MD03G1165600.v1.1

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008095
Physical Location & Seq
Forward (+)
21970849 .. 21977892
7044 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1165600.v1.1.491

Sequence Viewer

Length: 3021 bp
ATGATTCCACAGATGGATAACATGTCTGCTCCTTCATCAAGGGAGCGTGCCCAACGCCTGTATGACAAGATTCTTGAGTTGGAGAATAGGCGTCGAAGGTCTGCCCAAGCACGAATTCCATCAGATCCCAATGCCTGGCAACAGATGCGTGAGAATTATGAAGCAATAATTCTTGAGGATCATGCTTTTTCTGAGCAGAACAATATCGAATATGCTTTGTGGCAGTTACATTACAAGCGTATTGAGGAATTGCGAGCACACTTCAGTGCTGCTATGGCTTCTACAGGTTCAAACACATCTCAGGGTGTGAAAGGGCCAATTAGGCCTGATCGGGTTACAAAAATAAGACTGCAGTTTAAAACATTCCTTTCAGAAGCAACTGGATTTTATCATGATTTAATAGTGAAGATCAGAGCAAAGTATGGGCTTCCTCTGGGTTATTTCTCTGAAGATTCTGAAAACCGGACCGTTATAGACAAGGATGGAAAGAAAGGTCTAATTTCATGCCACAGATGCTTGATATATTTGGGTGACCTTGCACGCTACAAAGGACTGTATGGGGAAGGGGACTCAAAAACTCGTGAGTATGCTGCAGCCTCAAGTTACTATATGCAAGCTGCATCTCTCTGGCCATCCAGTGGGAACCCCCATCATCAGCTTGCTATATTGGCTTCCTATTCGGGGGATGAGTTGGTGGCTGTTTATCGATATTTTCGTAGCCTGGCGGTGGATTGTCCCTTTTCAACTGCGAGAGATAACTTGATTGTTGCATTTGAGAAGAATCGTCAGAGTTACTCTCAACCGTCTGGGAATACTAATGCTTCTGCAGTCAAAGAGTTGCCTGCACGGTTGGCAGGCAAAGGTAGAGGGAAAGGGGAAACAATACCCACATCTAAAGATAACAGTACTGAAGTGAGCCTGGTTAAGGAAAGAGCATCCAGTACCCAGGAGACTTATAAGGCTTTTTGCATCCGTTTTGTCCGTCTGAATGGCATTCTTTTCACTCGTACTAGCCTGGAGACATTTGCAGAAGTTCTAACTGTGGTAAGCAGTGGCTTATGTGAGCTTCTGTCTTCTGGAGGAGAAGAGGTGCTGAATTTTGGTGCAGATGCTGTTGAAAATGGACTTGTTATTGTTAGACTTATCTCCATATTGATATTCACAGTTCATATTGTGAAAAAGGAAAGCGAAGGTCAGACATATGCAGAAATTGTACAACGTGCTGTCCTGCTGCAGAATGCATTTACTGCTGTTTTTGAGTTGATGGGACACATCTTAGAGAGATGTGTGCAGTTGTGTGATCCTTCTTCAAGTTTCCTGTTACCAGGTATTTTGGTTTTTGTTGAATGGTTGGCATGCTGTCCTGATGTTGCAGCTGGAAGTGATGCAGATGAAAAACAGTCGTCTGTTAGATCCAAATTCTGGAATGTATGTATATCCTTCTTCAATAGTCTCTCGTCTACTGGGCCAATGTCGATAGATGATGATGAAGATGAGACTTGCTTTAATAACATGAGCAGATACGAAGAAGGTGAAACTGAAAACCGTCTTGCTCTGTGGGAGGATTTTGAGTTGAGAGGATTTACTCCACTAATTCCAGCACAAACAATATTGGACTTCTCGAGGAAGCATTCTTTTGGAAGTGATGGCCATAAGGAAAAAGGGGCCCGTGTTAAGAGGATTCTGGCAGCAGGCAAAGCTCTTGCAAATGTGGTTAAGGTTGACCAGGAAGCAGTATATTTTGATTCGAAGTTAAAGAAATTTGTCATCGGTTTTGAGCCTCAAGTGCAAAATGATATTGTTCCTACCACCTATCTACATATGGCTAATGGAAATGATAATCTGCAAGAAAATCAAGTAGTGGGCACAATCAGTTTGGGAGCTGCATATCCAAAGCCTGAGTTATCCATGGAAGGGGATGAGGAAGATGAAGTAATAGTCTTTAAACCAATAGTGGCTGAGAAGCGACCTGATGCAGTAAGCACCGCATGGAGAGCCTATGATGGCCTAGAGCCTGGTAAAAATGCTTCTCCAGGTGATCAAACAGTTAATGTAACCTATGGTTCTGCCGCTTTGGATAATCTACACCATCAAAATGCCTTTAGGGCTGATTTACAAATACCTGTAACTTTTGCAAATGGTATACCCCAACACTTAAAACCAATCCAGTCACATGGTTCCAAGTTTTCTGTGGATACTGGTTTTGGTGTTGGTTCACAACTACCTGCATCAATTACCAATACAGTACCCCTGAATTTGCAACCAGTTCAATCAAATTCCTTGAATCTTGCAATGGAGGAAGAAATGTCTCTTGCTAATAGCTTAAAGAGTATGGGATTTATGGGGAATGGGCATGCACTAAAATCTGAGCCTGTAGCAGTTTCTGTTCCATACCAACACCCAGTGAATGGTAGTACTAGTGGTATGTTTTACAGCCACACAAAAGCTCCAGAAAATCCTTTGTCTTATAAAGTTGATGCAACTTATGGAGCAATCGCTGACGGGTTGACTGTAAAGACATCATCAGCTCTGCCTACAGGTATCCGAAAAAGTCCAGTAAGTCGACCTGTTAGGCACCTTGGTCCACCACCCGGTTTTAGCCGTGTTCCTCCCAAAAATGCGAATGAATCAATTTGTAGTTCAGATTCAATGAGTGAGAGTCTAGTGATGGATGATTACAGCTGGTTGGATGGATATCAGATGCCATCTTCAACAAAAGTAAATGGGCTCAATGATTATGTTAGTTATTCATCTCAATACAATCCCCATCGCTTCACAAACAGCAACGGCTTAAGTGGAACAGTGAACTTCCCCTTTCCTGGTAAACAGGGTCCACCCATGCAACTACCAGGGGAAAACCAGAAAAGCTGGCAAGATATCCAGAGGCTTGATGACCTCAAGCTACACCATGAAATGCAGCTGCAGCAACAGCAACAACTTGTAAATGGAAATCAGCACTTGAATCCACAGCCTGAGCAATATCAAGGACAGTCTCTCTGGACGGGCCGCCATTTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

1007

Amino Acids

111.38

Weight (kDa)

5.97

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 69 - 186 1.3e-16 Telomerase activating protein Est1
EST1_DNA_bind PF10373 199 - 533 7.6e-64 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 957, 2469
Acc36I ACCTGC 1 cut(s) 2233
AccB1I GGYRCC 1 cut(s) 2574
AccB7I CCANNNNNTGG 4 cut(s) 135, 638, 1422, 2408
AccI GTMKAC 3 cut(s) 1460, 2143, 2563
AciI CCGC 4 cut(s) 725, 1986, 2070, 3007
AclWI GGATC 4 cut(s) 119, 186, 1295, 1407
AcoI YGGCCR 2 cut(s) 629, 1648
AcsI RAATTY 6 cut(s) 114, 1096, 1418, 1760, 2254, 2275
AcuI CTGAAG 3 cut(s) 247, 468, 930
AcyI GRCGYC 1 cut(s) 91
AdeI CACNNNGTG 1 cut(s) 2404
AfaI GTAC 6 cut(s) 907, 943, 1009, 1215, 2247, 2416
AflII CTTAAG 1 cut(s) 2791
AflIII ACRYGT 1 cut(s) 21
AhlI ACTAGT 1 cut(s) 2417
AjuI GAANNNNNNNTTGG 2 cut(s) 99, 131
AleI CACNNNNGTG 1 cut(s) 264
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 6 cut(s) 944, 1013, 1457, 1490, 2313, 2997
AlwI GGATC 4 cut(s) 119, 186, 1295, 1407
AlwNI CAGNNNCTG 2 cut(s) 1112, 2384
Ama87I CYCGRG 1 cut(s) 1621
AoxI GGCC 8 cut(s) 314, 323, 629, 1466, 1648, 1665, 2005, 3004
