RLG00000035513

Glycosyl hydrolases family 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009671
Physical Location & Seq
Reverse (-)
67889098 .. 67890015
918 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035513

Sequence Viewer

Length: 918 bp
ATGAACTCAACCAGGCTGCAACTGGTTCTCTTCCTTCAAATGACAACGTTCTTCACAATACTGTCATCAGCCCAAGCAGCCCCAACAAACTCGAAGCTCTTCAGGGAATACATAGGAGCCGAGGACACCGGTGTCACATTCTCAGATGTACCAATAGATTCCGGAGTCGAGTTTCACTTCATGCTTTCCTTTGCTATTGACTACACAGCATCTTCCCATCCAAAACCCGCCAATGGCAACTTTAGTGTGTTCTGGGACACAAAGAATCTCAGCCCTTCTCATGTTTCTGCTATTAAGGCCAATCATCCAAACGCTAAAGTTGCTCTGAGTCTTGGAGGAGATACCGTAACCGGTAAACAGTATGCATACTTTAGTCCTACATCAATCAATTCTTGGGTCAGAAATGCTATCGCTTCAATAGTTCAGATAACTGAAAATTATAAGTTGGATGGAATTGATATTGACTATGAACACTTCCAGACAGATCCCAATACATTTGCTGAATGCATTGGGCAACTATTATTCCACCTTAAATATAATAATGTAGTGTCCTTCACATCAATTGCACCGTATGACAATGATTCTGTGCAACCATATTACTTGGCATTGTGGAAGAAGTATGGGCACCTCATTGACTATGTTAACTTTCAGTTTTATGCCTACAATAAGGGCACAACAATAACTCAGTTTATGAAACATTTTGAGACCCAGAGCTCAAATTACAAAGGTGGCAAGATCCTGGTGAGCATTGGAACAGATAAAAGTGGGGGTTTGTCTCCTGAAAATGGGTTCTTTAAGGCTTGTGCTAGGCTTAGAAGTCAGGGCAAGCTTCATGGCATTTTTATTTGGTCCGCAGACGACTCCAAGAAGGCCGATTTTCGTTATGAAAAGCAATCACAAAGCTTTCTTGCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

306

Amino Acids

34.16

Weight (kDa)

