Rh5BG418500

Glycosyl hydrolases family 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009671
Physical Location & Seq
Reverse (-)
67806218 .. 67807180
963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG418500.1

Sequence Viewer

Length: 963 bp
ATGAACTCAACCAGGCGGCAACTGGTTCTCTTCATTCAAATGACATTGTTCTTCACAATACTGTCATCAGCCCAAGCAGCCCCAACAAACTCGAAGCTCTTCAGGGAATACATAGGAGCAGAGGACACCGGTGTCACATTCTCAGATGTACCAATAGATTCCGGAGTCGAGTTTCACTTCATCCTCTCCTTTGCTATTGACTACACAGCATCTTCCCATCCAAAACCCGCCAATGGCACCTTTAACTTCACAACATCTTCCCATCCAAAACCCACCAATGGCAACTTTAATGTGTTCTGGGACACAAAGAATCTCAGCCCTTCTCATGTTTCTGCTATTAAGGCCAATCATCCAAATGCTAAAGTTGCTCTGAGTCTTGGAGGAGATACCGTAACCGGTAAACAGTATGCATACTTCAGTCCTACATCAATCAATTCTTGGGTCAGAAATGCTGTCACTTCAATAGTTCAGATAACTGAAAAATATAAGTTGGATGGAATTGATATTGACTATGAACACTTCGAGACAGATCCCAATACATTTGCTGAATGCATTGGGCAACTATTATTCCACCTTAAACATAATAATGTAGTATCCTTCACATCAATTGCACCGTATGACAATGATTCTGTGCAACCATATTACTTGGCATTGTGGAAGAAGTATGGGCACCTCATTGACTATGTTAACTTTCAGTTTTATGCCTACAATAAGAGCACAACAATAACTCAGTTTATGAAACATTTTGAGACCCAGAGCTCAAATTACAAAGGTGGCAAGATTCTGGTGAGCATTGGAACAGATAAAAGTGGGGGTTTGTCTCCTGAAAATGGGTTCTTTAAGGCTTGTGCTAGGCTTAGAAGTCAGGGCAAACTTCATGGCATTTTTATTTGGTCCGCAGACGACTCCAAGAAGGCTGATTTTCATTATGAAAAGCAATCACAAAGCTTTCTTGCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

320

Amino Acids

35.86

Weight (kDa)

