FvH4_3g34320

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009671
Physical Location & Seq
Reverse (-)
29816560 .. 29817408
849 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34320.t1

Sequence Viewer

Length: 849 bp
ATGTCACTAGCCGTAGCAGCGAACTCGAAGCTCTTCAGGGAATACATAGGAGCCGAGGGCGTCGGTATCACATTCTCTGATGTACCAATTGATCCCGGAGTCGAGTTTCACTTCCTTCTTTCCTTTGGCATTGACTACACAACATCTTCCCGTCCTAAACCCACCAATGGCATCTTCAATGCGTTCTGGGACACAGATAATCTCAGCCCTTCTCATGTTTCAGCCATTAAGGCCCGTCATCGAAATGTTAAAGTAGGTCTGAGTCTTGGAGGAGATACCGTAGCTGGTGTTTCTGCAAACTTCACTCCTATATCAATCAACTCTTGGGTCAGAAATGCCATCTCTTCCATAGTAAAGATCACAAAAGAATACAAGTTGGATGGAATCGATATCGACTACGAACATTTCAACACAGATCCTAACACCTTTGCTGAATGCATTGGGCGACTATTTTTCTACCTGAAACAAAACAAAGTTGTGTCCTTCACATCAATAGCACCATATGACGATGATGCTGTGCAACCATATTACTTAGCATTGTGGAGAAAGTATGGGCATCTCATTGACTATGTTAACTTTCAGTTCTATGCCTATGATAAGGGCACAACTGTTACTCAGTTCATGAAATATTTTGGGACTCAGAGCTCAAATTACAAAGGGGGCAAGGTCCTAGTGAGCTTTGGAACAGACCAAAGTGGGGGTTTGTCACCTGAACATGGGTTCTTTGACGCTTGTGCTACGCTTAAAAGTCAGGGCAAGCTTAATGGTATTTTTATTTGGTCTGCAGACGACTCCATGAAGGCTCACTTTCGTTATGAGAAGCGATCACAAAGCTTACTTGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

283

Amino Acids

31.4

Weight (kDa)

7.05

Isoelectric Point (pI)

