Rh5CG371000

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008095
Physical Location & Seq
Forward (+)
47478148 .. 47482530
4383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG371000.1

Sequence Viewer

Length: 2742 bp
ATGATGATCACAAAATTGGATAAAATGTCTGCTTCTTCTCCATCATGGGAGCGTGCCCAACGTTTGTTCGACAAGATTACTGAGTTGGAGAGTAGGCGTCGGAGGTCTGCCCAAGCACGAGTTCCCTCAGATCCTAATGCCTGGCAACAGATACGTGAGAATTATGAAGCTATAATTCTTGAGGACCATGCATTTTCAGAGCAGCACAATGTTGAGTATGCTTTGTGGCAGTTACATTACAAGCGGATTGACGAATTGCGCACGCACTTCAGTGCTGCTATGGCTTCTACAGGTTCAAACTCATCCCAAGGTTTGAAGGGACCTACTCGGCCTGATCGTATTACAAAAATAAGATTGCAGTTTAAAACATTCCTTTCAGAAGCGACTGGATTTTATCATGATTTAATAGTGAAGATCAGAGCAAAGTATGGGCTTCCTCTTGGCTATTTCTCTGAGGATTCTGAAAATCGAATTGTTATGGATAAGGATGGAAAGAAATCTGCTGATATGAAGAAAGGTTTGATATCATGCCACCGCTGTTTGATTTACTTGGGTGACCTTGCACGCTACAAAGGACTGTATGGGGAGGGGGACTCCAAAACTCGTGAGTATGCTGCTGCCTCAAGTTACTACTTGCAAGCTGCATCTTGTTTGCCATCAAGTGGGAACCCTCATCATCAGCTTGCTATATTGGCTTCTTATTCGGGGGATGAGTTGGTGACTGTTTATCGATATTTTCGTAGCCTGGCCGTGGATAATCCCTTTTCAACTGCAAGGGATAACTTGATTGTGGCATTTGAGAAGAATCGTCAGAGTTACTCGCAACTGTCTGGCAATGCTAATGCTTCTGCACTCAAAGAGTTGCCTGCACGATTGACTGGCAAAGGTAGAGGGAAAGGGGAAGCAATACCTGCTAAAGTTAATAACACAGAAGCAGGCCTTGTTAAGGAAAGATCATCCAGTAATCAGGAAAAATACAAAGCTTTTTCAATTCGTTTTGTCCGTCTAAATGGCATTCTTTTTACACGTACTAGCCTGGAGACATTTGCAGAAGTTCTCTCTGTTGTAAGCAGTGGTTTATATGAGCTTCTATCTTCTGGAGCAGAAGAGGAGCTGAGTCTTGGTGCTGATGCTGTTGAGAATGGACTGGTCATTGTTCGACTGGTCTCTATACTGATATTCACAGTTCATAATGTGAAAAAGGAAAGTGAAGGTCAGTCATATGCAGAAATTGTACAGCGTGCTGTTCTGCTGCAAAATGCATTTACTGCTGTCTTCGAGTTGATGGGGCATGTCTTAGAGAGATGCGTGAAGCTGGGGGATCCAACTTCAAGTCACCTGTTACCAGGCATTCTGGTTTTTGTAGAATGGTTGGCATGTTGTCCTGATGTAGCAGCTGGCAGTGATGCAGATGAAAAACAGTCATCTGTTAGAGCAAAGTTCTGGAATGCTTGCATACACTTGTTGAATAATCTCTTATCATCTGTTCCGATGTCCATAGATGATGATGGAGATGAGACTTGCTTTAATAATATGAGCAGATATGAAGAAGGGGAAACTGAGAATCGCCTTGCTTTGTGGGAGGATTTTGAGTTGAGAGGATTCATGCCACTTCTTCCAGCACAGACAATATTGGATTTCTCGAGGAAGCATTCTTTTGGAAGTGATGGCCAGAAGGAAAAAGGTGCGCGTCTTAAAAGGATCCTGGCGGCAGGCAAGGCTTTAGCAAATGTGGTCAAGGTTGATCAAAAAGCCATTTATTTTGATTCCCAGACAAAGAAATTTGTCATCGGTGTTGAGCCTTCAATGAATGGTGATTATATGCCTACCTCCTTTTTAGGCATGGCTAATTCCAATGATAATCTGGAAGAAAATCAAGTGGAGGACACCAAAAATCTGGGAGGTCCATTTCAAAAGCCAGAGTTGACCATGGATGGGGATGAGGAAGATGAAGTAATAGTTTTTAAACCAATAGTGGCTGAGAAGAGGCCTGATGTGGTAGGCACTACATGGGCTATCCCCCAGCATTTGGAACCATTTAAATCAGAAGAAATATCTCTTGCTAACAACCTAAAGAGTTTGGGATTTATGGGCAATGGGCAAGTACTGAAATCTGACCATGTATCAAGTTCGGTTCCATTCCAACAGCCTGTTAATGGTAGTACTAGTAGTATGTTTTACAGCAATGCAAAGGCTCCAGAAGCTGTGTTGCCGTTTAAGGTTGATACTATTGCATCTGCTGGACCATTTGCGGATGGTTTGACATTGAAGACGTCATCAGCTTTGCCCGCTGCTATCCGAAAAAATCCAGTTAGTCGACCTGTTAGACACCTTGGTCCGCCACCTGGCTTTAGCCATGTTCCCGCTAAACAAGTGAACGAGTCTATTTATAATTCAGAGTCAATGGGTGAGAATCCACTGATGGATGATTACAGCTGGTTGGATGGGTATCAGGTGCCATCTTCAACTAAAGGAAATGCATTCAGCAGTTCCATTAACTACTCATCTCATTCAAATCTCCATCGCGTTCCTAATGGGAATGGCTTAAGTGGAACAGTGAACTTTCCCTTTCCTGGTAAACAGGGCCCATCTATGCCATTCCAAGCAGAAAACCAGAAAAGCCGGCAAGAATTCCATATGCTCGATGATCTTAAACTTCACCATGAAATGCAGCTGCAGCAGCAGCAACAACCTGAGCAATATCAAGGACAGTCTGTCTGGACAGGCCGCTATTTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

913

Amino Acids

101.36

Weight (kDa)

6.23

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 71 - 194 2.5e-17 Telomerase activating protein Est1
EST1_DNA_bind PF10373 207 - 540 4.2e-64 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2391
AasI GACNNNNNNGTC 1 cut(s) 2334
AatII GACGTC 1 cut(s) 2276
Acc16I TGCGCA 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 919
AccB1I GGYRCC 1 cut(s) 2455
AccB7I CCANNNNNTGG 2 cut(s) 662, 2029
AccI GTMKAC 1 cut(s) 2317
AccII CGCG 2 cut(s) 1690, 2526
AciI CCGC 8 cut(s) 244, 535, 1709, 2252, 2289, 2339, 2364, 2728
AclI AACGTT 1 cut(s) 61
AclWI GGATC 5 cut(s) 125, 1316, 1329, 1696, 1709
AcoI YGGCCR 2 cut(s) 747, 1669
AcsI RAATTY 2 cut(s) 1781, 2630
AcuI CTGAAG 1 cut(s) 253
AcyI GRCGYC 2 cut(s) 97, 2273
AfaI GTAC 4 cut(s) 1030, 1236, 2106, 2164
AfiI CCNNNNNNNGG 8 cut(s) 662, 751, 946, 1935, 2029, 2156, 2345, 2573
AflII CTTAAG 1 cut(s) 2545
AflIII ACRYGT 1 cut(s) 1025
AhdI GACNNNNNGTC 1 cut(s) 2714
AhlI ACTAGT 1 cut(s) 2165
AjnI CCWGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
AjuI GAANNNNNNNTTGG 2 cut(s) 105, 137
AleI CACNNNNGTG 1 cut(s) 270
Alw26I GTCTC 3 cut(s) 1034, 1171, 1511
AlwI GGATC 5 cut(s) 125, 1316, 1329, 1696, 1709
AlwNI CAGNNNCTG 1 cut(s) 2204
Ama87I CYCGRG 1 cut(s) 1642
AoxI GGCC 7 cut(s) 329, 747, 937, 1669, 1988, 2584, 2725
ApaI GGGCCC 1 cut(s) 2588
ApoI RAATTY 2 cut(s) 1781, 2630
