MD03G1048600.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005593
Physical Location & Seq
Reverse (-)
3886665 .. 3893077
6413 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1048600.v1.1.491

Sequence Viewer

Length: 2610 bp
ATGCTCATAGTTGTGACGGCGCTGCGAGCCATCAAAGGAAGTTTGGTTGATCCCAACAAGAATCTGAGCAACTGGAATCAAGGAGACCCATGCACAGCAAATTGGACAGGAGTGTTTTGCTACAACAAAACATTAGATGATGGCTATCTTCATGTTCAAGAACTGCGACTACTAAATATGAACCTGTCAGGAAGTTTATCGCCAGAGCTCGGCCGCTTATCTTATATGAAGATACTGAATTTTATGTGGAACAAAATAAGTGGAAGTATACCAAAGGAGATAGGCAATATTACATCCTTGGAACTTTTGCTCCTCAATGGAAACCAGTTATCAGGTTCCTTGCCCGAAAAGCTTGGTTATCTTCCAAAATTGGACAGATTACAAATTGACGAGAACCAAATATCGGGATCAATACCTATATCGTTTGCAAATTTAAACAAGACAAGGCATTTTCACATGAACAACAATTCAATAAGCGGGCAAATTCCACCGGAGCTTTCCAGATTACCAAGGCTTATTCACTTTCTTCTTGACAACAACAACTTATCTGGGTATCTTCCACCGCAGTTCTCGGAGTTGCCAAGCTTATTAATACTTCAACTTGACAACAACAACTTTGAAGGGACTACAATTCCAGATTCGTATAGCGACATGTCTAAACTGTTGAAGTTGAGTTTGAGGAACTGCAGCTTACAAGGGCCTATTCCTGATTTGAGTAGGATTCCAAGCCTTGCTTATATAGACCTAAGCTCAAATCGGCTAAACGGATCCTTGCCTCAGGGTACACTTTCTGATAACATCACGACGATCAATTTATCTAACAACACTCTGACCGGAACAATTCCTGGCAACTTTTCAGATCTTCCTAATCTTCAGAGATTGTCTATAGCAAACAATTCATTGAACGGCTCCATTCCAGCAACGCTTTGGCAAAACCGGAAACTCAACAAGACAGAAAGACTAATCATGGAGATGCAGAACAATAAGCTTACAAATATTACCGGCAGTACTGTACTTCCGCAAAATGTCACTGTCTGGCTTCGGGGAAATCCGGTTTGCTCAAATGGCAACCTTGTTAACTTCTGCGGAACAGAAAGTGATGATGAAAATGACAGTCATATTTCGACTAACTCCACTGCCAACTGTCCGAATCAAGCATGTCCACCCGCTTTCGAATATATTCCTGTAGCTTGTTTTTGTGCTGCTCCTCTAATTGTTGACATTCGGTTGAAAAGTCCCGCATTCACAGATTTTCGCCCTTACGAAAGTACATATGAGCAGTATCTTACTTCTGGTATCAAATTAGATCTCGATCAGCTGGACATTACTTCATTTGTATGGGAAAATGGACCTCGACTGAGAATATCTCTGAAGCTTTTTCCTGTATATGTTGATAATGCCAATAATTCCCATACTTTCAATAGAACTGAAGTTAAAAAAATCATGAATATGTTCGCATCATGGGCAATTCCTCGCAGTGACCTATTTGGACCCTATGAACTTCTCGACTTCATTCTACTGGATCCTTACAAGGATGCAACCAAGAGCTCCATAAAAATTGAAGGTGTCAAGGATTTTACTTTTAGAGAAATGGCCACGGCTACAAACAACTTTAGCAGCTCTGCTCAAATTGGACAAGGAGGTTATGGAAATGTTTATAAAGGCATCCTGGCTGACGGAACCGTTGTGGCCATAAAACGTGCACAAGGGGGATCATTACAGGGCGAGAAGGAGTTTCTAACAGAAATAGAATTATTGTCACCCTTACATCATAGGAATCTCGTGTCCTTAGTTGGGTATTGTGATGAAAAAGGGGAACAGATGTTGGTTTATGAGTTTATGTCCAATGGAACTCTAAGGGATCACCTTTCTGCCGCTAGGTCTAAAGAACCACTGGGTTTTGAGATGAGATTAAGAATTGCTCAGGGATCAGCCAAGGGCATCCTATACCTCCATACAGAAGCCAACCCTCCGATATTTCATCGAGATATCAAGGCCACCAATATATTATTGGACTCTAAGTTCATTGCAAAGGTTGCTGATTTTGGCCTTTCCCGTCTTGCCCCGGTTCCAGATTTTGAAGGGACTGTGTCTTCTCATGTATCCACAGTAGTGAAAGGGACACCGGGTTACCTTGATCCAGAATACTTCTTAACTCATATGCTAACGGATAAGAGCGATGTTTATAGCCTCGGTGTTGTGTTTTTGGAGCTCTTAACAGGCATGCAGCCAATCTCACATGGAAAAAACATTGTTCGAGAGGTAAACATTGCATTCCAATCCGGTATGATCTTCTCAGTTATTGATGGAAGAATGGGGTCTTATCCTTCCGAGTGTGTGGAAAAGTTCTTGAGTTTGGCTCTCAAGTGTTGCAAAGTGGAGACAGACGCGAGACCCTCAATGGCGGAGGTTGTTCGGGAACTCGAGAACATATGGTTTATGATGCCGGAGTCTGACAGCAGAACATCGGAATCAGTGACGAGCAGTAGTGGTGGAAAGGTAGTGACTCTTCCACCCTCATCCAATGCAGCGAGGCATCCTTACGTCTCTATAGACGTCTCTGGCAGTGACCTTGTTAGCCGAGTCGTACCGACAATTACACCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

870

Amino Acids

96.06

Weight (kDa)

5.91

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 6 - 40 1.8e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 55 - 144 6.7e-06 Leucine-rich repeat region
LRR_8 PF13855 266 - 316 6.8e-07 Leucine rich repeat
Pkinase PF00069 540 - 806 1.5e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 541 - 808 3e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1659
AatII GACGTC 1 cut(s) 2559
AccI GTMKAC 1 cut(s) 268
AccII CGCG 1 cut(s) 2390
AciI CCGC 9 cut(s) 214, 477, 563, 1019, 1086, 1167, 1239, 1875, 2405
AcoI YGGCCR 3 cut(s) 211, 1593, 1689
AcsI RAATTY 3 cut(s) 238, 430, 483
AcuI CTGAAG 3 cut(s) 857, 1391, 1449
AcyI GRCGYC 1 cut(s) 2556
AfaI GTAC 5 cut(s) 784, 1009, 1014, 1270, 2589
AfiI CCNNNNNNNGG 3 cut(s) 209, 403, 1794
AflIII ACRYGT 1 cut(s) 651
AjnI CCWGG 2 cut(s) 844, 1668
AleI CACNNNNGTG 1 cut(s) 2111
AloI GAACNNNNNNTCC 4 cut(s) 294, 326, 2006, 2038
Alw21I GWGCWC 4 cut(s) 210, 1550, 1705, 2214
Alw26I GTCTC 5 cut(s) 78, 2375, 2386, 2551, 2563
Alw44I GTGCAC 1 cut(s) 1701
Ama87I CYCGRG 1 cut(s) 2423
AoxI GGCC 6 cut(s) 211, 698, 1593, 1689, 1995, 2047
ApaLI GTGCAC 1 cut(s) 1701
ApeKI GCWGC 6 cut(s) 22, 687, 1202, 1617, 2227, 2528
ApoI RAATTY 3 cut(s) 238, 430, 483
AseI ATTAAT 1 cut(s) 590
Asp700I GAANNNNTTC 2 cut(s) 1179, 1451
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 3 cut(s) 698, 1349, 1490
