Rorug05G0324000

Root cap

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002778
Physical Location & Seq
Reverse (-)
38877550 .. 38882235
4686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0324000.1

Sequence Viewer

Length: 1344 bp
ATGACAGAGCTCGTCCTCCATTCTCGTCCTGACCATTTCTCTTCATCTCCAACACCCACCACCTCTTCTTCTTCTTCTTCTTCTTCTCCACCACCCTCCTTAACCTCCGCACCGCCGTCTTCTTTAACCTGCGCACCCCCGAGGGACGAGTCTTGGGTGACGGACTGTGACGCCCGGCAGCAGCTGTCCCTGTTGGCATTGCTGGTCACGTTTTTCAGGAAGTCTTTGAGTCATTGCAAGAGGGAAAGGAGGGAGCTTTGTGCCATGGAGATCGGCTGGCCTACCAATGTGCGCCACGTGGCACATGTCACCTTTGACAGGTTCAATGGCTTCTTGGGTTTGCCTGTTGAGTTTGAGCCTGAACTTCCCAGTAGGCCTCCGAGTGCAAGTCGAACGGTTTTTGGAGTGTCTACCAAATCCATGCAGTTGTCGTATGACTCTAGAGGGAATAGTGTGCCGACAATTCTCTTGCTTATGCAAGGGCGGTTGTATGCTGAAGGAGGTCTACAGGTAGAAGGGATATTCAGAATTAATGGAGAAAACAGTCAAGAAGAGCATATAAGAGGCCAATTAAACAGGGGAGATGTACCAGAAGGCATTGACATACACTGCTTGGCAGGGCTGATCAAGGCTTGGTTTAGGGAACTCCCAGTTGGGCTTCTGGATTCTCTAGCCCCTGAGCAGGTAATGCAGTGCCAGACAGAAGATGACTGCTTAGAGCTTGTGAGGCTTCTACCCTCTACCGAAGTTGCTCTATTGGATTGGGCGGTCAACCTTATGGCTGATGTTGTCGAACACGAACATCTAAACAAGATGAATGCGCGCAACATTGCCATGGTTTTTGCACCGAACATGACTCAGATGGCAGATCCTTTGACTGCACTGATGTATGCTGTTCAAGTGATGAACTTCCTCAAGATTCTTATTCTGAAGACACTGCGAGAAAGGAAAGGAAATGTGGTAGAGGCCCGTGTCTTTTGTGTAGAACCTGCTGATGAGAATGGACACCAGAGCTGTTCGCAATCATGCCGTGAAGATGTTGTCGAAGAGAGCCTGGTTGAGAACCCAATTCCTAATGGCAGGGACTGTTGTGGTACTTCTGATCAAGTTGACACTTTGGGTAATGAAATGGAAGCTGTAAACACCATGACAATTGAAGTTCAAGGGGACTCTGGGAAGAGCGAAACTGATCAATTGAATAATTCAACTTTCAAAATGAGACCTGAAAGAATAAGTGATCAGCAAGCTGCAGTATGGGGAACAGGGCCCGTTGAGAAGGCTAGGAAAATCAGCAACCTGAGTCGCATAGATTCAAGGAGGGAGCTAATTGAAGCCTGGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Root_cap

Protein Analysis

447

Amino Acids

49.68

Weight (kDa)

