Rh5CG537000

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0004995
Physical Location & Seq
Forward (+)
75135307 .. 75142945
7639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG537000.1

Sequence Viewer

Length: 756 bp
ATGGGGGCTGTAGTGGGAAAGGCCAATAGTGGGAGTCTTTCGATATCTGAAACAAAGCTGGAAGCCAAAATGGTTGAAGCTATGCAGCGAAGAGCAGCTAAAGGAAGTGTCATGAAATCATTCAACAGCATAATCTTGAAGTTCCCAAAGATTGATGAGAGCTTAAGAAATTGCAAAGATATTTTTCAGCAGTTTGGTGTGTATCCATTCCATACTCCCCCCCCCCTAAAATGTCCCAAGTGGTTGTTAACTGTCATGATTTTCTCTACAGATGAGGATTCAAATGGCATCATTGATCATGATGAATTGAAAAGAGCTTTCTCAAAACTGGAAGTCTCGTTTACAGATGAGGAAATCGATGATCTCTTTGCAGCATGCGATATTAATGAGGATATGGGAATCAATTTTAATGAGTTCATCGTTCTTATTTGCGTCGTCTTTCTTCTGAAGGATGATCAGAATGCTGGGCAAAAAAAAGTGGATATGGGAATGCCAAGGCTGGAAGCCACCTTCGAAACATTGGTGGATGCATTTGTGTTCTTGGATAAGAACAAGGATGGTTATGTCAGCAAGAGTGAAATGGTTCAGTCCATTAATGAAACTACGACAGGAGAACGCTCCTCTGGGCGAATAGCCATGAAAAGATTTGAAGAGATGGACTGGGATAAAAATGGAATGGTGAACTTCAAGGAGTTCCTTTTTGCATTCACTCGATGGATTGGAATTGACGATCTTGAGGATGAAGATGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

251

Amino Acids

28.56

Weight (kDa)

4.64

Isoelectric Point (pI)

