RLG00000034706

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008095
Physical Location & Seq
Forward (+)
52381949 .. 52387654
5706 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034706

Sequence Viewer

Length: 2643 bp
ATGTCTGCTCCTCCATCATGGGAGCGTGCCCAACGTTTGTTTGACAAGATTACTGAGTTGGAGAGTAGGCGTCGGAGGTCTGCCCAAGCACGAGTTCCCTCAGATCCTAATGCCTGGCAACAGATACGTGAGAATTATGAAGCTATAATTCTTGAGGACCATGCATTTTCAGAGCAGCACAATGTTGAGTATGCTTTGTGGCAGTTACATTACAAGCGGATTGACGAATTGCGCACGCACTTCAGTGCTGCTATGGCTTCTACAGGTTCAAACCCATCCCAGGGTTTGAAGGGACCCACTCGGCCTGATCGTATTACAAAAATAAGATTGCAGTTTAAAACATTCCTTTCAGAAGCAACTGGATTTTATCATGATTTAATAGTGAAGATCAGAGCAAAGTATGGGCTTCCTCTTGGCTATTTCTCTGAGGATTCTGAAAATCGAATTGTTATGGATAAGGATGGAAAGAAATCTGCTGATATGAAGAAAGGTTTGATATCATGCCACCGCTGTTTGATTTACTTGGGTGACCTTGCACGCTACAAAGGACTGTATGGGGAGGGGGACTCCAAAACTCGTGAGTATGCTGCTGCCTCAAGTTACTACTTGCAAGCTGCATCTTGTTTGCCGTCAAGTGGGAACCCTCATCATCAGCTTGCTATATTGGCTTCTTATTCGGGGGATGAGTTGGTGACTGTTTATCGATATTTTCGTAGCCTGGCCGTGGATAATCCCTTTTCAACTGCAAGGGATAACTTGATTGTGGCATTTGAGAAGAATCGTCAGAGTTACTCGCAACTGTCTGGCAATGCTAATGCTTCTGCACTCAAAGAGTTGCCTGCACGATTGACTGGCAAAGGTAGAGGGAAAGGGGAAGGAATACCTGCTAAAGACAACAACACAGTAGCAGGCCTTGTTAAGGAAAGATCATCCAGTAATCAGGAAAAATACAAAGCTTTTTCAATTCGTTTTGTCCGTCTAAATGGCATTCTTTTTACACGTACTAGCCTGGAGACATTTGCAGAAGTTCTCTCTGTTGTAAGCAGTGGTTTATATGAGCTTCTATCTTCTGGAGCAGAAGAGGAGCTGAGTCTTGGTGCTGATGCTGTTGAGAATGGACTGGTCATTGTTCGACTGGTCTCTATACTGATATTCACAGTTCATAATGTGAAAAAGGAAAGTGAAGGTCAGTCATATGCAGAAATTGTACAGCGTGCTGTTCTGCTGCAAAATGCAATTACTGCTGTCTTCGAGTTGATGGGGCATGTCTTAGAGAGATGCATGAAGCTGGGGGATCCAACTTCAAGTCACCTGTTACCAGGCATTCTGGTTTTTGTAGAATGGTTGGCATGTTGTCCTGATGTAGCAGCTGGCAGTGATGCAGATGAAAAACAGTCTTCTGTTAGAGCAAAGTTCTGGAATGCTTGCATACACTTGTTGAATAATCTCTCATCATCTGTTCCGATGTCCATAGATGATGATGGAGATGAGACTTGCTTTAATAATATGAGCAGATATGAAGAAGGGGAAACTGAGAATCGCCTTGCTTTGTGGGAGGATTTTGAGTTGAGAGGATTCATGCCACTTCTTCCAGCACAGACAATATTGGATTTCTCGAGGAAGCATTCCTTTGGAAGTGATGGCCAGAAGGAAAAAGGTGCGCGTGTTAAAAGGATCCTGGCGGCAGGCAAGGCTTTAGCAAATGTGGTCAAGGTTGATCAAAAAGCCATTTATTTTGATTCCCAGACAAAGAAATTTGTCATTGGTGTTGAGCCTTCAATGAATGGCGATAATATGCCTACCTCCTTTTTAGGCATGGCTAATTCCAATGATAATCTGGAAGAAAATCAAGTGGAGGACACCAAAAATCTGGGAGGTCCATTTCAAAAGCCAGAGTTGACCATGGATGGGGATGAGGAAGATGAAGTAATAGTTTTTAAACCAATAGTGGCCGAGAAGAGGCCTGATGTGGTAGGCACTACATGGGCTATCCCCCAGCATTTGGAACCATTTAAATCAGAAGAAATATCTCTTGCTAACAACCTAAAGAGTTTGGGATTTATGGGCAATGGGCAAGTACTGAAATCTGAACATGTATCAAGTTCGGTTCCATTCCAACAGCCTGTTAATGGTAGTACGAGTAGTATGTTTTACAGTCATGCAAAGGCTCCAGAAGCTGTGTTGCCGTTTAAGGTTGATACTATTGCATCTTCTGGACCATTTGCGGATAGTTTGACATTGAAGACGTCATCAGCTTTGCCCGCTGCTATCCGAAAAAATCCAGTTAGTCGACCTGTTAGACACCTTGCTGGTTGGATGGGTATCAGGGCCCATCTATGCCATTCCAAGCAGAAAACCAGAAAAGCCGGCAAGAATTCCATATGCTCGATGATCTTAAACTTCACCATGAAATGCAGCTGCAGCAGCAGCAGCAACAACCTGAGCAATATCAAGGACAGTCTGTCTGGACAGGCCGCTATTTTGTGTGATGCCAGTATCAATGTGATGGTTCTTGAGGATGATTTGGTGAATCATTGGTGGTTTGGCTTCTTGCTGGCGCTTACAAATACCCAGGTTTTGCCAAGCTCTCCAGCCCAGGGGATGACAAATATGGATATATGTTTCATGGTGCGAAATTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

881

Amino Acids

97.25

Weight (kDa)

6.72

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 62 - 185 2.7e-17 Telomerase activating protein Est1
EST1_DNA_bind PF10373 198 - 531 2.7e-63 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2249
Acc16I TGCGCA 1 cut(s) 233
Acc36I ACCTGC 1 cut(s) 892
AccB7I CCANNNNNTGG 1 cut(s) 2002
AccI GTMKAC 1 cut(s) 2290
AccII CGCG 1 cut(s) 1663
AciI CCGC 6 cut(s) 217, 508, 1682, 2225, 2262, 2475
AclI AACGTT 1 cut(s) 34
AclWI GGATC 5 cut(s) 98, 1289, 1302, 1669, 1682
AcoI YGGCCR 3 cut(s) 720, 1642, 1950
AcsI RAATTY 3 cut(s) 1754, 2374, 2635
AcuI CTGAAG 1 cut(s) 226
AcyI GRCGYC 2 cut(s) 70, 2246
AfaI GTAC 4 cut(s) 1003, 1209, 2079, 2137
AflIII ACRYGT 2 cut(s) 998, 2092
AgsI TTSAA 9 cut(s) 270, 289, 741, 963, 1305, 1441, 1779, 1886, 2242
AhdI GACNNNNNGTC 1 cut(s) 2461
AjnI CCWGG 8 cut(s) 113, 279, 717, 1008, 1318, 1677, 2571, 2595
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AleI CACNNNNGTG 1 cut(s) 243
Alw26I GTCTC 3 cut(s) 1007, 1144, 1484
AlwI GGATC 5 cut(s) 98, 1289, 1302, 1669, 1682
AlwNI CAGNNNCTG 1 cut(s) 2177
Ama87I CYCGRG 1 cut(s) 1615
AoxI GGCC 8 cut(s) 302, 720, 910, 1642, 1950, 1961, 2328, 2472
ApaI GGGCCC 1 cut(s) 2332
ApoI RAATTY 3 cut(s) 1754, 2374, 2635
