FvH4_3g32600

Root cap

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002778
Physical Location & Seq
Forward (+)
27904173 .. 27905972
1800 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g32600.t2

Sequence Viewer

Length: 1374 bp
ATGGCTAAACTAAGAGGGTCTCACATATTTGTGGCTCTCTGTGTGGTGCTCATTGCCATGGAAACCAGCTTTGTTCTGGGCCAAGGCAATGGAAATGGGAATGGGAATGGAAACACCGGCAGTGGCAACGGAAATGGGAATGGCAATGGAAATACTGGTGGTGGAAACGGGAATGGCAACGGCAACGGAAACACTGGCAGTGGCAATGGAAACGGAAACGGGAACGGCAATACTGGCAGTGGTGACGGTAATGGCAATGGGAACGGAAACGGAAATGGCAACGGGAATGGCAATGGCAACGGCAATGGCAATGGTAACGGAAATGGAAATGGTAATGGAAACGGCAATGGTAATGGAAATGGAAACGGCAACGGTAATGGCAACGGCAACGGGAATGGAAATGGCAACGGCAACGGAAATACAATCAGTGATACAACAAATTACGACGTACTGCCGTCATTAGCCACAGGACAGGAGCAATGCATGTGCAAAGCACAGGGTGCCTGCTTTTACAAGACCCTTGTTTGTCCACCGGAATGTCCCGAGAGAAAGCCTAAAAGCAACAAGAGAACAAAGGCTTGCTTCGTCAACTGCGGTAGCAAGTGTGAAGTTACTTGCAAGGTTAGAAGAGGTAACTGCGATGGGTATGGCTCTATCTGCTACGACCCTCGATTTATTGGCGGTGACGGTGTGATGTTCTACTTCCATGGAACCAAGGGAGGAAACTTTGCCGTTGTCTCAGACGAAAACCTTCATATCAATGCACACTTCATTGGAACCCGTCCTGAAGGGAGGACTCGTGACTTCACATGGATTCAAGCCATCTCGGTCATGTTTGATATTCACACTCTTGTCATTGCAGCAAAGAGAGTCTCAAAGTGGGATGACAAAGTTGATGCTCTGGTGGTAAAGTGGGATGGTGAGTCAGTAGACATCCCCGAATACGGCGAGGCTGAATGGAGGATGAATGGTGAAGACAGAGAGGTCATTGTCGAAAGAACTGATGATGCGAATTACGCGAGGGTTATTGTGTCTGATCTGGTTGAAATAACCGTTAGGGTTAGGCCTATCGGGGAAAAGGAAGACAAGGTTCATCACTACCATATACCCGGTGACGATAAGTTTGCTCATTTGGAGACACAGTTTACATTTTCCAATCTGTCTGATCTTGTGGAAGGAGTTCTTGGGAAGACTTACAGGCCAGGTTATGTGAGTCCGGTCAAGAGAGGAGTTCCGATGCCTTTGATGGGTGGTGAAGACAAGTACAAAACTCCATCACTTTTTTCACCTCACTGCAAGGTTTGCAAGTTCCAAACTACCACTCGTCAATCCGGTGGGCTTCCAATTACAACTGGAGAGATTGCTCAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Root_cap

Protein Analysis

458

Amino Acids

47.98

Weight (kDa)

6.56

Isoelectric Point (pI)