ApaI GGGCCC 1 cut(s) 1669
ApoI RAATTY 6 cut(s) 114, 1096, 1418, 1760, 2254, 2275
AspS9I GGNCC 8 cut(s) 314, 465, 1466, 1665, 1666, 2582, 2831, 3004
AsuC2I CCSGG 1 cut(s) 2592
AsuHPI GGTGA 3 cut(s) 542, 1544, 2048
AsuII TTCGAA 1 cut(s) 1748
AvaI CYCGRG 1 cut(s) 1621
AvaII GGWCC 3 cut(s) 465, 2582, 2831
BaeGI GKGCMC 3 cut(s) 52, 1669, 1868
BalI TGGCCA 2 cut(s) 631, 1650
BanI GGYRCC 1 cut(s) 2574
BanII GRGCYC 2 cut(s) 1669, 2730
BarI GAAGNNNNNNTAC 2 cut(s) 2011, 2043
BauI CACGAG 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 1065
Bbv12I GWGCWC 1 cut(s) 259
BceAI ACGGC 2 cut(s) 2586, 2803
BciVI GTATCC 2 cut(s) 2188, 2552
BclI TGATCA 1 cut(s) 2038
BcnI CCSGG 1 cut(s) 2592
BcoDI GTCTC 6 cut(s) 944, 1013, 1457, 1490, 2313, 2997
BcuI ACTAGT 1 cut(s) 2417
BfaI CTAG 4 cut(s) 1011, 2009, 2418, 2663
BfmI CTRYAG 8 cut(s) 282, 350, 591, 825, 1232, 2373, 2535, 2921
BfrI CTTAAG 1 cut(s) 2791
BfuAI ACCTGC 1 cut(s) 2233
BfuI GTATCC 2 cut(s) 2188, 2552
BglI GCCNNNNNGGC 2 cut(s) 322, 2105
BmcAI AGTACT 2 cut(s) 907, 2416
Bme18I GGWCC 3 cut(s) 465, 2582, 2831
BmeT110I CYCGRG 1 cut(s) 1621
BmgT120I GGNCC 8 cut(s) 314, 465, 1466, 1665, 1666, 2582, 2831, 3004
BmiI GGNNCC 6 cut(s) 644, 1666, 1667, 2179, 2576, 2832
BmrI ACTGGG 2 cut(s) 1473, 2396
BmuI ACTGGG 2 cut(s) 1473, 2396
BpiI GAAGAC 1 cut(s) 1065
BplI GAGNNNNNCTC 2 cut(s) 781, 813
BpmI CTGGAG 4 cut(s) 1037, 1098, 2016, 2433
Bpu10I CCTNAGC 1 cut(s) 2973
Bpu14I TTCGAA 1 cut(s) 1748
BpuEI CTTGAG 5 cut(s) 95, 194, 583, 1767, 2882
BpuMI CCSGG 1 cut(s) 2592
Bsa29I ATCGAT 1 cut(s) 706
BsaBI GATNNNNATC 1 cut(s) 2694
BsaHI GRCGYC 1 cut(s) 91
BsaJI CCNNGG 4 cut(s) 945, 1908, 2578, 2849
BsaWI WCCGGW 1 cut(s) 462
BsaXI ACNNNNNCTCC 4 cut(s) 1074, 1104, 2431, 2461
Bse1I ACTGG 9 cut(s) 385, 636, 939, 1468, 2167, 2203, 2264, 2402, 2555
Bse3DI GCAATG 1 cut(s) 2298
Bse8I GATNNNNATC 1 cut(s) 2694
BseCI ATCGAT 1 cut(s) 706
BseDI CCNNGG 4 cut(s) 945, 1908, 2578, 2849
BseGI GGATG 8 cut(s) 487, 632, 691, 935, 969, 1924, 2677, 2695
BseJI GATNNNNATC 1 cut(s) 2694
BseMI GCAATG 1 cut(s) 2298
BseMII CTCAG 6 cut(s) 183, 314, 1890, 1950, 2358, 2964
BseNI ACTGG 9 cut(s) 385, 636, 939, 1468, 2167, 2203, 2264, 2402, 2555
BseRI GAGGAG 1 cut(s) 1095
BseSI GKGCMC 3 cut(s) 52, 1669, 1868
