Rw5G044840
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005593
Physical Location & Seq
Forward (+)
78556631 .. 78564151
7521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044840.1

Sequence Viewer

Length: 2871 bp
ATGGGTCAATCTAGAGCTTGGACGTGTGTGGCCGTTTTGGCCGTGTGCTTGTGCTGGTCTTCACTACAAGCTGGAGCACAAGAGCAGGATATTACCAACCCAGTAGAAGTGATAGCATTGAAGGCCATAAAGAAAAGTTTGATTGATCCCAACAAGAATCTGAGCAATTGGAACCAAGGGGATCCATGTACAGCAAACTGGACAGGCGTTTTGTGCTTCAACAGATCATTAGATGATGGATATCTTCATGTTCAAGAATTGCAACTGCTAGGTATGAATCTCTCAGGAACTTTGTCACCAGAGCTTGGCCGCCTATCTTATATGATAATATTGGATTTTATGTGGAACGAAATAAGTGGAAGTGTACCAAAGGAGATAGGCAACATTACATCTTTGGAACTCTTACTCCTGAATGGAAACCAATTGTCAGGTCCCCTCCCTGAAGAGCTTGGTTATCTTCCGAACTTGGACAGAATACAAATTGACCAGAACCACATTTCAGGACCAATACCTAAATCATTCGCATATTTGAACAAAACGAAGCACTTTCACATGAACAACAATTCAATTAGCGGGCAACTTCCATCTGAGCTTTCCAGATTACCAAAACTTGTCCACATTCTTCTTGACAACAACAACTTGTCTGGGTATCTTCCACCAGAGTTCTCAGATCTGCCGAGCTTATTAATACTTCAACTTGACAACAACAACTTTGGTGGGAGTACAATTCCAGTTTCTTATGGCAACATGTCTAAACTGTTGAAGTTGAGTCTCAGGAACTGCAGCTTGGAAGGACCTATTCCTGATTTCAGTAGGATACCCAACCTTGGATATCTAGATCTAAGTAGGAATCGACTAAATGGATCCATACCTCTGGGAAAACTTTCGGATGACATCACAACGATAAATTTATCTAACAACACTCTTACTGGGAGCATTCCTGCCAACTTTTCCGGTCTTCCACAGCTTCAAAGATTGTCCATTGCAAACAATTCACTGAGTGGTATTGTTCCAGCAGCCCTTTGGAATGATACGACACTTAATGCAACAGAAAGACTAATCCTGGAGTTGCAGAACAATAAGCTTACAAATATATCTGGCAGTACTCAAACCCCGCAGAATGTCACTGTCTGGCTTCAGGGAAATCCAGTTTGCTCGAATGCTAACCTAGACAATTTATGTGGATCTGAAAATGATGATGAAGATGACAGTGAGAGTTCAACCAACTCGACTGCTAGCTGTCCAAGTCAAGCATGCCCGCCCCCTTTCGAATATCTTCCTGTAGTTTGTTTCTGTGCTGTTCCACTACTTATTGATTATCGGTTGAAAAGTCCTGGATTCTCAGATTTTCGCCCTTACAAAAGTACATTTGAGAAGTATCTTACGACTGGTCTCCTCTTAGATCTTGATCAACTGGACATTGCTTCATTTGTATGGCAAAAGGGACCTCGACTGAAAATATCACTGAAGCTTTTCCCTTTATATGTTGCTGATAATACCAATATTTCCCATACTTTCAATAGCAGTGAAGTCAAAAGAATCCTTGGCATGTTCACAGGATGGAATATTCCCGATAGTGAAGTGTTCGGACCTTATGAGCTTCTTGGCATCAATCTATTGGATCCTTATAAGGGCGTAAATGTCCCCTCTAAGAAGTCTGGTGTAAGCAAGGGTGCTCTGGCTGGCGTAATAGTAGGAACTTTTGCCGGTGCTGTTGCATTATCTGCAGTTGTCTGTTTTTTAATACTGAGAAGGCATCAGAGGAACCACCCTGCTATTTCAAGAAGACGTCGTAAATCGACGAGTTCCATAAAAATTGATGGTGTTAAGGCTTTCACTTACGGAGAAATGGCCACTGCTACAGACAATTTTAACATTTCTGCTCAAATTGGCCAAGGTGGTTATGGAAAGGTTTACAAAGGCACTCTGGCTGATGGCACACTTGTGGCCATAAAACGTGCACAAGAAGGATCGTTACAGGGTGAGAAGGAGTTTCTAACAGAAATAGAACTATTGTCACCCTTACATCATAGGAATCTGGTCTCCTTAGTTGGATATTGTGATGATGAAGGTGAACAGATGTTGGTTTATGAGTTCATGTCGCATGGCACTCTAAGGGATCACCTCTCTGCAGTTAAGTCGAAAGAACCTCTGGGATTTGAAATGAGATTGAGAATTGCTCTGGCATCAGCCAGGGGCATCCTCTACCTACATACAGAAGCCAACCCTCCGATATTCCATCGAGATATCAAGGCCAGCAACATATTATTGGACTCCAAGTTTGTAGCAAAGGTTGCTGATTTTGGACTTTCCCGACTTGCCCCAGTACCTGATCTTGAAGGGACTACGCCTGCTCATGTATCCACAGTAGTGAAGGGGACGCCAGGTTACCTTGATCCGGAGTACTTCTTAACCCATAAACTAACAGACAAGAGTGATGTTTATAGCCTCGGTGTTGTGTTTCTCGAGCTCTTAACTGGAATGCAGCCAATCTCACATGGAAAAAACATTGTTCGAGAGGTAAATATTGCATTCCAATCTGGTATGATCTTCTCAGTAATTGATGGGCGAATGGGGTCTTATCCTTCTGACTGTGTGGAAAAGTTCTTGAGTTTAGCCCTCAAGTGTTGTCAAGATGAGACAGATGCAAGACCCTCAATGGCAGAGGTGGTTCGAGAACTCGAAAACATATGGTCTATGATGCCGGAGACGGACAGCAGAACAACGGAATCAGTGCTTAGTAGTAGTACTGGAAAGGTAGTGAGCGATTCACCATCTTCATCCAATGCAGGGAAGAATCCCTATGTTTCTTCAGATGTCTCCGGCAGTGACCTCGTTAGTGGAGTCATACCTACAATCACACCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

956

Amino Acids

104.36

Weight (kDa)

5.68

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 72 7.2e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 154 - 214 7.5e-07 Leucine rich repeat
LRR_8 PF13855 300 - 342 2.8e-06 Leucine rich repeat
Pkinase PF00069 625 - 892 4e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 626 - 894 1.2e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1629
AatII GACGTC 1 cut(s) 1792
AccB7I CCANNNNNTGG 1 cut(s) 305
AccIII TCCGGA 1 cut(s) 2398
AciI CCGC 4 cut(s) 310, 573, 1115, 1259
AcoI YGGCCR 6 cut(s) 30, 39, 307, 1851, 1891, 1947
AcsI RAATTY 1 cut(s) 907
AcuI CTGAAG 4 cut(s) 462, 1121, 1487, 2796
AcyI GRCGYC 2 cut(s) 1789, 2381
AdeI CACNNNGTG 1 cut(s) 1001
AfaI GTAC 8 cut(s) 190, 366, 724, 1105, 1366, 2328, 2405, 2749
AfiI CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2398, 2790
AflIII ACRYGT 2 cut(s) 23, 747
AjiI CACGTC 1 cut(s) 24
AjnI CCWGG 4 cut(s) 1062, 1333, 2192, 2383
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AleI CACNNNNGTG 2 cut(s) 1943, 2369
AloI GAACNNNNNNTCC 2 cut(s) 390, 422
Alw21I GWGCWC 4 cut(s) 79, 1678, 1963, 2472
Alw26I GTCTC 6 cut(s) 776, 1397, 2047, 2633, 2702, 2824
