RchiOBHm_Chr5g0073661
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005593
Physical Location & Seq
Forward (+)
79709381 .. 79718169
8789 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34854

Sequence Viewer

Length: 2868 bp
ATGGGTCAATCTAGAGCTTGGACGTGTGTGGCCGTTTTGGCCGTGTGCTTGTGCTGGTCTTCACTACAAGCTGGAGCACAAGAGCAGGAAATTACCAACCCCGTAGAAGTGACGGCATTGAAGGCCATAAAGAAAAGTTTGATTGATCCCAACAAGAATCTGAGCAATTGGAACCAAGGGGATCCATGTACAGCAAACTGGACAGGCGTTTTGTGCTTCAACAGATCATTAGATGATGGATATCTTCATGTTCAAGAATTGCAACTGCTAGGTATGAATCTCTCAGGAACTTTGTCACCAGAGCTTGGCCGCCTATCTTATATGCTAATATTGGATTTTATGTGGAATGAAATAAGTGGAAGTATACCAAAGGAGATAGGCAACATTACATCTTTGGAACTCTTACTCCTGAATGGAAACCAATTGTCAGGTCCCCTCCCTGAAGAGCTTGGATATCTTCCGAACTTGGACAGAATACAAATTGACCAGAACTACATTTCAGGACCAATACCTAAATCATTCGCATATTTGAACAAAACGAAGCACTTTCACATGAACAACAATTCAATTAGCGGGCAACTTCCACCTGAGCTTTCCAGATTACCAAAACTTGTCCACATTCTTCTTGACAACAACAACTTGTCTGGGTATCTTCCACCAGAGTTCTCAGATCTGCCGAGCTTATTAATACTTCAACTTGACAACAACAACTTTGGTGGGAGTACAATTCCAGTTTCTTATGGCAACATGTCTAAACTGTTGAAGTTGAGTCTCAGGAACTGCAGCTTGGAAGGACCTATTCCTGATTTCAGTAGGATACCCAACCTTGGATATCTAGATCTAAGTAGGAATCGACTAAATGGATCCATACCTCTGGGAAAACTTTCGGATGACATCACAACGATAAATTTATCTAACAACACTCTTACTGGGAGCATTCCTGCCAACTTTTCCGGTCTTCCACAGCTTCAAAGATTGTCCATTGCAAACAATTCACTGAGTGGTATTGTTCCAGCAGCCCTTTGGAATGATACGACACTTAATGCAACAGAAAGACTAATCCTGGAGTTGCAGAACAATAAGTTTACAAATATATCTGGCAGTACTCAAACCCCGCAGAATGTCACTGTCTGGCTTCAGGGAAATCCAGTTTGCTCGAATGCTAACCTAGACAATTTATGTGGATCTGAAAATGATGATGAAGATGACAGTGAGAGTTCAACCAACTCGACTGCTAGCTGTCCAAGTCAAGCATGCCCGCCTCCTTTCGAATATCTTCCTGTAGTTTGTTTCTGTGCTGTTCCACTACTTATTGATTATCGGTTGAAAAGTCCTGGATTCTCAGATTTTCGCCCTTACAAAAGTACATATGAGAAGTATCTTACGACTGGTCTCCGCTTAGATCTTGATCAACTGGACATTGCTTCATTTGTATGGCAAAAGGGACCTCGACTGAGAATATCACTGAAGCTTTTCCCTTTATATGTTGCTGATAATACCAATATTTCCCATACTTTCAATAGCAGTGAAGTCAAAAGAATCCTTGGCATGTTCACAGGATGGAATATTCCCGATAGTGAAGTGTTCGGACCTTATGAGCTTCTTGGCATCAATCTATTGGATCCTTATAAGGGCGTAATTGTCCCCTCTAAGAAGTCTGGTGTAAGCAAGGGTGCTCTGGCTGGCATAATAGTAGGAACTTTTGCCGGTGCTGTTGCATTATCTGCAGTTGTCTGTTTTTTAATACTGAGGAGGCATCAGAGGAACCACCCTGCTATTTCAAGAAGACGTCGTAAATCGACGAGTTCCATAAAAATTGATGGTGTTAAGGCTTTCACTTATGGAGAAATGGCCACTGCTACAGACAATTTTAACATTTCTGCTCAAATTGGCCAAGGTGGTTATGGAAAGGTTTACAAAGGCACTCTGGCTGATGGCACACTTGTGGCCATAAAACGTGCACAAGAAGGATCGTTACAGGGTGAGAAGGAGTTTCTAACAGAAATAGAACTATTGTCACCCTTACATCATAGGAATCTGGTCTCCTTAGTTGGATACTGTGATGATGAAGGTGAACAGATGTTGGTTTATGAGTTCATGTCGCATGGCACTCTAAGGGATCACCTCTCTGTTAAGTCGAAAGAACCTCTGGGATTTGAAATGAGATTGAGAATTGCTCTGGCATCAGCCAGGGGCATCCTCTACCTACATACAGAAGCCAACCCTCCGATATTCCATCGAGATATCAAGGCCAGCAACATATTATTGGACTCCAAGTTTGTAGCAAAGGTTGCTGATTTTGGACTTTCCCGACTGGCCCCAGTACCTGATCTTGAAGGGACTACGCCTGCTCATGTATCCACAGTAGTGAAGGGGACGCCAGGTTACCTTGATCCGGAGTACTTCTTAACCCATAAACTAACAGACAAGAGTGATGTTTATAGCCTCGGTGTTGTGTTTCTCGAGCTCTTAACTGGAATGCAGCCAATCTCACATGGAAAAAACATTGTTCGAGAGGTAAATATTGCATTCCAATCCGGTATGATCTTCTCAGTAATTGATGGGCGAATGGGGTCTTACCCTTCTGACTGTGTGGAAAAGTTTTTGAGTTTAGCCCTCAAGTGTTGTCAAGATGAGACAGATGCAAGACCCTCAATGGCAGAGGTGGTTCGAGAACTCGAAAACATATGGTCTATGATGCCGGATACGGACAGCAGAACAACGGAATCAGTGCTCAGTAGTAGTACCGGAAAGGTAGTGAGCGATTCACCATCTTCATCCAATGCAGGGAAGAATCCCTATGTTTCTTCAGATGTCTCCGGCAGTGACCTTGTTAGTGGAGTCATACCTACAATCACACCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

955

Amino Acids

104.46

Weight (kDa)

5.7

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 72 2.2e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 154 - 214 1.6e-06 Leucine rich repeat
LRR_8 PF13855 300 - 342 2.8e-06 Leucine rich repeat
Pkinase PF00069 625 - 891 3.3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 626 - 893 6.3e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1629
AatII GACGTC 1 cut(s) 1792
AccB7I CCANNNNNTGG 1 cut(s) 305
AccI GTMKAC 1 cut(s) 364
AccIII TCCGGA 1 cut(s) 2395
AciI CCGC 5 cut(s) 310, 573, 1115, 1259, 1396
AcoI YGGCCR 6 cut(s) 30, 39, 307, 1851, 1891, 1947
AcsI RAATTY 1 cut(s) 907
AcuI CTGAAG 4 cut(s) 462, 1121, 1487, 2793
AcyI GRCGYC 2 cut(s) 1789, 2378
AdeI CACNNNGTG 1 cut(s) 1001
AfaI GTAC 7 cut(s) 190, 724, 1105, 1366, 2325, 2402, 2746
AfiI CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2395, 2787
AflIII ACRYGT 2 cut(s) 23, 747
AjiI CACGTC 1 cut(s) 24
AjnI CCWGG 4 cut(s) 1062, 1333, 2189, 2380
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AleI CACNNNNGTG 2 cut(s) 1943, 2366
AloI GAACNNNNNNTCC 2 cut(s) 390, 422
Alw21I GWGCWC 5 cut(s) 79, 1678, 1963, 2469, 2736
Alw26I GTCTC 5 cut(s) 776, 1397, 2047, 2630, 2821
Alw44I GTGCAC 1 cut(s) 1959
AlwNI CAGNNNCTG 2 cut(s) 780, 2327