BsgI GTGCAG 3 cut(s) 828, 1125, 1310
BshFI GGCC 8 cut(s) 316, 325, 631, 1468, 1650, 1667, 2007, 3006
BshNI GGYRCC 1 cut(s) 2574
BshVI ATCGAT 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 259
BsiHKCI CYCGRG 1 cut(s) 1621
BsiSI CCGG 2 cut(s) 463, 2592
BslFI GGGAC 3 cut(s) 581, 720, 1281
BsmAI GTCTC 6 cut(s) 944, 1013, 1457, 1490, 2313, 2997
BsmFI GGGAC 3 cut(s) 581, 720, 1281
BsmI GAATGC 3 cut(s) 993, 1243, 1630
BsnI GGCC 8 cut(s) 316, 325, 631, 1468, 1650, 1667, 2007, 3006
BsoBI CYCGRG 1 cut(s) 1621
Bsp119I TTCGAA 1 cut(s) 1748
Bsp120I GGGCCC 1 cut(s) 1665
Bsp1286I GDGCHC 5 cut(s) 52, 259, 1669, 1868, 2730
Bsp1407I TGTACA 1 cut(s) 1213
Bsp143I GATC 7 cut(s) 124, 178, 328, 408, 1300, 1412, 2038
Bsp19I CCATGG 1 cut(s) 1908
BspACI CCGC 4 cut(s) 725, 1986, 2070, 3007
BspANI GGCC 8 cut(s) 316, 325, 631, 1468, 1650, 1667, 2007, 3006
BspCNI CTCAG 6 cut(s) 184, 313, 1891, 1951, 2359, 2965
BspDI ATCGAT 1 cut(s) 706
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 6 cut(s) 644, 1666, 1667, 2179, 2576, 2832
BspMAI CTGCAG 5 cut(s) 354, 595, 829, 1236, 2925
BspMI ACCTGC 1 cut(s) 2233
BspPI GGATC 4 cut(s) 119, 186, 1295, 1407
BspT104I TTCGAA 1 cut(s) 1748
BspT107I GGYRCC 1 cut(s) 2574
BspTI CTTAAG 1 cut(s) 2791
BsrDI GCAATG 1 cut(s) 2298
BsrGI TGTACA 1 cut(s) 1213
BsrI ACTGG 9 cut(s) 385, 636, 939, 1468, 2167, 2203, 2264, 2402, 2555
BssECI CCNNGG 4 cut(s) 945, 1908, 2578, 2849
BssMI GATC 7 cut(s) 124, 178, 328, 408, 1300, 1412, 2038
BssNAI GTATAC 1 cut(s) 2144
BssNI GRCGYC 1 cut(s) 91
BssSI CACGAG 1 cut(s) 579
BssT1I CCWWGG 2 cut(s) 1908, 2578
Bst1107I GTATAC 1 cut(s) 2144
Bst2BI CACGAG 1 cut(s) 579
Bst6I CTCTTC 1 cut(s) 1080
BstACI GRCGYC 1 cut(s) 91
BstAFI CTTAAG 1 cut(s) 2791
BstAPI GCANNNNNTGC 2 cut(s) 145, 1247
BstAUI TGTACA 1 cut(s) 1213
BstBI TTCGAA 1 cut(s) 1748
BstDEI CTNAG 7 cut(s) 192, 300, 1276, 1899, 1959, 2367, 2973
BstDSI CCRYGG 1 cut(s) 1908
BstEII GGTNACC 1 cut(s) 530
BstENI CCTNNNNNAGG 1 cut(s) 923
BstF5I GGATG 8 cut(s) 487, 632, 691, 935, 969, 1924, 2677, 2695
BstKTI GATC 7 cut(s) 127, 181, 331, 411, 1303, 1415, 2041
BstMAI GTCTC 6 cut(s) 944, 1013, 1457, 1490, 2313, 2997
BstMBI GATC 7 cut(s) 124, 178, 328, 408, 1300, 1412, 2038
BstNSI RCATGY 3 cut(s) 25, 1359, 2357
