pycom03g12030

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0008095
Physical Location & Seq
Forward (+)
12364164 .. 12367487
3324 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g12030.1

Sequence Viewer

Length: 2748 bp
ATGGCTTCTACAGGTTCAAACACATCTCAGGGTGTGAAGGGGCCAATTAGGCCTGATCGGGTTACAAAAATAAGACTGCAATTTAAAACATTCCTTTCAGAAGCAACTGGATTTTATCATGATTTAATAGTGAAGATCAGAGCAAAGTATGGGCTTCCTCTAGGTTATTTCTCTGAAGATTCTGAAAACCGGACTGTTATAGACAAGGATGGAAAGAAAGGTCTAATTTCATGCCACAGATGCTTGATATATTTGGGTGACCTTGCACGCTACAAAGGACTGTACGGGGAAGGGGACTCAAAAACTCGTGAGTATGCTGCAGCCTCAAGTTACTATATGCAAGCTGCATCTCTCTGGCCATCCAGTGGGAACCCCCATCATCAGCTTGCTATATTGGCTTCCTATTCGGGGGATGAGTTGGTGGCTGTTTATCGATATTTTCGTAGCCTGGCGGTGGATAGTCCCTTTTCAACTGCAAGAGATAACTTGATTGTTGCATTTGAGAAGAATCGTCAGAGTTACTCTCAACTGTCTGGGAATACTAATGCTTCCGCAGTCAAAGAGTTGCCAGCACGGTTGGCAGGCAAAGGTAGAGGGAAAGGGGAAACAATACCCACATCTAAAGGTAACAATACCGAAGTGAGCCTGCTTAACGAAAGAGCATCCAGTACCCAGGAGACTTATAAGGCTTTTTGCATCCGTTTTGTCCGTCTAAATGGCATTCTTTTCACTCGTACTAGCCTGGAGACATTTGCAGAAGTTCTAACTGTGGTAAGCAGTGGCTTATGTGAGCTTCTGTCTTCTGGAGGAGAAGAGGTGCTGAATTTTGGTGCAGATGCTGTTGAAAATGGACTTGTTATTGTTAGACTTATCTCCATATTGATATTCACAGTTCATAATGTGAAAAAGGAAAGCGAAGGTCAGACATATGCAGAAATTGTACAACGTGCTGTCCTGCTGCAGAATGCATTTACTGCTGTTTTTGAGTTGATGGGACACATCTTAGAGAGATGTGTGCAGTTGTGTGATCCTTCTTCAAGTTTCCTGTTACCAGGTATTTTGGTTTTTGTAGAATGGTTGGCATGCTGTCCTGATGTTGCAGCTGGAAGTGATGCAGACGAAAAACAGTCGTCTGTTAGATCCAAATTCTGGAATGTTTGCATATCCTTCTTCAATAGTCTCTCGTCTACTGGGCCAATGTTGATAGATGATGATGAAGATGAGACTTGCTTTAATAACATGAGCAGATACGAAGAAGGTGAAACTGAAAACCGTCTTGCTCTGTGGGAGGATTTTGAGTTGAGAGGATTTATTCCACTAATTCCAGCACAAACAATATTGGACTTCTCAAGGAAGCATTCTTTTGGAAGTGATGGCCATAAGGAAAAAGGGGCCCGTGTTAAGAGGATTCTGGCAGCAGGCAAAGCTCTTGCAAATGTGGTAAAGGTTGACCAGGAAGCAGTATATTTTGATTCGAAGTTAAAGAAATTTGTCGTCGGTTTTGAGCCTCAAGTGCAAAATGATACTGTTCCTACCTCCTATCTACATATGGCTAATGGAAATGATAATCTGCAAGAAAATCAAGTAGAGGGCACAATCAGTTTGGGAGCTGCATATCCAAAGCCAGAGTTATCCATGGAAGGGGATGAGGAAGATGAAGTAATAGTCTTTAAGCCAATAGTGGCTGAGAAGCGACCTGATGCAGTAAGCACCACATGGACAGCCTATGACGGCCTAGAGCCTGGTAAAAATGCTTCTCCAGGTGATCAAACAGTTAATGTAACTTATGGTTCTGCCGCTTTGGATAATCTACACCATCAAAATGCCTTTAGGGCTGATTTACAAATACCTGTATCTTTTGCAAATGGTATACCCCAACACTTGCAACCAATCCAGTTACATGGTTCCAAGTTTTCAGTGGATACTGGTTTTGGTGTTGGTTCACAACTACCTGCATCAATTACCAATACTGTCCCCCTGAATTTGCAACCAGTTCAATCAAATTCTTTGAATCTCGCAATGGAGGAAGAAATGTCTCTTGCTAATAGCTTAAAGAGTATGGGATTTATGGGGAATGGGCATGCACTAAAATCTGAGCCTGTAGCAGTTTCTGGTCCATACCAGCACCCAGTGAATGGTAGTACTGGTGGTATGTTTCACAGCCACACAAAAGCTCCAGAAAATCTTTTGCCTTATAAAGTTGATGCAACTTATGGAGCAATTGCTGACGGGTTGACTGTAAAGACATCATCAGCTCTGCCCACAGGTATCCGAAAAAGTCCAGTAAGTCGACCTGTTAGGCACCTTGGTCCACCACCTGGTTTTACCCGCATTCCTCCCAAAAATGCGAATGAATCAATTTATAGTTCAGATTCAATGAGTGAGAGTCTAGTGATGGATGATTACAGCTGGTTGGATGGATATCAGATGCCATCTTCAACAAAAGCAAATGGGCTCAATGATTATGTTAGTTATTCATCTCAATACAATCCCCATCGCTTCACAAACAGCAACAGCTTAAGTGGAACAGTGAACTTCCCCTTTCCTGGTAAACAGGGTCCACCCATGCAACTACCAGGGGAAACCCAGAAAAGCCGGCAAGATATCCAGAGGCTTGATGACCTCAAGCTACACCATGAAATGCAGCTGCAGCAACAGCAACAACTTGTAAATGGAAATCAGAACTTGAATCCACAGCCTGAGCAATATCAAGGACAGTCTCTCTGGATGGGCCGCCATTTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

916

Amino Acids

100.42

Weight (kDa)

5.82

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 22 - 95 1.6e-12 Telomerase activating protein Est1
EST1_DNA_bind PF10373 108 - 443 2.2e-65 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 684, 2196
Acc36I ACCTGC 1 cut(s) 1960
AccB1I GGYRCC 1 cut(s) 2301
AccB7I CCANNNNNTGG 4 cut(s) 365, 1149, 2135, 2318
AccI GTMKAC 3 cut(s) 1187, 1870, 2290
AciI CCGC 5 cut(s) 452, 552, 1797, 2329, 2734
AclWI GGATC 2 cut(s) 1022, 1134
AcoI YGGCCR 2 cut(s) 356, 1375
AcsI RAATTY 5 cut(s) 823, 1145, 1487, 1981, 2002
AcuI CTGAAG 1 cut(s) 195
AdeI CACNNNGTG 1 cut(s) 2131
AfaI GTAC 5 cut(s) 284, 670, 736, 942, 2143
AfiI CCNNNNNNNGG 8 cut(s) 365, 408, 454, 1149, 1641, 2135, 2318, 2546
AflII CTTAAG 1 cut(s) 2518
Alw26I GTCTC 6 cut(s) 671, 740, 1184, 1217, 2040, 2724
AlwI GGATC 2 cut(s) 1022, 1134
AlwNI CAGNNNCTG 2 cut(s) 839, 2111
AoxI GGCC 8 cut(s) 41, 50, 356, 1193, 1375, 1392, 1732, 2731
ApaI GGGCCC 1 cut(s) 1396
ApoI RAATTY 5 cut(s) 823, 1145, 1487, 1981, 2002
AspS9I GGNCC 8 cut(s) 41, 1193, 1392, 1393, 2114, 2309, 2558, 2731
AsuHPI GGTGA 3 cut(s) 269, 1271, 1775
AsuII TTCGAA 1 cut(s) 1475
AvaII GGWCC 3 cut(s) 2114, 2309, 2558
BaeGI GKGCMC 2 cut(s) 1396, 1595
BalI TGGCCA 2 cut(s) 358, 1377
BanI GGYRCC 1 cut(s) 2301
BanII GRGCYC 2 cut(s) 1396, 2457
BarI GAAGNNNNNNTAC 2 cut(s) 1738, 1770
BauI CACGAG 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 792
BceAI ACGGC 1 cut(s) 1747
BciVI GTATCC 2 cut(s) 1915, 2279
BclI TGATCA 1 cut(s) 1765
BcoDI GTCTC 6 cut(s) 671, 740, 1184, 1217, 2040, 2724
BfaI CTAG 4 cut(s) 161, 738, 1736, 2390
BfmI CTRYAG 5 cut(s) 9, 318, 959, 2100, 2648
BfrI CTTAAG 1 cut(s) 2518
BfuAI ACCTGC 1 cut(s) 1960
BfuI GTATCC 2 cut(s) 1915, 2279