7.78

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 101 - 238 4e-13 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AccB1I GGYRCC 2 cut(s) 236, 669
AccIII TCCGGA 1 cut(s) 161
AciI CCGC 3 cut(s) 16, 228, 897
AclWI GGATC 1 cut(s) 524
AcuI CTGAAG 2 cut(s) 85, 400
AfaI GTAC 1 cut(s) 150
AfiI CCNNNNNNNGG 3 cut(s) 233, 278, 830
AgeI ACCGGT 2 cut(s) 128, 395
AgsI TTSAA 2 cut(s) 38, 462
AjnI CCWGG 1 cut(s) 11
AluBI AGCT 4 cut(s) 97, 759, 948, 960
AluI AGCT 4 cut(s) 97, 759, 948, 960
Alw21I GWGCWC 2 cut(s) 719, 761
Alw26I GTCTC 3 cut(s) 518, 743, 825
AlwI GGATC 1 cut(s) 524
Aor13HI TCCGGA 1 cut(s) 161
AoxI GGCC 1 cut(s) 342
ApeKI GCWGC 1 cut(s) 77
AsiGI ACCGGT 2 cut(s) 128, 395
Asp700I GAANNNNTTC 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 894
AsuHPI GGTGA 1 cut(s) 799
AsuNHI GCTAGC 1 cut(s) 956
AvaII GGWCC 1 cut(s) 894
BaeGI GKGCMC 1 cut(s) 672
BanI GGYRCC 2 cut(s) 236, 669
BanII GRGCYC 1 cut(s) 761
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
Bbv12I GWGCWC 2 cut(s) 719, 761
BbvI GCAGC 1 cut(s) 89
BccI CCATC 3 cut(s) 225, 270, 488
BciT130I CCWGG 1 cut(s) 13
BciVI GTATCC 1 cut(s) 604
BcoDI GTCTC 3 cut(s) 518, 743, 825
BfaI CTAG 2 cut(s) 852, 957
BfuI GTATCC 1 cut(s) 604
BisI GCNGC 2 cut(s) 17, 78
BlsI GCNGC 2 cut(s) 18, 79
Bme1390I CCNGG 1 cut(s) 13
Bme18I GGWCC 1 cut(s) 894
BmgT120I GGNCC 1 cut(s) 894
BmiI GGNNCC 2 cut(s) 238, 671
BmrFI CCNGG 1 cut(s) 13
BmsI GCATC 1 cut(s) 218
BmtI GCTAGC 1 cut(s) 960
BsaI GGTCTC 1 cut(s) 743
BsaWI WCCGGW 3 cut(s) 128, 161, 395
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 278, 830
Bse118I RCCGGY 2 cut(s) 128, 395
Bse1I ACTGG 1 cut(s) 27
BseAI TCCGGA 1 cut(s) 161
BseBI CCWGG 1 cut(s) 13
BseGI GGATG 5 cut(s) 180, 217, 262, 349, 499
BseLI CCNNNNNNNGG 3 cut(s) 233, 278, 830
BseMII CTCAG 4 cut(s) 156, 328, 362, 743
BseNI ACTGG 1 cut(s) 27
BseRI GAGGAG 1 cut(s) 396
BseSI GKGCMC 1 cut(s) 672
BseXI GCAGC 1 cut(s) 89
BshFI GGCC 1 cut(s) 344
BshNI GGYRCC 2 cut(s) 236, 669
BshTI ACCGGT 2 cut(s) 128, 395
BsiHKAI GWGCWC 2 cut(s) 719, 761
BsiSI CCGG 3 cut(s) 129, 162, 396
BslFI GGGAC 1 cut(s) 314
BslI CCNNNNNNNGG 3 cut(s) 233, 278, 830
BsmAI GTCTC 3 cut(s) 518, 743, 825
BsmFI GGGAC 1 cut(s) 314
BsmI GAATGC 1 cut(s) 554
BsnI GGCC 1 cut(s) 344
Bso31I GGTCTC 1 cut(s) 743
Bsp1286I GDGCHC 3 cut(s) 672, 719, 761
Bsp13I TCCGGA 1 cut(s) 161
Bsp143I GATC 1 cut(s) 529
BspACI CCGC 3 cut(s) 16, 228, 897
BspANI GGCC 1 cut(s) 344
BspCNI CTCAG 4 cut(s) 155, 327, 363, 742
BspEI TCCGGA 1 cut(s) 161
BspLI GGNNCC 2 cut(s) 238, 671
BspOI GCTAGC 1 cut(s) 960
BspPI GGATC 1 cut(s) 524
BspQI GCTCTTC 1 cut(s) 104
BspT107I GGYRCC 2 cut(s) 236, 669