Alw44I GTGCAC 1 cut(s) 1959
AlwNI CAGNNNCTG 2 cut(s) 780, 2330
Ama87I CYCGRG 1 cut(s) 2465
Aor13HI TCCGGA 1 cut(s) 2398
AoxI GGCC 8 cut(s) 30, 39, 123, 307, 1851, 1891, 1947, 2253
ApaLI GTGCAC 1 cut(s) 1959
ApeKI GCWGC 3 cut(s) 783, 1016, 2485
ApoI RAATTY 1 cut(s) 907
ArsI GACNNNNNNTTYG 4 cut(s) 193, 225, 2264, 2296
AseI ATTAAT 1 cut(s) 686
Asp700I GAANNNNTTC 3 cut(s) 883, 1275, 1472
AspS9I GGNCC 5 cut(s) 431, 503, 794, 1445, 1589
AsuHPI GGTGA 6 cut(s) 288, 1994, 2010, 2084, 2114, 2763
AsuII TTCGAA 1 cut(s) 1269
AsuNHI GCTAGC 1 cut(s) 1235
AvaI CYCGRG 1 cut(s) 2465
AvaII GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
BaeGI GKGCMC 1 cut(s) 1963
BalI TGGCCA 3 cut(s) 1853, 1893, 1949
BamHI GGATCC 3 cut(s) 181, 863, 1621
BanII GRGCYC 1 cut(s) 2472
BarI GAAGNNNNNNTAC 2 cut(s) 1959, 1991
BbsI GAAGAC 3 cut(s) 51, 950, 1792
Bbv12I GWGCWC 4 cut(s) 79, 1678, 1963, 2472
BbvI GCAGC 3 cut(s) 795, 1028, 2497
BccI CCATC 8 cut(s) 230, 592, 1554, 1814, 1928, 2247, 2558, 2782
BceAI ACGGC 2 cut(s) 17, 26
BcgI CGANNNNNNTGC 4 cut(s) 2121, 2155, 2815, 2849
BciT130I CCWGG 4 cut(s) 1064, 1335, 2194, 2385
BciVI GTATCC 2 cut(s) 810, 2371
BclI TGATCA 1 cut(s) 1408
BcoDI GTCTC 6 cut(s) 776, 1397, 2047, 2633, 2702, 2824
BfaI CTAG 5 cut(s) 12, 269, 836, 1169, 1236
BfmI CTRYAG 5 cut(s) 781, 1281, 1725, 1860, 2130
BfuI GTATCC 2 cut(s) 810, 2371
BglI GCCNNNNNGGC 1 cut(s) 38
BglII AGATCT 3 cut(s) 670, 838, 1402
BisI GCNGC 4 cut(s) 310, 784, 1017, 2486
BlsI GCNGC 4 cut(s) 311, 785, 1018, 2487
BmcAI AGTACT 3 cut(s) 1105, 2405, 2749
Bme1390I CCNGG 4 cut(s) 1064, 1335, 2194, 2385
Bme18I GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
BmeT110I CYCGRG 1 cut(s) 2465
BmgBI CACGTC 1 cut(s) 24
BmgT120I GGNCC 5 cut(s) 431, 503, 794, 1445, 1589
BmiI GGNNCC 7 cut(s) 173, 183, 433, 865, 1446, 1623, 1766
BmrFI CCNGG 4 cut(s) 1064, 1335, 2194, 2385
BmrI ACTGGG 3 cut(s) 95, 939, 2318
BmsI GCATC 6 cut(s) 1617, 1765, 2195, 2208, 2635, 2691
BmtI GCTAGC 1 cut(s) 1239
BmuI ACTGGG 3 cut(s) 95, 939, 2318
BpiI GAAGAC 3 cut(s) 51, 950, 1792
BplI GAGNNNNNCTC 2 cut(s) 2164, 2196
BpmI CTGGAG 2 cut(s) 93, 1085
Bpu14I TTCGAA 1 cut(s) 1269
BpuEI CTTGAG 2 cut(s) 2606, 2629