AspLEI GCGC 3 cut(s) 234, 1663, 2560
AspS9I GGNCC 6 cut(s) 157, 293, 1877, 2216, 2328, 2329
AsuHPI GGTGA 5 cut(s) 539, 703, 1301, 2395, 2539
AvaI CYCGRG 1 cut(s) 1615
AvaII GGWCC 4 cut(s) 157, 293, 1877, 2216
BaeGI GKGCMC 2 cut(s) 31, 2332
BalI TGGCCA 1 cut(s) 1644
BamHI GGATCC 2 cut(s) 1294, 1674
BanII GRGCYC 1 cut(s) 2332
BauI CACGAG 2 cut(s) 90, 576
BbsI GAAGAC 3 cut(s) 1240, 1389, 2249
BceAI ACGGC 3 cut(s) 613, 707, 2170
BciT130I CCWGG 8 cut(s) 115, 281, 719, 1010, 1320, 1679, 2573, 2597
BclI TGATCA 1 cut(s) 1717
BcoDI GTCTC 3 cut(s) 1007, 1144, 1484
BfaI CTAG 1 cut(s) 1005
BfmI CTRYAG 2 cut(s) 261, 2419
BfoI RGCGCY 1 cut(s) 2561
BfuAI ACCTGC 1 cut(s) 892
BmcAI AGTACT 1 cut(s) 2079
Bme1390I CCNGG 8 cut(s) 115, 281, 719, 1010, 1320, 1679, 2573, 2597
Bme18I GGWCC 4 cut(s) 157, 293, 1877, 2216
BmeRI GACNNNNNGTC 1 cut(s) 2461
BmeT110I CYCGRG 1 cut(s) 1615
BmgT120I GGNCC 6 cut(s) 157, 293, 1877, 2216, 2328, 2329
BmiI GGNNCC 9 cut(s) 294, 295, 641, 1296, 1676, 2007, 2109, 2169, 2330
BmrFI CCNGG 8 cut(s) 115, 281, 719, 1010, 1320, 1679, 2573, 2597
BmsI GCATC 6 cut(s) 626, 1093, 1268, 1369, 2216, 2479
BpiI GAAGAC 3 cut(s) 1240, 1389, 2249
BplI GAGNNNNNCTC 2 cut(s) 551, 583
BpmI CTGGAG 4 cut(s) 1031, 1092, 2154, 2574
Bpu10I CCTNAGC 1 cut(s) 2441
BpuEI CTTGAG 3 cut(s) 173, 580, 2534
Bsa29I ATCGAT 1 cut(s) 703
BsaAI YACGTR 2 cut(s) 128, 1001
BsaBI GATNNNNATC 1 cut(s) 2321
BsaHI GRCGYC 2 cut(s) 70, 2246
BsaI GGTCTC 1 cut(s) 1144
BsaJI CCNNGG 7 cut(s) 279, 280, 723, 1902, 2571, 2595, 2596
Bse118I RCCGGY 1 cut(s) 2366
Bse1I ACTGG 7 cut(s) 364, 856, 933, 1125, 1140, 2282, 2493
Bse3DI GCAATG 2 cut(s) 814, 2074
Bse8I GATNNNNATC 1 cut(s) 2321
BseBI CCWGG 8 cut(s) 115, 281, 719, 1010, 1320, 1679, 2573, 2597
BseCI ATCGAT 1 cut(s) 703
BseDI CCNNGG 7 cut(s) 279, 280, 723, 1902, 2571, 2595, 2596
BseGI GGATG 9 cut(s) 275, 466, 688, 929, 1912, 1918, 2322, 2524, 2607
BseJI GATNNNNATC 1 cut(s) 2321
BseMI GCAATG 2 cut(s) 814, 2074
BseMII CTCAG 6 cut(s) 45, 114, 417, 1079, 1524, 2432
BseNI ACTGG 7 cut(s) 364, 856, 933, 1125, 1140, 2282, 2493
