RLG00000035366

Protein disulfide isomerase-like 5-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008513
Physical Location & Seq
Reverse (-)
65965080 .. 65967303
2224 bp
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UTR
Exon/CDS
Intron
RLM00000035366

Sequence Viewer

Length: 453 bp
ATGAAAACCAAGTTAGCAGCTTCTCTAATTTTCACGTCCCTTCTTCTTTTCCTGCTCCCAAGTTTAACAATTCATACCAAAGCTGAGGTTATAACCCTAACCGCTGACACCTTCTCCGACAAGGTGAAGGAGAAAGACACTGCATGGTTTGTCAAGTTCTGTGTACCATGGTGTAAACATTGTAAGAATTTGGGGTCATTGTGGGAGGATTTTGGGAAGGCAGTGGAAAGCGAAGATGAAATAGAGGTTGGGGAAGTTGATTGCAGTACGAGTAAACCAGTGTGCTCGAAAGTTGATATTCATTCTTATCCTACATTTAAGCTATTCTTTGATGGAGAAGAAGTTGCCAAATATCAAGGGCCAAGGGATGTTGAATCACTTAAGAATTTTGCCTTGGACCAGGCTGAAGAAGCAGCAAGAAAGGCACATCTTGGCAGTGATACAGAGTTGTGA

Protein Analysis

151

Amino Acids

16.8

Weight (kDa)

5.1

Isoelectric Point (pI)