7.12

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 84 - 222 6.4e-13 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 441
AasI GACNNNNNNGTC 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 624
AccIII TCCGGA 1 cut(s) 161
AciI CCGC 2 cut(s) 228, 852
AclI AACGTT 1 cut(s) 47
AclWI GGATC 2 cut(s) 479, 730
AcuI CTGAAG 1 cut(s) 85
AfaI GTAC 1 cut(s) 150
AfiI CCNNNNNNNGG 2 cut(s) 233, 785
AgeI ACCGGT 2 cut(s) 128, 350
AgsI TTSAA 2 cut(s) 38, 417
AjnI CCWGG 2 cut(s) 11, 738
AluBI AGCT 5 cut(s) 97, 714, 829, 903, 915
AluI AGCT 5 cut(s) 97, 714, 829, 903, 915
Alw21I GWGCWC 1 cut(s) 716
Alw26I GTCTC 2 cut(s) 698, 780
AlwI GGATC 2 cut(s) 479, 730
Aor13HI TCCGGA 1 cut(s) 161
AoxI GGCC 2 cut(s) 297, 870
ApeKI GCWGC 2 cut(s) 16, 77
AsiGI ACCGGT 2 cut(s) 128, 350
Asp700I GAANNNNTTC 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 849
AsuHPI GGTGA 1 cut(s) 754
AsuNHI GCTAGC 1 cut(s) 911
AvaII GGWCC 1 cut(s) 849
BaeGI GKGCMC 2 cut(s) 627, 674
BanI GGYRCC 1 cut(s) 624
BanII GRGCYC 1 cut(s) 716
Bbv12I GWGCWC 1 cut(s) 716
BbvI GCAGC 2 cut(s) 3, 89
BccI CCATC 2 cut(s) 225, 443
BciT130I CCWGG 2 cut(s) 13, 740
BcoDI GTCTC 2 cut(s) 698, 780
BfaI CTAG 2 cut(s) 807, 912
BisI GCNGC 2 cut(s) 17, 78
BlsI GCNGC 2 cut(s) 18, 79
Bme1390I CCNGG 2 cut(s) 13, 740
Bme18I GGWCC 1 cut(s) 849
BmgT120I GGNCC 1 cut(s) 849
BmiI GGNNCC 2 cut(s) 118, 626
BmrFI CCNGG 2 cut(s) 13, 740
BmsI GCATC 1 cut(s) 218
BmtI GCTAGC 1 cut(s) 915
BsaI GGTCTC 1 cut(s) 698
BsaJI CCNNGG 1 cut(s) 120
BsaWI WCCGGW 3 cut(s) 128, 161, 350
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 785
Bse118I RCCGGY 2 cut(s) 128, 350
Bse1I ACTGG 1 cut(s) 27
BseAI TCCGGA 1 cut(s) 161
BseBI CCWGG 2 cut(s) 13, 740
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 3 cut(s) 217, 304, 454
BseLI CCNNNNNNNGG 2 cut(s) 233, 785
BseMII CTCAG 4 cut(s) 156, 283, 317, 698
BseNI ACTGG 1 cut(s) 27
BseRI GAGGAG 1 cut(s) 351
BseSI GKGCMC 2 cut(s) 627, 674
BseXI GCAGC 2 cut(s) 3, 89
BshFI GGCC 2 cut(s) 299, 872
BshNI GGYRCC 1 cut(s) 624
BshTI ACCGGT 2 cut(s) 128, 350
BsiHKAI GWGCWC 1 cut(s) 716
BsiSI CCGG 3 cut(s) 129, 162, 351
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 233, 785
BsmAI GTCTC 2 cut(s) 698, 780
BsmFI GGGAC 1 cut(s) 269
BsmI GAATGC 1 cut(s) 509
BsnI GGCC 2 cut(s) 299, 872
Bso31I GGTCTC 1 cut(s) 698
Bsp1286I GDGCHC 3 cut(s) 627, 674, 716
Bsp13I TCCGGA 1 cut(s) 161
Bsp143I GATC 2 cut(s) 484, 735
BspACI CCGC 2 cut(s) 228, 852
BspANI GGCC 2 cut(s) 299, 872
BspCNI CTCAG 4 cut(s) 155, 282, 318, 697
BspEI TCCGGA 1 cut(s) 161
BspLI GGNNCC 2 cut(s) 118, 626
BspOI GCTAGC 1 cut(s) 915
BspPI GGATC 2 cut(s) 479, 730
BspQI GCTCTTC 1 cut(s) 104
BspT107I GGYRCC 1 cut(s) 624
BspTNI GGTCTC 1 cut(s) 698