4.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Root_cap PF06830 399 - 455 6.9e-28 Root cap
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 983
AccB1I GGYRCC 1 cut(s) 500
AccI GTMKAC 1 cut(s) 930
AccII CGCG 1 cut(s) 1019
AciI CCGC 2 cut(s) 594, 681
AcuI CTGAAG 1 cut(s) 807
AdeI CACNNNGTG 1 cut(s) 500
AfaI GTAC 2 cut(s) 450, 1265
AfiI CCNNNNNNNGG 2 cut(s) 944, 1247
AgsI TTSAA 2 cut(s) 818, 1046
AjnI CCWGG 1 cut(s) 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 1167, 1199
AluBI AGCT 1 cut(s) 69
AluI AGCT 1 cut(s) 69
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 4 cut(s) 24, 742, 877, 1130
Ama87I CYCGRG 1 cut(s) 542
AoxI GGCC 3 cut(s) 79, 1064, 1199
ApeKI GCWGC 1 cut(s) 860
ArsI GACNNNNNNTTYG 2 cut(s) 1106, 1138
Asp700I GAANNNNTTC 2 cut(s) 750, 1179
AspS9I GGNCC 1 cut(s) 79
AsuC2I CCSGG 1 cut(s) 1110
AsuHPI GGTGA 7 cut(s) 254, 695, 932, 983, 1124, 1265, 1278
AvaI CYCGRG 1 cut(s) 542
BanI GGYRCC 1 cut(s) 500
BauI CACGAG 1 cut(s) 798
BbsI GAAGAC 4 cut(s) 981, 1089, 1196, 1263
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 872
BccI CCATC 5 cut(s) 635, 830, 911, 1240, 1282
BcgI CGANNNNNNTGC 2 cut(s) 1106, 1140
BciT130I CCWGG 1 cut(s) 1203
BcnI CCSGG 1 cut(s) 1110
BcoDI GTCTC 4 cut(s) 24, 742, 877, 1130
BisI GCNGC 1 cut(s) 861
BlsI GCNGC 1 cut(s) 862
Bme1390I CCNGG 2 cut(s) 1110, 1203
BmeT110I CYCGRG 1 cut(s) 542
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 3 cut(s) 502, 712, 778
BmrFI CCNGG 2 cut(s) 1110, 1203
BmsI GCATC 3 cut(s) 886, 997, 1227
BpiI GAAGAC 4 cut(s) 981, 1089, 1196, 1263
BpmI CTGGAG 1 cut(s) 1374
BpuMI CCSGG 1 cut(s) 1110
BsaI GGTCTC 1 cut(s) 24
BsaJI CCNNGG 4 cut(s) 57, 82, 706, 714
BsaWI WCCGGW 3 cut(s) 532, 1216, 1331
BsaXI ACNNNNNCTCC 4 cut(s) 784, 814, 1127, 1157
Bsc4I CCNNNNNNNGG 2 cut(s) 944, 1247
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 4 cut(s) 160, 199, 238, 1357
BseBI CCWGG 1 cut(s) 1203
BseDI CCNNGG 4 cut(s) 57, 82, 706, 714
BseGI GGATG 4 cut(s) 889, 922, 933, 969
BseLI CCNNNNNNNGG 2 cut(s) 944, 1247
BseMII CTCAG 1 cut(s) 753
BseNI ACTGG 4 cut(s) 160, 199, 238, 1357
BseRI GAGGAG 1 cut(s) 1242
BseXI GCAGC 1 cut(s) 872
Bsh1236I CGCG 1 cut(s) 1019
BshFI GGCC 3 cut(s) 81, 1066, 1201
BshNI GGYRCC 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 542
BsiSI CCGG 5 cut(s) 117, 533, 1110, 1217, 1332
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 2 cut(s) 944, 1247
BsmAI GTCTC 4 cut(s) 24, 742, 877, 1130
BsmFI GGGAC 1 cut(s) 525
BsnI GGCC 3 cut(s) 81, 1066, 1201
Bso31I GGTCTC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 2 cut(s) 1036, 1165
Bsp19I CCATGG 2 cut(s) 57, 706
BspACI CCGC 2 cut(s) 594, 681
BspANI GGCC 3 cut(s) 81, 1066, 1201
BspCNI CTCAG 1 cut(s) 752
BspFNI CGCG 1 cut(s) 1019
BspLI GGNNCC 3 cut(s) 502, 712, 778
BspT107I GGYRCC 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 24