21.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 30 - 130 8.7e-24 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 92
AciI CCGC 1 cut(s) 102
AcsI RAATTY 2 cut(s) 187, 385
AcuI CTGAAG 1 cut(s) 426
AfaI GTAC 2 cut(s) 165, 268
AflII CTTAAG 1 cut(s) 380
AgsI TTSAA 1 cut(s) 374
AjiI CACGTC 1 cut(s) 36
AjnI CCWGG 1 cut(s) 399
AjuI GAANNNNNNNTTGG 4 cut(s) 231, 263, 377, 409
AluBI AGCT 3 cut(s) 20, 83, 322
AluI AGCT 3 cut(s) 20, 83, 322
Alw21I GWGCWC 1 cut(s) 287
AoxI GGCC 1 cut(s) 359
ApeKI GCWGC 2 cut(s) 17, 413
ApoI RAATTY 2 cut(s) 187, 385
AspS9I GGNCC 2 cut(s) 359, 397
AsuHPI GGTGA 1 cut(s) 136
AvaII GGWCC 1 cut(s) 397
Bbv12I GWGCWC 1 cut(s) 287
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 29, 425
BccI CCATC 1 cut(s) 326
BciT130I CCWGG 1 cut(s) 401
BfrI CTTAAG 1 cut(s) 380
BisI GCNGC 2 cut(s) 18, 414
BlsI GCNGC 2 cut(s) 19, 415
Bme1390I CCNGG 1 cut(s) 401
Bme18I GGWCC 1 cut(s) 397
BmgBI CACGTC 1 cut(s) 36
BmgT120I GGNCC 2 cut(s) 359, 397
BmrFI CCNGG 1 cut(s) 401
Bpu10I CCTNAGC 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 167, 362, 393
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 327, 357
Bse1I ACTGG 1 cut(s) 278
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 3 cut(s) 167, 362, 393
BseGI GGATG 1 cut(s) 373
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 278
BseXI GCAGC 2 cut(s) 29, 425
BshFI GGCC 1 cut(s) 361
BsiHKAI GWGCWC 1 cut(s) 287
BslFI GGGAC 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 287
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 1 cut(s) 102
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 1 cut(s) 76
BspTI CTTAAG 1 cut(s) 380
BsrI ACTGG 1 cut(s) 278
BssECI CCNNGG 3 cut(s) 167, 362, 393
BssT1I CCWWGG 3 cut(s) 167, 362, 393
Bst2UI CCWGG 1 cut(s) 401
BstAFI CTTAAG 1 cut(s) 380
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 373
BstMWI GCNNNNNNNGC 2 cut(s) 410, 422
BstNI CCWGG 1 cut(s) 401
BstSCI CCNGG 1 cut(s) 399
BstV1I GCAGC 2 cut(s) 29, 425
BsuRI GGCC 1 cut(s) 361
BtgI CCRYGG 1 cut(s) 167
BtrI CACGTC 1 cut(s) 36
BtsCI GGATG 1 cut(s) 373
BtsI GCAGTG 3 cut(s) 138, 228, 442
BtsIMutI CAGTG 4 cut(s) 138, 228, 285, 442
Cfr13I GGNCC 2 cut(s) 359, 397
Csp6I GTAC 2 cut(s) 164, 267
CviAII CATG 2 cut(s) 144, 168
CviJI RGCY 5 cut(s) 20, 83, 322, 361, 404
CviKI_1 RGCY 5 cut(s) 20, 83, 322, 361, 404
CviQI GTAC 2 cut(s) 164, 267
DdeI CTNAG 1 cut(s) 84
Eco130I CCWWGG 3 cut(s) 167, 362, 393
Eco47I GGWCC 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 399
EcoT14I CCWWGG 3 cut(s) 167, 362, 393
ErhI CCWWGG 3 cut(s) 167, 362, 393
FaeI CATG 2 cut(s) 147, 171
FaiI YATR 4 cut(s) 75, 92, 145, 169
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FaqI GGGAC 1 cut(s) 22
FatI CATG 2 cut(s) 143, 167
Fnu4HI GCNGC 2 cut(s) 18, 414
FokI GGATG 1 cut(s) 380
Fsp4HI GCNGC 2 cut(s) 18, 414
GluI GCNGC 2 cut(s) 18, 414
HaeIII GGCC 1 cut(s) 361
Hin1II CATG 2 cut(s) 147, 171
HinfI GANTC 1 cut(s) 374
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 3 cut(s) 164, 176, 275
Hpy188I TCNGA 1 cut(s) 118
Hpy8I GTNNAC 3 cut(s) 164, 176, 275
HpyAV CCTTC 4 cut(s) 50, 121, 121, 211
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 2 cut(s) 143, 264
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 422
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 2 cut(s) 147, 171
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 4 cut(s) 65, 291, 386, 413
Lsp1109I GCAGC 2 cut(s) 29, 425
MaeII ACGT 1 cut(s) 35
MboII GAAGA 4 cut(s) 35, 245, 350, 419
MhlI GDGCHC 1 cut(s) 287
MluCI AATT 4 cut(s) 27, 69, 187, 385
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 3 cut(s) 79, 199, 238
MseI TTAA 3 cut(s) 65, 318, 381
MspA1I CMGCKG 1 cut(s) 104
MspCI CTTAAG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 401
MvaI CCWGG 1 cut(s) 401
MwoI GCNNNNNNNGC 2 cut(s) 410, 422
NcoI CCATGG 1 cut(s) 167
NlaIII CATG 2 cut(s) 147, 171
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 2 cut(s) 19, 415
PsiI TTATAA 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 399
PspGI CCWGG 1 cut(s) 399
PspPI GGNCC 2 cut(s) 359, 397
RsaI GTAC 2 cut(s) 165, 268
RsaNI GTAC 2 cut(s) 164, 267
SaqAI TTAA 3 cut(s) 65, 318, 381
SatI GCNGC 2 cut(s) 18, 414
Sau96I GGNCC 2 cut(s) 359, 397
ScrFI CCNGG 1 cut(s) 401
SduI GDGCHC 1 cut(s) 287
SetI ASST 8 cut(s) 22, 38, 85, 90, 113, 126, 249, 324
SinI GGWCC 1 cut(s) 397
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 4 cut(s) 27, 69, 187, 385
SsiI CCGC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 399
StyI CCWWGG 3 cut(s) 167, 362, 393
TaiI ACGT 1 cut(s) 38
TaqI TCGA 1 cut(s) 287
TasI AATT 4 cut(s) 27, 69, 187, 385
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 3 cut(s) 65, 318, 381
Tru9I TTAA 3 cut(s) 65, 318, 381
TscAI CASTG 4 cut(s) 145, 228, 285, 442
TseI GCWGC 2 cut(s) 17, 413
TspDTI ATGAA 4 cut(s) 17, 62, 252, 290
TspRI CASTG 4 cut(s) 145, 228, 285, 442
Vha464I CTTAAG 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 397
XapI RAATTY 2 cut(s) 187, 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.