MD03G1105000.v1.1

Protein disulfide isomerase-like 5-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008513
Physical Location & Seq
Reverse (-)
8989116 .. 8992084
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1105000.v1.1.491

Sequence Viewer

Length: 639 bp
ATGAAAACCCACTCTGCTTCTCTAGTTTTCGTCTCAGTTCTTCTCTTTCTTCTTCCAATCCTAACAACCCATGCCGAAGCTGAGGTTATAACCCTAACCGCCGACACCTTCTCGGACAAGGTGAAGGAGAGAGACACGGCATGGTTTGTGAAATTCTGTGTACCGTGGTGTAAACATTGTAAGAACATGGGTACGTTGTGGGAGGACCTTGGGAAGACAGTGGAAGGCGAAGACGAAATAGAGGTTGGGGAAGTTGATTGCAGTACGAGTAAACCCGTATGTTCCAAAGTTGACATTCATTCATATCCTACGTTTAAAGTATTCTATGATGGTGAAGAAGTCGCAAAATATCAAGGGCCGAGGGATGTTGACTCACTTAAAAACTTTGTCTTAGAAGAAGCCGAAAAGGCAGCAACAAGGGCACAGCTTGACAATGATAAAGAGTTATGTGACTTGCGTGTTGAACCCGCATTAGCACACATGGTGACAGACGCCCAGTATATGCTTCTATGGACATCAGCTCAGCTTCAGCTTCAGCAGTTGGGTTCGGGCACAACAATTTTGACTCGGGATCGCGACTCCAGTGGGGCAAATACCAACTACAACTCATACATGGGCAACAGCAGCTTGTATTCATGA

Protein Analysis

213

Amino Acids

23.74

Weight (kDa)

4.81

Isoelectric Point (pI)

