RLG00000036402
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005593
Physical Location & Seq
Forward (+)
80049981 .. 80057665
7685 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036402

Sequence Viewer

Length: 2871 bp
ATGTATCAATCTAGAGCTTGGACGTGTGTGGCCGTTTTGGCCGTGTGCTTGTGCTGGTCTTCACTACAAGCTGGAGCACAAGAGCAGGAAATTACCACCCCAGTAGAAGTGATGGCATTGAAGGCCATAAAGGAAAGTTTGATTGATCCCAACAAGAATCTGAGCAATTGGAACCAAGGGGATCCATGTACAGCAAACTGGACAGGCGTTTTGTGCTTTAACAGATCATTAGATGATGGATATCTTCATGTTCAAGAATTGCAACTGCTAGGTATGAATCTCTCAGGAACTTTGTCACCAGAGCTTGGCCGCCTATCTTACATGATAATATTGGATTTTATGTGGAACAACATAAGTGGAAGTATTCCAAAGGAGATAGGCAACATTACATCTTTGGAACTCTTGCTGCTGAATGGAAACCAATTGACAGGTCCCCTGCCTGAAGAGCTTGGTCATCTTCCGAACTTGGACAGAATACAAATTGACCAGAACCAAATTTCAGGACCAATACCTAAATCATTTGCAAATTTGAACAAAACAAAGCACTTTCACATGAACAACAATTCAATTAGCGGGCATATTCCATTTGAGCTTTCCACATTACCGCTTCTTGTCCACTTTCTTCTTGACAACAACAACTTATCTGGGTATCTTCCACCAGAGTTCTCAAATCTGCCGAACTTATTAATACTTCAACTTGACAACAACAACTTTGATGGGAGTACAATTCCGGCTTCTTATGGCAACATGTCTAAGCTGTTGAAGTTGAGTCTCAGGAACTGCAACTTGGAAGGACCTATTCCTGATTTCAGTAGGATACCCAACCTTGGATATTTAGATCTGAGTAGGAATCGGCTAAATGGATCCATACCTCTGGGAAAACTTTCGGATGACATCACAACGATAAATTTATCTAACAACACTCTTACCGGGAGCATTCCTGCCAACTTTTCCGGTCTTCCACATCTTCAAAGATTGTCCCTTGCGAACAATTCACTGAGTGGTATTGTTCCAGCAGCCCTTTGGAATGATACGACACTCAATGCAACAGAAAGACTAATCTTGGAGTTGCAGAACAATCAGCTTACAATTATATCTGGCAGTACTCAAATCCCACAGAATGTCACTGTCAGGCTTCAGGGAAATCCAGTCTGCTCGAATGCTAACCTAGAGAATTTATGCGGATCTGAAAATGATGATGAAGATGACAGTGAGAGTTCAACCAACTCTACCGCTACCTGTCCAAGTCAAGCATGCCCGCCCCCCTTTGAATATCTTCCTGTAGTTTGTTTCTGTGCTGTTCCACTACTTATTGATTATCGGTTGAAAAGTCCTGGATTCTCAGATTTTCGCCCTTACAAAAGTACATACGAAAAATATCTTACATCTGGTCTCCTCTTAGATCTTGATCAACTGGACATTGCTTCATTTTTATGGCAAAAGGGACCTCGACTGAGAATATCACTGAAGCTTTTCCCTATATATGTTGCTGATAATGCCAATATTTCCCGTACTTTCAATAGCAGCGAAGTCCAAAGAATCCTTGGCATGTTCACAGCATGGAAAATTCCCGATAGTGAATTGTTTGGACCTTATGAGCTTCTTGGCATCACTCTATTGGATCCTTATAAGGGCGTAATTGTCCTCTCTAACAAGTCTGGTGTAAGCAAGGGTGCTCTGGCTGGCATAATAGTAGGAACTTTTGCTGGTGCTGTTGCATTATCTGCAGTTGTCTGTTTTTTAATACTGAGAAGGCATCAGAGGAACCACCCTGCTATTTCAAGAAGACGTCGTAAATCGACAAGTTCCATAAAAATTGATGGTGTTAAGGCTTTCACTTACGGAGAAATGGCCACTGCTACAGACAATTTTAACATTTCTGCTCAAATTGGCCAAGGTGGTTATGGAAAGGTTTACAAAGGCACTCTGGCTGATGGCACACTTGTGGCCATAAAACGTGCACAAGAAGGATCGTTACAGGGTGAGAAGGAGTTTCTAACAGAAATAGAACTATTGTCACCCTTACATCATAGGAATCTGGTATCCTTAGTTGGATATTGTGATGATGAAGGTGAACAGATGTTGGTTTATGAGTTCATGTCGCATGGCACTCTAAGGGATCACCTCTCTGCAATTAGGTCGAAAGAACCTCTGGGATTTGAAATGAGATTGAGAATTGCTCTGGCATCAGCCAGGGGCATCCTCTACCTACATACAGAAGCCAACCCTCCGATATTCCATCGAGATATCAAGGCCAGCAACATATTATTGGACTCCAAGTTTGTAGCAAAGGTTGCTGATTTTGGACTTTCCCGACTTGCCCCAGTACCTGATCTTGAAGGGACTACGCCTGCTCATGTATCCACAGTAGTGAAGGGGACGCCAGGTTACCTTGATCCGGAGTACTTCTTAACCCATAAACTAACAGACAAGAGTGATGTTTATAGCCTCGGTGTTGTGTTTCTCGAGCTCTTAACTGGAATGCAGCCAATCTCACATGGAAAAAACATTGTTCGAGAGGTAAATATTGCATTCCAATCCGGTATGATCTTCTCAGTAATTGATGGGCGAATGGGGTCTTATCCTTCTGACTGTGTGGAAAAGTTCTTGAGTTTAGCCCTCAAGTGTTGTCAAGATGAGACAGATGCAAGACCCTCAATGGCAGAGGTGGTTCGAGAACTTGAAAACATATGGTCTATGATGCCGGAGACTGACAGCAGAACAACGGAATCATTGCTCAGTAGTAGTACTGGAAAGGTAGTGAGCGATTCACCATCTTCATCCAATGCAGGGAAGAATCCCTATGTTTCTTCAGATGTCTCCGGCAGTGACCTTGTTAGTGGAGTCATACCTACAATCACACCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

957

Amino Acids

104.68

Weight (kDa)

5.55

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 72 2.5e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 300 - 343 4.2e-07 Leucine rich repeat
Pkinase PF00069 625 - 892 4.2e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 626 - 894 1.7e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1629
AatII GACGTC 1 cut(s) 1792
AccB7I CCANNNNNTGG 1 cut(s) 305
AccIII TCCGGA 1 cut(s) 2398
AciI CCGC 6 cut(s) 310, 573, 605, 1182, 1233, 1259
AcoI YGGCCR 6 cut(s) 30, 39, 307, 1851, 1891, 1947
AcsI RAATTY 5 cut(s) 495, 526, 907, 1174, 1566
AcuI CTGAAG 4 cut(s) 462, 1121, 1487, 2796
AcyI GRCGYC 2 cut(s) 1789, 2381
AdeI CACNNNGTG 1 cut(s) 1001
AfaI GTAC 8 cut(s) 190, 724, 1105, 1366, 1513, 2328, 2405, 2749
AfiI CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2398, 2790
AflIII ACRYGT 2 cut(s) 23, 747
AjiI CACGTC 1 cut(s) 24
AjnI CCWGG 3 cut(s) 1333, 2192, 2383
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AleI CACNNNNGTG 2 cut(s) 1943, 2369
Alw21I GWGCWC 4 cut(s) 79, 1678, 1963, 2472
Alw26I GTCTC 5 cut(s) 776, 1397, 2633, 2702, 2824
Alw44I GTGCAC 1 cut(s) 1959
AlwNI CAGNNNCTG 2 cut(s) 780, 2330
Ama87I CYCGRG 1 cut(s) 2465
Aor13HI TCCGGA 1 cut(s) 2398
AoxI GGCC 8 cut(s) 30, 39, 123, 307, 1851, 1891, 1947, 2253
ApaLI GTGCAC 1 cut(s) 1959
ApeKI GCWGC 4 cut(s) 406, 1016, 1524, 2485
ApoI RAATTY 5 cut(s) 495, 526, 907, 1174, 1566
ArsI GACNNNNNNTTYG 4 cut(s) 193, 225, 2264, 2296
AseI ATTAAT 1 cut(s) 686
Asp700I GAANNNNTTC 3 cut(s) 883, 1275, 1472
AspS9I GGNCC 5 cut(s) 431, 503, 794, 1445, 1589
AsuC2I CCSGG 1 cut(s) 931
AsuHPI GGTGA 6 cut(s) 288, 1994, 2010, 2084, 2114, 2763
