Rroxscaffold_1G00030290

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008095
Physical Location & Seq
Reverse (-)
39783483 .. 39790395
6913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00030290.1

Sequence Viewer

Length: 2646 bp
ATGATGATCACAAAATTAGATAAAATGTCTGCTCCTCCATCATGGGAGCGTGCCCAACGTTTATTCGACAAGATTACTGAGTTGGAGAGTAGGCGTCGGAGAGAGCAGCACAATGTTGAGTATGCTTTGTGGCAGTTACATTACAAGCGGATTGACGAATTGCGCACGCACTTCAGTGCTGCTATGGCTTCTACAGGTTCAAACTCATCCCAAGGTTTGAAGGGACCTACTCGGCCTGATCGTATTACAAAAATAAGATTGCAGTTTAAAACATTCCTTTCAGAAGCGACTGGATTTTATCATGATTTAATAGTGAAGATCAGAGCAAAGTATGGGCTTCCTCTTGGCTATTTCTCTGAGGATTCTGAAAATCGAATTGTTATGGATAAGGATGGAAAGAAATCTGCTGATATGAAGAAAGGTTTGATATCATGCCACCGCTGTTTGATTTACTTGGGTGACCTTGCACGCTACAAAGGACTGTATGGGGAGGGGGACTCCAAAACTCGTGAGTATGCTGCTGCCTCAAGTTACTACTTGCAAGCTGCATCTTGTTTGCCATCAAGTGGGAACCCTCATCATCAGCTTGCTATATTGGCTTCTTATTCGGGGGATGAGTTGGTGACTGTTTATCGATATTTTCGTAGCCTGGCCGTGGATAATCCCTTTTCAACTGCAAGGGATAACTTGATTGTGGCATTTGAGAAGAATCGTCAGAGTTACTCGCAACTGTCTGGCAATGCTAATGCATCTGCACTCAAAGAGTTGCCTGCACGATTGACTGGCAAAGGTAGAGGGAAAGGGGAAGCAATACCTGCTAAAGATAACAACACAGAAGCAGGCCTTGTTAAGGAAAGATCATCCAGTAATCAGGAAAAATACAAAGCTTTTTCAATTCGTTTTGTCCGTCTAAATGGCATTCTTTTTACACGTACTAGCCTGGAGACATTTGCAGAAGTTCTCTCTGTTGTAAGCAGTGGTTTATATGAGCTTCTATCTTCTGGAGCAGAAGAGGAGCTGAGTCTTGGTGCTGATGCTGTTGAGAATGGACTGGTCATTGTTCGACTGGTCTCTATACTGATATTCACAGTTCATAATGTGAAAAAGGAAAGTGAAGGTCAGTCATATGCAGAAATTGTACAGCGTGCTGTTCTGCTGCAAAATGCATTTACTGCTGTCTTTGAGTTGATGGGGCATGTCTTAGAGAGATGCGTGAAGCTGGGGAATCCAACTTCAAGTCACCTGTTACCAGGCATTCTGGTTTTTGTAGAATGGTTGGCATGTTGTCCTGATGTAGCAGCTGGCAGTGATGCAGAAGAAAAACAGTCTTCTGTTAGAGCAAAGTTCTGGAATGCTTGCATACACTTGTTGAATAATCTCTTATCATCTGTTCCGATGTCCATAGATGATGATGGAGATGAGACTTGCTTTAATAATATGAGCAGATATGAAGAAGGGGAAACTGAGAATCGCCTTGCTTTGTGGGAGGATTTTGAGTTGAGAGGATTCATGCCACTTCTTCCAGCACAGACAATATTGGATTTCTCGAGGAAGCATTCTTTTGGAAGTGATGGCCAGAAGGAAAAAGGTGCGCGTGTTAAAAGGATCCTGGCGGCAGGCAAGGCTTTAGCAAATGTGGTCAAGGTTGATCAAAAAGCCATTTATTTTGATTCCCAGACAAAGAAATTTGTCATCGGTGTTGAGCCTTCAATGAATGGCGATTATATGCCTACCTCCTTTTTAGGCATGGCTAATTCCAATGATAATCTGGAAGAAAATCAAGTGGAGGACACCAAAAATCTGGGAGGTCCATTTCAAAAGCCAGAGTTGACCATGGATGGGGATGAGGAAGATGAAGTAATAGTTTTTAAACCAATAGTGCCTGAGAAGAGGCCTGATGTGGTAGGCACTACATGGGCTATCCCCCAGCATTTGGAACCATTTAAATCAGAAGAAATATCTCTTGCTAACAACCTAAAGAGTTTGGGATTTATGGGCAATGGGCAAGTACTGAAATCTGACCATGTATCAAGTTCGGTTCCATTCCAACAGCCTGTTAATGGTAGTACTAGCAGTATGTTTTACAGCCATGCAAAGGGTCCAGAAGCTGTGTTGCCATTTAAGGTTGATACTATTGCATCTTCTGGACCATTTGCGGATGGTTTGACATTGAAGACGTCATCAGCTTTGCCCGCTGCTATCCGAAAAAATCCAGTTAGTCGACCTGTTAGACACCTTGGTCCGCCACCTGGTTTTAGCCATGTTCCCGCTAAACAAGTGAACGAGTCTATTTATAATTCAGAGTCAATGGGTGAGAATCCACTGATGGATGATTACAGCTGGTTGGATGGGTATCAGGTGCCATCTTCAACTAAAGGAAATGCATTCAGCAGTTCCATCAACTACTCATCTCATTCAAATCTCCATCGCGTTCCTAATGGGAATGGCTTAAGTGGAACAGTGAACTTTCCCTTTCCTGGTAAACAGGGCCCATCTATGCCATTCCAAGCAGAAAACCAGAAAAGCCGGCAAGAATTCCATATGCTCGATGATCTTAAACTTCACCATGAAATGCAGCTGCAGCAGCAGCAACAACCTGAGCAATATCAAGGACAGTCTGTCTGGACAGGCCGCTATTTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

881

Amino Acids

97.81

Weight (kDa)

6.46

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 39 - 162 2.4e-17 Telomerase activating protein Est1
EST1_DNA_bind PF10373 175 - 508 3.8e-64 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2295
AasI GACNNNNNNGTC 1 cut(s) 2238
AatII GACGTC 1 cut(s) 2180
Acc16I TGCGCA 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 823
AccB1I GGYRCC 1 cut(s) 2359
AccB7I CCANNNNNTGG 2 cut(s) 566, 1933
AccI GTMKAC 1 cut(s) 2221
AccII CGCG 2 cut(s) 1594, 2430
AciI CCGC 8 cut(s) 148, 439, 1613, 2156, 2193, 2243, 2268, 2632
AclI AACGTT 1 cut(s) 58
AclWI GGATC 2 cut(s) 1600, 1613
AcoI YGGCCR 2 cut(s) 651, 1573
AcsI RAATTY 2 cut(s) 1685, 2534
AcuI CTGAAG 1 cut(s) 157
AcyI GRCGYC 2 cut(s) 94, 2177
AfaI GTAC 4 cut(s) 934, 1140, 2010, 2068
AfiI CCNNNNNNNGG 9 cut(s) 566, 655, 850, 1839, 1933, 2060, 2095, 2249, 2477
AflII CTTAAG 1 cut(s) 2449
AflIII ACRYGT 1 cut(s) 929
AhdI GACNNNNNGTC 1 cut(s) 2618
AjnI CCWGG 6 cut(s) 648, 939, 1249, 1608, 2248, 2476
AleI CACNNNNGTG 1 cut(s) 174
Alw26I GTCTC 3 cut(s) 938, 1075, 1415
AlwI GGATC 2 cut(s) 1600, 1613
AlwNI CAGNNNCTG 1 cut(s) 2108
Ama87I CYCGRG 1 cut(s) 1546
AoxI GGCC 7 cut(s) 233, 651, 841, 1573, 1892, 2488, 2629
ApaI GGGCCC 1 cut(s) 2492
ApoI RAATTY 2 cut(s) 1685, 2534
AspLEI GCGC 2 cut(s) 165, 1594
AspS9I GGNCC 7 cut(s) 224, 1808, 2099, 2147, 2240, 2488, 2489
AsuHPI GGTGA 5 cut(s) 470, 634, 1232, 2324, 2555