BspTNI GGTCTC 1 cut(s) 743
BsrFI RCCGGY 2 cut(s) 128, 395
BsrI ACTGG 1 cut(s) 27
BssAI RCCGGY 2 cut(s) 128, 395
BssMI GATC 1 cut(s) 529
Bst2UI CCWGG 1 cut(s) 13
Bst4CI ACNGT 4 cut(s) 63, 391, 405, 615
Bst6I CTCTTC 2 cut(s) 35, 104
BstC8I GCNNGC 1 cut(s) 958
BstDEI CTNAG 5 cut(s) 142, 314, 371, 729, 857
BstF5I GGATG 5 cut(s) 180, 217, 262, 349, 499
BstKTI GATC 1 cut(s) 532
BstMAI GTCTC 3 cut(s) 518, 743, 825
BstMBI GATC 1 cut(s) 529
BstMWI GCNNNNNNNGC 3 cut(s) 77, 341, 365
BstNI CCWGG 1 cut(s) 13
BstSCI CCNGG 1 cut(s) 11
BstSLI GKGCMC 1 cut(s) 672
BstV1I GCAGC 1 cut(s) 89
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 1 cut(s) 344
BtsCI GGATG 5 cut(s) 180, 217, 262, 349, 499
Cac8I GCNNGC 1 cut(s) 958
Cfr10I RCCGGY 2 cut(s) 128, 395
Cfr13I GGNCC 1 cut(s) 894
Csp6I GTAC 1 cut(s) 149
CspAI ACCGGT 2 cut(s) 128, 395
CviAII CATG 2 cut(s) 326, 878
CviQI GTAC 1 cut(s) 149
DdeI CTNAG 5 cut(s) 142, 314, 371, 729, 857
DpnI GATC 1 cut(s) 531
DpnII GATC 1 cut(s) 529
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
Eam1104I CTCTTC 2 cut(s) 35, 104
EarI CTCTTC 2 cut(s) 35, 104
Ecl136II GAGCTC 1 cut(s) 759
Eco24I GRGCYC 1 cut(s) 761
Eco31I GGTCTC 1 cut(s) 743
Eco47I GGWCC 1 cut(s) 894
Eco53kI GAGCTC 1 cut(s) 759
Eco57I CTGAAG 2 cut(s) 85, 400
EcoICRI GAGCTC 1 cut(s) 759
EcoRII CCWGG 1 cut(s) 11
EcoT22I ATGCAT 2 cut(s) 412, 554
EcoT38I GRGCYC 1 cut(s) 761
FaeI CATG 2 cut(s) 329, 881
FaqI GGGAC 1 cut(s) 314
FatI CATG 2 cut(s) 325, 877
FauI CCCGC 1 cut(s) 235
Fnu4HI GCNGC 2 cut(s) 17, 78
FokI GGATG 5 cut(s) 167, 204, 249, 336, 506
FriOI GRGCYC 1 cut(s) 761
Fsp4HI GCNGC 2 cut(s) 17, 78
FspBI CTAG 2 cut(s) 852, 957
GluI GCNGC 2 cut(s) 17, 78
HaeIII GGCC 1 cut(s) 344
HapII CCGG 3 cut(s) 129, 162, 396
Hin1II CATG 2 cut(s) 329, 881
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HindIII AAGCTT 1 cut(s) 946
HinfI GANTC 7 cut(s) 158, 165, 310, 373, 626, 781, 905
HpaI GTTAAC 1 cut(s) 688
HpaII CCGG 3 cut(s) 129, 162, 396
HphI GGTGA 1 cut(s) 799
Hpy166II GTNNAC 2 cut(s) 401, 688
Hpy188I TCNGA 4 cut(s) 145, 372, 446, 471
Hpy188III TCNNGA 3 cut(s) 162, 523, 824
Hpy8I GTNNAC 2 cut(s) 401, 688
HpyAV CCTTC 3 cut(s) 330, 607, 907
HpyCH4III ACNGT 4 cut(s) 63, 391, 405, 615
HpyCH4V TGCA 4 cut(s) 410, 552, 611, 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 341, 365
HpyF3I CTNAG 5 cut(s) 142, 314, 371, 729, 857
Hsp92II CATG 2 cut(s) 329, 881
Kpn2I TCCGGA 1 cut(s) 161
KspAI GTTAAC 1 cut(s) 688
Kzo9I GATC 1 cut(s) 529
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 1 cut(s) 116
Lsp1109I GCAGC 1 cut(s) 89
LweI GCATC 1 cut(s) 218
MaeI CTAG 2 cut(s) 852, 957