BglI GCCNNNNNGGC 2 cut(s) 49, 1832
BmcAI AGTACT 1 cut(s) 2143
Bme18I GGWCC 3 cut(s) 2114, 2309, 2558
BmgT120I GGNCC 8 cut(s) 41, 1193, 1392, 1393, 2114, 2309, 2558, 2731
BmiI GGNNCC 7 cut(s) 42, 371, 1393, 1394, 1906, 2303, 2559
BmrI ACTGGG 2 cut(s) 1200, 2123
BmuI ACTGGG 2 cut(s) 1200, 2123
BpiI GAAGAC 1 cut(s) 792
BplI GAGNNNNNCTC 2 cut(s) 508, 540
BpmI CTGGAG 4 cut(s) 764, 825, 1743, 2160
Bpu10I CCTNAGC 1 cut(s) 2700
Bpu14I TTCGAA 1 cut(s) 1475
BpuEI CTTGAG 4 cut(s) 310, 1333, 1494, 2609
Bsa29I ATCGAT 1 cut(s) 433
BsaBI GATNNNNATC 1 cut(s) 2421
BsaJI CCNNGG 4 cut(s) 672, 1635, 2305, 2576
BsaWI WCCGGW 1 cut(s) 189
BsaXI ACNNNNNCTCC 4 cut(s) 801, 831, 2158, 2188
Bsc4I CCNNNNNNNGG 8 cut(s) 365, 408, 454, 1149, 1641, 2135, 2318, 2546
Bse118I RCCGGY 1 cut(s) 2595
Bse3DI GCAATG 1 cut(s) 2025
Bse8I GATNNNNATC 1 cut(s) 2421
BseCI ATCGAT 1 cut(s) 433
BseDI CCNNGG 4 cut(s) 672, 1635, 2305, 2576
BseGI GGATG 9 cut(s) 214, 359, 418, 662, 696, 1651, 2404, 2422, 2733
BseJI GATNNNNATC 1 cut(s) 2421
BseLI CCNNNNNNNGG 8 cut(s) 365, 408, 454, 1149, 1641, 2135, 2318, 2546
BseMI GCAATG 1 cut(s) 2025
BseMII CTCAG 4 cut(s) 41, 1677, 2085, 2691
BseRI GAGGAG 1 cut(s) 822
BseSI GKGCMC 2 cut(s) 1396, 1595
BsgI GTGCAG 2 cut(s) 852, 1037
BshFI GGCC 8 cut(s) 43, 52, 358, 1195, 1377, 1394, 1734, 2733
BshNI GGYRCC 1 cut(s) 2301
BshVI ATCGAT 1 cut(s) 433
BsiSI CCGG 2 cut(s) 190, 2596
BslFI GGGAC 4 cut(s) 308, 447, 1008, 1958
BslI CCNNNNNNNGG 8 cut(s) 365, 408, 454, 1149, 1641, 2135, 2318, 2546
BsmAI GTCTC 6 cut(s) 671, 740, 1184, 1217, 2040, 2724
BsmFI GGGAC 4 cut(s) 308, 447, 1008, 1958
BsmI GAATGC 4 cut(s) 720, 970, 1357, 2331
BsnI GGCC 8 cut(s) 43, 52, 358, 1195, 1377, 1394, 1734, 2733
Bsp119I TTCGAA 1 cut(s) 1475
Bsp120I GGGCCC 1 cut(s) 1392
Bsp1286I GDGCHC 3 cut(s) 1396, 1595, 2457
Bsp1407I TGTACA 1 cut(s) 940
Bsp143I GATC 5 cut(s) 55, 135, 1027, 1139, 1765
Bsp19I CCATGG 1 cut(s) 1635
BspACI CCGC 5 cut(s) 452, 552, 1797, 2329, 2734
BspANI GGCC 8 cut(s) 43, 52, 358, 1195, 1377, 1394, 1734, 2733
BspCNI CTCAG 4 cut(s) 40, 1678, 2086, 2692
BspDI ATCGAT 1 cut(s) 433
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 7 cut(s) 42, 371, 1393, 1394, 1906, 2303, 2559
BspMAI CTGCAG 3 cut(s) 322, 963, 2652
BspMI ACCTGC 1 cut(s) 1960
BspPI GGATC 2 cut(s) 1022, 1134
BspT104I TTCGAA 1 cut(s) 1475
BspT107I GGYRCC 1 cut(s) 2301
BspTI CTTAAG 1 cut(s) 2518