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 4 cut(s) 160, 199, 238, 1357
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 4 cut(s) 57, 82, 706, 714
BssMI GATC 2 cut(s) 1036, 1165
BssSI CACGAG 1 cut(s) 798
BssT1I CCWWGG 4 cut(s) 57, 82, 706, 714
Bst2BI CACGAG 1 cut(s) 798
Bst2UI CCWGG 1 cut(s) 1203
Bst4CI ACNGT 5 cut(s) 248, 374, 689, 1054, 1143
Bst6I CTCTTC 1 cut(s) 622
BstAPI GCANNNNNTGC 2 cut(s) 500, 1302
BstC8I GCNNGC 2 cut(s) 505, 580
BstDEI CTNAG 2 cut(s) 11, 739
BstDSI CCRYGG 2 cut(s) 57, 706
BstF5I GGATG 4 cut(s) 889, 922, 933, 969
BstFNI CGCG 1 cut(s) 1019
BstKTI GATC 2 cut(s) 1039, 1168
BstMAI GTCTC 4 cut(s) 24, 742, 877, 1130
BstMBI GATC 2 cut(s) 1036, 1165
BstMWI GCNNNNNNNGC 5 cut(s) 234, 500, 657, 1016, 1302
BstNI CCWGG 1 cut(s) 1203
BstNSI RCATGY 1 cut(s) 487
BstSCI CCNGG 2 cut(s) 1108, 1201
BstUI CGCG 1 cut(s) 1019
BstV1I GCAGC 1 cut(s) 872
BstV2I GAAGAC 4 cut(s) 981, 1089, 1196, 1263
BstXI CCANNNNNNTGG 1 cut(s) 89
BsuRI GGCC 3 cut(s) 81, 1066, 1201
BtgI CCRYGG 2 cut(s) 57, 706
BtgZI GCGATG 1 cut(s) 654
BtsCI GGATG 4 cut(s) 889, 922, 933, 969
BtsI GCAGTG 4 cut(s) 127, 205, 244, 1291
BtsIMutI CAGTG 6 cut(s) 127, 192, 205, 244, 433, 1291
Cac8I GCNNGC 2 cut(s) 505, 580
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 79
Csp6I GTAC 2 cut(s) 449, 1264
CviAII CATG 5 cut(s) 58, 484, 707, 810, 832
CviQI GTAC 2 cut(s) 449, 1264
DdeI CTNAG 2 cut(s) 11, 739
DpnI GATC 2 cut(s) 1038, 1167
DpnII GATC 2 cut(s) 1036, 1165
DraIII CACNNNGTG 1 cut(s) 500
DrdI GACNNNNNNGTC 1 cut(s) 983
DseDI GACNNNNNNGTC 1 cut(s) 983
Eam1104I CTCTTC 1 cut(s) 622
EarI CTCTTC 1 cut(s) 622
Eco130I CCWWGG 4 cut(s) 57, 82, 706, 714
Eco147I AGGCCT 1 cut(s) 1066
Eco31I GGTCTC 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 807
Eco88I CYCGRG 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 1201
EcoT14I CCWWGG 4 cut(s) 57, 82, 706, 714
EcoT22I ATGCAT 1 cut(s) 485
ErhI CCWWGG 4 cut(s) 57, 82, 706, 714
FaeI CATG 5 cut(s) 61, 487, 710, 813, 835
FalI AAGNNNNNCTT 4 cut(s) 566, 598, 1167, 1199
FaqI GGGAC 1 cut(s) 525
FatI CATG 5 cut(s) 57, 483, 706, 809, 831
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 1 cut(s) 861
FokI GGATG 4 cut(s) 896, 920, 929, 976
Fsp4HI GCNGC 1 cut(s) 861
GluI GCNGC 1 cut(s) 861
GsuI CTGGAG 1 cut(s) 1374
HaeIII GGCC 3 cut(s) 81, 1066, 1201
HapII CCGG 5 cut(s) 117, 533, 1110, 1217, 1332
Hin1II CATG 5 cut(s) 61, 487, 710, 813, 835
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HinfI GANTC 5 cut(s) 796, 814, 870, 923, 1213
HpaII CCGG 5 cut(s) 117, 533, 1110, 1217, 1332
HphI GGTGA 7 cut(s) 254, 695, 932, 983, 1124, 1265, 1278
Hpy166II GTNNAC 4 cut(s) 530, 589, 931, 1146
Hpy188I TCNGA 5 cut(s) 742, 1036, 1165, 1236, 1373
Hpy188III TCNNGA 4 cut(s) 542, 785, 800, 1222
Hpy8I GTNNAC 4 cut(s) 530, 589, 931, 1146
Hpy99I CGWCG 1 cut(s) 449
HpyAV CCTTC 3 cut(s) 761, 782, 1169