AvaI CYCGRG 1 cut(s) 1546
AvaII GGWCC 5 cut(s) 224, 1808, 2099, 2147, 2240
BaeGI GKGCMC 2 cut(s) 55, 2492
BalI TGGCCA 1 cut(s) 1575
BamHI GGATCC 1 cut(s) 1605
BanI GGYRCC 1 cut(s) 2359
BanII GRGCYC 1 cut(s) 2492
BauI CACGAG 1 cut(s) 507
BbsI GAAGAC 2 cut(s) 1320, 2180
BceAI ACGGC 1 cut(s) 638
BciT130I CCWGG 6 cut(s) 650, 941, 1251, 1610, 2250, 2478
BclI TGATCA 2 cut(s) 6, 1648
BcoDI GTCTC 3 cut(s) 938, 1075, 1415
BfaI CTAG 2 cut(s) 936, 2070
BfmI CTRYAG 2 cut(s) 192, 2579
BfrI CTTAAG 1 cut(s) 2449
BfuAI ACCTGC 1 cut(s) 823
BmcAI AGTACT 2 cut(s) 2010, 2068
Bme1390I CCNGG 6 cut(s) 650, 941, 1251, 1610, 2250, 2478
Bme18I GGWCC 5 cut(s) 224, 1808, 2099, 2147, 2240
BmeRI GACNNNNNGTC 1 cut(s) 2618
BmeT110I CYCGRG 1 cut(s) 1546
BmgT120I GGNCC 7 cut(s) 224, 1808, 2099, 2147, 2240, 2488, 2489
BmiI GGNNCC 8 cut(s) 225, 572, 1607, 1938, 2040, 2100, 2361, 2490
BmrFI CCNGG 6 cut(s) 650, 941, 1251, 1610, 2250, 2478
BmsI GCATC 6 cut(s) 557, 758, 1024, 1199, 1300, 2147
BpiI GAAGAC 2 cut(s) 1320, 2180
BplI GAGNNNNNCTC 2 cut(s) 482, 514
BpmI CTGGAG 2 cut(s) 962, 1023
Bpu10I CCTNAGC 1 cut(s) 2598
BpuEI CTTGAG 1 cut(s) 511
Bsa29I ATCGAT 1 cut(s) 634
BsaAI YACGTR 1 cut(s) 932
BsaBI GATNNNNATC 1 cut(s) 2352
BsaHI GRCGYC 2 cut(s) 94, 2177
BsaI GGTCTC 1 cut(s) 1075
BsaJI CCNNGG 4 cut(s) 211, 654, 1833, 2236
Bsc4I CCNNNNNNNGG 9 cut(s) 566, 655, 850, 1839, 1933, 2060, 2095, 2249, 2477
Bse118I RCCGGY 1 cut(s) 2526
Bse1I ACTGG 6 cut(s) 295, 787, 864, 1056, 1071, 2213
Bse3DI GCAATG 2 cut(s) 745, 2005
Bse8I GATNNNNATC 1 cut(s) 2352
BseBI CCWGG 6 cut(s) 650, 941, 1251, 1610, 2250, 2478
BseCI ATCGAT 1 cut(s) 634
BseDI CCNNGG 4 cut(s) 211, 654, 1833, 2236
BseGI GGATG 9 cut(s) 206, 397, 619, 860, 1843, 1849, 2164, 2335, 2353
BseJI GATNNNNATC 1 cut(s) 2352
BseLI CCNNNNNNNGG 9 cut(s) 566, 655, 850, 1839, 1933, 2060, 2095, 2249, 2477
BseMI GCAATG 2 cut(s) 745, 2005
BseMII CTCAG 6 cut(s) 69, 348, 1010, 1455, 1875, 2589
BseNI ACTGG 6 cut(s) 295, 787, 864, 1056, 1071, 2213
BseRI GAGGAG 2 cut(s) 24, 1028
BseSI GKGCMC 2 cut(s) 55, 2492
BseYI CCCAGC 2 cut(s) 1219, 1926
BsgI GTGCAG 2 cut(s) 738, 756
Bsh1236I CGCG 2 cut(s) 1594, 2430
BshFI GGCC 7 cut(s) 235, 653, 843, 1575, 1894, 2490, 2631
