MD11G1051300.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005593
Physical Location & Seq
Reverse (-)
4385942 .. 4393940
7999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1051300.v1.1.491

Sequence Viewer

Length: 2862 bp
ATGTATCCATCAACAGTTTGGACATATGCATGGGTTTTCGGCATGTTCTTGTGCTGGTCTTCGCTACGAGCTGGAGCACAAGATCTTGGTATCACCCACCCTGCAGAAGTGACGGCGCTGCGGGCCATCAAGAGAAGTTTGGTTGATCCCAACAAGAATCTGAGCAACTGGAATCGAGGAGACCCATGTACGGCAAACTGGACAGGAGTGCTTTGCTACAACAAAACATTAGATGATGGCTATCATCATGTTCAAGAATTGCAACTACTAACTATGAATCTGTTAGGAAGTTTGTCACCAGAGCTTGGCCGCTTATCTAAAATGAAAATATTGGATTTTATGTGGAACAACATAAGTGGAAGTATACCGAAGGAGATAGGCAACATAACAACCTTGGAACTTTTGCTCCTCAACGGAAACCAATTATCAGGTTCCTTGCCTGAAGAGCTCGGTTATCTTCCAAACTTGGACAGAATACAAATCGACCAGAACCAAATATCAGGACCATTACCTAAATCGTTTGCAAATTTGAACAAGACCAAGCATTTTCACATGAACAACAATTCGATTAGCGGGCAAATTCCACCTGAGCTTTCCAGATTACCGAGTCTTGTCCACTTTCTTCTTGACAACAACAACTTATCTGGGTATCTTCCGCCAGAGTTCTCAAAGTTGCCAAACTTATTAATACTTCAAGTTGACAACAACAACTTTGATGGGACTACAATTCCAGATTCGTATAGCAACATGTCTAAGCTGTTGAAGTTGAGTTTGAGGAACTGCAACTTACAAGGGCCTATTCCTGATTTGAGTAGGATTCCAAACCTTGGTTATGTAGACCTAAGCTCAAATCAGCTAAATGGATCCTTGCCTCTGGGTAAACTCTCTGATCAAATCACGACGATCAATTTATCTAACAACACTCTGACCGGAAGAATTCCTGGCAACTTTTCGGAACTTCCTAATCTTCAGAGATTGTCTATAGCAAACAATTCATTGAGTGGCTCCATTCCAGCAGCACTTTGGCAAAACAGGAAACTCAACGCGACAGAAAAACTAATCGTGGAGTTGCAGAACAATGACCTTACAAGTGTAAACGGCAGTACCGCAGTTCCGCAAAATGTCACTGTCGGGCTTCGGGGAAATCCGCTTTGCTCAAATTCCAGTCTTGATAACTTCTGTGGGACCGAAAGTGGTGACAAAAATGACAGTCAGATTTCGATTAACTCCACTGCCAACTGTCCGAGTCAAGCATGCCCACCCCCTTTCGAATATATTCCTGAAGCTTGTTTTTGTGGTGCTCCTCTGCTTGTTGAGTATCGGTTGAAAAGTCCCGGATTCACAGATTTTCGCCCATACAAAAGTACATTTGAGGGGTATCTTACTTCTGGTCTCGATTTAGATCTTGATCAGCTGGACATTACTTCATTTGTATGGGAAAAGGGACCTCGACTGAGAATTTCTCTGAAGCTTTTTCCTGTATATGTTGATAAGGCCAATAATTCCCATATTTTCAATACAAGCGAAGTTAAAAGAGTCATGACCAAGTTCACATCATGGAAAATTCCTGACAGTGATGTATTTGGACCCTATGAACTTCTCGGCTTCATTCTACTGGAGCCTTACAAGGATGTGGTTGCCCTCTCTTCAAATTCCGGTCTAAGCAAGGGCGCTCTGGCTGGCATAATATTAGGGACTATTGCTGGTGCCGTCGCATTATCTGCAATTGTTTCTCTTCTTATATTCAGAAAACATTTGAAGGACCGCCATGCAATTTCAAGAAGACGTCACACATCCAAGAGCTCCATAAAAATTGAAGGTGTCAAGGCTTTTACTTATGGAGAAATGTCCACTGCTACAAACAAGTTTAACAGCTCTGCTCAAATTGGACAAGGAGGTTATGGAAGGGTTTATAAAGGCACCCTTGCTGATGGAACAGTTGTGGCCATAAAACGTGCACAAGAGGGATCATTACAGGGCGAGAAGGAGTTTCTAACAGAAATAGAATTATTGTCACCCTTACATCATAGGAATCTCGTGTCCTTAGTCGGGTATTGTGATGAAGAAGGGGAACAGATGTTGGTTTATGAGTTCATGTCCAACGGAACTCTAAGGGATCACCTTTCTGCTAGGTCTAAAGAACCGCTTGGTTTTGAGATGAGATTAAGAATTGCCCTGGGATCAGCCAAGGGCATCCTATACCTCCATACAGAAGCCAACCCTCCGATATTTCATCGAGATATCAAGGCCAGCAATATATTATTGGACTCTAAGTTTATTGCAAAGGTTGCTGATTTTGGACTTTCCCGACTTGCCCCAGTTCCAGATTTCGAAGGGACTGTGCCTGCTCATGTATCCACAGTAGTGAAGGGGACACCGGGTTACCTTGATCCAGAGTACTTCTTAACTCATAAGCTGACGGATAAGAGCGATGTTTATAGCCTCGGTGTTGTGTTTTTGGAGCTCTTGACAGGGATGCAGCCAATCTCACATGGCAAAAACATTGTTCGAGAGGTAAACATTGCATTCCAATCCGGTATGATCTTCTCAGTAATTGATGGAAGAATGGGGTCTTATCCTTCCGAGTGTGTGGAAAAGTTCTTGAGTTTGGCTCTCAAGTGTTGCGAAGATGATACAGACGCGAGACCCTCAATGGCAGAGGTTGTTCGGGAACTCGAAAACATATGGTTTATGATGCCGGAGTCTGACAGCAAAACAACGGAATCAGTGACCGGTGGTAGTGGTGGAAAGGTAGAGACTCCACCATCCTCATCCAATGCAGTGAGGCATCCTTACGTCTCTTCAGACGTCTCCGGCAGCGACCTTGTCAGCGGAGTCGTACCTACAATTACGCCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

954

Amino Acids

104.51

Weight (kDa)

6.21

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 72 1.8e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 154 - 214 2.1e-06 Leucine rich repeat
LRR_8 PF13855 298 - 347 4.6e-06 Leucine rich repeat
Pkinase PF00069 624 - 890 3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 626 - 892 1.8e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1914
AatII GACGTC 2 cut(s) 1789, 2811
AccB1I GGYRCC 2 cut(s) 1706, 1919
AccB7I CCANNNNNTGG 2 cut(s) 305, 827
AccI GTMKAC 2 cut(s) 364, 837
AccII CGCG 2 cut(s) 1046, 2642
AclWI GGATC 7 cut(s) 140, 858, 871, 1975, 2124, 2188, 2384
AcoI YGGCCR 2 cut(s) 307, 1944
AcsI RAATTY 7 cut(s) 526, 579, 936, 1159, 1458, 1563, 1651
AcuI CTGAAG 5 cut(s) 462, 953, 1302, 1487, 2787
AcyI GRCGYC 2 cut(s) 1786, 2808
AfaI GTAC 5 cut(s) 190, 1105, 1366, 2399, 2841
AfiI CCNNNNNNNGG 4 cut(s) 190, 305, 827, 2049
AflIII ACRYGT 1 cut(s) 747
AgeI ACCGGT 1 cut(s) 2732
AjnI CCWGG 2 cut(s) 940, 2175
AleI CACNNNNGTG 1 cut(s) 2363
AloI GAACNNNNNNTCC 2 cut(s) 390, 422
Alw21I GWGCWC 6 cut(s) 79, 450, 1303, 1805, 1960, 2466
Alw26I GTCTC 6 cut(s) 174, 1397, 2638, 2750, 2803, 2815
Alw44I GTGCAC 1 cut(s) 1956
AlwI GGATC 7 cut(s) 140, 858, 871, 1975, 2124, 2188, 2384
AoxI GGCC 6 cut(s) 123, 307, 794, 1494, 1944, 2247