Ama87I CYCGRG 1 cut(s) 2462
Aor13HI TCCGGA 1 cut(s) 2395
AoxI GGCC 9 cut(s) 30, 39, 123, 307, 1851, 1891, 1947, 2250, 2316
ApaLI GTGCAC 1 cut(s) 1959
ApeKI GCWGC 3 cut(s) 783, 1016, 2482
ApoI RAATTY 1 cut(s) 907
ArsI GACNNNNNNTTYG 4 cut(s) 193, 225, 2261, 2293
AseI ATTAAT 1 cut(s) 686
Asp700I GAANNNNTTC 3 cut(s) 883, 1275, 1472
AspS9I GGNCC 6 cut(s) 431, 503, 794, 1445, 1589, 2317
AsuHPI GGTGA 6 cut(s) 288, 1994, 2010, 2084, 2114, 2760
AsuII TTCGAA 1 cut(s) 1269
AsuNHI GCTAGC 1 cut(s) 1235
AvaI CYCGRG 1 cut(s) 2462
AvaII GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
BaeGI GKGCMC 1 cut(s) 1963
BalI TGGCCA 3 cut(s) 1853, 1893, 1949
BamHI GGATCC 3 cut(s) 181, 863, 1621
BanII GRGCYC 1 cut(s) 2469
BarI GAAGNNNNNNTAC 2 cut(s) 1959, 1991
BbsI GAAGAC 3 cut(s) 51, 950, 1792
Bbv12I GWGCWC 5 cut(s) 79, 1678, 1963, 2469, 2736
BbvI GCAGC 3 cut(s) 795, 1028, 2494
BccI CCATC 7 cut(s) 230, 1554, 1814, 1928, 2244, 2555, 2779
BceAI ACGGC 3 cut(s) 17, 26, 129
BciT130I CCWGG 4 cut(s) 1064, 1335, 2191, 2382
BciVI GTATCC 4 cut(s) 810, 2048, 2368, 2698
BclI TGATCA 1 cut(s) 1408
BcoDI GTCTC 5 cut(s) 776, 1397, 2047, 2630, 2821
BfaI CTAG 5 cut(s) 12, 269, 836, 1169, 1236
BfmI CTRYAG 4 cut(s) 781, 1281, 1725, 1860
BfuI GTATCC 4 cut(s) 810, 2048, 2368, 2698
BglI GCCNNNNNGGC 1 cut(s) 38
BglII AGATCT 3 cut(s) 670, 838, 1402
BisI GCNGC 4 cut(s) 310, 784, 1017, 2483
BlsI GCNGC 4 cut(s) 311, 785, 1018, 2484
BmcAI AGTACT 2 cut(s) 1105, 2402
Bme1390I CCNGG 4 cut(s) 1064, 1335, 2191, 2382
Bme18I GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
BmeT110I CYCGRG 1 cut(s) 2462
BmgBI CACGTC 1 cut(s) 24
BmgT120I GGNCC 6 cut(s) 431, 503, 794, 1445, 1589, 2317
BmiI GGNNCC 8 cut(s) 173, 183, 433, 865, 1446, 1623, 1766, 2319
BmrFI CCNGG 4 cut(s) 1064, 1335, 2191, 2382
BmrI ACTGGG 2 cut(s) 939, 2315
BmsI GCATC 6 cut(s) 1617, 1765, 2192, 2205, 2632, 2688
BmtI GCTAGC 1 cut(s) 1239
BmuI ACTGGG 2 cut(s) 939, 2315
BpiI GAAGAC 3 cut(s) 51, 950, 1792
BplI GAGNNNNNCTC 2 cut(s) 2161, 2193
BpmI CTGGAG 2 cut(s) 93, 1085
Bpu10I CCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 1269
BpuEI CTTGAG 1 cut(s) 2603
BsaBI GATNNNNATC 2 cut(s) 240, 1407
BsaHI GRCGYC 2 cut(s) 1789, 2378
BsaI GGTCTC 2 cut(s) 1397, 2047