BsrFI RCCGGY 2 cut(s) 128, 350
BsrI ACTGG 1 cut(s) 27
BssAI RCCGGY 2 cut(s) 128, 350
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 2 cut(s) 484, 735
Bst2UI CCWGG 2 cut(s) 13, 740
Bst4CI ACNGT 4 cut(s) 63, 346, 360, 570
Bst6I CTCTTC 2 cut(s) 35, 104
BstC8I GCNNGC 2 cut(s) 827, 913
BstDEI CTNAG 5 cut(s) 142, 269, 326, 684, 812
BstF5I GGATG 3 cut(s) 217, 304, 454
BstKTI GATC 2 cut(s) 487, 738
BstMAI GTCTC 2 cut(s) 698, 780
BstMBI GATC 2 cut(s) 484, 735
BstMWI GCNNNNNNNGC 3 cut(s) 77, 296, 320
BstNI CCWGG 2 cut(s) 13, 740
BstSCI CCNGG 2 cut(s) 11, 738
BstSLI GKGCMC 2 cut(s) 627, 674
BstV1I GCAGC 2 cut(s) 3, 89
BstX2I RGATCY 2 cut(s) 484, 735
BstYI RGATCY 2 cut(s) 484, 735
BsuRI GGCC 2 cut(s) 299, 872
BtsCI GGATG 3 cut(s) 217, 304, 454
Cac8I GCNNGC 2 cut(s) 827, 913
Cfr10I RCCGGY 2 cut(s) 128, 350
Cfr13I GGNCC 1 cut(s) 849
Csp6I GTAC 1 cut(s) 149
CspAI ACCGGT 2 cut(s) 128, 350
CviAII CATG 3 cut(s) 181, 281, 833
CviQI GTAC 1 cut(s) 149
DdeI CTNAG 5 cut(s) 142, 269, 326, 684, 812
DpnI GATC 2 cut(s) 486, 737
DpnII GATC 2 cut(s) 484, 735
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
Eam1104I CTCTTC 2 cut(s) 35, 104
EarI CTCTTC 2 cut(s) 35, 104
Ecl136II GAGCTC 1 cut(s) 714
Eco24I GRGCYC 1 cut(s) 716
Eco31I GGTCTC 1 cut(s) 698
Eco47I GGWCC 1 cut(s) 849
Eco53kI GAGCTC 1 cut(s) 714
Eco57I CTGAAG 1 cut(s) 85
EcoICRI GAGCTC 1 cut(s) 714
EcoRII CCWGG 2 cut(s) 11, 738
EcoT22I ATGCAT 2 cut(s) 367, 509
EcoT38I GRGCYC 1 cut(s) 716
FaeI CATG 3 cut(s) 184, 284, 836
FaqI GGGAC 1 cut(s) 269
FatI CATG 3 cut(s) 180, 280, 832
FauI CCCGC 1 cut(s) 235
Fnu4HI GCNGC 2 cut(s) 17, 78
FokI GGATG 3 cut(s) 204, 291, 461
FriOI GRGCYC 1 cut(s) 716
Fsp4HI GCNGC 2 cut(s) 17, 78
FspBI CTAG 2 cut(s) 807, 912
GluI GCNGC 2 cut(s) 17, 78
HaeIII GGCC 2 cut(s) 299, 872
HapII CCGG 3 cut(s) 129, 162, 351
Hin1II CATG 3 cut(s) 184, 284, 836
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HindIII AAGCTT 2 cut(s) 827, 901
HinfI GANTC 6 cut(s) 158, 165, 265, 328, 581, 860
HpaI GTTAAC 1 cut(s) 643
HpaII CCGG 3 cut(s) 129, 162, 351
HphI GGTGA 1 cut(s) 754
Hpy166II GTNNAC 2 cut(s) 356, 643
Hpy188I TCNGA 4 cut(s) 145, 327, 401, 426
Hpy188III TCNNGA 3 cut(s) 162, 478, 779
Hpy8I GTNNAC 2 cut(s) 356, 643
HpyAV CCTTC 4 cut(s) 44, 285, 562, 862
HpyCH4III ACNGT 4 cut(s) 63, 346, 360, 570
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 5 cut(s) 19, 365, 507, 566, 589
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 296, 320
HpyF3I CTNAG 5 cut(s) 142, 269, 326, 684, 812
HpySE526I ACGT 1 cut(s) 47
Hsp92II CATG 3 cut(s) 184, 284, 836
Kpn2I TCCGGA 1 cut(s) 161
KspAI GTTAAC 1 cut(s) 643
Kzo9I GATC 2 cut(s) 484, 735
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 1 cut(s) 116