AsuC2I CCSGG 2 cut(s) 2066, 2127
AsuHPI GGTGA 2 cut(s) 1752, 1856
AsuII TTCGAA 1 cut(s) 1173
AvaI CYCGRG 1 cut(s) 2423
AvaII GGWCC 2 cut(s) 1349, 1490
AxyI CCTNAGG 1 cut(s) 777
BaeGI GKGCMC 1 cut(s) 1705
BalI TGGCCA 2 cut(s) 1595, 1691
BamHI GGATCC 2 cut(s) 767, 1522
BanII GRGCYC 3 cut(s) 210, 1550, 2214
BarI GAAGNNNNNNTAC 2 cut(s) 2493, 2525
BauI CACGAG 1 cut(s) 1781
BbsI GAAGAC 1 cut(s) 2085
Bbv12I GWGCWC 4 cut(s) 210, 1550, 1705, 2214
BbvI GCAGC 6 cut(s) 9, 699, 1189, 1629, 2239, 2540
BccI CCATC 3 cut(s) 38, 134, 2300
BceAI ACGGC 3 cut(s) 33, 922, 1614
BciT130I CCWGG 2 cut(s) 846, 1670
BciVI GTATCC 1 cut(s) 2113
BcnI CCSGG 2 cut(s) 2066, 2127
BcoDI GTCTC 5 cut(s) 78, 2375, 2386, 2551, 2563
BfaI CTAG 1 cut(s) 1878
BfmI CTRYAG 4 cut(s) 685, 885, 1185, 2550
BfoI RGCGCY 1 cut(s) 23
BfuI GTATCC 1 cut(s) 2113
BglII AGATCT 2 cut(s) 859, 1306
BisI GCNGC 8 cut(s) 23, 214, 688, 1203, 1618, 1875, 2228, 2529
BlsI GCNGC 8 cut(s) 24, 215, 689, 1204, 1619, 1876, 2229, 2530
BmcAI AGTACT 1 cut(s) 1009
Bme1390I CCNGG 4 cut(s) 846, 1670, 2066, 2127
Bme18I GGWCC 2 cut(s) 1349, 1490
BmeT110I CYCGRG 1 cut(s) 2423
BmgT120I GGNCC 3 cut(s) 698, 1349, 1490
BmiI GGNNCC 7 cut(s) 337, 769, 910, 1492, 1524, 1681, 2070
BmrFI CCNGG 4 cut(s) 846, 1670, 2066, 2127
BmrI ACTGGG 1 cut(s) 1904
BmsI GCATC 7 cut(s) 963, 1466, 1525, 1674, 1950, 2433, 2545
BmuI ACTGGG 1 cut(s) 1904
BpiI GAAGAC 1 cut(s) 2085
BplI GAGNNNNNCTC 4 cut(s) 1351, 1383, 2344, 2376
Bpu10I CCTNAGC 2 cut(s) 746, 1923
Bpu14I TTCGAA 1 cut(s) 1173
BpuEI CTTGAG 2 cut(s) 2348, 2371
BpuMI CCSGG 2 cut(s) 2066, 2127
BsaBI GATNNNNATC 2 cut(s) 144, 1311
BsaHI GRCGYC 1 cut(s) 2556
BsaI GGTCTC 2 cut(s) 78, 2386
BsaJI CCNNGG 6 cut(s) 297, 509, 1596, 1935, 2064, 2191
BsaWI WCCGGW 5 cut(s) 490, 833, 936, 1051, 2282
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 403, 1794
Bse118I RCCGGY 1 cut(s) 1001
Bse1I ACTGG 4 cut(s) 77, 325, 1524, 1899
Bse21I CCTNAGG 1 cut(s) 777
Bse3DI GCAATG 2 cut(s) 2025, 2268
Bse8I GATNNNNATC 2 cut(s) 144, 1311
BseBI CCWGG 2 cut(s) 846, 1670
BseDI CCNNGG 6 cut(s) 297, 509, 1596, 1935, 2064, 2191
BseGI GGATG 6 cut(s) 293, 1540, 1665, 1941, 2519, 2536