BsaJI CCNNGG 6 cut(s) 175, 826, 1543, 1894, 2190, 2446
BsaWI WCCGGW 4 cut(s) 953, 2395, 2537, 2747
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2395, 2787
Bse118I RCCGGY 1 cut(s) 1706
Bse1I ACTGG 9 cut(s) 203, 731, 934, 1148, 1393, 1419, 2319, 2321, 2479
Bse3DI GCAATG 2 cut(s) 981, 1419
Bse8I GATNNNNATC 2 cut(s) 240, 1407
BseAI TCCGGA 1 cut(s) 2395
BseBI CCWGG 4 cut(s) 1064, 1335, 2191, 2382
BseDI CCNNGG 6 cut(s) 175, 826, 1543, 1894, 2190, 2446
BseGI GGATG 4 cut(s) 895, 1565, 2196, 2777
BseJI GATNNNNATC 2 cut(s) 240, 1407
BseLI CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2395, 2787
BseMI GCAATG 2 cut(s) 981, 1419
BseNI ACTGG 9 cut(s) 203, 731, 934, 1148, 1393, 1419, 2319, 2321, 2479
BseRI GAGGAG 1 cut(s) 1765
BseSI GKGCMC 1 cut(s) 1963
BseXI GCAGC 3 cut(s) 795, 1028, 2494
BshFI GGCC 9 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2252, 2318
BsiHKAI GWGCWC 5 cut(s) 79, 1678, 1963, 2469, 2736
BsiHKCI CYCGRG 1 cut(s) 2462
BsiSI CCGG 7 cut(s) 954, 1707, 2396, 2538, 2702, 2748, 2820
BslFI GGGAC 5 cut(s) 417, 1458, 1628, 2353, 2389
BslI CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2395, 2787
BsmAI GTCTC 5 cut(s) 776, 1397, 2047, 2630, 2821
BsmFI GGGAC 5 cut(s) 417, 1458, 1628, 2353, 2389
BsmI GAATGC 4 cut(s) 936, 1165, 2484, 2528
BsnI GGCC 9 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2252, 2318
Bso31I GGTCTC 2 cut(s) 1397, 2047
BsoBI CYCGRG 1 cut(s) 2462
Bsp119I TTCGAA 1 cut(s) 1269
Bsp1286I GDGCHC 5 cut(s) 79, 1678, 1963, 2469, 2736
Bsp13I TCCGGA 1 cut(s) 2395
Bsp1407I TGTACA 1 cut(s) 188
BspACI CCGC 5 cut(s) 310, 573, 1115, 1259, 1396
BspANI GGCC 9 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2252, 2318
BspEI TCCGGA 1 cut(s) 2395
BspLI GGNNCC 8 cut(s) 173, 183, 433, 865, 1446, 1623, 1766, 2319
BspMAI CTGCAG 2 cut(s) 785, 1729
BspOI GCTAGC 1 cut(s) 1239
BspQI GCTCTTC 1 cut(s) 438
BspT104I TTCGAA 1 cut(s) 1269
BspTNI GGTCTC 2 cut(s) 1397, 2047
BsrDI GCAATG 2 cut(s) 981, 1419
BsrFI RCCGGY 1 cut(s) 1706
BsrGI TGTACA 1 cut(s) 188
BsrI ACTGG 9 cut(s) 203, 731, 934, 1148, 1393, 1419, 2319, 2321, 2479
BssAI RCCGGY 1 cut(s) 1706
BssECI CCNNGG 6 cut(s) 175, 826, 1543, 1894, 2190, 2446
BssNAI GTATAC 1 cut(s) 365
