FvH4_3g40880
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005593
Physical Location & Seq
Forward (+)
34423437 .. 34432018
8582 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40880.t2

Sequence Viewer

Length: 2877 bp
ATGTATCAATCTAGAGCTTGGACATGTGTAGCCATTTTGGCTGTGTGCTTGTGCTGGTCTTCACTACAAGGAGTACAAGCCGCTGCACAAGGGCAGGAGATTACCGACCCGGTAGAAGTGACGGCATTGAAGGCCATAAAGAAAAGTTTGATTGATCCCAACAAGAATCTGAGCAATTGGAACCAAGGGGATCCATGTACAGCAAACTGGACAGGCGTTTTGTGCTTCAACGGGTCATTTGATGATGGATATCTTCATGTTCAAGAATTGCTACTGTTAGGTATGAATCTCTCAGGAACTTTGTCACCAGAGCTTGGCCGCTTATCTTATATGAGAATATTGGATTTTATGTGGAACAAAATAAGTGGGAGTATACCAAAGGAGATAGGCAACATTACATCTTTGGAACTCCTGCTGCTGAATGGAAACCAGTTGTCAGGTCCCCTGCCTGAAGAGCTTGGTTATCTCCCGAACTTGGACAGAATACAAATTGACCAGAACTATATTTCAGGACCAATTCCTAAATCATTTGCGAATTTAGACAAAACAAAGCACTTTCACATGAACAACAATTCAATTAGTGGGCAAATTCCATCTGAGCTTTCCAGATTACCGAGTCTTGTCCACTTTCTTCTTGACAACAACAACTTATCTGGGTATCTTCCACCAGAGTTCTCAGATCTGCCAAACTTGTTAATACTTCAACTTGACAACAACAACTTTGATGGGAGTACAATTCCAGCTTCTTACGGCAACATGTCTAAACTGTTGAAGTTGAGTCTCAGGAACTGCAGCTTACAAGGACCCATTCCTGATTTGAGTAGGATACCCAAACTTGGATTTTTAGATCTAAGTAGGAATCAGCTAAATGGATCCATACCTCTTGGAAAACTTTCTGATGACATCACAACGATAAATTTATCTAACAACACTCTAAGTGGGAGCATTCCTGCCAGCTTTTCTGGTCTTCCACAGCTTCAAAGATTGTCCATTGCAAACAATTCACTGACTGGATCTGTTCCAGCAACCCTTTGGAATGAGACGACACTCAATGCAACAGAAAGACTAATAGTGGAGTTGCAGAACAATCAGCTTACAAATATATCTGGCAGTACCCAAATCCCACAGAATGTCACTGTCTGGCTTCATGGAAATCCAGTTTGCTCGAATGCTAACCTGGACAATTTGTGTGGATCTGAAATTGATGATGAAGATGACAGTGAGAGTTCAACCAACTCTACTGCTGGCTGTCCAAGTCAAGCATGCCCGCCCCCTTTCGAATATCTTCCTGCATTTTGTTTCTGTGCTGTTCCACTGCTTATAGAATATCGGTTGAAAAGTCCTGGATTCACTGATTTTCGCCCTTACAGAATTACATTTGAGGAGTATCTTACATCTGGTCTCAACTTAGATCTTGATCAACTGGACATTCCGTCATTTGTATGGGAAAAGGGACCTCGACTGAGAATATCTCTGAAGCTTTTCCCTGCATATGTCGCTAATAATGATACTATTTCGCGTGAATTCAATAAAAGTGAAGTCCAAAGAATCCTTCACAAGTTCACATCATGGAACATTAATGACAGTGAATTATTTGGACCTTATGAGCTTATTTGGATCACACTATTGGATCCTTACAAGGACGTACTATCCGCCTCTAAGAAGTCTGGTGTAAGCAAGGGTGCTCTGGCTGGCATAATAGTGGGAACTTTTGCTGCTGCGGTTGCATTATCTGCAGTTGTCTCTTTATTTATATTGAGAAGGCATCAGAGGAACCACCCTGCTGTTTCTAGAAGACGTCATAAATCGAAGAGTTCCATAAAAATTGATGGTGTTAAGGCTTTCACTTATGGAGAGATGGCCACTGCTACGAACAATTTTAACACCTCTGCTCAAGTTGGCCAAGGTGGTTATGGAAAGGTTTACAAGGGCACTCTCGCTGATGGCACACTTGTGGCAATAAAACGTGCACAAGAAGGATCATTACAGGGTGAGAAGGAGTTTCTAACAGAAATAGAACTATTGTCACCCTTACATCATAGGAATCTGGTCTCCTTAGTTGGATATTGTGATGATGAAGGTGAACAGATGCTGGTTTATGAATTCATGTCCCATGGCACTCTAAGGGATCACCTCTCTGTTAGGTCGAAAGAACCACTTGGATTTGAAATGAGATTGAGAATTGCTCTGGGATCAGCCAGGGGCATCCTCTACCTACATACAGAAGCCAACCCTCCGATATTCCATCGAGATATCAAGGCCAGCAACATATTATTGGACTCGAAGTTTGTGGCAAAAGTTGCTGATTTTGGACTTTCCCGACTTGCCCCGGTACCTGATCTTGAAGGAGCTACACCTGCTCATGTATCCACAGTAGTGAAGGGGACGCCAGGTTACCTTGATCCAGAGTACTTCTTAACCCATAAGCTAACAGACAAGAGTGACGTTTATAGCCTCGGAGTTGTGTTTCTGGAGCTCTTAACCGGAATGCAGCCAATCTCACATGGAAAAAACATTGTTCGAGAGGTAAATATTGCATTCCAGTCCGGTATGATCTTCTCAGTAATTGATGCCCGAATGGGGTCTTATCCTTCTGACTGTGTGGAAAAGTTCTTGAGTTTAGCCCTCAAGTGTTGTCAAGATGAGACTGATGCAAGACCCTCAATGGCAGAGGTGGTTCGAGAACTTGAAAACATATGGTTTATGATGCCTGAGATGGACAGCAGAACAACGGAATCAGTGCTCAGTAGTAGTACAGGAAAGGTAGTAAGCGATCCACCATCTTCATCCAATGCAGGGAAGAATCCTTATGTTTCTGAAGATGTCTCTGGCAGTGACCTTGTTAGCGGAGTCGTACCTACAATCACACCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

959

Amino Acids

105.11

Weight (kDa)

5.51

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 39 - 75 2.4e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 157 - 217 3.4e-07 Leucine rich repeat
LRR_8 PF13855 303 - 336 5.2e-06 Leucine rich repeat
Pkinase PF00069 628 - 895 8.6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 629 - 897 1.8e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2365
AatII GACGTC 1 cut(s) 1801
Acc36I ACCTGC 1 cut(s) 2365
Acc65I GGTACC 1 cut(s) 2332
AccB1I GGYRCC 1 cut(s) 2332
AccB7I CCANNNNNTGG 1 cut(s) 314
AccI GTMKAC 1 cut(s) 373
AccII CGCG 1 cut(s) 1519
AciI CCGC 6 cut(s) 81, 319, 1268, 1653, 1721, 2847
AcoI YGGCCR 3 cut(s) 316, 1860, 1900
AcsI RAATTY 5 cut(s) 535, 588, 916, 1523, 2102
AcuI CTGAAG 3 cut(s) 471, 1496, 2838
AcyI GRCGYC 2 cut(s) 1798, 2387
AfaI GTAC 9 cut(s) 75, 199, 733, 1114, 1647, 2334, 2411, 2755, 2856
AfiI CCNNNNNNNGG 5 cut(s) 314, 475, 836, 2580, 2796
AflIII ACRYGT 2 cut(s) 23, 756
AhdI GACNNNNNGTC 1 cut(s) 1432
AjnI CCWGG 4 cut(s) 1176, 1342, 2198, 2389
AjuI GAANNNNNNNTTGG 2 cut(s) 1536, 1568
AleI CACNNNNGTG 2 cut(s) 1952, 2375
Alw21I GWGCWC 4 cut(s) 1687, 1972, 2478, 2745
Alw26I GTCTC 7 cut(s) 785, 1034, 1406, 1747, 2056, 2639, 2830
Alw44I GTGCAC 1 cut(s) 1968
AlwNI CAGNNNCTG 2 cut(s) 789, 2092
AoxI GGCC 5 cut(s) 132, 316, 1860, 1900, 2259
ApaLI GTGCAC 1 cut(s) 1968
ApeKI GCWGC 6 cut(s) 83, 415, 792, 1715, 1718, 2491
ApoI RAATTY 5 cut(s) 535, 588, 916, 1523, 2102
ArsI GACNNNNNNTTYG 4 cut(s) 202, 234, 2270, 2302
AseI ATTAAT 1 cut(s) 1578
Asp700I GAANNNNTTC 3 cut(s) 892, 1284, 1481
Asp718I GGTACC 1 cut(s) 2332
AspS9I GGNCC 5 cut(s) 440, 512, 803, 1454, 1598
AsuC2I CCSGG 2 cut(s) 110, 2330
AsuHPI GGTGA 5 cut(s) 297, 2003, 2019, 2093, 2123
AsuII TTCGAA 1 cut(s) 1278
AvaII GGWCC 5 cut(s) 440, 512, 803, 1454, 1598
BaeGI GKGCMC 2 cut(s) 1934, 1972
BalI TGGCCA 2 cut(s) 1862, 1902
BamHI GGATCC 3 cut(s) 190, 872, 1630
BanI GGYRCC 1 cut(s) 2332
BanII GRGCYC 1 cut(s) 2478
BarI GAAGNNNNNNTAC 2 cut(s) 1968, 2000
BbsI GAAGAC 3 cut(s) 51, 959, 1801
Bbv12I GWGCWC 4 cut(s) 1687, 1972, 2478, 2745
BbvI GCAGC 6 cut(s) 70, 402, 804, 1702, 1705, 2503
BccI CCATC 9 cut(s) 239, 601, 719, 1823, 1852, 1937, 2253, 2710, 2788
BceAI ACGGC 2 cut(s) 138, 766
BciT130I CCWGG 4 cut(s) 1178, 1344, 2200, 2391
BciVI GTATCC 2 cut(s) 819, 2377
BclI TGATCA 1 cut(s) 1417
BcnI CCSGG 2 cut(s) 110, 2330
BcoDI GTCTC 7 cut(s) 785, 1034, 1406, 1747, 2056, 2639, 2830
BfaI CTAG 2 cut(s) 12, 1791
BfmI CTRYAG 2 cut(s) 790, 1734
BfuAI ACCTGC 1 cut(s) 2365
BfuI GTATCC 2 cut(s) 819, 2377
BglI GCCNNNNNGGC 1 cut(s) 38
BglII AGATCT 3 cut(s) 679, 847, 1411
BisI GCNGC 8 cut(s) 81, 84, 319, 416, 793, 1716, 1719, 2492
BlsI GCNGC 8 cut(s) 82, 85, 320, 417, 794, 1717, 1720, 2493
BmcAI AGTACT 1 cut(s) 2411
Bme1390I CCNGG 6 cut(s) 110, 1178, 1344, 2200, 2330, 2391
Bme18I GGWCC 5 cut(s) 440, 512, 803, 1454, 1598
BmeRI GACNNNNNGTC 1 cut(s) 1432
BmgT120I GGNCC 5 cut(s) 440, 512, 803, 1454, 1598
BmiI GGNNCC 9 cut(s) 182, 192, 442, 805, 874, 1455, 1632, 1775, 2334
BmrFI CCNGG 6 cut(s) 110, 1178, 1344, 2200, 2330, 2391
BmsI GCATC 6 cut(s) 1774, 2079, 2214, 2560, 2641, 2697
BpiI GAAGAC 3 cut(s) 51, 959, 1801
BplI GAGNNNNNCTC 4 cut(s) 1456, 1488, 2170, 2202
BpmI CTGGAG 1 cut(s) 2492
Bpu14I TTCGAA 1 cut(s) 1278
BpuEI CTTGAG 3 cut(s) 1878, 2612, 2635
BpuMI CCSGG 2 cut(s) 110, 2330
BsaBI GATNNNNATC 2 cut(s) 249, 1416
BsaHI GRCGYC 2 cut(s) 1798, 2387
BsaI GGTCTC 2 cut(s) 1406, 2056
