FvH4_3g41720

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004995
Physical Location & Seq
Forward (+)
34993685 .. 34996469
2785 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41720.t1

Sequence Viewer

Length: 687 bp
ATGGGGGCTGTAGTGGGAAAGGCTGAAAGCCCTAGAAGTCTTTCGATATCTGAGACAAAGCTGGAGGCCAAAATGGTTGAAGCAATGCAGAGAAGAGCAGCTAAAGGAAGTGTCATGAAATCATTCAACTCCATAATCTTGAAGTTCCCAAAGATTGATGAAAGCTTAAGAAATTGCAAAGATATTTTTCAGCAGTTTGATGAGGATTCAAATGGCGCAATCGATCATGATGAATTGAAAAGAGCTTTCTCAAAACTGGAAGTCTCGTTTACAGATGAGGAAATCGATGATCTCTTTGCAGCATGCGATATTAATGAGGATATGGGAATCAATTTTAATGAGTTCATTGTTCTTATTTGTGTCGTCTTTCTTCTGAAGGATGATCAGAATGCTGGTGAAAAAAAAGTGGATATGGGAATGCCAAAGCTGGAGGCCACATTCGAAACATTGGTGGATGCATTTGTGTTCTTGGATAAGAACAAGGATGGTTATGTCAGCAAGAGTGAGATGGTTCAGGCCATTAATGAAACCACAACAGGAGAACGCTCCTCTGGGCGAATAGCCATGAAAAGATTTGAAGAGATGGACTGGGATAGAAATGGAATGGTGAACTTCAAGGAGTTCCTTTTCGCATTCACTCGATGGATTGGAATTGACGATCTTGATGATGAGGATGAAGAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

229

Amino Acids

25.99

Weight (kDa)

