Rmu_sc0009531.1_g000006

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009531.1
OG0008095
Physical Location & Seq
Forward (+)
27085 .. 32806
5722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009531.1_g000006.1.cds

Sequence Viewer

Length: 2814 bp
atgtctgcttctccatcatgggagcgtgcccaacgtttgttcgacaagattactgagttggagagtaggcgtcggaggtctgcccaagcacgagttccctcagatcctaatgcctggcaacagatacgtgagaattatgaagctataattcttgaggaccatgcattttcagagcagcacaatgttgagtatgctttgtggcagttacattacaagcggattgacgaattgcgcacgcacttcagtgctgctatggcttctacaggttcaaactcatcccaaggtttgaagggacctactcggcctgatcgtattacaaaaataagactgcagtttaaaacattcctttcagaagcgactggattttatcatgatttaatagtgaagatcagagcaaagtatgggcttcctcttggctatttctctgaggattctgaaaatcgaattgttctggataaggatggaaagaaatctgctgatatgaagaaaggtttgatatcatgccaccgctgtttgatttacttgggtgaccttgcacgctacaaaggactgtatggggagggggactccaaaactcgtgagtatgctgctgcctcaagttactacttgcaagctgcatcttgtttgccatcaagtgggaaccctcatcatcagcttgctatattggcttcttattctggggatgagttggtgactgtttatcgatattttcgtagcctggccgtggataatcccttttcaactgcaagggataacttgattgtggcatttgagaagaatcgtcagagttactcgcaactgtctggcaatgctaatgcttctgcactcaaagagttgcctgcacgattgactggcaaaggtagagggaagggggaagcaatacctgctaaagataacaacacagaagcaggccttgttaaggaaagatcatccagtaatcaggaaaaatacaaagctttttcagttcgttttgtccgtctaaatggcattctttttacacgtactagcctggagacatttgcagaagttctctctgttgtaagcagtggtttatatgagcttctatcttccggagcagaagaggagctgagtcttggtgctgatgctgttgagaatggactggtcattgttcgactggtctctatactgatattcacagttcataatgtgaaaaaggaaagtgaaggtcagtcatatgcagaaattgtacagcgtgctgttctgctgcaaaatgcatttactgctgtcttcgagttgatggggcatgtcttagagagatgcgtgaagctgggtgatccaacttcaagttatctgttaccaggcattctggtttttgtagaatggttggcatgttgtcctgatgtagcagctggcagtgatgcagatgaaaaacagtcttctgttagagcaaagttctggaatgcttgcatacacttgttgaataatctcttatcatctgttccgatgtccatagatgatgatggagatgagacttgctttaataatatgagcagatatgaagaaggggaaactgagaatcgccttgctttgtgggaggattttgagttgagaggattcatgccacttcttccagcacagacaatattggatttctcgaggaagcattcttttggaagtgatggccagaaggaaaaaggtgcgcgtgttaaaaggatcctggcggcaggcaaggctttagcaaatgtggtcaaggttgatcaaaacgccatttattttgattcccggacaaagaaatttgtcatcggtgttgagccttcaatgaatggcgattatatgcctacctcctttttaggcttggctaattccaatgataatctggaagaaaatcaagtggaggacaccaaaaatctgggaggtccatttcaaaagccagagttgaccatggatggggatgaggaagatgaagtaatagtttttaaaccaatagtggctgagaagaggcctgatgtggtaggcactacatgggctatcccccagcatttggaaccatttaaatcagaagaaatatctcttgctaacaacctaaagagtttgggatttatgggcaatgggcaagtactgaaatctgaccatgtatcaagttcggttccattccaacagcctgttaatggtagtactagtagtatgttttacagccatgcaaaggctccagaagctgtgttgccgtttaaggttggtactattgcatcttctggaccatttgcagatggtttgacattgaagacgtcatcagctttgcccgctgctatccgaaaaaatccagttagtcgacctgttagacaccttggtccgccacctggttttagccatgttcccactaaacaagtgaacgagtctatttataattcagagtcaatgggtgagaatccactgatggatgattacagctggttggatgggtatcaggtgccatcttcaactaaaggaaatgcattcagcagttccattaactactcatctcattcaaatcttcatcgcgttcctaatgggaatggcttaagtggaacgcagaaaaccagaaaagcctgcaagaattccatatgctcgatgatcttaaacttcaccatgaaatgcagctgcagcagcagcaacaacctgagcaatatcaaggacagtctgtctggacaggccgctattttgtgtgatgccagtatcaatgtgatggttcttgaggatgatttgcattggtggtttggcttcttgctggcgcttacaaatacccaggttttgccaagctctccagcccgggggatgacaaatatggatatatgtttcatggtgcgaaattcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

937

Amino Acids

103.39

Weight (kDa)

6.33

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2364
AasI GACNNNNNNGTC 1 cut(s) 2307
AatII GACGTC 1 cut(s) 2249
Acc16I TGCGCA 1 cut(s) 233
Acc36I ACCTGC 1 cut(s) 892
AccB1I GGYRCC 1 cut(s) 2428
AccB7I CCANNNNNTGG 2 cut(s) 635, 2002
AccI GTMKAC 1 cut(s) 2290
AccII CGCG 2 cut(s) 1663, 2499
AccIII TCCGGA 1 cut(s) 1070
AciI CCGC 6 cut(s) 217, 508, 1682, 2262, 2312, 2652
AclI AACGTT 1 cut(s) 34
AclWI GGATC 4 cut(s) 98, 1289, 1669, 1682
AcoI YGGCCR 2 cut(s) 720, 1642
AcsI RAATTY 3 cut(s) 1754, 2554, 2806
AcuI CTGAAG 1 cut(s) 226
AcyI GRCGYC 2 cut(s) 70, 2246
AfaI GTAC 5 cut(s) 1003, 1209, 2079, 2137, 2200
AfiI CCNNNNNNNGG 9 cut(s) 635, 724, 919, 1908, 2002, 2129, 2164, 2318, 2768
AflII CTTAAG 1 cut(s) 2518
AflIII ACRYGT 1 cut(s) 998
AhdI GACNNNNNGTC 1 cut(s) 2638
AhlI ACTAGT 1 cut(s) 2138
AjnI CCWGG 7 cut(s) 113, 717, 1008, 1318, 1677, 2317, 2742
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AleI CACNNNNGTG 1 cut(s) 243
Alw26I GTCTC 3 cut(s) 1007, 1144, 1484
AlwI GGATC 4 cut(s) 98, 1289, 1669, 1682
AlwNI CAGNNNCTG 1 cut(s) 2177
Ama87I CYCGRG 2 cut(s) 1615, 2766
Aor13HI TCCGGA 1 cut(s) 1070
AoxI GGCC 6 cut(s) 302, 720, 910, 1642, 1961, 2649
ApoI RAATTY 3 cut(s) 1754, 2554, 2806
AspLEI GCGC 3 cut(s) 234, 1663, 2731
AspS9I GGNCC 5 cut(s) 157, 293, 1877, 2216, 2309
AsuC2I CCSGG 3 cut(s) 1744, 2767, 2768
AsuHPI GGTGA 5 cut(s) 539, 703, 1304, 2393, 2575
AvaI CYCGRG 2 cut(s) 1615, 2766
AvaII GGWCC 5 cut(s) 157, 293, 1877, 2216, 2309
BaeGI GKGCMC 1 cut(s) 31
BalI TGGCCA 1 cut(s) 1644
BamHI GGATCC 1 cut(s) 1674
BanI GGYRCC 1 cut(s) 2428
BauI CACGAG 2 cut(s) 90, 576
BbsI GAAGAC 3 cut(s) 1240, 1389, 2249