BseRI GAGGAG 1 cut(s) 1097
BseSI GKGCMC 2 cut(s) 31, 2332
BseYI CCCAGC 2 cut(s) 1288, 1995
BsgI GTGCAG 2 cut(s) 807, 825
Bsh1236I CGCG 1 cut(s) 1663
BshFI GGCC 8 cut(s) 304, 722, 912, 1644, 1952, 1963, 2330, 2474
BshVI ATCGAT 1 cut(s) 703
BsiHKCI CYCGRG 1 cut(s) 1615
BsiSI CCGG 1 cut(s) 2367
BslFI GGGAC 2 cut(s) 306, 578
BsmAI GTCTC 3 cut(s) 1007, 1144, 1484
BsmFI GGGAC 2 cut(s) 306, 578
BsmI GAATGC 4 cut(s) 987, 1323, 1426, 1624
BsnI GGCC 8 cut(s) 304, 722, 912, 1644, 1952, 1963, 2330, 2474
Bso31I GGTCTC 1 cut(s) 1144
BsoBI CYCGRG 1 cut(s) 1615
Bsp120I GGGCCC 1 cut(s) 2328
Bsp1286I GDGCHC 2 cut(s) 31, 2332
Bsp1407I TGTACA 1 cut(s) 1207
Bsp143I GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2391
Bsp19I CCATGG 1 cut(s) 1902
BspACI CCGC 6 cut(s) 217, 508, 1682, 2225, 2262, 2475
BspANI GGCC 8 cut(s) 304, 722, 912, 1644, 1952, 1963, 2330, 2474
BspCNI CTCAG 6 cut(s) 46, 113, 418, 1080, 1525, 2433
BspDI ATCGAT 1 cut(s) 703
BspFNI CGCG 1 cut(s) 1663
BspHI TCATGA 1 cut(s) 370
BspLI GGNNCC 9 cut(s) 294, 295, 641, 1296, 1676, 2007, 2109, 2169, 2330
BspMAI CTGCAG 1 cut(s) 2423
BspMI ACCTGC 1 cut(s) 892
BspPI GGATC 5 cut(s) 98, 1289, 1302, 1669, 1682
BspTNI GGTCTC 1 cut(s) 1144
BsrDI GCAATG 2 cut(s) 814, 2074
BsrFI RCCGGY 1 cut(s) 2366
BsrGI TGTACA 1 cut(s) 1207
BsrI ACTGG 7 cut(s) 364, 856, 933, 1125, 1140, 2282, 2493
BssAI RCCGGY 1 cut(s) 2366
BssECI CCNNGG 7 cut(s) 279, 280, 723, 1902, 2571, 2595, 2596
BssMI GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2391
BssNI GRCGYC 2 cut(s) 70, 2246
BssSI CACGAG 2 cut(s) 90, 576
BssT1I CCWWGG 1 cut(s) 1902
Bst2BI CACGAG 2 cut(s) 90, 576
Bst2UI CCWGG 8 cut(s) 115, 281, 719, 1010, 1320, 1679, 2573, 2597
Bst4CI ACNGT 8 cut(s) 552, 697, 801, 904, 1159, 1395, 2156, 2459
Bst6I CTCTTC 2 cut(s) 1074, 1952
BstACI GRCGYC 2 cut(s) 70, 2246
BstAPI GCANNNNNTGC 1 cut(s) 1241
BstAUI TGTACA 1 cut(s) 1207
BstBAI YACGTR 2 cut(s) 128, 1001
BstDEI CTNAG 7 cut(s) 54, 100, 426, 1088, 1270, 1533, 2441
BstDSI CCRYGG 2 cut(s) 723, 1902
BstEII GGTNACC 1 cut(s) 527
BstENI CCTNNNNNAGG 1 cut(s) 917
BstF5I GGATG 9 cut(s) 275, 466, 688, 929, 1912, 1918, 2322, 2524, 2607
