MD11G1130500.v1.1

Protein disulfide isomerase-like 5-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008513
Physical Location & Seq
Forward (+)
11840449 .. 11843190
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1130500.v1.1.491

Sequence Viewer

Length: 450 bp
ATGAAAACCCACTCTCCTTGTCTAGTTTTCATCTCAGTTCTTCTCTTTCTTCTTCCAATTCTAACAACATATACCGAAGCCGAGGTTATAACCCTAACCGCTGACACCTTCTCCGACAAGGTGAAGGAGAAAGACACGGCATGGTTTGTGAAATTTTGTGTGCCATGGTGTAAGCATTGTAAGAACTTGGGTACATTGTGGGAGGACCTTGGGAAGACAATGGAAGGTGAAGACGAAATAGAGGTTGGGGAGGTTGATTGTAGTACGAGTAAACCAGTATGTTCAAAAGTTGACATTCATTCGTATCCTACGTTTAAAGTATTCTATGATGGTGAAGAAGTTGCAAAATATCAAGGGCCGAGGGATGTTGACTCACTAAAGAAGTTTGTCTTGGAAGAAGCTGAAAAGGCAGCAACAAAGGCACAGCTTGACAGTGATAAGGAGTTGTAG

Protein Analysis

150

Amino Acids

16.85

Weight (kDa)

4.83

Isoelectric Point (pI)

26.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 29 - 130 1e-24 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 89
AciI CCGC 1 cut(s) 99
AcsI RAATTY 1 cut(s) 152
AfaI GTAC 2 cut(s) 193, 265
AgsI TTSAA 1 cut(s) 285
AjuI GAANNNNNNNTTGG 4 cut(s) 228, 260, 374, 406
AluBI AGCT 2 cut(s) 401, 427
AluI AGCT 2 cut(s) 401, 427
AoxI GGCC 1 cut(s) 356
ApeKI GCWGC 1 cut(s) 410
ApoI RAATTY 1 cut(s) 152
AspS9I GGNCC 2 cut(s) 205, 356
AsuHPI GGTGA 3 cut(s) 133, 239, 344
AvaII GGWCC 1 cut(s) 205
BbsI GAAGAC 2 cut(s) 221, 237
BbvI GCAGC 1 cut(s) 422
BccI CCATC 1 cut(s) 323
BceAI ACGGC 1 cut(s) 153
BciVI GTATCC 1 cut(s) 315
BfaI CTAG 1 cut(s) 23
BfuI GTATCC 1 cut(s) 315
BisI GCNGC 1 cut(s) 411
BlsI GCNGC 1 cut(s) 412
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 2 cut(s) 205, 356
BpiI GAAGAC 2 cut(s) 221, 237
BsaJI CCNNGG 4 cut(s) 81, 164, 208, 359
BsaXI ACNNNNNCTCC 3 cut(s) 28, 95, 125
Bse1I ACTGG 1 cut(s) 275
BseDI CCNNGG 4 cut(s) 81, 164, 208, 359
BseGI GGATG 1 cut(s) 370
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 275
BseXI GCAGC 1 cut(s) 422
BshFI GGCC 1 cut(s) 358
BsnI GGCC 1 cut(s) 358
Bsp19I CCATGG 1 cut(s) 164
BspACI CCGC 1 cut(s) 99
BspANI GGCC 1 cut(s) 358
BspCNI CTCAG 1 cut(s) 47
BsrI ACTGG 1 cut(s) 275
BssECI CCNNGG 4 cut(s) 81, 164, 208, 359
BssT1I CCWWGG 2 cut(s) 164, 208
Bst4CI ACNGT 1 cut(s) 434
BstDEI CTNAG 1 cut(s) 34
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 1 cut(s) 370
BstMWI GCNNNNNNNGC 2 cut(s) 407, 419
BstV1I GCAGC 1 cut(s) 422
BstV2I GAAGAC 2 cut(s) 221, 237
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 1 cut(s) 358
BtgI CCRYGG 1 cut(s) 164
BtsCI GGATG 1 cut(s) 370
BtsIMutI CAGTG 1 cut(s) 439
Cfr13I GGNCC 2 cut(s) 205, 356
Csp6I GTAC 2 cut(s) 192, 264
CspCI CAANNNNNGTGG 1 cut(s) 34
CviAII CATG 2 cut(s) 141, 165
CviJI RGCY 4 cut(s) 80, 358, 401, 427
CviKI_1 RGCY 4 cut(s) 80, 358, 401, 427
CviQI GTAC 2 cut(s) 192, 264
DdeI CTNAG 1 cut(s) 34
DraI TTTAAA 1 cut(s) 316
Eco130I CCWWGG 2 cut(s) 164, 208
Eco47I GGWCC 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 164, 208
ErhI CCWWGG 2 cut(s) 164, 208
FaeI CATG 2 cut(s) 144, 168
FaiI YATR 7 cut(s) 70, 72, 89, 142, 166, 280, 327
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FatI CATG 2 cut(s) 140, 164
Fnu4HI GCNGC 1 cut(s) 411
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 1 cut(s) 411
FspBI CTAG 1 cut(s) 23
GluI GCNGC 1 cut(s) 411
HaeIII GGCC 1 cut(s) 358
Hin1II CATG 2 cut(s) 144, 168
HincII GTYRAC 2 cut(s) 292, 370
HindII GTYRAC 2 cut(s) 292, 370
HinfI GANTC 1 cut(s) 371
HphI GGTGA 3 cut(s) 133, 239, 344
Hpy166II GTNNAC 3 cut(s) 272, 292, 370
Hpy188I TCNGA 1 cut(s) 115
Hpy8I GTNNAC 3 cut(s) 272, 292, 370
HpyAV CCTTC 3 cut(s) 118, 118, 218
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 1 cut(s) 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 407, 419
HpyF3I CTNAG 1 cut(s) 34
HpySE526I ACGT 1 cut(s) 311
Hsp92II CATG 2 cut(s) 144, 168
LpnPI CCDG 1 cut(s) 288
Lsp1109I GCAGC 1 cut(s) 422
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 311
MboII GAAGA 7 cut(s) 32, 41, 44, 226, 242, 347, 407
MluCI AATT 2 cut(s) 57, 152
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 5 cut(s) 76, 196, 235, 244, 354
MseI TTAA 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 101
MwoI GCNNNNNNNGC 2 cut(s) 407, 419
NcoI CCATGG 1 cut(s) 164
NlaIII CATG 2 cut(s) 144, 168
NmeAIII GCCGAG 2 cut(s) 106, 384
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PkrI GCNGC 1 cut(s) 412
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
PpuMI RGGWCCY 1 cut(s) 205
PsiI TTATAA 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 205
PspPI GGNCC 2 cut(s) 205, 356
PspPPI RGGWCCY 1 cut(s) 205
RsaI GTAC 2 cut(s) 193, 265
RsaNI GTAC 2 cut(s) 192, 264
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 1 cut(s) 411
Sau96I GGNCC 2 cut(s) 205, 356
SchI GAGTC 1 cut(s) 365
SinI GGWCC 1 cut(s) 205
Sse9I AATT 2 cut(s) 57, 152
SsiI CCGC 1 cut(s) 99
SspMI CTAG 1 cut(s) 23
StyI CCWWGG 2 cut(s) 164, 208
TaaI ACNGT 1 cut(s) 434
TaiI ACGT 1 cut(s) 314
TasI AATT 2 cut(s) 57, 152
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 1 cut(s) 439
TseI GCWGC 1 cut(s) 410
TspDTI ATGAA 3 cut(s) 17, 19, 287
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 205
XapI RAATTY 1 cut(s) 152
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.