MD11G1119200.v1.1

Glycosyl hydrolases family 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009671
Physical Location & Seq
Forward (+)
10872402 .. 10873341
940 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1119200.v1.1.491

Sequence Viewer

Length: 918 bp
ATGACTTCGTTTAAGCTCCTACCGTTCCTTTTTCTTCAAATGTTTTTCACAGCAGCGTCAATGATCAAAGCAGCCCCAGCAAACCCAAAGCTCTTTAGAGAATACATAGGAGCTGAGAACAAAGGTGTCACTTTCACAGAAGTACCCGTTGATTCCAATGTTGAGTTTCACTTCATCCTCTCCTTTGCTATTGATTATACAACACAATCTCATAATCCTTCTCCCACCAACGGAGACTTTAATGTCTTCTGGGACACAGAGAACCTCACCGCTGCCCATGTTTCCGCCATTAAGGCCCTTCATCCAAATGCTAAAGTAGCTCTGAGTCTTGGAGGTGATACCGTTGGCAACAAAAATGTGTCCTTCAATCCTAAATCAATCAATTCTTGGGTTAGAAATGCTGTCTACTCACTAACTCAGATAATCAGAAAATATCATTTGGATGGAATTGATATTGACTACGAACACTTCCAGACAGATCCCAATACATTTGCCGAATGCATTGGGCGACTGTTGTTCTCCCTTAAACAAAACAATATTGTGTCCTTCACATCAATAGCACCATATGATGATGACTCGGTGCAGCCAAATTACTTGGCATTGTGGGGGAAGTACGGGCATCTCATTGACTATGTTAACTTTCAGTTCTATGCCTACGACAAGGGCACGACTATAACTCAGTTTTTGAAATATTTTGAGATGCAGAGCTCAAATTACAAGGGTGGCAAGATTCTAGTGAGCTTTGGAACAGATAACAGCGGGGGTTTGTCTCCTGAACACGGGTTCTTTGCGGCTTGTACTAGGCTTAAACGCGAGGGCAAACTTCATGGCATTTTCGTTTGGTCTGCAGATGACTCCAGGAAGGATCATTTTCGTTACGAGAAGCAATCACAAGCCTTTCTATCTAGTGTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

306

Amino Acids

34.43

Weight (kDa)

6.55

Isoelectric Point (pI)