AvaI CYCGRG 1 cut(s) 2465
AvaII GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
BaeGI GKGCMC 1 cut(s) 1963
BalI TGGCCA 3 cut(s) 1853, 1893, 1949
BamHI GGATCC 3 cut(s) 181, 863, 1621
BanII GRGCYC 1 cut(s) 2472
BarI GAAGNNNNNNTAC 2 cut(s) 1959, 1991
BbsI GAAGAC 3 cut(s) 51, 950, 1792
Bbv12I GWGCWC 4 cut(s) 79, 1678, 1963, 2472
BbvI GCAGC 4 cut(s) 393, 1028, 1536, 2497
BccI CCATC 8 cut(s) 106, 230, 710, 1814, 1928, 2247, 2558, 2782
BceAI ACGGC 2 cut(s) 17, 26
BcgI CGANNNNNNTGC 2 cut(s) 2121, 2155
BciT130I CCWGG 3 cut(s) 1335, 2194, 2385
BciVI GTATCC 3 cut(s) 810, 2053, 2371
BclI TGATCA 1 cut(s) 1408
BcnI CCSGG 1 cut(s) 931
BcoDI GTCTC 5 cut(s) 776, 1397, 2633, 2702, 2824
BfaI CTAG 3 cut(s) 12, 269, 1169
BfmI CTRYAG 3 cut(s) 1281, 1725, 1860
BfuI GTATCC 3 cut(s) 810, 2053, 2371
BglI GCCNNNNNGGC 1 cut(s) 38
BglII AGATCT 2 cut(s) 838, 1402
BisI GCNGC 5 cut(s) 310, 407, 1017, 1525, 2486
BlsI GCNGC 5 cut(s) 311, 408, 1018, 1526, 2487
BmcAI AGTACT 3 cut(s) 1105, 2405, 2749
Bme1390I CCNGG 4 cut(s) 931, 1335, 2194, 2385
Bme18I GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
BmeT110I CYCGRG 1 cut(s) 2465
BmgBI CACGTC 1 cut(s) 24
BmgT120I GGNCC 5 cut(s) 431, 503, 794, 1445, 1589
BmiI GGNNCC 7 cut(s) 173, 183, 433, 865, 1446, 1623, 1766
BmrFI CCNGG 4 cut(s) 931, 1335, 2194, 2385
BmrI ACTGGG 2 cut(s) 95, 2318
BmsI GCATC 6 cut(s) 1617, 1765, 2195, 2208, 2635, 2691
BmuI ACTGGG 2 cut(s) 95, 2318
BpiI GAAGAC 3 cut(s) 51, 950, 1792
BplI GAGNNNNNCTC 2 cut(s) 2164, 2196
BpmI CTGGAG 1 cut(s) 93
BpuEI CTTGAG 2 cut(s) 2606, 2629
BpuMI CCSGG 1 cut(s) 931
BsaBI GATNNNNATC 2 cut(s) 240, 1407
BsaHI GRCGYC 2 cut(s) 1789, 2381
BsaI GGTCTC 1 cut(s) 1397
BsaJI CCNNGG 6 cut(s) 175, 826, 1543, 1894, 2193, 2449
BsaWI WCCGGW 3 cut(s) 953, 2398, 2540
Bsc4I CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2398, 2790
Bse1I ACTGG 7 cut(s) 101, 203, 1148, 1419, 2324, 2482, 2755
Bse3DI GCAATG 2 cut(s) 1419, 2732
Bse8I GATNNNNATC 2 cut(s) 240, 1407
BseAI TCCGGA 1 cut(s) 2398
BseBI CCWGG 3 cut(s) 1335, 2194, 2385
BseDI CCNNGG 6 cut(s) 175, 826, 1543, 1894, 2193, 2449
BseGI GGATG 3 cut(s) 895, 2199, 2780
BseJI GATNNNNATC 2 cut(s) 240, 1407
BseLI CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2398, 2790
BseMI GCAATG 2 cut(s) 1419, 2732
BseNI ACTGG 7 cut(s) 101, 203, 1148, 1419, 2324, 2482, 2755
BseRI GAGGAG 1 cut(s) 1385
BseSI GKGCMC 1 cut(s) 1963
BseXI GCAGC 4 cut(s) 393, 1028, 1536, 2497