BceAI ACGGC 2 cut(s) 707, 2170
BciT130I CCWGG 7 cut(s) 115, 719, 1010, 1320, 1679, 2319, 2744
BclI TGATCA 1 cut(s) 1717
BcnI CCSGG 3 cut(s) 1744, 2767, 2768
BcoDI GTCTC 3 cut(s) 1007, 1144, 1484
BcuI ACTAGT 1 cut(s) 2138
BfaI CTAG 2 cut(s) 1005, 2139
BfmI CTRYAG 3 cut(s) 261, 329, 2599
BfoI RGCGCY 1 cut(s) 2732
BfrI CTTAAG 1 cut(s) 2518
BfuAI ACCTGC 1 cut(s) 892
BmcAI AGTACT 2 cut(s) 2079, 2137
Bme18I GGWCC 5 cut(s) 157, 293, 1877, 2216, 2309
BmeRI GACNNNNNGTC 1 cut(s) 2638
BmeT110I CYCGRG 2 cut(s) 1615, 2766
BmgT120I GGNCC 5 cut(s) 157, 293, 1877, 2216, 2309
BmiI GGNNCC 7 cut(s) 294, 641, 1676, 2007, 2109, 2169, 2430
BmsI GCATC 6 cut(s) 626, 1093, 1268, 1369, 2216, 2656
BpiI GAAGAC 3 cut(s) 1240, 1389, 2249
BplI GAGNNNNNCTC 2 cut(s) 551, 583
BpmI CTGGAG 3 cut(s) 1031, 2154, 2745
Bpu10I CCTNAGC 1 cut(s) 2618
BpuEI CTTGAG 3 cut(s) 173, 580, 2711
BpuMI CCSGG 3 cut(s) 1744, 2767, 2768
Bsa29I ATCGAT 1 cut(s) 703
BsaAI YACGTR 2 cut(s) 128, 1001
BsaBI GATNNNNATC 1 cut(s) 2421
BsaHI GRCGYC 2 cut(s) 70, 2246
BsaI GGTCTC 1 cut(s) 1144
BsaJI CCNNGG 7 cut(s) 280, 723, 1902, 2305, 2742, 2766, 2767
BsaWI WCCGGW 1 cut(s) 1070
Bsc4I CCNNNNNNNGG 9 cut(s) 635, 724, 919, 1908, 2002, 2129, 2164, 2318, 2768
Bse1I ACTGG 7 cut(s) 364, 856, 933, 1125, 1140, 2282, 2670
Bse3DI GCAATG 2 cut(s) 814, 2074
Bse8I GATNNNNATC 1 cut(s) 2421
BseAI TCCGGA 1 cut(s) 1070
BseBI CCWGG 7 cut(s) 115, 719, 1010, 1320, 1679, 2319, 2744
BseCI ATCGAT 1 cut(s) 703
BseDI CCNNGG 7 cut(s) 280, 723, 1902, 2305, 2742, 2766, 2767
BseJI GATNNNNATC 1 cut(s) 2421
BseLI CCNNNNNNNGG 9 cut(s) 635, 724, 919, 1908, 2002, 2129, 2164, 2318, 2768
BseMI GCAATG 2 cut(s) 814, 2074
BseMII CTCAG 7 cut(s) 45, 114, 417, 1079, 1524, 1944, 2609
BseNI ACTGG 7 cut(s) 364, 856, 933, 1125, 1140, 2282, 2670
BseRI GAGGAG 1 cut(s) 1097
BseSI GKGCMC 1 cut(s) 31
BseYI CCCAGC 2 cut(s) 1288, 1995
BsgI GTGCAG 2 cut(s) 807, 825
Bsh1236I CGCG 2 cut(s) 1663, 2499
BshFI GGCC 6 cut(s) 304, 722, 912, 1644, 1963, 2651
BshNI GGYRCC 1 cut(s) 2428
BshVI ATCGAT 1 cut(s) 703
BsiHKCI CYCGRG 2 cut(s) 1615, 2766
BsiSI CCGG 3 cut(s) 1071, 1744, 2767
BslFI GGGAC 2 cut(s) 306, 578
BslI CCNNNNNNNGG 9 cut(s) 635, 724, 919, 1908, 2002, 2129, 2164, 2318, 2768
BsmAI GTCTC 3 cut(s) 1007, 1144, 1484
BsmFI GGGAC 2 cut(s) 306, 578
BsmI GAATGC 5 cut(s) 987, 1323, 1426, 1624, 2453
BsnI GGCC 6 cut(s) 304, 722, 912, 1644, 1963, 2651
Bso31I GGTCTC 1 cut(s) 1144
BsoBI CYCGRG 2 cut(s) 1615, 2766