30.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 77 - 219 2.3e-11 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
AccI GTMKAC 1 cut(s) 405
AccII CGCG 1 cut(s) 813
AciI CCGC 4 cut(s) 270, 285, 759, 791
AclWI GGATC 2 cut(s) 473, 873
AfaI GTAC 3 cut(s) 144, 614, 799
AfiI CCNNNNNNNGG 2 cut(s) 230, 779
AgsI TTSAA 3 cut(s) 38, 367, 688
AjnI CCWGG 1 cut(s) 857
AluBI AGCT 6 cut(s) 16, 91, 113, 320, 708, 741
AluI AGCT 6 cut(s) 16, 91, 113, 320, 708, 741
Alw21I GWGCWC 1 cut(s) 710
Alw26I GTCTC 2 cut(s) 228, 774
AlwI GGATC 2 cut(s) 473, 873
AoxI GGCC 1 cut(s) 294
ApeKI GCWGC 4 cut(s) 53, 71, 272, 583
AspS9I GGNCC 1 cut(s) 295
AsuHPI GGTGA 2 cut(s) 259, 347
BaeGI GKGCMC 1 cut(s) 668
BaeI ACNNNNGTAYC 2 cut(s) 126, 159
BanII GRGCYC 1 cut(s) 710
BbsI GAAGAC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 710
BbvI GCAGC 4 cut(s) 65, 83, 259, 595
BccI CCATC 1 cut(s) 437
BciT130I CCWGG 1 cut(s) 859
BclI TGATCA 1 cut(s) 63
BcoDI GTCTC 2 cut(s) 228, 774
BfaI CTAG 3 cut(s) 734, 801, 906
BfmI CTRYAG 1 cut(s) 846
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 5 cut(s) 54, 72, 273, 584, 792
BlsI GCNGC 5 cut(s) 55, 73, 274, 585, 793
Bme1390I CCNGG 1 cut(s) 859
BmgT120I GGNCC 1 cut(s) 295
BmrFI CCNGG 1 cut(s) 859
BmsI GCATC 2 cut(s) 628, 690
BpiI GAAGAC 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 841
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 779
BseBI CCWGG 1 cut(s) 859
BseGI GGATG 3 cut(s) 174, 301, 448
BseLI CCNNNNNNNGG 2 cut(s) 230, 779
BseMII CTCAG 4 cut(s) 105, 314, 431, 692
BseSI GKGCMC 1 cut(s) 668
BseXI GCAGC 4 cut(s) 65, 83, 259, 595
BseYI CCCAGC 1 cut(s) 76
BsgI GTGCAG 1 cut(s) 602
Bsh1236I CGCG 1 cut(s) 813
BshFI GGCC 1 cut(s) 296
BsiHKAI GWGCWC 1 cut(s) 710
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 230, 779
BsmAI GTCTC 2 cut(s) 228, 774
BsmFI GGGAC 1 cut(s) 266
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 668, 710
Bsp143I GATC 3 cut(s) 63, 478, 865
BspACI CCGC 4 cut(s) 270, 285, 759, 791
BspANI GGCC 1 cut(s) 296
BspCNI CTCAG 4 cut(s) 106, 315, 430, 691
BspFNI CGCG 1 cut(s) 813
BspMAI CTGCAG 1 cut(s) 850
BspPI GGATC 2 cut(s) 473, 873
BssMI GATC 3 cut(s) 63, 478, 865
Bst2UI CCWGG 1 cut(s) 859
Bst4CI ACNGT 3 cut(s) 24, 343, 513
BstDEI CTNAG 4 cut(s) 114, 323, 417, 678
BstF5I GGATG 3 cut(s) 174, 301, 448
BstFNI CGCG 1 cut(s) 813
BstKTI GATC 3 cut(s) 66, 481, 868
BstMAI GTCTC 2 cut(s) 228, 774
BstMBI GATC 3 cut(s) 63, 478, 865
BstMWI GCNNNNNNNGC 3 cut(s) 77, 293, 317
BstNI CCWGG 1 cut(s) 859
BstSCI CCNGG 1 cut(s) 857
BstSFI CTRYAG 1 cut(s) 846
BstSLI GKGCMC 1 cut(s) 668
BstUI CGCG 1 cut(s) 813
BstV1I GCAGC 4 cut(s) 65, 83, 259, 595
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 1 cut(s) 296
BtsCI GGATG 3 cut(s) 174, 301, 448
Cfr13I GGNCC 1 cut(s) 295
CseI GACGC 1 cut(s) 45
Csp6I GTAC 3 cut(s) 143, 613, 798
CviAII CATG 2 cut(s) 278, 827
CviQI GTAC 3 cut(s) 143, 613, 798
DdeI CTNAG 4 cut(s) 114, 323, 417, 678
DpnI GATC 3 cut(s) 65, 480, 867
DpnII GATC 3 cut(s) 63, 478, 865
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EciI GGCGGA 1 cut(s) 274
Ecl136II GAGCTC 1 cut(s) 708
Eco24I GRGCYC 1 cut(s) 710
Eco53kI GAGCTC 1 cut(s) 708
EcoICRI GAGCTC 1 cut(s) 708
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 857
EcoT22I ATGCAT 1 cut(s) 503
EcoT38I GRGCYC 1 cut(s) 710
FaeI CATG 2 cut(s) 281, 830
FaqI GGGAC 1 cut(s) 266
FatI CATG 2 cut(s) 277, 826
FauI CCCGC 1 cut(s) 752
FauNDI CATATG 1 cut(s) 565
FbaI TGATCA 1 cut(s) 63
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 5 cut(s) 54, 72, 273, 584, 792