BshFI GGCC 8 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2255
BsiHKAI GWGCWC 4 cut(s) 79, 1678, 1963, 2472
BsiHKCI CYCGRG 1 cut(s) 2465
BsiSI CCGG 7 cut(s) 731, 930, 954, 2399, 2541, 2705, 2823
BslFI GGGAC 5 cut(s) 417, 964, 1458, 2356, 2392
BslI CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2398, 2790
BsmAI GTCTC 5 cut(s) 776, 1397, 2633, 2702, 2824
BsmFI GGGAC 5 cut(s) 417, 964, 1458, 2356, 2392
BsmI GAATGC 4 cut(s) 936, 1165, 2487, 2531
BsnI GGCC 8 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2255
Bso31I GGTCTC 1 cut(s) 1397
BsoBI CYCGRG 1 cut(s) 2465
Bsp1286I GDGCHC 4 cut(s) 79, 1678, 1963, 2472
Bsp13I TCCGGA 1 cut(s) 2398
Bsp1407I TGTACA 1 cut(s) 188
BspACI CCGC 6 cut(s) 310, 573, 605, 1182, 1233, 1259
BspANI GGCC 8 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2255
BspEI TCCGGA 1 cut(s) 2398
BspLI GGNNCC 7 cut(s) 173, 183, 433, 865, 1446, 1623, 1766
BspMAI CTGCAG 1 cut(s) 1729
BspQI GCTCTTC 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 1397
BsrDI GCAATG 2 cut(s) 1419, 2732
BsrGI TGTACA 1 cut(s) 188
BsrI ACTGG 7 cut(s) 101, 203, 1148, 1419, 2324, 2482, 2755
BssECI CCNNGG 6 cut(s) 175, 826, 1543, 1894, 2193, 2449
BssNI GRCGYC 2 cut(s) 1789, 2381
BssT1I CCWWGG 4 cut(s) 175, 826, 1543, 1894
Bst2UI CCWGG 3 cut(s) 1335, 2194, 2385
Bst4CI ACNGT 4 cut(s) 1129, 1211, 2368, 2594
Bst6I CTCTTC 1 cut(s) 438
BstACI GRCGYC 2 cut(s) 1789, 2381
BstAPI GCANNNNNTGC 2 cut(s) 1724, 2294
BstAUI TGTACA 1 cut(s) 188
BstC8I GCNNGC 6 cut(s) 575, 1255, 1259, 1684, 2257, 2352
BstEII GGTNACC 1 cut(s) 2387
BstF5I GGATG 3 cut(s) 895, 2199, 2780
BstMAI GTCTC 5 cut(s) 776, 1397, 2633, 2702, 2824
BstMWI GCNNNNNNNGC 7 cut(s) 38, 122, 213, 445, 1496, 1724, 2294
BstNI CCWGG 3 cut(s) 1335, 2194, 2385
BstNSI RCATGY 3 cut(s) 751, 1257, 1552
BstPI GGTNACC 1 cut(s) 2387
BstSCI CCNGG 4 cut(s) 929, 1333, 2192, 2383
BstSFI CTRYAG 3 cut(s) 1281, 1725, 1860
BstSLI GKGCMC 1 cut(s) 1963
BstV1I GCAGC 4 cut(s) 393, 1028, 1536, 2497
BstV2I GAAGAC 3 cut(s) 51, 950, 1792
BstX2I RGATCY 6 cut(s) 181, 838, 863, 1184, 1402, 1621
BstXI CCANNNNNNTGG 1 cut(s) 874
BstYI RGATCY 6 cut(s) 181, 838, 863, 1184, 1402, 1621
BsuI GTATCC 3 cut(s) 810, 2053, 2371
BsuRI GGCC 8 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2255
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 3 cut(s) 895, 2199, 2780
BtsI GCAGTG 2 cut(s) 1854, 2833
BtsIMutI CAGTG 6 cut(s) 995, 1125, 1216, 1463, 1854, 2833