28.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 29 - 130 4.7e-24 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 89
AccII CGCG 1 cut(s) 576
AciI CCGC 2 cut(s) 99, 468
AclWI GGATC 1 cut(s) 579
AcsI RAATTY 1 cut(s) 152
AcuI CTGAAG 2 cut(s) 512, 518
AcyI GRCGYC 1 cut(s) 492
AdeI CACNNNGTG 1 cut(s) 484
AfaI GTAC 3 cut(s) 162, 193, 265
AgsI TTSAA 1 cut(s) 464
AjuI GAANNNNNNNTTGG 2 cut(s) 228, 260
AluBI AGCT 6 cut(s) 80, 427, 521, 526, 532, 627
AluI AGCT 6 cut(s) 80, 427, 521, 526, 532, 627
Alw26I GTCTC 2 cut(s) 37, 126
AlwI GGATC 1 cut(s) 579
Ama87I CYCGRG 1 cut(s) 567
AoxI GGCC 1 cut(s) 356
ApeKI GCWGC 2 cut(s) 410, 624
ApoI RAATTY 1 cut(s) 152
AspS9I GGNCC 2 cut(s) 205, 356
AsuHPI GGTGA 3 cut(s) 133, 344, 496
AvaI CYCGRG 1 cut(s) 567
AvaII GGWCC 1 cut(s) 205
BaeGI GKGCMC 2 cut(s) 424, 554
BbsI GAAGAC 2 cut(s) 221, 237
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 422
BccI CCATC 1 cut(s) 323
BceAI ACGGC 1 cut(s) 153
BcoDI GTCTC 2 cut(s) 37, 126
BfaI CTAG 1 cut(s) 23
BglI GCCNNNNNGGC 1 cut(s) 407
BisI GCNGC 2 cut(s) 411, 625
BlpI GCTNAGC 1 cut(s) 522
BlsI GCNGC 2 cut(s) 412, 626
Bme18I GGWCC 1 cut(s) 205
BmeT110I CYCGRG 1 cut(s) 567
BmgT120I GGNCC 2 cut(s) 205, 356
BmrI ACTGGG 1 cut(s) 490
BmuI ACTGGG 1 cut(s) 490
BpiI GAAGAC 2 cut(s) 221, 237
BpmI CTGGAG 1 cut(s) 565
Bpu10I CCTNAGC 1 cut(s) 81
Bpu1102I GCTNAGC 1 cut(s) 522
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 3 cut(s) 164, 208, 359
Bse1I ACTGG 2 cut(s) 496, 582
BseDI CCNNGG 3 cut(s) 164, 208, 359
BseGI GGATG 1 cut(s) 370
BseMII CTCAG 3 cut(s) 48, 72, 536
BseNI ACTGG 2 cut(s) 496, 582
BseSI GKGCMC 2 cut(s) 424, 554
BseXI GCAGC 1 cut(s) 422
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 1 cut(s) 358
BsiHKCI CYCGRG 1 cut(s) 567
BsmAI GTCTC 2 cut(s) 37, 126
BsmBI CGTCTC 1 cut(s) 37
BsnI GGCC 1 cut(s) 358
BsoBI CYCGRG 1 cut(s) 567
Bsp1286I GDGCHC 2 cut(s) 424, 554
Bsp143I GATC 1 cut(s) 571
Bsp1720I GCTNAGC 1 cut(s) 522
Bsp68I TCGCGA 1 cut(s) 576
BspACI CCGC 2 cut(s) 99, 468
BspANI GGCC 1 cut(s) 358
BspCNI CTCAG 3 cut(s) 47, 73, 535
BspFNI CGCG 1 cut(s) 576
BspHI TCATGA 1 cut(s) 635
BspPI GGATC 1 cut(s) 579
BsrI ACTGG 2 cut(s) 496, 582
BssECI CCNNGG 3 cut(s) 164, 208, 359
BssMI GATC 1 cut(s) 571
BssNI GRCGYC 1 cut(s) 492
BssT1I CCWWGG 1 cut(s) 208
Bst4CI ACNGT 2 cut(s) 165, 220
BstACI GRCGYC 1 cut(s) 492
BstDEI CTNAG 4 cut(s) 34, 81, 391, 522
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 1 cut(s) 370
BstFNI CGCG 1 cut(s) 576
BstKTI GATC 1 cut(s) 574
BstMAI GTCTC 2 cut(s) 37, 126
BstMBI GATC 1 cut(s) 571
BstMWI GCNNNNNNNGC 3 cut(s) 407, 419, 624
BstSLI GKGCMC 2 cut(s) 424, 554
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 1 cut(s) 422
BstV2I GAAGAC 2 cut(s) 221, 237
BsuRI GGCC 1 cut(s) 358
BtgI CCRYGG 1 cut(s) 164
BtsCI GGATG 1 cut(s) 370
BtsIMutI CAGTG 2 cut(s) 225, 589
BtuMI TCGCGA 1 cut(s) 576
CciI TCATGA 1 cut(s) 635
Cfr13I GGNCC 2 cut(s) 205, 356
CseI GACGC 1 cut(s) 500
Csp6I GTAC 3 cut(s) 161, 192, 264
CviAII CATG 6 cut(s) 71, 141, 187, 481, 613, 636
CviJI RGCY 8 cut(s) 80, 358, 401, 427, 521, 526, 532, 627
CviKI_1 RGCY 8 cut(s) 80, 358, 401, 427, 521, 526, 532, 627
CviQI GTAC 3 cut(s) 161, 192, 264
DdeI CTNAG 4 cut(s) 34, 81, 391, 522
DpnI GATC 1 cut(s) 573