MaeIII GTNAC 3 cut(s) 133, 391, 454
MalI GATC 1 cut(s) 531
MboI GATC 1 cut(s) 529
MboII GAAGA 6 cut(s) 22, 43, 91, 204, 249, 670
MfeI CAATTG 1 cut(s) 606
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 3 cut(s) 672, 719, 761
MluCI AATT 4 cut(s) 433, 498, 606, 763
MlyI GAGTC 3 cut(s) 174, 382, 899
MmeI TCCRAC 1 cut(s) 471
MnlI CCTC 4 cut(s) 115, 194, 374, 683
Mph1103I ATGCAT 2 cut(s) 412, 554
MroI TCCGGA 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 6 cut(s) 243, 288, 339, 576, 687, 840
MslI CAYNNNNRTG 3 cut(s) 38, 354, 585
MspI CCGG 3 cut(s) 129, 162, 396
MspR9I CCNGG 1 cut(s) 13
MunI CAATTG 1 cut(s) 606
Mva1269I GAATGC 1 cut(s) 554
MvaI CCWGG 1 cut(s) 13
MwoI GCNNNNNNNGC 3 cut(s) 77, 341, 365
NdeII GATC 1 cut(s) 529
NheI GCTAGC 1 cut(s) 956
NlaIII CATG 2 cut(s) 329, 881
NlaIV GGNNCC 2 cut(s) 238, 671
NmuCI GTSAC 2 cut(s) 133, 454
NsiI ATGCAT 2 cut(s) 412, 554
PciSI GCTCTTC 1 cut(s) 104
PctI GAATGC 1 cut(s) 554
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 4 cut(s) 158, 310, 626, 781
PinAI ACCGGT 2 cut(s) 128, 395
PkrI GCNGC 2 cut(s) 18, 79
PleI GAGTC 3 cut(s) 173, 381, 899
PpsI GAGTC 3 cut(s) 173, 381, 899
Psp124BI GAGCTC 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 11
PspGI CCWGG 1 cut(s) 11
PspN4I GGNNCC 2 cut(s) 238, 671
PspPI GGNCC 1 cut(s) 894
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
RseI CAYNNNNRTG 3 cut(s) 38, 354, 585
SacI GAGCTC 1 cut(s) 761
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 6 cut(s) 243, 288, 339, 576, 687, 840
SatI GCNGC 2 cut(s) 17, 78
Sau3AI GATC 1 cut(s) 529
Sau96I GGNCC 1 cut(s) 894
SchI GAGTC 3 cut(s) 174, 382, 899
ScrFI CCNGG 1 cut(s) 13
SduI GDGCHC 3 cut(s) 672, 719, 761
SetI ASST 8 cut(s) 99, 242, 576, 675, 761, 775, 950, 962
SfaNI GCATC 1 cut(s) 218
SgrAI CRCCGGYG 1 cut(s) 128
SinI GGWCC 1 cut(s) 894
SmiMI CAYNNNNRTG 3 cut(s) 38, 354, 585
Sse9I AATT 4 cut(s) 433, 498, 606, 763
SsiI CCGC 3 cut(s) 16, 228, 897
SspMI CTAG 2 cut(s) 852, 957
SstI GAGCTC 1 cut(s) 761
StyD4I CCNGG 1 cut(s) 11
TaaI ACNGT 4 cut(s) 63, 391, 405, 615
TaqI TCGA 3 cut(s) 92, 168, 522
TasI AATT 4 cut(s) 433, 498, 606, 763
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 4 cut(s) 158, 310, 626, 781
Tru1I TTAA 6 cut(s) 243, 288, 339, 576, 687, 840
Tru9I TTAA 6 cut(s) 243, 288, 339, 576, 687, 840
TseFI GTSAC 2 cut(s) 133, 454
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 2 cut(s) 133, 454
TspDTI ATGAA 8 cut(s) 17, 22, 169, 528, 752, 866, 914, 945
VpaK11BI GGWCC 1 cut(s) 894
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 852, 957
Zsp2I ATGCAT 2 cut(s) 412, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.