AspLEI GCGC 2 cut(s) 261, 1690
AspS9I GGNCC 7 cut(s) 184, 320, 1904, 2243, 2336, 2584, 2585
AsuHPI GGTGA 6 cut(s) 566, 730, 1328, 1826, 2420, 2651
AvaI CYCGRG 1 cut(s) 1642
AvaII GGWCC 5 cut(s) 184, 320, 1904, 2243, 2336
BaeGI GKGCMC 2 cut(s) 58, 2588
BalI TGGCCA 1 cut(s) 1671
BamHI GGATCC 2 cut(s) 1321, 1701
BanI GGYRCC 1 cut(s) 2455
BanII GRGCYC 1 cut(s) 2588
BauI CACGAG 2 cut(s) 117, 603
BbsI GAAGAC 2 cut(s) 1267, 2276
BceAI ACGGC 2 cut(s) 734, 2197
BciT130I CCWGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
BclI TGATCA 2 cut(s) 6, 1744
BcoDI GTCTC 3 cut(s) 1034, 1171, 1511
BcuI ACTAGT 1 cut(s) 2165
BfaI CTAG 2 cut(s) 1032, 2166
BfmI CTRYAG 2 cut(s) 288, 2675
BfrI CTTAAG 1 cut(s) 2545
BfuAI ACCTGC 1 cut(s) 919
BmcAI AGTACT 2 cut(s) 2106, 2164
Bme1390I CCNGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
Bme18I GGWCC 5 cut(s) 184, 320, 1904, 2243, 2336
BmeRI GACNNNNNGTC 1 cut(s) 2714
BmeT110I CYCGRG 1 cut(s) 1642
BmgT120I GGNCC 7 cut(s) 184, 320, 1904, 2243, 2336, 2584, 2585
BmiI GGNNCC 9 cut(s) 321, 668, 1323, 1703, 2034, 2136, 2196, 2457, 2586
BmrFI CCNGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
BmsI GCATC 5 cut(s) 653, 1120, 1295, 1396, 2243
BpiI GAAGAC 2 cut(s) 1267, 2276
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 3 cut(s) 1058, 1119, 2181
Bpu10I CCTNAGC 1 cut(s) 2694
BpuEI CTTGAG 2 cut(s) 200, 607
Bsa29I ATCGAT 1 cut(s) 730
BsaAI YACGTR 2 cut(s) 155, 1028
BsaBI GATNNNNATC 1 cut(s) 2448
BsaHI GRCGYC 2 cut(s) 97, 2273
BsaI GGTCTC 1 cut(s) 1171
BsaJI CCNNGG 4 cut(s) 307, 750, 1929, 2332
Bsc4I CCNNNNNNNGG 8 cut(s) 662, 751, 946, 1935, 2029, 2156, 2345, 2573
Bse118I RCCGGY 1 cut(s) 2622
Bse1I ACTGG 6 cut(s) 391, 883, 960, 1152, 1167, 2309
Bse3DI GCAATG 3 cut(s) 841, 2101, 2191
Bse8I GATNNNNATC 1 cut(s) 2448
BseBI CCWGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
BseCI ATCGAT 1 cut(s) 730
BseDI CCNNGG 4 cut(s) 307, 750, 1929, 2332
BseGI GGATG 9 cut(s) 302, 493, 715, 956, 1939, 1945, 2260, 2431, 2449
BseJI GATNNNNATC 1 cut(s) 2448
BseLI CCNNNNNNNGG 8 cut(s) 662, 751, 946, 1935, 2029, 2156, 2345, 2573
BseMI GCAATG 3 cut(s) 841, 2101, 2191
BseMII CTCAG 7 cut(s) 72, 141, 444, 1106, 1551, 1971, 2685
