FvH4_3g35650

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007649
Physical Location & Seq
Reverse (-)
30593355 .. 30597847
4493 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g35650.t2

Sequence Viewer

Length: 504 bp
ATGGCGGCCTCTCAAGCGAGCCTTCTCCTCCAGAAGCAGCTCAAAGATCTTTGCAAGAACCCTGTGGATGGGTTCTCGGCGGGGCTGGTCGATGAGAACAATATCTTTGAATGGAGCGTCACAATTATTGGACCTCCCGATACGCTATATGAGGGAGGATTTTTCAACGCCATCATGAGTTTTCCTTCTAATTACCCAAACAGCCCTCCAACAGTGAAGTTTACTTCAGAGTTATGGCATCCCAATGTTTATCCTGATGGGCGCGTTTGCATTTCCATTCTACATCCTCCTGGTGATGATCCAAATGGTTATGAGCTTGCAAGTGAGCGTTGGACGCCTGTGCATACGGTTGAAAGCATAGTGCTGAGTATAATATCAATGCTGTCCAGTCCAAATGACGAATCTCCTGCAAATGTCGAAGCTGCTAAGGAATGGAGAGATCGGAGAGATGATTTCAAGAAGAAGGTCGGCCGTTGCGTGAGAAAGTCACAAGAGATGTTGTGA

Protein Analysis

168

Amino Acids

18.65

Weight (kDa)