Bsp1286I GDGCHC 1 cut(s) 31
Bsp13I TCCGGA 1 cut(s) 1070
Bsp1407I TGTACA 1 cut(s) 1207
Bsp143I GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2571
Bsp19I CCATGG 1 cut(s) 1902
BspACI CCGC 6 cut(s) 217, 508, 1682, 2262, 2312, 2652
BspANI GGCC 6 cut(s) 304, 722, 912, 1644, 1963, 2651
BspCNI CTCAG 7 cut(s) 46, 113, 418, 1080, 1525, 1945, 2610
BspDI ATCGAT 1 cut(s) 703
BspEI TCCGGA 1 cut(s) 1070
BspFNI CGCG 2 cut(s) 1663, 2499
BspHI TCATGA 1 cut(s) 370
BspLI GGNNCC 7 cut(s) 294, 641, 1676, 2007, 2109, 2169, 2430
BspMAI CTGCAG 2 cut(s) 333, 2603
BspMI ACCTGC 1 cut(s) 892
BspPI GGATC 4 cut(s) 98, 1289, 1669, 1682
BspT107I GGYRCC 1 cut(s) 2428
BspTI CTTAAG 1 cut(s) 2518
BspTNI GGTCTC 1 cut(s) 1144
BsrDI GCAATG 2 cut(s) 814, 2074
BsrGI TGTACA 1 cut(s) 1207
BsrI ACTGG 7 cut(s) 364, 856, 933, 1125, 1140, 2282, 2670
BssECI CCNNGG 7 cut(s) 280, 723, 1902, 2305, 2742, 2766, 2767
BssMI GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2571
BssNI GRCGYC 2 cut(s) 70, 2246
BssSI CACGAG 2 cut(s) 90, 576
BssT1I CCWWGG 3 cut(s) 280, 1902, 2305
Bst2BI CACGAG 2 cut(s) 90, 576
Bst2UI CCWGG 7 cut(s) 115, 719, 1010, 1320, 1679, 2319, 2744
Bst4CI ACNGT 6 cut(s) 552, 697, 801, 1159, 1395, 2636
Bst6I CTCTTC 2 cut(s) 1074, 1952
BstACI GRCGYC 2 cut(s) 70, 2246
BstAFI CTTAAG 1 cut(s) 2518
BstAPI GCANNNNNTGC 2 cut(s) 884, 1241
BstAUI TGTACA 1 cut(s) 1207
BstBAI YACGTR 2 cut(s) 128, 1001
BstDEI CTNAG 8 cut(s) 54, 100, 426, 1088, 1270, 1533, 1953, 2618
BstDSI CCRYGG 2 cut(s) 723, 1902
BstEII GGTNACC 1 cut(s) 527
BstENI CCTNNNNNAGG 1 cut(s) 917
BstFNI CGCG 2 cut(s) 1663, 2499
BstH2I RGCGCY 1 cut(s) 2732
BstHHI GCGC 3 cut(s) 234, 1663, 2731
BstKTI GATC 8 cut(s) 106, 310, 390, 929, 1297, 1677, 1720, 2574
BstMAI GTCTC 3 cut(s) 1007, 1144, 1484
BstMBI GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2571
BstNI CCWGG 7 cut(s) 115, 719, 1010, 1320, 1679, 2319, 2744
BstNSI RCATGY 2 cut(s) 1268, 1353
BstPI GGTNACC 1 cut(s) 527
BstSFI CTRYAG 3 cut(s) 261, 329, 2599
BstSLI GKGCMC 1 cut(s) 31
BstUI CGCG 2 cut(s) 1663, 2499
BstV2I GAAGAC 3 cut(s) 1240, 1389, 2249
BstX2I RGATCY 2 cut(s) 103, 1674
BstXI CCANNNNNNTGG 1 cut(s) 1870
BstYI RGATCY 2 cut(s) 103, 1674
Bsu15I ATCGAT 1 cut(s) 703
BsuRI GGCC 6 cut(s) 304, 722, 912, 1644, 1963, 2651
BsuTUI ATCGAT 1 cut(s) 703
BtgI CCRYGG 2 cut(s) 723, 1902
BtgZI GCGATG 1 cut(s) 2480
BtsI GCAGTG 2 cut(s) 1051, 1381
BtsIMutI CAGTG 4 cut(s) 250, 1051, 1381, 2390