BsaJI CCNNGG 6 cut(s) 184, 1903, 2113, 2199, 2328, 2455
BsaWI WCCGGW 2 cut(s) 2483, 2546
Bsc4I CCNNNNNNNGG 5 cut(s) 314, 475, 836, 2580, 2796
Bse1I ACTGG 6 cut(s) 212, 430, 1015, 1157, 1428, 2542
Bse3DI GCAATG 1 cut(s) 990
Bse8I GATNNNNATC 2 cut(s) 249, 1416
BseBI CCWGG 4 cut(s) 1178, 1344, 2200, 2391
BseDI CCNNGG 6 cut(s) 184, 1903, 2113, 2199, 2328, 2455
BseGI GGATG 2 cut(s) 2205, 2786
BseJI GATNNNNATC 2 cut(s) 249, 1416
BseLI CCNNNNNNNGG 5 cut(s) 314, 475, 836, 2580, 2796
BseMI GCAATG 1 cut(s) 990
BseMII CTCAG 9 cut(s) 161, 306, 588, 690, 796, 1454, 2574, 2703, 2758
BseNI ACTGG 6 cut(s) 212, 430, 1015, 1157, 1428, 2542
BseRI GAGGAG 1 cut(s) 1397
BseSI GKGCMC 2 cut(s) 1934, 1972
BseXI GCAGC 6 cut(s) 70, 402, 804, 1702, 1705, 2503
BsgI GTGCAG 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 1519
BshFI GGCC 5 cut(s) 134, 318, 1862, 1902, 2261
BshNI GGYRCC 1 cut(s) 2332
BsiHKAI GWGCWC 4 cut(s) 1687, 1972, 2478, 2745
BsiSI CCGG 4 cut(s) 110, 2330, 2484, 2547
BslFI GGGAC 4 cut(s) 426, 1467, 2095, 2398
BslI CCNNNNNNNGG 5 cut(s) 314, 475, 836, 2580, 2796
BsmAI GTCTC 7 cut(s) 785, 1034, 1406, 1747, 2056, 2639, 2830
BsmBI CGTCTC 1 cut(s) 1034
BsmFI GGGAC 4 cut(s) 426, 1467, 2095, 2398
BsmI GAATGC 4 cut(s) 945, 1174, 2493, 2537
BsnI GGCC 5 cut(s) 134, 318, 1862, 1902, 2261
Bso31I GGTCTC 2 cut(s) 1406, 2056
Bsp119I TTCGAA 1 cut(s) 1278
Bsp1286I GDGCHC 5 cut(s) 1687, 1934, 1972, 2478, 2745
Bsp1407I TGTACA 1 cut(s) 197
Bsp19I CCATGG 1 cut(s) 2113
BspACI CCGC 6 cut(s) 81, 319, 1268, 1653, 1721, 2847
BspANI GGCC 5 cut(s) 134, 318, 1862, 1902, 2261
BspCNI CTCAG 9 cut(s) 162, 305, 589, 689, 795, 1455, 2573, 2704, 2757
BspFNI CGCG 1 cut(s) 1519
BspLI GGNNCC 9 cut(s) 182, 192, 442, 805, 874, 1455, 1632, 1775, 2334
BspMAI CTGCAG 2 cut(s) 794, 1738
BspMI ACCTGC 1 cut(s) 2365
BspQI GCTCTTC 1 cut(s) 447
BspT104I TTCGAA 1 cut(s) 1278
BspT107I GGYRCC 1 cut(s) 2332
BspTNI GGTCTC 2 cut(s) 1406, 2056
BsrDI GCAATG 1 cut(s) 990
BsrGI TGTACA 1 cut(s) 197
BsrI ACTGG 6 cut(s) 212, 430, 1015, 1157, 1428, 2542
BssECI CCNNGG 6 cut(s) 184, 1903, 2113, 2199, 2328, 2455
BssNAI GTATAC 1 cut(s) 374
BssNI GRCGYC 2 cut(s) 1798, 2387
BssT1I CCWWGG 3 cut(s) 184, 1903, 2113
Bst1107I GTATAC 1 cut(s) 374
Bst2UI CCWGG 4 cut(s) 1178, 1344, 2200, 2391