ApaLI GTGCAC 1 cut(s) 1956
ApeKI GCWGC 4 cut(s) 118, 1016, 2479, 2817
ApoI RAATTY 7 cut(s) 526, 579, 936, 1159, 1458, 1563, 1651
AseI ATTAAT 1 cut(s) 686
AsiGI ACCGGT 1 cut(s) 2732
Asp700I GAANNNNTTC 1 cut(s) 1275
AspLEI GCGC 2 cut(s) 118, 1673
AspS9I GGNCC 7 cut(s) 123, 503, 794, 1185, 1445, 1586, 1762
AsuC2I CCSGG 2 cut(s) 1335, 2379
AsuHPI GGTGA 5 cut(s) 85, 288, 1208, 2007, 2111
AsuII TTCGAA 2 cut(s) 1269, 2331
AvaII GGWCC 5 cut(s) 503, 1185, 1445, 1586, 1762
BaeGI GKGCMC 1 cut(s) 1960
BaeI ACNNNNGTAYC 2 cut(s) 1087, 1120
BalI TGGCCA 1 cut(s) 1946
BamHI GGATCC 1 cut(s) 863
BanI GGYRCC 2 cut(s) 1706, 1919
BanII GRGCYC 3 cut(s) 450, 1805, 2466
BauI CACGAG 1 cut(s) 2036
BbsI GAAGAC 2 cut(s) 51, 1789
Bbv12I GWGCWC 6 cut(s) 79, 450, 1303, 1805, 1960, 2466
BbvI GCAGC 4 cut(s) 105, 1028, 2491, 2829
BccI CCATC 7 cut(s) 16, 134, 230, 710, 1925, 2552, 2773
BceAI ACGGC 4 cut(s) 129, 207, 1114, 1694
BciT130I CCWGG 2 cut(s) 942, 2177
BciVI GTATCC 2 cut(s) 15, 2365
BclI TGATCA 2 cut(s) 889, 1408
BcnI CCSGG 2 cut(s) 1335, 2379
BcoDI GTCTC 6 cut(s) 174, 1397, 2638, 2750, 2803, 2815
BfaI CTAG 1 cut(s) 2130
BfmI CTRYAG 2 cut(s) 102, 981
BfoI RGCGCY 2 cut(s) 119, 1674
BfuI GTATCC 2 cut(s) 15, 2365
BglII AGATCT 2 cut(s) 82, 1402
BisI GCNGC 5 cut(s) 119, 310, 1017, 2480, 2818
BlsI GCNGC 5 cut(s) 120, 311, 1018, 2481, 2819
BmcAI AGTACT 1 cut(s) 2399
Bme1390I CCNGG 4 cut(s) 942, 1335, 2177, 2379
Bme18I GGWCC 5 cut(s) 503, 1185, 1445, 1586, 1762
BmgT120I GGNCC 7 cut(s) 123, 503, 794, 1185, 1445, 1586, 1762
BmiI GGNNCC 9 cut(s) 433, 865, 1006, 1186, 1446, 1588, 1620, 1708, 1921
BmrFI CCNGG 4 cut(s) 942, 1335, 2177, 2379
BmrI ACTGGG 1 cut(s) 2312
BmsI GCATC 4 cut(s) 2202, 2466, 2685, 2797
BmuI ACTGGG 1 cut(s) 2312
BpiI GAAGAC 2 cut(s) 51, 1789
BplI GAGNNNNNCTC 4 cut(s) 1447, 1479, 2596, 2628
BpmI CTGGAG 2 cut(s) 93, 1637
Bpu10I CCTNAGC 2 cut(s) 588, 842
Bpu14I TTCGAA 2 cut(s) 1269, 2331
BpuEI CTTGAG 2 cut(s) 2600, 2623
BpuMI CCSGG 2 cut(s) 1335, 2379
BsaBI GATNNNNATC 3 cut(s) 240, 1401, 1407
BsaHI GRCGYC 2 cut(s) 1786, 2808
BsaI GGTCTC 3 cut(s) 174, 1397, 2638
BsaJI CCNNGG 6 cut(s) 393, 826, 2175, 2176, 2187, 2443
BsaWI WCCGGW 4 cut(s) 929, 1655, 2534, 2732