BveI ACCTGC 1 cut(s) 892
CaiI CAGNNNCTG 1 cut(s) 2177
CciI TCATGA 1 cut(s) 370
CfoI GCGC 3 cut(s) 234, 1663, 2731
Cfr13I GGNCC 5 cut(s) 157, 293, 1877, 2216, 2309
Cfr9I CCCGGG 1 cut(s) 2766
ClaI ATCGAT 1 cut(s) 703
CseI GACGC 1 cut(s) 59
CsiI ACCWGGT 1 cut(s) 2317
Csp6I GTAC 5 cut(s) 1002, 1208, 2078, 2136, 2199
CviQI GTAC 5 cut(s) 1002, 1208, 2078, 2136, 2199
DdeI CTNAG 8 cut(s) 54, 100, 426, 1088, 1270, 1533, 1953, 2618
DpnI GATC 8 cut(s) 105, 309, 389, 928, 1296, 1676, 1719, 2573
DpnII GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2571
DraI TTTAAA 3 cut(s) 337, 1939, 2014
DrdI GACNNNNNNGTC 1 cut(s) 2307
DriI GACNNNNNGTC 1 cut(s) 2638
DseDI GACNNNNNNGTC 1 cut(s) 2307
EaeI YGGCCR 2 cut(s) 720, 1642
Eam1104I CTCTTC 2 cut(s) 1074, 1952
Eam1105I GACNNNNNGTC 1 cut(s) 2638
EarI CTCTTC 2 cut(s) 1074, 1952
EciI GGCGGA 1 cut(s) 2301
Eco130I CCWWGG 3 cut(s) 280, 1902, 2305
Eco147I AGGCCT 2 cut(s) 912, 1963
Eco31I GGTCTC 1 cut(s) 1144
Eco32I GATATC 1 cut(s) 498
Eco47I GGWCC 5 cut(s) 157, 293, 1877, 2216, 2309
Eco57I CTGAAG 1 cut(s) 226
Eco88I CYCGRG 2 cut(s) 1615, 2766
Eco91I GGTNACC 1 cut(s) 527
EcoNI CCTNNNNNAGG 1 cut(s) 917
EcoO109I RGGNCCY 1 cut(s) 293
EcoO65I GGTNACC 1 cut(s) 527
EcoRI GAATTC 1 cut(s) 2554
EcoRII CCWGG 7 cut(s) 113, 717, 1008, 1318, 1677, 2317, 2742
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 3 cut(s) 280, 1902, 2305
EcoT22I ATGCAT 3 cut(s) 166, 1237, 2455
ErhI CCWWGG 3 cut(s) 280, 1902, 2305
FalI AAGNNNNNCTT 2 cut(s) 897, 929
FaqI GGGAC 2 cut(s) 306, 578
FauI CCCGC 1 cut(s) 2269
FauNDI CATATG 2 cut(s) 1195, 2561
FbaI TGATCA 1 cut(s) 1717
FblI GTMKAC 1 cut(s) 2290
FspBI CTAG 2 cut(s) 1005, 2139
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 3 cut(s) 233, 1662, 2730
GsaI CCCAGC 2 cut(s) 1292, 1999
GsuI CTGGAG 3 cut(s) 1031, 2154, 2745
HaeII RGCGCY 1 cut(s) 2732
HaeIII GGCC 6 cut(s) 304, 722, 912, 1644, 1963, 2651
HapII CCGG 3 cut(s) 1071, 1744, 2767
HgaI GACGC 1 cut(s) 59
HhaI GCGC 3 cut(s) 234, 1663, 2731
Hin1I GRCGYC 2 cut(s) 70, 2246
Hin6I GCGC 3 cut(s) 232, 1661, 2729
HinP1I GCGC 3 cut(s) 232, 1661, 2729
HincII GTYRAC 2 cut(s) 1899, 2291
HindII GTYRAC 2 cut(s) 1899, 2291
HindIII AAGCTT 1 cut(s) 954
HpaII CCGG 3 cut(s) 1071, 1744, 2767
HphI GGTGA 5 cut(s) 539, 703, 1304, 2393, 2575
Hpy166II GTNNAC 3 cut(s) 1899, 2291, 2350
Hpy8I GTNNAC 3 cut(s) 1899, 2291, 2350
Hpy99I CGWCG 1 cut(s) 75
HpyAV CCTTC 6 cut(s) 283, 862, 1178, 1517, 1642, 1785
