, 1404, 2050, 2503, 2774, 2982
Bst6I CTCTTC 1 cut(s) 1083
BstACI GRCGYC 1 cut(s) 94
BstAFI CTTAAG 2 cut(s) 2326, 2782
BstAPI GCANNNNNTGC 3 cut(s) 148, 1250, 2237
BstAUI TGTACA 1 cut(s) 1216
BstBAI YACGTR 1 cut(s) 1992
BstBI TTCGAA 1 cut(s) 1748
BstDEI CTNAG 6 cut(s) 303, 948, 1279, 1962, 2370, 2964
BstDSI CCRYGG 1 cut(s) 1911
BstEII GGTNACC 1 cut(s) 533
BstENI CCTNNNNNAGG 1 cut(s) 926
BstF5I GGATG 9 cut(s) 490, 635, 694, 938, 972, 2247, 2668, 2686, 2997
BstFNI CGCG 1 cut(s) 1009
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 6 cut(s) 130, 334, 414, 1306, 1418, 2044
BstMAI GTCTC 5 cut(s) 947, 1016, 1490, 2316, 2988
BstMBI GATC 6 cut(s) 127, 331, 411, 1303, 1415, 2041
BstNI CCWGG 5 cut(s) 725, 1019, 1329, 2811, 2841
BstNSI RCATGY 4 cut(s) 616, 1362, 2180, 2360
BstPI GGTNACC 1 cut(s) 533
BstSCI CCNGG 6 cut(s) 723, 1017, 1327, 2581, 2809, 2839
BstSFI CTRYAG 9 cut(s) 276, 285, 353, 594, 828, 2012, 2034, 2526, 2912
BstSLI GKGCMC 3 cut(s) 55, 1275, 1669
BstUI CGCG 1 cut(s) 1009
BstV2I GAAGAC 1 cut(s) 1302
BstX2I RGATCY 2 cut(s) 127, 1415
BstYI RGATCY 2 cut(s) 127, 1415
BstZ17I GTATAC 1 cut(s) 2147
Bsu15I ATCGAT 1 cut(s) 709
BsuI GTATCC 1 cut(s) 2543
BsuRI GGCC 8 cut(s) 319, 328, 634, 1471, 1650, 1667, 2010, 2997
BsuTUI ATCGAT 1 cut(s) 709
BtgI CCRYGG 1 cut(s) 1911
BtsCI GGATG 9 cut(s) 490, 635, 694, 938, 972, 2247, 2668, 2686, 2997
BtsI GCAGTG 2 cut(s) 1060, 2799
BtsIMutI CAGTG 7 cut(s) 274, 646, 1060, 2155, 2199, 2412, 2799
BveI ACCTGC 4 cut(s) 278, 898, 2027, 2236
CaiI CAGNNNCTG 3 cut(s) 284, 1115, 2387
CciI TCATGA 1 cut(s) 394
CfoI GCGC 1 cut(s) 243
Cfr13I GGNCC 7 cut(s) 181, 317, 1469, 1665, 1666, 2573, 2995
ClaI ATCGAT 1 cut(s) 709
CseI GACGC 1 cut(s) 83
CsiI ACCWGGT 1 cut(s) 1327
Csp6I GTAC 5 cut(s) 589, 945, 1011, 1217, 2823
CviAII CATG 9 cut(s) 185, 395, 613, 1359, 1513, 1912, 2177, 2357, 2900
CviQI GTAC 5 cut(s) 589, 945, 1011, 1217, 2823
DdeI CTNAG 6 cut(s) 303, 948, 1279, 1962, 2370, 2964
DpnI GATC 6 cut(s) 129, 333, 413, 1305, 1417, 2043
DpnII GATC 6 cut(s) 127, 331, 411, 1303, 1415, 2041
DraI TTTAAA 1 cut(s) 361
DraIII CACNNNGTG 1 cut(s) 1992
EaeI YGGCCR 2 cut(s) 632, 1648
Eam1104I CTCTTC 1 cut(s) 1083