BseNI ACTGG 6 cut(s) 391, 883, 960, 1152, 1167, 2309
BseRI GAGGAG 1 cut(s) 1124
BseSI GKGCMC 2 cut(s) 58, 2588
BseYI CCCAGC 2 cut(s) 1315, 2022
BsgI GTGCAG 2 cut(s) 834, 852
Bsh1236I CGCG 2 cut(s) 1690, 2526
BshFI GGCC 7 cut(s) 331, 749, 939, 1671, 1990, 2586, 2727
BshNI GGYRCC 1 cut(s) 2455
BshVI ATCGAT 1 cut(s) 730
BsiHKCI CYCGRG 1 cut(s) 1642
BsiSI CCGG 1 cut(s) 2623
BslFI GGGAC 2 cut(s) 333, 605
BslI CCNNNNNNNGG 8 cut(s) 662, 751, 946, 1935, 2029, 2156, 2345, 2573
BsmAI GTCTC 3 cut(s) 1034, 1171, 1511
BsmFI GGGAC 2 cut(s) 333, 605
BsmI GAATGC 5 cut(s) 1014, 1350, 1453, 1651, 2480
BsnI GGCC 7 cut(s) 331, 749, 939, 1671, 1990, 2586, 2727
Bso31I GGTCTC 1 cut(s) 1171
BsoBI CYCGRG 1 cut(s) 1642
Bsp120I GGGCCC 1 cut(s) 2584
Bsp1286I GDGCHC 2 cut(s) 58, 2588
Bsp1407I TGTACA 1 cut(s) 1234
Bsp143I GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
Bsp19I CCATGG 1 cut(s) 1929
BspACI CCGC 8 cut(s) 244, 535, 1709, 2252, 2289, 2339, 2364, 2728
BspANI GGCC 7 cut(s) 331, 749, 939, 1671, 1990, 2586, 2727
BspCNI CTCAG 7 cut(s) 73, 140, 445, 1107, 1552, 1972, 2686
BspDI ATCGAT 1 cut(s) 730
BspFNI CGCG 2 cut(s) 1690, 2526
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 9 cut(s) 321, 668, 1323, 1703, 2034, 2136, 2196, 2457, 2586
BspMAI CTGCAG 1 cut(s) 2679
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 5 cut(s) 125, 1316, 1329, 1696, 1709
BspT107I GGYRCC 1 cut(s) 2455
BspTI CTTAAG 1 cut(s) 2545
BspTNI GGTCTC 1 cut(s) 1171
BsrDI GCAATG 3 cut(s) 841, 2101, 2191
BsrFI RCCGGY 1 cut(s) 2622
BsrGI TGTACA 1 cut(s) 1234
BsrI ACTGG 6 cut(s) 391, 883, 960, 1152, 1167, 2309
BssAI RCCGGY 1 cut(s) 2622
BssECI CCNNGG 4 cut(s) 307, 750, 1929, 2332
BssMI GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
BssNI GRCGYC 2 cut(s) 97, 2273
BssSI CACGAG 2 cut(s) 117, 603
BssT1I CCWWGG 3 cut(s) 307, 1929, 2332
Bst2BI CACGAG 2 cut(s) 117, 603
Bst2UI CCWGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
Bst4CI ACNGT 7 cut(s) 579, 724, 828, 1186, 1422, 2557, 2712
Bst6I CTCTTC 2 cut(s) 1101, 1979
BstACI GRCGYC 2 cut(s) 97, 2273
BstAFI CTTAAG 1 cut(s) 2545
BstAPI GCANNNNNTGC 2 cut(s) 911, 1268
BstAUI TGTACA 1 cut(s) 1234
BstBAI YACGTR 2 cut(s) 155, 1028
BstDEI CTNAG 8 cut(s) 81, 127, 453, 1115, 1297, 1560, 1980, 2694