BstFNI CGCG 1 cut(s) 1663
BstH2I RGCGCY 1 cut(s) 2561
BstHHI GCGC 3 cut(s) 234, 1663, 2560
BstKTI GATC 8 cut(s) 106, 310, 390, 929, 1297, 1677, 1720, 2394
BstMAI GTCTC 3 cut(s) 1007, 1144, 1484
BstMBI GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2391
BstNI CCWGG 8 cut(s) 115, 281, 719, 1010, 1320, 1679, 2573, 2597
BstNSI RCATGY 3 cut(s) 1268, 1353, 2096
BstPI GGTNACC 1 cut(s) 527
BstSCI CCNGG 8 cut(s) 113, 279, 717, 1008, 1318, 1677, 2571, 2595
BstSFI CTRYAG 2 cut(s) 261, 2419
BstSLI GKGCMC 2 cut(s) 31, 2332
BstUI CGCG 1 cut(s) 1663
BstV2I GAAGAC 3 cut(s) 1240, 1389, 2249
BstX2I RGATCY 3 cut(s) 103, 1294, 1674
BstXI CCANNNNNNTGG 1 cut(s) 1870
BstYI RGATCY 3 cut(s) 103, 1294, 1674
Bsu15I ATCGAT 1 cut(s) 703
BsuRI GGCC 8 cut(s) 304, 722, 912, 1644, 1952, 1963, 2330, 2474
BsuTUI ATCGAT 1 cut(s) 703
BtgI CCRYGG 2 cut(s) 723, 1902
BtsCI GGATG 9 cut(s) 275, 466, 688, 929, 1912, 1918, 2322, 2524, 2607
BtsI GCAGTG 2 cut(s) 1051, 1381
BtsIMutI CAGTG 3 cut(s) 250, 1051, 1381
BveI ACCTGC 1 cut(s) 892
CaiI CAGNNNCTG 1 cut(s) 2177
CciI TCATGA 1 cut(s) 370
CfoI GCGC 3 cut(s) 234, 1663, 2560
Cfr10I RCCGGY 1 cut(s) 2366
Cfr13I GGNCC 6 cut(s) 157, 293, 1877, 2216, 2328, 2329
ClaI ATCGAT 1 cut(s) 703
CseI GACGC 1 cut(s) 59
Csp6I GTAC 4 cut(s) 1002, 1208, 2078, 2136
CviQI GTAC 4 cut(s) 1002, 1208, 2078, 2136
DdeI CTNAG 7 cut(s) 54, 100, 426, 1088, 1270, 1533, 2441
DpnI GATC 8 cut(s) 105, 309, 389, 928, 1296, 1676, 1719, 2393
DpnII GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2391
DraI TTTAAA 3 cut(s) 337, 1939, 2014
DriI GACNNNNNGTC 1 cut(s) 2461
EaeI YGGCCR 3 cut(s) 720, 1642, 1950
Eam1104I CTCTTC 2 cut(s) 1074, 1952
Eam1105I GACNNNNNGTC 1 cut(s) 2461
EarI CTCTTC 2 cut(s) 1074, 1952
Eco130I CCWWGG 1 cut(s) 1902
Eco147I AGGCCT 2 cut(s) 912, 1963
Eco24I GRGCYC 1 cut(s) 2332
Eco31I GGTCTC 1 cut(s) 1144
Eco32I GATATC 1 cut(s) 498
Eco47I GGWCC 4 cut(s) 157, 293, 1877, 2216
Eco57I CTGAAG 1 cut(s) 226
Eco88I CYCGRG 1 cut(s) 1615
Eco91I GGTNACC 1 cut(s) 527
EcoNI CCTNNNNNAGG 1 cut(s) 917
EcoO109I RGGNCCY 2 cut(s) 293, 2328