BseJI GATNNNNATC 2 cut(s) 144, 1311
BseLI CCNNNNNNNGG 3 cut(s) 209, 403, 1794
BseMI GCAATG 2 cut(s) 2025, 2268
BseMII CTCAG 5 cut(s) 56, 791, 1349, 1937, 2310
BseNI ACTGG 4 cut(s) 77, 325, 1524, 1899
BseRI GAGGAG 2 cut(s) 302, 1197
BseSI GKGCMC 1 cut(s) 1705
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 6 cut(s) 9, 699, 1189, 1629, 2239, 2540
Bsh1236I CGCG 1 cut(s) 2390
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 6 cut(s) 213, 700, 1595, 1691, 1997, 2049
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 4 cut(s) 210, 1550, 1705, 2214
BsiHKCI CYCGRG 1 cut(s) 2423
BsiSI CCGG 9 cut(s) 491, 834, 937, 1002, 1052, 2066, 2126, 2283, 2447
BslFI GGGAC 4 cut(s) 637, 1221, 2098, 2134
BslI CCNNNNNNNGG 3 cut(s) 209, 403, 1794
BsmAI GTCTC 5 cut(s) 78, 2375, 2386, 2551, 2563
BsmBI CGTCTC 2 cut(s) 2551, 2563
BsmFI GGGAC 4 cut(s) 637, 1221, 2098, 2134
BsmI GAATGC 2 cut(s) 1241, 2273
BsnI GGCC 6 cut(s) 213, 700, 1595, 1691, 1997, 2049
Bso31I GGTCTC 2 cut(s) 78, 2386
BsoBI CYCGRG 1 cut(s) 2423
Bsp119I TTCGAA 1 cut(s) 1173
Bsp1286I GDGCHC 4 cut(s) 210, 1550, 1705, 2214
BspACI CCGC 9 cut(s) 214, 477, 563, 1019, 1086, 1167, 1239, 1875, 2405
BspANI GGCC 6 cut(s) 213, 700, 1595, 1691, 1997, 2049
BspCNI CTCAG 5 cut(s) 57, 790, 1350, 1936, 2309
BspFNI CGCG 1 cut(s) 2390
BspHI TCATGA 1 cut(s) 1443
BspLI GGNNCC 7 cut(s) 337, 769, 910, 1492, 1524, 1681, 2070
BspMAI CTGCAG 1 cut(s) 689
BspT104I TTCGAA 1 cut(s) 1173
BspTNI GGTCTC 2 cut(s) 78, 2386
BsrDI GCAATG 2 cut(s) 2025, 2268
BsrFI RCCGGY 1 cut(s) 1001
BsrI ACTGG 4 cut(s) 77, 325, 1524, 1899
BssAI RCCGGY 1 cut(s) 1001
BssECI CCNNGG 6 cut(s) 297, 509, 1596, 1935, 2064, 2191
BssNAI GTATAC 1 cut(s) 269
BssNI GRCGYC 1 cut(s) 2556
BssSI CACGAG 1 cut(s) 1781
BssT1I CCWWGG 3 cut(s) 297, 509, 1935
Bst1107I GTATAC 1 cut(s) 269
Bst2BI CACGAG 1 cut(s) 1781
Bst2UI CCWGG 2 cut(s) 846, 1670
Bst4CI ACNGT 8 cut(s) 663, 1012, 1033, 1115, 1145, 1684, 2089, 2110
Bst6I CTCTTC 1 cut(s) 2514
BstACI GRCGYC 1 cut(s) 2556
BstAPI GCANNNNNTGC 1 cut(s) 2036
BstBI TTCGAA 1 cut(s) 1173
BstC8I GCNNGC 3 cut(s) 27, 479, 2225
BstDEI CTNAG 9 cut(s) 65, 746, 777, 1358, 1789, 1856, 1923, 2019, 2296
