FvH4_3g34301

Glycosyl hydrolases family 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009671
Physical Location & Seq
Reverse (-)
29811327 .. 29812648
1322 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34301.t1

Sequence Viewer

Length: 954 bp
ATGAACTCAACCAGGCTACAGCTGTTTCTTTTCCTTCTAATGACATTGTTCTTCACGACACTGTCAACAGCCCAAGCAGCCCCAGCATACTCAAAACTCTTCAGGGAATACATAGGAGCTGAGGACAACGGTGTCAGATTCACAGATGTACCAATAGATTCCCGAGTCGAGTTTCACTTCATCCTCTCCTTTGCAATTGACTACACAACATCTTCCCATCCTAACTGCACCAATAACTTCACAACATCTTCCCATCCAAAACCCACCAATGGCAACTTTAACGTGTTCTGGGACACAAAGAATCTCAGCCCCTCTCATGTTTCCGCTATTAAGGCTAGTCATCCAAATGCTAAAGTTGCTCTGAGTCTTGGAGGAGATACTGTAAAAGGTAACCAGTATGCATACTTCAGTCCTACATCAATCAATTCTTGGGTCAGAAATGCTATTGCTTCAATAGTTCAGATAACGAAAGAATACGAGTTGGACGGAATTGATATCGACTGTGAACACTTCCAGACAGATCCCAATACATTTGCTGAATGCATTGGGCGACTATTATTCCACCTTAAACAAAATAATGTTGTGTCCTTCACATCAATTGCACCATATGAAGATGATTCCGTGCAACCATATTACTTGGCATTGTGGAGGAAGTATGGGCACCTCATTGACTATGTTAACTTTCAGTTTTATGCCTATAGTAAGGGCACAACAGTAACTCAGTTTATGAAATATTTTGAGACCCAGAGCTCAAATTACAAAGGTGGCAAGATTCTAGTGAGCATTGGAACAGACAATAGTGGGGGTTTGTCTCCTAAAAATGGGTTCTTTGAGGCTTGTGCTAGGCTTAAAAGTCAGGGAAAGCTTCATGGCATTTTTATTTGGTCCGCAGACGACTCCAAGAAGGCTTCTTTTCATCATGAGAATCAATCACAAAGATTTCTTGCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

318

Amino Acids

35.68

Weight (kDa)

7.17

Isoelectric Point (pI)