Cac8I GCNNGC 6 cut(s) 575, 1255, 1259, 1684, 2257, 2352
CaiI CAGNNNCTG 2 cut(s) 780, 2330
Cfr13I GGNCC 5 cut(s) 431, 503, 794, 1445, 1589
CseI GACGC 1 cut(s) 2389
Csp6I GTAC 8 cut(s) 189, 723, 1104, 1365, 1512, 2327, 2404, 2748
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CviQI GTAC 8 cut(s) 189, 723, 1104, 1365, 1512, 2327, 2404, 2748
DraIII CACNNNGTG 1 cut(s) 1001
EaeI YGGCCR 6 cut(s) 30, 39, 307, 1851, 1891, 1947
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 2470
Eco130I CCWWGG 4 cut(s) 175, 826, 1543, 1894
Eco24I GRGCYC 1 cut(s) 2472
Eco31I GGTCTC 1 cut(s) 1397
Eco32I GATATC 2 cut(s) 242, 2248
Eco47I GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
Eco53kI GAGCTC 1 cut(s) 2470
Eco57I CTGAAG 4 cut(s) 462, 1121, 1487, 2796
Eco88I CYCGRG 1 cut(s) 2465
Eco91I GGTNACC 1 cut(s) 2387
EcoICRI GAGCTC 1 cut(s) 2470
EcoO109I RGGNCCY 3 cut(s) 431, 794, 1445
EcoO65I GGTNACC 1 cut(s) 2387
EcoRII CCWGG 3 cut(s) 1333, 2192, 2383
EcoRV GATATC 2 cut(s) 242, 2248
EcoT14I CCWWGG 4 cut(s) 175, 826, 1543, 1894
EcoT38I GRGCYC 1 cut(s) 2472
ErhI CCWWGG 4 cut(s) 175, 826, 1543, 1894
FaqI GGGAC 5 cut(s) 417, 964, 1458, 2356, 2392
FauI CCCGC 2 cut(s) 566, 1266
FauNDI CATATG 1 cut(s) 2690
FbaI TGATCA 1 cut(s) 1408
Fnu4HI GCNGC 5 cut(s) 310, 407, 1017, 1525, 2486
FokI GGATG 3 cut(s) 902, 2186, 2767
FriOI GRGCYC 1 cut(s) 2472
Fsp4HI GCNGC 5 cut(s) 310, 407, 1017, 1525, 2486
FspBI CTAG 3 cut(s) 12, 269, 1169
GluI GCNGC 5 cut(s) 310, 407, 1017, 1525, 2486
GsuI CTGGAG 1 cut(s) 93
HaeIII GGCC 8 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2255
HapII CCGG 7 cut(s) 731, 930, 954, 2399, 2541, 2705, 2823
HgaI GACGC 1 cut(s) 2389
Hin1I GRCGYC 2 cut(s) 1789, 2381
HindIII AAGCTT 1 cut(s) 1469
HpaII CCGG 7 cut(s) 731, 930, 954, 2399, 2541, 2705, 2823
HphI GGTGA 6 cut(s) 288, 1994, 2010, 2084, 2114, 2763
Hpy166II GTNNAC 5 cut(s) 616, 1554, 1915, 1961, 2075
Hpy8I GTNNAC 5 cut(s) 616, 1554, 1915, 1961, 2075
Hpy99I CGWCG 1 cut(s) 1794
HpyAV CCTTC 9 cut(s) 115, 785, 1746, 1961, 1981, 2063, 2333, 2368, 2595
HpyCH4III ACNGT 4 cut(s) 1129, 1211, 2368, 2594
HpyCH4IV ACGT 3 cut(s) 23, 1789, 1957
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 122, 213, 445, 1496, 1724, 2294
HpySE526I ACGT 3 cut(s) 23, 1789, 1957
Hsp92I GRCGYC 2 cut(s) 1789, 2381
Kpn2I TCCGGA 1 cut(s) 2398
Ksp22I TGATCA 1 cut(s) 1408
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 2 cut(s) 74, 933
