Rroxscaffold_1G00020310

Protein disulfide isomerase-like 5-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008513
Physical Location & Seq
Forward (+)
24642647 .. 24646196
3550 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00020310.1

Sequence Viewer

Length: 531 bp
ATGAAAACCCAGTTAGCAGCTTCTCTAATTTTCACGTCCCTTCTTCTTTTCCTTCTCCCAAGTTTAACAATTCATACCAAAGCTGAGGTTATAACCCTAACTGCTGACACCTTCTCCGATAAGGTGAAGGAGAAAGACACTGCATGGTTTGTCAAGTTCTGTGTACCATGGTGTAAACATTGTAAGAACTTGGGGTCATTGTGGGAGGATTTTGGGAAGGCAGTGGAAAACGAAGACGAAATAGAGGTTGGGGAAGTGGATTGCAGTACGAGTAAACCAGTGTGCTCGAAAGTTGATATTCATTCGTATCCTACATTTAAGCTATTCTTTGATGGAGAAGAAGTTGCAAAATATCAAGGGTTCAGCAGGGACCCAGGTGTCGAGCCTGGGTCTGTGATGCGGGTTTGGGGCATCACTGGGTATAATAAATCATTTTGGCCAAGGGATGTTGATTCACTCAAGAGTTTTGTCTTAGACCGGGCTGAAGAAGCAGCAAGAAAGGCACATCTTGGCAGTGATAAAGAGTTGTGA

Protein Analysis

176

Amino Acids

19.82

Weight (kDa)

5.42

Isoelectric Point (pI)