BstDSI CCRYGG 2 cut(s) 750, 1929
BstEII GGTNACC 1 cut(s) 554
BstENI CCTNNNNNAGG 1 cut(s) 944
BstF5I GGATG 9 cut(s) 302, 493, 715, 956, 1939, 1945, 2260, 2431, 2449
BstFNI CGCG 2 cut(s) 1690, 2526
BstHHI GCGC 2 cut(s) 261, 1690
BstKTI GATC 9 cut(s) 9, 133, 337, 417, 956, 1324, 1704, 1747, 2650
BstMAI GTCTC 3 cut(s) 1034, 1171, 1511
BstMBI GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
BstNI CCWGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
BstNSI RCATGY 2 cut(s) 1295, 1380
BstPI GGTNACC 1 cut(s) 554
BstSCI CCNGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
BstSFI CTRYAG 2 cut(s) 288, 2675
BstSLI GKGCMC 2 cut(s) 58, 2588
BstUI CGCG 2 cut(s) 1690, 2526
BstV2I GAAGAC 2 cut(s) 1267, 2276
BstX2I RGATCY 3 cut(s) 130, 1321, 1701
BstXI CCANNNNNNTGG 1 cut(s) 1897
BstYI RGATCY 3 cut(s) 130, 1321, 1701
Bsu15I ATCGAT 1 cut(s) 730
BsuRI GGCC 7 cut(s) 331, 749, 939, 1671, 1990, 2586, 2727
BsuTUI ATCGAT 1 cut(s) 730
BtgI CCRYGG 2 cut(s) 750, 1929
BtgZI GCGATG 1 cut(s) 2507
BtsCI GGATG 9 cut(s) 302, 493, 715, 956, 1939, 1945, 2260, 2431, 2449
BtsI GCAGTG 2 cut(s) 1078, 1408
BtsIMutI CAGTG 5 cut(s) 277, 1078, 1408, 2417, 2562
BveI ACCTGC 1 cut(s) 919
CaiI CAGNNNCTG 1 cut(s) 2204
CciI TCATGA 1 cut(s) 397
CfoI GCGC 2 cut(s) 261, 1690
Cfr10I RCCGGY 1 cut(s) 2622
Cfr13I GGNCC 7 cut(s) 184, 320, 1904, 2243, 2336, 2584, 2585
ClaI ATCGAT 1 cut(s) 730
CseI GACGC 2 cut(s) 86, 1679
Csp6I GTAC 4 cut(s) 1029, 1235, 2105, 2163
CviQI GTAC 4 cut(s) 1029, 1235, 2105, 2163
DdeI CTNAG 8 cut(s) 81, 127, 453, 1115, 1297, 1560, 1980, 2694
DpnI GATC 9 cut(s) 8, 132, 336, 416, 955, 1323, 1703, 1746, 2649
DpnII GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
DraI TTTAAA 3 cut(s) 364, 1966, 2041
DrdI GACNNNNNNGTC 1 cut(s) 2334
DriI GACNNNNNGTC 1 cut(s) 2714
DseDI GACNNNNNNGTC 1 cut(s) 2334
EaeI YGGCCR 2 cut(s) 747, 1669
Eam1104I CTCTTC 2 cut(s) 1101, 1979
Eam1105I GACNNNNNGTC 1 cut(s) 2714
EarI CTCTTC 2 cut(s) 1101, 1979
EciI GGCGGA 1 cut(s) 2328
Eco130I CCWWGG 3 cut(s) 307, 1929, 2332
Eco147I AGGCCT 2 cut(s) 939, 1990
Eco24I GRGCYC 1 cut(s) 2588
Eco31I GGTCTC 1 cut(s) 1171
Eco32I GATATC 1 cut(s) 525
Eco47I GGWCC 5 cut(s) 184, 320, 1904, 2243, 2336