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 305, 827, 2049
Bse118I RCCGGY 1 cut(s) 2732
Bse1I ACTGG 5 cut(s) 173, 203, 1164, 1620, 2318
Bse3DI GCAATG 1 cut(s) 2520
Bse8I GATNNNNATC 3 cut(s) 240, 1401, 1407
BseBI CCWGG 2 cut(s) 942, 2177
BseDI CCNNGG 6 cut(s) 393, 826, 2175, 2176, 2187, 2443
BseGI GGATG 7 cut(s) 1636, 1793, 2193, 2481, 2765, 2771, 2788
BseJI GATNNNNATC 3 cut(s) 240, 1401, 1407
BseLI CCNNNNNNNGG 4 cut(s) 190, 305, 827, 2049
BseMI GCAATG 1 cut(s) 2520
BseMII CTCAG 4 cut(s) 152, 579, 1445, 2562
BseNI ACTGG 5 cut(s) 173, 203, 1164, 1620, 2318
BseRI GAGGAG 3 cut(s) 192, 398, 1293
BseSI GKGCMC 1 cut(s) 1960
BseXI GCAGC 4 cut(s) 105, 1028, 2491, 2829
Bsh1236I CGCG 2 cut(s) 1046, 2642
BshFI GGCC 6 cut(s) 125, 309, 796, 1496, 1946, 2249
BshNI GGYRCC 2 cut(s) 1706, 1919
BshTI ACCGGT 1 cut(s) 2732
BsiHKAI GWGCWC 6 cut(s) 79, 450, 1303, 1805, 1960, 2466
BsiSI CCGG 8 cut(s) 930, 1335, 1656, 2378, 2535, 2699, 2733, 2814
BslFI GGGAC 7 cut(s) 733, 1198, 1317, 1458, 1708, 2350, 2386
BslI CCNNNNNNNGG 4 cut(s) 190, 305, 827, 2049
BsmAI GTCTC 6 cut(s) 174, 1397, 2638, 2750, 2803, 2815
BsmBI CGTCTC 2 cut(s) 2803, 2815
BsmFI GGGAC 7 cut(s) 733, 1198, 1317, 1458, 1708, 2350, 2386
BsmI GAATGC 1 cut(s) 2525
BsnI GGCC 6 cut(s) 125, 309, 796, 1496, 1946, 2249
Bso31I GGTCTC 3 cut(s) 174, 1397, 2638
Bsp119I TTCGAA 2 cut(s) 1269, 2331
Bsp1286I GDGCHC 6 cut(s) 79, 450, 1303, 1805, 1960, 2466
BspANI GGCC 6 cut(s) 125, 309, 796, 1496, 1946, 2249
BspCNI CTCAG 4 cut(s) 153, 580, 1446, 2561
BspFNI CGCG 2 cut(s) 1046, 2642
BspHI TCATGA 1 cut(s) 1539
BspLI GGNNCC 9 cut(s) 433, 865, 1006, 1186, 1446, 1588, 1620, 1708, 1921
BspMAI CTGCAG 1 cut(s) 106
BspPI GGATC 7 cut(s) 140, 858, 871, 1975, 2124, 2188, 2384
BspQI GCTCTTC 1 cut(s) 438
BspT104I TTCGAA 2 cut(s) 1269, 2331
BspT107I GGYRCC 2 cut(s) 1706, 1919
BspTNI GGTCTC 3 cut(s) 174, 1397, 2638
BsrDI GCAATG 1 cut(s) 2520
BsrFI RCCGGY 1 cut(s) 2732
BsrI ACTGG 5 cut(s) 173, 203, 1164, 1620, 2318
BssAI RCCGGY 1 cut(s) 2732
BssECI CCNNGG 6 cut(s) 393, 826, 2175, 2176, 2187, 2443
BssNAI GTATAC 1 cut(s) 365
BssNI GRCGYC 2 cut(s) 1786, 2808
BssSI CACGAG 1 cut(s) 2036
BssT1I CCWWGG 3 cut(s) 393, 826, 2187
Bst1107I GTATAC 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 2036