Lsp1109I GCAGC 2 cut(s) 3, 89
LweI GCATC 1 cut(s) 218
MaeI CTAG 2 cut(s) 807, 912
MaeII ACGT 1 cut(s) 47
MaeIII GTNAC 2 cut(s) 133, 346
MalI GATC 2 cut(s) 486, 737
MboI GATC 2 cut(s) 484, 735
MboII GAAGA 5 cut(s) 22, 43, 91, 204, 625
MfeI CAATTG 1 cut(s) 561
MflI RGATCY 2 cut(s) 484, 735
MhlI GDGCHC 3 cut(s) 627, 674, 716
MluCI AATT 5 cut(s) 388, 436, 453, 561, 718
MlyI GAGTC 3 cut(s) 174, 337, 854
MmeI TCCRAC 1 cut(s) 426
MnlI CCTC 3 cut(s) 115, 329, 638
Mph1103I ATGCAT 2 cut(s) 367, 509
MroI TCCGGA 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 4 cut(s) 294, 531, 642, 795
MspI CCGG 3 cut(s) 129, 162, 351
MspR9I CCNGG 2 cut(s) 13, 740
MunI CAATTG 1 cut(s) 561
Mva1269I GAATGC 1 cut(s) 509
MvaI CCWGG 2 cut(s) 13, 740
MwoI GCNNNNNNNGC 3 cut(s) 77, 296, 320
NdeII GATC 2 cut(s) 484, 735
NheI GCTAGC 1 cut(s) 911
NlaIII CATG 3 cut(s) 184, 284, 836
NlaIV GGNNCC 2 cut(s) 118, 626
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 2 cut(s) 367, 509
PciSI GCTCTTC 1 cut(s) 104
PctI GAATGC 1 cut(s) 509
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 3 cut(s) 158, 265, 581
PinAI ACCGGT 2 cut(s) 128, 350
PkrI GCNGC 2 cut(s) 18, 79
PleI GAGTC 3 cut(s) 173, 336, 854
PpsI GAGTC 3 cut(s) 173, 336, 854
PsiI TTATAA 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 716
Psp1406I AACGTT 1 cut(s) 47
Psp6I CCWGG 2 cut(s) 11, 738
PspGI CCWGG 2 cut(s) 11, 738
PspN4I GGNNCC 2 cut(s) 118, 626
PspPI GGNCC 1 cut(s) 849
PsuI RGATCY 2 cut(s) 484, 735
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
SacI GAGCTC 1 cut(s) 716
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 4 cut(s) 294, 531, 642, 795
SatI GCNGC 2 cut(s) 17, 78
Sau3AI GATC 2 cut(s) 484, 735
Sau96I GGNCC 1 cut(s) 849
SchI GAGTC 3 cut(s) 174, 337, 854
ScrFI CCNGG 2 cut(s) 13, 740
SduI GDGCHC 3 cut(s) 627, 674, 716
SetI ASST 9 cut(s) 50, 99, 531, 630, 716, 730, 831, 905, 917
SfaNI GCATC 1 cut(s) 218
SgrAI CRCCGGYG 1 cut(s) 128
SinI GGWCC 1 cut(s) 849
Sse9I AATT 5 cut(s) 388, 436, 453, 561, 718
SsiI CCGC 2 cut(s) 228, 852
SspMI CTAG 2 cut(s) 807, 912
SstI GAGCTC 1 cut(s) 716
StyD4I CCNGG 2 cut(s) 11, 738
TaaI ACNGT 4 cut(s) 63, 346, 360, 570
TaiI ACGT 1 cut(s) 50
TaqI TCGA 2 cut(s) 92, 168
TasI AATT 5 cut(s) 388, 436, 453, 561, 718
TfiI GAWTC 3 cut(s) 158, 265, 581
Tru1I TTAA 4 cut(s) 294, 531, 642, 795
Tru9I TTAA 4 cut(s) 294, 531, 642, 795
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 2 cut(s) 16, 77
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 6 cut(s) 17, 169, 483, 707, 821, 900
VpaK11BI GGWCC 1 cut(s) 849
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 807, 912
Zsp2I ATGCAT 2 cut(s) 367, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.