BshNI GGYRCC 1 cut(s) 2359
BshVI ATCGAT 1 cut(s) 634
BsiHKCI CYCGRG 1 cut(s) 1546
BsiSI CCGG 1 cut(s) 2527
BslFI GGGAC 2 cut(s) 237, 509
BslI CCNNNNNNNGG 9 cut(s) 566, 655, 850, 1839, 1933, 2060, 2095, 2249, 2477
BsmAI GTCTC 3 cut(s) 938, 1075, 1415
BsmFI GGGAC 2 cut(s) 237, 509
BsmI GAATGC 5 cut(s) 918, 1254, 1357, 1555, 2384
BsnI GGCC 7 cut(s) 235, 653, 843, 1575, 1894, 2490, 2631
Bso31I GGTCTC 1 cut(s) 1075
BsoBI CYCGRG 1 cut(s) 1546
Bsp120I GGGCCC 1 cut(s) 2488
Bsp1286I GDGCHC 2 cut(s) 55, 2492
Bsp1407I TGTACA 1 cut(s) 1138
Bsp143I GATC 7 cut(s) 6, 238, 318, 857, 1605, 1648, 2551
Bsp19I CCATGG 1 cut(s) 1833
BspACI CCGC 8 cut(s) 148, 439, 1613, 2156, 2193, 2243, 2268, 2632
BspANI GGCC 7 cut(s) 235, 653, 843, 1575, 1894, 2490, 2631
BspCNI CTCAG 6 cut(s) 70, 349, 1011, 1456, 1876, 2590
BspDI ATCGAT 1 cut(s) 634
BspFNI CGCG 2 cut(s) 1594, 2430
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 8 cut(s) 225, 572, 1607, 1938, 2040, 2100, 2361, 2490
BspMAI CTGCAG 1 cut(s) 2583
BspMI ACCTGC 1 cut(s) 823
BspPI GGATC 2 cut(s) 1600, 1613
BspT107I GGYRCC 1 cut(s) 2359
BspTI CTTAAG 1 cut(s) 2449
BspTNI GGTCTC 1 cut(s) 1075
BsrDI GCAATG 2 cut(s) 745, 2005
BsrFI RCCGGY 1 cut(s) 2526
BsrGI TGTACA 1 cut(s) 1138
BsrI ACTGG 6 cut(s) 295, 787, 864, 1056, 1071, 2213
BssAI RCCGGY 1 cut(s) 2526
BssECI CCNNGG 4 cut(s) 211, 654, 1833, 2236
BssMI GATC 7 cut(s) 6, 238, 318, 857, 1605, 1648, 2551
BssNI GRCGYC 2 cut(s) 94, 2177
BssSI CACGAG 1 cut(s) 507
BssT1I CCWWGG 3 cut(s) 211, 1833, 2236
Bst2BI CACGAG 1 cut(s) 507
Bst2UI CCWGG 6 cut(s) 650, 941, 1251, 1610, 2250, 2478
Bst4CI ACNGT 7 cut(s) 483, 628, 732, 1090, 1326, 2461, 2616
Bst6I CTCTTC 2 cut(s) 1005, 1883
BstACI GRCGYC 2 cut(s) 94, 2177
BstAFI CTTAAG 1 cut(s) 2449
BstAPI GCANNNNNTGC 2 cut(s) 815, 1172
BstAUI TGTACA 1 cut(s) 1138
BstBAI YACGTR 1 cut(s) 932
BstDEI CTNAG 7 cut(s) 78, 357, 1019, 1201, 1464, 1884, 2598
BstDSI CCRYGG 2 cut(s) 654, 1833
BstEII GGTNACC 1 cut(s) 458
BstENI CCTNNNNNAGG 1 cut(s) 848
BstF5I GGATG 9 cut(s) 206, 397, 619, 860, 1843, 1849, 2164, 2335, 2353
BstFNI CGCG 2 cut(s) 1594, 2430
BstHHI GCGC 2 cut(s) 165, 1594
BstKTI GATC 7 cut(s) 9, 241, 321, 860, 1608, 1651, 2554