BsaBI GATNNNNATC 2 cut(s) 240, 1407
BsaHI GRCGYC 2 cut(s) 1789, 2381
BsaI GGTCTC 2 cut(s) 1397, 2047
BsaJI CCNNGG 6 cut(s) 175, 826, 1543, 1894, 2193, 2449
BsaWI WCCGGW 2 cut(s) 953, 2398
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2398, 2790
Bse118I RCCGGY 1 cut(s) 1706
Bse3DI GCAATG 2 cut(s) 981, 1419
Bse8I GATNNNNATC 2 cut(s) 240, 1407
BseAI TCCGGA 1 cut(s) 2398
BseBI CCWGG 4 cut(s) 1064, 1335, 2194, 2385
BseDI CCNNGG 6 cut(s) 175, 826, 1543, 1894, 2193, 2449
BseGI GGATG 4 cut(s) 895, 1565, 2199, 2780
BseJI GATNNNNATC 2 cut(s) 240, 1407
BseLI CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2398, 2790
BseMI GCAATG 2 cut(s) 981, 1419
BseMII CTCAG 9 cut(s) 152, 297, 579, 681, 787, 989, 1356, 1739, 2568
BseRI GAGGAG 1 cut(s) 1385
BseSI GKGCMC 1 cut(s) 1963
BseXI GCAGC 3 cut(s) 795, 1028, 2497
BshFI GGCC 8 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2255
BsiHKAI GWGCWC 4 cut(s) 79, 1678, 1963, 2472
BsiHKCI CYCGRG 1 cut(s) 2465
BsiSI CCGG 5 cut(s) 954, 1707, 2399, 2705, 2823
BslFI GGGAC 5 cut(s) 417, 1458, 1628, 2356, 2392
BslI CCNNNNNNNGG 5 cut(s) 305, 827, 1631, 2398, 2790
BsmAI GTCTC 6 cut(s) 776, 1397, 2047, 2633, 2702, 2824
BsmBI CGTCTC 1 cut(s) 2702
BsmFI GGGAC 5 cut(s) 417, 1458, 1628, 2356, 2392
BsmI GAATGC 4 cut(s) 936, 1165, 2487, 2531
BsnI GGCC 8 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2255
Bso31I GGTCTC 2 cut(s) 1397, 2047
BsoBI CYCGRG 1 cut(s) 2465
Bsp119I TTCGAA 1 cut(s) 1269
Bsp1286I GDGCHC 4 cut(s) 79, 1678, 1963, 2472
Bsp13I TCCGGA 1 cut(s) 2398
Bsp1407I TGTACA 1 cut(s) 188
BspACI CCGC 4 cut(s) 310, 573, 1115, 1259
BspANI GGCC 8 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2255
BspCNI CTCAG 9 cut(s) 153, 296, 580, 680, 786, 990, 1355, 1740, 2567
BspEI TCCGGA 1 cut(s) 2398
BspLI GGNNCC 7 cut(s) 173, 183, 433, 865, 1446, 1623, 1766
BspMAI CTGCAG 3 cut(s) 785, 1729, 2134
BspOI GCTAGC 1 cut(s) 1239
BspQI GCTCTTC 1 cut(s) 438
BspT104I TTCGAA 1 cut(s) 1269
BspTNI GGTCTC 2 cut(s) 1397, 2047
BsrDI GCAATG 2 cut(s) 981, 1419
BsrFI RCCGGY 1 cut(s) 1706
BsrGI TGTACA 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 1706
BssECI CCNNGG 6 cut(s) 175, 826, 1543, 1894, 2193, 2449
