MD03G1048800.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005593
Physical Location & Seq
Reverse (-)
3921490 .. 3928273
6784 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1048800.v1.1.491

Sequence Viewer

Length: 2862 bp
ATGCATCAATCAAGAGTTTGGACATATGCATTTGTTTTCGCCACGTTCCTGTGCTGGTCTTCACTACGCGCTGGAGCACAAAATCCTAATATCACCAACCCTGCAGAAGTGACCGCGCTGCGAGCCATCAAAAGCAGTTTGGTTGATCCCAACAAGAATCTGAGCAACTGGAATAGAGGAGACCCATGCACAGCAAATTGGACAGGAGTGTTTTGCTACAACACAACATTAGATGATGGCTATCTTCATGTTCAACAACTGCGACTACTAAATATGAACCTGTCAGGAAGTTTATCGCCAGAGCTCGGCCGCTTATCTTATATGAAGATACTGGATTTTATGTGGAACAAAATAAGTGGAAGTATACCAAAGGAGATAGGCAACATTACATCCTTGGAACTTTTGCTCCTCAATGGAAACCAGTTATCAGGTTCCTTGCCCGAAAAGCTTGGTTATCTTCCAAACTTGGACAGATTACAAATTGACGAGAACCAAATATCGGGATCAATACCTATATCGTTTGCAAATTTAAACAAGACAAGGCATTTTCACATGAACAACAATTCAATAAGCGGGCAAATTCCACCGGAGCTTTCCAGATTACCAAGGCTTATTCACTTTCTTCTTGACAACAACAACTTATCTGGGTATCTTCCACCGCAGTTCTCGGAGTTGCCAAGCTTATTAATACTTCAACTTGACAACAACAACTTTGAAGGGACTACAATTCCAGATTCGTATAGCGACATGTCTAAACTGTTGAAGTTGAGTTTGAGGAACTGCAACTTACAAGGGCCTATTCCTGATTTGAGTAGGATTCCAAACCTTGCTTATATAGACCTAAGCTCAAATCGGCTAAACGGATCCTTGCCTCAGGGTACACTTTCTGATAACATCACGACGATCAATTTATCTAACAACACTCTGACCGGAACAATTCCTGGCAACTTTTCGGATCTTCCTAATCTTCAGAGATTGTCTATAGCAAACAATTCATTGAATGGCTCCATTCCAGCAACGCTTTGGGAAAACAGGAAACTCAACGGGACAGAAAGACTAATCATGGAGATGCAGAACAACAACCTCACAAACATTACCGGCAGTACTGTACTTCCGCAAAATGTCACTGTCTGGCTTCGGGGAAATCCGGTTTGCTCAAATGGCAACCTTGTTAACTTCTGCGGAACAGAAAGTGATGATGAAAATGACAGTCATATTTCGACCAACTCCACTGCCAACTGTCCGAATCAAGCATGTCCACCCGCTTTCGAATATATTCCTGTAGCTTGTTTTTGTGCTGCTCCTCTAATTGTTGACATTCGGTTGAAAAGTCCCGCATTCACAGATTTTCGCCCTTACGAAAGTACATTTGAGCAGTATCTTACTTCTGGTATCAAATTAGATCTCGATCAGCTGGACATTACTTCATTTGTATGGGAAAACGGACCTCGACTGAGAATTTCTCTGAAGCTTTTTCCTGTATATGTTGAAAATGCCAATAATTCCCATACTTTCAATAGAACTGAAGTTAAAAGAATCATGAATATGTTCGCATCATGGGCAATTCCTGACAGTGGCCTATTTGGACCCTATGAACTTCTCGACTTCATTCTACTGGATCCTTACAAGGATGTGGTTGCCTCCTCTTCAAAGTCCGGTCTAGGCAAAGGTGCTCTGGCTGGCATCATACTAGGGACTATTGCTGGTGCCGTTACAGTATCTGCAATTGTTTCTCTTCTTATATTGAGAAAGCATTTGAAGGATCGCCATGCAATTTCAAGAAGACGTCGTACAACCAAGAGCTCCATAAAAATTGAAGGTGTCAAGGATTTTACTTTTAGAGAAATGGCCGCGGCTACAAACAACTTTAACAGCTCTGCTCAAGTTGGACAAGGAGGTTATGGAAATGTTTATAAAGGCATCCTGGCTGACGGAACCGTTGTGGCCATAAAACGTGCACAAGAGGGATCATTACAGGGCGAGAAGGAGTTTCTAACAGAAATAGAATTATTGTCACCCTTACATCATAGGAATCTCGTGTCCTTAGTCGGGTATTGTGATGAAGAAGGGGAACAGATGTTGGTTTATGAGTTTATGTCCAATGGAACTCTAAGGGATCACCTTTCTGCTAGGTCTAAAGAACCACTGGGTTTTGAGATGAGATTAAGAATTGCTCAGGGATCAGCCAAGGGCATCCTATACCTCCATACAGAAGCCAACCCTCCGATATTTCATCGAGATATCAAGGCCACCAATATATTATTGGACTCTAAGTTCATGGCAAAGGTTGCTGATTTTGGCCTTTCCCGTCTTGCCCCGGTTCCAGATTTTGAAGGGACTGTGCCTGCTCATGTATCCACAGTAGTGAAGGGGACACCGGGTTACCTTGATCCGGAGTACTTCTTAACTCATAAGCTAACGGATAAGAGCGATGTTTATAGCCTTGGTGTTGTGTTTTTGGAGCTCTTAACAGGCATGCAGCCAATCTCACATGGCAAAAACATTGTTCGAGAGGTAAACATTGCATTCCAATCCGGTATGATCTTCTCAGTTATTGATGGAAGAATGGGGTCTTATCCTTCCGAGTGTGTGGAAAAGTTCTTGAGTTTGGCTCTCAAGTGTTGCAAAGAGGAGACAGACGCGAGACCCTCAATGGCGGAGGTTGTTCGGGAACTCGAGAACATATGGTTTATGATGCCGGAGTCTGACAGCAGAACATCGGAATCAGTGACGAGCACTAGTGGTGGAAAGGTGGTGACTCTTCCACCCTCATCCAATGCAGCGAGGCATCCTTACGTCTCTATAGACGTCTCTGGCAGTGACCTTGTTAGCGGAGTCGTACCGACAATTAGACCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

954

Amino Acids

105.1

Weight (kDa)

6.18

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 72 6.8e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 274 - 333 2e-08 Leucine rich repeat
LRR_8 PF13855 298 - 347 1.6e-06 Leucine rich repeat
Pkinase PF00069 624 - 890 3.5e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 626 - 892 2.9e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1914
AatII GACGTC 2 cut(s) 1789, 2811
AccB1I GGYRCC 1 cut(s) 1706
AccI GTMKAC 1 cut(s) 364
AccII CGCG 4 cut(s) 69, 116, 1853, 2642
AccIII TCCGGA 1 cut(s) 2392
AcoI YGGCCR 3 cut(s) 307, 1848, 1944
AcsI RAATTY 3 cut(s) 526, 579, 1458
AcuI CTGAAG 3 cut(s) 953, 1487, 1545
AcyI GRCGYC 2 cut(s) 1786, 2808
AfaI GTAC 7 cut(s) 880, 1105, 1110, 1366, 1792, 2399, 2841
AfiI CCNNNNNNNGG 5 cut(s) 305, 499, 1574, 2049, 2392
AflIII ACRYGT 1 cut(s) 747
AhlI ACTAGT 1 cut(s) 2738
AjnI CCWGG 2 cut(s) 940, 1923
AleI CACNNNNGTG 1 cut(s) 2363
AloI GAACNNNNNNTCC 4 cut(s) 390, 422, 2258, 2290
Alw21I GWGCWC 7 cut(s) 79, 306, 1675, 1805, 1960, 2466, 2738
Alw26I GTCTC 5 cut(s) 174, 2627, 2638, 2803, 2815
Alw44I GTGCAC 1 cut(s) 1956
AlwNI CAGNNNCTG 1 cut(s) 1721
Ama87I CYCGRG 1 cut(s) 2675
Aor13HI TCCGGA 1 cut(s) 2392
AoxI GGCC 7 cut(s) 307, 794, 1576, 1848, 1944, 2247, 2299
ApaLI GTGCAC 1 cut(s) 1956
ApeKI GCWGC 4 cut(s) 118, 1298, 2479, 2780