Bst2UI CCWGG 2 cut(s) 942, 2177
Bst4CI ACNGT 8 cut(s) 16, 1129, 1211, 1241, 1574, 1939, 2341, 2362
Bst6I CTCTTC 4 cut(s) 438, 1651, 1740, 2806
BstACI GRCGYC 2 cut(s) 1786, 2808
BstAPI GCANNNNNTGC 2 cut(s) 1721, 2288
BstBI TTCGAA 2 cut(s) 1269, 2331
BstC8I GCNNGC 6 cut(s) 123, 575, 1255, 1681, 2251, 2346
BstEII GGTNACC 1 cut(s) 2381
BstF5I GGATG 7 cut(s) 1636, 1793, 2193, 2481, 2765, 2771, 2788
BstFNI CGCG 2 cut(s) 1046, 2642
BstH2I RGCGCY 2 cut(s) 119, 1674
BstHHI GCGC 2 cut(s) 118, 1673
BstMAI GTCTC 6 cut(s) 174, 1397, 2638, 2750, 2803, 2815
BstMWI GCNNNNNNNGC 4 cut(s) 122, 445, 1721, 2288
BstNI CCWGG 2 cut(s) 942, 2177
BstNSI RCATGY 3 cut(s) 46, 751, 1257
BstPI GGTNACC 1 cut(s) 2381
BstSCI CCNGG 4 cut(s) 940, 1333, 2175, 2377
BstSFI CTRYAG 2 cut(s) 102, 981
BstSLI GKGCMC 1 cut(s) 1960
BstUI CGCG 2 cut(s) 1046, 2642
BstV1I GCAGC 4 cut(s) 105, 1028, 2491, 2829
BstV2I GAAGAC 2 cut(s) 51, 1789
BstX2I RGATCY 3 cut(s) 82, 863, 1402
BstYI RGATCY 3 cut(s) 82, 863, 1402
BstZ17I GTATAC 1 cut(s) 365
BsuI GTATCC 2 cut(s) 15, 2365
BsuRI GGCC 6 cut(s) 125, 309, 796, 1496, 1946, 2249
BtgZI GCGATG 1 cut(s) 2445
BtsCI GGATG 7 cut(s) 1636, 1793, 2193, 2481, 2765, 2771, 2788
BtsI GCAGTG 3 cut(s) 1230, 1851, 2787
BtsIMutI CAGTG 6 cut(s) 1125, 1230, 1579, 1851, 2733, 2787
Cac8I GCNNGC 6 cut(s) 123, 575, 1255, 1681, 2251, 2346
CciI TCATGA 1 cut(s) 1539
CfoI GCGC 2 cut(s) 118, 1673
Cfr10I RCCGGY 1 cut(s) 2732
Cfr13I GGNCC 7 cut(s) 123, 503, 794, 1185, 1445, 1586, 1762
CseI GACGC 1 cut(s) 2648
Csp6I GTAC 5 cut(s) 189, 1104, 1365, 2398, 2840
CspAI ACCGGT 1 cut(s) 2732
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CviQI GTAC 5 cut(s) 189, 1104, 1365, 2398, 2840
EaeI YGGCCR 2 cut(s) 307, 1944
Eam1104I CTCTTC 4 cut(s) 438, 1651, 1740, 2806
EarI CTCTTC 4 cut(s) 438, 1651, 1740, 2806
EciI GGCGGA 1 cut(s) 645
Ecl136II GAGCTC 3 cut(s) 448, 1803, 2464
Eco130I CCWWGG 3 cut(s) 393, 826, 2187
Eco24I GRGCYC 3 cut(s) 450, 1805, 2466
Eco31I GGTCTC 3 cut(s) 174, 1397, 2638
Eco32I GATATC 1 cut(s) 2242
Eco47I GGWCC 5 cut(s) 503, 1185, 1445, 1586, 1762
Eco53kI GAGCTC 3 cut(s) 448, 1803, 2464
Eco57I CTGAAG 5 cut(s) 462, 953, 1302, 1487, 2787
Eco91I GGTNACC 1 cut(s) 2381