BstDSI CCRYGG 1 cut(s) 1596
BstEII GGTNACC 1 cut(s) 2129
BstF5I GGATG 6 cut(s) 293, 1540, 1665, 1941, 2519, 2536
BstFNI CGCG 1 cut(s) 2390
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstMAI GTCTC 5 cut(s) 78, 2375, 2386, 2551, 2563
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 5 cut(s) 26, 349, 1065, 1463, 2036
BstNI CCWGG 2 cut(s) 846, 1670
BstNSI RCATGY 3 cut(s) 655, 1161, 2227
BstPI GGTNACC 1 cut(s) 2129
BstSCI CCNGG 4 cut(s) 844, 1668, 2064, 2125
BstSFI CTRYAG 4 cut(s) 685, 885, 1185, 2550
BstSLI GKGCMC 1 cut(s) 1705
BstUI CGCG 1 cut(s) 2390
BstV1I GCAGC 6 cut(s) 9, 699, 1189, 1629, 2239, 2540
BstV2I GAAGAC 1 cut(s) 2085
BstX2I RGATCY 4 cut(s) 767, 859, 1306, 1522
BstYI RGATCY 4 cut(s) 767, 859, 1306, 1522
BstZ17I GTATAC 1 cut(s) 269
BstZI CGGCCG 1 cut(s) 211
Bsu36I CCTNAGG 1 cut(s) 777
BsuI GTATCC 1 cut(s) 2113
BsuRI GGCC 6 cut(s) 213, 700, 1595, 1691, 1997, 2049
BtgI CCRYGG 1 cut(s) 1596
BtgZI GCGATG 1 cut(s) 2193
BtsCI GGATG 6 cut(s) 293, 1540, 1665, 1941, 2519, 2536
BtsI GCAGTG 3 cut(s) 1134, 1483, 2572
BtsIMutI CAGTG 6 cut(s) 1029, 1134, 1483, 1892, 2481, 2572
Cac8I GCNNGC 3 cut(s) 27, 479, 2225
CciI TCATGA 1 cut(s) 1443
CfoI GCGC 1 cut(s) 22
Cfr10I RCCGGY 1 cut(s) 1001
Cfr13I GGNCC 3 cut(s) 698, 1349, 1490
CseI GACGC 1 cut(s) 2396
Csp6I GTAC 5 cut(s) 783, 1008, 1013, 1269, 2588
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviQI GTAC 5 cut(s) 783, 1008, 1013, 1269, 2588
DdeI CTNAG 9 cut(s) 65, 746, 777, 1358, 1789, 1856, 1923, 2019, 2296
DraI TTTAAA 1 cut(s) 435
EaeI YGGCCR 3 cut(s) 211, 1593, 1689
EagI CGGCCG 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 2514
EarI CTCTTC 1 cut(s) 2514
EciI GGCGGA 1 cut(s) 2420
Ecl136II GAGCTC 3 cut(s) 208, 1548, 2212
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 3 cut(s) 297, 509, 1935
Eco24I GRGCYC 3 cut(s) 210, 1550, 2214
Eco31I GGTCTC 2 cut(s) 78, 2386
Eco32I GATATC 1 cut(s) 1990
Eco47I GGWCC 2 cut(s) 1349, 1490
Eco52I CGGCCG 1 cut(s) 211
Eco53kI GAGCTC 3 cut(s) 208, 1548, 2212
Eco57I CTGAAG 3 cut(s) 857, 1391, 1449
Eco81I CCTNAGG 1 cut(s) 777
Eco88I CYCGRG 1 cut(s) 2423
Eco91I GGTNACC 1 cut(s) 2129