ApoI RAATTY 3 cut(s) 526, 579, 1458
AseI ATTAAT 1 cut(s) 686
Asp700I GAANNNNTTC 2 cut(s) 1275, 1547
AspLEI GCGC 2 cut(s) 71, 118
AspS9I GGNCC 3 cut(s) 794, 1445, 1586
AsuC2I CCSGG 2 cut(s) 2318, 2379
AsuHPI GGTGA 4 cut(s) 85, 2007, 2111, 2767
AsuII TTCGAA 1 cut(s) 1269
AvaI CYCGRG 1 cut(s) 2675
AvaII GGWCC 2 cut(s) 1445, 1586
AxyI CCTNAGG 1 cut(s) 873
BaeGI GKGCMC 1 cut(s) 1960
BalI TGGCCA 1 cut(s) 1946
BamHI GGATCC 2 cut(s) 863, 1618
BanI GGYRCC 1 cut(s) 1706
BanII GRGCYC 3 cut(s) 306, 1805, 2466
BarI GAAGNNNNNNTAC 2 cut(s) 1774, 1806
BauI CACGAG 1 cut(s) 2036
BbsI GAAGAC 2 cut(s) 51, 1789
Bbv12I GWGCWC 7 cut(s) 79, 306, 1675, 1805, 1960, 2466, 2738
BbvI GCAGC 4 cut(s) 105, 1285, 2491, 2792
BccI CCATC 3 cut(s) 134, 230, 2552
BceAI ACGGC 1 cut(s) 1694
BciT130I CCWGG 2 cut(s) 942, 1925
BciVI GTATCC 1 cut(s) 2365
BcnI CCSGG 2 cut(s) 2318, 2379
BcoDI GTCTC 5 cut(s) 174, 2627, 2638, 2803, 2815
BcuI ACTAGT 1 cut(s) 2738
BfaI CTAG 4 cut(s) 1661, 1691, 2130, 2739
BfmI CTRYAG 4 cut(s) 102, 981, 1281, 2802
BfuI GTATCC 1 cut(s) 2365
BglII AGATCT 1 cut(s) 1402
BisI GCNGC 7 cut(s) 119, 310, 1299, 1851, 1854, 2480, 2781
BlsI GCNGC 7 cut(s) 120, 311, 1300, 1852, 1855, 2481, 2782
BmcAI AGTACT 2 cut(s) 1105, 2399
Bme1390I CCNGG 4 cut(s) 942, 1925, 2318, 2379
Bme18I GGWCC 2 cut(s) 1445, 1586
BmeT110I CYCGRG 1 cut(s) 2675
BmgT120I GGNCC 3 cut(s) 794, 1445, 1586
BmiI GGNNCC 8 cut(s) 433, 865, 1006, 1588, 1620, 1708, 1936, 2322
BmrFI CCNGG 4 cut(s) 942, 1925, 2318, 2379
BmrI ACTGGG 1 cut(s) 2156
BmsI GCATC 8 cut(s) 13, 1059, 1562, 1692, 1929, 2202, 2685, 2797
BmuI ACTGGG 1 cut(s) 2156
BpiI GAAGAC 2 cut(s) 51, 1789
BplI GAGNNNNNCTC 4 cut(s) 1447, 1479, 2596, 2628
BpmI CTGGAG 1 cut(s) 93
Bpu10I CCTNAGC 2 cut(s) 842, 2175
Bpu14I TTCGAA 1 cut(s) 1269
BpuEI CTTGAG 3 cut(s) 1866, 2600, 2623
BpuMI CCSGG 2 cut(s) 2318, 2379
BsaBI GATNNNNATC 2 cut(s) 240, 1407
BsaHI GRCGYC 2 cut(s) 1786, 2808
BsaI GGTCTC 2 cut(s) 174, 2638
BsaJI CCNNGG 6 cut(s) 393, 605, 1851, 2187, 2316, 2443
BsaWI WCCGGW 6 cut(s) 586, 929, 1147, 1655, 2392, 2534
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 5 cut(s) 305, 499, 1574, 2049, 2392
Bse118I RCCGGY 1 cut(s) 1097
Bse1I ACTGG 5 cut(s) 173, 336, 421, 1620, 2151
Bse21I CCTNAGG 1 cut(s) 873