BssNI GRCGYC 2 cut(s) 1789, 2378
BssT1I CCWWGG 4 cut(s) 175, 826, 1543, 1894
Bst1107I GTATAC 1 cut(s) 365
Bst2UI CCWGG 4 cut(s) 1064, 1335, 2191, 2382
Bst4CI ACNGT 6 cut(s) 759, 1129, 1211, 2060, 2365, 2591
Bst6I CTCTTC 1 cut(s) 438
BstACI GRCGYC 2 cut(s) 1789, 2378
BstAPI GCANNNNNTGC 2 cut(s) 1724, 2291
BstAUI TGTACA 1 cut(s) 188
BstBI TTCGAA 1 cut(s) 1269
BstC8I GCNNGC 7 cut(s) 575, 1237, 1255, 1259, 1684, 2254, 2349
BstEII GGTNACC 1 cut(s) 2384
BstF5I GGATG 4 cut(s) 895, 1565, 2196, 2777
BstMAI GTCTC 5 cut(s) 776, 1397, 2047, 2630, 2821
BstMWI GCNNNNNNNGC 5 cut(s) 38, 122, 213, 1724, 2291
BstNI CCWGG 4 cut(s) 1064, 1335, 2191, 2382
BstNSI RCATGY 3 cut(s) 751, 1257, 1552
BstPI GGTNACC 1 cut(s) 2384
BstSCI CCNGG 4 cut(s) 1062, 1333, 2189, 2380
BstSFI CTRYAG 4 cut(s) 781, 1281, 1725, 1860
BstSLI GKGCMC 1 cut(s) 1963
BstV1I GCAGC 3 cut(s) 795, 1028, 2494
BstV2I GAAGAC 3 cut(s) 51, 950, 1792
BstX2I RGATCY 7 cut(s) 181, 670, 838, 863, 1184, 1402, 1621
BstXI CCANNNNNNTGG 1 cut(s) 874
BstYI RGATCY 7 cut(s) 181, 670, 838, 863, 1184, 1402, 1621
BstZ17I GTATAC 1 cut(s) 365
BsuI GTATCC 4 cut(s) 810, 2048, 2368, 2698
BsuRI GGCC 9 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2252, 2318
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 4 cut(s) 895, 1565, 2196, 2777
BtsI GCAGTG 3 cut(s) 1531, 1854, 2830
BtsIMutI CAGTG 8 cut(s) 995, 1125, 1216, 1463, 1531, 1854, 2736, 2830
Cac8I GCNNGC 7 cut(s) 575, 1237, 1255, 1259, 1684, 2254, 2349
CaiI CAGNNNCTG 2 cut(s) 780, 2327
Cfr10I RCCGGY 1 cut(s) 1706
Cfr13I GGNCC 6 cut(s) 431, 503, 794, 1445, 1589, 2317
CseI GACGC 1 cut(s) 2386
Csp6I GTAC 7 cut(s) 189, 723, 1104, 1365, 2324, 2401, 2745
CspCI CAANNNNNGTGG 4 cut(s) 697, 732, 1162, 1197
CviQI GTAC 7 cut(s) 189, 723, 1104, 1365, 2324, 2401, 2745
DraIII CACNNNGTG 1 cut(s) 1001
EaeI YGGCCR 6 cut(s) 30, 39, 307, 1851, 1891, 1947
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 2467
Eco130I CCWWGG 4 cut(s) 175, 826, 1543, 1894
Eco24I GRGCYC 1 cut(s) 2469
Eco31I GGTCTC 2 cut(s) 1397, 2047
Eco32I GATATC 4 cut(s) 242, 455, 833, 2245
Eco47I GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
Eco53kI GAGCTC 1 cut(s) 2467
Eco57I CTGAAG 4 cut(s) 462, 1121, 1487, 2793
Eco88I CYCGRG 1 cut(s) 2462