EcoICRI GAGCTC 3 cut(s) 208, 1548, 2212
EcoO109I RGGNCCY 1 cut(s) 698
EcoO65I GGTNACC 1 cut(s) 2129
EcoRII CCWGG 2 cut(s) 844, 1668
EcoRV GATATC 1 cut(s) 1990
EcoT14I CCWWGG 3 cut(s) 297, 509, 1935
EcoT38I GRGCYC 3 cut(s) 210, 1550, 2214
ErhI CCWWGG 3 cut(s) 297, 509, 1935
Esp3I CGTCTC 2 cut(s) 2551, 2563
FalI AAGNNNNNCTT 2 cut(s) 718, 750
FaqI GGGAC 4 cut(s) 637, 1221, 2098, 2134
FauI CCCGC 3 cut(s) 470, 1174, 1246
FauNDI CATATG 3 cut(s) 1273, 2160, 2432
FblI GTMKAC 1 cut(s) 268
Fnu4HI GCNGC 8 cut(s) 23, 214, 688, 1203, 1618, 1875, 2228, 2529
FokI GGATG 6 cut(s) 280, 1547, 1652, 1928, 2506, 2523
FriOI GRGCYC 3 cut(s) 210, 1550, 2214
Fsp4HI GCNGC 8 cut(s) 23, 214, 688, 1203, 1618, 1875, 2228, 2529
FspBI CTAG 1 cut(s) 1878
GlaI GCGC 1 cut(s) 21
GluI GCNGC 8 cut(s) 23, 214, 688, 1203, 1618, 1875, 2228, 2529
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 6 cut(s) 213, 700, 1595, 1691, 1997, 2049
HapII CCGG 9 cut(s) 491, 834, 937, 1002, 1052, 2066, 2126, 2283, 2447
HgaI GACGC 1 cut(s) 2396
HhaI GCGC 1 cut(s) 22
Hin1I GRCGYC 1 cut(s) 2556
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HincII GTYRAC 2 cut(s) 1078, 1219
HindII GTYRAC 2 cut(s) 1078, 1219
HindIII AAGCTT 4 cut(s) 350, 583, 986, 1373
HpaI GTTAAC 1 cut(s) 1078
HpaII CCGG 9 cut(s) 491, 834, 937, 1002, 1052, 2066, 2126, 2283, 2447
HphI GGTGA 2 cut(s) 1752, 1856
Hpy166II GTNNAC 7 cut(s) 269, 785, 1078, 1163, 1219, 1703, 2266
Hpy8I GTNNAC 7 cut(s) 269, 785, 1078, 1163, 1219, 1703, 2266
Hpy99I CGWCG 1 cut(s) 808
HpyAV CCTTC 5 cut(s) 614, 1556, 1723, 2075, 2337
HpyCH4III ACNGT 8 cut(s) 663, 1012, 1033, 1115, 1145, 1684, 2089, 2110
HpyCH4IV ACGT 3 cut(s) 1699, 2544, 2556
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 349, 1065, 1463, 2036
HpyF3I CTNAG 9 cut(s) 65, 746, 777, 1358, 1789, 1856, 1923, 2019, 2296
HpySE526I ACGT 3 cut(s) 1699, 2544, 2556
Hsp92I GRCGYC 1 cut(s) 2556
HspAI GCGC 1 cut(s) 20
KspAI GTTAAC 1 cut(s) 1078
LmnI GCTCC 6 cut(s) 315, 493, 914, 1210, 1553, 2209
Lsp1109I GCAGC 6 cut(s) 9, 699, 1189, 1629, 2239, 2540
LweI GCATC 7 cut(s) 963, 1466, 1525, 1674, 1950, 2433, 2545
MaeI CTAG 1 cut(s) 1878