EcoO65I GGTNACC 1 cut(s) 527
EcoRI GAATTC 1 cut(s) 2374
EcoRII CCWGG 8 cut(s) 113, 279, 717, 1008, 1318, 1677, 2571, 2595
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 1902
EcoT22I ATGCAT 2 cut(s) 166, 1283
EcoT38I GRGCYC 1 cut(s) 2332
ErhI CCWWGG 1 cut(s) 1902
FalI AAGNNNNNCTT 2 cut(s) 1613, 1645
FaqI GGGAC 2 cut(s) 306, 578
FauI CCCGC 1 cut(s) 2269
FauNDI CATATG 2 cut(s) 1195, 2381
FbaI TGATCA 1 cut(s) 1717
FblI GTMKAC 1 cut(s) 2290
FokI GGATG 9 cut(s) 262, 473, 695, 916, 1919, 1925, 2329, 2531, 2614
FriOI GRGCYC 1 cut(s) 2332
FspBI CTAG 1 cut(s) 1005
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 3 cut(s) 233, 1662, 2559
GsaI CCCAGC 2 cut(s) 1292, 1999
GsuI CTGGAG 4 cut(s) 1031, 1092, 2154, 2574
HaeII RGCGCY 1 cut(s) 2561
HaeIII GGCC 8 cut(s) 304, 722, 912, 1644, 1952, 1963, 2330, 2474
HapII CCGG 1 cut(s) 2367
HgaI GACGC 1 cut(s) 59
HhaI GCGC 3 cut(s) 234, 1663, 2560
Hin1I GRCGYC 2 cut(s) 70, 2246
Hin6I GCGC 3 cut(s) 232, 1661, 2558
HinP1I GCGC 3 cut(s) 232, 1661, 2558
HincII GTYRAC 2 cut(s) 1899, 2291
HindII GTYRAC 2 cut(s) 1899, 2291
HindIII AAGCTT 1 cut(s) 954
HinfI GANTC 8 cut(s) 431, 566, 778, 1090, 1537, 1575, 1739, 2530
HpaII CCGG 1 cut(s) 2367
HphI GGTGA 5 cut(s) 539, 703, 1301, 2395, 2539
Hpy166II GTNNAC 2 cut(s) 1899, 2291
Hpy8I GTNNAC 2 cut(s) 1899, 2291
Hpy99I CGWCG 1 cut(s) 75
HpyAV CCTTC 6 cut(s) 283, 869, 1178, 1517, 1642, 1785
HpyCH4III ACNGT 8 cut(s) 552, 697, 801, 904, 1159, 1395, 2156, 2459
HpyCH4IV ACGT 4 cut(s) 34, 127, 1000, 2246
HpyF3I CTNAG 7 cut(s) 54, 100, 426, 1088, 1270, 1533, 2441
HpySE526I ACGT 4 cut(s) 34, 127, 1000, 2246
Hsp92I GRCGYC 2 cut(s) 70, 2246
HspAI GCGC 3 cut(s) 232, 1661, 2558
KflI GGGWCCC 1 cut(s) 293
KroI GCCGGC 1 cut(s) 2366
KroNI GCCGGC 1 cut(s) 2368
Ksp22I TGATCA 1 cut(s) 1717
Kzo9I GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2391
LmnI GCTCC 5 cut(s) 13, 22, 1073, 1084, 2173
LweI GCATC 6 cut(s) 626, 1093, 1268, 1369, 2216, 2479
MaeI CTAG 1 cut(s) 1005
MaeII ACGT 4 cut(s) 34, 127, 1000, 2246
MaeIII GTNAC 7 cut(s) 204, 527, 599, 691, 788, 1307, 1314