BstPI GGTNACC 1 cut(s) 530
BstSFI CTRYAG 8 cut(s) 282, 350, 591, 825, 1232, 2373, 2535, 2921
BstSLI GKGCMC 3 cut(s) 52, 1669, 1868
BstV2I GAAGAC 1 cut(s) 1065
BstX2I RGATCY 2 cut(s) 124, 1412
BstXI CCANNNNNNTGG 1 cut(s) 2174
BstYI RGATCY 2 cut(s) 124, 1412
BstZ17I GTATAC 1 cut(s) 2144
Bsu15I ATCGAT 1 cut(s) 706
BsuI GTATCC 2 cut(s) 2188, 2552
BsuRI GGCC 8 cut(s) 316, 325, 631, 1468, 1650, 1667, 2007, 3006
BsuTUI ATCGAT 1 cut(s) 706
BtgI CCRYGG 1 cut(s) 1908
BtgZI GCGATG 1 cut(s) 2753
BtsCI GGATG 8 cut(s) 487, 632, 691, 935, 969, 1924, 2677, 2695
BtsI GCAGTG 1 cut(s) 1057
BtsIMutI CAGTG 5 cut(s) 271, 643, 1057, 2409, 2808
BveI ACCTGC 1 cut(s) 2233
CaiI CAGNNNCTG 2 cut(s) 1112, 2384
CciI TCATGA 1 cut(s) 391
Cfr13I GGNCC 8 cut(s) 314, 465, 1466, 1665, 1666, 2582, 2831, 3004
ClaI ATCGAT 1 cut(s) 706
CpoI CGGWCCG 1 cut(s) 465
CseI GACGC 1 cut(s) 80
CsiI ACCWGGT 1 cut(s) 1324
Csp6I GTAC 6 cut(s) 906, 942, 1008, 1214, 2246, 2415
CspI CGGWCCG 1 cut(s) 465
CviQI GTAC 6 cut(s) 906, 942, 1008, 1214, 2246, 2415
DdeI CTNAG 7 cut(s) 192, 300, 1276, 1899, 1959, 2367, 2973
DpnI GATC 7 cut(s) 126, 180, 330, 410, 1302, 1414, 2040
DpnII GATC 7 cut(s) 124, 178, 328, 408, 1300, 1412, 2038
DraI TTTAAA 2 cut(s) 358, 1945
DraIII CACNNNGTG 1 cut(s) 2404
EaeI YGGCCR 2 cut(s) 629, 1648
Eam1104I CTCTTC 1 cut(s) 1080
EarI CTCTTC 1 cut(s) 1080
Eco130I CCWWGG 2 cut(s) 1908, 2578
Eco147I AGGCCT 1 cut(s) 325
Eco24I GRGCYC 2 cut(s) 1669, 2730
Eco32I GATATC 2 cut(s) 2696, 2878
Eco47I GGWCC 3 cut(s) 465, 2582, 2831
Eco57I CTGAAG 3 cut(s) 247, 468, 930
Eco88I CYCGRG 1 cut(s) 1621
Eco91I GGTNACC 1 cut(s) 530
EcoNI CCTNNNNNAGG 1 cut(s) 923
EcoO109I RGGNCCY 1 cut(s) 1665
EcoO65I GGTNACC 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 114
EcoRV GATATC 2 cut(s) 2696, 2878
EcoT14I CCWWGG 2 cut(s) 1908, 2578
EcoT22I ATGCAT 1 cut(s) 1243
EcoT38I GRGCYC 2 cut(s) 1669, 2730
ErhI CCWWGG 2 cut(s) 1908, 2578
FaqI GGGAC 3 cut(s) 581, 720, 1281
FauNDI CATATG 2 cut(s) 1201, 1821
FbaI TGATCA 1 cut(s) 2038
FblI GTMKAC 3 cut(s) 1460, 2143, 2563
FokI GGATG 8 cut(s) 494, 619, 698, 922, 956, 1931, 2684, 2702
FriOI GRGCYC 2 cut(s) 1669, 2730
FspBI CTAG 4 cut(s) 1011, 2009, 2418, 2663
GsuI CTGGAG 4 cut(s) 1037, 1098, 2016, 2433