MaeII ACGT 3 cut(s) 1699, 2544, 2556
MaeIII GTNAC 8 cut(s) 13, 1027, 1478, 1758, 2129, 2476, 2503, 2567
MflI RGATCY 4 cut(s) 767, 859, 1306, 1522
MhlI GDGCHC 4 cut(s) 210, 1550, 1705, 2214
MlsI TGGCCA 2 cut(s) 1595, 1691
MluNI TGGCCA 2 cut(s) 1595, 1691
MlyI GAGTC 4 cut(s) 2009, 2459, 2500, 2592
Mox20I TGGCCA 2 cut(s) 1595, 1691
MroXI GAANNNNTTC 2 cut(s) 1179, 1451
MscI TGGCCA 2 cut(s) 1595, 1691
MseI TTAA 7 cut(s) 434, 590, 1077, 1434, 1913, 2153, 2216
MslI CAYNNNNRTG 5 cut(s) 11, 971, 1336, 1448, 2111
Msp20I TGGCCA 2 cut(s) 1595, 1691
MspA1I CMGCKG 1 cut(s) 1318
MspI CCGG 9 cut(s) 491, 834, 937, 1002, 1052, 2066, 2126, 2283, 2447
MspR9I CCNGG 4 cut(s) 846, 1670, 2066, 2127
Mva1269I GAATGC 2 cut(s) 1241, 2273
MvaI CCWGG 2 cut(s) 846, 1670
MvnI CGCG 1 cut(s) 2390
MwoI GCNNNNNNNGC 5 cut(s) 26, 349, 1065, 1463, 2036
NciI CCSGG 2 cut(s) 2066, 2127
NdeI CATATG 3 cut(s) 1273, 2160, 2432
NlaIV GGNNCC 7 cut(s) 337, 769, 910, 1492, 1524, 1681, 2070
NmeAIII GCCGAG 2 cut(s) 189, 2606
NmuCI GTSAC 7 cut(s) 13, 1027, 1478, 1758, 2476, 2503, 2567
NspI RCATGY 3 cut(s) 655, 1161, 2227
NspV TTCGAA 1 cut(s) 1173
OliI CACNNNNGTG 1 cut(s) 2111
PaeI GCATGC 1 cut(s) 2227
PaeR7I CTCGAG 1 cut(s) 2423
PagI TCATGA 1 cut(s) 1443
PciI ACATGT 1 cut(s) 651
PctI GAATGC 2 cut(s) 1241, 2273
PdmI GAANNNNTTC 2 cut(s) 1179, 1451
PfeI GAWTC 7 cut(s) 61, 76, 638, 721, 1150, 1777, 2471
PflFI GACNNNGTC 1 cut(s) 2089
PkrI GCNGC 8 cut(s) 24, 215, 689, 1204, 1619, 1876, 2229, 2530
PleI GAGTC 4 cut(s) 2009, 2458, 2500, 2591
PpsI GAGTC 4 cut(s) 2009, 2458, 2500, 2591
PscI ACATGT 1 cut(s) 651
PshBI ATTAAT 1 cut(s) 590
PsiI TTATAA 1 cut(s) 1659
Psp124BI GAGCTC 3 cut(s) 210, 1550, 2214
Psp6I CCWGG 2 cut(s) 844, 1668
PspEI GGTNACC 1 cut(s) 2129
PspGI CCWGG 2 cut(s) 844, 1668
PspN4I GGNNCC 7 cut(s) 337, 769, 910, 1492, 1524, 1681, 2070
PspPI GGNCC 3 cut(s) 698, 1349, 1490
PsrI GAACNNNNNNTAC 2 cut(s) 153, 185
PstI CTGCAG 1 cut(s) 689
PsuI RGATCY 4 cut(s) 767, 859, 1306, 1522
PsyI GACNNNGTC 1 cut(s) 2089
PvuII CAGCTG 1 cut(s) 1318
RsaI GTAC 5 cut(s) 784, 1009, 1014, 1270, 2589
RsaNI GTAC 5 cut(s) 783, 1008, 1013, 1269, 2588
RseI CAYNNNNRTG 5 cut(s) 11, 971, 1336, 1448, 2111