27.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 72 - 200 1.3e-13 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 86, 410
AcuI CTGAAG 1 cut(s) 19
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 84
AfiI CCNNNNNNNGG 3 cut(s) 167, 697, 716
AgsI TTSAA 2 cut(s) 178, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AluBI AGCT 7 cut(s) 31, 284, 645, 678, 760, 834, 846
AluI AGCT 7 cut(s) 31, 284, 645, 678, 760, 834, 846
Alw21I GWGCWC 1 cut(s) 647
AlwI GGATC 2 cut(s) 86, 410
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 32
AspS9I GGNCC 2 cut(s) 232, 667
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 699
AsuNHI GCTAGC 1 cut(s) 842
AvaII GGWCC 1 cut(s) 667
BaeGI GKGCMC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 647
Bbv12I GWGCWC 1 cut(s) 647
BbvI GCAGC 1 cut(s) 29
BccI CCATC 2 cut(s) 347, 374
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BcnI CCSGG 1 cut(s) 96
BfaI CTAG 4 cut(s) 8, 671, 843, 847
BfmI CTRYAG 1 cut(s) 783
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 1 cut(s) 667
BmgT120I GGNCC 2 cut(s) 232, 667
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 96
BmsI GCATC 3 cut(s) 180, 502, 565
BmtI GCTAGC 1 cut(s) 846
BpuMI CCSGG 1 cut(s) 96
Bsa29I ATCGAT 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 697, 716
BseCI ATCGAT 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 3 cut(s) 167, 697, 716
BseMII CTCAG 4 cut(s) 217, 251, 629, 653
BseRI GAGGAG 1 cut(s) 285
BseSI GKGCMC 1 cut(s) 605
BseXI GCAGC 1 cut(s) 29
BshFI GGCC 1 cut(s) 233
BshVI ATCGAT 1 cut(s) 387
BsiHKAI GWGCWC 1 cut(s) 647
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 2 cut(s) 203, 649
BslI CCNNNNNNNGG 3 cut(s) 167, 697, 716
BsmFI GGGAC 2 cut(s) 203, 649
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 605, 647
Bsp143I GATC 4 cut(s) 91, 357, 415, 824
BspANI GGCC 1 cut(s) 233
BspCNI CTCAG 4 cut(s) 216, 252, 628, 652
BspDI ATCGAT 1 cut(s) 387
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 1 cut(s) 52
BspMAI CTGCAG 1 cut(s) 787
BspOI GCTAGC 1 cut(s) 846
BspPI GGATC 2 cut(s) 86, 410
BspQI GCTCTTC 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 4 cut(s) 91, 357, 415, 824
BssNI GRCGYC 1 cut(s) 60
Bst4CI ACNGT 2 cut(s) 280, 610
Bst6I CTCTTC 2 cut(s) 38, 349
BstACI GRCGYC 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 758, 844
BstDEI CTNAG 5 cut(s) 203, 260, 532, 615, 639
BstF5I GGATG 1 cut(s) 385
BstKTI GATC 4 cut(s) 94, 360, 418, 827
BstMBI GATC 4 cut(s) 91, 357, 415, 824
BstMWI GCNNNNNNNGC 2 cut(s) 17, 230
BstSCI CCNGG 1 cut(s) 94
BstSFI CTRYAG 1 cut(s) 783
BstSLI GKGCMC 1 cut(s) 605
BstV1I GCAGC 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
Bsu15I ATCGAT 1 cut(s) 387
BsuRI GGCC 1 cut(s) 233
BsuTUI ATCGAT 1 cut(s) 387
BtsCI GGATG 1 cut(s) 385
Cac8I GCNNGC 2 cut(s) 758, 844
CciI TCATGA 1 cut(s) 621
Cfr13I GGNCC 2 cut(s) 232, 667
ClaI ATCGAT 1 cut(s) 387
CseI GACGC 2 cut(s) 49, 737
Csp6I GTAC 1 cut(s) 83
CviAII CATG 4 cut(s) 215, 622, 716, 796
CviQI GTAC 1 cut(s) 83
DdeI CTNAG 5 cut(s) 203, 260, 532, 615, 639
DpnI GATC 4 cut(s) 93, 359, 417, 826
DpnII GATC 4 cut(s) 91, 357, 415, 824
Eam1104I CTCTTC 2 cut(s) 38, 349
EarI CTCTTC 2 cut(s) 38, 349
Ecl136II GAGCTC 1 cut(s) 645
Eco24I GRGCYC 1 cut(s) 647
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 667
Eco53kI GAGCTC 1 cut(s) 645
Eco57I CTGAAG 1 cut(s) 19
EcoICRI GAGCTC 1 cut(s) 645
EcoO109I RGGNCCY 1 cut(s) 667
EcoRV GATATC 1 cut(s) 391
EcoT22I ATGCAT 1 cut(s) 440
EcoT38I GRGCYC 1 cut(s) 647
FaeI CATG 4 cut(s) 218, 625, 719, 799
FalI AAGNNNNNCTT 2 cut(s) 791, 823
FaqI GGGAC 2 cut(s) 203, 649
FatI CATG 4 cut(s) 214, 621, 715, 795
FauNDI CATATG 1 cut(s) 502
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 1 cut(s) 392
FriOI GRGCYC 1 cut(s) 647
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 4 cut(s) 8, 671, 843, 847