BsrDI GCAATG 1 cut(s) 2025
BsrFI RCCGGY 1 cut(s) 2595
BsrGI TGTACA 1 cut(s) 940
BssAI RCCGGY 1 cut(s) 2595
BssECI CCNNGG 4 cut(s) 672, 1635, 2305, 2576
BssMI GATC 5 cut(s) 55, 135, 1027, 1139, 1765
BssNAI GTATAC 1 cut(s) 1871
BssSI CACGAG 1 cut(s) 306
BssT1I CCWWGG 2 cut(s) 1635, 2305
Bst1107I GTATAC 1 cut(s) 1871
Bst2BI CACGAG 1 cut(s) 306
Bst6I CTCTTC 1 cut(s) 807
BstAFI CTTAAG 1 cut(s) 2518
BstAPI GCANNNNNTGC 1 cut(s) 974
BstAUI TGTACA 1 cut(s) 940
BstBI TTCGAA 1 cut(s) 1475
BstDEI CTNAG 5 cut(s) 27, 1003, 1686, 2094, 2700
BstDSI CCRYGG 1 cut(s) 1635
BstEII GGTNACC 1 cut(s) 257
BstF5I GGATG 9 cut(s) 214, 359, 418, 662, 696, 1651, 2404, 2422, 2733
BstKTI GATC 5 cut(s) 58, 138, 1030, 1142, 1768
BstMAI GTCTC 6 cut(s) 671, 740, 1184, 1217, 2040, 2724
BstMBI GATC 5 cut(s) 55, 135, 1027, 1139, 1765
BstNSI RCATGY 2 cut(s) 1086, 2084
BstPI GGTNACC 1 cut(s) 257
BstSFI CTRYAG 5 cut(s) 9, 318, 959, 2100, 2648
BstSLI GKGCMC 2 cut(s) 1396, 1595
BstV2I GAAGAC 1 cut(s) 792
BstX2I RGATCY 1 cut(s) 1139
BstXI CCANNNNNNTGG 1 cut(s) 1901
BstYI RGATCY 1 cut(s) 1139
BstZ17I GTATAC 1 cut(s) 1871
Bsu15I ATCGAT 1 cut(s) 433
BsuI GTATCC 2 cut(s) 1915, 2279
BsuRI GGCC 8 cut(s) 43, 52, 358, 1195, 1377, 1394, 1734, 2733
BsuTUI ATCGAT 1 cut(s) 433
BtgI CCRYGG 1 cut(s) 1635
BtgZI GCGATG 1 cut(s) 2480
BtsCI GGATG 9 cut(s) 214, 359, 418, 662, 696, 1651, 2404, 2422, 2733
BtsI GCAGTG 1 cut(s) 784
BtsIMutI CAGTG 5 cut(s) 370, 784, 1923, 2136, 2535
BveI ACCTGC 1 cut(s) 1960
CaiI CAGNNNCTG 2 cut(s) 839, 2111
CciI TCATGA 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 2595
Cfr13I GGNCC 8 cut(s) 41, 1193, 1392, 1393, 2114, 2309, 2558, 2731
ClaI ATCGAT 1 cut(s) 433
CsiI ACCWGGT 2 cut(s) 1051, 2317
Csp6I GTAC 5 cut(s) 283, 669, 735, 941, 2142
CviQI GTAC 5 cut(s) 283, 669, 735, 941, 2142
DdeI CTNAG 5 cut(s) 27, 1003, 1686, 2094, 2700
DpnI GATC 5 cut(s) 57, 137, 1029, 1141, 1767
DpnII GATC 5 cut(s) 55, 135, 1027, 1139, 1765
DraI TTTAAA 1 cut(s) 85
DraIII CACNNNGTG 1 cut(s) 2131
EaeI YGGCCR 2 cut(s) 356, 1375
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
Eco130I CCWWGG 2 cut(s) 1635, 2305
Eco147I AGGCCT 1 cut(s) 52
Eco24I GRGCYC 2 cut(s) 1396, 2457
Eco32I GATATC 2 cut(s) 2423, 2605
Eco47I GGWCC 3 cut(s) 2114, 2309, 2558
Eco57I CTGAAG 1 cut(s) 195
Eco91I GGTNACC 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 1392
EcoO65I GGTNACC 1 cut(s) 257