BstMAI GTCTC 3 cut(s) 938, 1075, 1415
BstMBI GATC 7 cut(s) 6, 238, 318, 857, 1605, 1648, 2551
BstMWI GCNNNNNNNGC 9 cut(s) 185, 596, 815, 1172, 1622, 2192, 2581, 2584, 2587
BstNI CCWGG 6 cut(s) 650, 941, 1251, 1610, 2250, 2478
BstNSI RCATGY 2 cut(s) 1199, 1284
BstPI GGTNACC 1 cut(s) 458
BstSCI CCNGG 6 cut(s) 648, 939, 1249, 1608, 2248, 2476
BstSFI CTRYAG 2 cut(s) 192, 2579
BstSLI GKGCMC 2 cut(s) 55, 2492
BstUI CGCG 2 cut(s) 1594, 2430
BstV2I GAAGAC 2 cut(s) 1320, 2180
BstX2I RGATCY 1 cut(s) 1605
BstXI CCANNNNNNTGG 1 cut(s) 1801
BstYI RGATCY 1 cut(s) 1605
Bsu15I ATCGAT 1 cut(s) 634
BsuRI GGCC 7 cut(s) 235, 653, 843, 1575, 1894, 2490, 2631
BsuTUI ATCGAT 1 cut(s) 634
BtgI CCRYGG 2 cut(s) 654, 1833
BtgZI GCGATG 1 cut(s) 2411
BtsCI GGATG 9 cut(s) 206, 397, 619, 860, 1843, 1849, 2164, 2335, 2353
BtsI GCAGTG 2 cut(s) 982, 1312
BtsIMutI CAGTG 5 cut(s) 181, 982, 1312, 2321, 2466
BveI ACCTGC 1 cut(s) 823
CaiI CAGNNNCTG 1 cut(s) 2108
CciI TCATGA 1 cut(s) 301
CfoI GCGC 2 cut(s) 165, 1594
Cfr10I RCCGGY 1 cut(s) 2526
Cfr13I GGNCC 7 cut(s) 224, 1808, 2099, 2147, 2240, 2488, 2489
ClaI ATCGAT 1 cut(s) 634
CseI GACGC 1 cut(s) 83
CsiI ACCWGGT 1 cut(s) 2248
Csp6I GTAC 4 cut(s) 933, 1139, 2009, 2067
CviQI GTAC 4 cut(s) 933, 1139, 2009, 2067
DdeI CTNAG 7 cut(s) 78, 357, 1019, 1201, 1464, 1884, 2598
DpnI GATC 7 cut(s) 8, 240, 320, 859, 1607, 1650, 2553
DpnII GATC 7 cut(s) 6, 238, 318, 857, 1605, 1648, 2551
DraI TTTAAA 3 cut(s) 268, 1870, 1945
DrdI GACNNNNNNGTC 1 cut(s) 2238
DriI GACNNNNNGTC 1 cut(s) 2618
DseDI GACNNNNNNGTC 1 cut(s) 2238
EaeI YGGCCR 2 cut(s) 651, 1573
Eam1104I CTCTTC 2 cut(s) 1005, 1883
Eam1105I GACNNNNNGTC 1 cut(s) 2618
EarI CTCTTC 2 cut(s) 1005, 1883
EciI GGCGGA 1 cut(s) 2232
Eco130I CCWWGG 3 cut(s) 211, 1833, 2236
Eco147I AGGCCT 2 cut(s) 843, 1894
Eco24I GRGCYC 1 cut(s) 2492
Eco31I GGTCTC 1 cut(s) 1075
Eco32I GATATC 1 cut(s) 429
Eco47I GGWCC 5 cut(s) 224, 1808, 2099, 2147, 2240
Eco57I CTGAAG 1 cut(s) 157
Eco88I CYCGRG 1 cut(s) 1546
Eco91I GGTNACC 1 cut(s) 458
EcoNI CCTNNNNNAGG 1 cut(s) 848
EcoO109I RGGNCCY 2 cut(s) 224, 2488
EcoO65I GGTNACC 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 2534