EcoICRI GAGCTC 3 cut(s) 448, 1803, 2464
EcoO109I RGGNCCY 2 cut(s) 794, 1445
EcoO65I GGTNACC 1 cut(s) 2381
EcoRI GAATTC 1 cut(s) 936
EcoRII CCWGG 2 cut(s) 940, 2175
EcoRV GATATC 1 cut(s) 2242
EcoT14I CCWWGG 3 cut(s) 393, 826, 2187
EcoT22I ATGCAT 1 cut(s) 31
EcoT38I GRGCYC 3 cut(s) 450, 1805, 2466
ErhI CCWWGG 3 cut(s) 393, 826, 2187
Esp3I CGTCTC 2 cut(s) 2803, 2815
FalI AAGNNNNNCTT 4 cut(s) 1908, 1940, 2130, 2162
FaqI GGGAC 7 cut(s) 733, 1198, 1317, 1458, 1708, 2350, 2386
FauI CCCGC 2 cut(s) 114, 566
FauNDI CATATG 2 cut(s) 25, 2684
FbaI TGATCA 2 cut(s) 889, 1408
FblI GTMKAC 2 cut(s) 364, 837
Fnu4HI GCNGC 5 cut(s) 119, 310, 1017, 2480, 2818
FokI GGATG 7 cut(s) 1643, 1780, 2180, 2488, 2752, 2758, 2775
FriOI GRGCYC 3 cut(s) 450, 1805, 2466
Fsp4HI GCNGC 5 cut(s) 119, 310, 1017, 2480, 2818
FspBI CTAG 1 cut(s) 2130
GlaI GCGC 2 cut(s) 117, 1672
GluI GCNGC 5 cut(s) 119, 310, 1017, 2480, 2818
GsuI CTGGAG 2 cut(s) 93, 1637
HaeII RGCGCY 2 cut(s) 119, 1674
HaeIII GGCC 6 cut(s) 125, 309, 796, 1496, 1946, 2249
HapII CCGG 8 cut(s) 930, 1335, 1656, 2378, 2535, 2699, 2733, 2814
HgaI GACGC 1 cut(s) 2648
HhaI GCGC 2 cut(s) 118, 1673
Hin1I GRCGYC 2 cut(s) 1786, 2808
Hin6I GCGC 2 cut(s) 116, 1671
HinP1I GCGC 2 cut(s) 116, 1671
HincII GTYRAC 1 cut(s) 700
HindII GTYRAC 1 cut(s) 700
HindIII AAGCTT 2 cut(s) 1284, 1469
HpaII CCGG 8 cut(s) 930, 1335, 1656, 2378, 2535, 2699, 2733, 2814
HphI GGTGA 5 cut(s) 85, 288, 1208, 2007, 2111
Hpy99I CGWCG 2 cut(s) 904, 1715
HpyAV CCTTC 9 cut(s) 364, 1753, 1811, 1899, 1978, 2060, 2327, 2362, 2589
HpyCH4III ACNGT 8 cut(s) 16, 1129, 1211, 1241, 1574, 1939, 2341, 2362
HpyCH4IV ACGT 4 cut(s) 1786, 1954, 2796, 2808
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 445, 1721, 2288
HpySE526I ACGT 4 cut(s) 1786, 1954, 2796, 2808
Hsp92I GRCGYC 2 cut(s) 1786, 2808
HspAI GCGC 2 cut(s) 116, 1671
Ksp22I TGATCA 2 cut(s) 889, 1408
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 7 cut(s) 74, 411, 1010, 1306, 1618, 1808, 2461
Lsp1109I GCAGC 4 cut(s) 105, 1028, 2491, 2829
LweI GCATC 4 cut(s) 2202, 2466, 2685, 2797
MaeI CTAG 1 cut(s) 2130
MaeII ACGT 4 cut(s) 1786, 1954, 2796, 2808
MaeIII GTNAC 8 cut(s) 109, 294, 1123, 1196, 1787, 2013, 2381, 2728
MfeI CAATTG 1 cut(s) 1725