BssNI GRCGYC 2 cut(s) 1789, 2381
BssT1I CCWWGG 4 cut(s) 175, 826, 1543, 1894
Bst2UI CCWGG 4 cut(s) 1064, 1335, 2194, 2385
Bst4CI ACNGT 5 cut(s) 759, 1129, 1211, 2368, 2594
Bst6I CTCTTC 1 cut(s) 438
BstACI GRCGYC 2 cut(s) 1789, 2381
BstAPI GCANNNNNTGC 2 cut(s) 1724, 2294
BstAUI TGTACA 1 cut(s) 188
BstBI TTCGAA 1 cut(s) 1269
BstC8I GCNNGC 7 cut(s) 575, 1237, 1255, 1259, 1684, 2257, 2352
BstEII GGTNACC 1 cut(s) 2387
BstF5I GGATG 4 cut(s) 895, 1565, 2199, 2780
BstMAI GTCTC 6 cut(s) 776, 1397, 2047, 2633, 2702, 2824
BstMWI GCNNNNNNNGC 5 cut(s) 38, 122, 213, 1724, 2294
BstNI CCWGG 4 cut(s) 1064, 1335, 2194, 2385
BstNSI RCATGY 3 cut(s) 751, 1257, 1552
BstPI GGTNACC 1 cut(s) 2387
BstSCI CCNGG 4 cut(s) 1062, 1333, 2192, 2383
BstSFI CTRYAG 5 cut(s) 781, 1281, 1725, 1860, 2130
BstSLI GKGCMC 1 cut(s) 1963
BstV1I GCAGC 3 cut(s) 795, 1028, 2497
BstV2I GAAGAC 3 cut(s) 51, 950, 1792
BstX2I RGATCY 7 cut(s) 181, 670, 838, 863, 1184, 1402, 1621
BstXI CCANNNNNNTGG 1 cut(s) 874
BstYI RGATCY 7 cut(s) 181, 670, 838, 863, 1184, 1402, 1621
BsuI GTATCC 2 cut(s) 810, 2371
BsuRI GGCC 8 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2255
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 4 cut(s) 895, 1565, 2199, 2780
BtsI GCAGTG 3 cut(s) 1531, 1854, 2833
BtsIMutI CAGTG 8 cut(s) 995, 1125, 1216, 1463, 1531, 1854, 2739, 2833
Cac8I GCNNGC 7 cut(s) 575, 1237, 1255, 1259, 1684, 2257, 2352
CaiI CAGNNNCTG 2 cut(s) 780, 2330
Cfr10I RCCGGY 1 cut(s) 1706
Cfr13I GGNCC 5 cut(s) 431, 503, 794, 1445, 1589
CseI GACGC 1 cut(s) 2389
Csp6I GTAC 8 cut(s) 189, 365, 723, 1104, 1365, 2327, 2404, 2748
CspCI CAANNNNNGTGG 4 cut(s) 697, 732, 1162, 1197
CviQI GTAC 8 cut(s) 189, 365, 723, 1104, 1365, 2327, 2404, 2748
DraIII CACNNNGTG 1 cut(s) 1001
EaeI YGGCCR 6 cut(s) 30, 39, 307, 1851, 1891, 1947
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 2470
Eco130I CCWWGG 4 cut(s) 175, 826, 1543, 1894
Eco24I GRGCYC 1 cut(s) 2472
Eco31I GGTCTC 2 cut(s) 1397, 2047
Eco32I GATATC 3 cut(s) 242, 833, 2248
Eco47I GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
Eco53kI GAGCTC 1 cut(s) 2470
Eco57I CTGAAG 4 cut(s) 462, 1121, 1487, 2796
Eco88I CYCGRG 1 cut(s) 2465