4.49

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 59 - 116 2.2e-11 EF-hand domain pair
EF-hand_1 PF00036 59 - 86 3.2e-06 EF hand domain
EF-hand_6 PF13405 60 - 85 7.8e-06 EF-hand domain
EF-hand_8 PF13833 70 - 118 1.5e-06 EF-hand domain pair
EF-hand_7 PF13499 148 - 212 4.1e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 395
AflII CTTAAG 1 cut(s) 166
AgsI TTSAA 7 cut(s) 80, 127, 142, 210, 238, 578, 616
AluBI AGCT 5 cut(s) 61, 101, 165, 245, 427
AluI AGCT 5 cut(s) 61, 101, 165, 245, 427
Alw26I GTCTC 2 cut(s) 47, 268
AoxI GGCC 3 cut(s) 66, 432, 516
ApeKI GCWGC 2 cut(s) 98, 299
AseI ATTAAT 2 cut(s) 312, 522
Asp700I GAANNNNTTC 2 cut(s) 40, 122
AspLEI GCGC 1 cut(s) 218
AsuHPI GGTGA 2 cut(s) 407, 619
AsuII TTCGAA 1 cut(s) 441
BbvI GCAGC 2 cut(s) 110, 311
BccI CCATC 4 cut(s) 479, 502, 577, 636
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 2 cut(s) 47, 268
BfaI CTAG 1 cut(s) 33
BfmI CTRYAG 1 cut(s) 9
BfrI CTTAAG 1 cut(s) 166
BisI GCNGC 2 cut(s) 99, 300
BlsI GCNGC 2 cut(s) 100, 301
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 1 cut(s) 445
BmuI ACTGGG 1 cut(s) 598
BpmI CTGGAG 2 cut(s) 83, 449
Bpu14I TTCGAA 1 cut(s) 441
Bsa29I ATCGAT 2 cut(s) 222, 285
Bse1I ACTGG 2 cut(s) 261, 593
Bse3DI GCAATG 1 cut(s) 90
BseCI ATCGAT 2 cut(s) 222, 285
BseGI GGATG 4 cut(s) 385, 460, 490, 679
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 2 cut(s) 261, 593
BseRI GAGGAG 1 cut(s) 538
BseXI GCAGC 2 cut(s) 110, 311
BshFI GGCC 3 cut(s) 68, 434, 518
BshVI ATCGAT 2 cut(s) 222, 285
BsmAI GTCTC 2 cut(s) 47, 268
BsmI GAATGC 3 cut(s) 394, 423, 632
BsnI GGCC 3 cut(s) 68, 434, 518
Bsp119I TTCGAA 1 cut(s) 441
Bsp143I GATC 4 cut(s) 223, 289, 382, 658
BspANI GGCC 3 cut(s) 68, 434, 518
BspCNI CTCAG 1 cut(s) 43
BspDI ATCGAT 2 cut(s) 222, 285
BspHI TCATGA 2 cut(s) 114, 226
BspQI GCTCTTC 1 cut(s) 88
BspT104I TTCGAA 1 cut(s) 441
BspTI CTTAAG 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 90
BsrI ACTGG 2 cut(s) 261, 593
BssMI GATC 4 cut(s) 223, 289, 382, 658
Bst6I CTCTTC 3 cut(s) 88, 573, 672
BstAFI CTTAAG 1 cut(s) 166
BstBI TTCGAA 1 cut(s) 441
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 1 cut(s) 51
BstF5I GGATG 4 cut(s) 385, 460, 490, 679
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 4 cut(s) 226, 292, 385, 661
BstMAI GTCTC 2 cut(s) 47, 268
BstMBI GATC 4 cut(s) 223, 289, 382, 658
BstNSI RCATGY 1 cut(s) 306
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 110, 311
Bsu15I ATCGAT 2 cut(s) 222, 285
BsuRI GGCC 3 cut(s) 68, 434, 518
BsuTUI ATCGAT 2 cut(s) 222, 285
BtsCI GGATG 4 cut(s) 385, 460, 490, 679
Cac8I GCNNGC 1 cut(s) 304
CciI TCATGA 2 cut(s) 114, 226
CfoI GCGC 1 cut(s) 218
ClaI ATCGAT 2 cut(s) 222, 285
CviAII CATG 4 cut(s) 115, 227, 303, 565
DdeI CTNAG 1 cut(s) 51
DpnI GATC 4 cut(s) 225, 291, 384, 660
DpnII GATC 4 cut(s) 223, 289, 382, 658
Eam1104I CTCTTC 3 cut(s) 88, 573, 672
EarI CTCTTC 3 cut(s) 88, 573, 672
Eco32I GATATC 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 395
EcoRV GATATC 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 460
FaeI CATG 4 cut(s) 118, 230, 306, 568
FaiI YATR 8 cut(s) 116, 134, 228, 304, 323, 413, 492, 566
FatI CATG 4 cut(s) 114, 226, 302, 564
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 2 cut(s) 99, 300
FokI GGATG 3 cut(s) 392, 467, 497
Fsp4HI GCNGC 2 cut(s) 99, 300
FspBI CTAG 1 cut(s) 33
GlaI GCGC 1 cut(s) 217
GluI GCNGC 2 cut(s) 99, 300
GsuI CTGGAG 2 cut(s) 83, 449
HaeIII GGCC 3 cut(s) 68, 434, 518
HhaI GCGC 1 cut(s) 218
Hin1II CATG 4 cut(s) 118, 230, 306, 568
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 2 cut(s) 206, 327
HphI GGTGA 2 cut(s) 407, 619
Hpy166II GTNNAC 2 cut(s) 270, 610
Hpy188I TCNGA 3 cut(s) 52, 375, 387
Hpy188III TCNNGA 4 cut(s) 115, 139, 227, 662
Hpy8I GTNNAC 2 cut(s) 270, 610
HpyAV CCTTC 1 cut(s) 370
HpyCH4V TGCA 4 cut(s) 88, 177, 299, 458
HpyF3I CTNAG 1 cut(s) 51
Hsp92II CATG 4 cut(s) 118, 230, 306, 568
HspAI GCGC 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 4 cut(s) 223, 289, 382, 658
LguI GCTCTTC 1 cut(s) 88
LmnI GCTCC 1 cut(s) 551
LpnPI CCDG 8 cut(s) 47, 242, 378, 413, 500, 522, 537, 574
Lsp1109I GCAGC 2 cut(s) 110, 311
LweI GCATC 1 cut(s) 445
MaeI CTAG 1 cut(s) 33
MalI GATC 4 cut(s) 225, 291, 384, 660
MboI GATC 4 cut(s) 223, 289, 382, 658
MboII GAAGA 3 cut(s) 105, 362, 590
MluCI AATT 4 cut(s) 172, 233, 331, 651
MnlI CCTC 8 cut(s) 58, 196, 271, 310, 424, 559, 664, 673
Mph1103I ATGCAT 1 cut(s) 460
MroXI GAANNNNTTC 2 cut(s) 40, 122
MseI TTAA 4 cut(s) 167, 312, 336, 522
MspCI CTTAAG 1 cut(s) 166
Mva1269I GAATGC 3 cut(s) 394, 423, 632
NdeII GATC 4 cut(s) 223, 289, 382, 658
NlaIII CATG 4 cut(s) 118, 230, 306, 568
NsiI ATGCAT 1 cut(s) 460
NspI RCATGY 1 cut(s) 306
NspV TTCGAA 1 cut(s) 441
PaeI GCATGC 1 cut(s) 306
PagI TCATGA 2 cut(s) 114, 226
PciSI GCTCTTC 1 cut(s) 88
PctI GAATGC 3 cut(s) 394, 423, 632
PdmI GAANNNNTTC 2 cut(s) 40, 122
PfeI GAWTC 2 cut(s) 206, 327
PkrI GCNGC 2 cut(s) 100, 301
PshBI ATTAAT 2 cut(s) 312, 522
SapI GCTCTTC 1 cut(s) 88
SaqAI TTAA 4 cut(s) 167, 312, 336, 522
SatI GCNGC 2 cut(s) 99, 300
Sau3AI GATC 4 cut(s) 223, 289, 382, 658
SetI ASST 5 cut(s) 63, 103, 167, 247, 429
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 1 cut(s) 9
SfuI TTCGAA 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SphI GCATGC 1 cut(s) 306
Sse9I AATT 4 cut(s) 172, 233, 331, 651
SspMI CTAG 1 cut(s) 33
TaqI TCGA 5 cut(s) 44, 222, 285, 441, 640
TasI AATT 4 cut(s) 172, 233, 331, 651
TfiI GAWTC 2 cut(s) 206, 327
Tru1I TTAA 4 cut(s) 167, 312, 336, 522
Tru9I TTAA 4 cut(s) 167, 312, 336, 522
TseI GCWGC 2 cut(s) 98, 299
TspDTI ATGAA 6 cut(s) 131, 174, 246, 334, 540, 581
Vha464I CTTAAG 1 cut(s) 166
VspI ATTAAT 2 cut(s) 312, 522
XceI RCATGY 1 cut(s) 306
XmnI GAANNNNTTC 2 cut(s) 40, 122
XspI CTAG 1 cut(s) 33
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.