33.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 90 - 140 2.4e-07 EF-hand domain pair
EF-hand_8 PF13833 94 - 142 6.6e-07 EF-hand domain pair
EF-hand_7 PF13499 172 - 236 6.1e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 467
AfiI CCNNNNNNNGG 1 cut(s) 30
AflII CTTAAG 1 cut(s) 163
AgsI TTSAA 7 cut(s) 77, 124, 139, 282, 310, 650, 688
AluBI AGCT 5 cut(s) 58, 80, 98, 162, 317
AluI AGCT 5 cut(s) 58, 80, 98, 162, 317
Alw26I GTCTC 1 cut(s) 340
AoxI GGCC 1 cut(s) 21
ApeKI GCWGC 3 cut(s) 85, 95, 371
AseI ATTAAT 2 cut(s) 384, 594
Asp700I GAANNNNTTC 2 cut(s) 119, 582
AsuHPI GGTGA 1 cut(s) 691
AsuII TTCGAA 1 cut(s) 513
BbvI GCAGC 3 cut(s) 97, 107, 383
BccI CCATC 3 cut(s) 551, 649, 708
BciVI GTATCC 1 cut(s) 213
BclI TGATCA 2 cut(s) 295, 454
BcoDI GTCTC 1 cut(s) 340
BfmI CTRYAG 2 cut(s) 9, 267
BfrI CTTAAG 1 cut(s) 163
BfuI GTATCC 1 cut(s) 213
BisI GCNGC 3 cut(s) 86, 96, 372
BlsI GCNGC 3 cut(s) 87, 97, 373
BmrI ACTGGG 1 cut(s) 670
BmsI GCATC 2 cut(s) 297, 517
BmuI ACTGGG 1 cut(s) 670
Bpu14I TTCGAA 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 755
Bsa29I ATCGAT 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 494
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 333, 665
BseCI ATCGAT 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 494
BseGI GGATG 4 cut(s) 457, 532, 562, 745
BseLI CCNNNNNNNGG 1 cut(s) 30
BseNI ACTGG 2 cut(s) 333, 665
BseRI GAGGAG 1 cut(s) 610
BseXI GCAGC 3 cut(s) 97, 107, 383
BseYI CCCAGC 1 cut(s) 464
BshFI GGCC 1 cut(s) 23
BshVI ATCGAT 1 cut(s) 357
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 1 cut(s) 340
BsmFI GGGAC 1 cut(s) 219
BsmI GAATGC 3 cut(s) 466, 495, 704
BsnI GGCC 1 cut(s) 23
Bsp119I TTCGAA 1 cut(s) 513
Bsp143I GATC 4 cut(s) 295, 361, 454, 730
BspANI GGCC 1 cut(s) 23
BspDI ATCGAT 1 cut(s) 357
BspHI TCATGA 3 cut(s) 111, 255, 298
BspQI GCTCTTC 1 cut(s) 85
BspT104I TTCGAA 1 cut(s) 513
BspTI CTTAAG 1 cut(s) 163
BsrI ACTGG 2 cut(s) 333, 665
BssECI CCNNGG 1 cut(s) 494
BssMI GATC 4 cut(s) 295, 361, 454, 730
BssT1I CCWWGG 1 cut(s) 494
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 2 cut(s) 85, 645
BstAFI CTTAAG 1 cut(s) 163
BstBI TTCGAA 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 376
BstF5I GGATG 4 cut(s) 457, 532, 562, 745
BstKTI GATC 4 cut(s) 298, 364, 457, 733
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 4 cut(s) 295, 361, 454, 730
BstNSI RCATGY 1 cut(s) 378
BstSFI CTRYAG 2 cut(s) 9, 267
BstV1I GCAGC 3 cut(s) 97, 107, 383
Bsu15I ATCGAT 1 cut(s) 357
BsuI GTATCC 1 cut(s) 213
BsuRI GGCC 1 cut(s) 23
BsuTUI ATCGAT 1 cut(s) 357
BtsCI GGATG 4 cut(s) 457, 532, 562, 745
Cac8I GCNNGC 1 cut(s) 376
CciI TCATGA 3 cut(s) 111, 255, 298
ClaI ATCGAT 1 cut(s) 357
CseI GACGC 1 cut(s) 421
CviAII CATG 5 cut(s) 112, 256, 299, 375, 637
DpnI GATC 4 cut(s) 297, 363, 456, 732
DpnII GATC 4 cut(s) 295, 361, 454, 730
Eam1104I CTCTTC 2 cut(s) 85, 645
EarI CTCTTC 2 cut(s) 85, 645
Eco130I CCWWGG 1 cut(s) 494
Eco32I GATATC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 467
EcoRV GATATC 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 494