EcoRII CCWGG 6 cut(s) 648, 939, 1249, 1608, 2248, 2476
EcoRV GATATC 1 cut(s) 429
EcoT14I CCWWGG 3 cut(s) 211, 1833, 2236
EcoT22I ATGCAT 3 cut(s) 751, 1168, 2386
EcoT38I GRGCYC 1 cut(s) 2492
ErhI CCWWGG 3 cut(s) 211, 1833, 2236
FalI AAGNNNNNCTT 2 cut(s) 828, 860
FaqI GGGAC 2 cut(s) 237, 509
FauI CCCGC 2 cut(s) 2200, 2275
FauNDI CATATG 2 cut(s) 1126, 2541
FbaI TGATCA 2 cut(s) 6, 1648
FblI GTMKAC 1 cut(s) 2221
FokI GGATG 9 cut(s) 193, 404, 626, 847, 1850, 1856, 2171, 2342, 2360
FriOI GRGCYC 1 cut(s) 2492
FspBI CTAG 2 cut(s) 936, 2070
FspI TGCGCA 1 cut(s) 164
GlaI GCGC 2 cut(s) 164, 1593
GsaI CCCAGC 2 cut(s) 1223, 1930
GsuI CTGGAG 2 cut(s) 962, 1023
HaeIII GGCC 7 cut(s) 235, 653, 843, 1575, 1894, 2490, 2631
HapII CCGG 1 cut(s) 2527
HgaI GACGC 1 cut(s) 83
HhaI GCGC 2 cut(s) 165, 1594
Hin1I GRCGYC 2 cut(s) 94, 2177
Hin6I GCGC 2 cut(s) 163, 1592
HinP1I GCGC 2 cut(s) 163, 1592
HincII GTYRAC 2 cut(s) 1830, 2222
HindII GTYRAC 2 cut(s) 1830, 2222
HindIII AAGCTT 1 cut(s) 885
HpaII CCGG 1 cut(s) 2527
HphI GGTGA 5 cut(s) 470, 634, 1232, 2324, 2555
Hpy166II GTNNAC 5 cut(s) 1830, 2222, 2281, 2464, 2483
Hpy8I GTNNAC 5 cut(s) 1830, 2222, 2281, 2464, 2483
Hpy99I CGWCG 1 cut(s) 99
HpyAV CCTTC 5 cut(s) 214, 1109, 1448, 1573, 1716
HpyCH4III ACNGT 7 cut(s) 483, 628, 732, 1090, 1326, 2461, 2616
HpyCH4IV ACGT 3 cut(s) 58, 931, 2177
HpyF10VI GCNNNNNNNGC 9 cut(s) 185, 596, 815, 1172, 1622, 2192, 2581, 2584, 2587
HpyF3I CTNAG 7 cut(s) 78, 357, 1019, 1201, 1464, 1884, 2598
HpySE526I ACGT 3 cut(s) 58, 931, 2177
Hsp92I GRCGYC 2 cut(s) 94, 2177
HspAI GCGC 2 cut(s) 163, 1592
KroI GCCGGC 1 cut(s) 2526
KroNI GCCGGC 1 cut(s) 2528
Ksp22I TGATCA 2 cut(s) 6, 1648
Kzo9I GATC 7 cut(s) 6, 238, 318, 857, 1605, 1648, 2551
LmnI GCTCC 4 cut(s) 37, 46, 1004, 1015
LweI GCATC 6 cut(s) 557, 758, 1024, 1199, 1300, 2147
MabI ACCWGGT 1 cut(s) 2248
MaeI CTAG 2 cut(s) 936, 2070
MaeII ACGT 3 cut(s) 58, 931, 2177
MaeIII GTNAC 7 cut(s) 135, 458, 530, 622, 719, 1238, 1245
MalI GATC 7 cut(s) 8, 240, 320, 859, 1607, 1650, 2553
MboI GATC 7 cut(s) 6, 238, 318, 857, 1605, 1648, 2551
MflI RGATCY 1 cut(s) 1605
MhlI GDGCHC 2 cut(s) 55, 2492
MlsI TGGCCA 1 cut(s) 1575
MluCI AATT 9 cut(s) 14, 158, 375, 894, 1134, 1685, 1753, 2296, 2534