Bst4CI ACNGT 7 cut(s) 276, 768, 1138, 1220, 1586, 2374, 2600
Bst6I CTCTTC 2 cut(s) 447, 1805
BstACI GRCGYC 2 cut(s) 1798, 2387
BstAPI GCANNNNNTGC 2 cut(s) 1733, 2300
BstAUI TGTACA 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 1278
BstC8I GCNNGC 6 cut(s) 955, 1246, 1264, 1268, 1693, 2263
BstDSI CCRYGG 1 cut(s) 2113
BstEII GGTNACC 1 cut(s) 2393
BstF5I GGATG 2 cut(s) 2205, 2786
BstFNI CGCG 1 cut(s) 1519
BstMAI GTCTC 7 cut(s) 785, 1034, 1406, 1747, 2056, 2639, 2830
BstMWI GCNNNNNNNGC 9 cut(s) 38, 131, 222, 454, 1496, 1724, 1733, 2300, 2357
BstNI CCWGG 4 cut(s) 1178, 1344, 2200, 2391
BstNSI RCATGY 3 cut(s) 27, 760, 1266
BstPI GGTNACC 1 cut(s) 2393
BstSCI CCNGG 6 cut(s) 108, 1176, 1342, 2198, 2328, 2389
BstSFI CTRYAG 2 cut(s) 790, 1734
BstSLI GKGCMC 2 cut(s) 1934, 1972
BstUI CGCG 1 cut(s) 1519
BstV1I GCAGC 6 cut(s) 70, 402, 804, 1702, 1705, 2503
BstV2I GAAGAC 3 cut(s) 51, 959, 1801
BstX2I RGATCY 8 cut(s) 190, 679, 847, 872, 1013, 1193, 1411, 1630
BstYI RGATCY 8 cut(s) 190, 679, 847, 872, 1013, 1193, 1411, 1630
BstZ17I GTATAC 1 cut(s) 374
BsuI GTATCC 2 cut(s) 819, 2377
BsuRI GGCC 5 cut(s) 134, 318, 1862, 1902, 2261
BtgI CCRYGG 1 cut(s) 2113
BtsCI GGATG 2 cut(s) 2205, 2786
BtsI GCAGTG 3 cut(s) 1313, 1863, 2839
BtsIMutI CAGTG 9 cut(s) 1004, 1134, 1225, 1313, 1350, 1591, 1863, 2745, 2839
BveI ACCTGC 1 cut(s) 2365
Cac8I GCNNGC 6 cut(s) 955, 1246, 1264, 1268, 1693, 2263
CaiI CAGNNNCTG 2 cut(s) 789, 2092
Cfr13I GGNCC 5 cut(s) 440, 512, 803, 1454, 1598
CseI GACGC 1 cut(s) 2395
Csp6I GTAC 9 cut(s) 74, 198, 732, 1113, 1646, 2333, 2410, 2754, 2855
CspCI CAANNNNNGTGG 4 cut(s) 346, 381, 1171, 1206
CviQI GTAC 9 cut(s) 74, 198, 732, 1113, 1646, 2333, 2410, 2754, 2855
DriI GACNNNNNGTC 1 cut(s) 1432
EaeI YGGCCR 3 cut(s) 316, 1860, 1900
Eam1104I CTCTTC 2 cut(s) 447, 1805
Eam1105I GACNNNNNGTC 1 cut(s) 1432
EarI CTCTTC 2 cut(s) 447, 1805
EciI GGCGGA 1 cut(s) 1642
Ecl136II GAGCTC 1 cut(s) 2476
Eco130I CCWWGG 3 cut(s) 184, 1903, 2113
Eco24I GRGCYC 1 cut(s) 2478
Eco31I GGTCTC 2 cut(s) 1406, 2056
Eco32I GATATC 2 cut(s) 251, 2254
Eco47I GGWCC 5 cut(s) 440, 512, 803, 1454, 1598
Eco53kI GAGCTC 1 cut(s) 2476
Eco57I CTGAAG 3 cut(s) 471, 1496, 2838
Eco91I GGTNACC 1 cut(s) 2393