23.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 98 - 234 7.1e-13 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 660
AciI CCGC 2 cut(s) 324, 888
AclWI GGATC 1 cut(s) 515
AcuI CTGAAG 2 cut(s) 85, 391
AfaI GTAC 1 cut(s) 150
AfiI CCNNNNNNNGG 2 cut(s) 269, 821
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 1 cut(s) 453
AjnI CCWGG 1 cut(s) 11
AluBI AGCT 5 cut(s) 22, 119, 750, 865, 951
AluI AGCT 5 cut(s) 22, 119, 750, 865, 951
Alw21I GWGCWC 1 cut(s) 752
Alw26I GTCTC 2 cut(s) 734, 816
AlwI GGATC 1 cut(s) 515
Ama87I CYCGRG 1 cut(s) 162
ApeKI GCWGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 885
AsuNHI GCTAGC 1 cut(s) 947
AvaI CYCGRG 1 cut(s) 162
AvaII GGWCC 1 cut(s) 885
BaeGI GKGCMC 2 cut(s) 663, 710
BaeI ACNNNNGTAYC 2 cut(s) 132, 165
BanI GGYRCC 1 cut(s) 660
BanII GRGCYC 1 cut(s) 752
BarI GAAGNNNNNNTAC 2 cut(s) 389, 421
Bbv12I GWGCWC 1 cut(s) 752
BbvCI CCTCAGC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 89
BccI CCATC 2 cut(s) 225, 261
BciT130I CCWGG 1 cut(s) 13
BcoDI GTCTC 2 cut(s) 734, 816
BfaI CTAG 4 cut(s) 336, 776, 843, 948
BfmI CTRYAG 2 cut(s) 17, 697
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 13
Bme18I GGWCC 1 cut(s) 885
BmeT110I CYCGRG 1 cut(s) 162
BmgT120I GGNCC 1 cut(s) 885
BmiI GGNNCC 1 cut(s) 662
BmrFI CCNGG 1 cut(s) 13
BmtI GCTAGC 1 cut(s) 951
Bpu10I CCTNAGC 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 734
BsaXI ACNNNNNCTCC 2 cut(s) 366, 396
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 821
Bse1I ACTGG 1 cut(s) 394
BseBI CCWGG 1 cut(s) 13
BseGI GGATG 4 cut(s) 180, 217, 253, 340
BseLI CCNNNNNNNGG 2 cut(s) 269, 821
BseMII CTCAG 4 cut(s) 111, 319, 353, 734
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 387
BseSI GKGCMC 2 cut(s) 663, 710
BseXI GCAGC 1 cut(s) 89
BseYI CCCAGC 1 cut(s) 82
BsgI GTGCAG 1 cut(s) 211
BshNI GGYRCC 1 cut(s) 660
BsiHKAI GWGCWC 1 cut(s) 752
BsiHKCI CYCGRG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 2 cut(s) 269, 821
BsmAI GTCTC 2 cut(s) 734, 816
BsmFI GGGAC 1 cut(s) 305
BsmI GAATGC 1 cut(s) 545
Bso31I GGTCTC 1 cut(s) 734
BsoBI CYCGRG 1 cut(s) 162
Bsp1286I GDGCHC 3 cut(s) 663, 710, 752
Bsp143I GATC 1 cut(s) 520
BspACI CCGC 2 cut(s) 324, 888
BspCNI CTCAG 4 cut(s) 112, 318, 354, 733
BspHI TCATGA 1 cut(s) 919
BspLI GGNNCC 1 cut(s) 662
BspOI GCTAGC 1 cut(s) 951
BspPI GGATC 1 cut(s) 515
BspT107I GGYRCC 1 cut(s) 660
BspTNI GGTCTC 1 cut(s) 734
BsrI ACTGG 1 cut(s) 394
BssMI GATC 1 cut(s) 520
Bst2UI CCWGG 1 cut(s) 13
Bst4CI ACNGT 5 cut(s) 63, 131, 382, 503, 715
Bst6I CTCTTC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 949
BstDEI CTNAG 4 cut(s) 120, 305, 362, 720
BstEII GGTNACC 1 cut(s) 389
BstF5I GGATG 4 cut(s) 180, 217, 253, 340
BstKTI GATC 1 cut(s) 523
BstMAI GTCTC 2 cut(s) 734, 816
BstMBI GATC 1 cut(s) 520
BstMWI GCNNNNNNNGC 4 cut(s) 77, 83, 332, 356
BstNI CCWGG 1 cut(s) 13
BstPI GGTNACC 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 11
BstSFI CTRYAG 2 cut(s) 17, 697
BstSLI GKGCMC 2 cut(s) 663, 710
BstV1I GCAGC 1 cut(s) 89
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BtsCI GGATG 4 cut(s) 180, 217, 253, 340
BtsIMutI CAGTG 1 cut(s) 59
Cac8I GCNNGC 1 cut(s) 949
CciI TCATGA 1 cut(s) 919
Cfr13I GGNCC 1 cut(s) 885
Csp6I GTAC 1 cut(s) 149
CviAII CATG 3 cut(s) 317, 869, 920
CviQI GTAC 1 cut(s) 149
DdeI CTNAG 4 cut(s) 120, 305, 362, 720
DpnI GATC 1 cut(s) 522
DpnII GATC 1 cut(s) 520
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 750
Eco24I GRGCYC 1 cut(s) 752
Eco31I GGTCTC 1 cut(s) 734
Eco32I GATATC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 885
Eco53kI GAGCTC 1 cut(s) 750
Eco57I CTGAAG 2 cut(s) 85, 391
Eco88I CYCGRG 1 cut(s) 162
Eco91I GGTNACC 1 cut(s) 389
EcoICRI GAGCTC 1 cut(s) 750
EcoO65I GGTNACC 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 11
EcoRV GATATC 1 cut(s) 496
EcoT22I ATGCAT 2 cut(s) 403, 545
EcoT38I GRGCYC 1 cut(s) 752