MflI RGATCY 3 cut(s) 82, 863, 1402
MhlI GDGCHC 6 cut(s) 79, 450, 1303, 1805, 1960, 2466
MlsI TGGCCA 1 cut(s) 1946
MluNI TGGCCA 1 cut(s) 1946
MlyI GAGTC 7 cut(s) 616, 1255, 1545, 2261, 2711, 2752, 2844
MmeI TCCRAC 1 cut(s) 2124
Mox20I TGGCCA 1 cut(s) 1946
Mph1103I ATGCAT 1 cut(s) 31
MroXI GAANNNNTTC 1 cut(s) 1275
MscI TGGCCA 1 cut(s) 1946
MseI TTAA 6 cut(s) 686, 1224, 1530, 1869, 2165, 2405
MslI CAYNNNNRTG 3 cut(s) 28, 1432, 2363
Msp20I TGGCCA 1 cut(s) 1946
MspA1I CMGCKG 2 cut(s) 1414, 2832
MspI CCGG 8 cut(s) 930, 1335, 1656, 2378, 2535, 2699, 2733, 2814
MspR9I CCNGG 4 cut(s) 942, 1335, 2177, 2379
MunI CAATTG 1 cut(s) 1725
Mva1269I GAATGC 1 cut(s) 2525
MvaI CCWGG 2 cut(s) 942, 2177
MvnI CGCG 2 cut(s) 1046, 2642
MwoI GCNNNNNNNGC 4 cut(s) 122, 445, 1721, 2288
NciI CCSGG 2 cut(s) 1335, 2379
NdeI CATATG 2 cut(s) 25, 2684
NlaIV GGNNCC 9 cut(s) 433, 865, 1006, 1186, 1446, 1588, 1620, 1708, 1921
NmeAIII GCCGAG 1 cut(s) 1581
NmuCI GTSAC 7 cut(s) 109, 294, 1123, 1196, 1787, 2013, 2728
NsiI ATGCAT 1 cut(s) 31
NspI RCATGY 3 cut(s) 46, 751, 1257
NspV TTCGAA 2 cut(s) 1269, 2331
OliI CACNNNNGTG 1 cut(s) 2363
PaeI GCATGC 1 cut(s) 1257
PagI TCATGA 1 cut(s) 1539
PasI CCCWGGG 1 cut(s) 2176
PciI ACATGT 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 438
PctI GAATGC 1 cut(s) 2525
PdmI GAANNNNTTC 1 cut(s) 1275
PfeI GAWTC 8 cut(s) 157, 172, 277, 734, 817, 1338, 2032, 2723
PflFI GACNNNGTC 1 cut(s) 2825
PflMI CCANNNNNTGG 2 cut(s) 305, 827
PfoI TCCNGGA 1 cut(s) 1333
PinAI ACCGGT 1 cut(s) 2732
PkrI GCNGC 5 cut(s) 120, 311, 1018, 2481, 2819
PleI GAGTC 7 cut(s) 615, 1254, 1544, 2261, 2710, 2752, 2843
PpsI GAGTC 7 cut(s) 615, 1254, 1544, 2261, 2710, 2752, 2843
PpuMI RGGWCCY 1 cut(s) 1445
PscI ACATGT 1 cut(s) 747
PshBI ATTAAT 1 cut(s) 686
PsiI TTATAA 1 cut(s) 1914
Psp124BI GAGCTC 3 cut(s) 450, 1805, 2466
Psp5II RGGWCCY 1 cut(s) 1445
Psp6I CCWGG 2 cut(s) 940, 2175
PspEI GGTNACC 1 cut(s) 2381
PspGI CCWGG 2 cut(s) 940, 2175
PspN4I GGNNCC 9 cut(s) 433, 865, 1006, 1186, 1446, 1588, 1620, 1708, 1921
PspPI GGNCC 7 cut(s) 123, 503, 794, 1185, 1445, 1586, 1762
PspPPI RGGWCCY 1 cut(s) 1445
PstI CTGCAG 1 cut(s) 106
PsuI RGATCY 3 cut(s) 82, 863, 1402
PsyI GACNNNGTC 1 cut(s) 2825
PvuII CAGCTG 1 cut(s) 1414