EcoICRI GAGCTC 1 cut(s) 2476
EcoO109I RGGNCCY 3 cut(s) 440, 803, 1454
EcoO65I GGTNACC 1 cut(s) 2393
EcoRI GAATTC 2 cut(s) 1523, 2102
EcoRII CCWGG 4 cut(s) 1176, 1342, 2198, 2389
EcoRV GATATC 2 cut(s) 251, 2254
EcoT14I CCWWGG 3 cut(s) 184, 1903, 2113
EcoT38I GRGCYC 1 cut(s) 2478
ErhI CCWWGG 3 cut(s) 184, 1903, 2113
Esp3I CGTCTC 1 cut(s) 1034
FalI AAGNNNNNCTT 2 cut(s) 2142, 2174
FaqI GGGAC 4 cut(s) 426, 1467, 2095, 2398
FauI CCCGC 1 cut(s) 1275
FauNDI CATATG 2 cut(s) 1492, 2696
FbaI TGATCA 1 cut(s) 1417
FblI GTMKAC 1 cut(s) 373
Fnu4HI GCNGC 8 cut(s) 81, 84, 319, 416, 793, 1716, 1719, 2492
FokI GGATG 2 cut(s) 2192, 2773
FriOI GRGCYC 1 cut(s) 2478
Fsp4HI GCNGC 8 cut(s) 81, 84, 319, 416, 793, 1716, 1719, 2492
FspBI CTAG 2 cut(s) 12, 1791
GluI GCNGC 8 cut(s) 81, 84, 319, 416, 793, 1716, 1719, 2492
GsuI CTGGAG 1 cut(s) 2492
HaeIII GGCC 5 cut(s) 134, 318, 1862, 1902, 2261
HapII CCGG 4 cut(s) 110, 2330, 2484, 2547
HgaI GACGC 1 cut(s) 2395
Hin1I GRCGYC 2 cut(s) 1798, 2387
HindIII AAGCTT 1 cut(s) 1478
HpaII CCGG 4 cut(s) 110, 2330, 2484, 2547
HphI GGTGA 5 cut(s) 297, 2003, 2019, 2093, 2123
Hpy166II GTNNAC 6 cut(s) 374, 625, 1563, 1924, 1970, 2084
Hpy8I GTNNAC 6 cut(s) 374, 625, 1563, 1924, 1970, 2084
HpyAV CCTTC 9 cut(s) 124, 1562, 1755, 1970, 1990, 2072, 2339, 2374, 2601
HpyCH4III ACNGT 7 cut(s) 276, 768, 1138, 1220, 1586, 2374, 2600
HpyCH4IV ACGT 4 cut(s) 1644, 1798, 1966, 2445
HpyF10VI GCNNNNNNNGC 9 cut(s) 38, 131, 222, 454, 1496, 1724, 1733, 2300, 2357
HpySE526I ACGT 4 cut(s) 1644, 1798, 1966, 2445
Hsp92I GRCGYC 2 cut(s) 1798, 2387
KpnI GGTACC 1 cut(s) 2336
Ksp22I TGATCA 1 cut(s) 1417
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 3 cut(s) 942, 2348, 2473
Lsp1109I GCAGC 6 cut(s) 70, 402, 804, 1702, 1705, 2503
LweI GCATC 6 cut(s) 1774, 2079, 2214, 2560, 2641, 2697
MaeI CTAG 2 cut(s) 12, 1791
MaeII ACGT 4 cut(s) 1644, 1798, 1966, 2445
MaeIII GTNAC 7 cut(s) 118, 303, 1132, 2025, 2393, 2441, 2834
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 8 cut(s) 190, 679, 847, 872, 1013, 1193, 1411, 1630
MhlI GDGCHC 5 cut(s) 1687, 1934, 1972, 2478, 2745
MlsI TGGCCA 2 cut(s) 1862, 1902
MluNI TGGCCA 2 cut(s) 1862, 1902
MlyI GAGTC 4 cut(s) 625, 787, 2273, 2859