Bse3DI GCAATG 1 cut(s) 2520
Bse8I GATNNNNATC 2 cut(s) 240, 1407
BseAI TCCGGA 1 cut(s) 2392
BseBI CCWGG 2 cut(s) 942, 1925
BseDI CCNNGG 6 cut(s) 393, 605, 1851, 2187, 2316, 2443
BseGI GGATG 6 cut(s) 389, 1636, 1920, 2193, 2771, 2788
BseJI GATNNNNATC 2 cut(s) 240, 1407
BseLI CCNNNNNNNGG 5 cut(s) 305, 499, 1574, 2049, 2392
BseMI GCAATG 1 cut(s) 2520
BseMII CTCAG 5 cut(s) 152, 887, 1445, 2189, 2562
BseNI ACTGG 5 cut(s) 173, 336, 421, 1620, 2151
BseRI GAGGAG 5 cut(s) 192, 398, 1293, 1633, 2645
BseSI GKGCMC 1 cut(s) 1960
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 4 cut(s) 105, 1285, 2491, 2792
Bsh1236I CGCG 4 cut(s) 69, 116, 1853, 2642
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 7 cut(s) 309, 796, 1578, 1850, 1946, 2249, 2301
BshNI GGYRCC 1 cut(s) 1706
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 7 cut(s) 79, 306, 1675, 1805, 1960, 2466, 2738
BsiHKCI CYCGRG 1 cut(s) 2675
BslFI GGGAC 6 cut(s) 733, 1060, 1317, 1708, 2350, 2386
BslI CCNNNNNNNGG 5 cut(s) 305, 499, 1574, 2049, 2392
BsmAI GTCTC 5 cut(s) 174, 2627, 2638, 2803, 2815
BsmBI CGTCTC 2 cut(s) 2803, 2815
BsmFI GGGAC 6 cut(s) 733, 1060, 1317, 1708, 2350, 2386
BsmI GAATGC 2 cut(s) 1337, 2525
BsnI GGCC 7 cut(s) 309, 796, 1578, 1850, 1946, 2249, 2301
Bso31I GGTCTC 2 cut(s) 174, 2638
BsoBI CYCGRG 1 cut(s) 2675
Bsp119I TTCGAA 1 cut(s) 1269
Bsp1286I GDGCHC 7 cut(s) 79, 306, 1675, 1805, 1960, 2466, 2738
Bsp13I TCCGGA 1 cut(s) 2392
BspANI GGCC 7 cut(s) 309, 796, 1578, 1850, 1946, 2249, 2301
BspCNI CTCAG 5 cut(s) 153, 886, 1446, 2188, 2561
BspEI TCCGGA 1 cut(s) 2392
BspFNI CGCG 4 cut(s) 69, 116, 1853, 2642
BspHI TCATGA 1 cut(s) 1539
BspLI GGNNCC 8 cut(s) 433, 865, 1006, 1588, 1620, 1708, 1936, 2322
BspMAI CTGCAG 1 cut(s) 106
BspT104I TTCGAA 1 cut(s) 1269
BspT107I GGYRCC 1 cut(s) 1706
BspTNI GGTCTC 2 cut(s) 174, 2638
BsrDI GCAATG 1 cut(s) 2520
BsrFI RCCGGY 1 cut(s) 1097
BsrI ACTGG 5 cut(s) 173, 336, 421, 1620, 2151
BssAI RCCGGY 1 cut(s) 1097
BssECI CCNNGG 6 cut(s) 393, 605, 1851, 2187, 2316, 2443
BssNAI GTATAC 1 cut(s) 365
BssNI GRCGYC 2 cut(s) 1786, 2808
BssSI CACGAG 1 cut(s) 2036
BssT1I CCWWGG 4 cut(s) 393, 605, 2187, 2443
Bst1107I GTATAC 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 2036
Bst2UI CCWGG 2 cut(s) 942, 1925