SacI GAGCTC 3 cut(s) 210, 1550, 2214
SaqAI TTAA 7 cut(s) 434, 590, 1077, 1434, 1913, 2153, 2216
SatI GCNGC 8 cut(s) 23, 214, 688, 1203, 1618, 1875, 2228, 2529
Sau96I GGNCC 3 cut(s) 698, 1349, 1490
ScaI AGTACT 1 cut(s) 1009
SchI GAGTC 4 cut(s) 2009, 2459, 2500, 2592
ScrFI CCNGG 4 cut(s) 846, 1670, 2066, 2127
SduI GDGCHC 4 cut(s) 210, 1550, 1705, 2214
SfaNI GCATC 7 cut(s) 963, 1466, 1525, 1674, 1950, 2433, 2545
SfcI CTRYAG 4 cut(s) 685, 885, 1185, 2550
Sfr274I CTCGAG 1 cut(s) 2423
SfuI TTCGAA 1 cut(s) 1173
SinI GGWCC 2 cut(s) 1349, 1490
SlaI CTCGAG 1 cut(s) 2423
SmiMI CAYNNNNRTG 5 cut(s) 11, 971, 1336, 1448, 2111
SmlI CTYRAG 3 cut(s) 2350, 2363, 2423
SmoI CTYRAG 3 cut(s) 2350, 2363, 2423
SphI GCATGC 1 cut(s) 2227
SsiI CCGC 9 cut(s) 214, 477, 563, 1019, 1086, 1167, 1239, 1875, 2405
SspI AATATT 2 cut(s) 289, 997
SspMI CTAG 1 cut(s) 1878
SstI GAGCTC 3 cut(s) 210, 1550, 2214
StyD4I CCNGG 4 cut(s) 844, 1668, 2064, 2125
StyI CCWWGG 3 cut(s) 297, 509, 1935
TaaI ACNGT 8 cut(s) 663, 1012, 1033, 1115, 1145, 1684, 2089, 2110
TaiI ACGT 3 cut(s) 1702, 2547, 2559
TaqI TCGA 8 cut(s) 1124, 1173, 1311, 1354, 1506, 1984, 2257, 2424
TatI WGTACW 3 cut(s) 1007, 1012, 1268
TauI GCSGC 2 cut(s) 216, 1877
TfiI GAWTC 7 cut(s) 61, 76, 638, 721, 1150, 1777, 2471
Tru1I TTAA 7 cut(s) 434, 590, 1077, 1434, 1913, 2153, 2216
Tru9I TTAA 7 cut(s) 434, 590, 1077, 1434, 1913, 2153, 2216
TscAI CASTG 6 cut(s) 1036, 1141, 1483, 1899, 2481, 2572
TseFI GTSAC 7 cut(s) 13, 1027, 1478, 1758, 2476, 2503, 2567
TseI GCWGC 6 cut(s) 22, 687, 1202, 1617, 2227, 2528
Tsp45I GTSAC 7 cut(s) 13, 1027, 1478, 1758, 2476, 2503, 2567
TspGWI ACGGA 3 cut(s) 780, 1692, 2183
TspRI CASTG 6 cut(s) 1036, 1141, 1483, 1899, 2481, 2572
Tth111I GACNNNGTC 1 cut(s) 2089
VneI GTGCAC 1 cut(s) 1701
VpaK11BI GGWCC 2 cut(s) 1349, 1490
VspI ATTAAT 1 cut(s) 590
XapI RAATTY 3 cut(s) 238, 430, 483
XceI RCATGY 3 cut(s) 655, 1161, 2227
XcmI CCANNNNNNNNNTGG 2 cut(s) 924, 2008
XhoI CTCGAG 1 cut(s) 2423
XmiI GTMKAC 1 cut(s) 268
XmnI GAANNNNTTC 2 cut(s) 1179, 1451
XspI CTAG 1 cut(s) 1878
ZraI GACGTC 1 cut(s) 2557
ZrmI AGTACT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.