MmeI TCCRAC 1 cut(s) 2041
Mox20I TGGCCA 2 cut(s) 1862, 1902
MroXI GAANNNNTTC 3 cut(s) 892, 1284, 1481
MscI TGGCCA 2 cut(s) 1862, 1902
MseI TTAA 6 cut(s) 695, 1578, 1836, 1881, 2417, 2480
MslI CAYNNNNRTG 4 cut(s) 1441, 1700, 1952, 2375
Msp20I TGGCCA 2 cut(s) 1862, 1902
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 4 cut(s) 110, 2330, 2484, 2547
MspR9I CCNGG 6 cut(s) 110, 1178, 1344, 2200, 2330, 2391
MunI CAATTG 1 cut(s) 175
Mva1269I GAATGC 4 cut(s) 945, 1174, 2493, 2537
MvaI CCWGG 4 cut(s) 1178, 1344, 2200, 2391
MvnI CGCG 1 cut(s) 1519
MwoI GCNNNNNNNGC 9 cut(s) 38, 131, 222, 454, 1496, 1724, 1733, 2300, 2357
NciI CCSGG 2 cut(s) 110, 2330
NcoI CCATGG 1 cut(s) 2113
NdeI CATATG 2 cut(s) 1492, 2696
NlaIV GGNNCC 9 cut(s) 182, 192, 442, 805, 874, 1455, 1632, 1775, 2334
NmuCI GTSAC 6 cut(s) 118, 303, 1132, 2025, 2441, 2834
NspI RCATGY 3 cut(s) 27, 760, 1266
NspV TTCGAA 1 cut(s) 1278
OliI CACNNNNGTG 2 cut(s) 1952, 2375
PaeI GCATGC 1 cut(s) 1266
PaqCI CACCTGC 1 cut(s) 2365
PciI ACATGT 2 cut(s) 23, 756
PciSI GCTCTTC 1 cut(s) 447
PctI GAATGC 4 cut(s) 945, 1174, 2493, 2537
PdmI GAANNNNTTC 3 cut(s) 892, 1284, 1481
PfeI GAWTC 8 cut(s) 166, 286, 859, 1347, 1548, 2044, 2735, 2803
PflMI CCANNNNNTGG 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 1342
PkrI GCNGC 8 cut(s) 82, 85, 320, 417, 794, 1717, 1720, 2493
PleI GAGTC 4 cut(s) 624, 786, 2273, 2858
PpsI GAGTC 4 cut(s) 624, 786, 2273, 2858
PpuMI RGGWCCY 3 cut(s) 440, 803, 1454
PscI ACATGT 2 cut(s) 23, 756
PshBI ATTAAT 1 cut(s) 1578
Psp124BI GAGCTC 1 cut(s) 2478
Psp5II RGGWCCY 3 cut(s) 440, 803, 1454
Psp6I CCWGG 4 cut(s) 1176, 1342, 2198, 2389
PspEI GGTNACC 1 cut(s) 2393
PspGI CCWGG 4 cut(s) 1176, 1342, 2198, 2389
PspN4I GGNNCC 9 cut(s) 182, 192, 442, 805, 874, 1455, 1632, 1775, 2334
PspPI GGNCC 5 cut(s) 440, 512, 803, 1454, 1598
PspPPI RGGWCCY 3 cut(s) 440, 803, 1454
PstI CTGCAG 2 cut(s) 794, 1738
PstNI CAGNNNCTG 2 cut(s) 789, 2092
PsuI RGATCY 8 cut(s) 190, 679, 847, 872, 1013, 1193, 1411, 1630
RsaI GTAC 9 cut(s) 75, 199, 733, 1114, 1647, 2334, 2411, 2755, 2856
RsaNI GTAC 9 cut(s) 74, 198, 732, 1113, 1646, 2333, 2410, 2754, 2855
RseI CAYNNNNRTG 4 cut(s) 1441, 1700, 1952, 2375
SacI GAGCTC 1 cut(s) 2478