Bst6I CTCTTC 3 cut(s) 1651, 1740, 2766
BstACI GRCGYC 2 cut(s) 1786, 2808
BstAPI GCANNNNNTGC 1 cut(s) 2288
BstBI TTCGAA 1 cut(s) 1269
BstC8I GCNNGC 5 cut(s) 123, 575, 1681, 2346, 2477
BstDEI CTNAG 9 cut(s) 161, 842, 873, 1454, 2044, 2111, 2175, 2271, 2548
BstDSI CCRYGG 1 cut(s) 1851
BstEII GGTNACC 1 cut(s) 2381
BstF5I GGATG 6 cut(s) 389, 1636, 1920, 2193, 2771, 2788
BstFNI CGCG 4 cut(s) 69, 116, 1853, 2642
BstHHI GCGC 2 cut(s) 71, 118
BstMAI GTCTC 5 cut(s) 174, 2627, 2638, 2803, 2815
BstMCI CGRYCG 1 cut(s) 310
BstMWI GCNNNNNNNGC 5 cut(s) 122, 445, 1161, 1559, 2288
BstNI CCWGG 2 cut(s) 942, 1925
BstNSI RCATGY 3 cut(s) 751, 1257, 2479
BstPI GGTNACC 1 cut(s) 2381
BstSCI CCNGG 4 cut(s) 940, 1923, 2316, 2377
BstSFI CTRYAG 4 cut(s) 102, 981, 1281, 2802
BstSLI GKGCMC 1 cut(s) 1960
BstUI CGCG 4 cut(s) 69, 116, 1853, 2642
BstV1I GCAGC 4 cut(s) 105, 1285, 2491, 2792
BstV2I GAAGAC 2 cut(s) 51, 1789
BstX2I RGATCY 4 cut(s) 863, 955, 1402, 1618
BstYI RGATCY 4 cut(s) 863, 955, 1402, 1618
BstZ17I GTATAC 1 cut(s) 365
BstZI CGGCCG 1 cut(s) 307
Bsu36I CCTNAGG 1 cut(s) 873
BsuI GTATCC 1 cut(s) 2365
BsuRI GGCC 7 cut(s) 309, 796, 1578, 1850, 1946, 2249, 2301
BtgI CCRYGG 1 cut(s) 1851
BtgZI GCGATG 1 cut(s) 2445
BtsCI GGATG 6 cut(s) 389, 1636, 1920, 2193, 2771, 2788
BtsI GCAGTG 2 cut(s) 1230, 2824
BtsIMutI CAGTG 6 cut(s) 1125, 1230, 1579, 2144, 2733, 2824
Cac8I GCNNGC 5 cut(s) 123, 575, 1681, 2346, 2477
CaiI CAGNNNCTG 1 cut(s) 1721
CciI TCATGA 1 cut(s) 1539
CfoI GCGC 2 cut(s) 71, 118
Cfr10I RCCGGY 1 cut(s) 1097
Cfr13I GGNCC 3 cut(s) 794, 1445, 1586
Cfr42I CCGCGG 1 cut(s) 1854
CseI GACGC 1 cut(s) 2648
Csp6I GTAC 7 cut(s) 879, 1104, 1109, 1365, 1791, 2398, 2840
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CviQI GTAC 7 cut(s) 879, 1104, 1109, 1365, 1791, 2398, 2840
DdeI CTNAG 9 cut(s) 161, 842, 873, 1454, 2044, 2111, 2175, 2271, 2548
DraI TTTAAA 1 cut(s) 531
EaeI YGGCCR 3 cut(s) 307, 1848, 1944
EagI CGGCCG 1 cut(s) 307
Eam1104I CTCTTC 3 cut(s) 1651, 1740, 2766
EarI CTCTTC 3 cut(s) 1651, 1740, 2766
EciI GGCGGA 1 cut(s) 2672
Ecl136II GAGCTC 3 cut(s) 304, 1803, 2464
EclXI CGGCCG 1 cut(s) 307
Eco130I CCWWGG 4 cut(s) 393, 605, 2187, 2443
Eco24I GRGCYC 3 cut(s) 306, 1805, 2466
Eco31I GGTCTC 2 cut(s) 174, 2638
Eco32I GATATC 1 cut(s) 2242