Eco91I GGTNACC 1 cut(s) 2384
EcoICRI GAGCTC 1 cut(s) 2467
EcoO109I RGGNCCY 3 cut(s) 431, 794, 1445
EcoO65I GGTNACC 1 cut(s) 2384
EcoRII CCWGG 4 cut(s) 1062, 1333, 2189, 2380
EcoRV GATATC 4 cut(s) 242, 455, 833, 2245
EcoT14I CCWWGG 4 cut(s) 175, 826, 1543, 1894
EcoT38I GRGCYC 1 cut(s) 2469
ErhI CCWWGG 4 cut(s) 175, 826, 1543, 1894
FaqI GGGAC 5 cut(s) 417, 1458, 1628, 2353, 2389
FauI CCCGC 3 cut(s) 566, 1122, 1266
FauNDI CATATG 2 cut(s) 1369, 2687
FbaI TGATCA 1 cut(s) 1408
FblI GTMKAC 1 cut(s) 364
Fnu4HI GCNGC 4 cut(s) 310, 784, 1017, 2483
FokI GGATG 4 cut(s) 902, 1572, 2183, 2764
FriOI GRGCYC 1 cut(s) 2469
Fsp4HI GCNGC 4 cut(s) 310, 784, 1017, 2483
FspBI CTAG 5 cut(s) 12, 269, 836, 1169, 1236
GluI GCNGC 4 cut(s) 310, 784, 1017, 2483
GsuI CTGGAG 2 cut(s) 93, 1085
HaeIII GGCC 9 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2252, 2318
HapII CCGG 7 cut(s) 954, 1707, 2396, 2538, 2702, 2748, 2820
HgaI GACGC 1 cut(s) 2386
Hin1I GRCGYC 2 cut(s) 1789, 2378
HindIII AAGCTT 1 cut(s) 1469
HpaII CCGG 7 cut(s) 954, 1707, 2396, 2538, 2702, 2748, 2820
HphI GGTGA 6 cut(s) 288, 1994, 2010, 2084, 2114, 2760
Hpy166II GTNNAC 7 cut(s) 365, 616, 1086, 1554, 1915, 1961, 2075
Hpy8I GTNNAC 7 cut(s) 365, 616, 1086, 1554, 1915, 1961, 2075
Hpy99I CGWCG 2 cut(s) 1794, 1804
HpyAV CCTTC 8 cut(s) 115, 785, 1961, 1981, 2063, 2330, 2365, 2592
HpyCH4III ACNGT 6 cut(s) 759, 1129, 1211, 2060, 2365, 2591
HpyCH4IV ACGT 3 cut(s) 23, 1789, 1957
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 122, 213, 1724, 2291
HpySE526I ACGT 3 cut(s) 23, 1789, 1957
Hsp92I GRCGYC 2 cut(s) 1789, 2378
Kpn2I TCCGGA 1 cut(s) 2395
Ksp22I TGATCA 1 cut(s) 1408
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 2 cut(s) 74, 933
Lsp1109I GCAGC 3 cut(s) 795, 1028, 2494
LweI GCATC 6 cut(s) 1617, 1765, 2192, 2205, 2632, 2688
MaeI CTAG 5 cut(s) 12, 269, 836, 1169, 1236
MaeII ACGT 3 cut(s) 23, 1789, 1957
MaeIII GTNAC 7 cut(s) 109, 294, 1123, 1974, 2016, 2384, 2825
MfeI CAATTG 2 cut(s) 166, 422
MflI RGATCY 7 cut(s) 181, 670, 838, 863, 1184, 1402, 1621
MhlI GDGCHC 5 cut(s) 79, 1678, 1963, 2469, 2736
MlsI TGGCCA 3 cut(s) 1853, 1893, 1949
MluNI TGGCCA 3 cut(s) 1853, 1893, 1949
MlyI GAGTC 3 cut(s) 778, 2264, 2850