EarI CTCTTC 1 cut(s) 1083
Eco130I CCWWGG 2 cut(s) 1911, 2569
Eco24I GRGCYC 1 cut(s) 1669
Eco31I GGTCTC 1 cut(s) 947
Eco32I GATATC 2 cut(s) 1798, 2687
Eco47I GGWCC 2 cut(s) 181, 2573
Eco57I CTGAAG 4 cut(s) 250, 471, 1851, 2436
Eco88I CYCGRG 1 cut(s) 1621
Eco91I GGTNACC 1 cut(s) 533
EcoNI CCTNNNNNAGG 1 cut(s) 926
EcoO109I RGGNCCY 1 cut(s) 1665
EcoO65I GGTNACC 1 cut(s) 533
EcoRI GAATTC 1 cut(s) 117
EcoRII CCWGG 5 cut(s) 723, 1017, 1327, 2809, 2839
EcoRV GATATC 2 cut(s) 1798, 2687
EcoT14I CCWWGG 2 cut(s) 1911, 2569
EcoT22I ATGCAT 1 cut(s) 1246
EcoT38I GRGCYC 1 cut(s) 1669
ErhI CCWWGG 2 cut(s) 1911, 2569
FaeI CATG 9 cut(s) 188, 398, 616, 1362, 1516, 1915, 2180, 2360, 2903
FalI AAGNNNNNCTT 2 cut(s) 906, 938
FaqI GGGAC 3 cut(s) 584, 723, 1934
FatI CATG 9 cut(s) 184, 394, 612, 1358, 1512, 1911, 2176, 2356, 2899
FauI CCCGC 1 cut(s) 1261
FauNDI CATATG 2 cut(s) 1204, 1821
FbaI TGATCA 1 cut(s) 2041
FblI GTMKAC 3 cut(s) 1463, 2146, 2554
FokI GGATG 9 cut(s) 497, 622, 701, 925, 959, 2234, 2675, 2693, 3004
FriOI GRGCYC 1 cut(s) 1669
FspBI CTAG 3 cut(s) 1014, 2421, 2654
GlaI GCGC 1 cut(s) 242
GsuI CTGGAG 1 cut(s) 1040
HaeIII GGCC 8 cut(s) 319, 328, 634, 1471, 1650, 1667, 2010, 2997
HapII CCGG 3 cut(s) 976, 2291, 2583
HgaI GACGC 1 cut(s) 83
HhaI GCGC 1 cut(s) 243
Hin1I GRCGYC 1 cut(s) 94
Hin1II CATG 9 cut(s) 188, 398, 616, 1362, 1516, 1915, 2180, 2360, 2903
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HincII GTYRAC 4 cut(s) 1464, 1723, 2499, 2555
HindII GTYRAC 4 cut(s) 1464, 1723, 2499, 2555
HindIII AAGCTT 2 cut(s) 2448, 2457
HpaII CCGG 3 cut(s) 976, 2291, 2583
HphI GGTGA 4 cut(s) 545, 647, 1544, 2051
Hpy99I CGWCG 1 cut(s) 99
HpyAV CCTTC 7 cut(s) 45, 307, 560, 1187, 1453, 1523, 1910
HpyCH4III ACNGT 7 cut(s) 807, 852, 1404, 2050, 2503, 2774, 2982
HpyCH4IV ACGT 4 cut(s) 58, 1222, 1991, 2595
HpyF3I CTNAG 6 cut(s) 303, 948, 1279, 1962, 2370, 2964
HpySE526I ACGT 4 cut(s) 58, 1222, 1991, 2595
Hsp92I GRCGYC 1 cut(s) 94
Hsp92II CATG 9 cut(s) 188, 398, 616, 1362, 1516, 1915, 2180, 2360, 2903
HspAI GCGC 1 cut(s) 241
Ksp22I TGATCA 1 cut(s) 2041
Kzo9I GATC 6 cut(s) 127, 331, 411, 1303, 1415, 2041
LmnI GCTCC 5 cut(s) 37, 46, 1082, 1883, 2789
MabI ACCWGGT 1 cut(s) 1327
MaeI CTAG 3 cut(s) 1014, 2421, 2654
MaeII ACGT 4 cut(s) 58, 1222, 1991, 2595
MaeIII GTNAC 7 cut(s) 228, 337, 533, 605, 794, 1323, 2172
MalI GATC 6 cut(s) 129, 333, 413, 1305, 1417, 2043
MboI GATC 6 cut(s) 127, 331, 411, 1303, 1415, 2041
MfeI CAATTG 1 cut(s) 2484
MflI RGATCY 2 cut(s) 127, 1415
MhlI GDGCHC 5 cut(s) 55, 262, 1275, 1669, 1868
MlsI TGGCCA 2 cut(s) 634, 1650
MluI ACGCGT 1 cut(s) 1007
MluNI TGGCCA 2 cut(s) 634, 1650
MlyI GAGTC 2 cut(s) 566, 2659
MmeI TCCRAC 4 cut(s) 63, 77, 1539, 2658
Mox20I TGGCCA 2 cut(s) 634, 1650
Mph1103I ATGCAT 1 cut(s) 1246
MroXI GAANNNNTTC 1 cut(s) 1387
MscI TGGCCA 2 cut(s) 634, 1650
MslI CAYNNNNRTG 2 cut(s) 267, 2097
Msp20I TGGCCA 2 cut(s) 634, 1650
MspA1I CMGCKG 3 cut(s) 1379, 2673, 2911
MspCI CTTAAG 2 cut(s) 2326, 2782
MspI CCGG 3 cut(s) 976, 2291, 2583
MspR9I CCNGG 6 cut(s) 725, 1019, 1329, 2583, 2811, 2841
MunI CAATTG 1 cut(s) 2484
Mva1269I GAATGC 3 cut(s) 996, 1246, 1630
MvaI CCWGG 5 cut(s) 725, 1019, 1329, 2811, 2841
MvnI CGCG 1 cut(s) 1009
NciI CCSGG 1 cut(s) 2583
NcoI CCATGG 1 cut(s) 1911
NdeI CATATG 2 cut(s) 1204, 1821
NdeII GATC 6 cut(s) 127, 331, 411, 1303, 1415, 2041
NlaIII CATG 9 cut(s) 188, 398, 616, 1362, 1516, 1915, 2180, 2360, 2903
NlaIV GGNNCC 5 cut(s) 318, 647, 1666, 1667, 2567
NmuCI GTSAC 2 cut(s) 533, 2172
NsiI ATGCAT 1 cut(s) 1246
NspI RCATGY 4 cut(s) 616, 1362, 2180, 2360
NspV TTCGAA 1 cut(s) 1748
OliI CACNNNNGTG 1 cut(s) 267
PaeI GCATGC 1 cut(s) 2360
PaeR7I CTCGAG 1 cut(s) 1621
PagI TCATGA 1 cut(s) 394
PaqCI CACCTGC 1 cut(s) 2027
PcsI WCGNNNNNNNCGW 3 cut(s) 331, 715, 1997
PctI GAATGC 3 cut(s) 996, 1246, 1630
PdmI GAANNNNTTC 1 cut(s) 1387
PflMI CCANNNNNTGG 4 cut(s) 138, 641, 1425, 2411
PleI GAGTC 2 cut(s) 566, 2658
PpsI GAGTC 2 cut(s) 566, 2658
Ppu21I YACGTR 1 cut(s) 1992
Psp6I CCWGG 5 cut(s) 723, 1017, 1327, 2809, 2839
PspEI GGTNACC 1 cut(s) 533
PspGI CCWGG 5 cut(s) 723, 1017, 1327, 2809, 2839
PspN4I GGNNCC 5 cut(s) 318, 647, 1666, 1667, 2567
PspOMI GGGCCC 1 cut(s) 1665
PspPI GGNCC 7 cut(s) 181, 317, 1469, 1665, 1666, 2573, 2995
PstI CTGCAG 6 cut(s) 289, 357, 598, 832, 2038, 2916
PstNI CAGNNNCTG 3 cut(s) 284, 1115, 2387