Eco47I GGWCC 2 cut(s) 1445, 1586
Eco52I CGGCCG 1 cut(s) 307
Eco53kI GAGCTC 3 cut(s) 304, 1803, 2464
Eco57I CTGAAG 3 cut(s) 953, 1487, 1545
Eco81I CCTNAGG 1 cut(s) 873
Eco88I CYCGRG 1 cut(s) 2675
Eco91I GGTNACC 1 cut(s) 2381
EcoICRI GAGCTC 3 cut(s) 304, 1803, 2464
EcoO109I RGGNCCY 1 cut(s) 794
EcoO65I GGTNACC 1 cut(s) 2381
EcoRII CCWGG 2 cut(s) 940, 1923
EcoRV GATATC 1 cut(s) 2242
EcoT14I CCWWGG 4 cut(s) 393, 605, 2187, 2443
EcoT22I ATGCAT 2 cut(s) 6, 31
EcoT38I GRGCYC 3 cut(s) 306, 1805, 2466
ErhI CCWWGG 4 cut(s) 393, 605, 2187, 2443
Esp3I CGTCTC 2 cut(s) 2803, 2815
FaqI GGGAC 6 cut(s) 733, 1060, 1317, 1708, 2350, 2386
FauI CCCGC 3 cut(s) 566, 1270, 1342
FauNDI CATATG 2 cut(s) 25, 2684
FblI GTMKAC 1 cut(s) 364
Fnu4HI GCNGC 7 cut(s) 119, 310, 1299, 1851, 1854, 2480, 2781
FokI GGATG 6 cut(s) 376, 1643, 1907, 2180, 2758, 2775
FriOI GRGCYC 3 cut(s) 306, 1805, 2466
Fsp4HI GCNGC 7 cut(s) 119, 310, 1299, 1851, 1854, 2480, 2781
FspBI CTAG 4 cut(s) 1661, 1691, 2130, 2739
GlaI GCGC 2 cut(s) 70, 117
GluI GCNGC 7 cut(s) 119, 310, 1299, 1851, 1854, 2480, 2781
GsuI CTGGAG 1 cut(s) 93
HaeIII GGCC 7 cut(s) 309, 796, 1578, 1850, 1946, 2249, 2301
HgaI GACGC 1 cut(s) 2648
HhaI GCGC 2 cut(s) 71, 118
Hin1I GRCGYC 2 cut(s) 1786, 2808
Hin6I GCGC 2 cut(s) 69, 116
HinP1I GCGC 2 cut(s) 69, 116
HincII GTYRAC 2 cut(s) 1174, 1315
HindII GTYRAC 2 cut(s) 1174, 1315
HindIII AAGCTT 3 cut(s) 446, 679, 1469
HpaI GTTAAC 1 cut(s) 1174
HphI GGTGA 4 cut(s) 85, 2007, 2111, 2767
Hpy166II GTNNAC 7 cut(s) 365, 881, 1174, 1259, 1315, 1958, 2518
Hpy8I GTNNAC 7 cut(s) 365, 881, 1174, 1259, 1315, 1958, 2518
Hpy99I CGWCG 2 cut(s) 904, 1791
HpyAV CCTTC 8 cut(s) 710, 1753, 1811, 1978, 2060, 2327, 2362, 2589
HpyCH4IV ACGT 5 cut(s) 44, 1786, 1954, 2796, 2808
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 445, 1161, 1559, 2288
HpyF3I CTNAG 9 cut(s) 161, 842, 873, 1454, 2044, 2111, 2175, 2271, 2548
HpySE526I ACGT 5 cut(s) 44, 1786, 1954, 2796, 2808
Hsp92I GRCGYC 2 cut(s) 1786, 2808
HspAI GCGC 2 cut(s) 69, 116
Kpn2I TCCGGA 1 cut(s) 2392
KspAI GTTAAC 1 cut(s) 1174
KspI CCGCGG 1 cut(s) 1854
LmnI GCTCC 7 cut(s) 74, 411, 589, 1010, 1306, 1808, 2461
Lsp1109I GCAGC 4 cut(s) 105, 1285, 2491, 2792
LweI GCATC 8 cut(s) 13, 1059, 1562, 1692, 1929, 2202, 2685, 2797
