Rorug05G0434200

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004995
Physical Location & Seq
Reverse (-)
60306233 .. 60306703
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0434200.1

Sequence Viewer

Length: 471 bp
ATGGCATTGGGATTGCAGGGTTCAAAATCCCTCGGGGGCTTTGTTCTTCTGCTCCTCCTTGCTGCGATCGGCGCCGTGGCCGGAGCAGCGCCGGCCCCAAAATCCAGTAACAAAACAAGCATCGGAGAAGGAGGAGGAGGAAGCATCTACGATCACCTCCGGCAATATGGGTTACCCATCGGCCTCCTCCCCAAAGGCATCACGGAATATTCCCTCGACGATTCCAGCGGCGAATTCCGGGTGTTTCTGCCCCAGCCTTGCCGCGCCAAGTTCGAGAATCAGGTCCGGTACGATTTCAACGTCTCTGGGACTCTCACTTTCGGCCGAATCGCCGACTTATCCGGCGTCTCCGCTCAGGAACTCTTCCTCTGGTTTCTGGTGAAAGGTATCCGAGTCGATGTGCCCAGCTCTGGCCTCATTTACTTCAATGTCGGCGTTGTCGATAAGCAGTTCTCTTTGTCCCTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

156

Amino Acids

16.56

Weight (kDa)