HpyCH4III ACNGT 5 cut(s) 248, 374, 689, 1054, 1143
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 7 cut(s) 483, 489, 618, 764, 860, 1296, 1305
HpyF10VI GCNNNNNNNGC 5 cut(s) 234, 500, 657, 1016, 1302
HpyF3I CTNAG 2 cut(s) 11, 739
HpySE526I ACGT 1 cut(s) 447
Hsp92II CATG 5 cut(s) 61, 487, 710, 813, 835
Kzo9I GATC 2 cut(s) 1036, 1165
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 1 cut(s) 872
LweI GCATC 3 cut(s) 886, 997, 1227
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 7 cut(s) 242, 314, 610, 632, 683, 800, 1112
MalI GATC 2 cut(s) 1038, 1167
MboI GATC 2 cut(s) 1036, 1165
MboII GAAGA 5 cut(s) 639, 986, 1094, 1201, 1268
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 4 cut(s) 439, 1012, 1344, 1367
MlyI GAGTC 4 cut(s) 790, 879, 932, 1222
Mph1103I ATGCAT 1 cut(s) 485
MroXI GAANNNNTTC 2 cut(s) 750, 1179
MslI CAYNNNNRTG 4 cut(s) 29, 56, 535, 759
MspI CCGG 5 cut(s) 117, 533, 1110, 1217, 1332
MspR9I CCNGG 2 cut(s) 1110, 1203
MvaI CCWGG 1 cut(s) 1203
MvnI CGCG 1 cut(s) 1019
MwoI GCNNNNNNNGC 5 cut(s) 234, 500, 657, 1016, 1302
NciI CCSGG 1 cut(s) 1110
NcoI CCATGG 2 cut(s) 57, 706
NdeII GATC 2 cut(s) 1036, 1165
NlaIII CATG 5 cut(s) 61, 487, 710, 813, 835
NlaIV GGNNCC 3 cut(s) 502, 712, 778
NmuCI GTSAC 4 cut(s) 242, 683, 800, 1112
NsiI ATGCAT 1 cut(s) 485
NspI RCATGY 1 cut(s) 487
PceI AGGCCT 1 cut(s) 1066
PdmI GAANNNNTTC 2 cut(s) 750, 1179
PfeI GAWTC 1 cut(s) 814
PkrI GCNGC 1 cut(s) 862
PleI GAGTC 4 cut(s) 790, 878, 931, 1221
PpsI GAGTC 4 cut(s) 790, 878, 931, 1221
Psp6I CCWGG 1 cut(s) 1201
PspGI CCWGG 1 cut(s) 1201
PspN4I GGNNCC 3 cut(s) 502, 712, 778
PspPI GGNCC 1 cut(s) 79
RsaI GTAC 2 cut(s) 450, 1265
RsaNI GTAC 2 cut(s) 449, 1264
RseI CAYNNNNRTG 4 cut(s) 29, 56, 535, 759
SatI GCNGC 1 cut(s) 861
Sau3AI GATC 2 cut(s) 1036, 1165
Sau96I GGNCC 1 cut(s) 79
SchI GAGTC 4 cut(s) 790, 879, 932, 1222
ScrFI CCNGG 2 cut(s) 1110, 1203
SduI GDGCHC 1 cut(s) 51
SfaNI GCATC 3 cut(s) 886, 997, 1227
SmiMI CAYNNNNRTG 4 cut(s) 29, 56, 535, 759
Sse9I AATT 4 cut(s) 439, 1012, 1344, 1367
SseBI AGGCCT 1 cut(s) 1066
SsiI CCGC 2 cut(s) 594, 681
StuI AGGCCT 1 cut(s) 1066
StyD4I CCNGG 2 cut(s) 1108, 1201
StyI CCWWGG 4 cut(s) 57, 82, 706, 714
TaaI ACNGT 5 cut(s) 248, 374, 689, 1054, 1143
TaiI ACGT 1 cut(s) 450
TaqI TCGA 2 cut(s) 670, 993
TaqII GACCGA 1 cut(s) 817
TasI AATT 4 cut(s) 439, 1012, 1344, 1367
TatI WGTACW 1 cut(s) 1263
TfiI GAWTC 1 cut(s) 814
TscAI CASTG 6 cut(s) 127, 199, 205, 244, 433, 1298
TseFI GTSAC 4 cut(s) 242, 683, 800, 1112
TseI GCWGC 1 cut(s) 860
Tsp45I GTSAC 4 cut(s) 242, 683, 800, 1112
TspDTI ATGAA 4 cut(s) 743, 760, 980, 1082
TspGWI ACGGA 7 cut(s) 144, 201, 228, 279, 285, 333, 429
TspRI CASTG 6 cut(s) 127, 199, 205, 244, 433, 1298
XceI RCATGY 1 cut(s) 487
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 930
XmnI GAANNNNTTC 2 cut(s) 750, 1179
Zsp2I ATGCAT 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.