Lsp1109I GCAGC 4 cut(s) 393, 1028, 1536, 2497
LweI GCATC 6 cut(s) 1617, 1765, 2195, 2208, 2635, 2691
MaeI CTAG 3 cut(s) 12, 269, 1169
MaeII ACGT 3 cut(s) 23, 1789, 1957
MaeIII GTNAC 6 cut(s) 294, 1123, 1974, 2016, 2387, 2828
MfeI CAATTG 2 cut(s) 166, 422
MflI RGATCY 6 cut(s) 181, 838, 863, 1184, 1402, 1621
MhlI GDGCHC 4 cut(s) 79, 1678, 1963, 2472
MlsI TGGCCA 3 cut(s) 1853, 1893, 1949
MluNI TGGCCA 3 cut(s) 1853, 1893, 1949
MlyI GAGTC 3 cut(s) 778, 2267, 2853
MmeI TCCRAC 1 cut(s) 2032
Mox20I TGGCCA 3 cut(s) 1853, 1893, 1949
MroI TCCGGA 1 cut(s) 2398
MroXI GAANNNNTTC 3 cut(s) 883, 1275, 1472
MscI TGGCCA 3 cut(s) 1853, 1893, 1949
MseI TTAA 7 cut(s) 219, 686, 1742, 1827, 1872, 2411, 2474
MslI CAYNNNNRTG 3 cut(s) 1432, 1943, 2369
Msp20I TGGCCA 3 cut(s) 1853, 1893, 1949
MspI CCGG 7 cut(s) 731, 930, 954, 2399, 2541, 2705, 2823
MspR9I CCNGG 4 cut(s) 931, 1335, 2194, 2385
MunI CAATTG 2 cut(s) 166, 422
Mva1269I GAATGC 4 cut(s) 936, 1165, 2487, 2531
MvaI CCWGG 3 cut(s) 1335, 2194, 2385
MwoI GCNNNNNNNGC 7 cut(s) 38, 122, 213, 445, 1496, 1724, 2294
NciI CCSGG 1 cut(s) 931
NdeI CATATG 1 cut(s) 2690
NlaIV GGNNCC 7 cut(s) 173, 183, 433, 865, 1446, 1623, 1766
NmuCI GTSAC 4 cut(s) 294, 1123, 2016, 2828
NspI RCATGY 3 cut(s) 751, 1257, 1552
OliI CACNNNNGTG 2 cut(s) 1943, 2369
PaeI GCATGC 1 cut(s) 1257
PaeR7I CTCGAG 1 cut(s) 2465
PciI ACATGT 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 438
PctI GAATGC 4 cut(s) 936, 1165, 2487, 2531
PdmI GAANNNNTTC 3 cut(s) 883, 1275, 1472
PfeI GAWTC 9 cut(s) 157, 277, 850, 1338, 1539, 2035, 2729, 2768, 2797
PflMI CCANNNNNTGG 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 1333
PkrI GCNGC 5 cut(s) 311, 408, 1018, 1526, 2487
PleI GAGTC 3 cut(s) 777, 2267, 2852
PpsI GAGTC 3 cut(s) 777, 2267, 2852
PpuMI RGGWCCY 3 cut(s) 431, 794, 1445
PscI ACATGT 1 cut(s) 747
PshBI ATTAAT 1 cut(s) 686
PsiI TTATAA 1 cut(s) 1629
Psp124BI GAGCTC 1 cut(s) 2472
Psp5II RGGWCCY 3 cut(s) 431, 794, 1445
Psp6I CCWGG 3 cut(s) 1333, 2192, 2383
PspEI GGTNACC 1 cut(s) 2387
PspGI CCWGG 3 cut(s) 1333, 2192, 2383
PspN4I GGNNCC 7 cut(s) 173, 183, 433, 865, 1446, 1623, 1766
PspPI GGNCC 5 cut(s) 431, 503, 794, 1445, 1589
PspPPI RGGWCCY 3 cut(s) 431, 794, 1445
PstI CTGCAG 1 cut(s) 1729
PstNI CAGNNNCTG 2 cut(s) 780, 2330
PsuI RGATCY 6 cut(s) 181, 838, 863, 1184, 1402, 1621
RsaI GTAC 8 cut(s) 190, 724, 1105, 1366, 1513, 2328, 2405, 2749