5.13

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 167 - 287 3.3e-23 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 133
Acc36I ACCTGC 3 cut(s) 137, 673, 997
AccI GTMKAC 2 cut(s) 410, 505
AccII CGCG 1 cut(s) 823
AciI CCGC 5 cut(s) 108, 113, 484, 767, 1339
AclWI GGATC 1 cut(s) 863
AcuI CTGAAG 2 cut(s) 516, 950
AcvI CACGTG 1 cut(s) 298
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 2 cut(s) 588, 1096
AfiI CCNNNNNNNGG 2 cut(s) 318, 682
AflIII ACRYGT 1 cut(s) 304
AgsI TTSAA 9 cut(s) 325, 899, 1157, 1163, 1198, 1206, 1213, 1314, 1331
AjnI CCWGG 2 cut(s) 1053, 1334
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 8 cut(s) 10, 184, 256, 721, 1014, 1136, 1247, 1324
AluI AGCT 8 cut(s) 10, 184, 256, 721, 1014, 1136, 1247, 1324
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 1 cut(s) 1213
AlwI GGATC 1 cut(s) 863
AlwNI CAGNNNCTG 2 cut(s) 184, 1086
Ama87I CYCGRG 1 cut(s) 139
AoxI GGCC 5 cut(s) 278, 374, 565, 966, 1265
ApaI GGGCCC 1 cut(s) 1269
ApeKI GCWGC 3 cut(s) 178, 181, 1247
AseI ATTAAT 1 cut(s) 531
AspLEI GCGC 4 cut(s) 134, 294, 823, 825
AspS9I GGNCC 3 cut(s) 967, 1265, 1266
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 2 cut(s) 169, 301
AvaI CYCGRG 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 1269
BanII GRGCYC 2 cut(s) 12, 1269
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BbrPI CACGTG 1 cut(s) 298
BbsI GAAGAC 2 cut(s) 111, 938
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 3 cut(s) 190, 193, 1234
BccI CCATC 1 cut(s) 856
BceAI ACGGC 2 cut(s) 100, 1014
BciT130I CCWGG 2 cut(s) 1055, 1336
BclI TGATCA 4 cut(s) 624, 1102, 1189, 1237
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 1213
BfaI CTAG 3 cut(s) 441, 671, 1281
BfmI CTRYAG 2 cut(s) 506, 1248
BfuAI ACCTGC 3 cut(s) 137, 673, 997
BisI GCNGC 3 cut(s) 179, 182, 1248
BlsI GCNGC 3 cut(s) 180, 183, 1249
Bme1390I CCNGG 3 cut(s) 175, 1055, 1336
BmeT110I CYCGRG 1 cut(s) 139
BmgT120I GGNCC 3 cut(s) 967, 1265, 1266
BmiI GGNNCC 1 cut(s) 1267
BmrFI CCNGG 3 cut(s) 175, 1055, 1336
BmrI ACTGGG 2 cut(s) 363, 644
BmuI ACTGGG 2 cut(s) 363, 644
BpiI GAAGAC 2 cut(s) 111, 938
Bpu10I CCTNAGC 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 175
BsaAI YACGTR 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 1213
BsaJI CCNNGG 3 cut(s) 140, 264, 834
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 682
Bse1I ACTGG 2 cut(s) 369, 650
Bse3DI GCAATG 3 cut(s) 197, 232, 828
BseBI CCWGG 2 cut(s) 1055, 1336
BseDI CCNNGG 3 cut(s) 140, 264, 834
BseLI CCNNNNNNNGG 2 cut(s) 318, 682
BseMI GCAATG 3 cut(s) 197, 232, 828
BseMII CTCAG 3 cut(s) 669, 872, 1289
BseNI ACTGG 2 cut(s) 369, 650
BsePI GCGCGC 1 cut(s) 821
BseSI GKGCMC 1 cut(s) 1269
BseXI GCAGC 3 cut(s) 190, 193, 1234
BsgI GTGCAG 1 cut(s) 864
Bsh1236I CGCG 1 cut(s) 823
BshFI GGCC 5 cut(s) 280, 376, 567, 968, 1267