FaeI CATG 3 cut(s) 320, 872, 923
FaqI GGGAC 1 cut(s) 305
FatI CATG 3 cut(s) 316, 868, 919
FauNDI CATATG 1 cut(s) 607
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 4 cut(s) 167, 204, 240, 327
FriOI GRGCYC 1 cut(s) 752
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 4 cut(s) 336, 776, 843, 948
GluI GCNGC 1 cut(s) 78
GsaI CCCAGC 1 cut(s) 86
Hin1II CATG 3 cut(s) 320, 872, 923
HincII GTYRAC 2 cut(s) 66, 679
HindII GTYRAC 2 cut(s) 66, 679
HindIII AAGCTT 1 cut(s) 863
HinfI GANTC 9 cut(s) 138, 158, 165, 301, 364, 617, 772, 896, 925
HpaI GTTAAC 1 cut(s) 679
Hpy166II GTNNAC 3 cut(s) 66, 506, 679
Hpy188I TCNGA 4 cut(s) 137, 363, 437, 462
Hpy188III TCNNGA 4 cut(s) 55, 162, 514, 920
Hpy8I GTNNAC 3 cut(s) 66, 506, 679
HpyAV CCTTC 3 cut(s) 44, 598, 898
HpyCH4III ACNGT 5 cut(s) 63, 131, 382, 503, 715
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 6 cut(s) 194, 228, 401, 543, 602, 625
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 83, 332, 356
HpyF3I CTNAG 4 cut(s) 120, 305, 362, 720
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 3 cut(s) 320, 872, 923
KspAI GTTAAC 1 cut(s) 679
Kzo9I GATC 1 cut(s) 520
LmnI GCTCC 1 cut(s) 116
LpnPI CCDG 8 cut(s) 25, 88, 96, 274, 407, 527, 758, 842
Lsp1109I GCAGC 1 cut(s) 89
MaeI CTAG 4 cut(s) 336, 776, 843, 948
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 2 cut(s) 389, 715
MalI GATC 1 cut(s) 522
MboI GATC 1 cut(s) 520
MboII GAAGA 5 cut(s) 43, 91, 204, 240, 623
MfeI CAATTG 2 cut(s) 195, 597
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 3 cut(s) 663, 710, 752
MluCI AATT 5 cut(s) 195, 424, 489, 597, 754
MlyI GAGTC 3 cut(s) 174, 373, 890
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 7 cut(s) 115, 194, 322, 365, 642, 674, 826
Mph1103I ATGCAT 2 cut(s) 403, 545
MseI TTAA 5 cut(s) 279, 330, 567, 678, 849
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 22
MspR9I CCNGG 1 cut(s) 13
MunI CAATTG 2 cut(s) 195, 597
Mva1269I GAATGC 1 cut(s) 545
MvaI CCWGG 1 cut(s) 13
MwoI GCNNNNNNNGC 4 cut(s) 77, 83, 332, 356
NdeI CATATG 1 cut(s) 607
NdeII GATC 1 cut(s) 520
NheI GCTAGC 1 cut(s) 947
NlaIII CATG 3 cut(s) 320, 872, 923
NlaIV GGNNCC 1 cut(s) 662
NsiI ATGCAT 2 cut(s) 403, 545
PagI TCATGA 1 cut(s) 919
PctI GAATGC 1 cut(s) 545
PfeI GAWTC 6 cut(s) 138, 158, 301, 617, 772, 925
PflFI GACNNNGTC 1 cut(s) 61
PkrI GCNGC 1 cut(s) 79
PleI GAGTC 3 cut(s) 173, 372, 890
PpsI GAGTC 3 cut(s) 173, 372, 890
Psp124BI GAGCTC 1 cut(s) 752
Psp6I CCWGG 1 cut(s) 11
PspEI GGTNACC 1 cut(s) 389
PspFI CCCAGC 1 cut(s) 82
PspGI CCWGG 1 cut(s) 11
PspN4I GGNNCC 1 cut(s) 662
PspPI GGNCC 1 cut(s) 885
PsuI RGATCY 1 cut(s) 520
PsyI GACNNNGTC 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 22
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 1 cut(s) 752
SaqAI TTAA 5 cut(s) 279, 330, 567, 678, 849
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 1 cut(s) 520
Sau96I GGNCC 1 cut(s) 885
SchI GAGTC 3 cut(s) 174, 373, 890
ScrFI CCNGG 1 cut(s) 13
SduI GDGCHC 3 cut(s) 663, 710, 752
SfcI CTRYAG 2 cut(s) 17, 697
SinI GGWCC 1 cut(s) 885
SmiMI CAYNNNNRTG 1 cut(s) 345
Sse9I AATT 5 cut(s) 195, 424, 489, 597, 754
SsiI CCGC 2 cut(s) 324, 888
SspI AATATT 1 cut(s) 734
SspMI CTAG 4 cut(s) 336, 776, 843, 948
SstI GAGCTC 1 cut(s) 752
StyD4I CCNGG 1 cut(s) 11
TaaI ACNGT 5 cut(s) 63, 131, 382, 503, 715
TaiI ACGT 1 cut(s) 285
TaqI TCGA 2 cut(s) 168, 498
TasI AATT 5 cut(s) 195, 424, 489, 597, 754
TfiI GAWTC 6 cut(s) 138, 158, 301, 617, 772, 925
Tru1I TTAA 5 cut(s) 279, 330, 567, 678, 849
Tru9I TTAA 5 cut(s) 279, 330, 567, 678, 849
TscAI CASTG 1 cut(s) 66
TseI GCWGC 1 cut(s) 77
TspDTI ATGAA 6 cut(s) 17, 169, 624, 743, 857, 905
TspGWI ACGGA 2 cut(s) 501, 610
TspRI CASTG 1 cut(s) 66
Tth111I GACNNNGTC 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 885
XspI CTAG 4 cut(s) 336, 776, 843, 948
Zsp2I ATGCAT 2 cut(s) 403, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.