MluNI TGGCCA 1 cut(s) 1575
MlyI GAGTC 4 cut(s) 491, 1030, 2294, 2312
MmeI TCCRAC 5 cut(s) 63, 77, 1253, 2071, 2325
Mox20I TGGCCA 1 cut(s) 1575
Mph1103I ATGCAT 3 cut(s) 751, 1168, 2386
MroNI GCCGGC 1 cut(s) 2526
MscI TGGCCA 1 cut(s) 1575
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 1575
MspA1I CMGCKG 5 cut(s) 441, 1301, 2195, 2340, 2578
MspCI CTTAAG 1 cut(s) 2449
MspI CCGG 1 cut(s) 2527
MspR9I CCNGG 6 cut(s) 650, 941, 1251, 1610, 2250, 2478
Mva1269I GAATGC 5 cut(s) 918, 1254, 1357, 1555, 2384
MvaI CCWGG 6 cut(s) 650, 941, 1251, 1610, 2250, 2478
MvnI CGCG 2 cut(s) 1594, 2430
MwoI GCNNNNNNNGC 9 cut(s) 185, 596, 815, 1172, 1622, 2192, 2581, 2584, 2587
NaeI GCCGGC 1 cut(s) 2528
NcoI CCATGG 1 cut(s) 1833
NdeI CATATG 2 cut(s) 1126, 2541
NdeII GATC 7 cut(s) 6, 238, 318, 857, 1605, 1648, 2551
NgoMIV GCCGGC 1 cut(s) 2526
NlaIV GGNNCC 8 cut(s) 225, 572, 1607, 1938, 2040, 2100, 2361, 2490
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 3 cut(s) 458, 622, 1238
NsbI TGCGCA 1 cut(s) 164
NsiI ATGCAT 3 cut(s) 751, 1168, 2386
NspI RCATGY 2 cut(s) 1199, 1284
OliI CACNNNNGTG 1 cut(s) 174
PaeR7I CTCGAG 1 cut(s) 1546
PagI TCATGA 1 cut(s) 301
PceI AGGCCT 2 cut(s) 843, 1894
PcsI WCGNNNNNNNCGW 3 cut(s) 238, 640, 904
PctI GAATGC 5 cut(s) 918, 1254, 1357, 1555, 2384
PdiI GCCGGC 1 cut(s) 2528
PfeI GAWTC 7 cut(s) 362, 709, 1225, 1468, 1506, 1670, 2317
PflMI CCANNNNNTGG 2 cut(s) 566, 1933
PleI GAGTC 4 cut(s) 491, 1029, 2293, 2311
PpsI GAGTC 4 cut(s) 491, 1029, 2293, 2311
Ppu21I YACGTR 1 cut(s) 932
PpuMI RGGWCCY 1 cut(s) 224
PsiI TTATAA 1 cut(s) 2295
Psp1406I AACGTT 1 cut(s) 58
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 6 cut(s) 648, 939, 1249, 1608, 2248, 2476
PspEI GGTNACC 1 cut(s) 458
PspFI CCCAGC 2 cut(s) 1219, 1926
PspGI CCWGG 6 cut(s) 648, 939, 1249, 1608, 2248, 2476
PspN4I GGNNCC 8 cut(s) 225, 572, 1607, 1938, 2040, 2100, 2361, 2490
PspOMI GGGCCC 1 cut(s) 2488
PspPI GGNCC 7 cut(s) 224, 1808, 2099, 2147, 2240, 2488, 2489
PspPPI RGGWCCY 1 cut(s) 224
PstI CTGCAG 1 cut(s) 2583
PstNI CAGNNNCTG 1 cut(s) 2108
PsuI RGATCY 1 cut(s) 1605
PvuII CAGCTG 3 cut(s) 1301, 2340, 2578
RsaI GTAC 4 cut(s) 934, 1140, 2010, 2068