HaeIII GGCC 8 cut(s) 316, 325, 631, 1468, 1650, 1667, 2007, 3006
HapII CCGG 2 cut(s) 463, 2592
HgaI GACGC 1 cut(s) 80
Hin1I GRCGYC 1 cut(s) 91
HincII GTYRAC 3 cut(s) 1723, 2508, 2564
HindII GTYRAC 3 cut(s) 1723, 2508, 2564
HpaII CCGG 2 cut(s) 463, 2592
HphI GGTGA 3 cut(s) 542, 1544, 2048
Hpy99I CGWCG 1 cut(s) 96
HpyAV CCTTC 8 cut(s) 42, 90, 557, 1184, 1314, 1450, 1523, 1907
HpyCH4IV ACGT 1 cut(s) 1219
HpyF3I CTNAG 7 cut(s) 192, 300, 1276, 1899, 1959, 2367, 2973
HpySE526I ACGT 1 cut(s) 1219
Hsp92I GRCGYC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 2038
Kzo9I GATC 7 cut(s) 124, 178, 328, 408, 1300, 1412, 2038
LmnI GCTCC 5 cut(s) 34, 43, 1880, 2452, 2489
MabI ACCWGGT 1 cut(s) 1324
MaeI CTAG 4 cut(s) 1011, 2009, 2418, 2663
MaeII ACGT 1 cut(s) 1219
MaeIII GTNAC 9 cut(s) 225, 334, 530, 602, 791, 1320, 2053, 2125, 2169
MalI GATC 7 cut(s) 126, 180, 330, 410, 1302, 1414, 2040
MboI GATC 7 cut(s) 124, 178, 328, 408, 1300, 1412, 2038
MflI RGATCY 2 cut(s) 124, 1412
MhlI GDGCHC 5 cut(s) 52, 259, 1669, 1868, 2730
MlsI TGGCCA 2 cut(s) 631, 1650
MluNI TGGCCA 2 cut(s) 631, 1650
MlyI GAGTC 2 cut(s) 563, 2668
MmeI TCCRAC 2 cut(s) 60, 2667
Mox20I TGGCCA 2 cut(s) 631, 1650
Mph1103I ATGCAT 1 cut(s) 1243
MscI TGGCCA 2 cut(s) 631, 1650
MslI CAYNNNNRTG 2 cut(s) 264, 2094
Msp20I TGGCCA 2 cut(s) 631, 1650
MspA1I CMGCKG 3 cut(s) 1376, 2682, 2920
MspCI CTTAAG 1 cut(s) 2791
MspI CCGG 2 cut(s) 463, 2592
Mva1269I GAATGC 3 cut(s) 993, 1243, 1630
NciI CCSGG 1 cut(s) 2592
NcoI CCATGG 1 cut(s) 1908
NdeI CATATG 2 cut(s) 1201, 1821
NdeII GATC 7 cut(s) 124, 178, 328, 408, 1300, 1412, 2038
NlaIV GGNNCC 6 cut(s) 644, 1666, 1667, 2179, 2576, 2832
NmuCI GTSAC 2 cut(s) 530, 2169
NsiI ATGCAT 1 cut(s) 1243
NspI RCATGY 3 cut(s) 25, 1359, 2357
NspV TTCGAA 1 cut(s) 1748
OliI CACNNNNGTG 1 cut(s) 264
PaeI GCATGC 2 cut(s) 1359, 2357
PaeR7I CTCGAG 1 cut(s) 1621
PagI TCATGA 1 cut(s) 391
PceI AGGCCT 1 cut(s) 325
PciI ACATGT 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PctI GAATGC 3 cut(s) 993, 1243, 1630
PflMI CCANNNNNTGG 4 cut(s) 135, 638, 1422, 2408
PleI GAGTC 2 cut(s) 563, 2667
PpsI GAGTC 2 cut(s) 563, 2667
PscI ACATGT 1 cut(s) 21
PsiI TTATAA 2 cut(s) 957, 2469
PspEI GGTNACC 1 cut(s) 530
