FvH4_3g33160

Protein disulfide isomerase-like 5-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008513
Physical Location & Seq
Reverse (-)
28572538 .. 28575406
2869 bp
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UTR
Exon/CDS
Intron
FvH4_3g33160.t2

Sequence Viewer

Length: 441 bp
ATGAAAACCCAGTTAGCAGCTTCTCTAGTTCTCATGTCCCTTCTTCTTCTCCTAACAATCCACACCAGAGCTGAGGTTATAACCTTAACCGCTGACACCTTCTCCGATAAGGTGAAGGAGAAAGACACTGCGTGGTTTGTGAAGTTCTGTGTGCCATGGTGTAAACATTGTAAGAAGTTGGGGTCATTGTGGGAGGATTTCGGAAAGGCGGTGGAAAGTGAAGATGAGATAGAGGTTGGGGAAGTTGATTGCAGTACGAGTAAACCGGTGTGTTCCAAAGTTGATATTCATTCTTATCCTACGTTTAAGCTATTCTTTGATGGAGAAGAAGTTGCAAAATATCAAGGGCCAAGGGATGTTGAATCACTCAAGACTTTTGTCTTAGACCAAGCTGAAGAAGCAGCAAGAAAGGCACATCTTGGCAGTGATAAAGAGTTGTGA

Protein Analysis

147

Amino Acids

16.42

Weight (kDa)

5.24

Isoelectric Point (pI)

22.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 26 - 127 6.3e-24 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 80
AciI CCGC 2 cut(s) 90, 209
AcuI CTGAAG 1 cut(s) 414
AdeI CACNNNGTG 1 cut(s) 132
AfaI GTAC 1 cut(s) 256
AgeI ACCGGT 1 cut(s) 265
AgsI TTSAA 1 cut(s) 362
AluBI AGCT 4 cut(s) 20, 71, 310, 392
AluI AGCT 4 cut(s) 20, 71, 310, 392
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 2 cut(s) 17, 401
AsiGI ACCGGT 1 cut(s) 265
AspS9I GGNCC 1 cut(s) 347
AsuHPI GGTGA 1 cut(s) 124
BbvCI CCTCAGC 1 cut(s) 72
BbvI GCAGC 2 cut(s) 29, 413
BccI CCATC 1 cut(s) 314
BfaI CTAG 1 cut(s) 26
BisI GCNGC 2 cut(s) 18, 402
BlsI GCNGC 2 cut(s) 19, 403
BmgT120I GGNCC 1 cut(s) 347
BmrI ACTGGG 1 cut(s) 4
BmuI ACTGGG 1 cut(s) 4
BoxI GACNNNNGTC 1 cut(s) 377
Bpu10I CCTNAGC 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 353
BsaJI CCNNGG 2 cut(s) 155, 350
BsaWI WCCGGW 1 cut(s) 265
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 315, 345
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 10
BseDI CCNNGG 2 cut(s) 155, 350
BseGI GGATG 1 cut(s) 361
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 10
BseXI GCAGC 2 cut(s) 29, 413
BshFI GGCC 1 cut(s) 349
BshTI ACCGGT 1 cut(s) 265
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 1 cut(s) 349
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 2 cut(s) 90, 209
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 1 cut(s) 64
BsrFI RCCGGY 1 cut(s) 265
BsrI ACTGG 1 cut(s) 10
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 2 cut(s) 155, 350
BssT1I CCWWGG 2 cut(s) 155, 350
BstDEI CTNAG 2 cut(s) 72, 382
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 1 cut(s) 361
BstMWI GCNNNNNNNGC 2 cut(s) 398, 410
BstPAI GACNNNNGTC 1 cut(s) 377
BstV1I GCAGC 2 cut(s) 29, 413
BsuRI GGCC 1 cut(s) 349
BtgI CCRYGG 1 cut(s) 155
BtsCI GGATG 1 cut(s) 361
BtsI GCAGTG 2 cut(s) 126, 430
BtsIMutI CAGTG 2 cut(s) 126, 430
Cfr10I RCCGGY 1 cut(s) 265
Cfr13I GGNCC 1 cut(s) 347
Csp6I GTAC 1 cut(s) 255
CspAI ACCGGT 1 cut(s) 265
CviAII CATG 2 cut(s) 34, 156
CviJI RGCY 5 cut(s) 20, 71, 310, 349, 392
CviKI_1 RGCY 5 cut(s) 20, 71, 310, 349, 392
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 2 cut(s) 72, 382
DraIII CACNNNGTG 1 cut(s) 132
Eco130I CCWWGG 2 cut(s) 155, 350
Eco57I CTGAAG 1 cut(s) 414
EcoT14I CCWWGG 2 cut(s) 155, 350
ErhI CCWWGG 2 cut(s) 155, 350
FaeI CATG 2 cut(s) 37, 159
FaiI YATR 3 cut(s) 35, 80, 157
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 1 cut(s) 22
FatI CATG 2 cut(s) 33, 155
Fnu4HI GCNGC 2 cut(s) 18, 402
FokI GGATG 1 cut(s) 368
Fsp4HI GCNGC 2 cut(s) 18, 402
FspBI CTAG 1 cut(s) 26
GluI GCNGC 2 cut(s) 18, 402
HaeIII GGCC 1 cut(s) 349
HapII CCGG 1 cut(s) 266
Hin1II CATG 2 cut(s) 37, 159
HinfI GANTC 1 cut(s) 362
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 2 cut(s) 164, 263
Hpy188I TCNGA 2 cut(s) 106, 203
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 2 cut(s) 164, 263
HpyAV CCTTC 3 cut(s) 50, 109, 109
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 2 cut(s) 252, 335
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 410
HpyF3I CTNAG 2 cut(s) 72, 382
HpySE526I ACGT 1 cut(s) 302
Hsp92II CATG 2 cut(s) 37, 159
LpnPI CCDG 3 cut(s) 23, 79, 279
Lsp1109I GCAGC 2 cut(s) 29, 413
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 302
MboII GAAGA 5 cut(s) 35, 38, 233, 338, 407
MnlI CCTC 3 cut(s) 67, 187, 226
MseI TTAA 2 cut(s) 86, 306
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 398, 410
NcoI CCATGG 1 cut(s) 155
NlaIII CATG 2 cut(s) 37, 159
PfeI GAWTC 1 cut(s) 362
PinAI ACCGGT 1 cut(s) 265
PkrI GCNGC 2 cut(s) 19, 403
PshAI GACNNNNGTC 1 cut(s) 377
PsiI TTATAA 1 cut(s) 80
PspPI GGNCC 1 cut(s) 347
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 86, 306
SatI GCNGC 2 cut(s) 18, 402
Sau96I GGNCC 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
SsiI CCGC 2 cut(s) 90, 209
SspMI CTAG 1 cut(s) 26
StyI CCWWGG 2 cut(s) 155, 350
TaiI ACGT 1 cut(s) 305
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 2 cut(s) 86, 306
Tru9I TTAA 2 cut(s) 86, 306
TscAI CASTG 2 cut(s) 133, 430
TseI GCWGC 2 cut(s) 17, 401
TspDTI ATGAA 2 cut(s) 17, 278
TspRI CASTG 2 cut(s) 133, 430
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.