RsaI GTAC 5 cut(s) 190, 1105, 1366, 2399, 2841
RsaNI GTAC 5 cut(s) 189, 1104, 1365, 2398, 2840
RseI CAYNNNNRTG 3 cut(s) 28, 1432, 2363
SacI GAGCTC 3 cut(s) 450, 1805, 2466
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 6 cut(s) 686, 1224, 1530, 1869, 2165, 2405
SatI GCNGC 5 cut(s) 119, 310, 1017, 2480, 2818
Sau96I GGNCC 7 cut(s) 123, 503, 794, 1185, 1445, 1586, 1762
ScaI AGTACT 1 cut(s) 2399
SchI GAGTC 7 cut(s) 616, 1255, 1545, 2261, 2711, 2752, 2844
ScrFI CCNGG 4 cut(s) 942, 1335, 2177, 2379
SduI GDGCHC 6 cut(s) 79, 450, 1303, 1805, 1960, 2466
SfaNI GCATC 4 cut(s) 2202, 2466, 2685, 2797
SfcI CTRYAG 2 cut(s) 102, 981
SfuI TTCGAA 2 cut(s) 1269, 2331
SinI GGWCC 5 cut(s) 503, 1185, 1445, 1586, 1762
SmiMI CAYNNNNRTG 3 cut(s) 28, 1432, 2363
SmlI CTYRAG 2 cut(s) 2602, 2615
SmoI CTYRAG 2 cut(s) 2602, 2615
SphI GCATGC 1 cut(s) 1257
SspI AATATT 2 cut(s) 330, 1689
SspMI CTAG 1 cut(s) 2130
SstI GAGCTC 3 cut(s) 450, 1805, 2466
StyD4I CCNGG 4 cut(s) 940, 1333, 2175, 2377
StyI CCWWGG 3 cut(s) 393, 826, 2187
TaaI ACNGT 8 cut(s) 16, 1129, 1211, 1241, 1574, 1939, 2341, 2362
TaiI ACGT 4 cut(s) 1789, 1957, 2799, 2811
TaqII GACCGA 1 cut(s) 1202
TatI WGTACW 2 cut(s) 1364, 2397
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 8 cut(s) 157, 172, 277, 734, 817, 1338, 2032, 2723
Tru1I TTAA 6 cut(s) 686, 1224, 1530, 1869, 2165, 2405
Tru9I TTAA 6 cut(s) 686, 1224, 1530, 1869, 2165, 2405
TscAI CASTG 6 cut(s) 1132, 1237, 1579, 1858, 2733, 2787
TseFI GTSAC 7 cut(s) 109, 294, 1123, 1196, 1787, 2013, 2728
TseI GCWGC 4 cut(s) 118, 1016, 2479, 2817
Tsp45I GTSAC 7 cut(s) 109, 294, 1123, 1196, 1787, 2013, 2728
TspGWI ACGGA 4 cut(s) 429, 2118, 2435, 2735
TspRI CASTG 6 cut(s) 1132, 1237, 1579, 1858, 2733, 2787
Tth111I GACNNNGTC 1 cut(s) 2825
Van91I CCANNNNNTGG 2 cut(s) 305, 827
VneI GTGCAC 1 cut(s) 1956
VpaK11BI GGWCC 5 cut(s) 503, 1185, 1445, 1586, 1762
VspI ATTAAT 1 cut(s) 686
XapI RAATTY 7 cut(s) 526, 579, 936, 1159, 1458, 1563, 1651
XceI RCATGY 3 cut(s) 46, 751, 1257
XcmI CCANNNNNNNNNTGG 2 cut(s) 15, 1020
XmiI GTMKAC 2 cut(s) 364, 837
XmnI GAANNNNTTC 1 cut(s) 1275
XspI CTAG 1 cut(s) 2130
ZraI GACGTC 2 cut(s) 1787, 2809
ZrmI AGTACT 1 cut(s) 2399
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.