MaeI CTAG 4 cut(s) 1661, 1691, 2130, 2739
MaeII ACGT 5 cut(s) 44, 1786, 1954, 2796, 2808
MaeIII GTNAC 8 cut(s) 109, 1123, 1711, 2013, 2381, 2728, 2755, 2819
MfeI CAATTG 1 cut(s) 1725
MflI RGATCY 4 cut(s) 863, 955, 1402, 1618
MhlI GDGCHC 7 cut(s) 79, 306, 1675, 1805, 1960, 2466, 2738
MlsI TGGCCA 1 cut(s) 1946
MluNI TGGCCA 1 cut(s) 1946
MlyI GAGTC 4 cut(s) 2261, 2711, 2752, 2844
MmeI TCCRAC 1 cut(s) 1867
Mox20I TGGCCA 1 cut(s) 1946
Mph1103I ATGCAT 2 cut(s) 6, 31
MroI TCCGGA 1 cut(s) 2392
MroXI GAANNNNTTC 2 cut(s) 1275, 1547
MscI TGGCCA 1 cut(s) 1946
MseI TTAA 8 cut(s) 530, 686, 1173, 1530, 1869, 2165, 2405, 2468
MslI CAYNNNNRTG 4 cut(s) 1067, 1432, 1544, 2363
Msp20I TGGCCA 1 cut(s) 1946
MspA1I CMGCKG 2 cut(s) 1414, 1853
MspR9I CCNGG 4 cut(s) 942, 1925, 2318, 2379
MunI CAATTG 1 cut(s) 1725
Mva1269I GAATGC 2 cut(s) 1337, 2525
MvaI CCWGG 2 cut(s) 942, 1925
MvnI CGCG 4 cut(s) 69, 116, 1853, 2642
MwoI GCNNNNNNNGC 5 cut(s) 122, 445, 1161, 1559, 2288
NciI CCSGG 2 cut(s) 2318, 2379
NdeI CATATG 2 cut(s) 25, 2684
NlaIV GGNNCC 8 cut(s) 433, 865, 1006, 1588, 1620, 1708, 1936, 2322
NmeAIII GCCGAG 1 cut(s) 285
NmuCI GTSAC 6 cut(s) 109, 1123, 2013, 2728, 2755, 2819
NsiI ATGCAT 2 cut(s) 6, 31
NspI RCATGY 3 cut(s) 751, 1257, 2479
NspV TTCGAA 1 cut(s) 1269
OliI CACNNNNGTG 1 cut(s) 2363
PaeI GCATGC 1 cut(s) 2479
PaeR7I CTCGAG 1 cut(s) 2675
PagI TCATGA 1 cut(s) 1539
PciI ACATGT 1 cut(s) 747
PctI GAATGC 2 cut(s) 1337, 2525
PdmI GAANNNNTTC 2 cut(s) 1275, 1547
PfeI GAWTC 7 cut(s) 157, 734, 817, 1246, 1536, 2032, 2723
PkrI GCNGC 7 cut(s) 120, 311, 1300, 1852, 1855, 2481, 2782
PleI GAGTC 4 cut(s) 2261, 2710, 2752, 2843
PpsI GAGTC 4 cut(s) 2261, 2710, 2752, 2843
PscI ACATGT 1 cut(s) 747
PshBI ATTAAT 1 cut(s) 686
PsiI TTATAA 1 cut(s) 1914
Psp124BI GAGCTC 3 cut(s) 306, 1805, 2466
Psp6I CCWGG 2 cut(s) 940, 1923
PspEI GGTNACC 1 cut(s) 2381
PspGI CCWGG 2 cut(s) 940, 1923
PspN4I GGNNCC 8 cut(s) 433, 865, 1006, 1588, 1620, 1708, 1936, 2322
PspPI GGNCC 3 cut(s) 794, 1445, 1586
PstI CTGCAG 1 cut(s) 106
PstNI CAGNNNCTG 1 cut(s) 1721
PsuI RGATCY 4 cut(s) 863, 955, 1402, 1618
PvuII CAGCTG 1 cut(s) 1414
RsaI GTAC 7 cut(s) 880, 1105, 1110, 1366, 1792, 2399, 2841
RsaNI GTAC 7 cut(s) 879, 1104, 1109, 1365, 1791, 2398, 2840