PsuI RGATCY 2 cut(s) 127, 1415
PvuII CAGCTG 3 cut(s) 1379, 2673, 2911
RsaI GTAC 5 cut(s) 590, 946, 1012, 1218, 2824
RsaNI GTAC 5 cut(s) 589, 945, 1011, 1217, 2823
RseI CAYNNNNRTG 2 cut(s) 267, 2097
SalI GTCGAC 2 cut(s) 1462, 2553
Sau3AI GATC 6 cut(s) 127, 331, 411, 1303, 1415, 2041
Sau96I GGNCC 7 cut(s) 181, 317, 1469, 1665, 1666, 2573, 2995
ScaI AGTACT 1 cut(s) 946
SchI GAGTC 2 cut(s) 566, 2659
ScrFI CCNGG 6 cut(s) 725, 1019, 1329, 2583, 2811, 2841
SduI GDGCHC 5 cut(s) 55, 262, 1275, 1669, 1868
SexAI ACCWGGT 1 cut(s) 1327
SfcI CTRYAG 9 cut(s) 276, 285, 353, 594, 828, 2012, 2034, 2526, 2912
SfiI GGCCNNNNNGGCC 1 cut(s) 325
Sfr274I CTCGAG 1 cut(s) 1621
SfuI TTCGAA 1 cut(s) 1748
SinI GGWCC 2 cut(s) 181, 2573
SlaI CTCGAG 1 cut(s) 1621
SmiMI CAYNNNNRTG 2 cut(s) 267, 2097
SmlI CTYRAG 8 cut(s) 77, 176, 601, 1621, 1782, 2326, 2782, 2888
SmoI CTYRAG 8 cut(s) 77, 176, 601, 1621, 1782, 2326, 2782, 2888
SphI GCATGC 1 cut(s) 2360
SsiI CCGC 6 cut(s) 728, 750, 1237, 1268, 1980, 2998
SspI AATATT 1 cut(s) 1611
SspMI CTAG 3 cut(s) 1014, 2421, 2654
StyD4I CCNGG 6 cut(s) 723, 1017, 1327, 2581, 2809, 2839
StyI CCWWGG 2 cut(s) 1911, 2569
TaaI ACNGT 7 cut(s) 807, 852, 1404, 2050, 2503, 2774, 2982
TaiI ACGT 4 cut(s) 61, 1225, 1994, 2598
TaqI TCGA 9 cut(s) 210, 334, 709, 1463, 1478, 1622, 1748, 1816, 2554
TatI WGTACW 2 cut(s) 944, 1216
TauI GCSGC 2 cut(s) 1982, 3000
TscAI CASTG 7 cut(s) 274, 646, 1060, 2162, 2199, 2412, 2799
TseFI GTSAC 2 cut(s) 533, 2172
Tsp45I GTSAC 2 cut(s) 533, 2172
TspDTI ATGAA 7 cut(s) 27, 177, 1160, 1503, 1539, 1947, 2916
TspGWI ACGGA 2 cut(s) 974, 1296
TspRI CASTG 7 cut(s) 274, 646, 1060, 2162, 2199, 2412, 2799
Van91I CCANNNNNTGG 4 cut(s) 138, 641, 1425, 2411
Vha464I CTTAAG 2 cut(s) 2326, 2782
VpaK11BI GGWCC 2 cut(s) 181, 2573
XagI CCTNNNNNAGG 1 cut(s) 926
XapI RAATTY 5 cut(s) 117, 1099, 1421, 1760, 2257
XceI RCATGY 4 cut(s) 616, 1362, 2180, 2360
XcmI CCANNNNNNNNNTGG 1 cut(s) 2191
XhoI CTCGAG 1 cut(s) 1621
XmiI GTMKAC 3 cut(s) 1463, 2146, 2554
XmnI GAANNNNTTC 1 cut(s) 1387
XspI CTAG 3 cut(s) 1014, 2421, 2654
ZrmI AGTACT 1 cut(s) 946
Zsp2I ATGCAT 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.