Eco57I CTGAAG 1 cut(s) 253
Eco88I CYCGRG 1 cut(s) 1642
Eco91I GGTNACC 1 cut(s) 554
EcoNI CCTNNNNNAGG 1 cut(s) 944
EcoO109I RGGNCCY 2 cut(s) 320, 2584
EcoO65I GGTNACC 1 cut(s) 554
EcoRI GAATTC 1 cut(s) 2630
EcoRII CCWGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
EcoRV GATATC 1 cut(s) 525
EcoT14I CCWWGG 3 cut(s) 307, 1929, 2332
EcoT22I ATGCAT 3 cut(s) 193, 1264, 2482
EcoT38I GRGCYC 1 cut(s) 2588
ErhI CCWWGG 3 cut(s) 307, 1929, 2332
FalI AAGNNNNNCTT 2 cut(s) 924, 956
FaqI GGGAC 2 cut(s) 333, 605
FauI CCCGC 2 cut(s) 2296, 2371
FauNDI CATATG 2 cut(s) 1222, 2637
FbaI TGATCA 2 cut(s) 6, 1744
FblI GTMKAC 1 cut(s) 2317
FokI GGATG 9 cut(s) 289, 500, 722, 943, 1946, 1952, 2267, 2438, 2456
FriOI GRGCYC 1 cut(s) 2588
FspBI CTAG 2 cut(s) 1032, 2166
FspI TGCGCA 1 cut(s) 260
GlaI GCGC 2 cut(s) 260, 1689
GsaI CCCAGC 2 cut(s) 1319, 2026
GsuI CTGGAG 3 cut(s) 1058, 1119, 2181
HaeIII GGCC 7 cut(s) 331, 749, 939, 1671, 1990, 2586, 2727
HapII CCGG 1 cut(s) 2623
HgaI GACGC 2 cut(s) 86, 1679
HhaI GCGC 2 cut(s) 261, 1690
Hin1I GRCGYC 2 cut(s) 97, 2273
Hin6I GCGC 2 cut(s) 259, 1688
HinP1I GCGC 2 cut(s) 259, 1688
HincII GTYRAC 2 cut(s) 1926, 2318
HindII GTYRAC 2 cut(s) 1926, 2318
HindIII AAGCTT 1 cut(s) 981
HpaII CCGG 1 cut(s) 2623
HphI GGTGA 6 cut(s) 566, 730, 1328, 1826, 2420, 2651
Hpy166II GTNNAC 5 cut(s) 1926, 2318, 2377, 2560, 2579
Hpy8I GTNNAC 5 cut(s) 1926, 2318, 2377, 2560, 2579
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 5 cut(s) 310, 1205, 1544, 1669, 1812
HpyCH4III ACNGT 7 cut(s) 579, 724, 828, 1186, 1422, 2557, 2712
HpyCH4IV ACGT 4 cut(s) 61, 154, 1027, 2273
HpyF3I CTNAG 8 cut(s) 81, 127, 453, 1115, 1297, 1560, 1980, 2694
HpySE526I ACGT 4 cut(s) 61, 154, 1027, 2273
Hsp92I GRCGYC 2 cut(s) 97, 2273
HspAI GCGC 2 cut(s) 259, 1688
KroI GCCGGC 1 cut(s) 2622
KroNI GCCGGC 1 cut(s) 2624
Ksp22I TGATCA 2 cut(s) 6, 1744
Kzo9I GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
LmnI GCTCC 4 cut(s) 49, 1100, 1111, 2200
LweI GCATC 5 cut(s) 653, 1120, 1295, 1396, 2243
MaeI CTAG 2 cut(s) 1032, 2166
MaeII ACGT 4 cut(s) 61, 154, 1027, 2273
MaeIII GTNAC 7 cut(s) 231, 554, 626, 718, 815, 1334, 1341
MalI GATC 9 cut(s) 8, 132, 336, 416, 955, 1323, 1703, 1746, 2649