RseI CAYNNNNRTG 4 cut(s) 1067, 1432, 1544, 2363
SacI GAGCTC 3 cut(s) 306, 1805, 2466
SacII CCGCGG 1 cut(s) 1854
SaqAI TTAA 8 cut(s) 530, 686, 1173, 1530, 1869, 2165, 2405, 2468
SatI GCNGC 7 cut(s) 119, 310, 1299, 1851, 1854, 2480, 2781
Sau96I GGNCC 3 cut(s) 794, 1445, 1586
ScaI AGTACT 2 cut(s) 1105, 2399
SchI GAGTC 4 cut(s) 2261, 2711, 2752, 2844
ScrFI CCNGG 4 cut(s) 942, 1925, 2318, 2379
SduI GDGCHC 7 cut(s) 79, 306, 1675, 1805, 1960, 2466, 2738
SfaNI GCATC 8 cut(s) 13, 1059, 1562, 1692, 1929, 2202, 2685, 2797
SfcI CTRYAG 4 cut(s) 102, 981, 1281, 2802
Sfr274I CTCGAG 1 cut(s) 2675
Sfr303I CCGCGG 1 cut(s) 1854
SfuI TTCGAA 1 cut(s) 1269
SgrBI CCGCGG 1 cut(s) 1854
SinI GGWCC 2 cut(s) 1445, 1586
SlaI CTCGAG 1 cut(s) 2675
SmiMI CAYNNNNRTG 4 cut(s) 1067, 1432, 1544, 2363
SmlI CTYRAG 4 cut(s) 1881, 2602, 2615, 2675
SmoI CTYRAG 4 cut(s) 1881, 2602, 2615, 2675
SpeI ACTAGT 1 cut(s) 2738
SphI GCATGC 1 cut(s) 2479
SspMI CTAG 4 cut(s) 1661, 1691, 2130, 2739
SstI GAGCTC 3 cut(s) 306, 1805, 2466
StyD4I CCNGG 4 cut(s) 940, 1923, 2316, 2377
StyI CCWWGG 4 cut(s) 393, 605, 2187, 2443
TaiI ACGT 5 cut(s) 47, 1789, 1957, 2799, 2811
TaqI TCGA 8 cut(s) 1220, 1269, 1407, 1450, 1602, 2236, 2509, 2676
TatI WGTACW 4 cut(s) 1103, 1108, 1364, 2397
TauI GCSGC 3 cut(s) 312, 1853, 1856
TfiI GAWTC 7 cut(s) 157, 734, 817, 1246, 1536, 2032, 2723
Tru1I TTAA 8 cut(s) 530, 686, 1173, 1530, 1869, 2165, 2405, 2468
Tru9I TTAA 8 cut(s) 530, 686, 1173, 1530, 1869, 2165, 2405, 2468
TscAI CASTG 6 cut(s) 1132, 1237, 1579, 2151, 2733, 2824
TseFI GTSAC 6 cut(s) 109, 1123, 2013, 2728, 2755, 2819
TseI GCWGC 4 cut(s) 118, 1298, 2479, 2780
Tsp45I GTSAC 6 cut(s) 109, 1123, 2013, 2728, 2755, 2819
TspGWI ACGGA 4 cut(s) 876, 1458, 1947, 2435
TspRI CASTG 6 cut(s) 1132, 1237, 1579, 2151, 2733, 2824
VneI GTGCAC 1 cut(s) 1956
VpaK11BI GGWCC 2 cut(s) 1445, 1586
VspI ATTAAT 1 cut(s) 686
XapI RAATTY 3 cut(s) 526, 579, 1458
XceI RCATGY 3 cut(s) 751, 1257, 2479
XcmI CCANNNNNNNNNTGG 2 cut(s) 1020, 2260
XhoI CTCGAG 1 cut(s) 2675
XmiI GTMKAC 1 cut(s) 364
XmnI GAANNNNTTC 2 cut(s) 1275, 1547
XspI CTAG 4 cut(s) 1661, 1691, 2130, 2739
ZraI GACGTC 2 cut(s) 1787, 2809
ZrmI AGTACT 2 cut(s) 1105, 2399
Zsp2I ATGCAT 2 cut(s) 6, 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.