MalI GATC 8 cut(s) 105, 309, 389, 928, 1296, 1676, 1719, 2393
MboI GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2391
MflI RGATCY 3 cut(s) 103, 1294, 1674
MhlI GDGCHC 2 cut(s) 31, 2332
MlsI TGGCCA 1 cut(s) 1644
MluNI TGGCCA 1 cut(s) 1644
MlyI GAGTC 2 cut(s) 560, 1099
MmeI TCCRAC 5 cut(s) 39, 53, 1322, 2140, 2294
Mox20I TGGCCA 1 cut(s) 1644
Mph1103I ATGCAT 2 cut(s) 166, 1283
MroNI GCCGGC 1 cut(s) 2366
MscI TGGCCA 1 cut(s) 1644
MslI CAYNNNNRTG 1 cut(s) 243
Msp20I TGGCCA 1 cut(s) 1644
MspA1I CMGCKG 4 cut(s) 510, 1370, 2264, 2418
MspI CCGG 1 cut(s) 2367
MspR9I CCNGG 8 cut(s) 115, 281, 719, 1010, 1320, 1679, 2573, 2597
Mva1269I GAATGC 4 cut(s) 987, 1323, 1426, 1624
MvaI CCWGG 8 cut(s) 115, 281, 719, 1010, 1320, 1679, 2573, 2597
MvnI CGCG 1 cut(s) 1663
NaeI GCCGGC 1 cut(s) 2368
NcoI CCATGG 1 cut(s) 1902
NdeI CATATG 2 cut(s) 1195, 2381
NdeII GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2391
NgoMIV GCCGGC 1 cut(s) 2366
NlaIV GGNNCC 9 cut(s) 294, 295, 641, 1296, 1676, 2007, 2109, 2169, 2330
NmeAIII GCCGAG 2 cut(s) 280, 1978
NmuCI GTSAC 3 cut(s) 527, 691, 1307
NsbI TGCGCA 1 cut(s) 233
NsiI ATGCAT 2 cut(s) 166, 1283
NspI RCATGY 3 cut(s) 1268, 1353, 2096
OliI CACNNNNGTG 1 cut(s) 243
PaeR7I CTCGAG 1 cut(s) 1615
PagI TCATGA 1 cut(s) 370
PasI CCCWGGG 2 cut(s) 280, 2596
PceI AGGCCT 2 cut(s) 912, 1963
PciI ACATGT 1 cut(s) 2092
PcsI WCGNNNNNNNCGW 3 cut(s) 307, 709, 973
PctI GAATGC 4 cut(s) 987, 1323, 1426, 1624
PdiI GCCGGC 1 cut(s) 2368
PfeI GAWTC 6 cut(s) 431, 778, 1537, 1575, 1739, 2530
PflMI CCANNNNNTGG 1 cut(s) 2002
PleI GAGTC 2 cut(s) 560, 1098
PpsI GAGTC 2 cut(s) 560, 1098
Ppu21I YACGTR 2 cut(s) 128, 1001
PpuMI RGGWCCY 1 cut(s) 293
PscI ACATGT 1 cut(s) 2092
Psp1406I AACGTT 1 cut(s) 34
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 8 cut(s) 113, 279, 717, 1008, 1318, 1677, 2571, 2595
PspEI GGTNACC 1 cut(s) 527
PspFI CCCAGC 2 cut(s) 1288, 1995
PspGI CCWGG 8 cut(s) 113, 279, 717, 1008, 1318, 1677, 2571, 2595
PspN4I GGNNCC 9 cut(s) 294, 295, 641, 1296, 1676, 2007, 2109, 2169, 2330
PspOMI GGGCCC 1 cut(s) 2328
PspPI GGNCC 6 cut(s) 157, 293, 1877, 2216, 2328, 2329