EcoRV GATATC 2 cut(s) 2423, 2605
EcoT14I CCWWGG 2 cut(s) 1635, 2305
EcoT22I ATGCAT 1 cut(s) 970
EcoT38I GRGCYC 2 cut(s) 1396, 2457
ErhI CCWWGG 2 cut(s) 1635, 2305
FaqI GGGAC 4 cut(s) 308, 447, 1008, 1958
FauI CCCGC 1 cut(s) 2336
FauNDI CATATG 2 cut(s) 928, 1548
FbaI TGATCA 1 cut(s) 1765
FblI GTMKAC 3 cut(s) 1187, 1870, 2290
FokI GGATG 9 cut(s) 221, 346, 425, 649, 683, 1658, 2411, 2429, 2740
FriOI GRGCYC 2 cut(s) 1396, 2457
FspBI CTAG 4 cut(s) 161, 738, 1736, 2390
GsuI CTGGAG 4 cut(s) 764, 825, 1743, 2160
HaeIII GGCC 8 cut(s) 43, 52, 358, 1195, 1377, 1394, 1734, 2733
HapII CCGG 2 cut(s) 190, 2596
HincII GTYRAC 3 cut(s) 1450, 2235, 2291
HindII GTYRAC 3 cut(s) 1450, 2235, 2291
HpaII CCGG 2 cut(s) 190, 2596
HphI GGTGA 3 cut(s) 269, 1271, 1775
Hpy188III TCNNGA 8 cut(s) 119, 308, 804, 1091, 1150, 2177, 2608, 2725
Hpy99I CGWCG 1 cut(s) 1499
HpyAV CCTTC 7 cut(s) 31, 284, 911, 1041, 1177, 1250, 1634
HpyCH4IV ACGT 1 cut(s) 946
HpyF3I CTNAG 5 cut(s) 27, 1003, 1686, 2094, 2700
HpySE526I ACGT 1 cut(s) 946
KroI GCCGGC 1 cut(s) 2595
KroNI GCCGGC 1 cut(s) 2597
Ksp22I TGATCA 1 cut(s) 1765
Kzo9I GATC 5 cut(s) 55, 135, 1027, 1139, 1765
LmnI GCTCC 3 cut(s) 1607, 2179, 2216
MabI ACCWGGT 2 cut(s) 1051, 2317
MaeI CTAG 4 cut(s) 161, 738, 1736, 2390
MaeII ACGT 1 cut(s) 946
MaeIII GTNAC 8 cut(s) 61, 257, 329, 518, 626, 1047, 1780, 1896
MalI GATC 5 cut(s) 57, 137, 1029, 1141, 1767
MboI GATC 5 cut(s) 55, 135, 1027, 1139, 1765
MfeI CAATTG 1 cut(s) 2220
MflI RGATCY 1 cut(s) 1139
MhlI GDGCHC 3 cut(s) 1396, 1595, 2457
MlsI TGGCCA 2 cut(s) 358, 1377
MluNI TGGCCA 2 cut(s) 358, 1377
MlyI GAGTC 2 cut(s) 290, 2395
MmeI TCCRAC 1 cut(s) 2394
Mox20I TGGCCA 2 cut(s) 358, 1377
Mph1103I ATGCAT 1 cut(s) 970
MroNI GCCGGC 1 cut(s) 2595
MscI TGGCCA 2 cut(s) 358, 1377
MslI CAYNNNNRTG 1 cut(s) 1821
Msp20I TGGCCA 2 cut(s) 358, 1377
MspA1I CMGCKG 3 cut(s) 1103, 2409, 2647
MspCI CTTAAG 1 cut(s) 2518
MspI CCGG 2 cut(s) 190, 2596
MunI CAATTG 1 cut(s) 2220
Mva1269I GAATGC 4 cut(s) 720, 970, 1357, 2331
NaeI GCCGGC 1 cut(s) 2597
NcoI CCATGG 1 cut(s) 1635
NdeI CATATG 2 cut(s) 928, 1548
NdeII GATC 5 cut(s) 55, 135, 1027, 1139, 1765
NgoMIV GCCGGC 1 cut(s) 2595
NlaIV GGNNCC 7 cut(s) 42, 371, 1393, 1394, 1906, 2303, 2559
NmuCI GTSAC 1 cut(s) 257
NsiI ATGCAT 1 cut(s) 970
NspI RCATGY 2 cut(s) 1086, 2084
NspV TTCGAA 1 cut(s) 1475
PaeI GCATGC 2 cut(s) 1086, 2084
PagI TCATGA 1 cut(s) 118