RsaNI GTAC 8 cut(s) 189, 723, 1104, 1365, 1512, 2327, 2404, 2748
RseI CAYNNNNRTG 3 cut(s) 1432, 1943, 2369
SacI GAGCTC 1 cut(s) 2472
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 7 cut(s) 219, 686, 1742, 1827, 1872, 2411, 2474
SatI GCNGC 5 cut(s) 310, 407, 1017, 1525, 2486
Sau96I GGNCC 5 cut(s) 431, 503, 794, 1445, 1589
ScaI AGTACT 3 cut(s) 1105, 2405, 2749
SchI GAGTC 3 cut(s) 778, 2267, 2853
ScrFI CCNGG 4 cut(s) 931, 1335, 2194, 2385
SduI GDGCHC 4 cut(s) 79, 1678, 1963, 2472
SfaNI GCATC 6 cut(s) 1617, 1765, 2195, 2208, 2635, 2691
SfcI CTRYAG 3 cut(s) 1281, 1725, 1860
SfiI GGCCNNNNNGGCC 1 cut(s) 38
Sfr274I CTCGAG 1 cut(s) 2465
SinI GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
SlaI CTCGAG 1 cut(s) 2465
SmiMI CAYNNNNRTG 3 cut(s) 1432, 1943, 2369
SmlI CTYRAG 3 cut(s) 2465, 2608, 2621
SmoI CTYRAG 3 cut(s) 2465, 2608, 2621
SphI GCATGC 1 cut(s) 1257
SsiI CCGC 6 cut(s) 310, 573, 605, 1182, 1233, 1259
SspI AATATT 3 cut(s) 330, 1504, 2527
SspMI CTAG 3 cut(s) 12, 269, 1169
SstI GAGCTC 1 cut(s) 2472
StyD4I CCNGG 4 cut(s) 929, 1333, 2192, 2383
StyI CCWWGG 4 cut(s) 175, 826, 1543, 1894
TaaI ACNGT 4 cut(s) 1129, 1211, 2368, 2594
TaiI ACGT 3 cut(s) 26, 1792, 1960
TaqI TCGA 8 cut(s) 1157, 1450, 1799, 2141, 2242, 2466, 2515, 2674
TatI WGTACW 6 cut(s) 188, 722, 1103, 1364, 2403, 2747
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 9 cut(s) 157, 277, 850, 1338, 1539, 2035, 2729, 2768, 2797
Tru1I TTAA 7 cut(s) 219, 686, 1742, 1827, 1872, 2411, 2474
Tru9I TTAA 7 cut(s) 219, 686, 1742, 1827, 1872, 2411, 2474
TscAI CASTG 6 cut(s) 1002, 1132, 1216, 1470, 1861, 2833
TseFI GTSAC 4 cut(s) 294, 1123, 2016, 2828
TseI GCWGC 4 cut(s) 406, 1016, 1524, 2485
Tsp45I GTSAC 4 cut(s) 294, 1123, 2016, 2828
TspDTI ATGAA 8 cut(s) 236, 290, 569, 1215, 1416, 2082, 2086, 2769
TspGWI ACGGA 2 cut(s) 1857, 2741
TspRI CASTG 6 cut(s) 1002, 1132, 1216, 1470, 1861, 2833
Van91I CCANNNNNTGG 1 cut(s) 305
VneI GTGCAC 1 cut(s) 1959
VpaK11BI GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
VspI ATTAAT 1 cut(s) 686
XapI RAATTY 5 cut(s) 495, 526, 907, 1174, 1566
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 3 cut(s) 751, 1257, 1552
XcmI CCANNNNNNNNNTGG 3 cut(s) 1020, 1541, 1901
XhoI CTCGAG 1 cut(s) 2465
XmnI GAANNNNTTC 3 cut(s) 883, 1275, 1472
XspI CTAG 3 cut(s) 12, 269, 1169
ZraI GACGTC 1 cut(s) 1790
ZrmI AGTACT 3 cut(s) 1105, 2405, 2749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.