7.83

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 49 - 156 4.1e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccBSI CCGCTC 1 cut(s) 353
AccII CGCG 1 cut(s) 264
AciI CCGC 3 cut(s) 228, 262, 351
AcoI YGGCCR 2 cut(s) 78, 322
AcsI RAATTY 1 cut(s) 233
AcyI GRCGYC 2 cut(s) 72, 345
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 3 cut(s) 24, 298, 427
AluBI AGCT 1 cut(s) 408
AluI AGCT 1 cut(s) 408
Alw26I GTCTC 2 cut(s) 307, 352
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 5 cut(s) 78, 93, 181, 322, 412
ApeKI GCWGC 2 cut(s) 62, 86
ApoI RAATTY 1 cut(s) 233
AspLEI GCGC 3 cut(s) 74, 91, 266
AspS9I GGNCC 2 cut(s) 94, 283
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 146, 391
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 1 cut(s) 283
BaeGI GKGCMC 1 cut(s) 405
BanI GGYRCC 1 cut(s) 71
BbvI GCAGC 2 cut(s) 49, 98
BccI CCATC 1 cut(s) 185
BceAI ACGGC 1 cut(s) 59
BciVI GTATCC 1 cut(s) 398
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 2 cut(s) 307, 352
BfaI CTAG 1 cut(s) 469
BfoI RGCGCY 2 cut(s) 75, 92
BfuI GTATCC 1 cut(s) 398
BisI GCNGC 4 cut(s) 63, 87, 229, 262
BlsI GCNGC 4 cut(s) 64, 88, 230, 263
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 1 cut(s) 283
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 2 cut(s) 94, 283
BmiI GGNNCC 2 cut(s) 73, 96
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 3 cut(s) 129, 153, 207
Bpu10I CCTNAGC 1 cut(s) 354
BpuMI CCSGG 1 cut(s) 239
BsaHI GRCGYC 2 cut(s) 72, 345
BsaJI CCNNGG 2 cut(s) 31, 75
BsaWI WCCGGW 1 cut(s) 285
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 31, 75
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 105
BseRI GAGGAG 4 cut(s) 44, 147, 150, 176
BseSI GKGCMC 1 cut(s) 405
BseX3I CGGCCG 1 cut(s) 322
BseXI GCAGC 2 cut(s) 49, 98
BseYI CCCAGC 2 cut(s) 252, 404
Bsh1236I CGCG 1 cut(s) 264
Bsh1285I CGRYCG 2 cut(s) 69, 325
BshFI GGCC 5 cut(s) 80, 95, 183, 324, 414
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 2 cut(s) 69, 325
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 6 cut(s) 81, 92, 160, 238, 286, 342
BslFI GGGAC 2 cut(s) 322, 445
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 2 cut(s) 307, 352
BsmBI CGTCTC 2 cut(s) 307, 352
BsmFI GGGAC 2 cut(s) 322, 445
BsnI GGCC 5 cut(s) 80, 95, 183, 324, 414
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 2 cut(s) 66, 151
BspACI CCGC 3 cut(s) 228, 262, 351
BspANI GGCC 5 cut(s) 80, 95, 183, 324, 414
BspCNI CTCAG 1 cut(s) 367
BspFNI CGCG 1 cut(s) 264
BspLI GGNNCC 2 cut(s) 73, 96
BspT107I GGYRCC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 353
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 1 cut(s) 105
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 2 cut(s) 31, 75
BssMI GATC 2 cut(s) 66, 151
BssNI GRCGYC 2 cut(s) 72, 345
Bst6I CTCTTC 1 cut(s) 368
BstACI GRCGYC 2 cut(s) 72, 345
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 1 cut(s) 75
BstEII GGTNACC 1 cut(s) 171
BstFNI CGCG 1 cut(s) 264
BstH2I RGCGCY 2 cut(s) 75, 92
BstHHI GCGC 3 cut(s) 74, 91, 266
BstKTI GATC 2 cut(s) 69, 154
BstMAI GTCTC 2 cut(s) 307, 352
BstMBI GATC 2 cut(s) 66, 151
BstMCI CGRYCG 2 cut(s) 69, 325
BstMWI GCNNNNNNNGC 3 cut(s) 71, 86, 92
BstPI GGTNACC 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 237
BstSLI GKGCMC 1 cut(s) 405
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 49, 98
BstZI CGGCCG 1 cut(s) 322
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 5 cut(s) 80, 95, 183, 324, 414
BtgI CCRYGG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 3 cut(s) 74, 91, 266
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 94, 283
CseI GACGC 1 cut(s) 334
Csp6I GTAC 1 cut(s) 289
CviJI RGCY 8 cut(s) 39, 80, 95, 183, 256, 324, 408, 414
CviKI_1 RGCY 8 cut(s) 39, 80, 95, 183, 256, 324, 408, 414
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 1 cut(s) 354
DinI GGCGCC 1 cut(s) 73
DpnI GATC 2 cut(s) 68, 153
DpnII GATC 2 cut(s) 66, 151
EaeI YGGCCR 2 cut(s) 78, 322
EagI CGGCCG 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
EclXI CGGCCG 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 283
Eco52I CGGCCG 1 cut(s) 322
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 171
EcoO65I GGTNACC 1 cut(s) 171
EcoRI GAATTC 1 cut(s) 233
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
Esp3I CGTCTC 2 cut(s) 307, 352
FaiI YATR 1 cut(s) 168
FaqI GGGAC 2 cut(s) 322, 445
Fnu4HI GCNGC 4 cut(s) 63, 87, 229, 262
Fsp4HI GCNGC 4 cut(s) 63, 87, 229, 262
FspBI CTAG 1 cut(s) 469
GlaI GCGC 3 cut(s) 73, 90, 265
GluI GCNGC 4 cut(s) 63, 87, 229, 262
GsaI CCCAGC 2 cut(s) 256, 408
HaeII RGCGCY 2 cut(s) 75, 92
HaeIII GGCC 5 cut(s) 80, 95, 183, 324, 414
HapII CCGG 6 cut(s) 81, 92, 160, 238, 286, 342
HgaI GACGC 1 cut(s) 334
HhaI GCGC 3 cut(s) 74, 91, 266
Hin1I GRCGYC 2 cut(s) 72, 345
Hin6I GCGC 3 cut(s) 72, 89, 264
HinP1I GCGC 3 cut(s) 72, 89, 264
HinfI GANTC 5 cut(s) 221, 277, 310, 327, 393
HpaII CCGG 6 cut(s) 81, 92, 160, 238, 286, 342
HphI GGTGA 2 cut(s) 146, 391
Hpy188I TCNGA 2 cut(s) 125, 392
Hpy188III TCNNGA 2 cut(s) 274, 356
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 1 cut(s) 122
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 1 cut(s) 16
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 86, 92
HpyF3I CTNAG 1 cut(s) 354
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 2 cut(s) 72, 345
HspAI GCGC 3 cut(s) 72, 89, 264
KasI GGCGCC 1 cut(s) 71
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 2 cut(s) 66, 151
LmnI GCTCC 2 cut(s) 57, 83
Lsp1109I GCAGC 2 cut(s) 49, 98
LweI GCATC 3 cut(s) 129, 153, 207
MaeI CTAG 1 cut(s) 469
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 2 cut(s) 107, 171
MalI GATC 2 cut(s) 68, 153
MbiI CCGCTC 1 cut(s) 353
MboI GATC 2 cut(s) 66, 151
MboII GAAGA 3 cut(s) 38, 355, 457
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 1 cut(s) 233
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 2 cut(s) 304, 402
MroNI GCCGGC 1 cut(s) 91
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 6 cut(s) 81, 92, 160, 238, 286, 342
MspR9I CCNGG 1 cut(s) 239
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 71, 86, 92
NaeI GCCGGC 1 cut(s) 93
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 239
NdeII GATC 2 cut(s) 66, 151
NgoMIV GCCGGC 1 cut(s) 91
NlaIV GGNNCC 2 cut(s) 73, 96
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 438
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 3 cut(s) 221, 277, 327
PkrI GCNGC 4 cut(s) 64, 88, 230, 263
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 2 cut(s) 304, 401
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 2 cut(s) 304, 401
PspEI GGTNACC 1 cut(s) 171
PspFI CCCAGC 2 cut(s) 252, 404
PspN4I GGNNCC 2 cut(s) 73, 96
PspPI GGNCC 2 cut(s) 94, 283
PvuI CGATCG 1 cut(s) 69
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SatI GCNGC 4 cut(s) 63, 87, 229, 262
Sau3AI GATC 2 cut(s) 66, 151
Sau96I GGNCC 2 cut(s) 94, 283
SchI GAGTC 2 cut(s) 304, 402
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 1 cut(s) 405
SetI ASST 5 cut(s) 159, 285, 303, 388, 410
SfaNI GCATC 3 cut(s) 129, 153, 207
SfoI GGCGCC 1 cut(s) 73
SinI GGWCC 1 cut(s) 283
Sse9I AATT 1 cut(s) 233
SsiI CCGC 3 cut(s) 228, 262, 351
SspDI GGCGCC 1 cut(s) 71
SspI AATATT 1 cut(s) 209
SspMI CTAG 1 cut(s) 469
StyD4I CCNGG 1 cut(s) 237
TaiI ACGT 1 cut(s) 303
TaqI TCGA 4 cut(s) 216, 273, 396, 441
TasI AATT 1 cut(s) 233
TauI GCSGC 2 cut(s) 231, 264
TfiI GAWTC 3 cut(s) 221, 277, 327
TseI GCWGC 2 cut(s) 62, 86
TspGWI ACGGA 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 1 cut(s) 233
XspI CTAG 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.