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 6 cut(s) 695, 1578, 1836, 1881, 2417, 2480
SatI GCNGC 8 cut(s) 81, 84, 319, 416, 793, 1716, 1719, 2492
Sau96I GGNCC 5 cut(s) 440, 512, 803, 1454, 1598
ScaI AGTACT 1 cut(s) 2411
SchI GAGTC 4 cut(s) 625, 787, 2273, 2859
ScrFI CCNGG 6 cut(s) 110, 1178, 1344, 2200, 2330, 2391
SduI GDGCHC 5 cut(s) 1687, 1934, 1972, 2478, 2745
SfaNI GCATC 6 cut(s) 1774, 2079, 2214, 2560, 2641, 2697
SfcI CTRYAG 2 cut(s) 790, 1734
SfuI TTCGAA 1 cut(s) 1278
SinI GGWCC 5 cut(s) 440, 512, 803, 1454, 1598
SmiMI CAYNNNNRTG 4 cut(s) 1441, 1700, 1952, 2375
SmlI CTYRAG 3 cut(s) 1893, 2614, 2627
SmoI CTYRAG 3 cut(s) 1893, 2614, 2627
SphI GCATGC 1 cut(s) 1266
SsiI CCGC 6 cut(s) 81, 319, 1268, 1653, 1721, 2847
SspI AATATT 2 cut(s) 339, 2533
SspMI CTAG 2 cut(s) 12, 1791
SstI GAGCTC 1 cut(s) 2478
StyD4I CCNGG 6 cut(s) 108, 1176, 1342, 2198, 2328, 2389
StyI CCWWGG 3 cut(s) 184, 1903, 2113
TaaI ACNGT 7 cut(s) 276, 768, 1138, 1220, 1586, 2374, 2600
TaiI ACGT 4 cut(s) 1647, 1801, 1969, 2448
TaqI TCGA 9 cut(s) 1166, 1278, 1459, 1808, 2147, 2248, 2282, 2521, 2680
TatI WGTACW 5 cut(s) 73, 197, 731, 2409, 2753
TauI GCSGC 2 cut(s) 83, 321
TfiI GAWTC 8 cut(s) 166, 286, 859, 1347, 1548, 2044, 2735, 2803
Tru1I TTAA 6 cut(s) 695, 1578, 1836, 1881, 2417, 2480
Tru9I TTAA 6 cut(s) 695, 1578, 1836, 1881, 2417, 2480
TscAI CASTG 9 cut(s) 1011, 1141, 1225, 1320, 1357, 1591, 1870, 2745, 2839
TseFI GTSAC 6 cut(s) 118, 303, 1132, 2025, 2441, 2834
TseI GCWGC 6 cut(s) 83, 415, 792, 1715, 1718, 2491
Tsp45I GTSAC 6 cut(s) 118, 303, 1132, 2025, 2441, 2834
TspDTI ATGAA 9 cut(s) 245, 299, 578, 1136, 1224, 2091, 2095, 2115, 2775
TspGWI ACGGA 2 cut(s) 1422, 2747
TspRI CASTG 9 cut(s) 1011, 1141, 1225, 1320, 1357, 1591, 1870, 2745, 2839
Van91I CCANNNNNTGG 1 cut(s) 314
VneI GTGCAC 1 cut(s) 1968
VpaK11BI GGWCC 5 cut(s) 440, 512, 803, 1454, 1598
VspI ATTAAT 1 cut(s) 1578
XapI RAATTY 5 cut(s) 535, 588, 916, 1523, 2102
XbaI TCTAGA 2 cut(s) 11, 1790
XceI RCATGY 3 cut(s) 27, 760, 1266
XcmI CCANNNNNNNNNTGG 2 cut(s) 1029, 1910
XmiI GTMKAC 1 cut(s) 373
XmnI GAANNNNTTC 3 cut(s) 892, 1284, 1481
XspI CTAG 2 cut(s) 12, 1791
ZraI GACGTC 1 cut(s) 1799
ZrmI AGTACT 1 cut(s) 2411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.