MD00G1137800.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007649
Physical Location & Seq
Forward (+)
29840025 .. 29844393
4369 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1137800.v1.1.491

Sequence Viewer

Length: 504 bp
ATGGCGGCGACGCAGGCGAGTCTTCTCCTCCAGAAACAGCTCAAGGATCTTTGCAAGAACCCGGTCGATGGATTCTCGGCCGGTCTGGTCGATGAAAACAATATCTTTGAGTGGAGCGTCACGATTATCGGACCGCCTGATACGCTCTATGAGGGGGGGTTTTTCAATGCCATCATGAGCTTTCCAAATAATTATCCGAACAGCCCTCCGACGGTTAAGTTTACTTCAGAGATATGGCATCCAAATGTTTATACTGATGGGCGTGTTTGCATATCTATTCTTCACCCCCCTGGTGAGGACCCAAATGGTTATGAGCTTGCAAGTGAGCGCTGGACGCCTGTCCATACGGTTGAAAGCATAGTCTTGAGTATAATATCAATGCTATCCAGCCCAAATGATGAATCTCCTGCAAATGTTGAAGCTGCAAAACAGTGGAGAGATCGGAGAGAGGAATTCAAGAAAAAGGTCAGCCGCTGCGTGAGGAAATCACAGGAGATGCTATGA

Protein Analysis

168

Amino Acids

18.77

Weight (kDa)