Eco91I GGTNACC 1 cut(s) 2387
EcoICRI GAGCTC 1 cut(s) 2470
EcoO109I RGGNCCY 3 cut(s) 431, 794, 1445
EcoO65I GGTNACC 1 cut(s) 2387
EcoRII CCWGG 4 cut(s) 1062, 1333, 2192, 2383
EcoRV GATATC 3 cut(s) 242, 833, 2248
EcoT14I CCWWGG 4 cut(s) 175, 826, 1543, 1894
EcoT38I GRGCYC 1 cut(s) 2472
ErhI CCWWGG 4 cut(s) 175, 826, 1543, 1894
Esp3I CGTCTC 1 cut(s) 2702
FaqI GGGAC 5 cut(s) 417, 1458, 1628, 2356, 2392
FauI CCCGC 3 cut(s) 566, 1122, 1266
FauNDI CATATG 1 cut(s) 2690
FbaI TGATCA 1 cut(s) 1408
Fnu4HI GCNGC 4 cut(s) 310, 784, 1017, 2486
FokI GGATG 4 cut(s) 902, 1572, 2186, 2767
FriOI GRGCYC 1 cut(s) 2472
Fsp4HI GCNGC 4 cut(s) 310, 784, 1017, 2486
FspBI CTAG 5 cut(s) 12, 269, 836, 1169, 1236
GluI GCNGC 4 cut(s) 310, 784, 1017, 2486
GsuI CTGGAG 2 cut(s) 93, 1085
HaeIII GGCC 8 cut(s) 32, 41, 125, 309, 1853, 1893, 1949, 2255
HapII CCGG 5 cut(s) 954, 1707, 2399, 2705, 2823
HgaI GACGC 1 cut(s) 2389
Hin1I GRCGYC 2 cut(s) 1789, 2381
HindIII AAGCTT 2 cut(s) 1082, 1469
HpaII CCGG 5 cut(s) 954, 1707, 2399, 2705, 2823
HphI GGTGA 6 cut(s) 288, 1994, 2010, 2084, 2114, 2763
Hpy166II GTNNAC 6 cut(s) 365, 616, 1554, 1915, 1961, 2075
Hpy8I GTNNAC 6 cut(s) 365, 616, 1554, 1915, 1961, 2075
Hpy99I CGWCG 2 cut(s) 1794, 1804
HpyAV CCTTC 9 cut(s) 115, 785, 1746, 1961, 1981, 2063, 2333, 2368, 2595
HpyCH4III ACNGT 5 cut(s) 759, 1129, 1211, 2368, 2594
HpyCH4IV ACGT 3 cut(s) 23, 1789, 1957
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 122, 213, 1724, 2294
HpySE526I ACGT 3 cut(s) 23, 1789, 1957
Hsp92I GRCGYC 2 cut(s) 1789, 2381
Kpn2I TCCGGA 1 cut(s) 2398
Ksp22I TGATCA 1 cut(s) 1408
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 2 cut(s) 74, 933
Lsp1109I GCAGC 3 cut(s) 795, 1028, 2497
LweI GCATC 6 cut(s) 1617, 1765, 2195, 2208, 2635, 2691
MaeI CTAG 5 cut(s) 12, 269, 836, 1169, 1236
MaeII ACGT 3 cut(s) 23, 1789, 1957
MaeIII GTNAC 6 cut(s) 294, 1123, 1974, 2016, 2387, 2828
MfeI CAATTG 2 cut(s) 166, 422
MflI RGATCY 7 cut(s) 181, 670, 838, 863, 1184, 1402, 1621
MhlI GDGCHC 4 cut(s) 79, 1678, 1963, 2472
MlsI TGGCCA 3 cut(s) 1853, 1893, 1949
MluNI TGGCCA 3 cut(s) 1853, 1893, 1949
MlyI GAGTC 3 cut(s) 778, 2267, 2853
MmeI TCCRAC 1 cut(s) 2032