MboI GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
MflI RGATCY 3 cut(s) 130, 1321, 1701
MhlI GDGCHC 2 cut(s) 58, 2588
MlsI TGGCCA 1 cut(s) 1671
MluNI TGGCCA 1 cut(s) 1671
MlyI GAGTC 4 cut(s) 587, 1126, 2390, 2408
MmeI TCCRAC 5 cut(s) 66, 80, 1349, 2167, 2421
Mox20I TGGCCA 1 cut(s) 1671
Mph1103I ATGCAT 3 cut(s) 193, 1264, 2482
MroNI GCCGGC 1 cut(s) 2622
MscI TGGCCA 1 cut(s) 1671
MslI CAYNNNNRTG 1 cut(s) 270
Msp20I TGGCCA 1 cut(s) 1671
MspA1I CMGCKG 5 cut(s) 537, 1397, 2291, 2436, 2674
MspCI CTTAAG 1 cut(s) 2545
MspI CCGG 1 cut(s) 2623
MspR9I CCNGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
Mva1269I GAATGC 5 cut(s) 1014, 1350, 1453, 1651, 2480
MvaI CCWGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
MvnI CGCG 2 cut(s) 1690, 2526
NaeI GCCGGC 1 cut(s) 2624
NcoI CCATGG 1 cut(s) 1929
NdeI CATATG 2 cut(s) 1222, 2637
NdeII GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
NgoMIV GCCGGC 1 cut(s) 2622
NlaIV GGNNCC 9 cut(s) 321, 668, 1323, 1703, 2034, 2136, 2196, 2457, 2586
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 3 cut(s) 554, 718, 1334
NsbI TGCGCA 1 cut(s) 260
NsiI ATGCAT 3 cut(s) 193, 1264, 2482
NspI RCATGY 2 cut(s) 1295, 1380
OliI CACNNNNGTG 1 cut(s) 270
PaeR7I CTCGAG 1 cut(s) 1642
PagI TCATGA 1 cut(s) 397
PceI AGGCCT 2 cut(s) 939, 1990
PcsI WCGNNNNNNNCGW 3 cut(s) 334, 736, 1000
PctI GAATGC 5 cut(s) 1014, 1350, 1453, 1651, 2480
PdiI GCCGGC 1 cut(s) 2624
PfeI GAWTC 6 cut(s) 458, 805, 1564, 1602, 1766, 2413
PflMI CCANNNNNTGG 2 cut(s) 662, 2029
PleI GAGTC 4 cut(s) 587, 1125, 2389, 2407
PpsI GAGTC 4 cut(s) 587, 1125, 2389, 2407
Ppu21I YACGTR 2 cut(s) 155, 1028
PpuMI RGGWCCY 1 cut(s) 320
PsiI TTATAA 1 cut(s) 2391
Psp1406I AACGTT 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 320
Psp6I CCWGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
PspEI GGTNACC 1 cut(s) 554
PspFI CCCAGC 2 cut(s) 1315, 2022
PspGI CCWGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
PspN4I GGNNCC 9 cut(s) 321, 668, 1323, 1703, 2034, 2136, 2196, 2457, 2586
PspOMI GGGCCC 1 cut(s) 2584
PspPI GGNCC 7 cut(s) 184, 320, 1904, 2243, 2336, 2584, 2585
PspPPI RGGWCCY 1 cut(s) 320
PstI CTGCAG 1 cut(s) 2679
PstNI CAGNNNCTG 1 cut(s) 2204