PceI AGGCCT 1 cut(s) 52
PcsI WCGNNNNNNNCGW 1 cut(s) 439
PctI GAATGC 4 cut(s) 720, 970, 1357, 2331
PdiI GCCGGC 1 cut(s) 2597
PfeI GAWTC 8 cut(s) 179, 508, 1408, 1472, 2011, 2354, 2372, 2689
PflMI CCANNNNNTGG 4 cut(s) 365, 1149, 2135, 2318
PleI GAGTC 2 cut(s) 290, 2394
PpsI GAGTC 2 cut(s) 290, 2394
PsiI TTATAA 2 cut(s) 684, 2196
PspEI GGTNACC 1 cut(s) 257
PspN4I GGNNCC 7 cut(s) 42, 371, 1393, 1394, 1906, 2303, 2559
PspOMI GGGCCC 1 cut(s) 1392
PspPI GGNCC 8 cut(s) 41, 1193, 1392, 1393, 2114, 2309, 2558, 2731
PstI CTGCAG 3 cut(s) 322, 963, 2652
PstNI CAGNNNCTG 2 cut(s) 839, 2111
PsuI RGATCY 1 cut(s) 1139
PvuII CAGCTG 3 cut(s) 1103, 2409, 2647
RsaI GTAC 5 cut(s) 284, 670, 736, 942, 2143
RsaNI GTAC 5 cut(s) 283, 669, 735, 941, 2142
RseI CAYNNNNRTG 1 cut(s) 1821
SalI GTCGAC 1 cut(s) 2289
Sau3AI GATC 5 cut(s) 55, 135, 1027, 1139, 1765
Sau96I GGNCC 8 cut(s) 41, 1193, 1392, 1393, 2114, 2309, 2558, 2731
ScaI AGTACT 1 cut(s) 2143
SchI GAGTC 2 cut(s) 290, 2395
SduI GDGCHC 3 cut(s) 1396, 1595, 2457
SexAI ACCWGGT 2 cut(s) 1051, 2317
SfcI CTRYAG 5 cut(s) 9, 318, 959, 2100, 2648
SfiI GGCCNNNNNGGCC 1 cut(s) 49
SfuI TTCGAA 1 cut(s) 1475
SinI GGWCC 3 cut(s) 2114, 2309, 2558
SmiMI CAYNNNNRTG 1 cut(s) 1821
SmlI CTYRAG 5 cut(s) 325, 1348, 1509, 2518, 2624
SmoI CTYRAG 5 cut(s) 325, 1348, 1509, 2518, 2624
SphI GCATGC 2 cut(s) 1086, 2084
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 5 cut(s) 452, 552, 1797, 2329, 2734
SspI AATATT 1 cut(s) 1338
SspMI CTAG 4 cut(s) 161, 738, 1736, 2390
StuI AGGCCT 1 cut(s) 52
StyI CCWWGG 2 cut(s) 1635, 2305
TaiI ACGT 1 cut(s) 949
TaqI TCGA 3 cut(s) 433, 1475, 2290
TatI WGTACW 2 cut(s) 940, 2141
TauI GCSGC 2 cut(s) 1799, 2736
TfiI GAWTC 8 cut(s) 179, 508, 1408, 1472, 2011, 2354, 2372, 2689
TscAI CASTG 5 cut(s) 370, 784, 1923, 2136, 2535
TseFI GTSAC 1 cut(s) 257
Tsp45I GTSAC 1 cut(s) 257
TspDTI ATGAA 7 cut(s) 219, 884, 1230, 1671, 2367, 2466, 2652
TspGWI ACGGA 2 cut(s) 689, 698
TspRI CASTG 5 cut(s) 370, 784, 1923, 2136, 2535
Van91I CCANNNNNTGG 4 cut(s) 365, 1149, 2135, 2318
Vha464I CTTAAG 1 cut(s) 2518
VpaK11BI GGWCC 3 cut(s) 2114, 2309, 2558
XapI RAATTY 5 cut(s) 823, 1145, 1487, 1981, 2002
XceI RCATGY 2 cut(s) 1086, 2084
XcmI CCANNNNNNNNNTGG 1 cut(s) 1915
XmiI GTMKAC 3 cut(s) 1187, 1870, 2290
XspI CTAG 4 cut(s) 161, 738, 1736, 2390
ZrmI AGTACT 1 cut(s) 2143
Zsp2I ATGCAT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.