RsaNI GTAC 4 cut(s) 933, 1139, 2009, 2067
RseI CAYNNNNRTG 1 cut(s) 174
SalI GTCGAC 1 cut(s) 2220
Sau3AI GATC 7 cut(s) 6, 238, 318, 857, 1605, 1648, 2551
Sau96I GGNCC 7 cut(s) 224, 1808, 2099, 2147, 2240, 2488, 2489
ScaI AGTACT 2 cut(s) 2010, 2068
SchI GAGTC 4 cut(s) 491, 1030, 2294, 2312
ScrFI CCNGG 6 cut(s) 650, 941, 1251, 1610, 2250, 2478
SduI GDGCHC 2 cut(s) 55, 2492
SexAI ACCWGGT 1 cut(s) 2248
SfaNI GCATC 6 cut(s) 557, 758, 1024, 1199, 1300, 2147
SfcI CTRYAG 2 cut(s) 192, 2579
Sfr274I CTCGAG 1 cut(s) 1546
SinI GGWCC 5 cut(s) 224, 1808, 2099, 2147, 2240
SlaI CTCGAG 1 cut(s) 1546
SmiI ATTTAAAT 1 cut(s) 1945
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 3 cut(s) 526, 1546, 2449
SmoI CTYRAG 3 cut(s) 526, 1546, 2449
Sse9I AATT 9 cut(s) 14, 158, 375, 894, 1134, 1685, 1753, 2296, 2534
SseBI AGGCCT 2 cut(s) 843, 1894
SsiI CCGC 8 cut(s) 148, 439, 1613, 2156, 2193, 2243, 2268, 2632
SspI AATATT 1 cut(s) 1536
SspMI CTAG 2 cut(s) 936, 2070
StuI AGGCCT 2 cut(s) 843, 1894
StyD4I CCNGG 6 cut(s) 648, 939, 1249, 1608, 2248, 2476
StyI CCWWGG 3 cut(s) 211, 1833, 2236
SwaI ATTTAAAT 1 cut(s) 1945
TaaI ACNGT 7 cut(s) 483, 628, 732, 1090, 1326, 2461, 2616
TaiI ACGT 3 cut(s) 61, 934, 2180
TaqI TCGA 7 cut(s) 66, 373, 634, 1063, 1547, 2221, 2547
TasI AATT 9 cut(s) 14, 158, 375, 894, 1134, 1685, 1753, 2296, 2534
TatI WGTACW 3 cut(s) 1138, 2008, 2066
TauI GCSGC 2 cut(s) 1616, 2634
TfiI GAWTC 7 cut(s) 362, 709, 1225, 1468, 1506, 1670, 2317
TscAI CASTG 5 cut(s) 181, 982, 1312, 2328, 2466
TseFI GTSAC 3 cut(s) 458, 622, 1238
Tsp45I GTSAC 3 cut(s) 458, 622, 1238
TspDTI ATGAA 7 cut(s) 428, 1082, 1464, 1498, 1727, 1869, 2583
TspGWI ACGGA 1 cut(s) 896
TspRI CASTG 5 cut(s) 181, 982, 1312, 2328, 2466
Van91I CCANNNNNTGG 2 cut(s) 566, 1933
Vha464I CTTAAG 1 cut(s) 2449
VpaK11BI GGWCC 5 cut(s) 224, 1808, 2099, 2147, 2240
XagI CCTNNNNNAGG 1 cut(s) 848
XapI RAATTY 2 cut(s) 1685, 2534
XceI RCATGY 2 cut(s) 1199, 1284
XcmI CCANNNNNNNNNTGG 1 cut(s) 1765
XhoI CTCGAG 1 cut(s) 1546
XmiI GTMKAC 1 cut(s) 2221
XspI CTAG 2 cut(s) 936, 2070
ZraI GACGTC 1 cut(s) 2178
ZrmI AGTACT 2 cut(s) 2010, 2068
Zsp2I ATGCAT 3 cut(s) 751, 1168, 2386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.