Mox20I TGGCCA 3 cut(s) 1853, 1893, 1949
MroI TCCGGA 1 cut(s) 2398
MroXI GAANNNNTTC 3 cut(s) 883, 1275, 1472
MscI TGGCCA 3 cut(s) 1853, 1893, 1949
MseI TTAA 8 cut(s) 686, 1041, 1742, 1827, 1872, 2136, 2411, 2474
MslI CAYNNNNRTG 3 cut(s) 1432, 1943, 2369
Msp20I TGGCCA 3 cut(s) 1853, 1893, 1949
MspI CCGG 5 cut(s) 954, 1707, 2399, 2705, 2823
MspR9I CCNGG 4 cut(s) 1064, 1335, 2194, 2385
MunI CAATTG 2 cut(s) 166, 422
Mva1269I GAATGC 4 cut(s) 936, 1165, 2487, 2531
MvaI CCWGG 4 cut(s) 1064, 1335, 2194, 2385
MwoI GCNNNNNNNGC 5 cut(s) 38, 122, 213, 1724, 2294
NdeI CATATG 1 cut(s) 2690
NheI GCTAGC 1 cut(s) 1235
NlaIV GGNNCC 7 cut(s) 173, 183, 433, 865, 1446, 1623, 1766
NmeAIII GCCGAG 1 cut(s) 702
NmuCI GTSAC 4 cut(s) 294, 1123, 2016, 2828
NspI RCATGY 3 cut(s) 751, 1257, 1552
NspV TTCGAA 1 cut(s) 1269
OliI CACNNNNGTG 2 cut(s) 1943, 2369
PaeI GCATGC 1 cut(s) 1257
PaeR7I CTCGAG 1 cut(s) 2465
PciI ACATGT 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 438
PctI GAATGC 4 cut(s) 936, 1165, 2487, 2531
PdmI GAANNNNTTC 3 cut(s) 883, 1275, 1472
PfeI GAWTC 9 cut(s) 157, 277, 850, 1338, 1539, 2035, 2729, 2768, 2797
PflMI CCANNNNNTGG 1 cut(s) 305
PfoI TCCNGGA 2 cut(s) 1062, 1333
PkrI GCNGC 4 cut(s) 311, 785, 1018, 2487
PleI GAGTC 3 cut(s) 777, 2267, 2852
PpsI GAGTC 3 cut(s) 777, 2267, 2852
PpuMI RGGWCCY 3 cut(s) 431, 794, 1445
PscI ACATGT 1 cut(s) 747
PshBI ATTAAT 1 cut(s) 686
PsiI TTATAA 1 cut(s) 1629
Psp124BI GAGCTC 1 cut(s) 2472
Psp5II RGGWCCY 3 cut(s) 431, 794, 1445
Psp6I CCWGG 4 cut(s) 1062, 1333, 2192, 2383
PspEI GGTNACC 1 cut(s) 2387
PspGI CCWGG 4 cut(s) 1062, 1333, 2192, 2383
PspN4I GGNNCC 7 cut(s) 173, 183, 433, 865, 1446, 1623, 1766
PspPI GGNCC 5 cut(s) 431, 503, 794, 1445, 1589
PspPPI RGGWCCY 3 cut(s) 431, 794, 1445
PstI CTGCAG 3 cut(s) 785, 1729, 2134
PstNI CAGNNNCTG 2 cut(s) 780, 2330
PsuI RGATCY 7 cut(s) 181, 670, 838, 863, 1184, 1402, 1621
RsaI GTAC 8 cut(s) 190, 366, 724, 1105, 1366, 2328, 2405, 2749
RsaNI GTAC 8 cut(s) 189, 365, 723, 1104, 1365, 2327, 2404, 2748
RseI CAYNNNNRTG 3 cut(s) 1432, 1943, 2369
SacI GAGCTC 1 cut(s) 2472
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 8 cut(s) 686, 1041, 1742, 1827, 1872, 2136, 2411, 2474