HpyCH4III ACNGT 6 cut(s) 552, 697, 801, 1159, 1395, 2636
HpyCH4IV ACGT 4 cut(s) 34, 127, 1000, 2246
HpyF3I CTNAG 8 cut(s) 54, 100, 426, 1088, 1270, 1533, 1953, 2618
HpySE526I ACGT 4 cut(s) 34, 127, 1000, 2246
Hsp92I GRCGYC 2 cut(s) 70, 2246
HspAI GCGC 3 cut(s) 232, 1661, 2729
Kpn2I TCCGGA 1 cut(s) 1070
Ksp22I TGATCA 1 cut(s) 1717
Kzo9I GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2571
LmnI GCTCC 4 cut(s) 22, 1073, 1084, 2173
LweI GCATC 6 cut(s) 626, 1093, 1268, 1369, 2216, 2656
MabI ACCWGGT 1 cut(s) 2317
MaeI CTAG 2 cut(s) 1005, 2139
MaeII ACGT 4 cut(s) 34, 127, 1000, 2246
MaeIII GTNAC 6 cut(s) 204, 527, 599, 691, 788, 1314
MalI GATC 8 cut(s) 105, 309, 389, 928, 1296, 1676, 1719, 2573
MboI GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2571
MflI RGATCY 2 cut(s) 103, 1674
MhlI GDGCHC 1 cut(s) 31
MlsI TGGCCA 1 cut(s) 1644
MluNI TGGCCA 1 cut(s) 1644
MlyI GAGTC 4 cut(s) 560, 1099, 2363, 2381
MmeI TCCRAC 5 cut(s) 39, 53, 1322, 2140, 2394
Mox20I TGGCCA 1 cut(s) 1644
Mph1103I ATGCAT 3 cut(s) 166, 1237, 2455
MroI TCCGGA 1 cut(s) 1070
MscI TGGCCA 1 cut(s) 1644
MslI CAYNNNNRTG 1 cut(s) 243
Msp20I TGGCCA 1 cut(s) 1644
MspA1I CMGCKG 5 cut(s) 510, 1370, 2264, 2409, 2598
MspCI CTTAAG 1 cut(s) 2518
MspI CCGG 3 cut(s) 1071, 1744, 2767
Mva1269I GAATGC 5 cut(s) 987, 1323, 1426, 1624, 2453
MvaI CCWGG 7 cut(s) 115, 719, 1010, 1320, 1679, 2319, 2744
MvnI CGCG 2 cut(s) 1663, 2499
NciI CCSGG 3 cut(s) 1744, 2767, 2768
NcoI CCATGG 1 cut(s) 1902
NdeI CATATG 2 cut(s) 1195, 2561
NdeII GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2571
NlaIV GGNNCC 7 cut(s) 294, 641, 1676, 2007, 2109, 2169, 2430
NmeAIII GCCGAG 1 cut(s) 280
NmuCI GTSAC 2 cut(s) 527, 691
NsbI TGCGCA 1 cut(s) 233
NsiI ATGCAT 3 cut(s) 166, 1237, 2455
NspI RCATGY 2 cut(s) 1268, 1353
OliI CACNNNNGTG 1 cut(s) 243
PaeR7I CTCGAG 1 cut(s) 1615
PagI TCATGA 1 cut(s) 370
PceI AGGCCT 2 cut(s) 912, 1963
PcsI WCGNNNNNNNCGW 3 cut(s) 307, 709, 973
PctI GAATGC 5 cut(s) 987, 1323, 1426, 1624, 2453
PfeI GAWTC 6 cut(s) 431, 778, 1537, 1575, 1739, 2386
PflMI CCANNNNNTGG 2 cut(s) 635, 2002
PfoI TCCNGGA 1 cut(s) 1742
PleI GAGTC 4 cut(s) 560, 1098, 2362, 2380
PpsI GAGTC 4 cut(s) 560, 1098, 2362, 2380
Ppu21I YACGTR 2 cut(s) 128, 1001
PpuMI RGGWCCY 1 cut(s) 293
PsiI TTATAA 1 cut(s) 2364
Psp1406I AACGTT 1 cut(s) 34
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 7 cut(s) 113, 717, 1008, 1318, 1677, 2317, 2742