EcoT22I ATGCAT 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 494
FaeI CATG 5 cut(s) 115, 259, 302, 378, 640
FaqI GGGAC 1 cut(s) 219
FatI CATG 5 cut(s) 111, 255, 298, 374, 636
FbaI TGATCA 2 cut(s) 295, 454
Fnu4HI GCNGC 3 cut(s) 86, 96, 372
FokI GGATG 4 cut(s) 464, 539, 569, 752
Fsp4HI GCNGC 3 cut(s) 86, 96, 372
GluI GCNGC 3 cut(s) 86, 96, 372
GsaI CCCAGC 1 cut(s) 468
HaeIII GGCC 1 cut(s) 23
HgaI GACGC 1 cut(s) 421
Hin1II CATG 5 cut(s) 115, 259, 302, 378, 640
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HinfI GANTC 3 cut(s) 34, 278, 399
HpaI GTTAAC 1 cut(s) 249
HphI GGTGA 1 cut(s) 691
Hpy166II GTNNAC 3 cut(s) 249, 342, 682
Hpy188I TCNGA 3 cut(s) 49, 447, 459
Hpy188III TCNNGA 5 cut(s) 112, 136, 256, 299, 734
Hpy8I GTNNAC 3 cut(s) 249, 342, 682
Hpy99I CGWCG 1 cut(s) 437
HpyAV CCTTC 2 cut(s) 442, 520
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4V TGCA 5 cut(s) 85, 174, 371, 530, 704
Hsp92II CATG 5 cut(s) 115, 259, 302, 378, 640
Ksp22I TGATCA 2 cut(s) 295, 454
KspAI GTTAAC 1 cut(s) 249
Kzo9I GATC 4 cut(s) 295, 361, 454, 730
LguI GCTCTTC 1 cut(s) 85
LmnI GCTCC 1 cut(s) 623
LpnPI CCDG 7 cut(s) 44, 314, 450, 485, 594, 609, 646
Lsp1109I GCAGC 3 cut(s) 97, 107, 383
LweI GCATC 2 cut(s) 297, 517
MalI GATC 4 cut(s) 297, 363, 456, 732
MboI GATC 4 cut(s) 295, 361, 454, 730
MboII GAAGA 4 cut(s) 102, 434, 662, 755
MluCI AATT 4 cut(s) 169, 305, 403, 723
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 5 cut(s) 268, 343, 382, 631, 730
Mph1103I ATGCAT 1 cut(s) 532
MroXI GAANNNNTTC 2 cut(s) 119, 582
MseI TTAA 5 cut(s) 164, 248, 384, 408, 594
MspCI CTTAAG 1 cut(s) 163
Mva1269I GAATGC 3 cut(s) 466, 495, 704
NdeII GATC 4 cut(s) 295, 361, 454, 730
NlaIII CATG 5 cut(s) 115, 259, 302, 378, 640
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 1 cut(s) 378
NspV TTCGAA 1 cut(s) 513
PaeI GCATGC 1 cut(s) 378
PagI TCATGA 3 cut(s) 111, 255, 298
PciSI GCTCTTC 1 cut(s) 85
PctI GAATGC 3 cut(s) 466, 495, 704
PdmI GAANNNNTTC 2 cut(s) 119, 582
PfeI GAWTC 2 cut(s) 278, 399
PkrI GCNGC 3 cut(s) 87, 97, 373
PleI GAGTC 1 cut(s) 42
PpsI GAGTC 1 cut(s) 42
PshBI ATTAAT 2 cut(s) 384, 594
PspFI CCCAGC 1 cut(s) 464
SapI GCTCTTC 1 cut(s) 85
SaqAI TTAA 5 cut(s) 164, 248, 384, 408, 594
SatI GCNGC 3 cut(s) 86, 96, 372
Sau3AI GATC 4 cut(s) 295, 361, 454, 730
SchI GAGTC 1 cut(s) 43
SetI ASST 6 cut(s) 60, 82, 100, 164, 319, 512
SfaNI GCATC 2 cut(s) 297, 517
SfcI CTRYAG 2 cut(s) 9, 267
SfuI TTCGAA 1 cut(s) 513
SmlI CTYRAG 2 cut(s) 163, 734
SmoI CTYRAG 2 cut(s) 163, 734
SphI GCATGC 1 cut(s) 378
Sse9I AATT 4 cut(s) 169, 305, 403, 723
StyI CCWWGG 1 cut(s) 494
TaaI ACNGT 1 cut(s) 253
TaqI TCGA 4 cut(s) 41, 357, 513, 712
TasI AATT 4 cut(s) 169, 305, 403, 723
TfiI GAWTC 2 cut(s) 278, 399
Tru1I TTAA 5 cut(s) 164, 248, 384, 408, 594
Tru9I TTAA 5 cut(s) 164, 248, 384, 408, 594
TseI GCWGC 3 cut(s) 85, 95, 371
TspDTI ATGAA 6 cut(s) 128, 318, 406, 612, 653, 756
Vha464I CTTAAG 1 cut(s) 163
VspI ATTAAT 2 cut(s) 384, 594
XceI RCATGY 1 cut(s) 378
XmnI GAANNNNTTC 2 cut(s) 119, 582
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.