MmeI TCCRAC 1 cut(s) 2032
Mox20I TGGCCA 3 cut(s) 1853, 1893, 1949
MroI TCCGGA 1 cut(s) 2395
MroXI GAANNNNTTC 3 cut(s) 883, 1275, 1472
MscI TGGCCA 3 cut(s) 1853, 1893, 1949
MseI TTAA 8 cut(s) 686, 1041, 1742, 1827, 1872, 2133, 2408, 2471
MslI CAYNNNNRTG 3 cut(s) 1432, 1943, 2366
Msp20I TGGCCA 3 cut(s) 1853, 1893, 1949
MspI CCGG 7 cut(s) 954, 1707, 2396, 2538, 2702, 2748, 2820
MspR9I CCNGG 4 cut(s) 1064, 1335, 2191, 2382
MunI CAATTG 2 cut(s) 166, 422
Mva1269I GAATGC 4 cut(s) 936, 1165, 2484, 2528
MvaI CCWGG 4 cut(s) 1064, 1335, 2191, 2382
MwoI GCNNNNNNNGC 5 cut(s) 38, 122, 213, 1724, 2291
NdeI CATATG 2 cut(s) 1369, 2687
NheI GCTAGC 1 cut(s) 1235
NlaIV GGNNCC 8 cut(s) 173, 183, 433, 865, 1446, 1623, 1766, 2319
NmeAIII GCCGAG 1 cut(s) 702
NmuCI GTSAC 5 cut(s) 109, 294, 1123, 2016, 2825
NspI RCATGY 3 cut(s) 751, 1257, 1552
NspV TTCGAA 1 cut(s) 1269
OliI CACNNNNGTG 2 cut(s) 1943, 2366
PaeI GCATGC 1 cut(s) 1257
PaeR7I CTCGAG 1 cut(s) 2462
PciI ACATGT 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 438
PctI GAATGC 4 cut(s) 936, 1165, 2484, 2528
PdmI GAANNNNTTC 3 cut(s) 883, 1275, 1472
PfeI GAWTC 9 cut(s) 157, 277, 850, 1338, 1539, 2035, 2726, 2765, 2794
PflMI CCANNNNNTGG 1 cut(s) 305
PfoI TCCNGGA 2 cut(s) 1062, 1333
PkrI GCNGC 4 cut(s) 311, 785, 1018, 2484
PleI GAGTC 3 cut(s) 777, 2264, 2849
PpsI GAGTC 3 cut(s) 777, 2264, 2849
PpuMI RGGWCCY 3 cut(s) 431, 794, 1445
PscI ACATGT 1 cut(s) 747
PshBI ATTAAT 1 cut(s) 686
PsiI TTATAA 1 cut(s) 1629
Psp124BI GAGCTC 1 cut(s) 2469
Psp5II RGGWCCY 3 cut(s) 431, 794, 1445
Psp6I CCWGG 4 cut(s) 1062, 1333, 2189, 2380
PspEI GGTNACC 1 cut(s) 2384
PspGI CCWGG 4 cut(s) 1062, 1333, 2189, 2380
PspN4I GGNNCC 8 cut(s) 173, 183, 433, 865, 1446, 1623, 1766, 2319
PspPI GGNCC 6 cut(s) 431, 503, 794, 1445, 1589, 2317
PspPPI RGGWCCY 3 cut(s) 431, 794, 1445
PstI CTGCAG 2 cut(s) 785, 1729
PstNI CAGNNNCTG 2 cut(s) 780, 2327
PsuI RGATCY 7 cut(s) 181, 670, 838, 863, 1184, 1402, 1621
RsaI GTAC 7 cut(s) 190, 724, 1105, 1366, 2325, 2402, 2746
RsaNI GTAC 7 cut(s) 189, 723, 1104, 1365, 2324, 2401, 2745
RseI CAYNNNNRTG 3 cut(s) 1432, 1943, 2366
SacI GAGCTC 1 cut(s) 2469
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 8 cut(s) 686, 1041, 1742, 1827, 1872, 2133, 2408, 2471