BsiHKAI GWGCWC 1 cut(s) 12
BsiHKCI CYCGRG 1 cut(s) 139
BsiSI CCGG 1 cut(s) 175
BslFI GGGAC 4 cut(s) 158, 172, 1097, 1181
BslI CCNNNNNNNGG 2 cut(s) 318, 682
BsmAI GTCTC 1 cut(s) 1213
BsmFI GGGAC 4 cut(s) 158, 172, 1097, 1181
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 5 cut(s) 280, 376, 567, 968, 1267
Bso31I GGTCTC 1 cut(s) 1213
BsoBI CYCGRG 1 cut(s) 139
Bsp120I GGGCCC 1 cut(s) 1265
Bsp1286I GDGCHC 2 cut(s) 12, 1269
Bsp143I GATC 6 cut(s) 270, 624, 868, 1102, 1189, 1237
Bsp19I CCATGG 2 cut(s) 264, 834
BspACI CCGC 5 cut(s) 108, 113, 484, 767, 1339
BspANI GGCC 5 cut(s) 280, 376, 567, 968, 1267
BspCNI CTCAG 3 cut(s) 670, 871, 1290
BspFNI CGCG 1 cut(s) 823
BspLI GGNNCC 1 cut(s) 1267
BspMAI CTGCAG 1 cut(s) 1252
BspMI ACCTGC 3 cut(s) 137, 673, 997
BspPI GGATC 1 cut(s) 863
BspQI GCTCTTC 2 cut(s) 546, 1172
BspTNI GGTCTC 1 cut(s) 1213
BsrDI GCAATG 3 cut(s) 197, 232, 828
BsrI ACTGG 2 cut(s) 369, 650
BssECI CCNNGG 3 cut(s) 140, 264, 834
BssHII GCGCGC 1 cut(s) 821
BssMI GATC 6 cut(s) 270, 624, 868, 1102, 1189, 1237
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 2 cut(s) 264, 834
Bst2UI CCWGG 2 cut(s) 1055, 1336
Bst4CI ACNGT 4 cut(s) 167, 397, 545, 1088
Bst6I CTCTTC 5 cut(s) 46, 70, 546, 1041, 1172
BstACI GRCGYC 1 cut(s) 171
BstAPI GCANNNNNTGC 1 cut(s) 688
BstBAI YACGTR 1 cut(s) 298
BstC8I GCNNGC 3 cut(s) 278, 823, 1245
BstDEI CTNAG 4 cut(s) 678, 715, 858, 1298
BstDSI CCRYGG 2 cut(s) 264, 834
BstENI CCTNNNNNAGG 1 cut(s) 316
BstFNI CGCG 1 cut(s) 823
BstHHI GCGC 4 cut(s) 134, 294, 823, 825
BstKTI GATC 6 cut(s) 273, 627, 871, 1105, 1192, 1240
BstMAI GTCTC 1 cut(s) 1213
BstMBI GATC 6 cut(s) 270, 624, 868, 1102, 1189, 1237
BstMWI GCNNNNNNNGC 2 cut(s) 688, 727
BstNI CCWGG 2 cut(s) 1055, 1336
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 3 cut(s) 173, 1053, 1334
BstSFI CTRYAG 2 cut(s) 506, 1248
BstSLI GKGCMC 1 cut(s) 1269
BstUI CGCG 1 cut(s) 823
BstV1I GCAGC 3 cut(s) 190, 193, 1234
BstV2I GAAGAC 2 cut(s) 111, 938
BstX2I RGATCY 1 cut(s) 868
BstYI RGATCY 1 cut(s) 868
BsuRI GGCC 5 cut(s) 280, 376, 567, 968, 1267
BtgI CCRYGG 2 cut(s) 264, 834
BtsI GCAGTG 3 cut(s) 607, 698, 935
BtsIMutI CAGTG 4 cut(s) 607, 698, 881, 935
BveI ACCTGC 3 cut(s) 137, 673, 997
Cac8I GCNNGC 3 cut(s) 278, 823, 1245
CaiI CAGNNNCTG 2 cut(s) 184, 1086
CfoI GCGC 4 cut(s) 134, 294, 823, 825
Cfr13I GGNCC 3 cut(s) 967, 1265, 1266
CseI GACGC 1 cut(s) 179
Csp6I GTAC 2 cut(s) 587, 1095
CviAII CATG 7 cut(s) 265, 305, 421, 835, 853, 1026, 1147
CviQI GTAC 2 cut(s) 587, 1095
DdeI CTNAG 4 cut(s) 678, 715, 858, 1298
DpnI GATC 6 cut(s) 272, 626, 870, 1104, 1191, 1239
DpnII GATC 6 cut(s) 270, 624, 868, 1102, 1189, 1237
Eam1104I CTCTTC 5 cut(s) 46, 70, 546, 1041, 1172
EarI CTCTTC 5 cut(s) 46, 70, 546, 1041, 1172
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 2 cut(s) 264, 834
Eco147I AGGCCT 1 cut(s) 376