FokI GGATG 3 cut(s) 161, 288, 455
FriOI GRGCYC 1 cut(s) 710
Fsp4HI GCNGC 5 cut(s) 54, 72, 273, 584, 792
FspBI CTAG 3 cut(s) 734, 801, 906
GluI GCNGC 5 cut(s) 54, 72, 273, 584, 792
GsaI CCCAGC 1 cut(s) 80
GsuI CTGGAG 1 cut(s) 841
HaeIII GGCC 1 cut(s) 296
HgaI GACGC 1 cut(s) 45
Hin1II CATG 2 cut(s) 281, 830
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HinfI GANTC 5 cut(s) 152, 325, 575, 730, 854
HpaI GTTAAC 1 cut(s) 637
HphI GGTGA 2 cut(s) 259, 347
Hpy166II GTNNAC 2 cut(s) 406, 637
Hpy188I TCNGA 3 cut(s) 324, 420, 428
Hpy188III TCNNGA 2 cut(s) 472, 773
Hpy8I GTNNAC 2 cut(s) 406, 637
HpyAV CCTTC 5 cut(s) 228, 308, 373, 556, 856
HpyCH4III ACNGT 3 cut(s) 24, 343, 513
HpyCH4V TGCA 4 cut(s) 501, 583, 703, 848
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 293, 317
HpyF3I CTNAG 4 cut(s) 114, 323, 417, 678
Hsp92II CATG 2 cut(s) 281, 830
Ksp22I TGATCA 1 cut(s) 63
KspAI GTTAAC 1 cut(s) 637
Kzo9I GATC 3 cut(s) 63, 478, 865
LmnI GCTCC 2 cut(s) 21, 110
LpnPI CCDG 6 cut(s) 90, 235, 485, 786, 844, 871
Lsp1109I GCAGC 4 cut(s) 65, 83, 259, 595
LweI GCATC 2 cut(s) 628, 690
MaeI CTAG 3 cut(s) 734, 801, 906
MaeIII GTNAC 2 cut(s) 127, 875
MalI GATC 3 cut(s) 65, 480, 867
MboI GATC 3 cut(s) 63, 478, 865
MboII GAAGA 2 cut(s) 26, 238
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 2 cut(s) 668, 710
MluCI AATT 5 cut(s) 382, 447, 589, 712, 912
MlyI GAGTC 3 cut(s) 334, 569, 848
MnlI CCTC 4 cut(s) 188, 275, 326, 808
Mph1103I ATGCAT 1 cut(s) 503
MseI TTAA 6 cut(s) 12, 240, 291, 525, 636, 807
MslI CAYNNNNRTG 2 cut(s) 306, 441
MspA1I CMGCKG 2 cut(s) 272, 759
MspR9I CCNGG 1 cut(s) 859
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 1 cut(s) 859
MvnI CGCG 1 cut(s) 813
MwoI GCNNNNNNNGC 3 cut(s) 77, 293, 317
NdeI CATATG 1 cut(s) 565
NdeII GATC 3 cut(s) 63, 478, 865
NlaIII CATG 2 cut(s) 281, 830
NmuCI GTSAC 1 cut(s) 127
NsiI ATGCAT 1 cut(s) 503
PctI GAATGC 1 cut(s) 503
PfeI GAWTC 2 cut(s) 152, 730
PfoI TCCNGGA 1 cut(s) 857
PkrI GCNGC 5 cut(s) 55, 73, 274, 585, 793
PleI GAGTC 3 cut(s) 333, 569, 848
PpsI GAGTC 3 cut(s) 333, 569, 848
Psp124BI GAGCTC 1 cut(s) 710
Psp6I CCWGG 1 cut(s) 857
PspFI CCCAGC 1 cut(s) 76
PspGI CCWGG 1 cut(s) 857
PspPI GGNCC 1 cut(s) 295
PstI CTGCAG 1 cut(s) 850
PsuI RGATCY 1 cut(s) 478
RsaI GTAC 3 cut(s) 144, 614, 799
RsaNI GTAC 3 cut(s) 143, 613, 798
RseI CAYNNNNRTG 2 cut(s) 306, 441
SacI GAGCTC 1 cut(s) 710
SaqAI TTAA 6 cut(s) 12, 240, 291, 525, 636, 807
SatI GCNGC 5 cut(s) 54, 72, 273, 584, 792
Sau3AI GATC 3 cut(s) 63, 478, 865
Sau96I GGNCC 1 cut(s) 295
SchI GAGTC 3 cut(s) 334, 569, 848
ScrFI CCNGG 1 cut(s) 859
SduI GDGCHC 2 cut(s) 668, 710
SetI ASST 9 cut(s) 18, 93, 115, 127, 267, 322, 337, 710, 743
SfaNI GCATC 2 cut(s) 628, 690
SfcI CTRYAG 1 cut(s) 846
SmiMI CAYNNNNRTG 2 cut(s) 306, 441
Sse9I AATT 5 cut(s) 382, 447, 589, 712, 912
SsiI CCGC 4 cut(s) 270, 285, 759, 791
SspI AATATT 2 cut(s) 538, 692
SspMI CTAG 3 cut(s) 734, 801, 906
SstI GAGCTC 1 cut(s) 710
StyD4I CCNGG 1 cut(s) 857
TaaI ACNGT 3 cut(s) 24, 343, 513
TasI AATT 5 cut(s) 382, 447, 589, 712, 912
TatI WGTACW 1 cut(s) 797
TauI GCSGC 1 cut(s) 794
TfiI GAWTC 2 cut(s) 152, 730
Tru1I TTAA 6 cut(s) 12, 240, 291, 525, 636, 807
Tru9I TTAA 6 cut(s) 12, 240, 291, 525, 636, 807
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 4 cut(s) 53, 71, 272, 583
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 163, 290, 815
TspGWI ACGGA 1 cut(s) 246
XmiI GTMKAC 1 cut(s) 405
XspI CTAG 3 cut(s) 734, 801, 906
Zsp2I ATGCAT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.