SatI GCNGC 4 cut(s) 310, 784, 1017, 2483
Sau96I GGNCC 6 cut(s) 431, 503, 794, 1445, 1589, 2317
ScaI AGTACT 2 cut(s) 1105, 2402
SchI GAGTC 3 cut(s) 778, 2264, 2850
ScrFI CCNGG 4 cut(s) 1064, 1335, 2191, 2382
SduI GDGCHC 5 cut(s) 79, 1678, 1963, 2469, 2736
SfaNI GCATC 6 cut(s) 1617, 1765, 2192, 2205, 2632, 2688
SfcI CTRYAG 4 cut(s) 781, 1281, 1725, 1860
SfiI GGCCNNNNNGGCC 1 cut(s) 38
Sfr274I CTCGAG 1 cut(s) 2462
SfuI TTCGAA 1 cut(s) 1269
SinI GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
SlaI CTCGAG 1 cut(s) 2462
SmiMI CAYNNNNRTG 3 cut(s) 1432, 1943, 2366
SmlI CTYRAG 2 cut(s) 2462, 2618
SmoI CTYRAG 2 cut(s) 2462, 2618
SphI GCATGC 1 cut(s) 1257
SsiI CCGC 5 cut(s) 310, 573, 1115, 1259, 1396
SspI AATATT 4 cut(s) 330, 1504, 1567, 2524
SspMI CTAG 5 cut(s) 12, 269, 836, 1169, 1236
SstI GAGCTC 1 cut(s) 2469
StyD4I CCNGG 4 cut(s) 1062, 1333, 2189, 2380
StyI CCWWGG 4 cut(s) 175, 826, 1543, 1894
TaaI ACNGT 6 cut(s) 759, 1129, 1211, 2060, 2365, 2591
TaiI ACGT 3 cut(s) 26, 1792, 1960
TatI WGTACW 5 cut(s) 188, 722, 1103, 1364, 2400
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 9 cut(s) 157, 277, 850, 1338, 1539, 2035, 2726, 2765, 2794
Tru1I TTAA 8 cut(s) 686, 1041, 1742, 1827, 1872, 2133, 2408, 2471
Tru9I TTAA 8 cut(s) 686, 1041, 1742, 1827, 1872, 2133, 2408, 2471
TscAI CASTG 8 cut(s) 1002, 1132, 1216, 1470, 1531, 1861, 2736, 2830
TseFI GTSAC 5 cut(s) 109, 294, 1123, 2016, 2825
TseI GCWGC 3 cut(s) 783, 1016, 2482
Tsp45I GTSAC 5 cut(s) 109, 294, 1123, 2016, 2825
TspDTI ATGAA 9 cut(s) 236, 290, 363, 569, 1215, 1416, 2082, 2086, 2766
TspGWI ACGGA 2 cut(s) 2723, 2738
TspRI CASTG 8 cut(s) 1002, 1132, 1216, 1470, 1531, 1861, 2736, 2830
Van91I CCANNNNNTGG 1 cut(s) 305
VneI GTGCAC 1 cut(s) 1959
VpaK11BI GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
VspI ATTAAT 1 cut(s) 686
XapI RAATTY 1 cut(s) 907
XbaI TCTAGA 2 cut(s) 11, 835
XceI RCATGY 3 cut(s) 751, 1257, 1552
XcmI CCANNNNNNNNNTGG 2 cut(s) 1020, 1901
XhoI CTCGAG 1 cut(s) 2462
XmiI GTMKAC 1 cut(s) 364
XmnI GAANNNNTTC 3 cut(s) 883, 1275, 1472
XspI CTAG 5 cut(s) 12, 269, 836, 1169, 1236
ZraI GACGTC 1 cut(s) 1790
ZrmI AGTACT 2 cut(s) 1105, 2402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.