PspEI GGTNACC 1 cut(s) 527
PspFI CCCAGC 2 cut(s) 1288, 1995
PspGI CCWGG 7 cut(s) 113, 717, 1008, 1318, 1677, 2317, 2742
PspN4I GGNNCC 7 cut(s) 294, 641, 1676, 2007, 2109, 2169, 2430
PspPI GGNCC 5 cut(s) 157, 293, 1877, 2216, 2309
PspPPI RGGWCCY 1 cut(s) 293
PstI CTGCAG 2 cut(s) 333, 2603
PstNI CAGNNNCTG 1 cut(s) 2177
PsuI RGATCY 2 cut(s) 103, 1674
PvuII CAGCTG 3 cut(s) 1370, 2409, 2598
RsaI GTAC 5 cut(s) 1003, 1209, 2079, 2137, 2200
RsaNI GTAC 5 cut(s) 1002, 1208, 2078, 2136, 2199
RseI CAYNNNNRTG 1 cut(s) 243
SalI GTCGAC 1 cut(s) 2289
Sau3AI GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2571
Sau96I GGNCC 5 cut(s) 157, 293, 1877, 2216, 2309
ScaI AGTACT 2 cut(s) 2079, 2137
SchI GAGTC 4 cut(s) 560, 1099, 2363, 2381
SduI GDGCHC 1 cut(s) 31
SexAI ACCWGGT 1 cut(s) 2317
SfaNI GCATC 6 cut(s) 626, 1093, 1268, 1369, 2216, 2656
SfcI CTRYAG 3 cut(s) 261, 329, 2599
Sfr274I CTCGAG 1 cut(s) 1615
SinI GGWCC 5 cut(s) 157, 293, 1877, 2216, 2309
SlaI CTCGAG 1 cut(s) 1615
SmaI CCCGGG 1 cut(s) 2768
SmiI ATTTAAAT 1 cut(s) 2014
SmiMI CAYNNNNRTG 1 cut(s) 243
SmlI CTYRAG 5 cut(s) 152, 595, 1615, 2518, 2690
SmoI CTYRAG 5 cut(s) 152, 595, 1615, 2518, 2690
SpeI ACTAGT 1 cut(s) 2138
SseBI AGGCCT 2 cut(s) 912, 1963
SsiI CCGC 6 cut(s) 217, 508, 1682, 2262, 2312, 2652
SspI AATATT 1 cut(s) 1605
SspMI CTAG 2 cut(s) 1005, 2139
StuI AGGCCT 2 cut(s) 912, 1963
StyI CCWWGG 3 cut(s) 280, 1902, 2305
SwaI ATTTAAAT 1 cut(s) 2014
TaaI ACNGT 6 cut(s) 552, 697, 801, 1159, 1395, 2636
TaiI ACGT 4 cut(s) 37, 130, 1003, 2249
TaqI TCGA 8 cut(s) 42, 442, 703, 1132, 1251, 1616, 2290, 2567
TatI WGTACW 3 cut(s) 1207, 2077, 2135
TauI GCSGC 2 cut(s) 1685, 2654
TfiI GAWTC 6 cut(s) 431, 778, 1537, 1575, 1739, 2386
TscAI CASTG 4 cut(s) 250, 1051, 1381, 2397
TseFI GTSAC 2 cut(s) 527, 691
Tsp45I GTSAC 2 cut(s) 527, 691
TspGWI ACGGA 1 cut(s) 965
TspMI CCCGGG 1 cut(s) 2766
TspRI CASTG 4 cut(s) 250, 1051, 1381, 2397
Van91I CCANNNNNTGG 2 cut(s) 635, 2002
Vha464I CTTAAG 1 cut(s) 2518
VpaK11BI GGWCC 5 cut(s) 157, 293, 1877, 2216, 2309
XagI CCTNNNNNAGG 1 cut(s) 917
XapI RAATTY 3 cut(s) 1754, 2554, 2806
XceI RCATGY 2 cut(s) 1268, 1353
XcmI CCANNNNNNNNNTGG 1 cut(s) 1834
XhoI CTCGAG 1 cut(s) 1615
XmaI CCCGGG 1 cut(s) 2766
XmiI GTMKAC 1 cut(s) 2290
XspI CTAG 2 cut(s) 1005, 2139
ZraI GACGTC 1 cut(s) 2247
ZrmI AGTACT 2 cut(s) 2079, 2137
Zsp2I ATGCAT 3 cut(s) 166, 1237, 2455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.