SatI GCNGC 4 cut(s) 310, 784, 1017, 2486
Sau96I GGNCC 5 cut(s) 431, 503, 794, 1445, 1589
ScaI AGTACT 3 cut(s) 1105, 2405, 2749
SchI GAGTC 3 cut(s) 778, 2267, 2853
ScrFI CCNGG 4 cut(s) 1064, 1335, 2194, 2385
SduI GDGCHC 4 cut(s) 79, 1678, 1963, 2472
SfaNI GCATC 6 cut(s) 1617, 1765, 2195, 2208, 2635, 2691
SfcI CTRYAG 5 cut(s) 781, 1281, 1725, 1860, 2130
SfiI GGCCNNNNNGGCC 1 cut(s) 38
Sfr274I CTCGAG 1 cut(s) 2465
SfuI TTCGAA 1 cut(s) 1269
SinI GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
SlaI CTCGAG 1 cut(s) 2465
SmiMI CAYNNNNRTG 3 cut(s) 1432, 1943, 2369
SmlI CTYRAG 3 cut(s) 2465, 2608, 2621
SmoI CTYRAG 3 cut(s) 2465, 2608, 2621
SphI GCATGC 1 cut(s) 1257
SsiI CCGC 4 cut(s) 310, 573, 1115, 1259
SspI AATATT 4 cut(s) 330, 1504, 1567, 2527
SspMI CTAG 5 cut(s) 12, 269, 836, 1169, 1236
SstI GAGCTC 1 cut(s) 2472
StyD4I CCNGG 4 cut(s) 1062, 1333, 2192, 2383
StyI CCWWGG 4 cut(s) 175, 826, 1543, 1894
TaaI ACNGT 5 cut(s) 759, 1129, 1211, 2368, 2594
TaiI ACGT 3 cut(s) 26, 1792, 1960
TatI WGTACW 6 cut(s) 188, 722, 1103, 1364, 2403, 2747
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 9 cut(s) 157, 277, 850, 1338, 1539, 2035, 2729, 2768, 2797
Tru1I TTAA 8 cut(s) 686, 1041, 1742, 1827, 1872, 2136, 2411, 2474
Tru9I TTAA 8 cut(s) 686, 1041, 1742, 1827, 1872, 2136, 2411, 2474
TscAI CASTG 8 cut(s) 1002, 1132, 1216, 1470, 1531, 1861, 2739, 2833
TseFI GTSAC 4 cut(s) 294, 1123, 2016, 2828
TseI GCWGC 3 cut(s) 783, 1016, 2485
Tsp45I GTSAC 4 cut(s) 294, 1123, 2016, 2828
TspDTI ATGAA 8 cut(s) 236, 290, 569, 1215, 1416, 2082, 2086, 2769
TspGWI ACGGA 3 cut(s) 1857, 2726, 2741
TspRI CASTG 8 cut(s) 1002, 1132, 1216, 1470, 1531, 1861, 2739, 2833
Van91I CCANNNNNTGG 1 cut(s) 305
VneI GTGCAC 1 cut(s) 1959
VpaK11BI GGWCC 5 cut(s) 431, 503, 794, 1445, 1589
VspI ATTAAT 1 cut(s) 686
XapI RAATTY 1 cut(s) 907
XbaI TCTAGA 2 cut(s) 11, 835
XceI RCATGY 3 cut(s) 751, 1257, 1552
XcmI CCANNNNNNNNNTGG 2 cut(s) 1020, 1901
XhoI CTCGAG 1 cut(s) 2465
XmnI GAANNNNTTC 3 cut(s) 883, 1275, 1472
XspI CTAG 5 cut(s) 12, 269, 836, 1169, 1236
ZraI GACGTC 1 cut(s) 1790
ZrmI AGTACT 3 cut(s) 1105, 2405, 2749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.