PspN4I GGNNCC 6 cut(s) 644, 1666, 1667, 2179, 2576, 2832
PspOMI GGGCCC 1 cut(s) 1665
PspPI GGNCC 8 cut(s) 314, 465, 1466, 1665, 1666, 2582, 2831, 3004
PstI CTGCAG 5 cut(s) 354, 595, 829, 1236, 2925
PstNI CAGNNNCTG 2 cut(s) 1112, 2384
PsuI RGATCY 2 cut(s) 124, 1412
PvuII CAGCTG 3 cut(s) 1376, 2682, 2920
RsaI GTAC 6 cut(s) 907, 943, 1009, 1215, 2247, 2416
RsaNI GTAC 6 cut(s) 906, 942, 1008, 1214, 2246, 2415
RseI CAYNNNNRTG 2 cut(s) 264, 2094
Rsr2I CGGWCCG 1 cut(s) 465
RsrII CGGWCCG 1 cut(s) 465
SalI GTCGAC 1 cut(s) 2562
Sau3AI GATC 7 cut(s) 124, 178, 328, 408, 1300, 1412, 2038
Sau96I GGNCC 8 cut(s) 314, 465, 1466, 1665, 1666, 2582, 2831, 3004
ScaI AGTACT 2 cut(s) 907, 2416
SchI GAGTC 2 cut(s) 563, 2668
SduI GDGCHC 5 cut(s) 52, 259, 1669, 1868, 2730
SexAI ACCWGGT 1 cut(s) 1324
SfcI CTRYAG 8 cut(s) 282, 350, 591, 825, 1232, 2373, 2535, 2921
SfiI GGCCNNNNNGGCC 1 cut(s) 322
Sfr274I CTCGAG 1 cut(s) 1621
SfuI TTCGAA 1 cut(s) 1748
SinI GGWCC 3 cut(s) 465, 2582, 2831
SlaI CTCGAG 1 cut(s) 1621
SmiMI CAYNNNNRTG 2 cut(s) 264, 2094
SmlI CTYRAG 7 cut(s) 74, 173, 598, 1621, 1782, 2791, 2897
SmoI CTYRAG 7 cut(s) 74, 173, 598, 1621, 1782, 2791, 2897
SpeI ACTAGT 1 cut(s) 2417
SphI GCATGC 2 cut(s) 1359, 2357
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 4 cut(s) 725, 1986, 2070, 3007
SspI AATATT 1 cut(s) 1611
SspMI CTAG 4 cut(s) 1011, 2009, 2418, 2663
StuI AGGCCT 1 cut(s) 325
StyI CCWWGG 2 cut(s) 1908, 2578
TaiI ACGT 1 cut(s) 1222
TaqI TCGA 7 cut(s) 94, 207, 706, 1475, 1622, 1748, 2563
TatI WGTACW 3 cut(s) 905, 1213, 2414
TauI GCSGC 2 cut(s) 2072, 3009
TscAI CASTG 5 cut(s) 271, 643, 1057, 2409, 2808
TseFI GTSAC 2 cut(s) 530, 2169
Tsp45I GTSAC 2 cut(s) 530, 2169
TspGWI ACGGA 2 cut(s) 962, 971
TspRI CASTG 5 cut(s) 271, 643, 1057, 2409, 2808
Van91I CCANNNNNTGG 4 cut(s) 135, 638, 1422, 2408
Vha464I CTTAAG 1 cut(s) 2791
VpaK11BI GGWCC 3 cut(s) 465, 2582, 2831
XagI CCTNNNNNAGG 1 cut(s) 923
XapI RAATTY 6 cut(s) 114, 1096, 1418, 1760, 2254, 2275
XceI RCATGY 3 cut(s) 25, 1359, 2357
XcmI CCANNNNNNNNNTGG 1 cut(s) 2188
XhoI CTCGAG 1 cut(s) 1621
XmiI GTMKAC 3 cut(s) 1460, 2143, 2563
XspI CTAG 4 cut(s) 1011, 2009, 2418, 2663
ZrmI AGTACT 2 cut(s) 907, 2416
Zsp2I ATGCAT 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.