PspPPI RGGWCCY 1 cut(s) 293
PstI CTGCAG 1 cut(s) 2423
PstNI CAGNNNCTG 1 cut(s) 2177
PsuI RGATCY 3 cut(s) 103, 1294, 1674
PvuII CAGCTG 2 cut(s) 1370, 2418
RsaI GTAC 4 cut(s) 1003, 1209, 2079, 2137
RsaNI GTAC 4 cut(s) 1002, 1208, 2078, 2136
RseI CAYNNNNRTG 1 cut(s) 243
SalI GTCGAC 1 cut(s) 2289
Sau3AI GATC 8 cut(s) 103, 307, 387, 926, 1294, 1674, 1717, 2391
Sau96I GGNCC 6 cut(s) 157, 293, 1877, 2216, 2328, 2329
ScaI AGTACT 1 cut(s) 2079
SchI GAGTC 2 cut(s) 560, 1099
ScrFI CCNGG 8 cut(s) 115, 281, 719, 1010, 1320, 1679, 2573, 2597
SduI GDGCHC 2 cut(s) 31, 2332
SfaNI GCATC 6 cut(s) 626, 1093, 1268, 1369, 2216, 2479
SfcI CTRYAG 2 cut(s) 261, 2419
Sfr274I CTCGAG 1 cut(s) 1615
SinI GGWCC 4 cut(s) 157, 293, 1877, 2216
SlaI CTCGAG 1 cut(s) 1615
SmiI ATTTAAAT 1 cut(s) 2014
SmiMI CAYNNNNRTG 1 cut(s) 243
SmlI CTYRAG 4 cut(s) 152, 595, 1615, 2513
SmoI CTYRAG 4 cut(s) 152, 595, 1615, 2513
SseBI AGGCCT 2 cut(s) 912, 1963
SsiI CCGC 6 cut(s) 217, 508, 1682, 2225, 2262, 2475
SspI AATATT 1 cut(s) 1605
SspMI CTAG 1 cut(s) 1005
StuI AGGCCT 2 cut(s) 912, 1963
StyD4I CCNGG 8 cut(s) 113, 279, 717, 1008, 1318, 1677, 2571, 2595
StyI CCWWGG 1 cut(s) 1902
SwaI ATTTAAAT 1 cut(s) 2014
TaaI ACNGT 8 cut(s) 552, 697, 801, 904, 1159, 1395, 2156, 2459
TaiI ACGT 4 cut(s) 37, 130, 1003, 2249
TaqI TCGA 7 cut(s) 442, 703, 1132, 1251, 1616, 2290, 2387
TatI WGTACW 2 cut(s) 1207, 2077
TauI GCSGC 2 cut(s) 1685, 2477
TfiI GAWTC 6 cut(s) 431, 778, 1537, 1575, 1739, 2530
TscAI CASTG 3 cut(s) 250, 1051, 1381
TseFI GTSAC 3 cut(s) 527, 691, 1307
Tsp45I GTSAC 3 cut(s) 527, 691, 1307
TspGWI ACGGA 1 cut(s) 965
TspRI CASTG 3 cut(s) 250, 1051, 1381
Van91I CCANNNNNTGG 1 cut(s) 2002
VpaK11BI GGWCC 4 cut(s) 157, 293, 1877, 2216
XagI CCTNNNNNAGG 1 cut(s) 917
XapI RAATTY 3 cut(s) 1754, 2374, 2635
XceI RCATGY 3 cut(s) 1268, 1353, 2096
XcmI CCANNNNNNNNNTGG 1 cut(s) 1834
XhoI CTCGAG 1 cut(s) 1615
XmiI GTMKAC 1 cut(s) 2290
XspI CTAG 1 cut(s) 1005
ZraI GACGTC 1 cut(s) 2247
ZrmI AGTACT 1 cut(s) 2079
Zsp2I ATGCAT 2 cut(s) 166, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.