17.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 30 - 121 1.6e-21 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 92
AasI GACNNNNNNGTC 1 cut(s) 377
AciI CCGC 1 cut(s) 400
AcoI YGGCCR 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 504
AfaI GTAC 2 cut(s) 165, 268
AjiI CACGTC 1 cut(s) 36
AjnI CCWGG 2 cut(s) 373, 385
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AluBI AGCT 3 cut(s) 20, 83, 322
AluI AGCT 3 cut(s) 20, 83, 322
Alw21I GWGCWC 1 cut(s) 287
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 2 cut(s) 17, 491
AspS9I GGNCC 1 cut(s) 370
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 1 cut(s) 136
AvaII GGWCC 1 cut(s) 370
BalI TGGCCA 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 287
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 29, 503
BccI CCATC 1 cut(s) 326
BciT130I CCWGG 2 cut(s) 375, 387
BciVI GTATCC 1 cut(s) 318
BcnI CCSGG 1 cut(s) 479
BfuI GTATCC 1 cut(s) 318
BisI GCNGC 2 cut(s) 18, 492
BlsI GCNGC 2 cut(s) 19, 493
Bme1390I CCNGG 3 cut(s) 375, 387, 479
Bme18I GGWCC 1 cut(s) 370
BmgBI CACGTC 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 2 cut(s) 371, 372
BmrFI CCNGG 3 cut(s) 375, 387, 479
BmrI ACTGGG 2 cut(s) 4, 426
BmsI GCATC 2 cut(s) 387, 420
BmuI ACTGGG 2 cut(s) 4, 426
BpiI GAAGAC 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 479
BsaJI CCNNGG 4 cut(s) 167, 373, 386, 440
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 327, 357
Bse1I ACTGG 3 cut(s) 10, 278, 421
BseBI CCWGG 2 cut(s) 375, 387
BseDI CCNNGG 4 cut(s) 167, 373, 386, 440
BseGI GGATG 1 cut(s) 451
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 3 cut(s) 10, 278, 421
BseXI GCAGC 2 cut(s) 29, 503
BshFI GGCC 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 287
BsiSI CCGG 1 cut(s) 478
BslFI GGGAC 2 cut(s) 22, 383
BsmFI GGGAC 2 cut(s) 22, 383
BsnI GGCC 1 cut(s) 439
Bsp1286I GDGCHC 1 cut(s) 287
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 1 cut(s) 400
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 371, 372
BsrI ACTGG 3 cut(s) 10, 278, 421
BssECI CCNNGG 4 cut(s) 167, 373, 386, 440
BssT1I CCWWGG 2 cut(s) 167, 440
Bst2UI CCWGG 2 cut(s) 375, 387
BstDEI CTNAG 2 cut(s) 84, 472
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 451
BstMWI GCNNNNNNNGC 2 cut(s) 488, 500
BstNI CCWGG 2 cut(s) 375, 387
BstSCI CCNGG 3 cut(s) 373, 385, 477
BstV1I GCAGC 2 cut(s) 29, 503
BstV2I GAAGAC 1 cut(s) 240
BsuI GTATCC 1 cut(s) 318
BsuRI GGCC 1 cut(s) 439
BtgI CCRYGG 1 cut(s) 167
BtrI CACGTC 1 cut(s) 36
BtsCI GGATG 1 cut(s) 451
BtsI GCAGTG 3 cut(s) 138, 228, 520
BtsIMutI CAGTG 5 cut(s) 138, 228, 285, 414, 520
Cfr13I GGNCC 1 cut(s) 370
Csp6I GTAC 2 cut(s) 164, 267
CviAII CATG 2 cut(s) 144, 168
CviJI RGCY 6 cut(s) 20, 83, 322, 385, 439, 482
CviKI_1 RGCY 6 cut(s) 20, 83, 322, 385, 439, 482
CviQI GTAC 2 cut(s) 164, 267
DdeI CTNAG 2 cut(s) 84, 472
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 437
Eco130I CCWWGG 2 cut(s) 167, 440
Eco47I GGWCC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 504
EcoO109I RGGNCCY 1 cut(s) 370
EcoRII CCWGG 2 cut(s) 373, 385
EcoT14I CCWWGG 2 cut(s) 167, 440
ErhI CCWWGG 2 cut(s) 167, 440
FaeI CATG 2 cut(s) 147, 171
FaiI YATR 5 cut(s) 75, 92, 145, 169, 423
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FaqI GGGAC 2 cut(s) 22, 383
FatI CATG 2 cut(s) 143, 167
FauI CCCGC 1 cut(s) 393
Fnu4HI GCNGC 2 cut(s) 18, 492
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 2 cut(s) 18, 492
GluI GCNGC 2 cut(s) 18, 492
HaeIII GGCC 1 cut(s) 439
HapII CCGG 1 cut(s) 478
Hin1II CATG 2 cut(s) 147, 171
HinfI GANTC 1 cut(s) 452
HpaII CCGG 1 cut(s) 478
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 3 cut(s) 164, 176, 275
Hpy188I TCNGA 1 cut(s) 118
Hpy188III TCNNGA 1 cut(s) 460
Hpy8I GTNNAC 3 cut(s) 164, 176, 275
HpyAV CCTTC 5 cut(s) 50, 62, 121, 121, 211
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 3 cut(s) 143, 264, 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 488, 500
HpyF3I CTNAG 2 cut(s) 84, 472
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 2 cut(s) 147, 171
KflI GGGWCCC 1 cut(s) 370
LpnPI CCDG 9 cut(s) 23, 291, 352, 360, 372, 387, 399, 402, 491
Lsp1109I GCAGC 2 cut(s) 29, 503
LweI GCATC 2 cut(s) 387, 420
MaeII ACGT 1 cut(s) 35
MboII GAAGA 4 cut(s) 35, 245, 350, 497
MhlI GDGCHC 1 cut(s) 287
MlsI TGGCCA 1 cut(s) 439
MluCI AATT 2 cut(s) 27, 69
MluNI TGGCCA 1 cut(s) 439
MnlI CCTC 3 cut(s) 79, 199, 238
Mox20I TGGCCA 1 cut(s) 439
MscI TGGCCA 1 cut(s) 439
MseI TTAA 2 cut(s) 65, 318
Msp20I TGGCCA 1 cut(s) 439
MspI CCGG 1 cut(s) 478
MspR9I CCNGG 3 cut(s) 375, 387, 479
MvaI CCWGG 2 cut(s) 375, 387
MwoI GCNNNNNNNGC 2 cut(s) 488, 500
NciI CCSGG 1 cut(s) 479
NcoI CCATGG 1 cut(s) 167
NlaIII CATG 2 cut(s) 147, 171
NlaIV GGNNCC 2 cut(s) 371, 372
PfeI GAWTC 1 cut(s) 452
PkrI GCNGC 2 cut(s) 19, 493
PpuMI RGGWCCY 1 cut(s) 370
PsiI TTATAA 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 370
Psp6I CCWGG 2 cut(s) 373, 385
PspGI CCWGG 2 cut(s) 373, 385
PspN4I GGNNCC 2 cut(s) 371, 372
PspPI GGNCC 1 cut(s) 370
PspPPI RGGWCCY 1 cut(s) 370
RsaI GTAC 2 cut(s) 165, 268
RsaNI GTAC 2 cut(s) 164, 267
SaqAI TTAA 2 cut(s) 65, 318
SatI GCNGC 2 cut(s) 18, 492
Sau96I GGNCC 1 cut(s) 370
ScrFI CCNGG 3 cut(s) 375, 387, 479
SduI GDGCHC 1 cut(s) 287
SetI ASST 9 cut(s) 22, 38, 85, 90, 113, 126, 249, 324, 379
SfaNI GCATC 2 cut(s) 387, 420
SinI GGWCC 1 cut(s) 370
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 2 cut(s) 27, 69
SsiI CCGC 1 cut(s) 400
StyD4I CCNGG 3 cut(s) 373, 385, 477
StyI CCWWGG 2 cut(s) 167, 440
TaiI ACGT 1 cut(s) 38
TaqI TCGA 2 cut(s) 287, 381
TasI AATT 2 cut(s) 27, 69
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 2 cut(s) 65, 318
Tru9I TTAA 2 cut(s) 65, 318
TscAI CASTG 5 cut(s) 145, 228, 285, 421, 520
TseI GCWGC 2 cut(s) 17, 491
TspDTI ATGAA 3 cut(s) 17, 62, 290
TspRI CASTG 5 cut(s) 145, 228, 285, 421, 520
VpaK11BI GGWCC 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.