5.21

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 10 - 159 7e-53 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 134, 472
AclWI GGATC 1 cut(s) 54
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 210
AcyI GRCGYC 1 cut(s) 335
AfeI AGCGCT 1 cut(s) 329
AfiI CCNNNNNNNGG 3 cut(s) 68, 211, 295
AgsI TTSAA 4 cut(s) 166, 353, 419, 457
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 4 cut(s) 40, 180, 316, 422
AluI AGCT 4 cut(s) 40, 180, 316, 422
AlwI GGATC 1 cut(s) 54
AlwNI CAGNNNCTG 1 cut(s) 474
Aor51HI AGCGCT 1 cut(s) 329
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 2 cut(s) 422, 474
ApoI RAATTY 1 cut(s) 452
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 2 cut(s) 131, 298
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 275, 305
AvaII GGWCC 2 cut(s) 131, 298
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 409, 461
BccI CCATC 3 cut(s) 62, 179, 251
BciT130I CCWGG 1 cut(s) 291
BcnI CCSGG 1 cut(s) 62
BfoI RGCGCY 1 cut(s) 331
BisI GCNGC 4 cut(s) 6, 423, 472, 475
BlsI GCNGC 4 cut(s) 7, 424, 473, 476
Bme1390I CCNGG 2 cut(s) 62, 291
Bme18I GGWCC 2 cut(s) 131, 298
BmgT120I GGNCC 2 cut(s) 131, 298
BmiI GGNNCC 1 cut(s) 300
BmrFI CCNGG 2 cut(s) 62, 291
BmsI GCATC 2 cut(s) 247, 486
BoxI GACNNNNGTC 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 14
BpuEI CTTGAG 2 cut(s) 26, 385
BpuMI CCSGG 1 cut(s) 62
BsaHI GRCGYC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 289
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 211, 295
Bse118I RCCGGY 1 cut(s) 80
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 289
BseGI GGATG 1 cut(s) 238
BseLI CCNNNNNNNGG 3 cut(s) 68, 211, 295
BseRI GAGGAG 1 cut(s) 17
BseX3I CGGCCG 1 cut(s) 78
BseXI GCAGC 2 cut(s) 409, 461
Bsh1285I CGRYCG 2 cut(s) 66, 81
BshFI GGCC 1 cut(s) 80
BsiEI CGRYCG 2 cut(s) 66, 81
BsiSI CCGG 2 cut(s) 62, 81
BslI CCNNNNNNNGG 3 cut(s) 68, 211, 295
BsnI GGCC 1 cut(s) 80
Bsp143I GATC 2 cut(s) 46, 439
BspACI CCGC 3 cut(s) 5, 134, 472
BspANI GGCC 1 cut(s) 80
BspHI TCATGA 1 cut(s) 174
BspLI GGNNCC 1 cut(s) 300
BspPI GGATC 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 289
BssMI GATC 2 cut(s) 46, 439
BssNI GRCGYC 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 3 cut(s) 214, 349, 432
BstACI GRCGYC 1 cut(s) 335
BstC8I GCNNGC 2 cut(s) 15, 318
BstF5I GGATG 1 cut(s) 238
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 2 cut(s) 49, 442
BstMBI GATC 2 cut(s) 46, 439
BstMCI CGRYCG 2 cut(s) 66, 81
BstMWI GCNNNNNNNGC 3 cut(s) 14, 142, 334
BstNI CCWGG 1 cut(s) 291
BstPAI GACNNNNGTC 1 cut(s) 338
BstSCI CCNGG 2 cut(s) 60, 289
BstV1I GCAGC 2 cut(s) 409, 461
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BstZI CGGCCG 1 cut(s) 78
BsuRI GGCC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 238
BtsIMutI CAGTG 1 cut(s) 437
Cac8I GCNNGC 2 cut(s) 15, 318
CaiI CAGNNNCTG 1 cut(s) 474
CciI TCATGA 1 cut(s) 174
CfoI GCGC 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 131, 298
CpoI CGGWCCG 1 cut(s) 131
CseI GACGC 3 cut(s) 19, 106, 343
CspI CGGWCCG 1 cut(s) 131
CviAII CATG 1 cut(s) 175
CviJI RGCY 8 cut(s) 40, 80, 180, 204, 316, 390, 422, 471
CviKI_1 RGCY 8 cut(s) 40, 80, 180, 204, 316, 390, 422, 471
DpnI GATC 2 cut(s) 48, 441
DpnII GATC 2 cut(s) 46, 439
EaeI YGGCCR 1 cut(s) 78
EagI CGGCCG 1 cut(s) 78
EclXI CGGCCG 1 cut(s) 78
Eco47I GGWCC 2 cut(s) 131, 298
Eco47III AGCGCT 1 cut(s) 329
Eco52I CGGCCG 1 cut(s) 78
Eco57I CTGAAG 1 cut(s) 210
EcoO109I RGGNCCY 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 289
FaeI CATG 1 cut(s) 178
FatI CATG 1 cut(s) 174
Fnu4HI GCNGC 4 cut(s) 6, 423, 472, 475
FokI GGATG 1 cut(s) 225
Fsp4HI GCNGC 4 cut(s) 6, 423, 472, 475
GlaI GCGC 1 cut(s) 329
GluI GCNGC 4 cut(s) 6, 423, 472, 475
GsuI CTGGAG 1 cut(s) 14
HaeII RGCGCY 1 cut(s) 331
HaeIII GGCC 1 cut(s) 80
HapII CCGG 2 cut(s) 62, 81
HgaI GACGC 3 cut(s) 19, 106, 343
HhaI GCGC 1 cut(s) 330
Hin1I GRCGYC 1 cut(s) 335
Hin1II CATG 1 cut(s) 178
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HinfI GANTC 3 cut(s) 19, 72, 401
HpaII CCGG 2 cut(s) 62, 81
HphI GGTGA 2 cut(s) 275, 305
Hpy166II GTNNAC 1 cut(s) 222
Hpy188I TCNGA 5 cut(s) 131, 198, 210, 229, 444
Hpy188III TCNNGA 5 cut(s) 31, 121, 175, 364, 457
Hpy8I GTNNAC 1 cut(s) 222
Hpy99I CGWCG 2 cut(s) 13, 214
HpyCH4III ACNGT 3 cut(s) 214, 349, 432
HpyCH4V TGCA 5 cut(s) 54, 270, 320, 410, 425
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 142, 334
Hsp92I GRCGYC 1 cut(s) 335
Hsp92II CATG 1 cut(s) 178
HspAI GCGC 1 cut(s) 328
Kzo9I GATC 2 cut(s) 46, 439
LmnI GCTCC 1 cut(s) 114
Lsp1109I GCAGC 2 cut(s) 409, 461
LweI GCATC 2 cut(s) 247, 486
MaeIII GTNAC 1 cut(s) 118
MalI GATC 2 cut(s) 48, 441
MboI GATC 2 cut(s) 46, 439
MboII GAAGA 2 cut(s) 14, 272
MflI RGATCY 1 cut(s) 46
MluCI AATT 2 cut(s) 190, 452
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 6 cut(s) 38, 145, 216, 289, 442, 474
MseI TTAA 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 243
MspA1I CMGCKG 1 cut(s) 474
MspI CCGG 2 cut(s) 62, 81
MspR9I CCNGG 2 cut(s) 62, 291
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 14, 142, 334
NciI CCSGG 1 cut(s) 62
NdeII GATC 2 cut(s) 46, 439
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 1 cut(s) 300
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 1 cut(s) 118
PagI TCATGA 1 cut(s) 174
PfeI GAWTC 2 cut(s) 72, 401
PkrI GCNGC 4 cut(s) 7, 424, 473, 476
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PpuMI RGGWCCY 1 cut(s) 298
PshAI GACNNNNGTC 1 cut(s) 338
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 300
PspPI GGNCC 2 cut(s) 131, 298
PspPPI RGGWCCY 1 cut(s) 298
PstNI CAGNNNCTG 1 cut(s) 474
PsuI RGATCY 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 243
Rsr2I CGGWCCG 1 cut(s) 131
RsrII CGGWCCG 1 cut(s) 131
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 4 cut(s) 6, 423, 472, 475
Sau3AI GATC 2 cut(s) 46, 439
Sau96I GGNCC 2 cut(s) 131, 298
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 2 cut(s) 62, 291
SetI ASST 5 cut(s) 42, 182, 318, 424, 468
SfaNI GCATC 2 cut(s) 247, 486
SinI GGWCC 2 cut(s) 131, 298
SmiMI CAYNNNNRTG 1 cut(s) 243
SmlI CTYRAG 2 cut(s) 41, 364
SmoI CTYRAG 2 cut(s) 41, 364
Sse9I AATT 2 cut(s) 190, 452
SsiI CCGC 3 cut(s) 5, 134, 472
StyD4I CCNGG 2 cut(s) 60, 289
TaaI ACNGT 3 cut(s) 214, 349, 432
TaqI TCGA 2 cut(s) 66, 90
TasI AATT 2 cut(s) 190, 452
TauI GCSGC 2 cut(s) 8, 474
TfiI GAWTC 2 cut(s) 72, 401
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TscAI CASTG 1 cut(s) 437
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 2 cut(s) 422, 474
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 2 cut(s) 108, 414
TspRI CASTG 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 131, 298
XapI RAATTY 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.