Eco24I GRGCYC 2 cut(s) 12, 1269
Eco31I GGTCTC 1 cut(s) 1213
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 2 cut(s) 516, 950
Eco72I CACGTG 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 316
EcoO109I RGGNCCY 1 cut(s) 1265
EcoRII CCWGG 2 cut(s) 1053, 1334
EcoT14I CCWWGG 2 cut(s) 264, 834
EcoT38I GRGCYC 2 cut(s) 12, 1269
ErhI CCWWGG 2 cut(s) 264, 834
FaeI CATG 7 cut(s) 268, 308, 424, 838, 856, 1029, 1150
FaqI GGGAC 4 cut(s) 158, 172, 1097, 1181
FatI CATG 7 cut(s) 264, 304, 420, 834, 852, 1025, 1146
FbaI TGATCA 4 cut(s) 624, 1102, 1189, 1237
FblI GTMKAC 2 cut(s) 410, 505
Fnu4HI GCNGC 3 cut(s) 179, 182, 1248
FriOI GRGCYC 2 cut(s) 12, 1269
Fsp4HI GCNGC 3 cut(s) 179, 182, 1248
FspBI CTAG 3 cut(s) 441, 671, 1281
FspI TGCGCA 1 cut(s) 133
GlaI GCGC 4 cut(s) 133, 293, 822, 824
GluI GCNGC 3 cut(s) 179, 182, 1248
HaeIII GGCC 5 cut(s) 280, 376, 567, 968, 1267
HapII CCGG 1 cut(s) 175
HgaI GACGC 1 cut(s) 179
HhaI GCGC 4 cut(s) 134, 294, 823, 825
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 7 cut(s) 268, 308, 424, 838, 856, 1029, 1150
Hin6I GCGC 4 cut(s) 132, 292, 821, 823
HinP1I GCGC 4 cut(s) 132, 292, 821, 823
HincII GTYRAC 2 cut(s) 772, 1111
HindII GTYRAC 2 cut(s) 772, 1111
HinfI GANTC 9 cut(s) 149, 229, 437, 665, 856, 919, 1169, 1300, 1310
HpaII CCGG 1 cut(s) 175
HphI GGTGA 2 cut(s) 169, 301
Hpy166II GTNNAC 5 cut(s) 411, 506, 772, 1111, 1141
Hpy188I TCNGA 5 cut(s) 381, 527, 861, 930, 1102
Hpy188III TCNNGA 6 cut(s) 29, 217, 441, 548, 662, 916
Hpy8I GTNNAC 5 cut(s) 411, 506, 772, 1111, 1141
HpyAV CCTTC 4 cut(s) 491, 509, 587, 1270
HpyCH4III ACNGT 4 cut(s) 167, 397, 545, 1088
HpyCH4IV ACGT 2 cut(s) 209, 297
HpyCH4V TGCA 8 cut(s) 237, 386, 424, 478, 691, 845, 881, 1250
HpyF10VI GCNNNNNNNGC 2 cut(s) 688, 727
HpyF3I CTNAG 4 cut(s) 678, 715, 858, 1298
HpySE526I ACGT 2 cut(s) 209, 297
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 7 cut(s) 268, 308, 424, 838, 856, 1029, 1150
HspAI GCGC 4 cut(s) 132, 292, 821, 823
Ksp22I TGATCA 4 cut(s) 624, 1102, 1189, 1237
Kzo9I GATC 6 cut(s) 270, 624, 868, 1102, 1189, 1237
LguI GCTCTTC 2 cut(s) 546, 1172
LmnI GCTCC 2 cut(s) 253, 1321
Lsp1109I GCAGC 3 cut(s) 190, 193, 1234
MaeI CTAG 3 cut(s) 441, 671, 1281
MaeII ACGT 2 cut(s) 209, 297
MaeIII GTNAC 4 cut(s) 157, 167, 205, 307
MalI GATC 6 cut(s) 272, 626, 870, 1104, 1191, 1239
MboI GATC 6 cut(s) 270, 624, 868, 1102, 1189, 1237
MfeI CAATTG 2 cut(s) 1152, 1193
MflI RGATCY 1 cut(s) 868
MhlI GDGCHC 2 cut(s) 12, 1269
MluCI AATT 8 cut(s) 462, 528, 569, 1068, 1152, 1193, 1201, 1326
MlyI GAGTC 6 cut(s) 158, 238, 431, 850, 1163, 1309
MmeI TCCRAC 1 cut(s) 74
MseI TTAA 4 cut(s) 101, 125, 531, 572
MslI CAYNNNNRTG 1 cut(s) 833
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 1 cut(s) 175
MspR9I CCNGG 3 cut(s) 175, 1055, 1336
MunI CAATTG 2 cut(s) 1152, 1193