DpnII GATC 1 cut(s) 571
DraI TTTAAA 1 cut(s) 316
DraIII CACNNNGTG 1 cut(s) 484
Eco130I CCWWGG 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 205
Eco57I CTGAAG 2 cut(s) 512, 518
Eco88I CYCGRG 1 cut(s) 567
EcoO109I RGGNCCY 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 208
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 6 cut(s) 74, 144, 190, 484, 616, 639
FatI CATG 6 cut(s) 70, 140, 186, 480, 612, 635
FauI CCCGC 1 cut(s) 475
Fnu4HI GCNGC 2 cut(s) 411, 625
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 2 cut(s) 411, 625
FspBI CTAG 1 cut(s) 23
GluI GCNGC 2 cut(s) 411, 625
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 1 cut(s) 358
HgaI GACGC 1 cut(s) 500
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 6 cut(s) 74, 144, 190, 484, 616, 639
HincII GTYRAC 2 cut(s) 292, 370
HindII GTYRAC 2 cut(s) 292, 370
HinfI GANTC 3 cut(s) 371, 565, 578
HphI GGTGA 3 cut(s) 133, 344, 496
Hpy166II GTNNAC 5 cut(s) 161, 173, 272, 292, 370
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 3 cut(s) 569, 575, 636
Hpy8I GTNNAC 5 cut(s) 161, 173, 272, 292, 370
HpyAV CCTTC 3 cut(s) 118, 118, 218
HpyCH4III ACNGT 2 cut(s) 165, 220
HpyCH4IV ACGT 2 cut(s) 194, 311
HpyCH4V TGCA 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 3 cut(s) 407, 419, 624
HpyF3I CTNAG 4 cut(s) 34, 81, 391, 522
HpySE526I ACGT 2 cut(s) 194, 311
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 6 cut(s) 74, 144, 190, 484, 616, 639
Kzo9I GATC 1 cut(s) 571
LpnPI CCDG 2 cut(s) 509, 595
Lsp1109I GCAGC 1 cut(s) 422
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 194, 311
MaeIII GTNAC 2 cut(s) 449, 484
MalI GATC 1 cut(s) 573
MboI GATC 1 cut(s) 571
MboII GAAGA 7 cut(s) 32, 41, 44, 226, 242, 347, 407
MhlI GDGCHC 2 cut(s) 424, 554
MluCI AATT 2 cut(s) 152, 558
MlyI GAGTC 3 cut(s) 365, 559, 572
MnlI CCTC 4 cut(s) 76, 196, 235, 354
MseI TTAA 2 cut(s) 315, 378
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 3 cut(s) 407, 419, 624
NdeII GATC 1 cut(s) 571
NlaIII CATG 6 cut(s) 74, 144, 190, 484, 616, 639
NmeAIII GCCGAG 1 cut(s) 384
NmuCI GTSAC 2 cut(s) 449, 484
NruI TCGCGA 1 cut(s) 576
PagI TCATGA 1 cut(s) 635
PkrI GCNGC 2 cut(s) 412, 626
PleI GAGTC 3 cut(s) 365, 559, 572
PpsI GAGTC 3 cut(s) 365, 559, 572
PpuMI RGGWCCY 1 cut(s) 205
PsiI TTATAA 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 205
PspPI GGNCC 2 cut(s) 205, 356
PspPPI RGGWCCY 1 cut(s) 205
RruI TCGCGA 1 cut(s) 576
RsaI GTAC 3 cut(s) 162, 193, 265
RsaNI GTAC 3 cut(s) 161, 192, 264
SaqAI TTAA 2 cut(s) 315, 378
SatI GCNGC 2 cut(s) 411, 625
Sau3AI GATC 1 cut(s) 571
Sau96I GGNCC 2 cut(s) 205, 356
SchI GAGTC 3 cut(s) 365, 559, 572
SduI GDGCHC 2 cut(s) 424, 554
SinI GGWCC 1 cut(s) 205
Sse9I AATT 2 cut(s) 152, 558
SsiI CCGC 2 cut(s) 99, 468
SspMI CTAG 1 cut(s) 23
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 2 cut(s) 165, 220
TaiI ACGT 2 cut(s) 197, 314
TasI AATT 2 cut(s) 152, 558
Tru1I TTAA 2 cut(s) 315, 378
Tru9I TTAA 2 cut(s) 315, 378
TscAI CASTG 2 cut(s) 225, 589
TseFI GTSAC 2 cut(s) 449, 484
TseI GCWGC 2 cut(s) 410, 624
Tsp45I GTSAC 2 cut(s) 449, 484
TspDTI ATGAA 4 cut(s) 17, 287, 291, 624
TspRI CASTG 2 cut(s) 225, 589
VpaK11BI GGWCC 1 cut(s) 205
XapI RAATTY 1 cut(s) 152
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.