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 1 cut(s) 233
HapII CCGG 1 cut(s) 96
HgaI GACGC 2 cut(s) 49, 737
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 4 cut(s) 218, 625, 719, 799
HincII GTYRAC 1 cut(s) 574
HindII GTYRAC 1 cut(s) 574
HindIII AAGCTT 2 cut(s) 758, 832
HinfI GANTC 5 cut(s) 99, 262, 384, 637, 791
HpaI GTTAAC 1 cut(s) 574
HpaII CCGG 1 cut(s) 96
HphI GGTGA 1 cut(s) 699
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 4 cut(s) 79, 261, 332, 642
Hpy188III TCNNGA 1 cut(s) 622
Hpy8I GTNNAC 1 cut(s) 574
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 4 cut(s) 125, 219, 493, 793
HpyCH4III ACNGT 2 cut(s) 280, 610
HpyCH4V TGCA 4 cut(s) 296, 438, 520, 785
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 230
HpyF3I CTNAG 5 cut(s) 203, 260, 532, 615, 639
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 4 cut(s) 218, 625, 719, 799
KspAI GTTAAC 1 cut(s) 574
Kzo9I GATC 4 cut(s) 91, 357, 415, 824
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 1 cut(s) 50
LpnPI CCDG 7 cut(s) 22, 109, 172, 270, 473, 723, 737
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 3 cut(s) 180, 502, 565
MaeI CTAG 4 cut(s) 8, 671, 843, 847
MaeIII GTNAC 3 cut(s) 3, 610, 705
MalI GATC 4 cut(s) 93, 359, 417, 826
MboI GATC 4 cut(s) 91, 357, 415, 824
MboII GAAGA 4 cut(s) 25, 138, 166, 336
MfeI CAATTG 1 cut(s) 87
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 2 cut(s) 605, 647
MluCI AATT 2 cut(s) 87, 649
MlyI GAGTC 4 cut(s) 108, 271, 631, 785
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 2 cut(s) 49, 263
Mph1103I ATGCAT 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 5 cut(s) 228, 249, 573, 744, 762
MslI CAYNNNNRTG 1 cut(s) 243
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 1 cut(s) 96
MunI CAATTG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 17, 230
NciI CCSGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 502
NdeII GATC 4 cut(s) 91, 357, 415, 824
NheI GCTAGC 1 cut(s) 842
NlaIII CATG 4 cut(s) 218, 625, 719, 799
NlaIV GGNNCC 1 cut(s) 52
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 2 cut(s) 3, 705
NsiI ATGCAT 1 cut(s) 440
PagI TCATGA 1 cut(s) 621
PciSI GCTCTTC 1 cut(s) 38
PctI GAATGC 1 cut(s) 440
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 1 cut(s) 384
PfoI TCCNGGA 1 cut(s) 94
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 4 cut(s) 107, 270, 631, 785
PpsI GAGTC 4 cut(s) 107, 270, 631, 785
PpuMI RGGWCCY 1 cut(s) 667
Psp124BI GAGCTC 1 cut(s) 647
Psp5II RGGWCCY 1 cut(s) 667
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 2 cut(s) 232, 667
PspPPI RGGWCCY 1 cut(s) 667
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 1 cut(s) 84
RsaNI GTAC 1 cut(s) 83
RseI CAYNNNNRTG 1 cut(s) 243
SacI GAGCTC 1 cut(s) 647
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 5 cut(s) 228, 249, 573, 744, 762
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 4 cut(s) 91, 357, 415, 824
Sau96I GGNCC 2 cut(s) 232, 667
SchI GAGTC 4 cut(s) 108, 271, 631, 785
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 2 cut(s) 605, 647
SfaNI GCATC 3 cut(s) 180, 502, 565
SfcI CTRYAG 1 cut(s) 783
SinI GGWCC 1 cut(s) 667
SmiMI CAYNNNNRTG 1 cut(s) 243
Sse9I AATT 2 cut(s) 87, 649
SspI AATATT 1 cut(s) 629
SspMI CTAG 4 cut(s) 8, 671, 843, 847
SstI GAGCTC 1 cut(s) 647
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 2 cut(s) 280, 610
TaqI TCGA 5 cut(s) 26, 102, 241, 387, 393
TasI AATT 2 cut(s) 87, 649
TfiI GAWTC 1 cut(s) 384
Tru1I TTAA 5 cut(s) 228, 249, 573, 744, 762
Tru9I TTAA 5 cut(s) 228, 249, 573, 744, 762
TseFI GTSAC 2 cut(s) 3, 705
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 2 cut(s) 3, 705
TspDTI ATGAA 3 cut(s) 610, 638, 812
VpaK11BI GGWCC 1 cut(s) 667
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 4 cut(s) 8, 671, 843, 847
Zsp2I ATGCAT 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.