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 2 cut(s) 1055, 1336
MvnI CGCG 1 cut(s) 823
MwoI GCNNNNNNNGC 2 cut(s) 688, 727
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 2 cut(s) 264, 834
NdeII GATC 6 cut(s) 270, 624, 868, 1102, 1189, 1237
NlaIII CATG 7 cut(s) 268, 308, 424, 838, 856, 1029, 1150
NlaIV GGNNCC 1 cut(s) 1267
NmuCI GTSAC 4 cut(s) 157, 167, 205, 307
NsbI TGCGCA 1 cut(s) 133
NspI RCATGY 1 cut(s) 308
PauI GCGCGC 1 cut(s) 821
PceI AGGCCT 1 cut(s) 376
PciI ACATGT 1 cut(s) 304
PciSI GCTCTTC 2 cut(s) 546, 1172
PctI GAATGC 1 cut(s) 823
PfeI GAWTC 3 cut(s) 665, 919, 1310
PkrI GCNGC 3 cut(s) 180, 183, 1249
PleI GAGTC 6 cut(s) 157, 237, 431, 850, 1163, 1308
PmaCI CACGTG 1 cut(s) 298
PmlI CACGTG 1 cut(s) 298
PpsI GAGTC 6 cut(s) 157, 237, 431, 850, 1163, 1308
Ppu21I YACGTR 1 cut(s) 298
PscI ACATGT 1 cut(s) 304
PshBI ATTAAT 1 cut(s) 531
Psp124BI GAGCTC 1 cut(s) 12
Psp6I CCWGG 2 cut(s) 1053, 1334
PspCI CACGTG 1 cut(s) 298
PspGI CCWGG 2 cut(s) 1053, 1334
PspN4I GGNNCC 1 cut(s) 1267
PspOMI GGGCCC 1 cut(s) 1265
PspPI GGNCC 3 cut(s) 967, 1265, 1266
PstI CTGCAG 1 cut(s) 1252
PstNI CAGNNNCTG 2 cut(s) 184, 1086
PsuI RGATCY 1 cut(s) 868
PteI GCGCGC 1 cut(s) 821
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 2 cut(s) 588, 1096
RsaNI GTAC 2 cut(s) 587, 1095
RseI CAYNNNNRTG 1 cut(s) 833
SacI GAGCTC 1 cut(s) 12
SapI GCTCTTC 2 cut(s) 546, 1172
SaqAI TTAA 4 cut(s) 101, 125, 531, 572
SatI GCNGC 3 cut(s) 179, 182, 1248
Sau3AI GATC 6 cut(s) 270, 624, 868, 1102, 1189, 1237
Sau96I GGNCC 3 cut(s) 967, 1265, 1266
SchI GAGTC 6 cut(s) 158, 238, 431, 850, 1163, 1309
ScrFI CCNGG 3 cut(s) 175, 1055, 1336
SduI GDGCHC 2 cut(s) 12, 1269
SfcI CTRYAG 2 cut(s) 506, 1248
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 1 cut(s) 914
SmoI CTYRAG 1 cut(s) 914
Sse9I AATT 8 cut(s) 462, 528, 569, 1068, 1152, 1193, 1201, 1326
SseBI AGGCCT 1 cut(s) 376
SsiI CCGC 5 cut(s) 108, 113, 484, 767, 1339
SspMI CTAG 3 cut(s) 441, 671, 1281
SstI GAGCTC 1 cut(s) 12
StuI AGGCCT 1 cut(s) 376
StyD4I CCNGG 3 cut(s) 173, 1053, 1334
StyI CCWWGG 2 cut(s) 264, 834
TaaI ACNGT 4 cut(s) 167, 397, 545, 1088
TaiI ACGT 2 cut(s) 212, 300
TaqI TCGA 3 cut(s) 391, 792, 1044
TasI AATT 8 cut(s) 462, 528, 569, 1068, 1152, 1193, 1201, 1326
TfiI GAWTC 3 cut(s) 665, 919, 1310
Tru1I TTAA 4 cut(s) 101, 125, 531, 572
Tru9I TTAA 4 cut(s) 101, 125, 531, 572
TscAI CASTG 4 cut(s) 614, 698, 888, 942
TseFI GTSAC 4 cut(s) 157, 167, 205, 307
TseI GCWGC 3 cut(s) 178, 181, 1247
Tsp45I GTSAC 4 cut(s) 157, 167, 205, 307
TspDTI ATGAA 4 cut(s) 33, 830, 920, 1140
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 4 cut(s) 614, 698, 888, 942
VspI ATTAAT 1 cut(s) 531
XagI CCTNNNNNAGG 1 cut(s) 316
XbaI TCTAGA 1 cut(s) 440
XceI RCATGY 1 cut(s) 308
XmiI GTMKAC 2 cut(s) 410, 505
XspI CTAG 3 cut(s) 441, 671, 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.