5.04

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 10 - 146 2e-46 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 264
AciI CCGC 2 cut(s) 5, 80
AclWI GGATC 1 cut(s) 293
AcoI YGGCCR 1 cut(s) 469
AcuI CTGAAG 1 cut(s) 210
AcyI GRCGYC 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 68
AgsI TTSAA 4 cut(s) 110, 166, 353, 457
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 3 cut(s) 40, 316, 422
AluI AGCT 3 cut(s) 40, 316, 422
AlwI GGATC 1 cut(s) 293
AoxI GGCC 2 cut(s) 6, 469
ApeKI GCWGC 2 cut(s) 37, 422
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 1 cut(s) 305
AvaII GGWCC 1 cut(s) 131
BbvI GCAGC 2 cut(s) 49, 409
BccI CCATC 3 cut(s) 62, 179, 251
BceAI ACGGC 1 cut(s) 456
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 1 cut(s) 291
BglII AGATCT 1 cut(s) 46
BisI GCNGC 3 cut(s) 6, 38, 423
BlsI GCNGC 3 cut(s) 7, 39, 424
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 131
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 1 cut(s) 247
BpmI CTGGAG 1 cut(s) 14
Bpu10I CCTNAGC 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 335
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse1I ACTGG 1 cut(s) 387
BseBI CCWGG 1 cut(s) 291
BseGI GGATG 3 cut(s) 73, 238, 283
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 387
BseRI GAGGAG 1 cut(s) 17
BseX3I CGGCCG 1 cut(s) 469
BseXI GCAGC 2 cut(s) 49, 409
Bsh1236I CGCG 1 cut(s) 264
Bsh1285I CGRYCG 1 cut(s) 472
BshFI GGCC 2 cut(s) 8, 471
BsiEI CGRYCG 1 cut(s) 472
BslI CCNNNNNNNGG 1 cut(s) 68
BsnI GGCC 2 cut(s) 8, 471
Bsp143I GATC 3 cut(s) 46, 298, 439
BspACI CCGC 2 cut(s) 5, 80
BspANI GGCC 2 cut(s) 8, 471
BspCNI CTCAG 1 cut(s) 357
BspFNI CGCG 1 cut(s) 264
BspHI TCATGA 1 cut(s) 174
BspPI GGATC 1 cut(s) 293
BsrI ACTGG 1 cut(s) 387
BssMI GATC 3 cut(s) 46, 298, 439
BssNI GRCGYC 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 214, 349
BstACI GRCGYC 1 cut(s) 335
BstC8I GCNNGC 2 cut(s) 19, 318
BstDEI CTNAG 2 cut(s) 365, 426
BstF5I GGATG 3 cut(s) 73, 238, 283
BstFNI CGCG 1 cut(s) 264
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 3 cut(s) 49, 301, 442
BstMBI GATC 3 cut(s) 46, 298, 439
BstMCI CGRYCG 1 cut(s) 472
BstMWI GCNNNNNNNGC 2 cut(s) 14, 334
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 49, 409
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BstZI CGGCCG 1 cut(s) 469
BsuRI GGCC 2 cut(s) 8, 471
BtsCI GGATG 3 cut(s) 73, 238, 283
BtsIMutI CAGTG 1 cut(s) 219
Cac8I GCNNGC 2 cut(s) 19, 318
CciI TCATGA 1 cut(s) 174
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 131
CseI GACGC 2 cut(s) 106, 343
CviAII CATG 1 cut(s) 175
CviJI RGCY 8 cut(s) 8, 21, 40, 85, 204, 316, 422, 471
CviKI_1 RGCY 8 cut(s) 8, 21, 40, 85, 204, 316, 422, 471
DdeI CTNAG 2 cut(s) 365, 426
DpnI GATC 3 cut(s) 48, 300, 441
DpnII GATC 3 cut(s) 46, 298, 439
EaeI YGGCCR 1 cut(s) 469
EagI CGGCCG 1 cut(s) 469
EclXI CGGCCG 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 131
Eco52I CGGCCG 1 cut(s) 469
Eco57I CTGAAG 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 289
FaeI CATG 1 cut(s) 178
FaiI YATR 8 cut(s) 148, 150, 176, 235, 312, 345, 359, 371
FalI AAGNNNNNCTT 1 cut(s) 38
FatI CATG 1 cut(s) 174
FauI CCCGC 1 cut(s) 73
Fnu4HI GCNGC 3 cut(s) 6, 38, 423
FokI GGATG 3 cut(s) 80, 225, 270
Fsp4HI GCNGC 3 cut(s) 6, 38, 423
GlaI GCGC 1 cut(s) 263
GluI GCNGC 3 cut(s) 6, 38, 423
GsuI CTGGAG 1 cut(s) 14
HaeIII GGCC 2 cut(s) 8, 471
HgaI GACGC 2 cut(s) 106, 343
HhaI GCGC 1 cut(s) 264
Hin1I GRCGYC 1 cut(s) 335
Hin1II CATG 1 cut(s) 178
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HinfI GANTC 1 cut(s) 401
HphI GGTGA 1 cut(s) 305
Hpy166II GTNNAC 1 cut(s) 222
Hpy188I TCNGA 2 cut(s) 229, 444
Hpy188III TCNNGA 5 cut(s) 31, 137, 175, 254, 457
Hpy8I GTNNAC 1 cut(s) 222
HpyAV CCTTC 3 cut(s) 32, 195, 457
HpyCH4III ACNGT 2 cut(s) 214, 349
HpyCH4V TGCA 5 cut(s) 54, 270, 320, 343, 410
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 334
HpyF3I CTNAG 2 cut(s) 365, 426
Hsp92I GRCGYC 1 cut(s) 335
Hsp92II CATG 1 cut(s) 178
HspAI GCGC 1 cut(s) 262
Kzo9I GATC 3 cut(s) 46, 298, 439
LmnI GCTCC 1 cut(s) 114
LpnPI CCDG 9 cut(s) 44, 71, 75, 267, 276, 303, 351, 400, 420
Lsp1109I GCAGC 2 cut(s) 49, 409
LweI GCATC 1 cut(s) 247
MaeIII GTNAC 2 cut(s) 118, 486
MalI GATC 3 cut(s) 48, 300, 441
MboI GATC 3 cut(s) 46, 298, 439
MboII GAAGA 1 cut(s) 472
MflI RGATCY 1 cut(s) 46
MluCI AATT 2 cut(s) 123, 190
MmeI TCCRAC 2 cut(s) 233, 311
MnlI CCTC 7 cut(s) 19, 38, 144, 145, 149, 216, 297
MslI CAYNNNNRTG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 291
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 2 cut(s) 14, 334
NdeII GATC 3 cut(s) 46, 298, 439
NlaIII CATG 1 cut(s) 178
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 2 cut(s) 118, 486
PagI TCATGA 1 cut(s) 174
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PfeI GAWTC 1 cut(s) 401
PkrI GCNGC 3 cut(s) 7, 39, 424
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspPI GGNCC 1 cut(s) 131
PsuI RGATCY 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 243
SatI GCNGC 3 cut(s) 6, 38, 423
Sau3AI GATC 3 cut(s) 46, 298, 439
Sau96I GGNCC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 291
SetI ASST 5 cut(s) 42, 136, 318, 424, 468
SfaNI GCATC 1 cut(s) 247
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 243
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
Sse9I AATT 2 cut(s) 123, 190
SsiI CCGC 2 cut(s) 5, 80
StyD4I CCNGG 1 cut(s) 289
TaaI ACNGT 2 cut(s) 214, 349
TaqI TCGA 2 cut(s) 90, 417
TasI AATT 2 cut(s) 123, 190
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 401
TscAI CASTG 1 cut(s) 219
TseFI GTSAC 2 cut(s) 118, 486
TseI GCWGC 2 cut(s) 37, 422
Tsp45I GTSAC 2 cut(s) 118, 486
TspRI CASTG 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.