PsuI RGATCY 3 cut(s) 130, 1321, 1701
PvuII CAGCTG 3 cut(s) 1397, 2436, 2674
RsaI GTAC 4 cut(s) 1030, 1236, 2106, 2164
RsaNI GTAC 4 cut(s) 1029, 1235, 2105, 2163
RseI CAYNNNNRTG 1 cut(s) 270
SalI GTCGAC 1 cut(s) 2316
Sau3AI GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
Sau96I GGNCC 7 cut(s) 184, 320, 1904, 2243, 2336, 2584, 2585
ScaI AGTACT 2 cut(s) 2106, 2164
SchI GAGTC 4 cut(s) 587, 1126, 2390, 2408
ScrFI CCNGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
SduI GDGCHC 2 cut(s) 58, 2588
SfaNI GCATC 5 cut(s) 653, 1120, 1295, 1396, 2243
SfcI CTRYAG 2 cut(s) 288, 2675
Sfr274I CTCGAG 1 cut(s) 1642
SinI GGWCC 5 cut(s) 184, 320, 1904, 2243, 2336
SlaI CTCGAG 1 cut(s) 1642
SmiI ATTTAAAT 1 cut(s) 2041
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 4 cut(s) 179, 622, 1642, 2545
SmoI CTYRAG 4 cut(s) 179, 622, 1642, 2545
SpeI ACTAGT 1 cut(s) 2165
SseBI AGGCCT 2 cut(s) 939, 1990
SsiI CCGC 8 cut(s) 244, 535, 1709, 2252, 2289, 2339, 2364, 2728
SspI AATATT 1 cut(s) 1632
SspMI CTAG 2 cut(s) 1032, 2166
StuI AGGCCT 2 cut(s) 939, 1990
StyD4I CCNGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
StyI CCWWGG 3 cut(s) 307, 1929, 2332
SwaI ATTTAAAT 1 cut(s) 2041
TaaI ACNGT 7 cut(s) 579, 724, 828, 1186, 1422, 2557, 2712
TaiI ACGT 4 cut(s) 64, 157, 1030, 2276
TaqI TCGA 8 cut(s) 69, 469, 730, 1159, 1278, 1643, 2317, 2643
TatI WGTACW 3 cut(s) 1234, 2104, 2162
TauI GCSGC 2 cut(s) 1712, 2730
TfiI GAWTC 6 cut(s) 458, 805, 1564, 1602, 1766, 2413
TscAI CASTG 5 cut(s) 277, 1078, 1408, 2424, 2562
TseFI GTSAC 3 cut(s) 554, 718, 1334
Tsp45I GTSAC 3 cut(s) 554, 718, 1334
TspDTI ATGAA 9 cut(s) 180, 524, 1178, 1428, 1560, 1594, 1823, 1965, 2679
TspGWI ACGGA 1 cut(s) 992
TspRI CASTG 5 cut(s) 277, 1078, 1408, 2424, 2562
Van91I CCANNNNNTGG 2 cut(s) 662, 2029
Vha464I CTTAAG 1 cut(s) 2545
VpaK11BI GGWCC 5 cut(s) 184, 320, 1904, 2243, 2336
XagI CCTNNNNNAGG 1 cut(s) 944
XapI RAATTY 2 cut(s) 1781, 2630
XceI RCATGY 2 cut(s) 1295, 1380
XcmI CCANNNNNNNNNTGG 1 cut(s) 1861
XhoI CTCGAG 1 cut(s) 1642
XmiI GTMKAC 1 cut(s) 2317
XspI CTAG 2 cut(s) 1032, 2166
ZraI GACGTC 1 cut(s) 2274
ZrmI AGTACT 2 cut(s) 2106, 2164
Zsp2I ATGCAT 3 cut(s) 193, 1264, 2482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.